| regulation of the activity of microbial kynureninase by transamination of the enzyme-bound coenzyme. | kynureninase was purified to homogeneity from the extracts of pseudomonas marginalis and neurospora crassa. the active kynureninase containing pyridoxal 5'-phosphate transaminates with l-ornithine or l-alanine to form the inactive pyridoxamine 5'-phosphate form of enzyme and delta1-pyrroline-2-carboxylate or pyruvate. this inactive enzyme transaminates with pyruvate to restore the active pyridoxal 5'-phosphate enzyme and l-alanine. the activity of kynureninase is regulated in this manner by tran ... | 1975 | 1244119 |
| molecular cloning and nucleotide sequence of a pectin lyase gene from pseudomonas marginalis n6301. | a pectin lyase (pnl;ec4.2.2.10) gene of pseudomonas marginalis n6301 was cloned and expressed in escherichia coli. we purified pnl from p. marginalis n6301 and determined n-terminal 33 amino acids sequence. from this sequence, we synthesized two oligonucleotide probes. from the analysis of southern hybridization, 2. 1kb ecori-smai fragment from the chromosomal dna of p. marginalis was found to hybridize with oligonucleotide probes. then, we cloned the fragment into puc119 vector and transformed ... | 1992 | 1731776 |
| the structure of the acidic exopolysaccharide of pseudomonas marginalis strains pf-05-2 and pm-lb-1. | the structure of an acidic exopolysaccharide of two strains of pseudomonas marginalis, a bacterium which causes soft rots of various vegetables, has been determined to consist of a repeating unit of: ----4) beta-d-manp-(1----3)alpha-d-glcp-(1----4)alpha-l-rhap-(1-. the glucose is pyruvated at o-4 and o-6 and the mannose is acetylated at either o-2 or o-3. | 1990 | 2379201 |
| structure of an acidic exopolysaccharide of pseudomonas marginalis ht041b. | the exopolysaccharide of pseudomonas marginalis ht041b has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. the polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at either the 2 or 4 position of glucose. this polysaccharide has been given the trivial name marginalan. | 1989 | 2921250 |
| properties of crystalline kynureninase from pseudomonas marginalis. | | 1973 | 4730493 |
| crystalline kynureninase from pseudomonas marginalis. | | 1971 | 5123212 |
| pectic enzymes of pseudomonas marginalis. | | 1966 | 5980347 |
| mechanism-based inactivation of bacterial kynureninase by beta-substituted amino acids. | kynureninase from pseudomonas marginalis has been shown to catalyze the elimination of beta-functionalities of beta-substituted amino acids such as beta-chloro-l-alanine, resulting in the formation of aminoacrylate-pyridoxal phosphate-enzyme complex. this intermediate can be processed further to produce either pyruvate, ammonia, and active enzyme or an inactive enzyme complex. approximately 1 in 500 turnovers leads to inactivation of the enzyme. the mechanism of inactivation appears to involve n ... | 1984 | 6432787 |
| a method for the synthesis of [14c]-kynurenine. | a new method is described for the synthesis of [14c]-labelled l-kynurenine from [14c]-l-tryptophan, using extracts of tryptophan-adapted cells of pseudomonas marginalis. it is based on the selective, rapid inactivation of kynureninase by a newly discovered inhibitor of this enzyme, 3-chloro-l-alanine. the yield of [14c]-kynurenine produced in this manner is 76% theoretical. | 1984 | 6440137 |
| detection of beta-carbanion formation during kynurenine hydrolysis catalyzed by pseudomonas marginalis kynureninase. | 2-amino-4-hydroxy-4-phenylbutyric acid has been shown to be formed during the pseudomonas marginalis kynureninase-catalyzed hydrolysis of kynurenine in the presence of benzaldehyde and pyridoxal phosphate. the formation of 2-amino-4-hydroxy-4-phenylbutyric acid is the first demonstration, to our knowledge, of the controlled trapping of an amino acid beta-carbanion generated either chemically or enzymatically, and is perhaps the best empirical evidence to date that enzyme mechanisms can proceed t ... | 1984 | 6497394 |
| pseudomonas marginalis: its degradative capability on organic nitriles and amides. | pseudomonas marginalis, capable of utilizing acetonitrile as the sole source of carbon and nitrogen, was isolated from an industrial waste site. p. marginalis metabolized acetonitrile into ammonia and acetate. the minimal inhibitory concentration values of different nitriles and amides for p. marginalis were in the range 5-300 mm. the bacterium was able to transform high-molecular-mass nitrile compounds and their respective amides into ammonia. the data from substrate-dependent kinetics showed t ... | 1995 | 7546612 |
| molecular cloning and nucleotide sequence of the pectate lyase gene from pseudomonas marginalis n6301. | pectate lyase was purified approximately 29-fold to electrophoretic homogeneity from pseudomonas marginalis n6301. a pectate lyase (pl; ec4.2.2.2) gene of the strain was cloned and expressed in escherichia coli. the nucleotides of the pl gene (pel) were sequenced. an open reading frame that encodes a polypeptide (molecular weight: 40,812) composed of 380 amino acids including a 29 amino acid signal peptide was assigned. the structural gene of pel consisted of 1140 base pairs. the nucleotide sequ ... | 1993 | 7763884 |
| expression of a pectin lyase gene in escherichia coli from pseudomonas marginalis n6301. | we constructed deletion mutant clones of a pectin lyase gene, and measured their pectin lyase activities in escherichia coli. pectin lyase activities were detected only in a reca+ strain but not in a reca- strain of e. coli. we also cloned and sequenced reca from pseudomonas marginalis n6301. the reca from p. marginalis n6301 can complement reca- to form reca+ in the phenotype of e. coli. highly conserved sequences of reca are observed among e. coli, p. fluorescens, and p. marginalis. from these ... | 1994 | 7765726 |
| structure of the acidic exopolysaccharide of pseudomonas marginalis strain atcc 10844. | | 1993 | 8495442 |
| involvement of a surface-active high molecular weight factor in degradation of polycyclic aromatic hydrocarbons by pseudomonas marginalis. | a strain of pseudomonas marginalis pd-14b, isolated from polycyclic aromatic hydrocarbon (pah) contaminated soil, produced an extracellular surface-active factor of high molecular weight containing protein and lipopolysaccharide. a crude preparation of the factor, obtained from the culture both of cells grown in mineral salts (ms) medium with succinate and yeast extract, affected the extent of pah degradation by the bacterial cells and prevented flocculation of pahs in an aqueous suspension. a w ... | 1996 | 8776849 |
| physicochemical properties of pm-factor, a surface-active agent produced by pseudomonas marginalis. | an extracellular surface-active agent, pm-factor, was obtained by high-speed centrifugation from the culture broth of pseudomonas marginalis pd-14b. pm-factor exhibited emulsifying activity on a broad spectrum of hydrocarbon liquids, including aromatics, aliphatics, crude oil, and creosote. the factor appeared as ball-shaped particles of varying diameter when examined by electron microscopy (0.16-1.4 microns). gel filtration chromatography demonstrated a high molecular mass of the factor (> 10(6 ... | 1996 | 8868231 |
| nmr analysis of galactoglucan from pseudomonas marginalis: assignment of the 1h and 13c nmr spectra and location of succinate groups. | | 1996 | 8901271 |
| the azotobacter vinelandii gene algj encodes an outer-membrane protein presumably involved in export of alginate. | the algj gene from azotobacter vinelandii was cloned using a labelled rna probe representing the coding region of the alge gene from pseudomonas aeruginosa. dna sequencing revealed an orf of 1452 bp encoding a protein of 484 amino acid residues with a calculated molecular mass of 54611 da. an rna probe corresponding to alge was also used for southern hybridization of chromosomal dna, which showed that alge-related dna sequences are also present in the alginate-producing phytopathogen species pse ... | 1996 | 8936313 |
| identification of gene loci controlling pectate lyase production and soft-rot pathogenicity in pseudomonas marginalis. | pseudomonas marginalis is an important postharvest pathogen capable of causing soft rot in a wide variety of harvested fruits and vegetables. following transposon mutagenesis, we isolated two groups of p. marginalis cy091 mutants deficient in production of pectate lyase (pel) and soft-rot pathogenicity in plants. the first group, designated pel-, was caused by the insertion of tn5 into a pel structural gene, and the second group, designated lema-, was caused by the insertion of tn5 into a regula ... | 1997 | 9165701 |
| pectinolytic enzymes from pseudomonas marginalis maff 03-01173. | two pectinolytic enzymes were purified from the culture broth of pseudomonas marginalis pv. marginalis maff 03-01173 with total 33% recovery of the initial activity. from the substrate specificities against pectin and polygalacturonic acid, the requirement of calcium ion for the enzymatic activity, and the n-terminal sequences, the enzymes were identified as pectin lyase and pectate lyase. the m,s of pectin lyase and pectate lyase were estimated to be 34,000 and 43,000, respectively, by sds poly ... | 1997 | 9237399 |
| biochemical and genetic characterization of an extracellular protease from pseudomonas fluorescens cy091. | pseudomonas fluorescens cy091 cultures produce an extracellular protease with an estimated molecular mass of 50 kda. production of this enzyme (designated aprx) was observed in media containing cacl2 or srcl2 but not in media containing zncl2, mgcl2, or mncl2. the requirement of ca2+ (or sr2+) for enzyme production was concentration dependent, and the optimal concentration for production was determined to be 0.35 mm. following ammonium sulfate precipitation and ion-exchange chromatography, the a ... | 1998 | 9501431 |
| the rhizobium meliloti exok and exsh glycanases specifically depolymerize nascent succinoglycan chains. | the rhizobium meliloti exok and exsh glycanases have been proposed to contribute to production of low molecular weight (lmw) succinoglycan by depolymerizing high molecular weight succinoglycan chains in r. meliloti cultures. we expressed and purified exok and exsh and determined that neither enzyme can extensively cleave succinoglycan prepared from r. meliloti cultures, although neutral/heat treatment and acid/heat treatment convert succinoglycan to forms that can be cleaved efficiently by both ... | 1998 | 9560202 |
| detection of ralstonia solanacearum, which causes brown rot of potato, by fluorescent in situ hybridization with 23s rrna-targeted probes. | during the past few years, ralstonia (pseudomonas) solanacearum race 3, biovar 2, was repeatedly found in potatoes in western europe. to detect this bacterium in potato tissue samples, we developed a method based on fluorescent in situ hybridization (fish). the nearly complete genes encoding 23s rrna of five r. solanacearum strains and one ralstonia pickettii strain were pcr amplified, sequenced, and analyzed by sequence alignment. this resulted in the construction of an unrooted tree and suppor ... | 1998 | 9797321 |
| azospirillum irakense produces a novel type of pectate lyase. | the pela gene from the n2-fixing plant-associated bacterium azospirillum irakense, encoding a pectate lyase, was isolated by heterologous expression in escherichia coli. nucleotide sequence analysis of the region containing pela indicated an open reading frame of 1,296 bp, coding for a preprotein of 432 amino acids with a typical amino-terminal signal peptide of 24 amino acids. n-terminal amino acid sequencing confirmed the processing of the protein in e. coli at the signal peptidase cleavage si ... | 1999 | 10198006 |
| lead resistance in two bacterial isolates from heavy metal-contaminated soils. | > abstract microorganisms have developed mechanisms of coping with a variety of toxic metals; however, few studies have explored microbial resistance to lead. in this study, the overall mechanisms of a lead-resistant pseudomonas marginalis and a lead-resistant bacillus megaterium isolated from two different metal-contaminated soils were investigated. the p.marginalis had a higher lead resistance level at 2.5mm total lead as compared to 0.6 mm for b. megaterium. resistance to soluble lead was muc ... | 1999 | 10227879 |
| enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier alasan. | alasan, a high-molecular-weight bioemulsifier complex of an anionic polysaccharide and proteins that is produced by acinetobacter radioresistens ka53 (s. navon-venezia, z. zosim, a. gottlieb, r. legmann, s. carmeli, e. z. ron, and e. rosenberg, appl. environ. microbiol. 61:3240-3244, 1995), enhanced the aqueous solubility and biodegradation rates of polyaromatic hydrocarbons (pahs). in the presence of 500 microg of alasan ml-1, the apparent aqueous solubilities of phenanthrene, fluoranthene, and ... | 1999 | 10347063 |
| pseudomonas libanensis sp. nov., a new species isolated from lebanese spring waters. | the taxonomic position of eight fluorescent pseudomonas isolates, from two lebanese spring waters, which were previously recognized by numerical analysis as members of a new subcluster (subcluster vb) was examined. except for one strain, the new subcluster exhibited internal dna hybridization values of 76-100%, and 9-53% hybridization was measured with the type or reference strains of other pseudomonas species. the highest dna binding value was found with pseudomonas marginalis strains (37-53%). ... | 1999 | 10425766 |
| immunochemical characterization and taxonomic evaluation of the o polysaccharides of the lipopolysaccharides of pseudomonas syringae serogroup o1 strains. | the o polysaccharide (ops) of the lipopolysaccharide (lps) of pseudomonas syringae pv. atrofaciens imv 7836 and some other strains that are classified in serogroup o1 was shown to be a novel linear alpha-d-rhamnan with the tetrasaccharide o repeat -->3)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->2)-alpha-d-r hap-(1-->2)- alpha-d-rhap-(1--> (chemotype 1a). the same alpha-d-rhamnan serves as the backbone in branched opss with lateral (alpha1-->3)-linked d-rhap, (beta1-->4)-linked d-glcpnac, and (alpha ... | 1999 | 10559159 |
| production of pectate lyases and cellulases by chryseomonas luteola strain mfcl0 depends on the growth temperature and the nature of the culture medium: evidence for two critical temperatures. | several extracellular enzymes that are responsible for plant tissue maceration were detected in culture supernatant of the psychrotrophic bacterium chryseomonas luteola mfcl0. isoelectrofocusing experiments showed that pectate lyase (pl) activity resulted from the cumulative action of three major isoenzymes, designated pli, plii, and pliii. cellulolytic activity was also detected in culture supernatants. these enzymes exhibited different behaviors with respect to growth temperature. plii was not ... | 2000 | 10742239 |
| methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. | miniaturized growth systems for heterogeneous culture collections are not only attractive in reducing demands for incubation space and medium but also in making the parallel handling of large numbers of strains more practicable. we report here on the optimization of oxygen transfer rates in deep-well microtiter plates and the development of a replication system allowing the simultaneous and reproducible sampling of 96 frozen glycerol stock cultures while the remaining culture volume remains froz ... | 2000 | 10831450 |
| detection of pseudomonas savastanoi pv. savastanoi in olive plants by enrichment and pcr. | the sequence of the gene iaal of pseudomonas savastanoi ew2009 was used to design primers for pcr amplification. the iaal-derived primers directed the amplification of a 454-bp fragment from genomic dna isolated from 70 strains of p. savastanoi, whereas genomic dna from 93 non-p. savastanoi isolates did not yield this amplified product. a previous bacterial enrichment in the semiselective liquid medium pvf-1 improved the pcr sensitivity level, allowing detection of 10 to 100 cfu/ml of plant extr ... | 2000 | 10831456 |
| detection of ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic pcr (taqman) assay. | a fluorogenic (taqman) pcr assay was developed to detect ralstonia solanacearum strains. two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (rs) detected all biovars of r. solanacearum, and a second more specific probe (b2) detected only biovar 2a. amplification of the target was measured by the 5' nuclease activity of taq dna polymerase on each probe, resulting in emission of fluorescence. taqman pcr was performed with dna extracted from 42 r. solanacearum and g ... | 2000 | 10877778 |
| in vitro antimicrobial properties of recombinant asabf, an antimicrobial peptide isolated from the nematode ascaris suum. | asabf is a csalphabeta-type antimicrobial peptide that contains four intramolecular disulfide bridges (y. kato and s. komatsu, j. biol. chem. 271:30493-30498, 1996). in the present study, a recombinant asabf was produced by using a yeast expression system, and its antimicrobial activity was characterized in detail. the recombinant asabf was active against all gram-positive bacteria tested (7 of 7; minimum bactericidal concentration [mbc], 0.03 to 1 microg/ml) except leuconostoc mesenteroides, so ... | 2000 | 10991847 |
| sira orthologs affect both motility and virulence. | the sira gene of salmonella enterica serovar typhimurium encodes a two-component response regulator of the fixj family that has a positive regulatory influence on the expression of type iii secretion genes involved with epithelial cell invasion and the elicitation of bovine gastroenteritis. sira orthologs in pseudomonas, vibrio, and erwinia control the expression of distinct virulence genes in these genera, but an evolutionarily conserved target of sira regulation has never been identified. in t ... | 2001 | 11244064 |
| effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rrna gene in pcr-based microbial community analyses and genetic profiling. | genetic profiling techniques of microbial communities based on pcr-amplified signature genes, such as denaturing gradient gel electrophoresis or single-strand-conformation polymorphism (sscp) analysis, are normally done with pcr products of less than 500-bp. the most common target for diversity analysis, the small-subunit rrna genes, however, are larger, and thus, only partial sequences can be analyzed. here, we compared the results obtained by pcr targeting different variable (v) regions (v2 an ... | 2001 | 11472932 |
| low-temperature lipase from psychrotrophic pseudomonas sp. strain kb700a. | we have previously reported that a psychrotrophic bacterium, pseudomonas sp. strain kb700a, which displays sigmoidal growth even at -5 degrees c, produced a lipase. a genomic dna library of strain kb700a was introduced into escherichia coli tg1, and screening on tributyrin-containing agar plates led to the isolation of the lipase gene. sequence analysis revealed an open reading frame (kb-lip) consisting of 1,422 nucleotides that encoded a protein (kb-lip) of 474 amino acids with a molecular mass ... | 2001 | 11526006 |
| cloning and functional analysis of the pbr lead resistance determinant of ralstonia metallidurans ch34. | the lead resistance operon, pbr, of ralstonia metallidurans (formerly alcaligenes eutrophus) strain ch34 is unique, as it combines functions involved in uptake, efflux, and accumulation of pb(ii). the pbr lead resistance locus contains the following structural resistance genes: (i) pbrt, which encodes a pb(ii) uptake protein; (ii) pbra, which encodes a p-type pb(ii) efflux atpase; (iii) pbrb, which encodes a predicted integral membrane protein of unknown function; and (iv) pbrc, which encodes a ... | 2001 | 11544228 |
| characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase. | fifteen bacterial strains containing 1-aminocyclopropane-1-carboxylate (acc) deaminase were isolated from the rhizoplane of pea (pisum sativum l.) and indian mustard (brassica juncea l.) grown in different soils and a long-standing sewage sludge contaminated with heavy metals. the isolated strains were characterized and assigned to various genera and species, such as pseudomonas brassicacearum, pseudomonas marginalis, pseudomonas oryzihabitans, pseudomonas putida, pseudomonas sp., alcaligenes xy ... | 2001 | 11547884 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| pseudomonas grimontii sp. nov. | the vernacular name 'fluorescent pseudomonas group 97-514' was coined for a group of 43 strains isolated from two french natural mineral waters. all these strains were gram-negative, rod-shaped and motile by means of a single polar flagellum. they produced fluorescent pigment (pyoverdin) on king b medium, catalase and cytochrome oxidase. they were capable of respiratory but not fermentative metabolism. they were not able to accumulate poly-beta-hydroxybutyrate and possessed an arginine dihydrola ... | 2002 | 12361251 |
| plant lectin-like bacteriocin from a rhizosphere-colonizing pseudomonas isolate. | rhizosphere isolate pseudomonas sp. strain bw11m1, which belongs to the pseudomonas putida cluster, secretes a heat- and protease-sensitive bacteriocin which kills p. putida gr12-2r3. the production of this bacteriocin is enhanced by dna-damaging treatment of producer cells. we isolated a tnmod mutant of strain bw11m1 that had lost the capacity to inhibit the growth of strain gr12-2r3. a wild-type genomic fragment encompassing the transposon insertion site was shown to confer the bacteriocin phe ... | 2003 | 12533465 |
| high-performance liquid chromatography analyses of pyoverdin siderophores differentiate among phytopathogenic fluorescent pseudomonas species. | the relationship of pyoverdins produced by 41 pathovars of pseudomonas syringae and by phytopathogenic pseudomonas species was investigated. a high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical fe(iii)-chelated pyoverdins. nonfluorescent strains were found in pseudomonas amygdali, pseudomonas ... | 2003 | 12571041 |
| overexpression of the pectin lyase gene of pseudomonas marginalis in escherichia coli and purification of the active enzyme. | a pectin lyase gene (pnl) of pseudomonas marginalis was cloned and overexpressed in escherichia coli bl21(de3). the pnl gene was amplified by pcr, inserted into pet29c with a six-his tag and the overproduced active enzyme was purified almost to homogeneity using a ni(2+)-nitrilotriacetate-agarose column. the purified pectin lyase (pnl; ec 4.2.2.10, family 1) is inhibited by nad+ (at concentrations above 0.25 mm), nadh or dithiothreitol. evidence for the existence of a heat-labile protein inhibit ... | 2003 | 12630908 |
| microbial population diversity in the urethras of healthy males and males suffering from nonchlamydial, nongonococcal urethritis. | nonchlamydial, nongonococcal urethritis (ncngu) is suggested to be a sexually transmitted disease in men. ncngu patients were compared to control subjects with regard to the presence of potentially infectious bacteria in the first void urine. patients' pre- and post-antibiotic-treatment urine samples and two samples obtained 2 weeks apart from healthy volunteers, who did not receive antibiotic therapy, were analyzed with broad-spectrum pcr tests aiming at eubacterial small subunit rrna genes. re ... | 2003 | 12734237 |
| microbial consortia that degrade 2,4-dnt by interspecies metabolism: isolation and characterisation. | two consortia, isolated by selective enrichment from a soil sample of a nitroaromatic-contaminated site, degraded 2,4-dnt as their sole nitrogen source without accumulating one or more detectable intermediates. though originating from the same sample, the optimised consortia had no common members, indicating that selective enrichment resulted in different end points. consortium 1 and consortium 2 contained four and six bacterial species respectively, but both had two members that were able to co ... | 2003 | 12801097 |
| effect of ultraviolet light on pectolytic enzyme production and pathogenicity of pseudomonas. | ultraviolet radiation-induced mutants of the soft rot bacterium pseudomonas marginalis were selected for loss of pathogenicity for lettuce and witloof chicory. the avirulent mutants differed from the parent pathogen in their inability to synthesize pectolytic enzymes in culture or to ferment sodium pectate or sodium polygalacturonate as the sole carbon source in media. | 1959 | 13635008 |
| comparison of application methods to prolong the survival of potential biocontrol bacteria on stored sugar-beet seed. | to develop bacterial inoculation treatments on sugar-beet seed that will maintain a commercially acceptable degree of viability for a minimum of 4 months storage at ambient temperature. | 2004 | 15239695 |
| comparison of atpase-encoding type iii secretion system hrcn genes in biocontrol fluorescent pseudomonads and in phytopathogenic proteobacteria. | type iii protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. certain type iii protein secretion genes (e.g., hrcc) have been found in pseudomonas sp. strain sbw25 (and other biocontrol pseudomonads), but other type iii protein secretion genes, such as the atpase-encoding gene hrcn, have not been found. using both colony hybridization and a pcr approach, we show here that hrcn is nevertheles ... | 2004 | 15345390 |
| characterization and performance of a toluene-degrading biofilm developed on pumice stones. | background: hydrocarbon-degrading biofilms in the treatment of contaminated groundwaters have received increasing attention due to the role played in the so-called "biobarriers". these are bioremediation systems in which a microbial consortium adherent to a solid support is placed across the flow of a contaminated plume, thus promoting biodegradation of the pollutant. results: a microbial consortium adherent to pumice granules (biofilm) developed from a toluene-enriched microflora in a mini-scal ... | 2005 | 15655073 |
| production of rhamnolipids by pseudomonas chlororaphis, a nonpathogenic bacterium. | rhamnolipids, naturally occurring biosurfactants constructed of rhamnose sugar molecules and beta-hydroxyalkanoic acids, have a wide range of potential commercial applications. in the course of a survey of 33 different bacterial isolates, we have identified, using a phenotypic assay for rhamnolipid production, a strain of the nonpathogenic bacterial species pseudomonas chlororaphis that is capable of producing rhamnolipids. rhamnolipid production by p. chlororaphis was achieved by growth at room ... | 2005 | 15870313 |
| evidence that dl-3-aminobutyric acid and acibenzolar-s-methyl induce resistance against bacterial head rot disease of broccoli. | head rot of broccoli caused by pseudomonas marginalis (brown) stevens and p. fluorescens migula is a major disease in brittany (france). to date, no accession with a satisfactory field resistance has been identified, and available pesticides are not effective in controlling the disease. the aim of this study was to test whether acibenzolar-s-methyl (asm), dl-3-aminobutyric acid (baba) and potassium phosphonate (k(2)hpo(3)), known to induce resistance against various diseases, can help protect br ... | 2005 | 16041690 |
| nppdr1, a pleiotropic drug resistance-type atp-binding cassette transporter from nicotiana plumbaginifolia, plays a major role in plant pathogen defense. | nicotiana plumbaginifolia nppdr1, a plasma membrane pleiotropic drug resistance-type atp-binding cassette transporter formerly named npabc1, has been suggested to transport the diterpene sclareol, an antifungal compound. however, direct evidence for a role of pleiotropic drug resistance transporters in the plant defense is still lacking. in situ immunolocalization and histochemical analysis using the gusa reporter gene showed that nppdr1 was constitutively expressed in the whole root, in the lea ... | 2005 | 16126865 |
| isolation and characterization of psychrotrophs from subterranean environments. | subterranean environments are potential sources for the isolation of novel microorganisms. water and soil samples were collected at depths ranging from 10 to 1800 meters below the surface, and screening was carried out with aerobic rich and anaerobic minimal media. two psychrotrophic and three chemoautotrophic strains were isolated. one of the psychrotrophic isolates, designated sn16a, grew at temperatures between -5 and 37 degrees c with optimal growth between 25 and 30 degrees c. the other psy ... | 1999 | 16232548 |
| evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes. | pcr was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (acc) deaminase genes from bacteria. the shimodaira-hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (hgt) were significantly favored over vertical transmission. the best maximum likelihood (ml) acc deaminase tree was significantly more likely than four alternative ml trees, suggesting hgt. | 2005 | 16269802 |
| bacterial growth rates and competition affect nodulation and root colonization by rhizobium meliloti. | the addition of streptomycin to nonsterile soil suppressed the numbers of bacterial cells in the rhizosphere of alfalfa (medicago sativa l.) for several days, resulted in the enhanced growth of a streptomycin-resistant strain of rhizobium meliloti, and increased the numbers of nodules on the alfalfa roots. a bacterial mixture inoculated into sterile soil inhibited the colonization of alfalfa roots by r. meliloti, caused a diminution in the number of nodules, and reduced plant growth. enterobacte ... | 1986 | 16347173 |
| effects of temperature, ph, and nacl on growth and pectinolytic activity of pseudomonas marginalis. | the interaction of temperature (4, 10, 18, and 30 degrees c), ph (6, 7, and 8), and nacl (0, 2.5, and 5%) and their effects on specific growth rate, lag phase, and pectinolytic enzymes of pseudomonas marginalis were evaluated. response surface methodology was adapted to describe the response of growth parameters to environmental changes. to obtain good conditions of storage, the combined action of salt and temperature is necessary. at 4 degrees c with an nacl concentration of 5% and a ph of 7, t ... | 1994 | 16349288 |
| encapsulated escherichia coli in alginate beads capable of secreting a heterologous pectin lyase. | production of heterologous proteins in the e. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. the aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently secreted and the encapsulation of transformed e. coli cells for pectin degradation in a biotechnological process. | 2005 | 16354300 |
| bacterial endophytes of the wildflower crocus albiflorus analyzed by characterization of isolates and by a cultivation-independent approach. | the presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (crocus albiflorus), a wildflower native in the alps, were investigated. a combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16s rdna pcr amplification followed by whole-community fingerprinting (t-rflp) and by construction of a bacterial clone library was used. the results clearly indicated that a wide range of bacteria from diverse phylogenetic affiliatio ... | 2006 | 16541150 |
| comparison of two fingerprinting techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments. | we investigated bacterial diversity in different aquatic environments (including marine and lagoon sediments, coastal seawater, and groundwater), and we compared two fingerprinting techniques (terminal restriction fragment length polymorphism [t-rflp] and automated ribosomal intergenic spacer analysis [arisa]) which are currently utilized for estimating richness and community composition. bacterial diversity ranged from 27 to 99 phylotypes (on average, 56) using the t-rflp approach and from 62 t ... | 2006 | 16957219 |
| biofilm formation and cellulose expression among diverse environmental pseudomonas isolates. | the ability to form biofilms is seen as an increasingly important colonization strategy among both pathogenic and environmental bacteria. a survey of 185 plant-associated, phytopathogenic, soil and river pseudomonas isolates resulted in 76% producing biofilms at the air-liquid (a-l) interface after selection in static microcosms. considerable variation in biofilm phenotype was observed, including waxy aggregations, viscous and floccular masses, and physically cohesive biofilms with continuously ... | 2006 | 17014498 |
| biofilm formation and biocides sensitivity of pseudomonas marginalis isolated from a maple sap collection system. | the susceptibility of planktonic and biofilm cells of pseudomonas marginalis toward four commonly used biocides at different temperatures (15 and 30 degrees c) and biofilm growth times (24 and 48 h) was assessed. using the mbec biofilm device, biofilm production in maple sap was shown to be highly reproducible for each set of conditions tested. biofilm formation was influenced by growth temperature and time. a temperature of 15 degrees c and incubation time of 24 h yielded fewer cfu per peg and ... | 2006 | 17066920 |
| isolation and assessment of phytate-hydrolysing bacteria from the delmarva peninsula. | the delaware-maryland-virginia (delmarva) peninsula, flanking one side of the chesapeake bay, is home to a substantial broiler chicken industry. as such, it produces a significant amount of manure that is typically composted and spread onto local croplands as a fertilizer. phytate (myo inositol hexakisphosphate), the major form of organic phosphorus in the manure, can be hydrolysed by microorganisms to produce orthophosphate. orthophosphate is a eutrophication agent which can lead to algal bloom ... | 2007 | 17991037 |
| inhibition of foodborne pathogens by native microflora recovered from fresh peeled baby carrot and propagated in cultures. | the inhibitory effect of fresh peeled baby carrot and associated microflora on the viability and growth of listeria monocytogenes, yersinia enterocolitica, salmonella enterica, escherichia coli o157:h7, and pseudomonas marginalis was investigated. the population of viable l. monocytogenes was reduced by more than 2 logs following the dipping of sliced baby carrots in the bacterial suspension for 2 min. however, other pathogens subjected to the same treatment showed no reduction in the population ... | 2007 | 17995781 |
| control of foodborne pathogens and soft-rot bacteria on bell pepper by three strains of bacterial antagonists. | forty-two representative strains of native bacteria associated with fresh peeled baby carrots were isolated and characterized. two of these strains, identified as pseudomonas fluorescens ag3a (pf ag3a) and bacillus yd1, were evaluated in conjunction with another known antagonist, p. fluorescens 2-79 (pf 2-79), for their potential as biocontrol agents of human pathogens (listeria monocytogenes, yersinia enterocolitica, salmonella enterica, and escherichia coli o157:h7) and soft-rot bacteria (erwi ... | 2009 | 19205468 |
| interactions among selected endoparasitic nematodes and three pseudomonads on alfalfa. | meloidogyne hapla, pratylenchus penetrans, and helicotylenchus dihystera, reduced the growth of 'saranac ar alfalfa seedlings when applied at concentrations of 50 nematodes per plant. all except p. penetrans reduced seedling growth when applied at 25 per seedling. m. hapla reduced growth when applied at 12 per seedling. nematodes interacted with three pseudomonads to produce greater growth reductions than were obtained with single pathogens, suggesting synergistic relationships. ditylenchus dips ... | 1982 | 19295682 |
| [phenotypical identification of wet rot pathogen in lupine]. | a comparative analysis of pathogenic, cultural, physiological-biochemical properties and cell antigenic and fatty acids composition of 13 pseudomonas xanthochlora strains with collection strains of bacteria which belong to pseudomonas genus and strike legumes has been determined. the significant affinity between agent of lupin's bacterial wet rot and representatives of pseudomonas marginalis species by a complex phenotypical properties has been shown. the paper is presented in ukrainian. | 2010 | 20455441 |
| structure, mechanism, and substrate specificity of kynureninase. | the kynurenine pathway is the major route for tryptophan catabolism in animals and some fungi and bacteria. the procaryotic enzyme preferentially reacts with l-kynurenine, while eucaryotic kynureninases exhibit higher activity with 3-hydroxy-l-kynurenine. crystallography of kynureninases from pseudomonas fluorescens (pfkyn) and homo sapiens (hskyn) shows that the active sites are nearly identical, except that his-102, asn-333, and ser-332 in hskyn are replaced by trp-64, thr-282, and gly-281 in ... | 2010 | 21167323 |
| structure, mechanism, and substrate specificity of kynureninase. | the kynurenine pathway is the major route for tryptophan catabolism in animals and some fungi and bacteria. the procaryotic enzyme preferentially reacts with l-kynurenine, while eucaryotic kynureninases exhibit higher activity with 3-hydroxy-l-kynurenine. crystallography of kynureninases from pseudomonas fluorescens (pfkyn) and homo sapiens (hskyn) shows that the active sites are nearly identical, except that his-102, asn-333, and ser-332 in hskyn are replaced by trp-64, thr-282, and gly-281 in ... | 2010 | 21167323 |
| enzymes hydrolyzing structural components and ferrous ion cause rusty-root symptom on ginseng (panax ginseng). | microbial induction of rusty-root was proved in this study. the enzymes hydrolyzing plant structural materials, including pectinase, pectolyase, ligninase, and cellulase, caused the rusty-root in ginseng. pectinase and pectolyase produced the highest rusty-color formation. ferrous ion (fe+++) caused the synergistic effect on rusty-root formation in ginseng when it was used with pectinase. the effect of ferric ion (fe++) on rusty-root formation was slow, compared with fe+++, probably due to gradu ... | 2011 | 21364303 |
| [phylogenetic analysis of lupine's bacterial wet rot--"pseudomonas xanthochlora"]. | the sequencing of 16s rrna gene nucleotide chain of the 12 "p. xanthochlora" strains, collection pseudomonas marginalis 8572 strain and pseudomonas marginalis pv. marginalis 9175t p. fluorescens b-17t typical strains has been determined. the analysis of the 16s rrna gene nucleotide chain showed high level of homology (98-99%) of "p. xanthochlora" investigated strains with the same of representatives of both p. fluorescens and p. marginalis species. | 2011 | 22308747 |
| in silico characterization of pectate lyase protein sequences from different source organisms. | a total of 121 protein sequences of pectate lyases were subjected to homology search, multiple sequence alignment, phylogenetic tree construction, and motif analysis. the phylogenetic tree constructed revealed different clusters based on different source organisms representing bacterial, fungal, plant, and nematode pectate lyases. the multiple accessions of bacterial, fungal, nematode, and plant pectate lyase protein sequences were placed closely revealing a sequence level similarity. the multip ... | 2010 | 21048874 |
| a persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations. | acute exacerbations of chronic obstructive pulmonary disease (copd) are a major source of morbidity and contribute significantly to healthcare costs. although bacterial infections are implicated in nearly 50% of exacerbations, only a handful of pathogens have been consistently identified in copd airways, primarily by culture-based methods, and the bacterial microbiota in acute exacerbations remains largely uncharacterized. the aim of this study was to comprehensively profile airway bacterial com ... | 2010 | 20141328 |
| pgprs and nitrogen-fixing legumes: a perfect team for efficient cd phytoremediation? | cadmium (cd) is a toxic, biologically non-essential and highly mobile metal that has become an increasingly important environmental hazard to both wildlife and humans. in contrast to conventional remediation technologies, phytoremediation based on legume-rhizobia symbiosis has emerged as an inexpensive decontamination alternative which also revitalize contaminated soils due to the role of legumes in nitrogen cycling. in recent years, there is a growing interest in understanding symbiotic legume- ... | 2015 | 25763004 |
| the metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs. | the exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. this study aimed to investigate whether metabolically active bacteria (mab) could be detected within tonsils and mandibular lymph nodes (mlns) of pigs. the hypervariable v1-v2 region of the bacterial 16s rrna genes was amplified from cdna from tonsils and mlns of eight clinically healthy slaughter p ... | 2015 | 26696976 |
| simultaneous detection of major blackleg and soft rot bacterial pathogens in potato by multiplex polymerase chain reaction. | a multiplex polymerase chain reaction (pcr) assay for simultaneous, fast and reliable detection of the main soft rot and blackleg potato pathogens in europe has been developed. it utilises three pairs of primers and enables detection of three groups of pectinolytic bacteria frequently found in potato, namely: pectobacterium atrosepticum, pectobacterium carotovorum subsp. carotovorum together with pectobacterium wasabiae and dickeya spp. in a multiplex pcr assay. in studies with axenic cultures o ... | 2014 | 25506085 |
| native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses. | all plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth. changes in plant growth frequently reflect alterations in phytohormone homoeostasis by plant-growth-promoting (pgp) rhizobacteria which can decrease ethylene (et) levels enzymatically by 1-aminocyclopropane-1-carboxylate (acc) deaminase or produce indole acetic acid (iaa). whether these common pgp mechanisms work similarly for different plant species has not been rigorously teste ... | 2008 | 18628963 |
| pseudomonas fluorescens sbw25 produces furanomycin, a non-proteinogenic amino acid with selective antimicrobial properties. | pseudomonas fluorescens sbw25 has been extensively studied because of its plant growth promoting properties and potential as a biocontrol agent. the genome of sbw25 has been sequenced, and among sequenced strains of pseudomonads, sbw25 appears to be most closely related to p. fluorescens wh6. in the authors' laboratories, wh6 was previously shown to produce and secrete 4-formylaminooxyvinylglycine (fvg), a non-proteinogenic amino acid with selective herbicidal and antimicrobial activity. althoug ... | 2013 | 23688329 |
| the two-component regulators gacs and gaca positively regulate a nonfluorescent siderophore through the gac/rsm signaling cascade in high-siderophore-yielding pseudomonas sp. strain hys. | siderophores, which are produced to overcome iron deficiency, are believed to be closely related to the adaptability of bacteria. the high-siderophore-yielding pseudomonas sp. strain hys simultaneously secretes the fluorescent siderophore pyoverdine and another nonfluorescent siderophore that is a major contributor to the high siderophore yield. transposon mutagenesis revealed siderophore-related genes, including the two-component regulators gacs/gaca and a special cluster containing four open r ... | 2014 | 24982309 |
| classification and taxonomy of vegetable macergens. | macergens are bacteria capable of releasing pectic enzymes (pectolytic bacteria). these enzymatic actions result in the separation of plant tissues leading to total plant destruction. this can be attributed to soft rot diseases in vegetables. these macergens primarily belong to the genus erwinia and to a range of opportunistic pathogens namely: the xanthomonas spp., pseudomonas spp., clostridium spp., cytophaga spp., and bacillus spp. they consist of taxa that displayed considerable heterogeneit ... | 2015 | 26640465 |
| different ancestries of r tailocins in rhizospheric pseudomonas isolates. | bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated dna. a subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. functional characterization of bactericidal tailocins of two pseudomonas pu ... | 2015 | 26412856 |
| pseudomonas aeruginosa type iii secretory toxin exou and its predicted homologs. | pseudomonas aeruginosa exou, a type iii secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. through use of a recently updated bacterial genome database, protein sequences predicted to be homologous to ps. aeruginosa exou were identified in 17 other pseudomonas species (ps. fluorescens, ps. lundensis, ps. weihenstephanensis, ps. marginalis, ps. rhodesiae, ps. synxantha, ps. libanensis, ... | 2016 | 27792159 |
| high quality draft genome sequence of the type strain of pseudomonas lutea ok2(t), a phosphate-solubilizing rhizospheric bacterium. | pseudomonas lutea ok2(t) (=lmg 21974(t), cect 5822(t)) is the type strain of the species and was isolated from the rhizosphere of grass growing in spain in 2003 based on its phosphate-solubilizing capacity. in order to identify the functional significance of phosphate solubilization in pseudomonas plant growth promoting rhizobacteria, we describe here the phenotypic characteristics of strain ok2(t) along with its high-quality draft genome sequence, its annotation, and analysis. the genome is com ... | 2016 | 27555890 |
| pseudomonas lini strain zbg1 revealed carboxylic acid utilization and copper resistance features required for adaptation to vineyard soil environment: a draft genome analysis. | pseudomonas lini strain zbg1 was isolated from the soil of vineyard in zellenberg, france and the draft genome was reported in this study. bioinformatics analyses of the genome revealed presence of genes encoding tartaric and malic acid utilization as well as copper resistance that correspond to the adaptation this strain in vineyard soil environment. | 2016 | 27512520 |
| biosurfactants: multifunctional biomolecules of the 21st century. | in the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. such characteristics allow these biomolecules to play a key r ... | 2016 | 26999123 |
| development of a sensitive and specific polyclonal antibody for serological detection of clavibacter michiganensis subsp. sepedonicus. | the quarantine bacterium clavibacter michiganensis subsp. sepedonicus (cms) causes bacterial ring rot (brr) in potato but is difficult to detect, hampering the diagnosis of this disease. elisa immunoassays have not been widely used to detect cms because commercially available anti-cms antibodies detect mainly eps-producing bacteria and can fail to detect strains that do not produce eps. in the current study, we developed a new type of polyclonal antibody that specifically detects clavibacter mic ... | 2017 | 28068400 |
| monitoring of microbial hydrocarbon remediation in the soil. | bioremediation of hydrocarbon pollutants is advantageous owing to the cost-effectiveness of the technology and the ubiquity of hydrocarbon-degrading microorganisms in the soil. soil microbial diversity is affected by hydrocarbon perturbation, thus selective enrichment of hydrocarbon utilizers occurs. hydrocarbons interact with the soil matrix and soil microorganisms determining the fate of the contaminants relative to their chemical nature and microbial degradative capabilities, respectively. pr ... | 2011 | 22611524 |
| sura is involved in the targeting to the outer membrane of a tat signal sequence-anchored protein. | the twin arginine translocation (tat) pathway exports folded proteins from the cytoplasm to the periplasm of bacteria. the targeting of the exported proteins to the tat pathway relies on a specific amino-terminal signal sequence, which is cleaved after exportation. in the phytopathogen dickeya dadantii, the pectin lyase homologue pnlh is exported by the tat pathway without cleavage of its signal sequence, which anchors pnlh into the outer membrane. in proteobacteria, the vast majority of outer m ... | 2012 | 22961852 |
| development of an engineered bioluminescent reporter phage for detection of bacterial blight of crucifers. | bacterial blight, caused by the phytopathogen pseudomonas cannabina pv. alisalensis, is an emerging disease afflicting important members of the brassicaceae family. the disease is often misdiagnosed as pepper spot, a much less severe disease caused by the related pathogen pseudomonas syringae pv. maculicola. we have developed a phage-based diagnostic that can both identify and detect the causative agent of bacterial blight and differentiate the two pathogens. a recombinant "light"-tagged reporte ... | 2012 | 22427491 |
| genetic diversity and ecological evaluation of fluorescent pseudomonads isolated from the leaves and roots of potato plants. | a total of 828 isolates of fluorescent pseudomonads (fps) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in japan, and 16s rrna gene sequences of 776 isolates were successfully determined by direct pcr sequencing. clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (otus) for leaf- and root-associated fps, respectively, and 29 otus were identified in the phytosphere of potato plants ... | 2012 | 22791043 |
| engineering rhizobial bioinoculants: a strategy to improve iron nutrition. | under field conditions, inoculated rhizobial strains are at a survival disadvantage as compared to indigenous strains. in order to out-compete native rhizobia it is not only important to develop strong nodulation efficiency but also increase their competence in the soil and rhizosphere. competitive survival of the inoculated strain may be improved by employing strain selection and by genetic engineering of superior nitrogen fixing strains. iron sufficiency is an important factor determining the ... | 2013 | 24319357 |
| synthesis and biological evaluation of novel alkyl-imidazolyl carbinols and their esters: potent antimycotics. | a novel series of imidazol-5-yl carbinols and their 4-chlorobenzoyl esters has been synthesized by the grignard reaction and subsequent esterification. these compounds were screened for their antimicrobial activities in an agar diffusion assay. the compounds with c10 to c12-alkyl side chains displayed significant antimycotic activity. | 2013 | 24106663 |
| synthesis and antifungal evaluation of novel n-alkyl tetra- and perhydroquinoline derivatives. | a series of novel n-alkyl tetra- and perhydroquinoline derivatives and their hydrochlorides were prepared from tetrahydro- or trans-perhydroquinoline by direct alkylation with alkyl halides and subsequent precipitation with hcl gas. the antimicrobial activity of the resulting amines was evaluated in an agar diffusion assay. the minimal inhibitory concentrations (mic) of the active compounds were determined by the microdilution method. in contrast to the tetrahydroquinolines, the perhydro analogu ... | 2014 | 26839797 |
| synthesis and antifungal evaluation of novel n-alkyl tetra- and perhydroquinoline derivatives. | a series of novel n-alkyl tetra- and perhydroquinoline derivatives and their hydrochlorides were prepared from tetrahydro- or trans-perhydroquinoline by direct alkylation with alkyl halides and subsequent precipitation with hcl gas. the antimicrobial activity of the resulting amines was evaluated in an agar diffusion assay. the minimal inhibitory concentrations (mic) of the active compounds were determined by the microdilution method. in contrast to the tetrahydroquinolines, the perhydro analogu ... | 2014 | 26839797 |
| sensitive detection of xanthomonas oryzae pathovars oryzae and oryzicola by loop-mediated isothermal amplification. | molecular diagnostics for crop diseases can enhance food security by enabling the rapid identification of threatening pathogens and providing critical information for the deployment of disease management strategies. loop-mediated isothermal amplification (lamp) is a pcr-based tool that allows the rapid, highly specific amplification of target dna sequences at a single temperature and is thus ideal for field-level diagnosis of plant diseases. we developed primers highly specific for two globally ... | 2014 | 24837384 |
| chemical composition and antibacterial activity of essential oils of tagetes minuta (asteraceae) against selected plant pathogenic bacteria. | the objective of this study was to determine the chemical composition and antibacterial activity of essential oils (eos) of tagetes minuta against three phytopathogenic bacteria pseudomonas savastanoi pv. phaseolicola, xanthomonas axonopodis pv. phaseoli, and xanthomonas axonopodis pv. manihotis. the essential oils were extracted using steam distillation method in a modified clevenger-type apparatus while antibacterial activity of the eos was evaluated by disc diffusion method. gas chromatograph ... | 2016 | 27721831 |
| molecular characterization of spoilage bacteria as a means to observe the microbiological quality of carrot. | this study characterized the bacteria causing decay of carrots during storage and marketing. spoilage strains were identified by 16s-amplified rdna restriction analysis and intergenic transcribed spacer-pcr-restriction fragment length polymorphism (its-pcr-rflp). genotypic fingerprinting by rflp-pulsed-field gel electrophoresis was used to assess the genetic diversity of the isolates. a total of 252 pseudomonas isolates from carrots were identified and classified into eight separate groups. most ... | 2012 | 22410227 |
| [identification of causative agent of brassica rapa bacterial diseases based on fatty acid composition of cellular lipids]. | the fatty acid composition of cell lipids of 15 strains isolated from the affected plants of rape and five collection strains has been studied. according to the results of chemotaxonomic analysis it has been found that 9 isolated strains are similar to representatives of species pseudomonas marginalis and pseudomonas fluorescens, and 6 - to those of xanthomonas campestris pv. campestris. the authors have established the efficiency of certain methods for the extraction of fatty acids used for the ... | 2013 | 24006784 |