| d-glucose-6-phosphate dehydrogenase (entner-doudoroff enzyme) from pseudomonas fluorescens. purification, properties and regulation. | 1. the existence of two different d-glucose-6-phosphate dehydrogenases in pseudomonas fluorescens has been demonstrated. based on their different specificity and their different metabolic regulation one enzyme is appointed to the entner-doudoroff pathway and the other to the hexose monophosphate pathway. 2. a procedure is described for the isolation of that d-glucose-6-phosphate dehydrogenase which forms part of the entner-doudoroff pathway (entner-doudoroff enzyme). a 950-fold purification was ... | 1975 | 1257 |
| stereochemistry of the hydrogen transfer to nadp catalyzed by d-galactose dehydrogenase from pseudomonas fluorescens. | | 1975 | 1616 |
| isolation and properties of a homogeneous l-asparaginase preparation from pseudomonas fluorescens ag. | highly purified l-asparaginase having a specific activity of 500+/- +/-40 iu./mg protein is isolated from pseudomonas fluorescens ag cells. the purification procedure includes isopropanol fractionation, gel filtration through sephadex g-100, chromatography on hydroxylapatite and deae-cellulose columns. the asparaginase preparation is homogenous on the basis of polyacrylamide gel electrophoresis data. the ph optimum is found to be 8.0-9.0, isoelectric point and molecular weight are 4.5+/-0.05 and ... | 1975 | 2329 |
| pseudomonas fluorescens bacteria as pathogens of mosquito larvae. | | 1975 | 2854 |
| some properties of the pyruvate carboxylase from pseudomonas fluorescens. | the pyruvate carboxylase of pseudonomas fluorescens was purified 160-fold from cells grown on glucose at 20 degrees c. the activity of this purified enzyme was not affected by acetyl-coenzyme a or l-aspartate, but was strongly inhibited by adp, which was competitive towards atp. pyruvate gave a broken double reciprocal plot, from which two apparent km values could be determined, namely 0-08 and 0-21 mm, from the lower and the higher concentration ranges, respectively. the apparent km for hco3 a ... | 1976 | 4579 |
| histidine ammonia-lyase from rat liver. purification, properties, and inhibition by substrate analogues. | histidine ammonia-lyase (ec 4.3.1.3) from rat liver was purified more than 250-fold to near homogeneity. electrophoretic determinations indicated a native molecular weight of approximately 200,000. the enzyme has a ph optimum of approximately ph 8.5. the minimum km for l-histidine was 0.5 mm at ph 9.0. the michaelis constant in the physiological ph range was, however, more than 2.0 mm. d-alpha-hydrazinoimidazolylpropionic acid was found to be a potent competitive inhibitor of liver histidine amm ... | 1976 | 5116 |
| kinetic studies on pantothenase from pseudomonas fluorescens. effects of ph on substrate and inhibitor binding. | the velocity of the pantothenase-catalysed hydrolysis of pantothenate was studied over ph5.5-9, and in the presence of oxalate or oxaloacetate as an inhibitor. the ph-dependence of the reaction can be described by a kinetic equation containing two ionizations of the enzyme, with one ionizable group located at the substrate-binding site, and the other at the inhibitor-binding site. the km value of pantothenase to pantothenate depends on the buffer used, and phosphate tends to give somewhat lower ... | 1976 | 9072 |
| the extracellular protease from pseudomonas fluorescens. | an extracellular protease has been purified from cultures of pseudomonas fluorescens. it is a metalloenzyme with a molecular weight of 37,000 +/- 3,700, able to digest casein, hemoglobin and gelatine. | 1976 | 9311 |
| on the partial reactivation of inactivated pantothenase from pseudomonas fluorescens. | partial reactivation of inactivated pantothenase (pantothenate amidohydrolase, ec 3.5.1.22) from pseudomonas fluorescens was studied. after partial inactivation during storing, pantothenase activity is increased by 10-40% when incubated with, for instance, oxalate, oxaloacetate or pyruvate. reactivation proceedes slowly; with oxaloacetate the stable level of enzyme activity is attained in 20-30 min. the same compounds also cause reactivation of thermally inactivated pantothenase when partial ina ... | 1976 | 10994 |
| sulfhydryl groups of l-asparaginase a from pseudomonas fluorescens ag. | it has been demonstrated that the activity of asparaginase a from ps. fluorescens ag is completely inhibited by 10(-4) m p-chloromercurybenzoate and by 70-85% by zn2+, ca2+ and cu2+ (2.10(-2) m). iodoacetate, iodoacetamide, n-ethylimide of maleic acid and 5,5'-dithiobis-(2-nitrobenzoic acid) do not decrease the enzyme activity. dithiothreitol and beta-mercaptoethanol reactivate the enzyme. l-asparagine, the substrate of asparaginase, protects the enzyme in a large degree against the inhibitory a ... | 1976 | 14736 |
| lysine decarboxylase in pseudomonas aeruginosa. | the research of lysine, ornithine and arginine decarboxylases has been made for 50 strains of fluorescent pseudomonas (p. aeruginosa, p. fluorescens, p. putida). by thin layer chromatography, all the strains of pseudomonas aeruginosa and the fifth of the strains of p. putida had lysine decarboxylase activity at alcaline ph (optimal ph 8) ; pseudomonas fluorescens did not produce this decarboxylase. arginine and ornithine decarboxylase are absent for all the strains of fluorescent pseudomonas. | 1977 | 19133 |
| purification, crystallization and properties of triacylglycerol lipase from pseudomonas fluorescens. | triacylglycerol lipase of pseudomonas fluorescens was purified from the crude enzyme by ammonium sulfate precipitation and chromatographies on sephadex g-75 and deae-cellulose. the crystallization of the lipase was successfully carried out. the purified lipase was demonstrated to be homogenous on disc electrophoresis and its molecular weight was calculated to be 32 000 by gel filtration. the optimum ph for hydrolysis of sesame oil was 7.0. the enzyme was stable up to 40 degrees c under the condi ... | 1977 | 20147 |
| transport of alpha-aminoisobutyrate by cells and membrane vesicles of pseudomonas fluorescens. | the transport of alpha-aminoisobutyrate into pseudomonas fluorescens ncib 8865 and membrane vesicles prepared from this organism has been studied. uptake by cells was mediated by two active transport systems with different apparent km values, while transport into membrane vesicles was mediated by a single component. the effect of inhibitors on the energy-coupling mechanism for alpha-aminoisobutyrate transport in these systems suggests that a membrane potential may play a significant role in supp ... | 1978 | 26404 |
| effect of the redox potential on the growth of aerobic microorganisms. | the effect of redox potential was studied on the growth of the following aerobic microorganisms: candida utilis, bacillus megaterium, pseudomonas fluorescens. the action of oxidizing agents (k3fe(cn)6, kio3, k2cr207 and kmno4) and reducing agents (ascorbic acid, sodium thioglycolate, k4fe (cn)6 and na2s2o3) on the growth rate was investigated. k3fe(cn)6, ascorbic acid, sodium thioglycolate and k4fe(cn)6 were found to be suitable for buffering the eh of the medium. the potential could be shifted ... | 1978 | 30023 |
| [isolation of the enzyme lipase from pseudomonas fluorescens 533-5b and its characterization]. | from the culture liquid of pseudomonas fluorescens 533-5b, lipase (partially purified by sulphate ammonium precipitation and dialysis) was isolated. the following properties of the enzyme were examined: effect of ph and temperature, effect of bile salts, substrate specificity, and stability during storage. the optimal action of the preparation was at 55 degrees c and ph 7.5-8.0. sodium salts of cholic, taurocholic, deoxycholic, and glycocholic acids at concentrations over 0.5%, as a rule, activa ... | 1978 | 34836 |
| kinetic studies on the reaction of p-hydroxybenzoate hydroxylase. agreement of steady state and rapid reaction data. | p-hydroxybenzoate hydroxylase (ec 1.14.13.2) from pseudomonas fluorescens is a nadph-dependent, fad-containing monooxygenase catalyzing the hydroxylation of p-hydroxybenzoate to form 3,4-dihydroxybenzoate in the presence of nadph and molecular oxygen. the mechanism of this three-substrate reaction was investigated in detail at ph 6.6, 4 degrees c, by steady state kinetics, stopped flow spectrophotometry, and equilibrium binding experiments. the initial velocity patterns are consistent with a pin ... | 1979 | 36402 |
| occurrence of thermolabile and regulatory nad-linked glutamate dehydrogenase in pseudomonas fluorescens. | nad-linked glutamate dehydrogeanse [ec 1.4.1.2] was detected together with nadp-linked glutamate dehydrogenase [ec 1.4.1.4] and aspartase [ec 4.3.1.1] in pseudomonas fluorescens cells. the three enzymes were distinctly separated by deae-sephadex column chromatography. the nad-linked enzyme was extremely thermolabile and was rapidly inactivated even at temperatures as low as 35--40 degrees c. the combined addition of nad+ and glutamate, however, effectively stabilized the enzyme. the glutamate sa ... | 1979 | 37248 |
| effect of carbon source during growth on sensitivity of pseudomonas fluorescens to actinomycin d. | sensitivity to actinomycin d(ad) varies in pseudomonas fluorescens cells grown in glucose or succinate minimal salts medium. growth is inhibited in succinate minimal medium by much lower concentrations of ad than in glucose minimal medium. uptake of selected radioactive metabolites is inhibited by ad in cells incubated for 2 h in succinate medium containing ad but glucose-grown cells were not sensitive. edta treatment promotes increased sensitivity to ad in succinate-grown cells but does not alt ... | 1975 | 46773 |
| effects of low temperature on in vivo and in vitro protein synthesis in escherichia coli and pseudomonas fluorescens. | the effects of temperature on protein synthesis by escherichia coli, a mesophile, and pseudomonas fluorescens, a psychotroph, were investigated by using whole-cell and cell extract preparations. after shifts to 5 degrees c, protein was synthesized at a slowly decreasing rate for 1 h by both organisms, after which p. fluorescens synthesized protein at a new rate corresponding to its 5 degrees growth rate, in contrast to e. coli which did not synthesize protein at a measurable rate. in vitro prote ... | 1978 | 96102 |
| [exolipases of some pseudomonas species]. | the production of exolipase was studied in various pseudomonas species, most of which did not possess the lipolytic activity in the conditions of the experiment, whereas in some strains this activity was rather high. the highest activity was displayed by the pseudomonas fluorescens 533 strain and its mutant obtained upon uv irradiation. lipase biosynthesis depended on the composition of a growth medium, the highest lipolytic activity being found on media with a high content of complex organic su ... | 1978 | 96317 |
| evaluation of yield and maintenance coefficients, expressed in carbon units, for pseudomonas fluorescens and p. aeruginosa. | the maximum cell yield (ymc - g biomass carbon per gram substrate carbon) and the rate of maintenance metabolism (mc - g substrate carbon/g biomass carbon per hour) have been determined for substrate limited continuous cultures of pseudomonas fluorescens and p. aeruginosa. the metabolism of the organic substrates was monitored by measuring the cod-removal1) rates at different dilution rates. the advantages of expressing yield and maintenance coefficients in carbon units is discussed. | 1978 | 96619 |
| f'-plasmid transfer from escherichia coli to pseudomonas fluorescens. | various f' plasmids of escherichia coli k-12 could be transferred into mutants of the soil strain 6.2, classified herein as a pseudomonas fluorescens biotype iv. this strain was previously found to receive flac plasmid (n. datta and r.w. hedges, j. gen microbiol. 70:453-460, 1972). ilv, leu, met, arg, and his auxotrophs were complemented by plasmids carrying isofunctional genes; trp mutants were not complemented or were very poorly complemented. the frequency of transfer was 10(-5). subsequent t ... | 1978 | 97267 |
| [multiplication of bdellovibrio bacteriovorus in the cytoplasm of the bacterial host]. | the bacterial parasite bdellovibrio bacteriovorus was studied in the process of its interaction with the host bacterium pseudomonas fluorescens. as has been shown by time-lapse microcinematography, along with the normal growth of b. bacteriovorus in the periplasmatic space of the host bacterium, occasionally (4--5%) the parasite is located in the cytoplasm where the complete stage of its intracellular growth takes place with the release of progeny. | 1978 | 97504 |
| [substrate specificity of lipase from pseudomonas fluorescens]. | substrate specificity of lipase isolated from the culture liquid filtrate of pseudomonas fluorescens bkm-b-1151 was investigated with respect to vegetable oils and animal fats (olive, sunflower, cotton, mustard and soybean oils; beef and hog fats and their glycerides and fatty acid esters). the preparation showed a high specificity to the quantitative composition of the reaction mixture (substrate: enzyme ratio), chemical structure of the substrate, and the emulgator type (gelatine, gum arabic a ... | 1978 | 97652 |
| purification of pantoate and dimethylmalate dehydrogenase from pseudomonas fluorescens uk-1. | pantoate dehydrogenase and dimethylmalate dehydrogenase were purified 69- and 112-fold, respectively, from pseudomonas fluorescens uk-1 by ammonimu sulphate precipitation. ultrogel aca 34 gel filtration, hydroxyapatite column chromatography, heat treatment and ultrogel aca 44 gel filtration. the enzymes were evaluated for homogeneity (pantoate dehydrogenase was estimated to be about 95% pure) by disc and sodium dodecyl sulphate gel electrophoresis and by immunodiffusion. pantoate and dimethylmal ... | 1978 | 99175 |
| aspartate carbamyltransferase (pseudomonas fluorescens). | | 1978 | 99636 |
| [amino acid makeup characteristics of bacteria utilizing nonnatural compounds]. | a peculiarity of the amino acid pool has been discovered for the first time in bacteria utilizing such synthetic compounds as gamma butyrolactam, epsilon-caprolactam and zeta-aminoenanthic acid. the main components of the amino acid pool are omega-aminoacids (including synthetic ones) as well as glutamic acid. the total amino acid content increases upon utilization of the compounds being tested three times (pseudomonas fluorescens), five times (pseudomonas dacunchae) and seven times (pseudomonas ... | 1978 | 100669 |
| expression of escherichia coli k-12 arginine genes in pseudomonas fluorescens. | escherichia coli arge and argh gene products were detected in pseudomonas fluorescens argh122 carrying the e. coli f110 plasmid. | 1978 | 102635 |
| bacterial attack on phenolic ethers. purification and characterization of the components of the meta o-dealkylase of pseudomonas fluorescens tp. | the meta o-dealkylase of pseudomonas fluorescens tp has been resolved into two protein components, neither of which is a cytochrome. the substrate binding terminal oxidase has been purified and shown to be a non-haem iron protein of approximate molecular weight 118,000, consisting of two seemingly identical subunits, each of molecular weight 55,000. binding of substrate by the terminal oxidase has been established by difference spectroscopy. the amino acid composition of the protein has also bee ... | 1977 | 104127 |
| [isolation and characterization of lipase from pseudomonas fluorescens 533-5b]. | the enzyme was isolated from the culture fluid of pseudomonas fluorescens 533-5b and purified by precipitation with (nh4)so4 and acetone and by gel filtration through sephadex g-200. the enzyme was found homogeneous during polyacrylamide gel disc electrophoresis. the effects of metal ions, inhibitors, bile salts, temperature, ph and the substrate specificity of the enzyme were studied. it was shown that the enzyme from ps. fluorescens 533-5b has a broad specificity. it can use as substrates many ... | 1979 | 105765 |
| thermal inactivation of a heat-resistant lipase produced by the psychotrophic bacterium pseudomonas fluorescens. | lipase from pseudomonas fluorescens was studied for thermostability at temperatures ranging from 100 c to 160 c. the heat treatments were in two media, and heating times necessary to inactivate 90% of the enzyme at constant temperature were extremely long even at high temperatures, e.g. 3.6 min at 140 c in nutrient broth and 2.0 min at 170 c in skim milk. the increments of temperature to reduce these heating times 90% were 37.0 c in nutrient broth and 38.9 c in skim milk. the lipase was inactiva ... | 1979 | 109478 |
| microorganisms in the rhizosphere of wheat colonized by the fungus gaeumannomyces graminis var. tritici. | the population of microorganisms in wheat rhizosphere changed in the presence of the fungus gaeumannomyces graminis var. tritici causing the take-all of wheat. in the majority of cases when the soil was artificially contaminated by the fungus, both the number of bacteria in the rhizosphere and the bacteria/fungi ratio temporarily increased. at the beginning bacteria growing in the presence of nh4+ predominated, later bacteria utilizing organic n-substances prevailed. pseudomonas fluorescens and ... | 1979 | 112016 |
| effect of temperature on the uptake of glucose, gluconate, and 2-ketogluconate by pseudomonas fluorescens. | | 1978 | 113064 |
| malonyl-coa decarboxylase from mycobacterium tuberculosis and pseudomonas fluorescens. | | 1979 | 114118 |
| a study of p-hydroxybenzoate hydroxylase from pseudomonas fluorescens. improved purification, relative molecular mass, and amino acid composition. | the purification procedure for p-hydroxybenzoate hydroxylase has been modified by replacement of the deae-cellulose (de-32) column in the original procedure by a sephadex--cibacron-blue affinity column. in this way the yield of enzyme could be improved from 16% to about 40--50%. preparative gel chromatography indicated that the enzyme does not exist as a monomeric species as earlier believed but mainly as a dimer. sodium dodecyl sulfate gel electrophoresis of purified enzyme revealed a minimum r ... | 1979 | 116851 |
| [synthesis of l-aspartic acid by escherichia coli and pseudomonas fluorescens as related to the cultivation conditions]. | the capacity of the cultures escherichia coli str. 85, 113, bc, c and k-12 and pseudomonas fluorescens str. 1 to synthesize l-aspartic acid from fumarate and ammonium ions was studied. e. coli str. 85 was shown to synthesize the largest amounts of aspartic acid. the cultivation conditions which helped to increase the activity several times were selected. the product of fumarate amination by ammonium ions was identified and found to be l-isomer of aspartic acid with an angle of rotation of [alpha ... | 1979 | 117446 |
| effect of carbon dioxide on growth of pseudomonas fluorescens. | in minimal medium at 30 degrees c, growth of pseudomonas fluorescens was stimulated when the pressure (p) of co2 in solution was 100 mm of hg, but at higher concentrations the growth rate declined linearly with increasing pco2. all concentrations of co2 were inhibitory for growth in complex medium, and at 30 degrees c the maximum degree of inhibition was attained when pco2 was 250 mm of hg. the degree of inhibition at a constant pco2 in solution increased with decreasing temperature. the degree ... | 1979 | 117751 |
| cadmium and zinc sensitivity and tolerance in bacillus subtilis subsp. niger and in a pseudomonas sp. | the action of cd2+ and zn2+ on bacillus subtilis subsp. niger atcc 9372, and on a pseudomonas sp. (possibly pseudomonas fluorescens), isolated from cadmium-polluted soil has been determined and compared with results obtained previously with klebsiella aerogenes. in liquid medium the lag and the mean generation time of bacillus subtilis subsp, niger increased with increasing cd2+ or zn2+ concentrations whereas only the total biomass of the pseudomonas sp. was affected. nevertheless, the responses ... | 1979 | 119131 |
| sorption and desorption of atrazine by three bacterial species isolated from aquatic systems. | the isolates acinetobacter spec., cytophaga spec. and pseudomonas fluorescens represent different morphological and physiological types of bacteria. they accumulate atrazine (2-chloro-4-(ethylamino)-6-(isopropylamino)-5-triazine) to different levels. accumulation expressed by the ratio of atrazine sorbed per volume of bacteria to atrazine per the same volume of water amounted to 11 for acinetobacter spec., 8.6 for cytophaga spec. and 6.2 for pseudomonas fluorescens. accumulation ratios were prop ... | 1979 | 119497 |
| [solubilization effect of phosphorus by aspergillus niger and pseudomonas fluorescens]. | | 1977 | 121319 |
| [factors influencing the growth and survival in vittel mineral water of various bacteria of intestinal origin and of a strain of pseudomonas fluorescens]. | | 1979 | 121453 |
| comparative specificities of trehalases from various species. | 1. using derivatives or non-symmetrical analogs of alpha,alpha-trehalose, we studied the catalytic specificities of trehalases from various species: pseudomonas fluorescens, melolontha vulgaris, porcine and human kidneys. 2. alpha,beta-trehalose, beta,beta-trehalose, 6,6'dideoxy alpha,alpha-trehalose, alpha-d-xylopyranosyl alpha-d-xylopyranoside were shown to be neither substrates nor inhibitors. 3. 6'deoxy alpha,alpha-trehalose, alpha-d-glucopyranosyl alpha-d-xylopyranoside, alpha-d-allopyranos ... | 1978 | 122569 |
| [effect of a beryllium complex on growth of pseudomonas fluorescens (types r and s). i. influence on the lag phase]. | the toxicity of beryllium for pseudomonas fluorescens is studied with the aid of an anionic complex of the element which is stable in peptone medium at ph 6 during the period of investigation. the toxic effect is characterized by an increase in the lag phase which is proportional to the square of the beryllium concentration. further, a process of progressive adaptation is observed when the concentration of the beryllium complex is gradually increased. | 1975 | 126778 |
| [effect of a beryllium complex on growth of pseudomonas fluorescens (types r and s). ii. competition with magnesium]. | a study of the inhibitory action of beryllium on the growth of pseudomonas fluorescens reveals that the observed effect can be partly explained by competition between beryllium and magnesium in various processes which are indispensable to cellular metabolism. in addition, an "adaptation" phenomenon is observed which appears to be based on the selection of cells which are more highly resistant towards the inhibitor. | 1975 | 126779 |
| [mechanism of glucose oxidation by a pseudomonas fluorescens strain (type r). iii. influence of endogenous non-protein factors]. | cells of p. fluorescens cultivated in iron-deficient media contain two types of constituents, one of which can activate and the other reduce the rate of oxidation of glucose and of the endogenous substrates. these factors, which are of non-protein nature, have been distinguished and characterized by their physiological activity on non-proliferating cells suspensions of these bacteria. | 1975 | 131630 |
| [assay of gluconic acid by the gluconodehydrogenase of pseudomonas fluorescens]. | a method is outlined for the preparation of a soluble form of glucono-dehydrogenase from p. fluorescens. the use of this enzyme enables a simple, specific and accurate determination of gluconic acid in complex biological media. | 1976 | 140000 |
| fructose metabolism in four pseudomonas species. | 1. atp-dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of pseudomonas extorquens strain 16, pseudomonas 3a2, pseudomonas acidovorans and pseudomonas fluorescens. instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. such an activity could not be detected in cell-free extracts of succinate-grown cells. 2. high levels of 1-phosphofructokinase were ... | 1977 | 143919 |
| catalase induction in non-proliferating suspensions of pseudomonas fluorescens (type r.). i. influence of the buffer]. | the catalasic activity of a non-proliferating suspension of pseudomonas fluorescens incubated in a buffer shows after six hours, an increase of 100% in presence of phosphates and 30% in presence of tris. this increase, which consists in a neosynthesis of the enzyme, is in relation with the lysis of a part of the bacterial suspension. | 1977 | 146556 |
| [effect of dichlorophenolindophenol on glucose dehydrogenase activity of pseudomonas fluorescens (type r)]. | the glucose dehydrogenase activity of pseudomonas fluorescens cells grown in iron-depleted synthetic media is strongly decreased (about 80%) by dichlorophenolindophenol (dip) 2 x 10(-3) m. in those cells, dip seems not to play the part of an ultimate electron acceptor. | 1978 | 154953 |
| [activation, by various aldoses, of dichlorophenol-indophenol reduction by endogenous constituents of a preparation of glucose dehydrogenase from pseudomonas fluorescens]. | dichlorophenol-indophenol is reduced neither by d-glucose nor by the endogenous components of a particulate purified glucose-dehydrogenase from pseudomonas fluorescens, when these two classes of compounds acts individually. in contrast, the dye is quickly reduced by the endogenous components when the reaction occurs in the presence of glucose, without a direct participation of glucose in the reduction. in this effect d-glucose can be replaced by d-mannose, d-galactose or d-xylose, but not by d-f ... | 1979 | 160821 |
| [distribution of bdellovibrio bacteriovorus parasitizing pseudomonas fluorescens and serratia marcescens in river water]. | | 1979 | 160878 |
| inactivation of viruses and bacteria by ozone, with and without sonication. | selected organisms with public health significance were placed in a reaction chamber for treatment by ozonation, by ozonation and sonication, by sonication, or by sonication during oxygenation. vesicular stomatitis virus, encephalomyocarditis virus, gdvii virus, staphylococcus aureus, pseudomonas fluorescens, salmonella typhimurium, enteropathogenic escherichia coli, vibrio cholerae, and shigella flexneri were inactivated by treatment with ozone. when microorganisms were suspended in phosphate-b ... | 1975 | 163616 |
| the specificity of oxidase and kinase preparations from pseudomonas fluorescens towards deoxyfluoromonosaccharides. | with partially purified enzyme preparations from cell-free extracts of pseudomonas fluorescens, 3-deoxy-3-fluoro-d-glucose and 3-deoxy-3-fluoro-d-gluconic acid are substrates for glucose oxidase (ec 1.1.3.4.) and gluconate dehydrogenase (ec 1.1.99.3), with k-m values 18.2 mm and 11.8 mm, respectively. the same enzymes that oxidize glucose and gluconic acid probably oxidize 3-deoxy-3-fluoro-d-glucose and 3-deoxy-3-fluoro-d-gluconic acid. the latter fluorinated carbohydrates and the presumed forma ... | 1975 | 164267 |
| kynureninase-type enzymes of penicillum roqueforti, aspergillus niger, rhizopus stolonifer, and pseudomonas fluorescens: further evidence for distinct kynureninase and hydroxykynureninase activities. | the kynureninase-type enzymes of three fungi and one bacterium were isolated and examined kinetically for their ability to catalyze the hydrolysis of l-kynurenine and l-3-hydroxykynurenine. the phycomycete rhizopus stolonifer was found to contain a single, constitutive enzyme with km for l-3-hydroxykynurenine and l-kynurenine of 6.67 times 10-minus 6 and 2.5 times 10-minus 4 m, respectively. the ascomycetes aspergillus niger and penicillium roqueforti each contain an enzyme, induced by l-tryptop ... | 1975 | 164432 |
| production of hydrogen cyanide by pseudomonas fluorescens. | two pseudomonas fluorescens isolates were found to produce hydrogen cyanide when cultured on either trypticase soy agar supplemented with 0.5% yeast extract or on irradiation-sterilized chicken. | 1975 | 164822 |
| stereochemistry of the hydrogen transfer to nad catalyzed by d-galactose dehydrogenase from pseudomonas fluorescens. | the stereochemistry of the hydrogen transfer to nad catalyzed by d-galactose dehydrogenase (e.c. 1.1.1.48) from p. fluorescens was investigated. the label at c-1 of d-[1--3h] galactose was enzymatically transferred to nad and the resulting [4--3h]nadh was isolated and its stereochemistry at c-4 investigated. it was found that the label was exclusively located at the 4(s) position in nadh which calls for classification as a b-enzyme. this result was confirmed by an alternate approach in which [4- ... | 1975 | 167851 |
| effect of temperature on the activity and synthesis of glucose-catabolizing enzymes in pseudomonas fluorescens. | the activity of the enzymes of the oxidative non-phosphorylated pathway, glucose and gluconate dehydrogenases, were not significantly affected by changes in the assay temperature. both enzymes demonstrated only a threefold difference in activity when compared at assay temperatures of 30 degrees c and 5 degrees c. in contrast, the enzymes involved in the direct phosphorylation and catabolism of glucose or its oxidation products, gluconate and 2-ketogluconate, exhibited a more pronounced response ... | 1975 | 172202 |
| antibacterial activity of 15-azasteroids alone and in combination with antibiotics. | a new class of 15-azasteroid analogues has been synthesized and tested for antimicrobial activity. the compounds 1, 10, 11, 11a-tetrahydro-7-methoxy-11a-methyl-2h-naphth (1,2-g) indol (methoxyimine) and 1,10,11,11a-tetrahydro-11a-methyl-2h-naphth (1,2-g) indol-7-ol (hydroxyimine) inhibit the growth of bacillus subtillis and escherichia coli at concentrations as low as 10-5 m. addition of either compound to the growth medium casued a rapid inhibition in the transport of radioactive glucose, uraci ... | 1976 | 179169 |
| on the prosthetic groups of l-tryptophan 2,3-dioxygenase from pseudomonas: evidence for noninvolvement of copper in the reaction. | the amounts of copper present in highly purified preparations of l-tryptophan 2,3-dioxygenase from pseudomonas fluorescens have been shown to be negligible by six different methods of copper determination. it has also been demonstrated that, during the purification, the heme content of enzyme preparations increased in parallel with the specific enzyme activity, whereas that of copper decreased. these results, together with the finding that the inhibitory effects of copper chelators on the enzyme ... | 1976 | 183474 |
| effect of iron-beryllium antagonism on the growth of pseudomonas fluorescens type s. | studies of the growth of pseudomonas fluorescens type s in acidified peptone nutrient broth supplemented with potassium dioxalatoberyllate show that the inhibitory action of beryllium on the lag phase can be strongly counteracted by an increase in the iron content of the medium. in terms of relative concentrations, fe3+ is up to 250 times more effective than mg2+ as an antagonist of beryllium under the conditions employed. conversely, evidence of the effect of beryllium on iron-dependent constit ... | 1977 | 190091 |
| [the endogenous metabolism of pseudomonas fluorescens in relation to the oxidation of ethanol, serine, and pyruvate]. | studies of the oxidation of ethanol, serine and pyruvate by non proliferating suspensions of p. fluorescens show that the rate of oxidation of these substrates is appreciably increased by the addition of nadh. the observed effects are interpreted on the basis of apparent oxidation quotients of nadh which exceed the theoretical values. | 1976 | 192421 |
| kynureninase-type enzymes and the evolution of the aerobic tryptophan-to-nicotinamide adenine dinucleotide pathway. | kynureninase-type (l-kynurenine hydrolase, ec 3.7.1.3) activity has been found to be present in the livers of fish, amphibia, reptiles, and birds. in addition to past information concerning this enzyme activity in mammalian liver, it is now clear that all the major classes of vertebrates carry a highly specialized kynureninase-type enzyme, which we have termed a hydroxykynureninase. to compare the reactivities of these enzymes with l-kynurenine and l-3-hydroxykynurenine, ratios of tau values (km ... | 1977 | 195620 |
| nitrous oxide as end product of denitrification by strains of fluorescent pseudomonads. | growing cultures of several strains of pseudomonas fluorescens and pseudomonas chlororaphis produced n2o as the only detectable gaseous product of denitrification, and other strains produced n2 as the gaseous end product of denitrification. all of the nitrogen in no3- or no2- added to cell suspensions of the n2o-producing strains p. fluorescens pj 185 and p. chlororaphis b-560 was recovered as n2o. all of the nitrogen in no3- or no2- added to cell suspensions of the n2-producing strain p. fluore ... | 1977 | 195699 |
| induction of polymyxin resistance in pseudomonas fluorescens by phosphate limitation. | shift of pseudomonas fluorescens ncmb 129 from a a phosphate rich into a phosphate limited medium results in a reduction of the membrane phospholipids phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. concomitantly a positively charged ornithine amide lipid is synthesized. the gradual increase of this lipid is paralleled by an increasing resistance to polymyxin b. the binding capacities of intact cells, and isolated inner and outer membranes for the antibiotic are reduced in the re ... | 1977 | 199125 |
| [effect of nadh and several krebs cycle substrates on the endogenous metabolism of pseudomonas fluorescens (type s)]. | simultaneous addition of succinate (or fumarate) and nadh to a non-proliferating suspension of p. fluorescens results in an enhancement of the oxydation of the endogenous substrates. this conclusion is in accord with the determination of the respiratory and the oxidation quotients of the analysed exogenous substrates. | 1977 | 201351 |
| effect of temperature on diauxic growth with glucose and organic acids in pseudomonas fluorescens. | growth of pseudomonas fluorescens in batch culture with glucose and organic acids resulted in typical diauxic responses at 30 degrees c but no detectable diauxic lag at 5 degrees c. at 30 degrees c, organic acids were preferentially utilized during the first growth phase. glucose utilization was delayed until onset of the second growth phase. systems involved in direct uptake and catabolism of glucose responded in a manner compatible with repression by malate and/or its metabolites and induction ... | 1978 | 211975 |
| adsorption of vibrio parahaemolyticus onto chitin and copepods. | vibrio parahaemolyticus was observed to adsorb onto chitin particles and copepods. the efficiency of adsorption was found to be dependent on ph and on the concentration of nac1 and other ions found in seawater. highest efficiency was observed in water samples collected from chesapeake bay and lowest in water from the open sea. v. parahaemolyticus was found to adborb onto chitin with the highest efficiency of the several bacterial strains tested. escherichia coli and pseudomonas fluorescens d ... | 1975 | 234715 |
| stereospecificity of hydrogen transfer catalyzed by d-galactose dehydrogenase from pseudomonas saccharophila and pseudomonas fluorescens. | | 1975 | 237553 |
| allantoin racemase: a new enzyme from pseudomonas species. | 1. allantoin racemase is a novel enzyme which catalyzes the conversion of s(+)-and r(minus)-allantoin into the racemate. 2. the enzyme is present in pseudomonas testosteroni, pseudomonas putida and five biotypes of pseudomonas fluorescens, but absent in a number of other pseudomonas species. 3. the enzyme of ps. testosteroni was purified 133-fold and exposes optimal activity at ph 8.0-8.2 and 50 degrees c. the enzyme is stable on heating for 15 min at 70 degrees c. 4. the enzyme appeared to be s ... | 1975 | 237557 |
| [change in the indices of direct count of bacteria in cultures and pond water at different ph]. | experiments were carried out with the pure cultures of pseudomonas fluorescens and bacillus subtilis, and with pond water. the adjustment of ph of the culture or water to 8.8--9.0 facilitates the direct count of the microorganisms, and makes the results more accurate. | 1975 | 240104 |
| maltose metabolism of pseudomonas fluorescens. | pseudomonas fluorescens w uses maltose exclusively by hydrolyzing it to glucose via an inducible alpha-glucosidase (alpha-d-glucoside glucohydrolase, ec 3.2.1.20). no evidence for phosphorolytic cleavage or oxidation to maltobionic acid was found in this organism. the alpha-glucosidase was totally intracellular and was most active at ph of 7.0. induction occurred when cells were incubated with maltotriose or maltose. induction was rapid and easily detectable within the first 5 min after the addi ... | 1975 | 240805 |
| role of the 30s ribosomal subunit, initiation factors, and specific ion concentration in barotolerant protein synthesis in pseudomonas bathycetes. | washed (1 m nh4cl) ribosomes from pseudomonas bathycetes, pseudomonas fluorescens, and escherichia coli were tested for their ability to synthesize protein or polypeptide at high pressure when used as such, when recombined with homologous initiation factors, and when recombined with heterologous initiation factors. the responses of natural messenger ribonucleic acid (ms-2)-directed systems to pressure were independent of the source of initiation factors and paralleled those of the washed ribosom ... | 1977 | 323224 |
| nonfermentative bacilli: evaluation of three systems for identification. | three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ... | 1979 | 389945 |
| pseudomonic acid. part 1. the structure of pseudomonic acid a, a novel antibiotic produced by pseudomonas fluorescens. | | 1977 | 402371 |
| tyrosine emission in the tryptophanless azurin from pseudomonas fluorescens. | a strain of pseudomonas fluorescens contains an azurin with no tryptophan and two tyrosines. this protein is interesting because it allows one to study both the structure of azurin and the emission of tyrosines in proteins. comprehensive measurements were carried out including spectrophotometric and fluorimetric titration, fluorescence quantum yield, fluorescence polarization, and i- quenching. in the copper-containing protein, almost independent of the copper ion oxidation, the fluorescence qua ... | 1977 | 402931 |
| altered regulation of macromolecular synthesis in methionine-inhibited cultures of pseudomonas fluorescens uk1. | effects of high methionine concentrations on growth of pseudomonas fluorescens uk1 are reported. the following phenomena were observed: (i) immediate inhibition of growth for a period corresponding to approximately half a generation. steady-state conditions of growth were no more attained. (ii) in spite of stringency of the macromolecular synthesis in this organism, simultaneously with the growth inhibition, the rate of labelled leucine incorporation into trichloroacetic acid (tca)-insoluble mat ... | 1977 | 403021 |
| synthesis and antimicrobial activity of azasteroid-type compounds and related systems. effect of hydrophilic and lipophilic groups on activity. | pyrazole-, pyrazolone- and isoxazole-containing systems were prepared from 3,4-dihydro-6-(hexyloxy)-1(2h)-naphthalenone, 3,4-dihydro-6-(hexadecyloxy)-1(2h)-naphthalenone,3,4-dihydro-6(2-dimethylaminoethyloxy)-1-(2h)-naphthalenone, 3,4-dihydro-7-hexyloxy-1(2h)-phenanthrone, and 3,4-dihydro-7-(2-dimethylaminoethyloxy)-1(2h)-phenanthrone. a number of compounds derived from 7, 8-dihydro-5(6h)-quinolinone were also synthesized and characterized. both hydrophilic and lipophilic groups were incorporate ... | 1977 | 404424 |
| catabolism of l-lysine by pseudomonas aeruginosa. | pseudomonas aeruginosa paci grows poorly on l-lysine as sole source of carbon but mutant derivatives which grow rapidly were readily isolated. studies with one such mutant, p. aeruginosa pac586, supported the existence of a route for l-lysine catabolism which differes from those reported previously in other species of pseudomonas. the postulated route, the cadaverine or decarboxylase pathway, is initiated by the decarboxylation of l-lysine and involves the following steps: l-lysine leads to cadv ... | 1977 | 405455 |
| uptake of beryllium-7 in cultures of pseudomonas fluorescens grown in natural and synthetic media. | | 1977 | 405806 |
| antagonistic interactions of phylloplane bacteria with drechslera dictyoides (drechslera) shoemaker. | strains of listeria denitrificans (e2), pseudomonas fluorescens (c37 and c92), and xanthomonas campestris (d119), isolated from the phylloplane of lolium perenne (s24), were antagonistic to drechslera dictyoides (drechslera) shoemaker. from in vitro and in vivo experiments it was deduced that their mode of activity included an initial inhibition of spore germination, a retardation in the rate of germ-tube elongation, and ultimately lysis of the hyphae. the effects were expressed on the plant in ... | 1977 | 406025 |
| synthesis and biological activity of some derivatives of thiochroman-4-one and tetrahydrothiapyran-4-one. | a small series of pyrazoles and isoxazoles derived from thiochroman-4-one has been synthesized and characterized. the compounds were examined for their in vitro inhibitory activity against bacillus subtilis and pseudomonas fluorescens. among the tested compounds the pyrazole derivative from thiochroman-4-one was found to be the most effective inhibitor of growth of b. subtilis. extensive h nmr analysis was recorded for all compounds. | 1977 | 406397 |
| diluent sensitivity in thermally stressed cells of pseudomonas fluorescens. | thermally injured cells of pseudomonas fluorescens were unable to produce colonies on trypticase soy agar (tsa) after dilution with 0.1% peptone. nutritional exigency could not be used as the criterion for this injury, since varying the composition of the plating medium had little effect on the number of colonies that developed. the injured cells had no requirement for compounds known to leak out during the heat treatment in order to recover. the cells did not exhibit injury if dilution preceded ... | 1977 | 406839 |
| the interaction of deoxyfluoro substrate-analogues with d-galactose dehydrogenase from pseudomonas fluorescens. | | 1977 | 409498 |
| [effect of formaldehyde on a pseudomonas fluorescens strain]. | the strain of pseudomonas fluorescens was isolated from active ooze and was capable of growth on a medium containing 100 mg/litre of formaldehyde. as a result of stepwise selection, a variant of ps. fluorescens 27 oxidizing 250 mg/litre of formaldehyde was obtained. it differed from the parent strain in cultural, morphological, and biochemical properties. | 1977 | 409910 |
| [transport and excretion of a fluorescent pigment by cells of a pseudomonas fluorescens culture]. | the transport and exretion of a yellow-green pigment were studied with the culture of pseudomonas fluorescens k-1. these processes were found to be accomplished by means of membranous formations having a lamellar structure. | 1977 | 409911 |
| chemotaxis by bdellovibrio bacteriovorus toward prey. | a chemotaxis assay system that uses a modified boyden chamber was characterized and used for measurements of chemotaxis by bdellovibrio bacteriovorus strain uki2 toward several bacterial species. bacteria tested included both susceptible and nonsusceptible cells (escherichia coli, pseudomonas fluorescens, bacillus megaterium, and b. bacteriovorus strains uki2 and d). none was attractive to bdellovibrios when present at densities below 10(7) cells per ml. chemotaxis toward e. coli was studied mos ... | 1977 | 410796 |
| [physico-chemical properties of deamidase ag from pseudomonas fluorescens ag possessing antitumor activity]. | homogenous deamidase ag from pseudomonas fluorescens ag was found to be a glycoprotein with molecular weight of about 13,000 daltons. the molecule consists apparently of four similar or identic subunits with molecular weight of about 30,000 daltons. the amino acid composition, n-terminal amino acids, the amount of chymotryptic peptides, containing 14c-carboxymethyl cysteine were studied. the enzyme exhibited distinct antitumoral effect on cells of burkitt's lymphoma, sensitive to asparaginase, b ... | 1977 | 411253 |
| some properties of the extracellular protease produced by the psychotrophic bacterium pseudomonas fluorescens strain ar-11. | the major extracellular protease from pseudomonas fluorescens strain ar-11 has been partially purified by a factor of 300 by a combination of deae-cellulose ion-exchange chromatography and gel filtration. the enzyme had a molecular weight of 38 400 and exhibited optimum activity with isoelectrically precipitated casein substrate at ph 6.5 with km - 0.13 mm. the protease was strongly inhibited by a number of heavy metal ions at the 10 mm level and also inhibited by thiol agents, while 10 mm edta ... | 1977 | 411520 |
| physicochemical properties of a lipase from pseudomonas fluorescens. | the molecular weight of traicylglycerol lipase (ec 3.1.1.3) from pseudomonas fluorescens is estimated to be approx. 33 000 by sodium dodecyl sulfate electrophoresis and sephadex g-75 gel filtration. the lipase appears to be a single-chain protein and contains neither sugar nor lipid. the enzyme has a sedimentation coefficient (s20,w) of 3.06, an intrinsic viscosity of 3.0 g/ml and a partial specific volume of 0.730 g/ml, with an isoelectric point of ph 4.46. amino acid analysis showed that the e ... | 1977 | 411521 |
| [substrate specificity, inhibitors and kinetics of deamidase ag (asparaginase-glutaminase) from pseudomonas fluorescens ag]. | deamidase ag (asparaginase-glutaminase) from pseudomonas fluorescens ag was shown to hydrolyze 1-glutamine and 1-asparagine highly effectively. besides, the enzyme exhibited the rather high rate of deamidation of d-asparagine and d-glutamine (70% and 100%, respectively), nalpha-butyl asparagine (63%) and among peptides -- of glycyl-l-asparagine (40%). l-glutamic acid gamma-methyl ester was hydrolyzed only slightly (5%). effect of several substrate analogues on the deamidase ag activity was studi ... | 1977 | 413263 |
| [effect of pseudomonas fluorescens on protein decomposition in milk (author's transl)]. | | 1977 | 413610 |
| nosocomial meningitis and bacteremia due to contaminated amphotericin b. | nosocomial gram-negative bacillary meningitis and bacteremia occurred in a patient who was receiving intrathecal and intravenous amphotericin b. an epidemiologic investigation found the amphotericin b to be contaminated with enterobacter agglomerans, pseudomonas fluorescens, and p aeruginosa. these contaminants were traced to a lot ot sodium phosphate buffer that was added to all intrathecal and intravenous amphotericin b preparations. the phosphate buffer underwent prolonged storage at room tem ... | 1978 | 413939 |
| [metabolic products of hydrocarbon-oxidizing strains of mycococcus lactis and pseudomonas fluorescens and their influence on culture growth]. | the effect of concentrations of organic acids in the cultural broth on growth was studied with the strains of mycococcus lactis and pseudomonas fluorescens oxidizing hydrocarbons. the ratio between the acid and neutral fractions in the cultural broth of mycococcus lactis was also investigated as well as their action on the bacterial growth. mycococcus lactis growing on paraffin was found to produce acid and neutral products at a ratio of 1:2. the acid and neutral products of metabolism produced ... | 1977 | 414057 |
| utilization of benzylpenicillin as carbon, nitrogen and energy source by a pseudomonas fluorescens strain. | a bacterium which utilizes benzylpenicillin as carbon, nitrogen and energy source was isolated from a lake sediment. the organism was identified as a strain of pseudomonas fluorescens with a gc content of 59.71 mol%. after growth of the organism on a mineral salts medium containing benzylpenicillin, the derivatives benzylpenicilloic acid, benzylpenilloic acid and benzylpenicillenic acid were found in culture media. there was no indication that the phenylacetate side chain of benzylpenicillin is ... | 1977 | 414683 |
| [biological properties of an asparaginase-glutaminase preparation from pseudomonas fluorescens in cell cultures]. | specific l-asparaginase activity and non-specific cytotoxicity of asparaginase-glutaminase preparation from pseudomonas fluorescens were studied. two cell lines, i.e. the asparaginase-dependent (berkitt lymphoma cells) and the asparaginase-independent (the ovary cancer cells) were used as the test-system. incorporation of 3h-timidine into dna was used as the criterion of the drug effect on the cells. krasnitin was used as the reference preparation. the preparation of asparaginase-glutaminase was ... | 1978 | 415658 |
| the effect of water activity on the growth and respiration of pseudomonas fluorescens. | | 1978 | 416013 |
| [effect of phosphorylated constituents on the growth of pseudomonas fluorescens in media containing potassium oxalatoberyllate]. | differences in the extent of beryllium inhibition on the growth of pseudomonas fluorescens cultivated in various media are related to the concentration and nature of phosphorus-containing constituents of the substrate. quantitative studies are carried out for interaction processes leading to reduction in beryllium toxicity. these include sequestration of beryllium in soluble form by beta-glycerophosphate and formation of sparingly soluble derivatives with inorganic orthophosphate ions. | 1977 | 417830 |
| thermal stress of pseudomonas fluorescens in complex media. | pseudomonas fluorescens (p7) cells were stressed by incubation at 43 degrees c for 2 h. the stress induced a 9-h lag in replication after the return of the temperature of the culture to 25 degrees c. stressed cells demonstrated a sensitivity to diluents and plating media during the recovery period. data from utilization of selective inhibitors suggested that ribonucleic acid and protein, but not deoxyribonucleic acid, syntheses were required for recovery by the cells. the cells lost uracil- and ... | 1979 | 453826 |
| [microbiologic studies of a spodumene deposit]. | a wide spectrum of heterotrophic and autotrophic microorganisms was detected in the zones of decomposition of spodumene and bed rocks, pegmatites and shales, in the spodumene deposit. the following aerobic microorganisms which did not from spores predominated in the deposit: arthrobacter globiformis, a. pascens, a. simplex, nocardia globerula, pseudomonas fluorescens, ps. putida, ps. testosteronii. the following specific bacterial groups were found: thionic, sulfate reducing, and nitrifying bact ... | 1979 | 470634 |
| a simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase. | a simplified enzymic procedure to determine accurately serum triglycerides is described. serum triglycerides are hydrolyzed completely to free fatty acids and glycerol by lipoprotein lipase from pseudomonas fluorescens. the released glycerol is oxidized with glycerol dehydrogenase from erwinia aroideae in the presence of nad+, were the reduction of the enzyme-linked nad+ is coupled to the reduction of nitro blue tetrazolium as a chromogenic indicator with phenazine methosulfate serving as an int ... | 1977 | 589792 |