| polar lipids of pseudomonas vesicularis. presence of a heptosyldiacylglycerol. | the individual polar lipids produced by psuedomonas vesicularis nctc 10 900 during surface culture have been isolated. the major lipids are phosphatidylglycerol, a phosphatidyl-alpha-d-glucopyranosyldiacylglycerol, 1,2-di-o-acyl-3-o-alpha-d-glucopyranosylglycerol. 1,2-di-o-acyl-3-o-alpha-d-glucopyranuronosylglycerol, and 1,2-di-o-acyl-3-o[beta-d-glucopyranosyl-(1 yields 4)-alpha-d-glucopyranuronosyl]glycerol. these are also the major polar lipids of pseudomonas diminuta. additional lipids presen ... | 1979 | 508784 |
| degradation of bacterial cyclopropane acids with boron trihalide reagents. | the cellular fatty acid compositions of pseudomonas diminuta uc 501 and streptococcus mutans omz-61 were compared in samples processed by a saponification-methylation procedure (method s) and in samples processed by a transesterification procedure (method t). all samples were heated in an inert atmosphere of nitrogen. the major acids found in samples of p. diminuta treated by method s included 16:0 (palmitic), 18:1 (octadecenoic) and 19 cyc (19 carbon cyclopropane acid). those found in samples o ... | 1977 | 609335 |
| [application of the method of capillary microscopy to determing the total population and biomass of microorganisms in seas and oceans]. | the total number and biomass of microorganisms in samples of sea water were determined by direct counting in capillaries by perfiliev and gabe after preliminary concentration of water by filtering it through membrane filters. reliability of the figures obtained upon concentration of diluted bacterial suspensions (10(3) to 10(5) cells/ml) was checked with dilutions of the cell suspension of bacterium parvulum. the total number of the microorganisms was determined in 22 samples of sea water fixed ... | 1975 | 775259 |
| construction of a 7-aminocephalosporanic acid (7aca) biosynthetic operon and direct production of 7aca in acremonium chrysogenum. | we have used cdna encoding d-amino acid oxidase, and genomic dna encoding cephalosporin acylase from fusarium solani and pseudomonas diminuta, respectively, to construct a novel hybrid 7-aminocephalosporanic acid (7aca) biosynthetic operon under the control of regulatory elements from the alkaline protease gene of acremonium chrysogenum. transformants of a. chrysogenum bc2116, a high cephalosporin-producing strain, containing this operon, synthesized and secreted low levels of 7aca. although the ... | 1991 | 1369453 |
| bacterial retention properties of heat and moisture exchange filters. | we have examined the properties of six heat and moisture exchange filters (hmef) to ascertain their resistance to liquid flow and their ability to retain a challenge bacterium, pseudomonas diminuta, from aqueous and nebulized suspensions. only one hmef, the pall ultipor was able to withstand a significantly greater pressure of liquid than that found in clinical practice. however, when breached, the hmef were unable to prevent transmission of micro-organisms from aqueous suspension. only the dare ... | 1992 | 1467087 |
| effectiveness of cefetamet pivoxil in the treatment of pyelonephritis in children. | cefetamet pivoxil was investigated in an open, randomized comparative study involving a total of 37 children with acute pyelonephritis, whose ages ranged from 2 to 14 years. the patients received either 10 mg/kg (n = 18) or 20 mg/kg (n = 8) cefetamet pivoxil twice daily, or 30-50 mg/kg amoxycillin/clavulanic acid three times daily (n = 11) for a period of 7-10 days. escherichia coli was the main causative agent isolated in 28 (75.7%) of the patients; other pathogens included proteus mirabilis (t ... | 1992 | 1568523 |
| detoxification of organophosphate pesticides using a nylon based immobilized phosphotriesterase from pseudomonas diminuta. | a partially purified phophotriesterase was successfully immobilized onto nylon 6 and 66 membranes, nylon 11 powder, and nylon tubing. up to 9000 u of enzyme activity was immobilized onto 2000 cm2 of a nylon 6 membrane where 1 u is the amount of enzyme necessary to catalyze the hydrolysis of 1.0 mumol of paraoxon/min at 25 degrees c. the nylon 66 membrane-bound phosphotriesterase was characterized kinetically where the apparent km value for the immobilized enzyme was 0.35 mm. this is 5-6 times hi ... | 1991 | 1665681 |
| microbial metabolism of quinoline and related compounds. ix. degradation of 6-hydroxyquinoline and quinoline by pseudomonas diminuta 31/1 fa1 and bacillus circulans 31/2 a1. | two strains, using 6-hydroxyquinoline as sole source of energy, carbon and nitrogen, have been isolated. these bacteria, designated 31/1 fa1 and 31/2 a1, are also able to degrade quinoline. according to their physiological properties strain 31/1 fa1 has been identified as pseudomonas diminuta and strain 31/2 a1 as bacillus circulans. 6-hydroxy-2-oxo-1,2-dihydroquinoline was found as intermediate in the degradation of 6-hydroxyquinoline and quinoline. 2-oxo-1,2-dihydroquinoline was the first meta ... | 1991 | 1910576 |
| inactivation of organophosphorus nerve agents by the phosphotriesterase from pseudomonas diminuta. | the phosphotriesterase from pseudomonas diminuta was tested as a catalyst for the hydrolysis of phosphofluoridates. the purified enzyme has been shown to hydrolyze the phosphorus-fluorine bond of diisopropyl fluorophosphate, isopropyl methylphosphonofluoridate, and 1,2,2-trimethylpropylmethylphosphonofluoridate at ph 7.0, 25 degrees c, with turnover numbers of 41, 56, and 5 s-1, respectively. the enzymatic rate enhancement for the hydrolysis of sarin at ph 7.0 is 2.2 x 10(7). the turnover number ... | 1990 | 2154956 |
| expression of pseudomonas phosphotriesterase activity in the fall armyworm confers resistance to insecticides. | the gene encoding for the phosphotriesterase (opd) from pseudomonas diminuta has been subcloned into a baculovirus expression system. functional enzyme is produced when the recombinant baculovirus is used to infect either cultured spodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. the ld50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase. | 1990 | 2164956 |
| chemical and kinetic evidence for an essential histidine in the phosphotriesterase from pseudomonas diminuta. | the ph rate profile for the hydrolysis of diethyl-p-nitrophenyl phosphate catalyzed by the phosphotriesterase from pseudomonas diminuta shows a requirement for the deprotonation of an ionizable group for full catalytic activity. this functional group has an apparent pka of 6.1 +/- 0.1 at 25 degrees c, delta hion of 7.9 kcal/mol, and delta sion of -1.4 cal/k.mol. the enzyme is not inactivated in the presence of the chemical modification reagents dithiobis-(2-nitrobenzoate), methyl methane thiosul ... | 1990 | 2174875 |
| cytokine induction by lipopolysaccharide (lps) corresponds to lethal toxicity and is inhibited by nontoxic rhodobacter capsulatus lps. | many pathological effects of gram-negative bacteria are produced by their cell wall-derived lipopolysaccharides (lpss). differing pathogenicity of gram-negative lpss, however, may depend on their capacities to induce cytokines. thus, we studied the lethal toxicity of four nonenterobacterial lpss and compared it with their capacity to induce mononuclear cell (mnc)-derived interleukin-1 (il-1), interleukin-6 (il-6), and tumor necrosis factor (tnf). unstimulated mnc did not release these cytokines. ... | 1990 | 2228245 |
| epitope analysis of lipid a preparations from pseudomonas diminuta and pseudomonas vesicularis. | in vitro antigenic reactivity of lipid a from pseudomonas diminuta and pseudomonas vesicularis with homologous and heterologous lipid a antibodies including monoclonal antibodies was studied by inhibition test of enzyme-linked immunosorbent assay (elisa). the results suggest that both pseudomonas lipid as have very similar epitopes, including species-specific and cross-reactive epitopes as compared with enterobacterial lipid a. | 1989 | 2476362 |
| structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from pseudomonas diminuta. | the mechanism and substrate specificity of the phosphotriesterase from pseudomonas diminuta have been examined. the enzyme hydrolyzes a large number of phosphotriester substrates in addition to paraoxon (diethyl p-nitrophenyl phosphate) and its thiophosphate analogue, parathion. the two ethyl groups in paraoxon can be changed to propyl and butyl groups, but the maximal velocity and km values decrease substantially. the enzyme will not hydrolyze phosphomonoesters or -diesters. there is a linear c ... | 1989 | 2548585 |
| practical methods for the microbial validation of sterilizing-grade filters used in aseptic processing. | microbial challenge test methods to validate sterilizing-grade filters were developed to facilitate the use of parenteral drug products as liquid vehicles. scaled-down filtration systems were developed in response to processing parameters, e.g., flow rates, pressures, duration, and temperature, provided by customers. accordingly, 47 mm disc filter holders were chosen to minimize the volume of product necessary for each test. a peristaltic pump was used to produce positive pressure within the tes ... | 1989 | 2689620 |
| isolation of 9-hydroxy-delta-tetradecalactone from lipid a of pseudomonas diminuta and pseudomonas vesicularis. | a lipid component was isolated from the fatty acid fraction of acid hydrolysates of lipid a derived from pseudomonas diminuta jcm 2788 and pseudomonas vesicularis jcm 1477 lipopolysaccharides. by structural analysis of the lipid and its trimethylsilyl and acetyl derivatives by thin-layer chromatography, gas chromatography-mass spectrometry, mass spectrometry, infrared spectrometry and 13c-nmr, it was identified as 9-hydroxy-delta-tetradecalactone. | 1989 | 2777066 |
| physical comparison of parathion hydrolase plasmids from pseudomonas diminuta and flavobacterium sp. | restriction maps of two plasmids encoding parathion hydrolase have been determined. ppdl2 is a 39-kb plasmid harbored by flavobacterium sp. (atcc 27551), while pcms1 is a 70-kb plasmid found in pseudomonas diminuta (strain mg). both plasmids previously have been shown to share homologous parathion hydrolase genes (termed opd for organophosphate degradation) as judged by dna-dna hybridization and restriction mapping. in the present study, we conducted dna hybridization experiments using each of n ... | 1987 | 2829255 |
| different lipid a types in lipopolysaccharides of phototrophic and related non-phototrophic bacteria. | lipid a analyses confirm not only the present taxa of the purple nonsulfur bacteria (formerly rhodospirillaceae), but also phylogenetical relatedness of distinct phototrophic to distinct non-phototrophic bacteria, as was suggested by cataloguing 16s rrna. for example, lipid a with ester-bound 3-oh-10:0 and the rare amide-linked 3-oxo-14:0 is common to the phototrophic rhodobacter capsulatus and rhodobacter sphaeroides and also to paracoccus denitrificans and thiobacillus versutus. 'lipid adag' ( ... | 1988 | 3078741 |
| pseudomonas diminuta lps with a new endotoxic lipid a structure. | lipid a that contains mainly 2,3-diamino-2,3-dideoxy-d-glucose, phosphate and fatty acids in the molar ratio 2:1:5-6 was found in pseudomonas diminuta lipopolysaccharide. the lipid a was considered to have a diamino-sugar disaccharide structure that carries a nonglycosidic phosphomonoester group and amide-bound acyloxyacyl and 3-hydroxy fatty acyl groups. the lipopolysaccharide exhibited endotoxic activities including lethal toxicity, pyrogenicity, local shwartzman activity, body weight-decreasi ... | 1987 | 3827908 |
| structural heterogeneity regarding local shwartzman activity of lipid a. | the relation of chemical structure to local shwartzman activity of lipid a preparations purified by thin-layer chromatography from five bacterial strains was examined. two lipid a fractions from e. coli f515--ec-a2 and ec-a3--exhibited strong activity, similar to that of previous synthetic e. coli-type lipid a (compound 506 or la-15-pp). the ec-a3 fraction contained a component that appeared to be structurally identical to compound 506, and the main component of ec-a2 fraction was structurally s ... | 1988 | 3057331 |
| effect of osmotic stabilizers on 14 co 2 production by bacteria and blood. | evolution of (14)co(2) by whole blood as well as by diplococcus pneumoniae, haemophilus sp., pseudomonas aeruginosa, pseudomonas diminuta, and streptococcus pyogenes was examined by using the bactec system. the control medium was jli no. 6a culture vial containing 30 ml of enriched tryptic soy broth and 1.5 muci of (14)c-substrate. hypertonic media consisted of control medium with either 1 or 3% nacl, 10% sucrose, and 5%, 10%, or 15% dextran. the most deleterious treatment to bacteria was 3% nac ... | 1973 | 4144652 |
| the phosphoglucolipid from pseudomonas diminuta. | | 1971 | 4331785 |
| production of glutaric acid: a useful criterion for differentiating pseudomonas diminuta from pseudomonas vesiculare. | a gas-liquid chromatographic procedure was used to determine short-chain acids produced by pseudomonas diminuta and p. vesiculare after growth on trypticase soy agar. each of nine strains of p. diminuta produced glutaric acid, whereas none of the strains of p. vesiculare produced this acid. | 1974 | 4823425 |
| replacement of acidic phosphates by acidic glycolipids in pseudomonas diminuta. | | 1974 | 4833243 |
| biosynthesis of glucuronosyl diglyceride by particulate fractions of pseudomonas diminuta. | | 1972 | 5012164 |
| identification of a plasmid-borne parathion hydrolase gene from flavobacterium sp. by southern hybridization with opd from pseudomonas diminuta. | parathion hydrolases have been previously described for an american isolate of pseudomonas diminuta and a philippine isolate of flavobacterium sp. (atcc 27551). the gene which encodes the broad-spectrum organophosphate phosphotriesterase in p. diminuta has been shown by other investigators to be located on a 66-kilobase (kb) plasmid. the intact gene (opd, organophosphate-degrading gene) from this degradative plasmid was cloned into m13mp10 and found to express parathion hydrolase under control o ... | 1986 | 3015022 |
| taxonomy of the aerobic psuedomonads: pseudomonas diminuta and p. vesiculare. | | 1968 | 5721589 |
| isolation and characterization of n-long chain acyl aminoacylase from pseudomonas diminuta. | n-long chain acyl aminoacylase ii (enzyme ii) catalyzing the hydrolysis of n-long chain acyl amino acids was purified about 2,000-fold from the cell extracts of pseudomonas diminuta with 1.8% of activity yield. the purified enzyme was homogeneous on polyacrylamide gel electrophoresis and the molecular weight was 220,000. enzyme ii differed from n-long chain acyl aminoacylase i (enzyme i) in molecular weight, in substrate specificity, and in behavior toward temperature and ph. enzyme ii showed br ... | 1984 | 6501258 |
| peptidoglycan of rhodopseudomonas viridis: partial lack of n-acetyl substitution of glucosamine. | a lack of at least 70% of n-acetyl substitution of glucosamine in the glycan strands of the peptidoglycan from the gram-negative bacterium rhodopseudomonas viridis is reported. a disaccharide, very likely glcn beta(1 leads to 4) mur, was observed in hydrolysates of the isolated peptidoglycan. the disaccharide was not observed when peptidoglycan was n-acetylated before hydrolysis. the peptidoglycan of r. viridis was resistant to lysozyme but became sensitive after n-acetylation with acetic anhydr ... | 1982 | 7054141 |
| a new enzyme: long acyl aminoacylase from pseudomonas diminuta. | a new enzyme which hydrolyzes n-long chain acyl glutamic acid was found in cell-free extracts of pseudomonas diminuta. two active fractions (long acyl aminoacylase i and ii) were separated by deae-cellulose column chromatography. the long acyl aminoacylase i was purified about 650-fold, and the purified preparation was electrophoretically homogeneous. the molecular weight was estimated to be 300,000 by gel filtration. the enzyme was unique in its substrate specificity. it hydrolyzed only n-long ... | 1982 | 7096313 |
| mechanism and stereochemical course at phosphorus of the reaction catalyzed by a bacterial phosphotriesterase. | the reaction mechanism for the phosphotriesterase from pseudomonas diminuta has been examined. when paraoxon (diethyl 4-nitrophenyl phosphate) is hydrolyzed by this enzyme in oxygen-18-labeled water, the oxygen-18 label is found exclusively in the diethyl phosphate product. the absolute configurations for the (+) and (-) enantiomers of o-ethyl phenylphosphonothioic acid have been determined by x-ray diffraction structural determination of the individual crystalline 1-phenylethylamine salts. the ... | 1988 | 2835095 |
| comparative ribosomal protein sequence analyses of a phylogenetically defined genus, pseudomonas, and its relatives. | i analyzed various families of ribosomal proteins obtained from selected species belonging to the genus pseudomonas sensu stricto and allied organisms which were previously classified in the genus pseudomonas. partial amino acid sequencing of l30 preparations revealed that the strains which i examined could be divided into three clusters. the first cluster, which was assigned to the genus pseudomonas sensu stricto, included pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, and p ... | 1995 | 7727274 |
| cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase. | plasmid pcms1 was isolated from pseudomonas diminuta mg, a strain which constitutively hydrolyzes a broad spectrum of organophosphorus compounds. the native plasmid was restricted with psti, and individual dna fragments were subcloned into pbr322. a recombinant plasmid transformed into escherichia coli possessed weak hydrolytic activity, and southern blotting with the native plasmid dna verified that the dna sequence originated from pcms1. when the cloned 1.3-kilobase fragment was placed behind ... | 1988 | 2834339 |
| purification and properties of the phosphotriesterase from pseudomonas diminuta. | the phosphotriesterase produced from the opd cistron of pseudomonas diminuta was purified 1500-fold to homogeneity using a combination of gel filtration, ion exchange, hydrophobic, and dye matrix chromatographic steps. this is the first organophosphate triesterase or organophosphofluoridate hydrolyzing enzyme to be purified to homogeneity. the enzyme is a monomeric, spherical protein having a molecular weight of 39,000. a single zinc atom is bound to the enzyme and is required for catalytic acti ... | 1989 | 2555328 |
| molecular cloning of the isoquinoline 1-oxidoreductase genes from pseudomonas diminuta 7, structural analysis of iora and iorb, and sequence comparisons with other molybdenum-containing hydroxylases. | the iora and iorb genes from the isoquinoline-degrading bacterium pseudomonas diminuta 7, encoding the heterodimeric molybdo-iron-sulfur-protein isoquinoline 1-oxidoreductase, were cloned and sequenced. the deduced amino acid sequences iora and iorb showed homologies (i) to the small (gamma) and large (alpha) subunits of complex molybdenum-containing hydroxylases (alpha beta gamma/alpha 2 beta 2 gamma 2) possessing a pterin molybdenum cofactor with a monooxo-monosulfido-type molybdenum center, ( ... | 1995 | 7782304 |
| microbial metabolism of quinoline and related compounds. iv. degradation of isoquinoline by alcaligenes faecalis pa and pseudomonas diminuta 7. | from sewage and soil isoquinoline-degrading organisms were enriched. two strains could be isolated which were able to utilize isoquinoline as sole carbon source. the bacteria were tentatively identified as alcaligenes faecalis and pseudomonas diminuta with respect to their morphological and physiological characters. when growing on isoquinoline both strains excrete a metabolite into the medium which was identified as 1-oxo-1,2-dihydroisoquinoline. alcaligenes faecalis was cultivated in continuou ... | 1990 | 2390217 |
| hydrolysis of tetriso by an enzyme derived from pseudomonas diminuta as a model for the detoxication of o-ethyl s-(2-diisopropylaminoethyl) methylphosphonothiolate (vx). | an enzyme termed organophosphorus hydrolase (oph), derived from pseudomonas diminuta, had been found previously to hydrolyze the powerful acetylcholinesterase (ache) inhibitor o-ethyl s-(2-diisopropylaminoethyl) methylphosphonothiolate (vx). this enzyme has now been shown to be correlated with the loss of ache inhibitory potency (detoxication). oph also hydrolyzed and detoxified the vx analogue, o,o-diisopropyl s-(2-diisopropylaminoethyl) phosphorothiolate (tetriso), also a potent ache inhibitor ... | 1995 | 7887986 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| expression and post-translational processing of a broad-spectrum organophosphorus-neurotoxin-degrading enzyme in insect tissue culture. | a recombinant baculovirus, autographa californica nuclear polyhedrosis virus (acnpv), has been utilized to express the opd (organophosphate-degrading) gene from pseudomonas diminuta in insect tissue-culture cells (sf9) of the fall armyworm (spodoptera frugiperda). the broad-spectrum organophosphate hydrolase (ec 3.1.8.1) encoded by this gene is a member of a general class of enzymes [organophosphate (op) anhydrolases] that include parathion hydrolases, di-isopropyl-fluorophosphatases (dfpases), ... | 1994 | 8031504 |
| transfer and expression of an organophosphate insecticide-degrading gene from pseudomonas in drosophila melanogaster. | the organophosphorus acid hydrolases represent a distinct class of enzymes that catalyze the hydrolysis of a variety of organophosphate substrates, including many insecticides and their structural analogues. the plasmid-borne opd gene of pseudomonas diminuta strain mg specifies an organophosphorus acid hydrolase, a phosphotriesterase, that has been well characterized and can hydrolyze a broad spectrum of insect and mammalian neurotoxins. the in situ functioning of this enzyme in the metabolism o ... | 1990 | 2172975 |
| tumor necrosis factor changes sensitivity of differentiation of mouse leukemia m1 cells by lipopolysaccharide. | a clone of mouse leukemia m1 cells was induced to differentiate by lipopolysaccharide (lps) (lps-sensitive clone) while another clone of the same cells was resistant (lps-resistant clone). lps and lipid a preparations from pseudomonas diminuta and pseudomonas vesicularis were as active as escherichia coli lps in the induction of differentiation of the lps-sensitive clone. synthetic lipid a precursor ia (compound 406), which has no interleukin 1 (il-1)-inducing activity toward monocytes, had stro ... | 1990 | 2163998 |
| characterization of organophosphorus hydrolases and the genetic manipulation of the phosphotriesterase from pseudomonas diminuta. | there are a variety of enzymes which are specifically capable of hydrolyzing organophosphorus esters with different phosphoryl bonds from the typical phosphotriester bonds of common insecticidal neurotoxins (e.g. paraoxon or coumaphos) to the phosphonate-fluoride bonds of chemical warfare agents (e.g. soman or sarin). these enzymes comprise a diverse set of enzymes whose basic architecture and substrate specificities vary dramatically, yet they appear to be ubiquitous throughout nature. the most ... | 1993 | 8393748 |
| [transfer of various markers in lactobacilli during transformation of plasmid dna and joint cultivation]. | a system providing a high frequency genetic transfer of various markers making the reference strain lactobacillus buchneri 1837 resistant to lm, em and fus, able to ferment some carbohydrates and antagonistic against pseudomonas diminuta ccm 2657 was developed. the frequency of the marker transfer during the lactobacilli joint cultivation was 1.5 x 10(-5) = 5.5 x 10(-5) to 1.5 x 10(-4) = 5.5 x 10(-4) which was 3-4 orders of magnitude higher than the marker transfer frequency during transformatio ... | 1990 | 2116781 |
| detection of pseudomonas aeruginosa from ovine fleece washings by pcr amplification of 16s ribosomal rna. | two oligonucleotides were selected from the variable regions of the 16s rrna gene of p. aeruginosa and used as pcr primers for the detection of p. aeruginosa. the specificity of the primers was tested against the following bacterial species; pseudomonas putida, pseudomonas cepacia, xanthamonas maltophilia, pseudomonas mendocina, pseudomonas stutzeri, pseudomonas fluorescens, pseudomonas alcaligenes and pseudomonas diminuta. these primers had a sensitivity of detection of 1 pg of chromosomal dna ... | 1995 | 8604555 |
| limits of diffusion in the hydrolysis of substrates by the phosphotriesterase from pseudomonas diminuta. | the catalytic mechanism for the enzymatic hydrolysis of a series of paraoxon analogues by the phosphotriesterase from pseudomonas diminuta has been determined. the brønsted plots relating the pka of the leaving group to the observed kinetic parameters, vmax and v/km, are both nonlinear. this observation is consistent with a change in the rate-limiting step from chemical to physical events as the pka of the leaving group is decreased. this conclusion is confirmed by the effects of solvent viscosi ... | 1991 | 1649628 |
| pyrimidine base and ribonucleoside catabolic enzyme activities of the pseudomonas diminuta group. | pyrimidine base and ribonucleoside catabolic enzyme activities of the two type strains of the pseudomonas diminuta group were investigated for taxonomic classification purposes. the presence of the pyrimidine salvage enzyme nucleoside hydrolase was indicated in both type strains following thin-layer chromatographic analysis. the presence of the hydrolase was also confirmed by enzyme assay. in addition, the activities of the pyrimidine salvage enzymes dihydropyrimidine dehydrogenase and dihydropy ... | 1992 | 1490615 |
| [rotrac capillary pore membranes for laboratory filtration. ii. bacteria-free filtration]. | because of their special characteristics capillary pore membranes (cpm) are now applied in several branches of separation techniques and analytics. besides applications in particle analytics and microfiltration of different media capillary pore membranes can be used in microorganism separation. it was shown that rotrac cpm can be used for bacteria free (or so called sterile) filtration. acceptable fluxes were reached in separation of pseudomonas diminuta (test species atcc 19146). membranes with ... | 1992 | 1457036 |
| testing and evaluation of off-gas filters for bioreactors by a new bacterial aerosol challenge test method (tbac). | a tno bacterial aerosol challenge (tbac) filter test rig was developed for direct assessment of the effectiveness of bioreactor off-gas filters as an alternative to routinely applied indirect wet integrity testing (it). this tbac test rig is based on bacterial aerosol challenging with pseudomonas diminuta and dual monitoring by laser particle counting (lpc) and andersen microbial sampling (ams) of viable cells. the tbac filter test rig is able to reproduce the various conditions encountered in f ... | 1992 | 1368427 |
| cultural and biochemical characteristics and fatty acid composition of pseudomonas diminuta and pseudomonas vesiculare. | the cultural characteristics, biochemical activity, and cellular fatty acid composition of pseudomonas diminuta and pseudomonas vesiculare provided means for differentiation of these closely related species. broth cultures of p. diminuta showed turbid growth and a distinct surface pellicle after 24 h at 35 c. p. vesiculare had no pellicle, and only light, diffuse growth was observed. all strains of p. vesiculare oxidized maltose and hydrolyzed esculin to varying degrees; p. diminuta was negative ... | 1975 | 1176607 |
| susceptibility of nonfermentative gram-negative bacilli to tobramycin. | there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ... | 1976 | 823278 |
| occurrence of 2,3-diamino-2,3-dideoxy-d-glucose in lipid a from lipopolysaccharide of pseudomonas diminuta. | | 1978 | 745005 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| host factor for coliphage qbeta rna replication as an aid in elucidating phylogenetic relationships: the genus pseudomonas. | host factor (hf) is a heat-stable rna-binding protein required along with qbeta replicase for in vitro transcription of coliphage qbeta rna. we have found that hf activity and antigenicity are conserved among certain gram-negative bacterial species. we examined selected species within the genus pseudomonas for the presence of the hf polypeptide's antigenicity and qbeta rna replication function. while we were unable to detect either of these in pseudomonas diminuta or pseudomonas vesicularis, the ... | 1977 | 411889 |
| glucuronosyl diacylglycerol of pseudomonas diminuta atcc 11568. in vitro biosynthesis from udp-glucuronate and diacylglycerol. | the biosynthesis of glucuronosyl diacylglycerol from udp-glucuronate and diacylglycerol is catalyzed by an enzyme found in both the 34,800 x g supernatant and particulate preparations from disrupted pseudomonas diminuta (atcc 11586). udp-glucuronate served as the glucuronosyl donor and could not be replaced by glucuronic acid, glucuronate-1-phosphate, and a number of nucleotide-linked sugars. the maximum velocity was estimated to be 19 nmol of glucuronosyl diacylglycerol synthesized/h/mg of prot ... | 1977 | 405393 |
| adsorptive retention of pseudomonas diminuta by membrane filters. | | 1979 | 253026 |
| bacteriocuprein superoxide dismutases in pseudomonads. | two new instances of the rare bacteriocuprein form of superoxide dismutase have been discovered in pseudomonas diminuta and p. maltophilia. each species contains a manganese superoxide dismutase as well. eight other strains of pseudomonas and xanthomonas spp. lacked bacteriocupreins and contained either a manganese or an iron superoxide dismutase. native molecular weights and isoelectric points were determined for all these bacterial dismutases. a monospecific polyclonal antibody was prepared ag ... | 1985 | 3997777 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |
| the atrazine catabolism genes atzabc are widespread and highly conserved. | pseudomonas strain adp metabolizes the herbicide atrazine via three enzymatic steps, encoded by the genes atzabc, to yield cyanuric acid, a nitrogen source for many bacteria. here, we show that five geographically distinct atrazine-degrading bacteria contain genes homologous to atza, -b, and -c. the sequence identities of the atz genes from different atrazine-degrading bacteria were greater than 99% in all pairwise comparisons. this differs from bacterial genes involved in the catabolism of othe ... | 1998 | 9537398 |
| enzymatic and nonenzymatic dehydration reactions of l-arogenate. | l-arogenate, an immediate precursor of either l-tyrosine, l-phenylalanine, or both in many microorganisms and plants, may undergo two types of dehydration reactions that yield products of increased stability. under acidic conditions, a facile aromatization attended by loss of the c-4 hydroxyl and the c-1 carboxyl moieties results in quantitative conversion to l-phenylalanine. when aromatization was largely prevented by maintaining ph in the range of 7.5-12, a second dehydration reaction occurred ... | 1985 | 3924095 |
| [efficacy and tolerance of imipenem/cilastatin in the treatment of surgical infections with and without bone involvement]. | imipenem/cilastatin in a doses of 1.5/day was used to treat 31 moderate to severe infections, predominantly soft tissue infections with bone involvement, in 30 surgical patients. a clinical success was achieved in 93% of the patients. in one patient with diabetic gangrene of the foot, imipenem/cilastatin treatment performed as a last resort was not able to prevent amputation. one patient died from his underlying disease while on therapy. all isolates except one pseudomonas diminuta strain regard ... | 1986 | 3759250 |
| dissimilar plasmids isolated from pseudomonas diminuta mg and a flavobacterium sp. (atcc 27551) contain identical opd genes. | the opd (organophosphate-degrading) gene derived from a 43-kilobase-pair plasmid (psm55) of a flavobacterium sp. (atcc 27551) has a sequence identical to that of the plasmid-borne gene of pseudomonas diminuta. hybridization studies with dna fragments obtained by restriction endonuclease digestion of plasmid dnas demonstrated that the identical opd sequences were encoded on dissimilar plasmids from the two sources. | 1988 | 3202637 |
| tumor necrosis factor-inducing activities of lipid a preparations from pseudomonas diminuta and pseudomonas vesicularis. | tumor necrosis factor (tnf)-inducing activities of lipid a preparations from p. diminuta and p. vesicularis, which contain mainly 2 mol of 2,3-diamino-2,3-dideoxy-d-glucose and 1 mol of nonglycosidic phosphate as the backbone component and have partly different fatty acid compositions, were examined. tnf was induced by injecting various lipid a fractions into mice that had previously been sensitized with mycobacterium bovis bcg vaccine. a major component of lipid a of both strains, referred to a ... | 1988 | 3136115 |
| [aerobic bacterial flora of the nasal cavity of rabbits]. | on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ... | 1986 | 3096189 |
| chemical structure of lipid a isolated from flavobacterium meningosepticum lipopolysaccharide. | the chemical structure of the lipid a of the lipopolysaccharide component isolated from flavobacterium meningosepticum ifo 12535 was elucidated. methylation and nuclear magnetic resonance analyses showed that two kinds of hydrophilic backbone exist in the free lipid a: a beta (1-->6)-linked 2-amino-2-deoxy-d-glucose, which is usually present in enterobacterial lipid a's, and a 2-amino-6-o-(2, 3-diamino-2,3-dideoxy-beta-d-glucopyranosyl)-2-deoxy-d-glucose, in a molar ratio of 1.00:0.35. both back ... | 1998 | 9683486 |
| lipids of pseudomonas diminuta. | | 1969 | 5364260 |
| diverse enzymological patterns of phenylalanine biosynthesis in pseudomonads are conserved in parallel with deoxyribonucleic acid homology groupings. | l-tyrosine biosynthesis in nature has proven to be an exceedingly diverse gestalt of variable biochemical routing, cofactor specificity of pathway dehydrogenases, and regulation. a detailed analysis of this enzymological patterning of l-tyrosine biosynthesis formed a basis for the clean separation of five taxa among species currently named pseudomonas, xanthomonas, or alcaligenes (byng et al., j. bacteriol. 144:247-257, 1980). these groupings paralleled taxa established independently by ribosoma ... | 1981 | 7263614 |
| expression of organophosphate hydrolase in the filamentous fungus gliocladium virens. | the broad-spectrum organophosphate hydrolase (oph; ec 3.1.8.1) encoded by the organophosphate-degrading gene (opd) from pseudomonas diminuta mg and flavobacterium sp. atcc 27551 possesses capabilities of both p-o bond hydrolysis (e.g. paraoxon) and p-f bond hydrolysis [e.g. sarin and diisopropylfluorophosphate (dfp)]. in the present study a 9.4-kb plasmid, pcl1, was used to transform the saprophytic fungus gliocladium virens. pcl1 was derived from pjs294 by placing the fungal promoter (prom1) fr ... | 1994 | 7764970 |
| enzymes hydrolyzing organophosphates as potential catalytic scavengers against organophosphate poisoning. | enzymes hydrolyzing organophosphates could be used as catalytic scavengers for treatment of organophosphate poisoning and for decontamination. two organophosphorus hydrolases (oph) were selected: the flavobacterium sp/pseudomonas diminuta phosphotriesterase (pte) and human paraoxonase (hupon). genes encoding these enzymes were cloned and functional recombinant enzymes expressed. pte was expressed in e. coli. natural hupon was purified from human plasma; recombinant hupon was expressed in human e ... | 1998 | 9789837 |
| ratios of carbon isotopes in microbial lipids as an indicator of substrate usage. | the occurrence and abundance of microbial fatty acids have been used for the identification of microorganisms in microbial communities. however, these fatty acids can also be used as indicators of substrate usage. for this, a systematic investigation of the discrimination of the stable carbon isotopes by different microorganisms is necessary. we grew 11 strains representing major bacterial and fungal species with four different isotopically defined carbon sources and determined the isotope ratio ... | 1998 | 9797266 |
| sequence diversity of the opri gene, coding for major outer membrane lipoprotein i, among rrna group i pseudomonads. | the sequence of opri, the gene coding for the major outer membrane lipoprotein i, was determined by pcr sequencing for representatives of 17 species of rrna group i pseudomonads, with a special emphasis on pseudomonas aeruginosa and pseudomonas fluorescens. within the p. aeruginosa species, opri sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. the opri sequences diverged more for the other rrna group i pseudomonads (85 to 91% si ... | 1998 | 9851998 |
| microbial retention characteristics of 0.2-microns-rated nylon membrane filters during filtration of high viscosity fluids at high differential pressure and varied temperatures. | regulatory guidelines for aseptic manufacture of pharmaceuticals recommend validation of sterilizing filters by bacterial challenge under "worst case" conditions. the microbial retention characteristics of a sterilizing grade, 0.2-microns-rated nylon 66 membrane filter were evaluated for filtration of high viscosity fluids. glycerol (80%) was used as the carrier fluid and pseudomonas diminuta atcc 19146 as the challenge organism. testing was carried out at a constant differential pressure of 60 ... | 1995 | 7780747 |
| in situ analysis of phototrophic sulfur bacteria in the chemocline of meromictic lake cadagno (switzerland). | comparative sequence analysis of a 16s rrna gene clone library from the chemocline of the meromictic lake cadagno (switzerland) revealed the presence of a diverse number of phototrophic sulfur bacteria. sequences resembled those of rrna of type strains chromatium okenii dsm169 and amoebobacter purpureus dsm4197, as well as those of four bacteria forming a tight cluster with a. purpureus dsm4197 and lamprocystis roseopersicina dsm229. in situ hybridization with fluorescent (cy3 labeled) oligonucl ... | 1999 | 10049902 |
| discrimination of burkholderia multivorans and burkholderia vietnamiensis from burkholderia cepacia genomovars i, iii, and iv by pcr. | we present a pcr procedure for identification of burkholderia cepacia, burkholderia multivorans, and burkholderia vietnamiensis. 16s and 23s ribosomal dnas (rdnas) of b. multivorans and b. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. specific antisense 16s rdna primers (for b. cepacia, 5'-agc act ccc rcc tct cag-3'; for b. multivorans, 5'-agc act ccc gaa tct ctt-3') and 23s rdna primers (for b. vietnamiensis, ... | 1999 | 10203482 |
| three-dimensional structure of the binuclear metal center of phosphotriesterase. | phosphotriesterase, as isolated from pseudomonas diminuta, is capable of detoxifying widely used pesticides such as paraoxon and parathion and various mammalian acetylcholinesterase inhibitors. the enzyme requires a binuclear metal center for activity. recently, the three-dimensional structure of the apoenzyme was solved (benning et al., 1994) and shown to consist of an alpha/beta-barrel. here we describe the three-dimensional structure of the holoenzyme, reconstituted with cadmium, as determine ... | 1995 | 7794910 |
| classification of pseudomonas diminuta leifson and hugh 1954 and pseudomonas vesicularis büsing, döll, and freytag 1953 in brevundimonas gen. nov. as brevundimonas diminuta comb. nov. and brevundimonas vesicularis comb. nov., respectively. | the taxonomic positions of strains previously assigned to pseudomonas diminuta and pseudomonas vesicularis were investigated by a polyphasic approach. the results of dna-rrna hybridization studies indicated that these two species belong to a separate genus in the alpha subclass (rrna superfamily iv) of the proteobacteria, for which the name brevundimonas is proposed. genus delineation and species delineation were determined by comparing the results of numerical analyses of whole-cell protein pat ... | 1994 | 8068543 |
| stereochemical preferences for chiral substrates by the bacterial phosphotriesterase. | the bacterial phosphotriesterase from pseudomonas diminuta catalyzes the hydrolysis of organophosphate nerve agents such as paraoxon (diethyl p-nitrophenyl phosphate) with a turnover number of approximately 10(4) s(-1). the active site of the enzyme has been shown to be composed of a binuclear zn2+ complex with a bridging hydroxide. the utilization of chiral phosphotriesters has demonstrated that the overall hydrolytic reaction occurs with net inversion of stereochemistry at the phosphorus cente ... | 1999 | 10421456 |
| degradation of nerve gases by clecs and cells: kinetics of heterogenous systems. | we have reported the enzymatic hydrolysis of phosphoro- and phosphonofluoridates and phosphoro- and phosphonothiolates and -thionates by an organophosphorus hydrolase (oph) from pseudomonas diminuta. in screening for other microbial sources of nerve gas hydrolyzing enzymes, it would be convenient, indeed essential, to be able to determine such hydrolyses on intact cells. as a preliminary step to such screening we have measured the hydrolysis of o,o-diisopropyl s-(2-diisopropylaminoethyl) phospho ... | 1999 | 10421481 |
| involvement of the cis/trans isomerase cti in solvent resistance of pseudomonas putida dot-t1e. | pseudomonas putida dot-t1e is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. we have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholipids, mainly cis-oleic acid (c(16:1,9)) and cis-vaccenic acid (c(18:1,11)), in response to solvents. to determine the importance of this cis/trans isomerase for solvent resistance a ... | 1999 | 10482510 |
| capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. | we used capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) analysis of pcr-amplified 16s rrna gene fragments for rapid identification of pseudomonas aeruginosa and other gram-negative nonfermenting bacilli isolated from patients with cystic fibrosis (cf). target sequences were amplified by using forward and reverse primers labeled with various fluorescent dyes. the labeled pcr products were denatured by heating and separated by capillary gel electrophoresis with an autom ... | 1999 | 10488211 |
| purification and characterization of isoquinoline 1-oxidoreductase from pseudomonas diminuta 7, a novel molybdenum-containing hydroxylase. | isoquinoline 1-oxidoreductase, which catalyzes the hydroxylation of isoquinoline to 1-oxo-1,2-dihydroisoquinoline with concomitant reduction of a suitable electron acceptor, was purified from the isoquinoline degrading bacterium pseudomonas diminuta 7 to apparent homogeneity. the native enzyme was a heterodimer with a molecular mass of 95 kda consisting of a 16- and a 80-kda subunit. it contained 0.85 g atom molybdenum, 3.95 g atom iron, 3.9 g atom acid-labile sulfur, 2.1 mol of phosphate, and 1 ... | 1994 | 8157655 |
| structural characterization of the divalent cation sites of bacterial phosphotriesterase by 113cd nmr spectroscopy. | the phosphotriesterase from pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters. the isolated native protein contains zinc, and removal of this metal abolishes the enzymatic activity. reconstitution of the apoenzyme requires 2 mol of cadmium per mol of protein for full catalytic activity. the kcat and km values for the hydrolysis of paraoxon for the cadmium-substituted enzyme are 4300 s-1 and 390 microm, respectively. these values compare favorably with the kinetic constants ... | 1993 | 8396425 |
| quinaldine 4-oxidase from arthrobacter sp. rü61a, a versatile procaryotic molybdenum-containing hydroxylase active towards n-containing heterocyclic compounds and aromatic aldehydes. | quinaldine 4-oxidase from arthrobacter sp. rü61a, an inducible molybdenum-containing hydroxylase, was purified to homogeneity by an optimized five-step procedure. molecular oxygen is proposed as physiological electron acceptor. electrons are also transferred to artificial electron acceptors with e'o > -8 mv. the molybdo-iron/sulfur flavoprotein regiospecifically attacks its n-heterocyclic substrates: isoquinoline and phthalazine are hydroxylated adjacent to the n-heteroatom at cl, whereas quinal ... | 1996 | 8617260 |
| three-dimensional structure of the zinc-containing phosphotriesterase with the bound substrate analog diethyl 4-methylbenzylphosphonate. | phosphotriesterase from pseudomonas diminuta catalyzes the hydrolysis of paraoxon and related acetylcholinesterase inhibitors with rate enhancements that approach 10(12). the enzyme requires a binuclear metal center for activity and as isolated contains 2 equiv of zinc per subunit. here we describe the three-dimensional structure of the zn2+/zn2+-substituted enzyme complexed with the substrate analog diethyl 4-methylbenzylphosphonate. crystals employed in the investigation belonged to the space ... | 1996 | 8634243 |
| interference of pseudomonas strains in the identification of helicobacter pylori. | | 2000 | 10722321 |
| involvement of two plasmids in fenitrothion degradation by burkholderia sp. strain nf100. | a bacterium capable of utilizing fenitrothion (o,o-dimethyl o-4-nitro-m-tolyl phosphorothioate) as a sole carbon source was isolated from fenitrothion-treated soil. this bacterium was characterized taxonomically as being a member of the genus burkholderia and was designated strain nf100. nf100 first hydrolyzed an organophosphate bond of fenitrothion, forming 3-methyl-4-nitrophenol, which was further metabolized to methylhydroquinone. the ability to degrade fenitrothion was found to be encoded on ... | 2000 | 10742273 |
| a mouse kidney- and liver-expressed cdna having homology with a prokaryotic parathion hydrolase (phosphotriesterase)-encoding gene: abnormal expression in injured and polycystic kidneys. | to investigate abnormalities in gene expression associated with cyst formation in polycystic kidney disease, differential cdna library screening was carried out using rna from normal and cystic kidneys of the c57bl/6j-cpk mouse. among a number of genes found to be abnormally expressed was one (cdna clone 56-1) that was significantly underexpressed in cystic kidneys. hybridization analyses revealed that the 56-1 mrna is expressed primarily in kidney and liver, and that the kidney expression begin ... | 1996 | 8654936 |
| development and qualification of a novel virus removal filter for cell culture applications. | commercial bioreactors employing mammalian cell cultures to express biological or pharmaceutical products can become contaminated with adventitious viruses. the high expense of such a contamination can be reduced by passing all gases and fluids feeding the bioreactor through virus inactivation or removal steps, which act as viral barriers around the bioreactor. a novel virus barrier filter has been developed for removing viruses from serum-free cell culture media. this filter removes the 20 nm m ... | 2000 | 10835245 |
| evaluation of recovery filters for use in bacterial retention testing of sterilizing-grade filters. | membrane filters with pore-size ratings of 0.22 microns and 0.45 microns were tested for their ability to recover pseudomonas diminuta atcc 19146 (p. diminuta), the organism typically used in bacterial retention testing of sterilizing-grade membrane filters. for each of the two pore-size ratings, filters of two membrane filter polymer materials, hydrophilic pvdf (millipore durapore) and mixed esters of cellulose, were tested, resulting in an evaluation of four potential recovery filters. the 0.4 ... | 1996 | 8696777 |
| metal-substrate interactions facilitate the catalytic activity of the bacterial phosphotriesterase. | the bacterial phosphotriesterase from pseudomonas diminuta is a zinc metalloenzyme which catalyzes the hydrolysis of a variety of organophosphorus nerve agents with high efficiency. the active site of the enzyme consists of a coupled binuclear metal center embedded within a cluster of histidine residues. potential protein-substrate interactions at the active site were probed by a systematic variation of metal identity, leaving group potential, phosphate host, and amino acid replacement. in order ... | 1996 | 8718883 |
| metabolic characterisation of a novel vanillylmandelate-degrading bacterium. | a newly isolated gram-negative bacterium, possibly brevundimonas diminuta, utilised d,l-vanillylmandelate (d,l-vma) as a sole carbon and energy source. the organism converted d,l-vma to vanillylglyoxylate using a soluble nad-dependent dehydrogenase specific for d-vma and a dye-linked, membrane-associated l-vma dehydrogenase. vanillylglyoxylate was further metabolised by decarboxylation, dehydrogenation and demethylation to protocatechuate. a 4,5-dioxygenase cleaved protocatechuate to 2-hydroxy-4 ... | 1996 | 8824148 |
| reassessment of the phylogenetic position of caulobacter subvibrioides. | determination of the 16s rrna gene sequence of caulobacter subvibrioides atcc 15264t (t = type strain) confirmed that this species is a member of the alpha subclass of the proteobacteria and showed that it is phylogenetically most closely related to the caulobacter group comprising the species caulobacter bacteroides, caulobacter crescentus, and brevundimonas (pseudomonas) diminuta, for which 16s rrna sequences of the type strains are currently available. the closest known relative of strain atc ... | 1997 | 8995825 |
| development of a direct in situ pcr method for detection of specific bacteria in natural environments. | we applied hnpp (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ pcr for the routine detection of specific bacterial cells at the single-cell level. pcr was performed on glass slides with digoxigenin-labeled dutp. the digoxigenin-labeled pcr products were detected with alkaline phosphatase-labeled antidigoxigenin antibody and hnpp which was combined with fast red tr. a bright red fluorescent signal was produced from conversion to hnp (dephosphorylated form) by alkaline p ... | 1998 | 9546190 |
| degradation of organophosphorous nerve agents by enzyme-polymer nanocomposites: efficient biocatalytic materials for personal protection and large-scale detoxification. | the biocatalytic destruction of organophosphates has become an important focus area, as efficient "clean" technologies are sought for chemical weapons decommissioning, counteracting nerve agent attacks, and protecting against organophosphate pesticide poisoning. a novel method is advanced for immobilizing the broad-spectrum enzyme organophosphorous hydrolase (oph) from pseudomonas diminuta, based on the formation of nanocomposite protein-silicone polymers. the resulting materials are highly acti ... | 2000 | 11005922 |
| 16s ribosomal dna sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. | some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16s ribosomal dna (rdna)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. however, as yet, the usefulness of 16s rdna sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. in this study, we evaluated the utility of 1 ... | 2000 | 11015374 |
| phylogeny and identification in situ of nevskia ramosa. | an enrichment of the neuston bacterium nevskia ramosa was investigated by the cultivation-independent rrna approach. n. ramosa was first described by famintzin in 1892 as a rod-shaped, slightly bent bacterium forming typical flat rosettes on the surface of shallow freshwater habitats by unilateral slime formation. pcr in combination with cloning and sequencing was used for retrieving 21 partial and 5 nearly full-length 16s rrna sequences forming three tight clusters. in situ hybridization with r ... | 1998 | 9572969 |
| intergeneric transfer of conjugative and mobilizable plasmids harbored by escherichia coli in the gut of the soil microarthropod folsomia candida (collembola). | the gut of the soil microarthropod folsomia candida provides a habitat for a high density of bacterial cells (t. thimm, a. hoffmann, h. borkott, j. c. munch, and c. c. tebbe, appl. environ. microbiol. 64:2660-2669, 1998). we investigated whether these gut bacteria act as recipients for plasmids from escherichia coli. filter mating with e. coli donor cells and collected feces of f. candida revealed that the broad-host-range conjugative plasmid prp4-luc (prp4 with a luciferase marker gene) transfe ... | 1998 | 9647844 |
| evaluation of the vitek 2 system for rapid identification of medically relevant gram-negative rods. | the new vitek 2 system (biomérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (id-gnb card). of the 845 strains tested, which represented 70 different taxa belonging to either the family enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole r ... | 1998 | 9650942 |
| hydrolysis of phosphodiesters through transformation of the bacterial phosphotriesterase. | the phosphotriesterase from pseudomonas diminuta catalyzes the hydrolysis of a wide array of phosphotriesters and related phosphonates, including organophosphate pesticides and military nerve agents. it has now been shown that this enzyme can also catalyze the hydrolysis of phosphodiesters, albeit at a greatly reduced rate. however, the enzymatic hydrolysis of ethyl-4-nitrophenyl phosphate (compound i) by the wild-type enzyme was >10(8) times faster than the uncatalyzed reaction (kcat = 0.06 s-1 ... | 1998 | 9651332 |
| microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments. | a microcosm enrichment approach was employed to isolate bacteria which are representative of long-term biphenyl-adapted microbial communities. growth of microorganisms was stimulated by incubating soil and sediment samples from polluted and nonpolluted sites with biphenyl crystals. after 6 months, stable population densities between 8 x 10(9) and 2 x 10(11) cfu/ml were established in the microcosms, and a large percentage of the organisms were able to grow on biphenyl-containing minimal medium p ... | 1998 | 9687466 |
| degradation of 1,2,3,4-tetrachlorobenzene by pseudomonas chlororaphis rw71 | pseudomonas chlororaphis rw71 mineralized 1,2,3,4-tetrachlorobenzene, a highly recalcitrant pollutant hitherto not known to be degraded by pure cultures, as a sole source of carbon and energy, thereby releasing stoichiometric amounts of chloride. the transient excretion of tetrachlorocatechol in the early growth phase suggests an initial attack by a dioxygenase to form the corresponding dihydrodiol which rearomatizes to the catechol. the activity of chlorocatechol 1,2-dioxygenase in crude cell e ... | 1998 | 9758802 |