| differences in antibacterial resistance related to differences in cell envelope structure of pseudomonas aeruginosa and pseudomonas cepacia [proceedings]. | | 1978 | 32267 |
| immunological comparison of enzymes of the beta-ketoadipate pathway. | beta-carboxy-cis,cis-muconate lactonizing enzyme and gamma-carboxymuconolactone decarboxylase catalyze sequential reactions in the beta-ketoadipate pathway; the subunit sizes of the enzymes from pseudomonas putida, biotype a, are 40 000 and 13 000, respectively. the cross reaction of antisera prepared against the enzymes was tested with the isofunctional enzymes formed by representatives of other bacterial species. despite the differences in the subunit sizes of the enzymes, the antisera reveale ... | 1976 | 65161 |
| characterization of the fatty acid-sensitive glucose 6-phosphate dehydrogenase from pseudomonas cepacia. | the adenosone 5'-triphosphate-insensitive glucose 6-phosphate dehydrogenase from pseudomonas cepacia has been found to be strongly inhibited by long-chain fatty acids and their acyl coenzyme a esters, suggesting that an important role of this isoenzyme might be to provide reduced nicotinamide adenine dinucleotide phosphate for reductive steps in fatty acid synthesis. the enzyme, which has been redesignated the fatty acid-sensitive glucose 6-phosphate dehydrogenase, has been purified to homogenei ... | 1977 | 72065 |
| isolation of atypical lipopolysaccharides from purified cell walls of pseudomonas cepacia. | wall fragments were prepared from two strains of pseudomonas cepacia and from p. aeruginosa, and their contents of readily extractable lipid, pronase-digestible protein and lipopolysaccharide were measured. lipopolysaccharide extracted from p. cepacia, although biologically active, contained no detectable 2-keto-3-deoxyoctonic acid, but contained phosphate, rhamnose, glucose, heptose and hexosamine in concentrations comparable to those found in p. aeruginosa. | 1979 | 113493 |
| subacute and acute endocarditis due to pseudomonas cepacia in heroin addicts. | five heroin addicts were treated for endocarditis caused by pseudomonas cepacia. two of these infections occurred in patients with no known heart disease whereas the others occurred at sites of previous endocarditis or valve prostheses. infection was indolent in four patients but was associated with shock and skin lesions suggestive of ecthyma gangrenosum in the fifth. after failure of chloramphenicol and kanamycin, all patients were treated with a combination of sulfamethoxazole, trimethoprim a ... | 1975 | 166559 |
| resistance of spheroplasts and whole cells of pseudomonas cepacia to polymyxin b. | sucrose-lysozyme spheroplasts were prepared from two strains of pseudomonas cepacia and tested for susceptibility to polymyxin b and benzalkonium chloride. spheroplasts were more susceptible than whole cells to benzalkonium chloride but not to polymyxin b. disruption of the outer membrane layer was not by itself sufficient to render p. cepacia susceptible to polymyxin b. | 1978 | 213017 |
| [antimicrobial susceptibility patterns of clinical isolates of pseudomonas cepacia (author's transl)]. | the yearly changes of relative frequency of glucose-nonfermentative gram-negative bacilli except for p. aeruginosa isolated from various clinical specimens over the past 5 years were studied. acinetobacter anitratum was the most commonly encountered strain and p. maltophilia and p. putida were also frequently encountered during 1974 through 1977. in 1978, p. cepacia became a significantly predominant strain, which was 44.6% of all nonfermentative gram-negative bacilli excluding p. aeruginosa iso ... | 1979 | 259663 |
| pseudomonas cepacia bacteraemia due to intrinsic contamination of an anaesthetic. bacteriological and serological observations. | in november-december 1977 an epidemic of bacteraemia due to p. cepacia was observed in odense, denmark (nine patients), and in nijmegen, holland (seven patients). all patients recovered. the epidemic was traced to intrinsic contamination of two batches of the anaesthetic fentanyl. all isolates from the patients and from the two batches belonged to the same biotype, had identical sensitivity patterns, and identical antigens. the p. cepacia strain differed from stock strains in being able to grow ... | 1979 | 373379 |
| comparison of the api 20e and corning n/f systems for identification of nonfermentative gram-negative rods. | a total of 231 strains of nonfermenting gram-negative rods were tested on the api 20e system, the corning n/f system, and conventional media. when the results of identification to species were compared, the api system agreed with the conventional system on 69% of the isolates, and the corning system agreed with the conventional system on 79% of the isolates. both kit systems were deficient in identifying pseudomonas cepacia and the more unusual isolates. | 1979 | 389947 |
| resistance plasmids in pseudomonas cepacia 4g9. | pseudomonas cepacia 4g9 utilizes 2-tridecanone as its sole carbon source and has been shown to be resistant to a variety of antibiotics. to ascertain whether any of these characteristics were plasmid mediated, escherichia coli hb101 was transformed with plasmid dna isolated from pseudomonas cepacia 4g9. no 2-tridecanone-utilizing transformants were obtained. tetracycline (tc)- and ampicillin (ap)- resistant transformants were obtained at a low frequency. plasmid deoxyribonucleic acid from antibi ... | 1979 | 391795 |
| pseudomonas species bacteremia caused by contaminated normal human serum albumin. | in may and june 1973, 11 patients on the surgical service at the university of maryland hospital had bacteremia caused by pseudomonas species. seven of the isolates recovered from blood cultures had the same antibiogram (sensitive only to chloramphenicol and tetracycline). ten of the 11 patients were given 25% normal serum albumin (human) shortly before the onset of symptoms. in contrast, only two of seven patients with bacteremia due to psuedomonas aeruginosa in may and june (p =0.013) and only ... | 1977 | 404366 |
| pseudomonas cepacia in 16 non-fatal cases of postoperative bacteremia derived from intrinsic contamination of the anaesthetic fentanyl. clinical and epidemiological observations in denmark and holland. | from nov. 15 to dec. 17, 1977, pseudomonas cepacia was isolated from the blood of 16 patients in odense, denmark, and nijmegen, holland, 2--5 days after an operation with general anaesthesia. the fever started 14--70 h after operation and lasted 2--4 days. all patients recovered. 14/15 patients examined 7--51 days later had agglutinating antibody titres of 400-3,200 against the epidemic strain. ps. cepacia with identical biochemical characters and sensitivity pattern was isolated from unbroken v ... | 1979 | 419367 |
| bacteriocin, plasmid and pectolytic diversity in pseudomonas cepacia of clinical and plant origin. | pseudomonas cepacia strains of plant and clinical origin were compared with the type strains of p. cepacia, p. kingii and p. multivorans. conventional biochemical tests and antibiotic sensitivity patterns supported the previous proposals of synonymy between p. cepacia, p. kingii and p. multivorans. however, bacteriocin production patterns, onion maceration tests and hydrolysis of low ph pectate agar clearly differentiated strains of clinical and plant origin into two distinct groups; these tests ... | 1979 | 430027 |
| branched chain amino acid aminotransferase isoenzymes of pseudomonas cepacia. | pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent branched chain amino acid aminotransferase activity than extracts of glucose-grown bacteria. the increase in enzyme activity was due to the presence of a second aminotransferase not de ... | 1979 | 443990 |
| fourteen-year survival of pseudomonas cepacia in a salts solution preserved with benzalkonium chloride. | a strain of pseudomonas cepacia that survived for 14 years (1963 to 1977) as a contaminant in an inorganic salt solution which contained commercial 0.05% benzalkonium chloride (cbc) as an antimicrobial preservative, was compared to a recent clinical isolate of p. cepacia. ammonium acetate was present in the concentrated stock cbc solution, and served as a carbon and nitrogen source for growth when carried over into the salts solution with the cbc. the isolate's resistance to pure benzalkonium ch ... | 1979 | 453827 |
| pseudomonas cepacia mutants blocked in the direct oxidative pathway of glucose degradation. | glucose dehydrogenase-deficient strains of pseudomonas cepacia grew normally with glucose as carbon source, indicating that the direct pathway of glucose oxidation does not play an essential role in this bacterium. | 1979 | 457605 |
| [pseudomonas cepacia bacteremia caused by intravenously administered fentanyl]. | | 1979 | 460472 |
| [the microflora in cosmetics and non-sterile pharmaceuticals, and the microbiological standards for these products. i. a case of waterborne contamination of a hand-lotion (author's transl)]. | a current case of a dynamic contamination of hand lotion with pseudomonas cepacia is described. the ps. cepacia occurred in the hand-lotion as a pure culture in a number of 10(4) bacteria per ml hand-lotion. the contaminant, originating from the tap-water, formed a preliminary reservoir in the deionizer, passed the sterile-filter and entered via the sterile water into the production system, where it formed a secondary reservoir in the top of one of the mixing units. | 1979 | 461120 |
| [clinical and bacteriological studies on a case with endocarditis due to pseudomonas cepacia (author's transl)]. | | 1979 | 491262 |
| [pseudomonas cepacia hospital infections; uncertainties and experiences with an epidemiological investigation (author's transl)]. | during a hospital outbreak of pseudomonas cepacia a traditional epidemiological investigation was proved to be misleading. suppression of the suspected antiseptic did not stop the epidemics. a further investigation proved that it was due to the intravenous injection of contamineted anesthetic vials. fever, chills, lack of hemodynamic disorders and of metastatic absceses characterize the clinical state of the infected patients. the removal of the catheters for perfusion was sometimes necessary to ... | 1979 | 493080 |
| hydroxy amino acid metabolism in pseudomonas cepacia: role of l-serine deaminase in dissimilation of serine, glycine, and threonine. | growth of pseudomonas cepacia (p. multivorans) on serine depended upon induction of a previously undescribed l-serine deaminase distinct from threonine deaminase. formation of the enzyme was induced during growth on serine, glycine, or threonine. the induction pattern reflected a role of the enzyme in catabolism of these three amino acids. both threonine and glycine supported growth of serine auxotrophs and were presumably converted to serine and pyruvate in the course of their degradation. muta ... | 1979 | 500557 |
| pseudomonas cepacia resistance to antibacterials. | reproducible growth rates of pseudomonas cepacia were obtained. p. cepacia had a markedly different resistance pattern to single and combined antibacterials from that characteristic of pseudomonas aeruginosa. benzalkonium and chlorhexidine were more active against log phase p. cepacia than against log phase p. aeruginosa, but polymyxin b sulfate was inactive against log phase p. cepacia at all concentrations tested (smaller than or equal to 16 units/ml). antagonism of antibacterial activity betw ... | 1979 | 512895 |
| 3,3,3-trifluoro-2-aminoisobutyrate: a mechanism-based inhibitor of pseudomonas cepacia alpha-dialkylamino acid transaminase. | | 1979 | 518585 |
| response of pseudomonas cepacia to beta-lactam antibiotics: utilization of penicillin g as the carbon source. | pseudomonas cepacia utilized penicillin g as the sole source of carbon and energy. we report here an unexplained correlation between lysine auxotrophy and beta-lactamase deficiency, resulting in loss of capacity to utilize penicillin. | 1979 | 533766 |
| [pseudomonas cepacia (author's transl)]. | | 1977 | 559148 |
| 4,9-dihydroxyphenazine-1,6-dicarboxylic acid dimethylester and the 'missing link' in phenazine biosynthesis. | 4,9-dihydroxyphenazine-1,6-dicarboxylic acid dimethylester, the ester form of a proposed 'missing link' in the biosynthesis of phenazines, has been isolated from a strain of pseudomonas cepacia. | 1978 | 632805 |
| [on pseudomonas cepacia isolated from clinical samples (author's transl)]. | | 1978 | 702804 |
| [pseudomonas cepacia bacteremia caused by intravenously administered fentanyl]. | | 1978 | 703876 |
| characterization of bacteriophage cp1, an organic solvent sensitive phage associated with pseudomonas cepacia. | pseudomonas cepacia strain 249 has been found to harbor an organic solvent sensitive phage, cp1, which is active on other p. cepacia strains. the efficiency of plating of cp1 was dependent upon the strain on which it was propagated and the strain used as indicator, implying the operation of host restriction and modification systems in certain of the strains. strain 383 which was used routinely for propagation of cp1 appears to lack such systems. to obtain high-titer lysates it was important to a ... | 1978 | 743648 |
| [pseudomonas cepacia septicemia in 2 infants]. | | 1978 | 756991 |
| inhibition of pseudomonas strains in two soft contact lens soaking solutions. | ten pseudomonas aeruginosa and six pseudomonas cepacia strains recently isolated from infected patients were tested for susceptibility to the antimicrobial activity of two commercially-available soft contact lens soaking solutions. no strain showed evidence of viability after two hours but survivors were found in both solutions after one-half hour and in one solution after one hour. the solution most effective in inhibiting the pseudomonas strains contained chlorhexidine and disodium edetate but ... | 1976 | 824034 |
| pseudomonas cepacia endocarditis and ecthyma gangrenosum. | a case of right-sided pseudomonas cepacia endocarditis in a heroin addict is presented in which septic cutaneous vasculitis (ecthyma gangrenosum) is a prominent feature. ecthyma gangrenosum, most commonly associated with sepsis due to p aeruginosa, has not been previously described with p cepacia septicemia. | 1977 | 836696 |
| lung abscess due to pseudomonas cepacia. | a diabetic patient with pneumonia of unspecified origin developed a lung abscess after therapy with ultrasonic nebulization. the etiologic organism was identified as pseudomonas cepacia. investigation determined the source of the organism to be the reservoir of the ultrasonic nebulizer, to which the patient was directly exposed through removal of the bottom of the disposable medication cup. when this organism is isolated a nosocomial source of infection should be suspected. | 1977 | 855960 |
| pseudomonas cepacia septic arthritis due to intra-articular injections of methylprednisolone. | | 1977 | 861878 |
| a novel ketone monooxygenase from pseudomonas cepacia. purification and properties. | a ketone monooxygenase was purified from cells of pseudomonas cepacia grown on 2-tridecanone as sole carbon source. enzyme stability is maintained by the addition of ethanol, edta, and dithiothreitol. stoichiometric studies show that for 1 mol of undecyl acetate formed, 1 mol of o2 is consumed and 1 mol of nadph is oxidized. the monooxygenase, purified to homogeneity, has a molecular weight of approximately 123,000 and consists of two equal subunits with molecular weights of 55,000. the enzyme c ... | 1977 | 925012 |
| pseudomonas cepacia pneumonia in a child with chronic granulomatous disease and selective iga deficiency. | a 6 1/2 year-old boy with chronic granulomatous disease (cgd) and selective iga deficiency developed a chronic progressive pneumonia which failed to respond to several conventional combinations of antimicrobial therapy. on lung biopsy, pseudomonas cepacia was obtained in pure culture, sensitive to chloramphenicol, tetracycline, kanamycin and nalidixic acid. with specific therapy, he slowly recovered. p. cepacia has not been previously described as a cause of persistent pneumonia in immunodeficie ... | 1976 | 937004 |
| pressure transducers as a source of bacteremia after open heart surgery. report of an outbreak and guidelines for prevention. | during four weeks in 1974, eight (26 percent) of 31 intensive care unit patients who had undergone open-heart surgery developed symptomatic pseudomonas cepacia bacteremia in the intensive care unit one to three days after the open-heart surgery. an investigation demonstrated that operating room pressure transducers were being contaminated during cleaning with a detergent that contained p cepacia at the rate of 10(4) organisms per milliliter and that the organisms were transmitted to patients aft ... | 1976 | 971602 |
| nosocomial pseudobacteremia. positive blood cultures due to contaminated benzalkonium antiseptic. | pseudomonas cepacia or enterobacter species or both were isolated from blood cultures of 79 patients in a community hospital between april 1971 and march 1972. no common exposures other than venipuncture correlated with positive blood cultures. pseudomonas cepacia, enterobacter, and other gram-negative enteric bacteria were cultured from aqueous benzalkonium chloride used for skin antisepsis prior to ordinary and blood culture venipuncture. contamination of blood cultures by organisms from the a ... | 1976 | 989858 |
| contaminated aqueous benzalkonium chloride. an unnecessary hospital infection hazard. | during january and february 1975, nine patients on a single ward of a rural tennessee hospital unexpectedly developed sepsis. the aseptic technique employed in the management of intravenous infusions was implicated. pseudomonas cepacia was recovered from the following: bloodstream, inuse intravenous infusions and the antiseptic, aqueous benzalkonium chloride. the outbreak again calls attention to the infection risk associated with the use of this product. selection of less hazardous antiseptics ... | 1976 | 989860 |
| preliminary clinical trial with amikacin in chronic recurrent gram-negative bacterial infections refractory to other antimicrobial agents. | eleven patients with 16 infections due to enterobacteriaceae or pseudomonas that were in most cases refractory to treatment with cephalosporins, kanamycin, gentamicin, tobramycin, carbenicillin, or ampicillin (administered singly or in combinations) received 1 g of amikacin daily by the intramuscular route (10 patients) or 0.4 g daily (one patient with renal insufficiency). the average duration of treatment was eight days. at the beginning of treatment, 18 pathogens were sensitive to amikacin at ... | 1976 | 993631 |
| treating pseudomonas cepacia meningitis with trimethoprim-sulfamethoxazole. | an infant with neonatal meningitis caused by pseudomonas cepacia responded promptly to treatment with trimethoprim-sulfamethoxazole (bactrim) after other abtibiotics had failed. pseudomonas cepacia has proven to be resistant to most of the commonly used antibiotics. | 1976 | 998580 |
| pseudomonas cepacia as contaminant of propamidine disinfectants. | | 1976 | 1000272 |
| sternal osteomyelitis and mediastinitis after open-heart operation: pathogenesis and prevention. | sternal osteomyelitis and mediastinitis caused by pseudomonas cepacia developed in a patient undergoing coronary artery bypass two weeks after the operation. p. cepacia bacteremia from a contaminated pressure transducer had preceded and probably caused the chest infection. while other authors have suggested that postoperative sternal osteomyelitis and mediastinitis result from local wound contamination, this case suggests the importance of bacteremia as a cause of such gram-negative infections. ... | 1976 | 1083719 |
| intracellular bactericidal activity of leukocytes in whole blood for the diagnosis of chronic granulomatous disease of childhood. | a simplified method for the assay fo intracellular bactericidal activity of leukocytes in whole blood was developed. providing that serum-resistant organisms are studied, the method can be used with gram-negative baccilli as well as with staphylococcus aureus. the abnormalities characteristic of patients with chronic granulomatous disease of childhood and their mothers can be readily detected by this technique. by this means an abnormality in intracellular killing of pseudomonas cepacia in a pat ... | 1975 | 1092771 |
| recurrent pneumonitis due to pseudomonas cepacia. an unexpected phagocyte dysfunction. | | 1975 | 1114810 |
| differentiation between pseudomonas cepacia and pseudomonas pseudomallei in clinical bacteriology. | if only conventional laboratory tests are used, the widely occurring species pseudomonas cepacia may, due to its variability, sometimes be difficult to differentiate from the dangerous pathogen pseudomonas pseudomallei. in a comparative study using fresh isolates it is shown that animal inoculation seems to be a useful additional method in the differentiation. also the determination of the ability to utilize selected carbon sources such as starch, hydroxybenzoate and uracil is helpful in reachin ... | 1975 | 1155112 |
| in vitro susceptibility of pseudomanas cepacia and pseudomonas maltophilia to trimethoprim and trimethoprim-sulfamethoxazole. | our earlier studies had shown that the two pseudomonads, pseudomonas cepacia and pseudomonas maltophilia, were organisms that were highly resistant to most antibiotics. the present study was undertaken to determine the susceptibility of these bacteria to trimethoprim and the trimethoprim-sulfamethoxazole combination which has been used with apparent success in treating infections caused by these pseudomonads. all 51 strains of p. cepacia were inhibited by 2 mug or less of trimethoprim per ml, wh ... | 1975 | 1155926 |
| association of pseudomonas cepacia with chronic granulomatous disease. | pseudomonas cepacia was recovered from a number of infected sites in three patients with chronic granulomatous disease of childhood. the organisms were identified on the basis of their oxidative utilization of a variety of carbohydrates and their positive beta-galactosidase and oxidase activities. they were resistant to most antimicrobial agents and moderately susceptible to chloramphenicol. peripheral blood leukocytes isolated from two siblings with chronic granulomatous disease, including one ... | 1975 | 1176612 |
| photoreactivation of pseudomonas cepacia after ultraviolet exposure: a potential source of contamination in ultraviolet-treated waters. | cells of a naturally occurring strain of pseudomonas cepacia grown in distilled water were exposed to ultraviolet radiation. irradiated samples incubated on membrane filters or in fluid recovery media in the absence of light showed no evidence of dark repair mechanisms. when samples were exposed to fluorescent light ranging from 50 to 950 foot candles (538 to 10,222 lux) of illumination, apparent photo-induced repair of ultraviolet injury resulted in 1- to 4-log increases in viable cells recover ... | 1975 | 1176614 |
| an epidemic in a coronary care unit caused by pseudomonas species. | five patients in a coronary care unit were involved in an epidemic caused by a nonfermenting gram-negative bacillus, presumptively identified by our laboratory as pseudomonas cepacia. all medications administered intravenously to these patients were cultured. because morphine was the only such medication common to the treatment of all the patients involved, a vial used in the unit at that time was cultured, as were two previously-entered vials of saline solution and several sterile vials of dist ... | 1975 | 1190129 |
| septicaemia from prolonged intravenous infusions. | four cases of septicaemia in children were traced to contaminated intravenous infusions and volume control sets. in each case pseudomonas cepacia was isolated from multiple blood cultures and from intravenous fluid within the volume control set. the first patient died of septicaemia after a long and complicated postoperative period. the other three patients received appropriate antibiotics after removal of the contaminated intravenous sets and they recovered within 2 weeks. | 1975 | 1211961 |
| hospital infections with pigmented water bacteria. | two types of cultures normally expected to be sterile, i.e., urine and blood, were chosen for review at a large university hospital. the bacteria isolated from urine cultures during 1966-70 and from blood cultures from 1966-73 are listed. two organisms, flavobacterium meningosepticum and pseudomonas cepacia, were selected for more detailed study and review. the antibiotic susceptibility patterns for these two organisms are presented. | 1975 | 1236618 |
| identification of pigmented gram negative bacilli. | identification of chromogenic gram-negative bacilli on the basis of pigment production as a taxonomic criterion is unreliable. because of this, a wide range of morphological, cultural and biochemical characteristics of bacteria in this category was examined. the salient features found to be useful for identification of pigmented bacilli, including flavobacterium, xanthomonas, the fluorescent pseudomonads, pseudomonas cepacia, p. stutzeri, p. maltophilia, p. putrefaciens, and group ve, are review ... | 1975 | 1236622 |
| pseudomonas cepacia in an intensive care nursery. | a small epidemic of pseudomonas cepacia infection in an intensive care nursery was associated with contaminated, distilled water. this appeared to have occurred because of a leak in the distiller, contaminating its effluent. the distilled water was not subsequently sterilized, nor were the bottles used to distribute it. the epidemic was promptly terminated by substitution of sterile, distilled water distributed in sterile containers. both professional and nonprofessional hospital personnel faile ... | 1976 | 1250657 |
| pseudomonas cepacia strains isolated from water reservoirs of unheated nebulizers. | pseudomonas cepacia strains were isolated from the water reservoirs of unheated nebulizers in a hospital setting. the isolates were characterized by morphology, biochemical tests, and antibiotic susceptibility patterns. an interesting feature of these organisms was their capacity for sustained multiplication in either doubly deionized or doubly distilled water as well as 5% dextrose or 0.9% saline. they could not multiply in doubly deionized and then doubly distilled water or in any of several p ... | 1976 | 1254703 |
| resistance of a strain of pseudomonas cepacia to esters of p-hydroxybenzoic acid. | cells of a strain of pseudomonas cepacia were isolated from an oil-in-water emulsion containing methyl and propyl p-hydroxybenzoates (methylparaben and propylparaben) as preservative additives. this strain demonstrated the ability to destroy these additives, to utilize the propyl ester as sole carbon source, and to hydrolyze the methyl ester. when the isolate was grown on eugon agar, exposure to the methyl ester killed 99.9% of the inoculum, but the surviving cells grew logarithmically. on the o ... | 1976 | 1275493 |
| inhibition of rat alveolar macrophage phagocytic function by a pseudomonas cepacia lipase. | the effects of purified pseudomonas cepacia lipase on rat pulmonary alveolar function and morphology were examined. lipase (2.5-20 micrograms/ml) adversely effected the phagocytic function of rat pulmonary alveolar macrophages in a dose-dependent manner. the lipase itself was not directly cytotoxic to these cells. alveolar macrophages, in the absence of lipase, phagocytosed c. 35% of a given population of opsonised p. cepacia in 30 min when the ratio of bacteria:phagocyte was 10:1. phagocytosis ... | 1992 | 1279176 |
| crystallization and preliminary x-ray crystallographic analysis of lipase from pseudomonas cepacia. | large crystals of lipase from pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. they grow within two weeks to typical dimensions of 1.0 mm x 0.5 mm x 0.3 mm. the crystals belong to the monoclinic space group p2(1), with unit cell parameters a = 84.91 a, b = 47.33 a, c = 86.00 a, and beta = 116.09 degrees. and they diffract to about 1.6 a upon exposure to synchroton x-rays. x-ray data have been collected to 2.2 a bragg s ... | 1992 | 1279182 |
| salicylate-inducible antibiotic resistance in pseudomonas cepacia associated with absence of a pore-forming outer membrane protein. | the most common mechanism of antibiotic resistance in multiply resistant pseudomonas cepacia is decreased porin-mediated outer membrane permeability. in some gram-negative organisms this form of antibiotic resistance can be induced by growth in the presence of weak acids, such as salicylates, which suppress porin synthesis. to determine the effects of salicylates on outer membrane permeability of p. cepacia, a susceptible laboratory strain, 249-2, was grown in 10 mm sodium salicylate. antibiotic ... | 1992 | 1280056 |
| resonance raman studies of rieske-type proteins. | resonance raman (rr) spectra are reported for the [2fe-2s] rieske protein from thermus thermophilus (trp) and phthalate dioxygenase from pseudomonas cepacia (pdo) as a function of ph and excitation wavelength. depolarization ratio measurements are presented for the rr spectra of spinach ferredoxin (sfd), trp, and pdo at 74 k. by comparison with previously published rr spectra of sfd, we suggest reasonable assignments for the spectra of trp and pdo. the spectra of pdo exhibit virtually no ph depe ... | 1992 | 1280165 |
| community-acquired bacteremic pseudomonas cepacia pneumonia in an immunocompetent host. | | 1992 | 1280168 |
| phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2fe-2s]. | phthalate dioxygenase reductase (pdr) is a prototypical iron-sulfur flavoprotein (36 kilodaltons) that utilizes flavin mononucleotide (fmn) to mediate electron transfer from the two-electron donor, reduced nicotinamide adenine nucleotide (nadh), to the one-electron acceptor, [2fe-2s]. the crystal structure of oxidized pdr from pseudomonas cepacia has been analyzed at 2.0 angstrom resolution resolution; reduced pdr and pyridine nucleotide complexes have been analyzed at 2.7 angstrom resolution. n ... | 1992 | 1280857 |
| production and purification of salicylate monooxygenase from pseudomonas cepacia atcc 29351. | salicylate monooxygenase (ec: 1.14.13.1) has been produced and purified from pseudomonas cepacia atcc 29351 which has the ability to utilise salicylate as a sole carbon source. the bacterium was grown on a defined medium containing 2% (w/v) casamino acids and 0.15% (w/v) yeast extract at 25 degrees c; salicylate monooxygenase production was induced by the presence of up to 0.7% (w/v) sodium salicylate, to a level of approximately 2% of the soluble cell protein. the enzyme was purified over 50-fo ... | 1992 | 1281025 |
| growth and survival of pseudomonas cepacia dbo1 (pro101) in soil amended with 2,4-dichlorophenoxyacetic acid. | the 2,4-dichlorophenoxyacetic acid (2,4-d) degrading pseudomonad, pseudomonas cepacia dbo1(pro101), was inoculated at approximately 10(7) cfu/g into sterile and non-sterile soil amended with 0, 5 or 500 ppm 2,4-d and the survival of the strain was studied for a period of 44 days. in general, the strain survived best in sterile soil. when the sterile soil was amended with 2,4-d, the strain survived at a significantly higher level than in non-amended sterile soil. in non-sterile soil either non-am ... | 1991 | 1282055 |
| mineralization of 2,4-dichlorophenoxyacetic acid (2,4-d) in soil inoculated with pseudomonas cepacia dbo1(pro101), alcaligenes eutrophus aeo106(pro101) and alcaligenes eutrophus jmp134(pjp4): effects of inoculation level and substrate concentration. | mineralization of 2,4-dichlorophenoxyacetic acid (2,4-d) by two alcaligenes eutrophus strains and one pseudomonas cepacia strain containing the 2,4-d degrading plasmids pjp4 or pro101 (= pjp4::tn1721) was tested in 50 g (wet wt) samples of non-sterile soil. mineralization was measured as 14c-co2 evolved during degradation of uniformly-ring-labelled 14c-2,4-d. when the strains were inoculated to a level of approximately 10(8) cfu/g soil, between 20 and 45% of the added 2,4-d (0.05 ppm, 10 ppm or ... | 1991 | 1282056 |
| selection of a pseudomonas cepacia strain constitutive for the degradation of trichloroethylene. | tn5 insertion mutants of pseudomonas cepacia g4 that were unable to degrade trichloroethylene (tce), toluene, or phenol or to transform m-trifluoromethyl phenol (tfmp) to 7,7,7-trifluoro-2-hydroxy-6-oxo-2,4-heptadienoic acid (tfha) were produced. spontaneous reversion to growth on phenol or toluene as the sole source of carbon was observed in one mutant strain, g4 5223, at a frequency of approximately 1 x 10(-4) per generation. one such revertant, g4 5223-pr1, metabolized tfmp to tfha and degrad ... | 1992 | 1282314 |
| the use of detergent-based aqueous two-phase systems for the isolation of extracellular proteins: purification of a lipase from pseudomonas cepacia. | the partitioning of a variety of extracellular lipases, both pro- and eucaryotic, in detergent-based aqueous two-phase systems was examined. the results revealed that all procaryotic lipases showed a clear preference for the detergent-rich coacervate phase. in contrast, all eucaryotic lipases were significantly excluded from this phase, most probably caused by their glycosylation. the potential of such detergent-based systems for the isolation of extracellular lipases directly from cell-free cul ... | 1992 | 1282322 |
| purification and characterization of a novel thermostable lipase from pseudomonas cepacia. | a thermostable lipase from pseudomonas cepacia has been purified to homogeneity as judged by sds-page and isoelectric focusing. the purification included treatment of the culture supernatant with acrinol, hydrophobic interaction chromatography, and gel filtration. the enzyme was a monomeric protein with m(r) of 36,500 and pi of 5.1. the optimal ph at 50 degrees c and optimal temperature at ph 6.5 were 5.5-6.5 and 55-60 degrees c, respectively, when olive oil was used as the substrate. simple tri ... | 1992 | 1282513 |
| effect of polysaccharide interactions on antibiotic susceptibility of pseudomonas aeruginosa. | the relative viscosity of pseudomonas aeruginosa alginate was shown to increase markedly when combined with mucin, ca2+ ions and the exopolysaccharide from pseudomonas cepacia. the presence of such a heterodisperse polysaccharide solution significantly reduced the diffusion and hence antimicrobial activity of tobramycin and to a lesser extent ciprofloxacin against ps. aeruginosa by factors of 90 and 2.5-fold respectively over a 5 h incubation period. the clinical implications of these results ar ... | 1992 | 1283390 |
| significant factors in the disinfection and sterilization of flexible endoscopes. | many nosocomial infection outbreaks have been linked to improper disinfection of the flexible endoscopes used in hospitals and clinics. the objective of this study was to evaluate the efficacy of scope disinfection with glutaraldehyde and hydrogen peroxide in manual and mechanical protocols. | 1992 | 1283507 |
| post-antibiotic effect of ceftazidime, ciprofloxacin, imipenem, piperacillin and tobramycin for pseudomonas cepacia. | the post-antibiotic effects (pae) of ceftazidime, ciprofloxacin, imipenem, piperacillin and tobramycin were studied for ten strains of pseudomonas cepacia isolated from patients with cystic fibrosis. antibiotic concentrations used for exposure were either the mic of each agent for the sensitive isolates or the recommended sensitivity breakpoint concentrations for the resistant isolates. after 2 h of exposure, cultures were rapidly diluted 1000-fold to eliminate the antibiotic. out of the ten iso ... | 1992 | 1283606 |
| the effects of purified 25-kda lipase from a clinical isolate of pseudomonas cepacia in the lungs of rats. | nanogram quantities of a 25-kda lipase purified from culture supernatants of pseudomonas cepacia 90ee, a sputum isolate from a cystic fibrosis (cf) patient, were placed in the lungs of healthy rats. the resulting pathological changes included large amounts of proteinaceous exudate, the accumulation of polymorphonuclear leukocytes and red blood cells, and disorganization of alveolar structure. pseudomonas cepacia 90ee immobilized in agar beads was also placed in the lungs of rats in a model of ch ... | 1992 | 1283726 |
| antibacterial properties of investigational, new, and commonly used antibiotics against isolates of pseudomonas cepacia in michigan. | microdilution antimicrobial susceptibility testing was performed with 73 isolates of pseudomonas cepacia collected from the sputum of patients throughout michigan with cystic fibrosis. susceptibility testing was done using new and investigational antibiotics (loracarbef, cefixime, cefpirome, desacetyl-cefotaxime, cefpodoxime, cefmetazole, cefepime, cefprozil, and fleroxacin) and commonly used antibiotics (ceftazidime, mezlocillin, piperacillin, ciprofloxacin, tobramycin, and amikacin). ceftazidi ... | 1992 | 1283733 |
| proposal of burkholderia gen. nov. and transfer of seven species of the genus pseudomonas homology group ii to the new genus, with the type species burkholderia cepacia (palleroni and holmes 1981) comb. nov. | based on the 16s rrna sequences, dna-dna homology values, cellular lipid and fatty acid composition, and phenotypic characteristics, a new genus burkholderia is proposed for the rna homology group ii of genus pseudomonas. seven species in this group were transferred to the new genus. thus seven new combinations, burkholderia cepacia (palleroni and holmes 1981), burkholderia mallei (zopf 1885), burkholderia pseudomallei (whitmore 1913), burkholderia caryophylli (burkholder 1942), burkholderia gla ... | 1992 | 1283774 |
| low incidence of pulmonary pseudomonas cepacia infection in danish cystic fibrosis patients. | | 1992 | 1283959 |
| in vitro activity of sparfloxacin compared with those of five other quinolones. | the in vitro activity of sparfloxacin, a new difluorinated quinolone, was evaluated against 857 gram-positive and gram-negative clinical isolates and compared with those of ciprofloxacin, norfloxacin, ofloxacin, fleroxacin, and lomefloxacin. the mic of sparfloxacin for 90% of the members of the family enterobacteriaceae tested was 0.5 microgram/ml (range, 0.06 to 4.0 micrograms/ml); only for members of the genera serratia, citrobacter, and providencia were mics above 1 microgram/ml. some 90% of ... | 1992 | 1320362 |
| sequence of isrm4 from rhizobium meliloti strain gr4. | isrm4, an is-like sequence structurally similar to pseudomonas cepacia insertion element is402, was identified by sequence analysis. this 933-bp element carries 17-bp putative terminal inverted repeats with five mismatches and a putative direct target duplication of 3 bp. | 1992 | 1327970 |
| purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from pseudomonas cepacia 2cbs. | the two components of the inducible 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs were purified to homogeneity. yellow component b is a monomer (mr, 37,500) with nadh-acceptor reductase activity. ferricyanide, 2,6-dichlorophenol indophenol, and cytochrome c acted as electron acceptors. component b was identified as an iron-sulfur flavoprotein containing 0.8 mol of flavin adenine dinucleotide, 1.7 mol of iron, and 1.7 mol of acid-labile sulfide per mol of enzyme. the isoelectric po ... | 1992 | 1370284 |
| molecular cloning and characterization of the fusaric acid-resistance gene from pseudomonas cepacia. | fusaric acid-resistance genes (fus) were isolated from pseudomonas cepacia. the nucleotides of the 5437 base pairs containing the fus genes were sequenced. | 1991 | 1370369 |
| pseudomonas cepacia infection in cystic fibrosis. | | 1992 | 1370564 |
| hepatic pathology of chronic granulomatous disease of childhood. | we reviewed the hepatic pathology of seven cases of chronic granulomatous disease of childhood. all patients were male, with an age range of 5 to 41 years. hepatic biopsy with drainage or wedge resection was performed in five cases to remove abscesses. autopsy was performed in three cases. presentation was typical of infection (fever, leukocytosis) with an elevated serum alkaline phosphatase level. histologically, the most consistent feature was the presence of foamy macrophages that contained a ... | 1992 | 1370879 |
| binding of pseudomonas cepacia to normal human intestinal mucin and respiratory mucin from patients with cystic fibrosis. | although not as prevalent as pseudomonas aeruginosa, pseudomonas cepacia is another opportunistic pathogen which colonizes the lungs of at least some patients with cystic fibrosis. a subgroup of these patients exhibits the "cepacia syndrome", i.e., a rapid clinical deterioration and death within one year. to investigate potential early sites of bacterial attachment, we have measured the specific binding of p. cepacia isolates from cystic fibrosis (cf) sputa to both cf and non-cf mucins purified ... | 1992 | 1371125 |
| degradation of 2-methylbenzoic acid by pseudomonas cepacia mb2. | we report the isolation of pseudomonas cepacia mb2, believed to be the first microorganism to utilize 2-methylbenzoic acid as the sole carbon source. its growth range included all mono- and dimethylbenzoates (with the exception of 2,5- and 2,6-dimethylbenzoates) and 3-chloro-2-methylbenzoate (but not 4- or 5-chloro-2-methylbenzoate) but not chlorobenzoates lacking a methyl group. 2-chlorobenzoate, 3-chlorobenzoate, and 2,3-, 2,4-, and 3,4-dichlorobenzoates inhibited growth of mb2 on 2-methylbenz ... | 1992 | 1371658 |
| efficacy of copper and silver ions with iodine in the inactivation of pseudomonas cepacia. | alternatives to chlorination of water have been sought for reasons which include trihalomethane formation, possible bacterial regrowth, the high concentrations of chlorine required in certain circumstances, and the taste, odour and bodily irritation in chlorine-treated water. electrolytically generated cu and ag ions at low levels, in addition to very low chlorine concentrations, have been suggested as an alternative to routine chlorination. we have examined the combination of cu and ag ions wit ... | 1992 | 1371772 |
| controlled study of pseudomonas cepacia and pseudomonas maltophilia in cystic fibrosis. | in a retrospective study, children with cystic fibrosis who were colonised with pseudomonas cepacia were compared with a control group who were colonised with pseudomonas maltophilia. out of 216 children with cystic fibrosis seen between 1983 and 1990, p cepacia was recovered from 13 (median age at colonisation 12.2 years) and p maltophilia from 23 (median age at first colonisation 6.1 years), and both organisms were recovered in five cases. with the exception of two patients with p cepacia in w ... | 1992 | 1371914 |
| impact of cf summer camp. | in two consecutive years, patients with cystic fibrosis were studied at the beginning and end of a nine-day summer camp program to assess the program's effects on weight gain and pulmonary function. the camp experience includes daily exercise and a high-protein and high-fat diet. there were a total of 58 children between 6 and 12 years of age (42 different patients) and 10 adult counselors from 19 to 30 years of age (eight different patients). on the first and eighth days patients were weighed, ... | 1992 | 1372198 |
| identification of the mucin-binding adhesin of pseudomonas cepacia isolated from patients with cystic fibrosis. | in previous experiments, we have shown that isolates of pseudomonas cepacia from sputa of patients with cystic fibrosis (cf), particularly those with severe lung infection, exhibited specific binding to purified respiratory or intestinal mucins (u. sajjan, m. corey, m. karmali, and j. forstner, j. clin. invest. 89:648-656, 1992). the present report describes the identification of the adhesin as a protein located on fimbriae of mucin-binding p. cepacia. from a total of 53 isolates available (from ... | 1992 | 1372295 |
| isolation of a novel siderophore from pseudomonas cepacia. | a novel iron-binding compound was identified in ethyl acetate extracts of the supernates from pseudomonas cepacia cultures. this compound, named azurechelin, was produced by 88% of p. cepacia strains isolated from the respiratory tract. production of azurechelin was regulated by the iron concentration in the culture medium. azurechelin enhanced the growth of p. cepacia in a medium containing transferrin 200 mg/l. azurechelin released iron from transferrin in an equilibrium dialysis assay, sugges ... | 1992 | 1372361 |
| fimbriation of pseudomonas cepacia. | fimbriae (pili) on the surface of bacteria have been suggested to facilitate adherence to mucosal epithelial surfaces. three pseudomonas cepacia cystic fibrosis isolates were screened for their ability to agglutinate erythrocytes (ha), a characteristic of some fimbrial types. one strain, designated pc103, was ha+, while another, pc109, was ha-. a fimbriated (f+) ha+ derivative of pc109 (pc2(13)) was selected by repeated erythrocyte adsorption. the two ha+ strains were shown by transmission elect ... | 1992 | 1373402 |
| temocillin and cystic fibrosis: outcome of intravenous administration in patients infected with pseudomonas cepacia. | twelve courses of intravenous temocillin were given in combination with an intravenous aminoglycoside to five patients with cystic fibrosis (cf) for pulmonary exacerbations associated with pseudomonas cepacia. all patients were infected concurrently with pseudomonas aeruginosa in addition to p. cepacia. improvement occurred after six of seven courses given to three patients in which temocillin was used as first-line therapy and following three of five courses given to two patients after failure ... | 1992 | 1375595 |
| pseudomonas cepacia--more than a harmless commensal? | | 1992 | 1375968 |
| molecular biology of the 2-haloacid halidohydrolase iva from pseudomonas cepacia mba4. | the structural gene (hdl iva) for the pseudomonas cepacia mba4 2-haloacid halidohydrolase iva (hdl iva) was isolated on a 1.6 kb fragment of ps. cepacia mba4 chromosomal dna. the recombinant halidohydrolase was expressed in escherichia coli and pseudomonas putida and the structural gene was subcloned on to the tac expression vector pbtac1. high-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding t ... | 1992 | 1376111 |
| rapid differentiation of pseudomonas pseudomallei from pseudomonas cepacia. | the minitek disc system was utilized for the differentiation of pseudomonas pseudomallei, the causative agent of melioidosis, from ps. cepacia. the system was simple to use, inexpensive, and furnished rapid, clear-cut test results after 4 h. this procedure is suitable for differentiating soil bacteria presumptively identified as ps. pseudomallei, ps. cepacia or flavobacteria, and for the rapid confirmation of the presumptive identification of either ps. pseudomallei or ps. cepacia obtained by co ... | 1992 | 1376128 |
| infections and pseudoinfections due to povidone-iodine solution contaminated with pseudomonas cepacia. | in 1989 we investigated the first instance of pseudomonas cepacia infections due to intrinsic contamination of a povidone-iodine product. six patients in a texas pediatric facility had p. cepacia infection or pseudoinfection (three, peritonitis; one, pseudoperitonitis; and two, pseudobacteremia). epidemiological studies showed one risk factor for infection of peritoneal fluid with p. cepacia: performance of peritoneal dialysis in the dialysis unit with use of one lot of povidone-iodine later fou ... | 1992 | 1376156 |
| transposon tn5-259 mutagenesis of pseudomonas cepacia to isolate mutants deficient in antifungal activity. | transposon tn5-259 was inserted into the chromosome of pseudomonas cepacia by mating with an escherichia coli strain harboring a self-mobilizable, temperature-sensitive plasmid, pme12. data from southern blots and auxotroph analyses indicated that a single copy of the transposon was inserted in several places into the chromosome of p. cepacia. among 1500 tn5-259 transconjugants, only one mutant was found to be defective in the production of an antifungal compound, pyrrolnitrin. in addition, this ... | 1992 | 1377094 |
| pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling the disease. | in a field experiment, pseudomonas cepacia j82rif and j51rif increased sunflower emergence in the presence of the fungus sclerotinia sclerotiorum. pyrrolnitrin, aminopyrrolnitrin, and monochloroaminopyrrolnitrin were isolated from j82 and identified by using thin-layer chromatography, high-performance liquid chromatography, and electron impact-mass, uv, and infrared spectroscopy. in growth chamber experiments, two antibiosis-negative mutants were not different from the parent strain in protectin ... | 1992 | 1377900 |
| nucleotide sequence of a 2 kb plasmid from pseudomonas cepacia implicated in the degradation of phenylcarbamate herbicides. | the complete nucleotide sequence of a very small plasmid whose presence and level in pseudomonas cepacia have been linked to herbicide resistance is presented. the structural features of the plasmid are discussed. | 1992 | 1378325 |
| pseudomonas cepacia pulmonary infection in adults with cystic fibrosis: is nosocomial acquisition occurring? | ribotyping of 25 isolates of pseudomonas cepacia taken from the sputum of 21 adults with cystic fibrosis (cf) who were registered at the royal brompton hospital between 1987 and 1991, revealed that seven patients (33.3%) shared strains of a similar ribotype pattern with others, while 14 patients (63.7%) harboured strains unique to each individual. constancy of sputum strain carriage was seen in two of three patients sampled twice over a 3-month period. although no evidence for patient-to-patient ... | 1992 | 1379274 |
| detection of micro-organisms in soil after in situ hybridization with rrna-targeted, fluorescently labelled oligonucleotides. | rrna sequences were used as targets for synthetic oligonucleotides labelled with the fluorescent dye tetramethylrhodamine isothiocyanate (tritc) for in situ hybridizations to detect micro-organisms directly in soils that have different contents of soil minerals and organic material. introduced pseudomonas aeruginosa cells were directly fixed in soils and applied to slides after separation of large soil minerals only. remaining soil minerals (clay minerals) and organic material (up to 8%) did not ... | 1992 | 1379623 |
| molecular epidemiology of pseudomonas cepacia determined by polymerase chain reaction ribotyping. | traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal dna with rrna. although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. as an alternative, polymorphisms were sought in the 16s-23s spacer regions of bacterial rrna genes by use of the polymerase chain reaction (pcr). chromosomal dna from isolates of pseudomonas cepacia was used as a template in the pcr wi ... | 1992 | 1380010 |