| the structural genes encoding co dehydrogenase subunits (cox l, m and s) in pseudomonas carboxydovorans om5 reside on plasmid phcg3 and are, with the exception of streptomyces thermoautotrophicus, conserved in carboxydotrophic bacteria. | employing deoxyoligonucleotide probes and southern hybridizations, we have examined in carboxydotrophic bacteria the localization on the genome of genes encoding the large, medium and small subunits of co dehydrogenase (coxl, m and s, respectively). in pseudomonas carboxydovorans om5 coxl, m and s were identified on the plasmid phcg3; they were absent on the chromosome. this was evident from positive hybridizations with plasmid dna of the wild-type strain om5 and the absence of hybridizations wi ... | 1992 | 1510563 |
| studies by e.p.r. spectroscopy of carbon monoxide oxidases from pseudomonas carboxydovorans and pseudomonas carboxydohydrogena. | e.p.r. spectra were obtained at 8-120 k for carbon monoxide oxidases isolated from the carboxydotrophic bacteria pseudomonas carboxydovorans and pseudomonas carboxydohydrogena. spectra from the two enzymes are extremely similar to one another. under appropriate conditions each enzyme shows signals from mo(v) atoms in two different chemical environments, as well as showing signals from two distinct iron-sulphur centres, presumed to be [2fe-2s] clusters, and weak fadh x free-radical signals. param ... | 1983 | 6309136 |
| cloning and molecular characterization of the genes for carbon monoxide dehydrogenase and localization of molybdopterin, flavin adenine dinucleotide, and iron-sulfur centers in the enzyme of hydrogenophaga pseudoflava. | carbon monoxide dehydrogenases (co-dh) are the enzymes responsible for the oxidation of co to carbon dioxide in carboxydobacteria and consist of three nonidentical subunits containing molybdopterin flavin adenine dinucleotide (fad), and two different iron-sulfur clusters (o. meyer, k. frunzke, d. gadkari, s. jacobitz, i. hugendieck, and m. kraut, fems microbiol. rev. 87:253-260, 1990). the three structural genes of co-dh in hydrogenophaga pseudoflava were cloned and characterized. the genes were ... | 1999 | 10482497 |
| phylogenetic affiliation of the pseudomonads based on 16s rrna sequence. | the broad and vague phenotypic definition allowed the genus pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus pseudomonas. the 16s rrna sequences of 128 valid and invalid pseudomonas species, which included almost valid species of the genus pseudomonas listed in the approved lists of bacterial names, were obtained: sequences of 59 species were de ... | 2000 | 10939664 |
| identification of trans- and cis-control elements involved in regulation of the carbon monoxide dehydrogenase genes in mycobacterium sp. strain jc1 dsm 3803. | the cutr gene was identified 314 bp upstream of the divergently oriented cutb1c1a1 operon encoding carbon monoxide (co) dehydrogenase in mycobacterium sp. strain jc1. its deduced product was composed of 320 amino acid residues with a calculated molecular mass of 34.1 kda and exhibits a basal sequence similarity to the regulatory proteins belonging to the lysr family. using a cutr deletion mutant, it was demonstrated that cutr is required for the efficient utilization of co by mycobacterium sp. s ... | 2010 | 20511503 |
| [autrophic growth of seliberia carboxydohydrogena during oxidation of hydrogen and carbon monoxide]. | | 2015 | 4643928 |
| recent advances in petroleum microbiology. | recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. the physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. new molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystem ... | 2003 | 14665675 |
| [characteristics of proteins synthesized by hydrogen-oxidizing microorganisms]. | the study was conducted to determine the biological value of proteins synthesized by hydrogen-oxidizing microorganisms--the hydrogen bacteria alcaligenes eutrophus z1 and ralstonia eutropha b5786 and the co-resistant strain of carboxydobacterium seliberia carboxydohydrogena z1062. based on a number of significant parameters characterizing the biological value of a product, the proteins of hydrogen-oxidizing microorganisms have been found to occupy an intermediate position between traditional ani ... | 2010 | 21261071 |
| correlating carbon monoxide oxidation with cox genes in the abundant marine roseobacter clade. | the marine roseobacter clade (mrc) is a numerically and biogeochemically significant component of the bacterioplankton. annotation of multiple mrc genomes has revealed that an abundance of carbon monoxide dehydrogenase (codh) cox genes are present, subsequently implying a role for the mrc in marine co cycling. the cox genes fall into two distinct forms based on sequence analysis of the coxl gene; forms i and ii. the two forms are unevenly distributed across the mrc genomes. most (18/29) of the m ... | 2010 | 21068776 |
| correlating carbon monoxide oxidation with cox genes in the abundant marine roseobacter clade. | the marine roseobacter clade (mrc) is a numerically and biogeochemically significant component of the bacterioplankton. annotation of multiple mrc genomes has revealed that an abundance of carbon monoxide dehydrogenase (codh) cox genes are present, subsequently implying a role for the mrc in marine co cycling. the cox genes fall into two distinct forms based on sequence analysis of the coxl gene; forms i and ii. the two forms are unevenly distributed across the mrc genomes. most (18/29) of the m ... | 2010 | 21068776 |
| unexpected diversity of bacteria capable of carbon monoxide oxidation in a coastal marine environment, and contribution of the roseobacter-associated clade to total co oxidation. | the species diversity, phylogenetic affiliations, and physiological activity rates of carbon monoxide-oxidizing microorganisms were investigated, using new isolates from surface waters collected from the coast of new england and type strains from established collections. a direct isolation method allowed the simultaneous recovery of organisms with different growth rates and nutritional requirements and the identification of marine microorganisms that oxidize co at an environmentally relevant con ... | 2006 | 16517644 |
| role of carboxydobacteria in consumption of atmospheric carbon monoxide by soil. | the carbon monoxide consumption rates of the carboxydobacteria pseudomonas (seliberia) carboxydohydrogena, p. carboxydovorans, and p. carboxydoflava were measured at high (50%) and low (0.5 mul liter) mixing ratios of co in air. co was only consumed when the bacteria had been grown under co-autotrophic conditions. as an exception, p. carboxydoflava consumed co also after heterotrophic growth on pyruvate. at low cell densities the co consumption rates measured at low co mixing ratios were similar ... | 1981 | 16345821 |
| removal of odorous sulphur-containing gases by a new isolate from activated sludge. | biological treatment of odorous sulphur-containing compounds is attracting attention due to its benign eco-friendliness, energy-savings and low operating costs. as the biological treatment efficiency of dimethylsulphide (dms) reported was often low and variable, selection of useful dms-degrading microorganisms is of importance for the enhancement of the biological deodorizing process. this paper reports the successful isolation of a dms-degrading bacterium from activated sludge, using the enrich ... | 2004 | 15484773 |
| molecular analysis of carbon monoxide-oxidizing bacteria associated with recent hawaiian volcanic deposits. | genomic dna extracts from four sites at kilauea volcano were used as templates for pcr amplification of the large subunit (coxl) of aerobic carbon monoxide dehydrogenase. the sites included a 42-year-old tephra deposit, a 108-year-old lava flow, a 212-year-old partially vegetated ash-and-tephra deposit, and an approximately 300-year-old forest. pcr primers amplified coxl sequences from the omp clade of co oxidizers, which includes isolates such as oligotropha carboxidovorans, mycobacterium tuber ... | 2004 | 15240307 |
| carbon monoxide dehydrogenase activity in bradyrhizobium japonicum. | bradyrhizobium japonicum strain 110spc4 was capable of chemolithoautotrophic growth with carbon monoxide (co) as a sole energy and carbon source under aerobic conditions. the enzyme carbon monoxide dehydrogenase (codh; ec 1.2.99.2) has been purified 21-fold, with a yield of 16% and a specific activity of 58 nmol of co oxidized/min/mg of protein, by a procedure that involved differential ultracentrifugation, anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration ... | 2000 | 10788353 |
| purification and characterization of a manganese-containing superoxide dismutase from a carboxydobacterium, pseudomonas carboxydohydrogena. | superoxide dismutase from pseudomonas carboxydohydrogena, a carboxydobacterium, grown on carbon monoxide was purified 37.6-fold in seven steps to homogeneity, with a yield of 1.4%. the final specific activity was 2,396 units per mg protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. the molecular weight of the native enzyme was determined to be 42,500. sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 21,70 ... | 1997 | 9509413 |
| purification and some properties of ribulose 1,5-bisphosphate carboxylases/oxygenases from acinetobacter sp. strain jc1 and hydrogenophaga pseudoflava. | ribulose 1,5-bisphosphate carboxylases/oxygenases (rubiscos) of two carboxydobacteria, acinetobacter sp. strain jc1 and hydrogenophaga pseudoflava, grown on carbon monoxide were purified and partially characterized. rubisco of acinetobacter sp. jc1 was purified 5-fold in eight steps to homogeneity, with a yield of 1.6%. the final specific activity of the purified enzyme was 39.5 nmol co2 incorporated per min per mg protein. the molecular weight of the native enzyme was determined to be 520,000. ... | 1997 | 9264026 |
| [lipid composition of carboxydobacteria]. | the composition of lipids was studied in three strains of carboxydobacteria, viz. seliberia carboxydohydrogena z-1062, pseudomonas gazotropha z-1156 and comamonas compransoris z-1155. the content of lipids extracted with an alcohol--chloroform mixture varied in these strains from 9 to 13.2%. the main classes were phospholipids (from 62.6 to 77.2% of the overall lipid content). the major phospholipids were phosphatidyl ethanolamine and phosphatidyl choline. moreover, the strains z-1062 and z-1156 ... | 1980 | 7442569 |
| [metabolism of organic nitrogen compounds by carboxydobacteria]. | the capability to hydrolyze and assimilate creatinine, uric and hippuric acids as sources of nitrogen, carbon and energy was studied in comamonas compransoris z-1155, seliberia carboxydohydrogena z-1062 and pseudomonas gazotropha z-1156. the organisms effectively hydrolyzed these compounds and assimilated their nitrogen under the conditions of autotrophic growth. the carboxydobacteria were also capable of growing in media containing creatinine, uric and hippuric acids and assimilated these compo ... | 1981 | 7219217 |
| purification and some properties of carbon monoxide dehydrogenase from pseudomonas carboxydohydrogena. | a soluble yellow co dehydrogenase from co-autotrophically grown cells of pseudomonas carboxydohydrogena was purified 35-fold in seven steps to better than 95% homogeneity with a yield of 30%. the final specific activity was 180 mumol of acceptor reduced per min per mg of protein as determined by an assay based on the co-dependent reduction of thionin. methyl viologen, nicotinamide adenine dinucleotide (phosphate), flavin mononucleotide, and flavin adenine dinucleotide were not reduced by the enz ... | 1981 | 6895515 |
| molybdopterin in carbon monoxide oxidase from carboxydotrophic bacteria. | the carbon monoxide oxidases (coxs) purified from the carboxydotrophic bacteria pseudomonas carboxydohydrogena and pseudomonas carboxydoflava were found to be molybdenum hydroxylases, identical in cofactor composition and spectral properties to the recently characterized enzyme from pseudomonas carboxydovorans (o. meyer, j. biol. chem. 257:1333-1341, 1982). all three enzymes exhibited a cofactor composition of two flavin adenine dinucleotides, two molybdenums, eight irons and eight labile sulfid ... | 1984 | 6582059 |
| homology and distribution of co dehydrogenase structural genes in carboxydotrophic bacteria. | the 17 (s), 30 (m) and 87 kda (l) subunits of co dehydrogenases from the co-oxidizing bacteria pseudomonas carboxydoflava, pseudomonas carboxydohydrogena and pseudomonas carboxydovorans om5 were isolated and purified. the n-terminal sequences of same subunits from different bacteria showed distinct homologies. dot blot hybridization employing oligonucleotide probes derived from the sequences of the s-subunit of p. carboxydovorans om5 and the m-subunit of p. carboxydohydrogena and dna of the plas ... | 1989 | 2818128 |
| purification and some properties of carbon monoxide dehydrogenase from acinetobacter sp. strain jc1 dsm 3803. | a brown carbon monoxide dehydrogenase from co-autotrophically grown cells of acinetobacter sp. strain jc1, which is unstable outside the cells, was purified 80-fold in seven steps to better than 95% homogeneity, with a yield of 44% in the presence of the stabilizing agents iodoacetamide (1 mm) and ammonium sulfate (100 mm). the final specific activity was 474 mumol of acceptor reduced per min per mg of protein as determined by an assay based on the co-dependent reduction of thionin. methyl violo ... | 1989 | 2536687 |
| carbon monoxide dehydrogenase inhibitor in cell extracts of pseudomonas carboxydovorans. | extracts of heterotrophically grown cells of pseudomonas carboxydovorans were found to contain an inhibitor of carbon monoxide dehydrogenase (co-dh). the inhibitor activity was not detected in co-autotrophically grown cells. the inhibitor was extremely stable to heat treatment based on the extent of inhibition of co-dh activity. the extent of inhibition was proportional to the amount of cell extract added to the reaction mixture. the inhibition was independent of a prior incubation period of the ... | 1990 | 2106505 |
| [effects of the nitrogen nutrition conditions on the growth and protein synthesis of carboxydobacteria]. | the rate of growth of bacterial strains oxidizing carbon monoxide (pseudomonas gazotropha z-1156, comamonas compransoris z-1155, and seliberia carboxydohydrogena z--1062) was studied as a function of the concentration of nh4cl in the medium. the bacteria could grow on media containing various nitrogen sources (nh4cl, kno3, co(nh2)2). changes in the amino acid content and biochemical composition of the biomass were studied during growth of the bacteria on these media. the biological value of prot ... | 1979 | 423812 |
| [autotrophic cultivation of carboxide bacteria in continuous cultures]. | the carboxydobacterium seliberia carboxydohydrogena z-1062 was grown under the conditions of continuous cultivation in the atmosphere of hydrogen, oxygen, carbon dioxide, and carbon monoxide. the concentration of the bacterial cells in the chemostat depended on the specific growth rate at the inhibition with co. the biochemical composition of the carboxydobacterial biomass, the rate of utilization of the gaseous mixture by the culture, and the economic coefficients were studied when the flow rat ... | 2013 | 7392993 |
| ubiquinones (coenzyme q) in hydrogen oxidizing bacteria. | the isoprenoid quinone content and composition of several strains of the following hydrogen oxidizing bacteria were studied: xanthobacter autotrophics, xanthobacter flavus, paracoccus denitrificans, pseudomonas pseudoflava, p. carboxydovorans, p. carboxydo-hydrogena. all strains studied contained ubiquinone (uq) in concentrations between 0.7 and 2.8 μmol/g bacterial dry weight. no menaquinones were found. uq of all strains (except one) contained side chain lengths of 10isoprenoid units (uq-10), ... | 1983 | 23194590 |
| [growth of seliberia carboxydohydrogena carboxy bacteria with an altered composition of the gas mixture]. | the growth and gas exchange of seliberia carboxydohydrogena z-1062 were studied in the regime of turbidostat when the conditions of gaseous nutrition were changed: a decrease in hydrogen concentration and an increase in carbon monoxide concentration, growth on two carbon sources (co+co2) and on two energy sources (h2+co). the inhibition of the bacterial growth by co was expressed in a decrease of the specific growth rate and in the reduced effectiveness of using a gaseous substrate. when the con ... | 2013 | 6417461 |
| effect of carbon monoxide on metabolism and ultrastructure of carboxydobacteria. | growth of seliberia carboxydohydrogena was inhibited by co at 10 to 40% (v/v), resulting in increased substrate utilization and enhanced synthesis of cytochromes and cyclopropane and saturated fatty acids. the bacteria showed increased formation of new membrane structures, with pronounced folding of their cell walls. | 1993 | 24419937 |
| synthesis of poly(3-hydroxybutyrate) by the autotrophic co-oxidizing bacterium seliberia carboxydohydrogena z-1062. | the present study addresses growth parameters and physiological and biochemical characteristics of the aerobic co-oxidizing carboxydobacterium seliberia carboxydohydrogena z-1062. poly(3-hydroxybutyrate) yields were investigated in experiments with limiting concentrations of mineral nutrients (nitrogen or sulfur or nitrogen and sulfur) in batch culture of s. carboxydohydrogena z-1062 grown on gas mixtures consisting of co(2), o(2), h(2), and co. co concentrations of 10, 20, and 30 % v/v did not ... | 2015 | 26254039 |
| [pathways of assimilation of carbon oxides in carboxydobacteria seliberia carboxydohydrogena and achromobacter carboxydus]. | assimilation products of 14c-bicarbonate and carbon-14c oxide were studied in two carboxydobacteria seliberia carboxydohydrogena and achromobacter carboxydus which differed in their ability for chemolithoautrophous growth in the presence of hydrogen. the dynamics and composition of labeled products formed upon assimilation of 14c-bicarbonate in the presence of unlabeled carbon oxide by the two organisms, the composition of products formed upon assimilation of 14co by suspensions of s. carboxydoh ... | 1977 | 600091 |
| electron transport system of an aerobic carbon monoxide-oxidizing bacterium. | experiments with crude extracts of pseudomonas carboxydohydrogena revealed that a quinone is necessary for co oxidation, and that cytochromes of the a, b, and c types and functional terminal oxidase(s) are found in cells grown on co as the sole source of carbon and energy. co dehydrogenase delivers electrons to the electron transport system at the level of quinone, and nicotinamide adenine dinucleotide (phosphate) is not involved in co oxidation. | 1981 | 6273386 |