evidence for a periplasmic location in comamonas terrigena of the inducible tyrosine sulfate sulfohydrolase.the location of the inducible and constitutive forms of tyrosine sulfate sulfohydrolase in comamonas terrigena was investigated by subjecting resting cells to osmotic shock and to treatment with lysozyme in the presence of edta. the bulk of this enzyme present in induced cells was released by these procedures, suggesting that the induced form is cell wall associated. the constitutive form present in noninduced cells was not released under these conditions. evidence was also presented which sugge ...1979110429
[content of nitrogen bases in the dna of carboxide bacteria].the composition of nitrogen bases was determined in total dna of live strains of carboxide bacteria belonging to the genera pseudomonas, comamonas, seliberia (agrobacterium), achromobacter. considerate differences in the nucleotide composition of dna of the studied bacteria confirm that they indeed belong to different genera. at the same time, the content of gc pairs in dna of these bacteria is within the range found for the corresponding genera by other authors.1977882021
nucleotide and derived amino acid sequences of the major porin of comamonas acidovorans and comparison of porin primary structures.the dna sequence of the gene which codes for the major outer membrane porin (omp32) of comamonas acidovorans has been determined. the structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. comparisons with amino acid sequences of outer membrane proteins and porins from several other members of the class proteobacteria and of the chlamydia trachomatis porin and the neurospora crassa mitochondrial porin revealed a motif of eight r ...19911848840
ocular infections associated with comamonas acidovorans.comamonas acidovorans (pseudomonas acidovorans) is a ubiquitous gram-negative rod. although generally considered nonpathogenic, we found c. acidovorans to be associated with six cases of ocular infections. the organism was the only isolate in three cases, whereas an association of other organisms was present in three cases. the multiple resistance patterns of these strains to antibiotic susceptibility testing emphasizes the need for culturing ocular infections. we recommend the identification an ...19911882921
the three-dimensional structure of the regular surface protein of comamonas acidovorans derived from native outer membranes and reconstituted two-dimensional crystals.the three-dimensional structure of the regular surface protein (p4 symmetry, lattice constant a = b = 10.5 nm) of comamonas acidovorans has been determined to a resolution of about 1.5 nm by means of electron microscopy and image processing. three-dimensional reconstructions were performed using native outer membranes and artificial two-dimensional crystals of the surface protein, which was selectively solubilized by deoxycholate and recrystallized on carbon films. the two-fold symmetric morphol ...19911719335
modelling of mixed chemostat cultures of an aerobic bacterium, comamonas testosteroni, and an anaerobic bacterium, veillonella alcalescens: comparison with experimental data.a mathematical model of mixed chemostat cultures of the obligately aerobic bacterium comamonas testosteroni and the anaerobic bacterium veillonella alcalescens grown under dual limitation of l-lactate and oxygen was constructed. the model was based on michaelis-menten-type kinetics for the consumption of substrates, with noncompetitive inhibition of v. alcalescens by o2. the growth characteristics of the aerobic and anaerobic organisms were determined experimentally with pure cultures of the ind ...19921622213
dye-linked dehydrogenase activities for formate and formate esters in amycolatopsis methanolica. characterization of a molybdoprotein enzyme active with formate esters and aldehydes.cell-free extracts of methanol-grown amycolatopsis methanolica contain dye-linked dehydrogenase activities for formate and methyl formate. fractionation of the extracts revealed that the (unstable) activity for formate resides in membrane particles, while that for methyl formate belongs to a soluble enzyme that was purified and characterized. the enzyme, indicated as formate-ester dehydrogenase, appeared to be a molybdoprotein (4 fe, 3 or 4 s, 1 mo and 1 fad were found for each enzyme molecule), ...19921597191
purification and properties of guanosine triphosphate cyclohydrolase and a stimulating protein from comamonas sp. (atcc 11299a). 19744415400
the phenylalanine-binding protein of comamonas sp. (atcc 11299a). 19715132661
pseudomonas infections in patients with aids and aids-related complex.we identified and reviewed retrospectively all the cases of infection by pseudomonas and related genera in patients with aids and aids-related complex (arc) who were hospitalized at our institution over a 36-month period. we recorded 48 episodes of infection in 34 of 355 patients with aids, and in two of 73 patients with arc: 25 pneumonias (9 community-acquired and 16 of nosocomial origin). 20 urinary tract infections, two soft tissue infections and one sepsis. in 14 of 16 patients with nosocomi ...19921588272
two-dimensional crystallization of a bacterial surface protein on lipid vesicles under controlled conditions.the solubilized surface protein of the gram-negative bacterium comamonas acidovorans was reconstituted on lipid vesicles by means of controlled dialysis. to this end, a multichamber dialysis apparatus was built which allows one to control the temperature and the dialysis rate, to apply various temperatures or buffer systems and sample conditions in a single experiment, and to monitor the turbidity of the sample by means of light scattering. the reconstitution conditions were optimized such that ...19921540688
chemical and cultural characterization of cdc group wo-1, a weakly oxidative gram-negative group of organisms isolated from clinical sources.ninety-six strains of weakly oxidative gram-negative rods isolated primarily from clinical specimens form a distinct group that has been designated centers for disease control (cdc) group wo-1 (wo stands for weak oxidizer). the phenotypic characteristics of cdc group wo-1 were most similar to those of comamonas acidovorans, pseudomonas mallei, and cdc pink coccoid group iii. the wo-1 group can be differentiated from c. acidovorans by the oxidation of glucose (often weak and sometimes delayed), m ...19921537895
the effect of bovine serum albumin on the measurement of the activity of highly purified comamonas phenylalanine hydroxylase. 19705529638
novel degradative pathway of 4-nitrobenzoate in comamonas acidovorans nba-10.a comamonas acidovorans strain, designated nba-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. when grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. in cell extracts with nadph present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-ni ...19921527502
further studies on the substrate specificity and inhibition of the stereospecific cs2 secondary alkylsulphohydrolase of comamonas terrigena.a series of d-alkan-2-yl sulphate esters (c(7)-c(14)) were prepared by sulphation of the resolved parent alcohols by a method that entails complete retention of configuration. these sulphate esters were tested as substrates for the stereospecific cs2 secondary alkylsulphohydrolase of comamonas terrigena. v(max.) reached a maximum with the c(9) compound, whereas logk(m) decreased linearly as the alkyl-chain length was increased from c(7) to c(14). a parallel series of l-alkan-2-yl sulphates was a ...19806263248
topology of the anion-selective porin omp32 from comamonas proteolysis experiments were performed with outer membranes from comamonas acidovorans to probe the topology of its major protein component, the anion-selective porin omp32. proteinase k treatment above a critical temperature of 42 degrees c cleaved the surface-exposed regions of the porin, yielding membrane-embedded fragments which were separated by sds polyacrylamide gel electrophoresis or reversed phase chromatography. the identification of the proteinase k-sensitive sites was perform ...19921373289
identification of xenobiotic-degrading isolates from the beta subclass of the proteobacteria by a polyphasic approach including 16s rrna partial sequencing.nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. the presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone q-8) allowed allocation of these organisms to the beta subclass of the proteobacteria. on the basis of the results of additional characterization experiments (i.e., api 20ne tests, determinations of soluble protein patterns, and dna-dn ...19921371062
secondary alkylsulphatases in a strain of comamonas terrigena.the occurrence in a strain of comamonas terrigena of secondary alkylsulphatase activity towards potassium decan-5-yl sulphate is reported. a number of cell-washing and osmotic-shock procedures for releasing bacterial exocytoplasmic enzymes were ineffective in releasing this activity. primary alkylsulphatases are not present in the organism, nor can their formation be induced under a wide variety of experimental conditions tested.19751180908
[disc-electrophoresis of the protein spectra of representatives of the genera aeromonas, comamonas and pseudomonas]. 1976961260
a novel mechanism of enzymic ester hydrolysis. inversion of configuration and carbon-oxygen bond cleavage by secondary alkylsulphohydrolases from detergent-degrading micro-organisms.the hydrolysis was studied of potassium (+)-octan-2-yl sulphate by two analogous, optically stereospecific, secondary alkylsulphohydrolases purified from two detergent-degrading micro-organisms, comamonas terrigena and pseudomonas c12b. polarimetry studies have shown that (+)-octan-2-yl sulphate prepared from (+)-octan-2-ol is hydrolysed by both enzymes to yield (-)-octan-2-ol. this inversion of configuration implies that the enzymes are catalysing the scission of the c-o bond of the c-o-s linka ...1977921766
[attempt to establish genome similarity between alcaligenes faecalis and several gram-negative bacteria using the method of molecular hybridization of dna to dna].the technique of dna-dna molecular hybridization was used to demonstrate the absence of genome similarity between alcaligenes faecalis and gram-negative bacteria belonging to the genera pseudomonas, enterobacterium, comamonas producing alkalis, and vibrions. some similarity in the polynucleotide sequences was found between the dna of the reper strain of alcaligenes faecalis 45 (5--10% of homology) and the dna of the culture belonging to the achromobacter genus; therefore, the two genera may belo ...1977870806
purification, properties and cellular localization of the stereospecific cs2 secondary alkylsulphohydrolase of comamonas terrigena.the availability of homogeneous samples of the potassium salts of l- and d-octan-2-yl sulphate has enabled the separation of the optically stereospecific cs1 and cs2 secondary alkysulphohydrolases from extracts of cells of comamonas terrigena. the cs2 enzyme was purified to homogeneity, and an initial study was made of its general properties, specificity, cellular localization and relationship to the cs1 enzyme. the cs2 enzyme has a molecular weight of approx. 250000 and a subunit size of approx ...1977603633
preparation and characterization of substrates suitable for the study of stereospecific secondary alkylsulphohydrolases of detergent-degrading micro-organisms.during the course of the purification of novel stereospecific secondary aklylsulphohydrolases present in certain detergent-degrading micro-organisms, it became apparent that substrates prepared by sulphating secondary alcohols with h2so4 are heterogeneous. apart from the racemization that occurs if resolved alcohols are sulphated, evidence is provided to show that other isomers are produced in which the position of the ester sulphate group on the alkyl chain has been altered. these changes can b ...1977603632
[effects of the nitrogen nutrition conditions on the growth and protein synthesis of carboxydobacteria].the rate of growth of bacterial strains oxidizing carbon monoxide (pseudomonas gazotropha z-1156, comamonas compransoris z-1155, and seliberia carboxydohydrogena z--1062) was studied as a function of the concentration of nh4cl in the medium. the bacteria could grow on media containing various nitrogen sources (nh4cl, kno3, co(nh2)2). changes in the amino acid content and biochemical composition of the biomass were studied during growth of the bacteria on these media. the biological value of prot ...1979423812
observations on the biological roles of sulphatases.until recently little was known about the biological roles played by sulphatase enzymes, owing in part to the selection of assay substrates that were convenient but only removely related to the natural substrates. once this was recognized the elucidation of function proceeded more rapidly. microbial sulphatases appear to have roles to play in the nutrition of individual microorganisms whilst collectively they enable sulphur, returned to soils and waters in the form of sulphate esters, to be made ...1979398761
purification and properties of the s1 secondary alkylsulphohydrolase of the detergent-degrading micro-organism, pseudomonas c12b.the s1 secondary alkylsulphohydrolase of the detergent-degrading micro-organism, pseudomonas c12b, was separated from other alkylsulphohydrolases and purified to homogeneity. under the experimental conditions used the enzyme completely hydrolysed d-octan-2-yl sulphate (d-1-methylheptyl sulphate), but showed no activity towards the corresponding l-isomer. additional evidence has been obtained to indicate that it is probably optically stereospecific for d-secondary alkyl sulphate esters with the e ...1978206259
[seasonal dynamics of the abundance of vibrios and related microorganisms in the don river].the density of bacteria of the vibrio, aeromonas and comamonas genera the don river was subject to seasonal variations. the maximal vibrio concentration was recorded at the water temperature of 22 degrees c, and of aeromonas and comamonas--at lower temperature. unlike aeromonas, vibrios displayed a reverse dependence of the density on the extent of water contamination.1977146383
vibrio cholerae flagellar antigens: a serodiagnostic test, functional implications of h-reactivity and taxonomic importance of cross-reactions within the vibrio genus.serodiagnostic tests for all serotypes of vibrio cholerae using h-antisera were investigated. activity motile cell lines of 155 stock and international reference cultures of human, animal, fish, and halophilic vibrios, aeromonas, comomonas, pseudomonas, salmonella, and escherichia were investigated. without exception, all cholera vibrios (including the nag serotypes) reacted with h sera. positive reactions were obtained specifically (a) within 2 hrs at 52 degrees c in the tube test using thick f ...197555952
endocarditis associated with comamonas acidovorans.a case of endocarditis caused by comamonas acidovorans (pseudomonas acidovorans) in a 42-year-old intravenous-drug abuser is described. this article appears to be the first detailed report of the isolation of this organism from a systemic clinical infection and its identification as a pathogen.19902298872
heterotrophic plate counts and the isolation of bacteria from mineral waters on selective and enrichment media.the heterotrophic plate counts of 15 brands of bottled non-carbonated mineral waters were examined and found to be generally high and variable. four selective or enrichment media for the enumeration of coliforms (m-endo les and m-lauryl sulphate agar) and pseudomonas aeruginosa (cetrimide-nalidixic acid agar and malachite green broth) were used to isolate several species of gram-negative bacteria. strains identified as cdc gr ivc-2 and comamonas (ps.) acidovorans were the two most commonly isola ...19902126789
4-toluene sulfonate methyl-monooxygenase from comamonas testosteroni t-2: purification and some properties of the oxygenase component.comamonas testosteroni t-2 synthesizes an inducible enzyme system that oxygenates 4-toluene sulfontate (ts) to 4-sulfobenzyl alcohol when grown in ts-salts medium. we purified this ts methyl-monooxygenase system (tsmos) and found it to consist of two components. a monomeric, iron-sulfur flavoprotein (component b), which has been shown to act as a reductase in the 4-sulfobenzoate dioxygenase system of this organism (h. h. locher, t. leisinger, and a. m. cook, biochem. j. 274:833-842, 1991), carri ...19912050632
4-sulphobenzoate 3,4-dioxygenase. purification and properties of a desulphonative two-component enzyme system from comamonas testosteroni t-2.cell-free extracts of comamonas testosteroni t-2 grown in toluene-p-sulphonate/salts medium catalyse the conversion of p-sulphobenzoate (psb) into protocatechuate and sulphite by an nadh-requiring and fe2(+)-activated dioxygenase. anion-exchange chromatography of extracts yielded red (a) and yellow (b) protein fractions, both of which were necessary for dioxygenative activity. further purification of each fraction by hydrophobic interaction chromatography and gel filtration led to two homogeneou ...19912012609
electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groel-type proteins and an attempt to settle the question of whether the multicatalytic proteinase or proteasome exist in all three kingdoms of life--eukaryotes, archaebacteria, and eubacteria--we have undertaken a search for them in the eubacterium comamonas acidovorans. we have, in fact, isolated and purified a cylinder-shaped particle. however, according to various structural and biochemical criteria this turned out to be more reminiscent of the groel protein from escherichia coli and its homologs than to proteas ...19901979749
degradation of p-toluenesulphonic acid via sidechain oxidation, desulphonation and meta ring cleavage in pseudomonas (comamonas) testosteroni t-2.pseudomonas (comamonas) testosteroni t-2 completely converted p-toluenesulphonic acid (ts) or p-sulphobenzoic acid (psb) to cell material, co2 and sulphate, with growth yields of about 5 g protein (mol c)-1. psb and sulphite were excreted as transient intermediates during growth in ts-salts medium. all reactions of a catabolic pathway involving sidechain oxidation and cleavage of the sulphonate moiety as sulphite were measurable in the soluble portion of cell extracts. degradation of ts and psb ...19892614395
identification of d-erythro-dihydroneopterin triphosphate, the first product of pteridine biosynthesis in comamonas sp. (atcc 11299a). 19744213160
central venous catheter-related infection due to comamonas acidovorans in a child with non-hodgkin's lymphoma. 19947811890
transformation of microorganisms with the plasmid vector with the replicon from pac1 from acetobacter pasteurianus.a number of gram-negative and gram-positive bacteria species was screened for the expression of the gram-negative plasmid pack5 and pact72 with replicon of pac1 plasmid from acetobacter pasteurianus. as was described previously, both plasmids were expressed in escherichia coli, acetobacter pasteurianus, acetobacter aceti, shigella spp. and citrobacter spp. expressions of plasmids were successful in twelve species tested, comamonas terrigena, salmonella typhimurium, serratia marcescens, bacillus ...19957832808
regulation of the hema gene during 5-aminolevulinic acid formation in pseudomonas aeruginosa.the general tetrapyrrole precursor 5-aminolevulinic acid is formed in bacteria via two different biosynthetic pathways. members of the alpha group of the proteobacteria use 5-aminolevulinic acid synthase for the condensation of succinyl-coenzyme a and glycine, while other bacteria utilize a two-step pathway from aminoacylated trna(glu). the trna-dependent pathway, involving the enzymes glutamyl-trna reductase (encoded by hema) and glutamate-1-semialdehyde-2,1-aminomutase (encoded by heml), was d ...19957883699
bacteremia with and without meningitis due to yersinia enterocolitica, edwardsiella tarda, comamonas terrigena, and pseudomonas maltophilia. 19705278130
the dna base composition of a flagellar mutant of comamonas terrigena atcc 8461. 19665296608
terephthalate 1,2-dioxygenase system from comamonas testosteroni t-2: purification and some properties of the oxygenase component.comamonas testosteroni t-2, grown in terephthalate (ter)-salts medium, synthesizes inducible enzymes that convert ter to (1r,2s)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylic acid (dcd) and protocatechuate (pc). anion-exchange chromatography of cell extracts yielded two sets of fractions, r and z, that were necessary for oxygenation of ter to dcd; we termed this activity the ter dioxygenase system (terdos). an nad(+)-dependent dcd dehydrogenase, which converted dcd to pc, overlapped all fraction ...19947961417
phenylalanine uptake and phenylalanine-binding material in comamonas sp. (atcc 11299a). 19705439303
partial purification and properties of guanosine triphosphate cyclohydrolase, the first enzyme in pteridine biosynthesis, from comamonas sp. (atcc 11299a). 19715543698
[comparison of antibacterial activity of cefmenoxime with other cephalosporins against clinically isolated bacteria (author's transl)].we examined the antibacterial activity of mx in comparison with those of other ceps, using aerobic gram-positive cocci, aerobic gram-negative bacilli and anaerobic bacteria, 870 strains in total, all isolated from clinical specimens, in 1979 and 1980. against streptococcus, cmx showed superior antibacterial activity than those of cfx, cmz, cxm and ctm. against h. influenzae, cmx also showed superior antibacterial activity than those of cfx, cmx, cxm, ctm and cez. abpc-and pipc-resistant strains ...19816278174
in situ identification of bacterial species in marine microfouling films by using an immunofluorescence immunofluorescence technique was developed for the in situ identification of specific bacteria in marine microfouling films. microorganisms adherent to glass plates after 30 days of immersion in a synthetic seawater system were cultured and classified by biochemical tests, flagellar arrangement, and the api 20e system. all isolates were gram-negative aerobic or facultative motile rods, predominantly pseudomonas spp. rabbit antisera to the five dominant organisms including achromobacter spp., ...19846393875
preliminary observations on alcohol dehydrogenases in comamonas terrigena that exhibit stereospecificity towards secondary alcohols.extracts of the cells of comamonas terrigena, grown under a variety of different conditions, contain two distinct, constitutive, nad-dependent alcohol dehydrogenase enzymes that can be separated by polyacrylamide-gel electrophoresis. one of the enzymes exhibits activity towards d-alkan-2-ols and primary alcohols and the other is active towards l-alkan-2-ols, symmetrical secondary alcohols and probably other positional isomers of secondary alcohols of the l-configuration. methods for the individu ...19806765257
[metabolism of organic nitrogen compounds by carboxydobacteria].the capability to hydrolyze and assimilate creatinine, uric and hippuric acids as sources of nitrogen, carbon and energy was studied in comamonas compransoris z-1155, seliberia carboxydohydrogena z-1062 and pseudomonas gazotropha z-1156. the organisms effectively hydrolyzed these compounds and assimilated their nitrogen under the conditions of autotrophic growth. the carboxydobacteria were also capable of growing in media containing creatinine, uric and hippuric acids and assimilated these compo ...19817219217
[lipid composition of carboxydobacteria].the composition of lipids was studied in three strains of carboxydobacteria, viz. seliberia carboxydohydrogena z-1062, pseudomonas gazotropha z-1156 and comamonas compransoris z-1155. the content of lipids extracted with an alcohol--chloroform mixture varied in these strains from 9 to 13.2%. the main classes were phospholipids (from 62.6 to 77.2% of the overall lipid content). the major phospholipids were phosphatidyl ethanolamine and phosphatidyl choline. moreover, the strains z-1062 and z-1156 ...19807442569
substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates).the substrate specificities of extracellular lipases purified from bacillus subtilis, pseudomonas aeruginosa, pseudomonas alcaligenes, pseudomonas fluorescens, and burkholderia cepacia (former pseudomonas cepacia) and of extracellular polyhydroxyalkanoate (pha) depolymerases purified from comamonas sp., pseudomonas lemoignei, and p. fluorescens gk13, as well as that of an esterase purified from p. fluorescens gk 13, to various polyesters and to lipase substrates were analyzed. all lipases and th ...19957487042
use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates.a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ...19937504429
biosynthesis and characterization of hydroxybutyrate-hydroxycaproate copolymers.most polyhydroxyalkanoates (phas) reported to date fall into one of two broad classes: either hydroxybutyrate-hydroxyvalerate copolymers (typified by the pha produced by alcaligenes eutrophus), or hydroxyoctanoate-rich heteropolymers (typified by the pha produced by pseudomonas oleovorans). few reports of copolymers rich in hydroxybutyrate (hb), but containing a minor proportion of a co-monomer with a higher carbon number than valerate, have appeared. here we report on the biosynthesis and chara ...19957547720
quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from comamonas testosteroni 63. the first two enzymes in quinoline and 3-methylquinoline degradation.the enzymes catalysing the first two steps of quinoline and 3-methylquinoline degradation by comamonas testosteroni 63 were investigated. quinoline 2-oxidoreductase, which catalyses the hydroxylation of (3-methyl-)quinoline to (3-methyl-)2-oxo-1,2-dihydroquinoline, was purified to apparent homogeneity. the native enzyme, with a molecular mass of 360 kda, is composed of three non-identical subunits (87, 32, and 22 kda), occurring in a ratio of 1.16:1:0.83. containing fad, molybdenum, iron, and ac ...19957556204
microbial metabolism of quinoline and related compounds. xvii. degradation of 3-methylquinoline by comamonas testosteroni 63.a bacterial strain which utilizes 3-methylquinoline as sole source of carbon, nitrogen and energy was isolated from activated sludge. on the basis of its morphological and physiological characteristics, this isolate was classified as comamonas testosteroni. four metabolites of 3-methylquinoline degradation were isolated from the culture supernatant and identified as 3-methyl-2-oxo-1,2-dihydroquinoline, 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline, 5,6-dihydroxy-3-methyl-2-oxo-1,2-dihydroquinoli ...19938489738
insights into the catalytic mechanism and active-site environment of comamonas testosteroni delta 5-3-ketosteroid isomerase as revealed by site-directed mutagenesis of the catalytic base 5-3-ketosteroid isomerase (ksi) of comamonas testosteroni catalyzes the isomerization of a wide variety of delta 5(6) and delta 5(10) steroids through the formation of an enzyme bound dienol(ate) intermediate. asp-38 has been strongly implicated in catalysis, apparently serving as a proton shuttle. in this paper the results of a detailed kinetic characterization of the ksi mutants d38e and d38h are presented. both mutants retain significant activity, with kcat and kcat/km values 10(3)-10(4 ...19957578024
purification and characterization of the comamonas testosteroni b-356 biphenyl dioxygenase this report, we describe some of the characteristics of the comamonas testosteroni b-356 biphenyl (bph)-chlorobiphenyl dioxygenase system, which includes the terminal oxygenase, an iron-sulfur protein (ispbph) made up of an alpha subunit (51 kda) and a beta subunit (22 kda) encoded by bpha and bphe, respectively; a ferredoxin (ferbph; 12 kda) encoded by bphf; and a ferredoxin reductase (redbph; 43 kda) encoded by bphg. ispbph subunits were purified from b-356 cells grown on bph. since highly ...19957592440
molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from comamonas testosteroni gz39.three strains of comamonas testosteroni were isolated from river sediment for the ability to degrade phenanthrene; two of the strains also grew on naphthalene, and one strain also grew on anthracene. the homology of the genes for polycyclic aromatic hydrocarbon degradation in these strains to the classical genes (nah) for naphthalene degradation from pseudomonas putida ncib 9816-4 was determined. the three c. testosteroni strains showed no homology to the nah gene probe even under low-stringency ...19968572701
characterization of the interaction between pqq and heme c in the quinohemoprotein ethanol dehydrogenase from comamonas testosteroni.quinohemoprotein ethanol dehydrogenase from comamonas testosteroni (qh-edh) contains two cofactors, 2,7,9-tricarboxy-1h-pyrrolo[2,3-f]quinoline-4,5-dione (pqq) and heme c. since previous studies on the kinetics of this enzyme suggested that both participate in electron transfer, spectroscopic investigations were performed of the oxidized and reduced holo- and apoenzyme (without pqq but with heme c) to reveal the nature of the interaction between the two redox centers. from this it appears that t ...19957626615
characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ...19957641143
biodegradation of polychlorinated biphenyls by rhizobia: a novel finding.metabolism of simple aromatic compounds in rhizobial strains has been a subject of study for a few decades, due either to the significance of nutritional diversity in the inoculum survival during agricultural applications or to the importance of plant phenolics in the microbe-plant cross-talk and signal-transduction. here, we report the capability of rhizobial strains to catabolize polychlorinated biphenyls (pcbs). in order to identify the genes in these strains that mediate the catabolism of pc ...19968579613
aliphatic nitrilase from a soil-isolated comamonas testosteroni sp.: gene cloning and overexpression, purification and primary aliphatic nitrilase, active on adiponitrile and cyanovaleric acid, was identified and purified from comamonas testosteroni sp. (ct). oligodeoxyribonucleotide probes were designed from limited amino acid (aa) sequence information and used to clone the corresponding gene, named nita. high homologies were found at the aa level between ct nitrilase and the sequences of known nitrilases. multi-alignment of sequenced nitrilases suggests that cys163 of ct plays an essential role in the active site. ...19957642130
identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from pseudomonas putida biotype order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from pseudomonas putida biotype b during catalysis, we replaced aspartic acid 40 with asparagine (d40n) and tyrosine 16 with phenylalanine (y16f) in the enzyme by site-directed mutagenesis. both purified mutant enzymes resulted in profound decreases in catalytic activities, 10(3.3)-fold in the y16f mutant and 10(6.2)-fold in the d40n mutant. aspartic acid 40 and tyrosine 16 of the enzyme are the c ...19957730300
sequence of the bphd gene encoding 2-hydroxy-6-oxo-(phenyl/chlorophenyl)hexa-2,4-dienoic acid (hop/cpda) hydrolase involved in the biphenyl/polychlorinated biphenyl degradation pathway in comamonas testosteroni: evidence suggesting involvement of ser112 in catalytic activity.the nucleotide sequence of bphd, encoding 2-hydroxy-6-oxo-(phenyl/chlorophenyl)hexa-2,4-dienoic acid hydrolase involved in the biphenyl/polychlorinated biphenyl degradation pathway of comamonas testosteroni strain b-356, was determined. comparison of the deduced amino-acid sequence with published sequences led to the identification of a 'lipase box', containing a consensus pentapeptide sequence glyxaaserxaagly. this suggested that the mechanism of action of this enzyme may involve an asp-ser-his ...19957737519
isolation and characterization of a bacterium which utilizes polyester polyurethane as a sole carbon and nitrogen source.various soil samples were screened for the presence of microorganisms which have the ability to degrade polyurethane compounds. two strains with good polyurethane degrading activity were isolated. the more active strain was tentatively identified as comamonas acidovorans. this strain could utilize polyester-type polyurethanes but not the polyether-type polyurethanes as sole carbon and nitrogen sources. adipic acid and diethylene glycol were probably the main degradation products when polyurethan ...19957781989
a pseudo-epidemic involving bone allografts.preimplantation cultures of four sterile bone allograft specimens grew comomonas acidovorans and pseudomonas species. an epidemiological investigation, including molecular subtyping methods, revealed that the allograft specimens were contaminated in a microbiology laboratory sonicator water bath.19947890923
description of the kinetic mechanism and the enantioselectivity of quinohaemoprotein ethanol dehydrogenase from comamonas testosteroni in the oxidation of alcohols and aldehydes.initial rate studies were performed on the oxidation of (racemic) alcohols as well as aldehydes by quinohaemoprotein ethanol dehydrogenase, type 1, from comamonas testosteroni with potassium ferricyanide as electron acceptor. the data could be fitted with an equation derived for a mechanism (hexa-uni ping-pong) in which alcohols are oxidized to the corresponding carboxylic acids and the intermediate aldehyde becomes released from the enzyme. however, for some substrates it was necessary to assum ...19948001568
microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in comamonas acidovorans.comamonas acidovorans ds-17 was isolated from activated sludge and found to produce copolymers of 3-hydroxybutyrate (3hb) and 4-hydroxybutyrate (4hb) at 30 degrees c under growth-limited conditions. when 1,4-butanediol or 4-hydroxybutyric acid was used as the sole carbon source, a p(4hb) homopolymer was produced. random copolymers of 3hb and 4hb units were produced on the addition of glucose or 3-hydroxybutyric acid to the culture solution of 4-hydroxybutyric acid. the physical properties of p(3 ...19948011595
regulation of chloro- and methylphenol degradation in comamonas testosteroni jh5.comamonas testosteroni jh5 was isolated from a mixed bacterial culture enriched on different chloro- and methylphenols. the strain completely mineralized a mixture consisting of 4-chlorophenol (4-cp) and 4-methylphenol (4-mp). during degradation of the mixture, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 4-chlorocatechol were detected as short-lived intermediates. mineralization of 4-cp and that of 4-mp occurred successively and were accompanied by diauxic growth, ...19948074514
construction of a dna probe to detect isoquinoline-degrading facilitate the cloning of dna encoding isoquinoline degradation an assay was developed that allowed the rapid visual scoring of the isoquinoline degradation phenotype of single colonies. transposon mutagenesis of one of the isolates. comamonas acidovorans iq3, was performed using tn5, and nine isq-mutants deficient in the ability to utilise isoquinoline as the sole nitrogen source were isolated. these mutants were also incapable of utilising the first metabolite of the isoquinoline degradatio ...19948076251
evidence for multiple adaptive peaks from populations of bacteria evolving in a structured habitat.natural selection tends to promote the divergence of populations living in different environments. even in identical environments, however, replicate populations may diverge if they find alternative adaptive solutions. we describe the evolution of 18 bacterial populations (comamonas sp.) founded from a single progenitor genotype and propagated separately for 1000 generations in two distinct environments, one physically unstructured (mass-action liquid) and the other structured (agar surfaces). p ...19948090765
kinetics and mechanism of heterogeneous hydrolysis of poly[(r)-3-hydroxybutyrate] film by pha depolymerases.the kinetics and mechanism of enzymatic degradation on the surface of poly[(r)-3-hydroxybutyrate] (p[(r)-3hb]) film have been studied using three types of extracellular poly(hydroxyalkanoate) (pha) depolymerases from alcaligenes faecalis, pseudomonas picketti and comamonas testosteroni. the monomer and dimer of 3-hydroxybutyric acid were produced during the course of the enzymatic degradation of p[(r)-3hb] film, and the rate of production was determined by monitoring the increase in absorbance a ...19938110658
extent of proton transfer in the transition states of the reaction catalyzed by the delta 5-3-ketosteroid isomerase of comamonas (pseudomonas) testosteroni: site-specific replacement of the active site base, aspartate 38, by the weaker base alanine-3-sulfinate.previous studies of the mechanism of the steroid isomerase of comamonas (pseudomonas) testosteroni have identified aspartate 38 as the proton porter which transfers the substrate's 4 beta proton to the 6 beta position of the product. consequently, aspartate 38 functions as a base in the deprotonation of the substrate to form a dienol or dienolate intermediate, which then undergoes reprotonation from protonated aspartate 38 at c-6 beta to give the product. we have tried to characterize the transi ...19948117731
mechanism of the reaction catalyzed by delta 5-3-ketosteroid isomerase of comamonas (pseudomonas) testosteroni: kinetic properties of a modified enzyme in which tyrosine 14 is replaced by 3-fluorotyrosine.tyrosine 14 of delta 5-3-ketosteroid isomerase plays an important role in the function of the enzyme, since its replacement by phenylalanine results in a decrease in kcat by a factor of 10(-4.7). this result and the fact that this residue resides in the enzyme's substrate binding site and is in close proximity to c-2 of the bound steroid suggests that it functions as an electrophile in the catalytic mechanism by protonation of or hydrogen bonding to the c-3 carbonyl oxygen of the substrate. in o ...19948117732
telluria mixta (pseudomonas mixta bowman, sly, and hayward 1988) gen. nov., comb. nov., and telluria chitinolytica sp. nov., soil-dwelling organisms which actively degrade polysaccharides.pseudomonas mixta (type strain, acm 1762 [= atcc 49108], an actively dextranolytic species that possesses both lateral and polar flagella, was compared with the strictly aerobic, rod-shaped, chitinolytic bacterium "pseudomonas chitinolytica" acm 3522t (= cncm i-804) (t = type strain), which has a similar flagellation pattern, by performing phenotypic characterization and dna-dna hybridization studies and by analyzing dna base compositions and 16s rrna sequences. our results indicated that "p. ch ...19938427803
uptake of 4-toluene sulfonate by comamonas testosteroni t-2.the mechanism of transport of the xenobiotic 4-toluene sulfonate (ts) in comamonas testosteroni t-2 was investigated. rapid uptake of ts was observed only in cells grown with ts or 4-methylbenzoate as a carbon and energy source. initial uptake rates under aerobic conditions showed substrate saturation kinetics, with an apparent affinity constant (kt) of 88 microm and a maximal velocity (vmax) of 26.5 nmol/min/mg of protein. uptake of ts was inhibited completely by uncouplers and only marginally ...19938432701
characterization of monoclonal antibodies against erwinia carotovora subsp. atroseptica serogroup i: specificity and epitope analysis.the characteristics of two monoclonal antibodies (mabs), a23/1221.59.44.d.3 (1221) and a23/1239.36.64.e.2 (1239), against erwinia carotovora subsp. atroseptica serogroup i produced in this study were compared with those of two other independently obtained mabs, 4g4 in spain and 4f6 in canada, using different strains as immunogen and different screening procedures. the reaction pattern of mabs 1221 and 1239 determined by indirect elisa on over 200 bacterial strains including five e.c. atroseptica ...19957538107
n-carbamoyl-d-amino acid amidohydrolase from comamonas sp. e222c purification and characterization.n-carbamoyl-d-amino acid amidohydrolase was purified 119-fold, with 36% overall recovery from a cell-free extract of comamonas sp. e222c. the purified enzyme was homogeneous as judged by sds/page. the relative molecular mass of the native enzyme was 120,000 and that of the subunit was 40,000. the purified enzyme hydrolyzed various n-carbamoyl-d-amino acids to d-amino acids, ammonia and carbon dioxide. n-carbamoyl-d-amino acids having hydrophobic groups served as good substrates for the enzyme. t ...19938462543
vascular catheter-related comamonas acidovorans bacteremia managed with preservation of the catheter. 19968895931
carbonyl reduction by 3 alpha-hsd from comamonas testosteroni--new properties and its relationship to the scad family. 19938493916
rp4::mu3a-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway.chromosomal dna fragments encoding the ability to utilize biphenyl as sole carbon source (bph+) were mobilized by means of plasmid rp4::mu3a from strain jb1 (tentatively identified as burkholderia sp.) to alcaligenes eutrophus ch34 at a frequency of 10(-3) per transferred plasmid. the mobilized dna integrated into the recipient chromosome or was recovered as catabolic prime plasmids. three bph+ prime plasmids were transferred from a. eutrophus to escherichia coli and back to a. eutrophus without ...19968969525
molecular analysis of isophthalate and terephthalate degradation by comamonas testosteroni yzw-d.comamonas testosteroni yzw-d was isolated from passaic river sediment for its ability to degrade isophthalate and terephthalate. degradation of the two isomeric compounds proceeds via separately inducible catabolic pathways that converge at protocatechuate. analysis of the catabolic pathways by which these two isomers are degraded demonstrated that a cis-dihydrodiol intermediate is involved in both pathways. the genes for the conversion of isophthalate and terephthalate to protocatechuate were c ...19958565920
quinohaemoprotein ethanol dehydrogenase from comamonas testosteroni. purification, characterization, and reconstitution of the apoenzyme with pyrroloquinoline quinone analogues.pyrroloquinoline-quinone(pqq)-free quinohaemoprotein ethanol dehydrogenase (qh-edh) apoenzyme was isolated from ethanol-grown comamonas testosteroni. the purified apoenzyme, showing a single band of 71 kda on native gel electrophoresis, could be only partially converted into active holoenzyme by addition of pqq in the presence of calcium ions. in addition to a band with a molecular mass of 71 kda, additional bands of 51 kda and 25 kda were observed with sds/page. analysis of the n-terminal seque ...19957601151
characterization of the extracellular poly(3-hydroxybutyrate) depolymerase of comamonas sp. and of its structural gene.the poly(3-hydroxybutyrate) (phb) depolymerase structural gene of comamonas sp. (phazcsp) was cloned in escherichia coli and identified by halo formation on phb-containing solid medium. the nucleotide sequence of a 1719 base pair mboi fragment was determined and contained one large open reading frame (orf1, 1542 base pairs). this open reading frame encoded the precursor of the phb depolymerase (514 amino acids; mr, 53,095), and the deduced amino acid sequence was in agreement with the n-terminal ...19957606660
identification of burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium.we evaluated 819 isolates referred to us as "burkholderia cepacia" from cystic fibrosis (cf) clinics and research laboratories from five countries; 28 (3.4%) were not b. cepacia. a further 12 (1.5%) organisms appeared to be other burkholderia species, but identification could not be confirmed by conventional means. the most prevalently misidentified organisms were stenotrophomonas maltophilia, alcaligenes xylosoxidans, and comamonas acidovorans. many of these organisms grew on oxidation-fermenta ...19979041399
sutterella wadsworthensis gen. nov., sp. nov., bile-resistant microaerophilic campylobacter gracilis-like clinical isolates.campylobacter gracilis (formerly bacteroides gracilis) is an asaccharolytic, nitrate-positive, urease-negative organism that requires formate and fumarate or hydrogen as a growth additive and may pit agar media. clinical isolates that were obtained primarily from appendiceal and peritoneal fluid specimens and initially were identified in our laboratory as b. gracilis were later found to include "unusual" strains that could be distinguished by biochemical and genetic criteria. these unusual c. gr ...19968573504
occurrence of polyhydroxyalkanoic acid granule-associated proteins related to the alcaligenes eutrophus h16 ga24 protein in other bacteria.fifty different polyhydroxyalkanoic acid (pha)-accumulating bacterial strains were investigated for the occurrence of phasin proteins bound to pha granules and related to the ga24 protein of alcaligenes eutrophus h16, by isolating pha granules and western blot analysis of granule-associated proteins employing antibodies raised against the ga24 protein. it could be demonstrated that th pha granules of many poly(3-hydroxybutyrate)-accumulating bacteria exhibited a similar protein pattern, and a pr ...19968598273
purification and characterization of a prokaryotic xanthine dehydrogenase from comamonas acidovorans.xanthine dehydrogenase (xdh) is induced in comamonas acidovorans cells incubated in a limited medium with hypoxanthine as the only carbon and nitrogen source. the enzyme has been purified to homogeneity using standard techniques and characterized. it contains two subunits with m(r) values of 90 and 60 kda. gel filtration studies show the enzyme to have an alpha 2 beta 2 native structure. no precursor form of the enzyme is observed on western blot analysis of cell extracts obtained at various sta ...19968611534
crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from comamonas testosteroni attc 11996.the enzyme 3 (or 17) beta-hydroxysteroid dehydrogenase from comamonas testosteroni was crystallized. crystals, of up to 0.6 mm in their longest dimension and suitable for a crystallographic analysis have been obtained by the vapour diffusion method. they belong to the orthorhombic lattice type and diffract to a maximum resolution of 0.23 nm. a final data set obtained by merging data from three crystals resulted in a completeness of 90% with an rmerge of 6%. a molecular replacement search carried ...19968617258
broviac catheter-related bacteraemias due to unusual pathogens in children with cancer: case reports with literature review.among 102 episodes of intravenous catheter related bacteraemias documented between january 1989 and july 1996 in children receiving antineoplastic chemotherapy or bone marrow transplantation at g. gaslini children's hospital, genoa, italy, were identified seven episodes due to unusual pathogens: bacillus circulans, bacillus licheniformis, brevibacterium casei, flavimonas oryzihabitans, porphyromonas asaccharolytica, comamonas acidovorans and agrobacterium radiobacter. susceptibility to different ...19979200028
characterization of active recombinant his-tagged oxygenase component of comamonas testosteroni b-356 biphenyl dioxygenase.biphenyl (bph) dioxygenase oxidizes bph to 2,3-dihydro-2,3-dihydroxybiphenyl in comamonas testosteroni b-356. the enzyme comprises a two-subunit iron-sulfur protein (ispbph), a ferredoxin ferbph, and a ferredoxin reductase redbph. redbph and ferbph transfer electrons from nadh to an fe-s active center of ispbph which activates molecular oxygen for insertion into the substrate. in this work b-356 ispbph complex and its alpha and beta subunits were purified from recombinant escherichia coli strain ...19968626504
porin from the halophilic species ectothiorhodospira vacuolata: cloning, structure of the gene and comparison with other porins.the gene coding for the anion-specific porin of the halophilic eubacterium ectothiorhodospira (ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. it encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. comparison with aa sequences of porins from several other members of the proteobacteria revealed little homology. only two regions showed local homology with the previously sequen ...19979218724
isolation and characterization of resin acid degrading bacteria found in effluent from a bleached kraft pulp mill.thirteen resin acid degrading bacteria enriched on abietic or dehydroabietic acids were isolated from waste water from the aerated stabilization basin of a bleached kraft pulp mill. standard biochemical tests were used to characterize each isolate. each isolate was tested for its ability to degrade six abietane- and pimarane-type resin acids. resin acid concentrations were determined by high pressure liquid chromatography and uv absorbance. cluster analysis based on phenotypic characteristics id ...19968640603
chemical structure of lipid a isolated from comamonas testosteroni lipopolysaccharide.the chemical structure of lipid a of lipopolysaccharide isolated from comamonas testosteroni was determined by quantitative analysis, methylation analysis, mass spectrometry and nmr spectroscopy. the lipid a backbone was found to consist of 6-o-(2-deoxy-2-amino-beta-d-glucopyranosyl)-2-deoxy-2-amino-alpha-d-g luc ose which was phosphorylated in positions 1 and 4'. hydroxyl groups at positions 4 and 6' were unsubstituted, and position 6' of the non-reducing terminal residue was identified as the ...19968647087
identification and mapping of the gene translation products involved in the first steps of the comamonas testosteroni b-356 biphenyl/chlorobiphenyl biodegradation this study, we have mapped comamonas testosteroni b-356 genes encoding enzymes for the conversion of biphenyl and 4-chlorobiphenyl into the corresponding meta-cleavage compounds onto a 6.3-kb dna fragment, and we have determined the subunit composition of the enzymes involved in this pathway. the various proteins encoded by this 6.3-kb dna fragment and by subclones derived from it were overexpressed and selectively labelled using the t7 polymerase promoter system in escherichia coli. they wer ...19947954108
characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of comamonas testosteroni.the gene encoding quinohaemoprotein ethanol dehydrogenase type i (qh-edhi) from comamonas testosteroni has been cloned and sequenced. comparison of the amino acid sequence deduced from this with that determined for the n-terminal amino acid stretch of purified qh-edhi, suggests that the gene also contains a leader sequence of 31 residues. based on this information, the molecular mass of the apo-enzyme, i.e. the enzyme without the cofactors pyrroloquinoline quinone (pqq) and haem c, and without t ...19968654419
comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.comamonas testosteroni delta 4(5 alpha)- and delta1-dehydrogenases [delta4(5alpha)- and delta1dh] are key enzymes in the degradation of steroids having an a:b ring fusion in a trans configuration. we previously reported the isolation of the delta1dh gene (p. plesiat, m. grandguillot, s. harayama, s. vragar, and y. michel briand, j. bacteriol. 173:7219-7227, 1991). in this study, the gene encoding delta 4(5 alpha)dh was cloned in escherichia coli on a 16-kbp bamhi fragment by screening a genomic ...19968655514
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
comamonas testosteroni colony phenotype influences exopolysaccharide production and coaggregation with yeast cells.a comamonas testosteroni strain was isolated from activated sludge on the basis of its ability to coaggregate with yeast cells. on agar plates the following two types of colonies were formed: colonies with a mucoid appearance and colonies with a nonmucoid appearance. on plates this strain alternated between the two forms, making sectored colonies. in liquid medium with constant agitation no such change was observed. in the absence of agitation and in contact with a glass surface a culture with p ...19968702260
characterization of active recombinant 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from comamonas testosteroni b-356 and sequence of the encoding gene (bphb).2,3-dihydro-2,3-dihydroxybiphenyl-2,3-dehydrogenase (b2,3d) catalyzes the second step in the biphenyl degradation pathway. the nucleotide sequence of comamonas testosteroni b-356 bphb, which encodes b2,3d, was determined. structural analysis showed that the dehydrogenases involved in the bacterial degradation of aromatic compounds are related to each other and that their phylogenetic relationships are very similar to the relationships observed for dioxygenases that catalyze the initial reaction ...19968702262
the structure and characterization of the surface protein layer of a comamonas-like organism.the regular surface layer of a strain of a comamonas-like organism was examined by electron microscopy. the surface layer protein was easily extracted from the cell surface by a 2.5 m solution of lithium chloride. the protein subunit has a molecular size of 32,000 daltons, but usually forms a large aggregate of more than 1,200,000 daltons. in the extract it formed a regular array of p4 symmetry and was observed to be intimately associated with fragments of lipopolysaccharide. the size of a subun ...19958789053
genetics of naphthalene and phenanthrene degradation by comamonas testosteroni.naphthalene and phenanthrene have long been used as model compounds to investigate the ability of bacteria to degrade polycyclic aromatic hydrocarbons. the catabolic pathways have been determined, several of the enzymes have been purified to homogeneity, and genes have been cloned and sequenced. however, the majority of this work has been performed with fast growing pseudomonas strains related to the archetypal naphthalene-degrading p. putida strains g7 and ncib 9816-4. recently comamonas testos ...19979451837
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