distribution and effect of the weak acids 5,5-dimethyl-2,4-oxazolidinedione (dmo) and 2,4-dinitrophenol (dnp) in membrane vesicles of micrococcus denitrificans. 19769905
multiple low spin forms of the cytochrome c ferrihemochrome. epr spectra of various eukaryotic and prokaryotic cytochromes c.1. despite the same methionine-sulfur:heme-iron:imidazole-nitrogen hemochrome structure observed by x-ray crystallography in four of the seven c-type eukaryotic and prokaryotic cytochromes examined, and the occurrence of the characteristic 695 nm absorption band correlated with the presence of a methionine-sulfur:heme-iron axial ligand in all seven proteins, they fall into two distinct classes on the basis of their epr and optical spectra. the horse, tuna, and bakers' yeast iso-1 cytochromes c ...197713072
metabolic regulation of malic enzyme activity from paracoccus denitrificans by glyoxylate and acetylcoa. 197714633
sulphur metabolism in paracoccus denitrificans. purification, properties and regulation of serine transacetylase, o-acetylserine sulphydrylase and beta-cystathionase.1. serine transacetylase, o-acetylserine sulphydrylase and beta-cystathionase were purified from paracoccus denitrificans strain 8944. 2. serin transacetylase was purified 150-fold. the enzyme has a ph optimum between 7.5 and 8.0, is specific for l-serine and is inhibited by sulphydryl-group reagents. the apparent km values for serine and acetyl-coa are 4.0 - 10(-4) and 1.0 - 10(-4) m, respectively. serine transacetylase is strongly inhibited by cysteine. 3. o-acetylserine sulphydrylase was puri ...197714692
sulphur metabolism in paracoccus denitrificans. purification, properties and regulation of cysteinyl-and methionyl-trna synthetase.cysteinyl- and methionyl-trna synthetases (ec 6.11.-) were purified 1200- and 1000-fold, respectively, from sonic extracts of paracoccus denitrificans strain 8944, and kinetics, substrate specificity and regulatory properties were determined using the atp-ppi exchange reaction. both enzymes had ph optima of approx. 8 and were inhibited by sulphydryl-group reagents. cysteinyl-trna synthetase catalysed l-selenocysteine- and alpha-aminobutyric acid-dependent atp-ppi exchange and methionyl-trna synt ...197714693
metabolic regulation of the glucose-6-phosphate dehydrogenase from paracoccus denitrifcans grown on glucose/nitrate.glucose-6-phosphate dehydrogenase (d-glucose-6-phosphate : nadp+ l-oxidoreductase ec isolated from paracoccus denitrificans grown on glucose/nitrate exhibits both nad+-and nadp+- linked activities. both activities have a ph optimum of ph 9.6 (glycine/naoh buffer) and neither demonstrates a mg2+ requirement. kinetics for both nad(p)+ and glucose-6-phosphate were investigated. phosphoenolpyruvate inhibits both activities in a competitive manner with respect to glucose-6-phosphate. atp in ...197715531
the bioenergetics of paracoccus denitrificans. 197720140
nitrate and nitrite reduction by liquid membrane-encapsulated whole cells. 197521712
transmembrane electrical and ph gradients of paracoccus denitrificans and their relationship to oxidative phosphorylation. 197824551
electron-transport chain and coupled oxidative phosphorylation in methanol-grown paracoccus denitrificans. 197829587
studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase.antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ...197831146
respiration-dependent proton translocation and the transport of nitrate and nitrite in paracoccus denitrificans and other denitrifying bacteria. 197831172
from extracellular to intracellular: the establishment of mitochondria and chloroplasts.paracoccus and rhodopseudomonas are unusual among bacteria in having a majority of the biochemical features of mitochondria; blue-green algae have many of the features of chloroplasts. the theory of serial endosymbiosis proposes that a primitive eukaryote successively took up bacteria and blue-green algae to yield mitochondria and chloroplasts respectively. possible characteristics of transitional forms are indicated both by the primitive amoeba, pelomyxa, which lacks mitochondria but contains a ...197936620
energy transduction in the mitochondrionlike bacterium paracoccus denitrificans during carbon- or sulphate-limited aerobic growth in continuous culture.paracoccus denitrificans was grown in carbon-limited aerobic continuous culture (critical dilution rate (dc) = 0.48 h-1). the molar growth yield for carbon (succinate or malate) was constant at about 60 over a broad dilution range (growth rate) from 0.10 to 0.48 h-1. measurements of the stoichiometry of proton translocation associated with the oxidation of endogenous substrates yielded a ratio of protons ejected from the cell per atom of oxygen consumed(leads to h+:o) of 8.55 which decreased to ...197836970
proton translocation coupled to ubiquinol oxidation in paracoccus denitrificans. 197936972
anaerobic respiration and energy conservation in paracoccus denitrificans. functioning of iron-sulfur centers and the uncoupling effect of nitrite.1. electron paramagnetic resonance spectra at 8-60 k of nadh-reduced membrane particles prepared from paracoccus denitrificans grown anaerobically with nitrate as terminal electron acceptor show the presence of iron-sulfur centers 1-4 in the nadh-ubiquinone segment of the respiratory chain. in addition resonance lines at g = 2.058, g = 1.953 and g = 1.88 are detectable in the spectra of succinate-reduced membranes at 15 k, which are attributed to the iron-sulfur-containing nitrate reductase. 2. ...197937082
comparison of the membrane-bound and detergent-solubilised hydrogenase from paracoccus denitrificans. isolation of the hydrogenase.the hydrogenase from paracoccus denitrificans is an integral membrane protein and has been solubilised by triton x-100. the membrane-bound and detergent-solubilised forms of the enzyme have been compared. both forms of the enzyme show a ph optimum for reduction of benzyl viologen at ph 8.5--9.0 and are both inhibited by concentrations of nacl greater than 30 mm. an arrhenius plot of the activity of hydrogenase in the membrane shows no 'break'. the form of the arrhenius plot and the activation en ...197939613
regulation of respiration in paracoccus denitrificans: the dependence on redox state of cytochrome c and [atp]/[adp][pi]. 197941484
the inhibitor-sensitivity of the plasma-membrane adenosine triphosphatase of paracoccus denitrificans: comparison with the mitochondrial adenosine triphosphatase [proceedings]. 197772696
[inorganic ion transport in bacteria]. 1979107670
the mitochondrial ribosomes of neurospora crassa. ii. comparison of the proteins from neurospora crassa mitochondrial ribosomes with ribosomal proteins from neurospora cytoplasm, from rat liver mitochondria and from bacteria.1. it has been shown by datema et al. (datema, r., agsteribbe, e. and kroon, a.m. (1974) biochim. biophys. acta 335, 386--395) that neurospora mitochondria isolated in a mg2+-containing medium (or after homogenization of the mycelium in this medium and subsequent washing of the mitochondria in edta-containing medium) possess 80-s ribosomes; mitochondria homogenized and isolated in edta medium yield 73-s ribosomes. the ribosomal proteins of the subunits of 80-s and 73-s ribosomes were compared by ...1976133723
possible cross-reactivity of human anti-mitochondrial antibodies with membrane vesicles of paracoccus denitrificans. 1976137140
tightly bound nucleotides of the energy-transducing atpase, and their role in oxidative phosphorylation. i. the paracoccus denitrificans system.1. the coupling atpase of paracoccus denitrificans can be removed from the membrane by washing coupled membrane fragments at low salt concentrations. 2. this atpase resembles coupling atpases of mitochondria, chloroplasts and other bacteria. it is a negatively charged protein of molecular weight about 300,000. an inhibitor protein in bound tightly to the atpase in vivo, and can be destroyed by trypsin treatment. 3. atp and adp are found tightly bound to the coupling atpase of p. denitrificans, b ...1977139162
bacterial respiration. 1977140652
some contemporary problems in electron-transport-linked adenosine triphosphate synthesis and related processes. 1977143382
the interactions of coupling atpases with nucleotides. 1978147104
the cytochrome fold and the evolution of bacterial energy metabolism. 1976176369
amino acid sequence of paracoccus denitrificans cytochrome c550.the amino acid sequence of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been established by a combination of standard chemical techniques and interpretation of a 2.5 a resolution x-ray electron density map. peptides derived from a trypsin digest were chemically sequenced, and then ordered by fitting them to the density map. the amino acid compositions of chymotryptic peptides confirmed the x-ray map ordering the tryptic peptides. the amino acid sequence of this respiratory ...1976177408
oxidative phosphorylation in micrococcus denitrificans: calculation of the p/o ratio in growing cells.p/o ratios were measured in membrane particles obtained from cells of micrococcus denitrificans, while growing on different carbon sources. the membrane particles obtained from cells growing actively on glucose, succinate, ethanol and propanol as the carbon and energy sources catalyzed oxidative phosphorylation and yielded respective p/o ratios of 1.4, 1.2, 0.8, and 0.5 with nadh, and 0.8, 0.6, 0.6, and 0.5 with succinate as the electron donors. not such a difference in p/o ratio is observed in ...1976179486
the structure of paracoccus denitrificans cytochrome c550.the crystal structure of paracoccus (formerly micrococcus) denitrificans cytochrome c550 has been solved by x-ray diffraction to a resolution of 2.45 a. in both amino acid sequence and molecular structure it is evolutionarily homologous with mitochondrial cytochrome c from eukaryotes and photosynthetic cytochrome c2 from purple non-sulfur bacteria. all of these cytochromes c have the same basic folding pattern, with surface insertions of extra amino acids in c550. various strains of c2 have all, ...1976180013
pathway of thiamine pyrophosphate synthesis in micrococcus denitrificans.the pathway of thiamine pyrophosphate (tpp) biosynthesis, which is formed either from exogeneously added thiamine or from the pyrimidine and thiazole moieties of thiamine, in micrococcus denitrificans was investigated. the following indirect evidence shows that thiamine pyrophosphokinase (ec catalyzes the synthesis of tpp from thiamine: (i) [35s]thiamine incubated with cells of this microorganism was detected in the form of [35s]thiamine; (ii) thiamine gave a much faster rate of tpp syn ...1976181359
paracoccus denitrificans: a present-day bacterium resembling the hypothetical free-living ancestor of the mitochondrion. 1975183302
internally generated reduced nicotinamide adenine dinucleotide as a substrate for glycine transport by membrane vesicles of paracoccus denitrificans.internally generated reduced nicotinamide adenine dinucleotide was the most efficient substrate for glycine transport by membrane vesicles of paracoccus denitrificans.1977187573
reduction of iron and synthesis of protoheme by spirillum itersonii and other organisms.membranes from spirillum itersonii reduce ferric iron to ferrous iron with reduced nicotinamide adenine dinucleotide or succinate as a source of reductant. iron reduction was measured spectrophotometrically at 562 nm using ferrozine, which chelates ferrous iron specifically. reduced nicotinamide adenine dinucleotide or succinate was also effective as a source of iron. the effects of respiratory inhibitors suggested that reduction of iron occurs at one or more sites on the respiratory chain befor ...1977190208
specific thiamine monophosphate phosphohydrolase in micrococcus denitrificans.a phosphohydrolase specific for thiamine monophosphate was isolated from micrococcus denitrificans, partially purified approximately 100-fold, and separated from acid phosphatase.1977192716
purification and properties of an acid phosphatase of micrococcus denitrificans distinct from thiamine phosphate determine whether the acid phosphatase in micrococcus denitrificans participates in hydrolysis of thiamine phosphate in the synthesis of thiamine pyrophosphate, acid phosphatase was purified 280-fold by conventional procedures, which removed thiamine phosphate phosphatase completely. studies showed that this acid phosphatase is a different protein from thiamine phosphate phosphatase and that it has no binding site for thiamine phosphate on its active site.1977197075
structure and function of cytochromes c. 1977197877
micromorphology of gram-negative hydrogen bacteria. ii. cell envelope, membranes, and cytoplasmic inclusions.the fine structure of the cell envelope, of membrane systems and of cytoplasmic inclusions of gram-negative aerobic hydrogen bacteria has been studied. the results have been tabulated, and three main groups could be recognized: group 1: alcaligenes eutrophus, a.paradoxus, a.ruhlandii, pseudomonas facilis, p.flava, p.pseudoflava, p.palleronii, and aquaspirillum autotrophicum; group 2: "corynebacterium" autotrophicum and strains ma 2 and sa 35; group 3: paracoccus denitrificans. special structur ...1977199126
the role of iron-sulfur center 2 in electron transport and energy conservation in the nadh-ubiquinone segment of the respiratory chain in paracoccus denitrificans.1. the electron paramagnetic resonance spectra at 15 k of reduced membrane particles of paracoccus denitrificans exhibit resonance signals with g values, line shapes and temperature profile which are similar to the signals of the iron-sulfur centers observed in the nadh-ubiquinone segment of mitochondrial respiratory chains. these iron-sulfur centers are reducible with nadh, nadph as well as chemically with dithionite. 2. sulphate-limited growth of paracoccus denitrificans results in the loss of ...1977202453
regulation of energy metabolism: evidence against a primary role of adenine mucleotide translocase. 1978204515
growth yields and the efficiency of oxidative phosphorylation during autotrophic growth of paracoccus denitrificans on methanol and formate. 1978211973
the protonmotive force in phosphorylating membrane vesicles from paracoccus denitrificans. magnitude, sites of generation and comparison with the phosphorylation potential.1. the magnitude of the protonmotive force in phosphorylating membrane vesicles from paracoccus denitrificans was estimated. the membrane potential component was determined from the uptake of s(14)cn(-), and the transmembrane ph gradient component from the uptake of [(14)c]methylamine. in each case a flow-dialysis technique was used to monitor uptake. 2. with nadh as substrate, the membrane potential was about 145mv and the ph gradient was below 0.5 ph unit. the membrane potential was decreased ...1978212022
the variable cytochrome content of paracoccus denitrificans grown aerobically under different conditions. 1978213309
purification of bacterial cytochrome c by isoelectric focusing. 1978213681
studies of the orientation of the mitochondrial redox carriers. iii. orientation of the gx and gy axes of the hemes of cytochrome oxidase with respect to the plane of the membrane in oriented membrane multilayers.the epr absorption properties of the hemes of cytochrome oxidase and their liganded derivatives were examined in oriented multilayers from isolated oxidase, mitochondrial membranes and membrane fragments of a bacterium, paracoccus denitrificans. the hemes of the oxidase in all the systems investigated were oriented normal to the plane of the multilayers. the directions of the g signals corresponding to the gx and gy axes of the g tensor were found to be different in low-spin ferric heme in fully ...1979216401
on the current-voltage relationships of energy-transducing membranes: phosphorylating membrane vesicles from paracoccus denitrificans [proceedings]. 1978217778
evidence for water as the product for oxygen reduction by cytochrome cd. 1979223563
implications of the integrated rate law for the reactions of paracoccus denitrificans nitrite reductase.the integrated rate law for the reaction of the nitrite reductase of paracoccus denitrificans, a cytochrome cd, has been established for turnover assays using donor ferrocytochromes c and either nitrite or molecular oxygen as the ultimate acceptor. the time course for the concentration of ferrocytochrome follows the law: formula: (see text), where s is the concentration of donor ferrocytochrome c, so is the initial concentration, t is time, and u1, u2, and u3 are empirical parameters that are co ...1979226118
the induction of site 3 phosphorylation in heterotrophically grown paracoccus denitrificans in the chemostat on mannitol plus methanol [proceedings]. 1979227325
structural aspects of the dye-linked alcohol dehydrogenase of rhodopseudomonas acidophila.1. a dye-linked alcohol dehydrogenase was purified 60-fold from extracts of rhodopseudomonas acidophila 10050 grown aerobically on ethanol. 2. the properties of this enzyme were identical with those of the alcohol dehydrogenase synthesized by this organism during growth on methanol anaerobically in the light, and they are judged to be the same enzyme. 3. the enzyme gave a single protein band, coincident with alcohol dehydrogenase activity, during electrophoresis on polyacrylamide gel. 4. the ami ...1979229820
paracoccus denitrificans and the evolutionary origin of the mitochondrion. 1975235742
the identification and biosynthesis of siderochromes formed by micrococcus denitrificans.1. micrococcus denitrificans excretes three catechol-containing compounds, which can bind iron, when grown aerobically and anaerobically in media deficient in iron, and anaerobically in medium with a high concentration of ca2+. 2. one of these compounds was identified as 2,3-dihydroxybenzoic acid (compound i), and the other two were tentatively identified as n1n8-bis-(2,3-dihydroxybenzoyl)spermidine (compound ii) and 2-hydroxybenzoyl-n-l-threonyl-n4[n1n8-bis-(2,3-dihydroxybenzoyl)]spermidine (co ...1975238503
irradiation of micro-organisms with mono-energetic x-rays; biological consequences of the auger effect.the radiation resonance effect reported previously for isolated biomolecules has now for the first time been observed in a cellular system. dried bacteria, micrococcus denitrificans, in which tdr in dna was partially substituted by budr, were subjected to mono-energetic x-rays of energies below or above the k-edge for br. subsequently, the colony-forming ability was assayed. for photon energy slightly above the k-edge, the lethality/rad was greater than that below the k-edge. this is interpreted ...1978309443
aerobic and anaerobic bacterial respiration monitored by electrodes.a technique is described by which both oxygen and nitrate (or nitrate or chlorate) levels were continuously monitored during bacterial respiration. paracoccus (micrococcus) denitrificans and escherichia coli oxidizing succinate rapidly ceased to reduce nitrate when oxygen was available, and equally rapidly commenced nitrate reduction when all the oxygen had been consumed. by contrast, membrane vesicles isolated from p. denitrificans reduced oxygen and nitrate simultaneously. the respiratory nitr ...1977319200
substrate binding site for nitrate reductase of escherichia coli is on the inner aspect of the membrane.escherichia coli grown anaerobically on nitrate exhibited the same transport barrier to reduction of chlorate, relative to nitrate, as that exhibited by paracoccus denitrificans. this establishes that the nitrate binding site of nitrate reductase (ec in e. coli must also lie on the cell side of the nitrate transporter which is associated with the plasma membrane. because nitrate reductase is membrane bound, the nitrate binding site is thus located on the inner aspect of the membrane. n ...1979374343
micromorphology of gram-negative hydrogen bacteria. i. cell morphology and flagellation.the cell morphology, the arrangement and fine structure of flagella and the piliation of the following gram-negative aerobic hydrogen bacteria have been studied: alcaligenes eutrophus, alcaligenes paradoxus, alcaligenes ruhlandii, pseudomonas flava, pseudomonas pseudoflava, pseudomonas palleronii, pseudomonas facilis, aquaspirillum autotrophicum, paracoccus denitrificans, corynebacterium autotrophicum, and strains ma 2 and sa 35. the identity of the bacteria was examined by their substrate spect ...1977410385
induction by cyanide of cytochrome d in the plasma membrane of paracoccus denitrificans. 1979437107
evidence from 31p nuclear magnetic resonance that polyphosphate synthesis is a slip reaction in paracoccus denitrificans [proceedings]. 1979437269
[relationship of hydrogen bacteria to carbon monoxide].the purpose of this work was to study the ability of various cultures of hydrogen bacteria to grow in a gaseous mixture containing, apart from h2, o2 and co2, carbon monoxide at a concentration of 10% (v/v) and higher. in contrast to co-oxidizing bacteria, these organisms could not use co as a sole source of energy. among 17 studied strains of hydrogen bacteria, only those belonging to the group alcaligenes eutrophus could withstand 10% of co (v/v) in a gaseous mixture.1979440170
respiration-driven accumulation of c4 dicarboxylic acids by isolated membrane vesicles of paracoccus denitrificans. 1979455122
hydrodynamic parameters of the detergent-solubilised hydrogenase from paracoccus denitrificans.the hydrogenase from paracoccus denitrificans, which is an intrinsic membrane protein, has been solubilised from membranes by triton x-100. the partial specific volume of the solubilised protein has been determined using sucrose density gradient centrifugation in h2o and 2h2o. the values of the specific volumes of hydrogenase, measured in the presence or absence of triton x-100, are 0.73 and 0.74 ml . g-1, respectively, indicating that hydrogenase binds much less than one micelle of triton x-100 ...1979477660
transfer of kanamycin resistance mediated by plasmid r68.45 in paracoccus denitrificans.plasmid r68.45 mediates the transfer of kanamycin resistance from pseudomonas aeruginosa to paracoccus denitrificans. kanamycin resistance could be transferred from one strain of p. denitrificans to another, thus opening up the possibility of using r68.45 as a sex factor in p. denitrificans.1979479105
[elimination of nitrous oxide by a combined bacterial culture].the ability of bacteria to eliminate nitrous oxide (n2o) from a gaseous phase containing 20% o2 was studied. representatives of the following physiological groups were found to be incapable of removing n2o: denitrifying bacteria (paracoccus denitrificans), nitrifying bacteria (nitrosospira briensis), carboxydobacteria (pseudomonas carboxydoflava), methane oxidizing bacteria (methylosinus trichosporium), and nitrogen fixing bacteria (bacillus polymyxa). five corynebacteria-like strains were isola ...1979481272
interaction of lipophilic quinones with membrane fragments of paracoccus denitrificans and staphylococcus quinone-depleted mitochondrial and paracoccus denitrificans membranes the quantum yield of fluorescence of ostruthin (6-geranyl-7-hydroxycoumarin) was maintained, whereas an increase in the quantum yield took place after extraction of staphylococcus epidermidis membrane. a marked quenching effect of ubiquinone and menaquinone each with two isoprene units in the side chain on the ostruthin fluorescence was found with all types of quinone-depleted particles. when the homogues of menaquinone and ...1979486107
[utilization of monofluorobenzoic acids by paracoccus denitrificans bacteria]. 1979530142
structural analysis of four strains of paracoccus denitrificans.two out of eleven newly isolated strains of paracoccus denitrificans were investigated by light and electron microscopic methods and compared with two strains of p. denitrificans already kept in culture collections. samples were taken from different growth phases revealing short rods and nearly spherical cells in the exponential growth phase, and an increasing ratio of nearly spherical cells in the stationary growth phase. cell division followed the binary fission mode; higher cell aggregates we ...1979582887
anaerobic respiration and energy conservation in paracoccus denitrificans. functioning of iron-sulfur centers, the uncoupling action of nitrite and stoichiometries for proton pumping [proceedings]. 1979582899
rapid oxygen-induced reduction of b-type cytochromes in paracoccus denitrificans. 1978620789
ribulose bisphosphate carboxylase from methanol-grown paracoccus denitrificans.paracoccus denitrificans grows on methanol as the sole source of energy and carbon, which it assimilates aerobically via the reductive pentose phosphate cycle. this gram-negative bacterium grew rapidly on 50 mm methanol (generation time, 7 h, 30 degrees c) in excellent yield (3 g of wet-packed cells per liter of culture). electron microscopic studies indicated that the late-log-phase cells were coccoid, having a thick envelope surrounding a layer of more diffuse electron-dense material and a rel ...1978659365
hydrogenase activity in paracoccus denitrificans. partial purification and interaction with the electron transport chain. 1978667184
the preparation of tightly coupled membrane vesicles from paracoccus denitrificans. 1978677462
dissimilatory nitrate reduction by liquid membrane encapsulated cell-free extracts and whole cells of micrococcus denitrificans.the combination of sephadex g-15 and ion-exchange resin columns allows one-step desalting and separation of cellodextrins using water as the sole eluent. the column apparatus described in this paper has the potential of producing up to 3 g cellodextrins in one day. in addition, the columns described are stable and do not require repacking or regeneration after each run. hence the potential exists for scaling up this system for even greater production of cellodextrins if need be.1978708860
purification of (4fe-4s)1--2--ferredoxins (high-potential iron--sulfur proteins) from bacteria. 1978713842
purification of electron-transfer components of nitrifying and denitrifying bacteria and assay of intermediates. 1978713858
aerobic and anaerobic growth of paracoccus denitrificans on methanol.1. the dye-linked methanol dehydrogenase from paracoccus denitrificans grown aerobically on methanol has been purified and its properties compared with similar enzymes from other bacteria. it was shown to be specific and to have high affinity for primary alcohols and formaldehyde as substrate, ammonia was the best activator and the enzyme could be linked to reduction of phenazine methosulphate. 2. paracoccus denitrificans could be grown anaerobically on methanol, using nitrate or nitrite as elec ...1978718372
effects induced by rotenone during aerobic growth of paracoccus denitrificans in continuous culture. changes in energy conservation and electron transport associated with nadh dehydrogenase. 1978727852
[no stimulation of the growth of microorganisms by repair-substances (author's transl)].by means of manometric technique the influence of x-ray-repairsubstances on the growth and respiration of micrococcus denitrificans, micrococcus radiodurans and saccharomyces cerevisiae was investigated. though these substances are stimulating the development of higher plants, there is no effect on growth and respiration of these microorganisms.1976798454
acetylene inhibition of nitrous oxide reduction by denitrifying bacteria. 1976817722
[hydrogen and carboxide bacteria belonging to the microflora of degradation].the following bacteria oxidizing hydrogen and/or carbon monoxide were shown by cultivating in the concentration gradient of acetate, lactate, and glucose, to belong to the microflora of dispersal: hydrogenomonas eutropha z-1, h. pantotropha z-11, pseudomonas carboxydoflava z-1107, achromobacter carboxydus z-1171, paracoccus denitrificans, and alcaligenes paradoxus.1976820943
metal accumulation by bacteria with particular reference to dissimilatory sulphate-reducing bacteria.dissimilatory sulphate-reducing bacteria, genera desulfovibrio and desulfotomaculum, exhibit a superior ability, over assimilatory organisms, to extract three amounts of metals from culture media. this property does not appear to be solely a function of the presence of h2s. in media containing elevated amounts of fe, electron dense particles, provisionally identified as fes, are deposited within the cells of dissimilatory bacteria.1976824869
energy conservation during nitrate respiration in paracoccus denitrificans.p/2e ratios were calculated from anaerobic chemostat cultures of paracoccus denitrificans with nitrogenous oxides as electron acceptor. p/2e ratios were calculated, using the ymaxatp values determined for aerobic cultures. when succinate was the carbon and energy source the average p/2e values of the sulphate- and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. with gluconate as carbon and energy source and aver ...1977843167
energy conservation during aerobic growth in paracoccus denitrificans.paracoccus denitrificans was aerobically grown in chemostat culture with succinate or gluconate as carbon source. due to the presence of two phosphorylation sites in the respiratory chain and the absence of branching, theoretical p/o ratios of 1.71 and 1.82 were calculated for cells growing respectively with succinate and gluconate as carbon source. using these data, 95% confidence intervals for the p/o ratio were determined, via a mathematical model, at 0.91-1.15 and 1.00-1.37 for sulphate-limi ...1977843168
numerically dominant denitrifying bacteria from world soils.nineteen soils, three freshwater lake sediments, and oxidized poultry manure were examined to determine the dominant denitrifier populations. the samples, most shown or expected to support active denitrification, were from eight countries and included rice paddy, temperate agricultural, rain forest, organic, and waste-treated soils. over 1,500 organisms that could grow anaerobically on nitrate agar were isolated. after purification, 146 denitrifiers were obtained, as verified by production of n( ...1977869539
localization and stability of hydrogenases from aerobic hydrogen bacteria.alcaligenes eutrophus strains h 16, b 19, g 27 and n9a contained two different hydrogenases. one enzyme catalyzed the reduction of nad by hydrogen and was strictly localized in the soluble cell fraction. while the second enzyme was found to be particulate and unable to react with nad. all other tested strains, alcaligenes paradoxus sa 29, pseudomonas facilis, p. palleronii rh 2, pseudomonas sp. strain ga 3, paracoccus denitrificans, aquaspirillum autotrophicum sa 32, and corynebacterium autotrop ...1977871226
chemical transformation of 4-chloroaniline to a triazene in a bacterial culture medium. 1977881504
primary structure of a high potential iron sulfur protein from a moderately halophilic denitrifying coccus.the occurrence of a specific high potential iron-sulfur protein in a halophilic, denitrifying coccus provisionally assigned to the genus paracoccus has been confirmed through primary structure determination. this protein, known as "hipip", has been found previously only in photosynthetic bacteria. the sequence of this 71-residue protein is as similar to the hipeps from photosynthetic bacteria as they, in turn, are to one another (average number of identically matching residues is 38%). paracoccu ...1977914842
[a physiological and taxonomic study of paracoccus denitrificans (author's transl)]. 1977931260
metabolic regulation of pyruvate kinase isolated from autotrophically and heterotrophically grown paracoccus denitrificans.pyruvate kinase (atp: pyruvate phosphotransferase (ec was partially purified from both autotrophically and heterotrophically grown paracoccus denitrificans. the organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. the enzyme isolated from both sources exhibited sigmoidal kinetics for both phosphoenolpyruvate (pep) and adp. the apparent km for adp and pep in the "autotrophic" enzyme were 0.63 mm adp and 0.25 mm pep. th ...1977931510
a new pathway for the biosynthesis of spermidine. 19761087258
the reversibility of active sulphate transport in membrane vesicles of paracoccus uncoupler-sensitive active transport of sulphate into membrane vesicles prepared from the plasma membrane of paracoccus denitrificans (previously micrococcus denitrificans) can be driven by respiration or by a trans-membrane ph gradient (alkaline inside) generated by the addition either of kcl ( in the presence of nigericin) or of nh4cl. valinomycin does not substitute for nigericin. respiration-driven transport is observed in right-side-out vesicles but not in inside-out vesicles, whereas tr ...19751212205
the autotrophic growth of micrococcus denitrificans on methanol.ribulose bisphosphate carboxylase is present at a high specific activity in extracts of methanol-grown microccus denitrificans. enzymic and physiological evidence indicates that, during growth on methanol, the ribulose bisphosphate cycle is the route of carbon assimilation.19751212208
phosphate transport in membrane vesicles of paracoccus denitrificans. 19751225582
electron transport in aerobically grown paracoccus denitrificans: kinetic characterization of the membrane-bound cytochromes and the stoichiometry of respiration-driven proton translocation. 19761278392
the transport of d-lactate by membrane vesicles from paracoccus denitrificans. 19761278402
comparison of dentrification by paracoccus denitrificans, pseudomonas stutzeri and pseudomonas aeruginosa.the course of denitrification of nitrate, nitrite and both compounds together by static cultures of paracoccus denitrificans, pseudomonas stutzeri and pseudomonas aeruginosa was studied. these strains represent three different types of denitrification: 1. reduction of nitrate to gaseous nitrogen without accumulation of nitrite (p. denitrificans); 2. partial accumulation of nitrite in growing cultures during reduction of nitrate to gaseous nitrogen (p. aeruginosa) and 3. two-phase denitrification ...19921284849
reduction of cua induces a conformational change in cytochrome c oxidase from paracoccus denitrificans.cytochrome c oxidase (cytochrome aa3) from paracoccus denitrificans contains a tightly bound manganese(ii) ion, which responds to reduction of the enzyme by a change in its epr signal (seelig et al. (1981) biochim. biophys. acta 636, 162-167). in this paper, the nature of this phenomenon is studied and the bound manganese is used as a reporter group to monitor a redox-linked conformational change in the protein. a reductive titration of the cyanide-inhibited enzyme shows that the change in the m ...19921310624
cloning, sequencing and deletion from the chromosome of the gene encoding subunit i of the aa3-type cytochrome c oxidase of rhodobacter sphaeroides.the ctad gene encoding subunit i of the aa3-type cytochrome c oxidase from rhodobacter sphaeroides has been cloned. the gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit i from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% identity with that from paracoccus denitrificans. the ctad gene was deleted from the chromosome of r. sphaeroides, resulting in a strain that spectroscopically lacks cytochrome a. this ...19921313140
oxidation of methylamine by a paracoccus denitrificans mutant impaired in the synthesis of the bc1 complex and the aa3-type oxidase. evidence for the existence of an alternative cytochrome c oxidase in this bacterium.a paracoccus denitrificans fbcc-ctadii double mutant strain impaired in the synthesis of both the bc1 complex and the aa3-type oxidase has been constructed. this mutant strain, which is still able to grow on methylamine as sole carbon and energy source, exhibits unimpaired oxygen consumption with succinate, methylamine and endogenous substrates as electron donors. from kinetic studies of the oxidation and reduction rates of cytochromes c, it can be concluded that p. denitrificans contains a seco ...19921321057
terminal oxidases in paracoccus denitrificans. 19921321668
oscillations of nitric oxide concentration in the perturbed denitrification pathway of paracoccus denitrificans.the metabolism of nitric oxide in paracoccus denitrificans has been studied using a clark-type electrode. the uncoupler carbonyl cyanide m-chlorophenylhydrazone (cccp) and the sh reagent n-ethylmaleimide, both of which released nitric oxide from cells respiring nitrite, were found to be efficient inhibitors of nitric oxide reductase activity. control experiments with another uncoupler, pentachlorophenol, showed that the inhibitory effect of cccp was not the result of a decrease in membrane poten ...19921325776
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