pneumonia and meningitis caused by a new nonfermentative unknown gram-negative isolates of an unclassified bacterium resembling flavobacterium spp. were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, protein profile analysis, and serologic data. the unclassified isolates were differentiated from flavobacterium meningosepticum, flavobacterium odoratum, flavobacterium balustinum, flavobacterium strain iib, chromobacterium violaceum, aquaspirillum serpens, and pseudomonas spp. the bacterium ...19892504766
intramuscular imipenem/cilastatin for treatment of mild and moderately severe bacterial infections.the efficacy and safety of intramuscularly administered imipenem/cilastatin was studied in 70 patients with mild or moderately severe bacterial infections (skin and soft tissue infections, respiratory tract infections, urinary tract infections and pelvic infections). doses of imipenem/cilastatin ranged from 0.5 to 0.75 g twice daily. fifty-five patients were evaluable for bacteriological efficacy; in the remaining 15 patients no pathogens were isolated or susceptibility data were lacking. mic50 ...19873483287
the activity of ceftazidime compared with those of aztreonam, newer cephalosporins and sch 29482 against nonfermentative gram-negative bacilli.the activity of ceftazidime was compared with those of aztreonam, newer cephalosporins and sch 29482 against nonfermentative gram-negative bacilli. ceftazidime was consistently more active (mic less than or equal to 8 micrograms) against the nonfermenters. only flavobacterium odoratum, f. spp., pseudomonas alcaligenes, p. maltophilia and p. stutzeri demonstrated substantial resistance (mic90 greater than or equal to 64 micrograms) to ceftazidime. sch 29482 and ceftriaxone also exhibited good act ...19853888543
in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila.the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ...19806448581
bacteremic necrotizing fasciitis due to flavobacterium odoratum. 19958589173
highly conserved neisseria meningitidis surface protein confers protection against experimental infection.a new surface protein, named nspa, which is distinct from the previously described neisseria meningitidis outer membrane proteins was identified. an nspa-specific mab, named me-1, reacted with 99% of the meningococcal strains tested indicating that the epitope recognized by this particular mab is widely distributed and highly conserved. western immunoblotting experiments indicated that mab me-1 is directed against a protein band with an approximate molecular mass of 22,000, but also recognized a ...19979104804
antimicrobial susceptibility of flavobacteria as determined by agar dilution and disk diffusion methods.a total of 106 clinical isolates of flavobacteria, including 41 isolates of flavobacterium meningosepticum, 59 of flavobacterium indologenes, and 6 of flavobacterium odoratum were collected from january 1992 to december 1995 from patients in taiwan. the in vitro activities of antimicrobial agents were determined concomitantly by the standard agar dilution and disk diffusion methods. more than 90% of the flavobacterial isolates were resistant to cephalothin, cefotaxime, ceftriaxone, moxalactam, a ...19979174188
comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ...19979210669
antigenic and molecular conservation of the gonococcal nspa protein.a low-molecular-weight protein named nspa (neisserial surface protein a) was recently identified in the outer membrane of all neisseria meningitidis strains tested. antibodies directed against this protein were shown to protect mice against an experimental meningococcal infection. hybridization experiments clearly demonstrated that the nspa gene was also present in the genomes of the 15 neisseria gonorrhoeae strains tested. cloning and sequencing of the nspa gene of n. gonorrhoeae b2 revealed an ...199910338491
expression of the isoamylase gene of flavobacterium odoratum ku in escherichia coli and identification of essential residues of the enzyme by site-directed mutagenesis.the isoamylase gene from flavobacterium odoratum ku was cloned into and expressed in escherichia coli jm109. the promoter of the gene was successful in e. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. the enzyme found in the culture medium showed almost the same m(r), heat-inactivating constant, and n-terminal sequence as those of the enzyme accumulated in the periplasmic space. this result indicated that the enzyme accumulat ...199910473430
genetic-biochemical analysis and distribution of the ambler class a beta-lactamase cme-2, responsible for extended-spectrum cephalosporin resistance in chryseobacterium (flavobacterium) vitro synergy between extended-spectrum cephalosporins and either clavulanic acid or cefoxitin was found for chryseobacterium meningosepticum isolates during a double-disk assay on an agar plate. an extended-spectrum beta-lactamase (esbl) gene from a c. meningosepticum clinical isolate was cloned and expressed in escherichia coli dh10b. its protein conferred resistance to most beta-lactams including extended-spectrum cephalosporins but not to cephamycins or to imipenem. its activity was stron ...200010602714
evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli.the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ...200010699007
characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france.pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ...200010722487
a canadian national surveillance study of urinary tract isolates from outpatients: comparison of the activities of trimethoprim-sulfamethoxazole, ampicillin, mecillinam, nitrofurantoin, and ciprofloxacin. the canadian urinary isolate study group.ampicillin, trimethoprim-sulfamethoxazole, mecillinam, nitrofurantoin, and ciprofloxacin mean resistance rates for 2,000 urinary tract isolates collected from outpatients across canada in 1998 were 41.1, 19.2, 14.7, 5.0, and 1.8%, respectively. for escherichia coli isolates alone (n = 1,681) comparable rates were 41. 0, 18.9, 7.4, 0.1, and 1.2%, respectively. the majority of e. coli isolates resistant to ampicillin, trimethoprim-sulfamethoxazole, or ciprofloxacin were susceptible (mic, <16 micro ...200010722520
carbapenemases of chryseobacterium (flavobacterium) meningosepticum: distribution of blab and characterization of a novel metallo-beta-lactamase gene, blab3, in the type strain, nctc 10016.genes encoding carbapenemases in 15 reference strains of chryseobacterium (flavobacterium) meningosepticum from the united kingdom national collection of type cultures and in one recent clinical isolate were investigated. all the strains hydrolyzed imipenem, but their levels of resistance to carbapenems varied, with imipenem and meropenem mics ranging from 2 to >32 microg/ml. the blab gene, which encodes a molecular-class b carbapenemase, was detected in only six reference strains and in clinica ...200010817691
the legionella (fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass b3 beta-lactamases.a metallo-beta-lactamase determinant was cloned from a genomic library of legionella (fluoribacter) gormanii atcc 33297(t) constructed in the plasmid vector pacyc184 and transformed into escherichia coli dh5alpha, by screening for clones showing a reduced susceptibility to imipenem. the product of the cloned determinant, named fez-1, contains a 30-kda polypeptide and exhibits an isoelectric ph of 7.6. sequencing revealed that fez-1 is a molecular-class b beta-lactamase which shares the closest s ...200010817705
a novel protein-deamidating enzyme from chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil.a novel protein-deamidating enzyme, which has potential for industrial applications, was purified from the culture supernatant of chryseobacterium proteolyticum strain 9670(t) isolated from rice field soil in tsukuba, japan. the deamidating activities on carboxybenzoxy (cbz)-gln-gly and caseins and protease activity were produced synchronously by the isolate. both deamidating activities were eluted as identical peaks separated from several proteases by phenyl-sepharose chromatography of the cult ...200010919788
application of the 5'-nuclease pcr assay in evaluation and development of methods for quantitative detection of campylobacter jejuni.campylobacter jejuni is recognized as a leading human food-borne pathogen. traditional diagnostic testing for c. jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable state. nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. in this article, we present a 5'-nuclease pcr assay for quantitative detection of c. jejuni and describe its evaluation. a probe including positions ...200010966425
rapid differentiation of fermentative from nonfermentative gram-negative bacilli in positive blood cultures by an impedance method.rapid differentiation of fermentative gram-negative bacilli (fermenters) from nonfermentative gram-negative bacilli (nonfermenters) in positive blood cultures may help physicians to narrow the choice of appropriate antibiotics for empiric treatment. an impedance method for direct differentiation of fermenters from nonfermenters was investigated. the bacterial suspensions (or positive culture broths containing gram-negative bacteria) were inoculated into the module wells of a bactometer (biomérie ...200011015369
psychrobacters and related bacteria in freshwater fish.three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ...200010716558
metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum.eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ...200111181369
rearing stable fly larvae (diptera: muscidae) on an egg yolk medium.the growth and survival of stomoxys calcitrans (l.) larvae on egg yolk medium inoculated with bacteria isolated from a colony of stable flies was evaluated. five species of bacteria--acinetobacter sp., aeromonas sp., empedobacter breve (holmes & owen), flavobacterium odoratum stutzer, and serratia marcescens bizio--were identified according to fatty acid profiles using a microbial identification system. larvae failed to develop on uninoculated plates, confirming that bacteria are required to com ...199910337112
the chryseobacterium meningosepticum pafa enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?chryseobacterium meningosepticum is an aerobic gram-negative rod widely distributed in natural environments. unlike many bacteria, it produces a phosphate-irrepressible periplasmic alkaline phosphatase (ap). this work describes cloning of the gene encoding that enzyme from c. meningosepticum ccug 4310 (nctc 10585), and preliminary characterization of its product. the gene, named pafa, encodes a protein (pafa) of 546 amino acids with a calculated molecular mass of the mature peptide of 58682 da. ...200111577161
myroides odoratus cellulitis and bacteremia: case report and review.a case of myroides odoratus cellulitis with bacteremia in an apparently immunocompetent man is presented. although common in soil and water, this agent is a rare clinical isolate and is often not considered pathogenic. the virulence of m. odoratus may be greater than is currently believed and it should be considered in bacteremias from cutaneous sources in immunocompetent patients.200111868770
ebr-1, a novel ambler subclass b1 beta-lactamase from empedobacter brevis.empedobacter brevis (formerly designated flavobacterium breve) is a gram-negative aerobe involved in nosocomial infections. the ambler class b beta-lactamase gene bla(ebr-1) was cloned and expressed in escherichia coli from e. brevis clinical strain ass-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. purified beta-lactamase ebr-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. kinetic parameters of ebr-1 were similar ...200212234848
chromosome-encoded beta-lactamases tus-1 and mus-1 from myroides odoratus and myroides odoratimimus (formerly flavobacterium odoratum), new members of the lineage of molecular subclass b1 metalloenzymes.myroides odoratus and myroides odoratimimus (formerly designated in a single species as flavobacterium odoratum) are gram-negative aerobes and sources of nosocomial infections in humans. they have variable susceptibility to beta-lactams and a decreased susceptibility to carbapenems. using genomic dnas of m. odoratus cip 103105 and m. odoratimimus cip 103073 reference strains, shotgun cloning of beta-lactamase genes was performed, followed by protein expression in escherichia coli. the deduced am ...200212384365
nosocomial catheter-associated flavobacterium odoratum bacteraemia in cancer patients. 19989879963
use of the bact/alert blood culture system for culture of sterile body fluids other than blood.studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. bact/alert is a fully automated blood culture system for detecting bacteremia and fungemia. in this study, we compared culture in bact/alert standard aerobic and anaerobic bottles, bact/alert fan aerobic and fan anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate ( ...19989774578
genetic diversity of the biofilm covering montacuta ferruginosa (mollusca, bivalvia) as evaluated by denaturing gradient gel electrophoresis analysis and cloning of pcr-amplified gene fragments coding for 16s rrna.the shell of the bivalve montacuta ferruginosa, a symbiont living in the burrow of an echinoid, is covered with a rust-colored biofilm. this biofilm includes different morphotypes of bacteria that are encrusted with a mineral rich in ferric ion and phosphate. the aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria. also, the possible roles of the microorganisms in the processes of mineral deposition within the biofilm, as well as their ...19989726898
rapid detection and evaluation of clinical characteristics of emerging multiple-drug-resistant gram-negative rods carrying the metallo-beta-lactamase gene blaimp.gram-negative rods (gnr) carrying the transferable carbapenem resistance gene blaimp, including pseudomonas aeruginosa and serratia marcescens, have been isolated from more than 20 hospitals in japan. although the emergence of such multiple-drug-resistant bacteria is of utmost clinical concern, little information in regard to the distribution of blaimp-positive gnr in hospitals and the clinical characteristics of infected patients is available. to address this, a system for the rapid detection o ...19989687398
interactions of beta-lactamases with sanfetrinem (gv 104326) compared to those with imipenem and with oral beta-lactams.sanfetrinem is a trinem beta-lactam which can be administered orally as a hexatil ester. we examined whether its beta-lactamase interactions resembled those of the available carbapenems, i.e., stable to ampc and extended-spectrum beta-lactamases but labile to class b and functional group 2f enzymes. the comparator drugs were imipenem, oral cephalosporins, and amoxicillin. mics were determined for beta-lactamase expression variants, and hydrolysis was examined directly with representative enzymes ...19989593145
recurrent cellulitis and bacteremia caused by flavobacterium odoratum. 19968783727
cloning and expression of the unique ca2+-atpase from flavobacterium odoratum.the 60-kda ca2+-atpase from flavobacterium odoratum is kinetically and mechanistically similar to other p-type atpases, suggesting its use as a model system for structure-function studies of ion transport. a portion of the gene was amplified by polymerase chain reaction of genomic dna with degenerate oligonucleotide primers, one based on the n-terminal amino acid sequence of the purified protein and the other based on a consensus sequence for the phosphorylation site of p-type atpases. this gene ...19968617788
phosphorylation and reaction intermediates of the prokaryotic ca(2+)-atpase.a p-type ca(2+)-atpase activity has been identified previously in membranes of the gram-negative bacteria flavobacterium odoratum. the reaction mechanism of the prokaryotic ca(2+)-atpase was examined by isolation of reaction cycle intermediates formed during reversal of the normal cycle using inorganic p(i). the eukaryotic atpase can be directly phosphorylated by p(i) only in the absence of calcium, and half-maximal inhibition is observed at 1-2 microm calcium. the f. odoratum ca(2+)-atpase is a ...19938376412
development of a simple latex agglutination assay for detection of shiga toxin-producing escherichia coli (stec) by using polyclonal antibody against stec.rabbit antiserum raised against the whole-cell antigen of shiga toxin-producing escherichia coli (stec) strain vt3 (stx1+ stx2+ eae+) was repeatedly adsorbed with heat-killed cells of different non-stec strains and other enteric bacteria. thus, the antiserum obtained was designated vt3 antiserum. vt3 antiserum reacted with intimin type gamma. we assessed the reactivity of vt3 antiserum to whole-cell lysates of 87 strains of e. coli and other enteric bacteria by immunoblotting. the antiserum reco ...200717344348
investigation of an outbreak of central venous catheter-associated bloodstream infection due to contaminated outbreak of central venous catheter-associated bloodstream infections was reported in a hospital in ecuador. commercially produced ampoules of water for injection were found to be contaminated with burkholderia cepacia and myroides odoratus. removal of these ampoules yielded a 10-fold reduction in the incidence of catheter-associated infection.200818462150
right-sided bacterial endocarditis due to flavobacterium odoratum in a patient on chronic hemodialysis.bacterial endocarditis, particularly involving the left side, has been shown to occur in patients in regular hemodialysis. we report a case of right-sided endocarditis characterized by a very torpid evolution. although the diagnosis was suspected early in the course, confirmation was obtained 2 months after the onset. flavobacterium odoratum was identified in the fourth month of evolution and only after multiple blood cultures had been obtained. we believe the very low infectivity of f. odoratum ...19957872370
[studies on elastase from flavobacterium. i. strain screening and enzyme purification].132 strains bacteria secreting extracellular elastase were isolated from soil samples, 5 of them possessed considerably high elastiolytic activity of more than 100 u/ml. the highest-yield strain no. 17-87 was characterized as flavobacterium odoratum, studies on the condition of elastase production revealed that its optimum carbohydrate and nitrogen source were glucose and casein respectively, and that it could utilize fowl ferther meal and wheat bran to give 80% relative yield. the culture exhib ...19957725681
flavobacterium odoratum ventriculitis treated with intraventricular cefotaxime.a 6-week-old infant admitted to the university hospital of the west indies with hydrocephalus later developed ventriculitis. a heavy growth of flavobacterium odoratum susceptible to gentamicin and cefotaxime was recovered from the ventricular fluid. since intraventricular therapy was envisaged, a pudenz reservoir was installed and ventricular fluid aspirated every 24 h to monitor treatment. initial therapy consisted of intravenous cefotaxime, 50 mg/kg q.i.d. for 4 days. no significant reduction ...19854086867
ind-6, a highly divergent ind-type metallo-beta-lactamase from chryseobacterium indologenes strain 597 isolated in burkina faso.the genus chryseobacterium and other genera belonging to the family flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. several species of flavobacteriaceae, including chryseobacterium indologenes, are naturally resistant to beta-lactam antibiotics (including carbapenems), due to the production of a resident metallo-beta-lactamase. although c. indologenes presently constitutes a limited clinical threat, the incidence of inf ...200919651915
biochemical properties of beta-lactamase produced by flavobacterium odoratum.a constitutively produced beta-lactamase was purified from flavobacterium odoratum gn14053. the purified enzyme gave a single protein band on polyacrylamide gel electrophoresis. the isoelectric point was 5.8, and the molecular weight was estimated to be about 26,000. the enzyme activity was inhibited by edta, iodine, p-chloromercuribenzoate, hgcl2, and cuso4 but not by clavulanic acid, sulbactam, imipenem, and cephamycin derivatives. the enzyme showed a broad substrate profile, hydrolyzing oxyim ...19853873903
chemical characterization of flavobacterium odoratum, flavobacterium breve, and flavobacterium-like groups iie, iih, and iif.the cellular fatty acid, sphingolipid, and isoprenoid quinone compositions of flavobacterium odoratum, flavobacterium breve, and flavobacterium-like groups iie, iih, and iif were determined, using thin-layer, gas-liquid, and reverse-phase high-performance liquid chromatography. the fatty acid data showed that groups iie, iih, and iif were similar to recognized flavobacterium species by the presence of relatively large amounts of iso-branched hydroxy and nonhydroxy acids. groups iie and iih were ...19863700614
first report of performance of the versant ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae.we evaluated the analytical, work flow, and clinical performance of the versant ct/gc dna 1.0 assay (versant ct/gc assay, where "ct" represents chlamydia trachomatis and "gc" represents neisseria gonorrhoeae). the assay simultaneously detects chlamydia trachomatis and neisseria gonorrhoeae in swab and first-catch urine (fcu) specimens. the limit of detection (lod) was determined to be 342 copies/ml for c. trachomatis and 137 copies/ml for gc. the versant ct/gc assay detected 15 c. trachomatis se ...201121307209
[isolation of flavobacterium odoratum from human matter].described the most important taxonomical, bacteriological, clinical and epidemiological aspects of microorganisms of flavobacterium genus, the author refer about 8 strains of flavobacterium odoratum isolated from urine (6), sputum (1), surgical exudate (1). in some cases, etiopathogenetic possible correlation between isolates and clinical disease discussed, the author emphasizes the large antibiotic resistance kind of this species of microorganism non fermenter.19863615803
biofilm formation and adherence characteristics of an elizabethkingia meningoseptica isolate from oreochromis mossambicus.abstract: background: elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. their ability to exist as biofilm structures has been alluded to but not extensively investigated. methods: the ability of elizabethkingia meningoseptica isolate ch2b from freshwater tilapia (oreochromis mossambicus) and e. meningoseptica strain nctc 10016t to adhere to abiotic surfaces was investigated using microtiter ...201121545730
in vitro activity of imipenem towards gram-negative bacilli.the authors studied the in vitro antimicrobial activity of imipenem towards 355 gram-negative bacterial strains, taking into particular consideration unusual or dangerous species. the study was carried out on a comparative basis with piperacillin, cefotaxime, ceftazidime and gentamicin. ninety percent of the fermentative gram-negative strains were inhibited at concentrations less than or equal to 2 mg/l. imipenem inhibited 100% of strains of alcaligenes faecalis, alcaligenes denitrificans, flavo ...19863466723
purification and characterization of the ca2+-atpase of flavobacterium odoratum.the p-type ca2+-atpase from flavobacterium odoratum has been purified to homogeneity and characterized. inside-out membrane vesicles were extracted with c12e8, followed by ammonium sulfate fractionation, centrifugation through two successive 32-48% glycerol gradients, and de52 ion exchange chromatography. the purified ca2+-atpase consists of a single polypeptide. it migrates electrophoretically with an apparent molecular mass of 60,000 da, consistent with the phosphorylation pattern originally r ...19968632017
molecular detection of bacteria in plant tissues, using universal 16s ribosomal dna degenerated primers.highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. we report here the development of a sensitive polymerase chain reaction (pcr) based method for the rapid detection and identification of bacteria occurring in plant tissue cul ...201426019546
genome-based taxonomic classification of bacteroidetes.the bacterial phylum bacteroidetes, characterized by a distinct gliding motility, occurs in a broad variety of ecosystems, habitats, life styles, and physiologies. accordingly, taxonomic classification of the phylum, based on a limited number of features, proved difficult and controversial in the past, for example, when decisions were based on unresolved phylogenetic trees of the 16s rrna gene sequence. here we use a large collection of type-strain genomes from bacteroidetes and closely related ...201628066339
development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments.previous bacterial transport studies have utilized fluorophores which have been shown to adversely affect the physiology of stained cells. this research was undertaken to identify alternative fluorescent stains that do not adversely affect the transport or viability of bacteria. initial work was performed with a groundwater isolate, comamonas sp. strain da001. potential compounds were first screened to determine staining efficiencies and adverse side effects. 5-(and 6-)-carboxyfluorescein diacet ...200011010903
phylum-wide general protein o-glycosylation system of the bacteroidetes.the human gut symbiont bacteroides fragilis has a general protein o-glycosylation system in which numerous extracytoplasmic proteins are glycosylated at a three amino acid motif. in b. fragilis, protein glycosylation is a fundamental and essential property as mutants with protein glycosylation defects have impaired growth and are unable to competitively colonize the mammalian intestine. in this study, we analysed the phenotype of b. fragilis mutants with defective protein glycosylation and found ...201323551589
ability of the microscan rapid gram-negative id type 3 panel to identify nonenteric glucose-fermenting and nonfermenting gram-negative bacilli.the microscan rapid neg id3 panel is designed for the identification of enterobacteriaceae and nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. we evaluated this panel for its ability to identify gram-negative non-enterobacteriaceae bacteria. a total of 134 strains, representing 26 genera and 42 species, were taken from storage at -70(o)c, passaged three times before testing, and inoculated into the panels according to the manufacturer's directions before being inserted int ...200212354875
flavobacterium odoratum: a species resistant to a wide range of antimicrobial agents.during the period 1966-77, 24 strains of flavobacterium odoratum were identified from among strains of gram-negative, non-fermentative bacteria submitted to the national collection of type cultures for computer-assisted identification. the f. odoratum strains showed resistance to therapeutic levels of gentamicin, tobramycin, amikacin, and carbenicillin as well as to several other antimicrobial agents generally useful in the treatment of infections caused by gram-negative, non-fermentative bacter ...1979429582
[isolation of bacteria designated as "group m-4f" (flavobacterium odoratum) from clinical materials (author's transl)]. 1979427603
comparative activities of clinafloxacin against gram-positive and -negative bacteria.activities of clinafloxacin, ciprofloxacin, levofloxacin, sparfloxacin, trovafloxacin, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ceftazidime, and imipenem against 354 ciprofloxacin-susceptible and -intermediate-resistant organisms were tested by agar dilution. clinafloxacin yielded the lowest quinolone mics (< or = 0.5 microg/ml against ciprofloxacin-susceptible organisms and < or = 16.0 microg/ml against ciprofloxacin-intermediate-resistant organisms) compared to tho ...19989593165
dna-dna hybridization study of strains of chryseobacterium, elizabethkingia and empedobacter and of other usually indole-producing non-fermenters of cdc groups iic, iie, iih and iii, mostly from human clinical sources, and proposals of chryseobacterium bernardetii sp. nov., chryseobacterium carnis sp. nov., chryseobacterium lactis sp. nov., chryseobacterium nakagawai sp. nov. and chryseobacterium taklimakanense comb. nov.the taxonomic classification of 182 phenotypically similar isolates was evaluated using dna-dna hybridization and 16s rrna gene sequence analysis. these bacterial isolates were mainly derived from clinical sources; all were gram-negative non-fermenters and most were indole-producing. phenotypically, they resembled species from the genera chryseobacterium, elizabethkingia or empedobacter or belonged to cdc groups iic, iie, iih and iii. based on these analyses, four novel species are described: ch ...201323934253
aerobic bacterial flora of biotic and abiotic compartments of a hyperendemic zoonotic cutaneous leishmaniasis (zcl) focus.identification of the microflora of the sand fly gut and the environmental distribution of these bacteria are important components for paratransgenic control of leishmania transmission by sand flies.201525630498
gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles.n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ...200011030433
molecular typing of myroides odoratimimus (flavobacterium odoratum) urinary tract infections in a turkish hospital. 200011057514
hospital infestation by the cluster fly, pollenia rudis sensu stricto fabricius 1794 (diptera: calliphoridae), and its possible role in transmission of bacterial pathogens in germany.the potential of the cluster fly, pollenia rudis sensu stricto, to transmit bacterial pathogens was investigated during a mass infestation that took place in a german hospital. cluster flies were individually examined for mesophilic bacteria carried on the exoskeleton. bacterial growth could only be detected by using the enrichment culture technique to increase sensitivity, but not by direct intoculation of fly samples to agar plates. all 50 cluster fly samples that were tested carried opportuni ...200111279815
three isoforms of isoamylase contribute different catalytic properties for the debranching of potato glucans.isoamylases are debranching enzymes that hydrolyze alpha-1,6 linkages in alpha-1,4/alpha-1,6-linked glucan polymers. in plants, they have been shown to be required for the normal synthesis of amylopectin, although the precise manner in which they influence starch synthesis is still debated. cdna clones encoding three distinct isoamylase isoforms (stisa1, stisa2, and stisa3) have been identified from potato. the expression patterns of the genes are consistent with the possibility that they all pl ...200312509527
comparison of the api 20e and bbl crystal e/nf identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (dicentrarchus labrax).the ability of two commercial rapid identification systems, api 20e and bbl crystal e/nf, to reliably identify bacterial isolates from the internal organs of reared sea bass were compared. the tests gave different results: api 20e identified bacteria as pseudomonas spp. with 37% accuracy, while bbl crystal e/nf identified them as flavobacterium odoratum with 99% accuracy. although f. odoratum is not a marine fish pathogen, conventional tests conducted with the same isolates were more indicative ...200414992239
novel sulfonolipid in the extremely halophilic bacterium salinibacter ruber.salinibacter ruber is an extremely halophilic bacterium, phylogenetically affiliated with the flavobacterium/cytophaga branch of the domain bacteria. electrospray mass analyses (negative ion) of the total lipid extract of a pure culture of s. ruber shows a characteristic peak at m/z 660 as the most prominent peak in the high-mass range of the spectrum. a novel sulfonolipid, giving rise to the molecular ion [m-h]- of m/z 660, has been identified. the sulfonolipid isolated and purified by thin-lay ...200415528534
role of the escherichia coli glgx gene in glycogen metabolism.a role for the escherichia coli glgx gene in bacterial glycogen synthesis and/or degradation has been inferred from the sequence homology between the glgx gene and the genes encoding isoamylase-type debranching enzymes; however, experimental evidence or definition of the role of the gene has been lacking. construction of e. coli strains with defined deletions in the glgx gene is reported here. the results show that the glgx gene encodes an isoamylase-type debranching enzyme with high specificity ...200515687211
quantitative detection of clostridium perfringens in the broiler fowl gastrointestinal tract by real-time pcr.strains of clostridium perfringens are a frequent cause of food-borne disease and gas gangrene and are also associated with necrotic enteritis in chickens. to detect and quantify the levels of c. perfringens in the chicken gastrointestinal tract, a quantitative real-time pcr assay utilizing a fluorogenic, hydrolysis-type probe was developed and utilized to assay material retrieved from the broiler chicken cecum and ileum. primers and probe were selected following an alignment of 16s rdna sequenc ...200516000804
[rare bacteria species found in wounds of tsunami patients. predominance of gram-negative rods, increased antibiotic resistance].microbiological cultures from 229 patients seeking medical advise in stockholm after the tsunami catastrophe december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative rods were the most common findings from wound cultures. common human pathogens as escherichia coli, proteus species, klebsiella spp, and pseudomonas aeruginosa were isolated. however, more rare species of gram-negative rods were also isolated, e.g. myroides ...200516416946
[post-traumatic endophthalmitis with multiple water pathogens].a 37-y-old worker for a recycling company suffered from a corneal perforation by a wire which had been in contact with dirt and moisture.19979333666
metallo-beta-lactamases: the quiet before the storm?the ascendancy of metallo-beta-lactamases within the clinical sector, while not ubiquitous, has nonetheless been dramatic; some reports indicate that nearly 30% of imipenem-resistant pseudomonas aeruginosa strains possess a metallo-beta-lactamase. acquisition of a metallo-beta-lactamase gene will invariably mediate broad-spectrum beta-lactam resistance in p. aeruginosa, but the level of in vitro resistance in acinetobacter spp. and enterobacteriaceae is less dependable. their clinical significan ...200515831827
gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe.microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ...200616798691
myroides pelagicus sp. nov., isolated from seawater in aerobic, gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain sm1(t), was isolated from seawater in thailand and subjected to a polyphasic taxonomic study. phylogenetic analyses based on 16s rrna gene sequences revealed that the novel isolate shared 93-95 % sequence similarity with species of the genus myroides. the dna-dna relatedness values of strain sm1(t) with myroides odoratimimus jcm 7460(t) and myroides odoratus jcm 7458(t) were below 70 %. ...200616902031
filifactor alocis--involvement in periodontal biofilms.bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. these highly dynamic microfloral environments challenge both clinicians and researchers alike. the exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. in recent years, filifactor alocis, a fastidious, gram-positive, obligately anaerobic rod was repeatedly identified in periodontal les ...201020193074
evaluation of the wider system, a new computer-assisted image-processing device for bacterial identification and susceptibility testing.the wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. it has been adapted to be able to read and interpret commercial microscan panels. two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family enterobacteriaceae, 25 nonfermentative gram-negative rods [nfgnrs], and 81 gram-positive cocci) were tested. in addition, 100 enterobacterial strains with known beta-lactam re ...200010747104
metal content of metallo-beta-lactamase l1 is determined by the bioavailability of metal an effort to probe whether the metal content of metallo-beta-lactamase l1 is affected by metal ion bioavailability, l1 was overexpressed as mature protein (m-l1) and full-length (fl-l1) analogues, and the analogues were characterized with metal analyses, kinetics, and epr spectroscopy. fl-l1, containing the putative leader sequence, was localized in the periplasm of escherichia coli and shown to bind zn(ii) preferentially. the metal content of fl-l1 could be altered if the enzyme was overexpr ...200818597493
effect of growth temperature and ph on the aminopeptidase activity of pseudomonas putida, p. fluorescens and flavobacterium odoratum; the 4-nitroaniline test is significant difference (p > 0.05) was observed in the specific aminopeptidase activity (saa) developed by pseudomonas fluorescens, p. putida and flavobacterium odoratum either growing at ph 5.0-6.5 or at 7 and 12 degrees c. nevertheless, a significant difference was found when comparing the saa of these organisms. the saa of f. odoratum was lower than those of pseudomonads. the 4-nitroaniline test is reliable to estimate the g(-) load of fresh food products.200819085077
characterization of a p-type ca(2+)-atpase from flavobacterium most bacterial cell types studied, low intracellular free calcium is maintained by a variety of secondary exchangers which utilize transmembrane ion gradients. prokaryotic calcium atpases appear to be extremely uncommon, and none have been reported in gram-negative organisms. we demonstrate atp-dependent calcium uptake in everted membrane vesicles of flavobacterium odoratum, a common gram-negative soil and water bacterium. calcium is transported with an apparent initial rate of 10 nmol/min mg ...19921386366
colonization of an amputation site by flavobacterium odoratum after gentamicin therapy.flavobacterium odoratum was isolated from the stumps of the amputated toes of a debilitated patient. gentamicin therapy and the poor state of health of the patient provided suitable conditions for this gentamicin-resistant species to multiply. when the gentamicin therapy was stopped, and as the patient's heal improved, f. odoratum gradually disappeared to be replaced by staphylococcus aureus. this appears to be the first report of the isolation of f. odoratum in australia, and it indicates the p ...1979530210
detection of extended spectrum beta-lactamases resistance genes among bacteria isolated from selected drinking water distribution channels in southwestern nigeria.extended spectrum beta-lactamases (esbl) provide high level resistance to beta-lactam antibiotics among bacteria. in this study, previously described multidrug resistant bacteria from raw, treated, and municipal taps of dwds from selected dams in southwestern nigeria were assessed for the presence of esbl resistance genes which include bla tem, bla shv, and bla ctx by pcr amplification. a total of 164 bacteria spread across treated (33), raw (66), and municipal taps (68), belonging to α-proteoba ...201627563674
persistence of bacterial indicators and zoonotic pathogens in contaminated cattle wastes.manure can provide a favourable environment for pathogens' survival. de-contamination may be conducted by extended storage, until field conditions are suitable for application to land as source of agricultural nutrients.201627206734
antibiotic resistance mechanisms of myroides sp.bacteria of the genus myroides (myroides spp.) are rare opportunistic pathogens. myroides sp. infections have been reported mainly in china. myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. while 16s ribosomal rna (rrna) gene sequencing can accurately achieve this, it fails to give information on t ...201626984839
imipenem resistance in a flavobacterium odoratum isolate due to class a carbapenemase kpc-2.resistance to carbapenems among enterobacteriaceae strains is mostly due to production of various carbapenemases . the molecular class a carbapenemases of kpc family are an important group in various pathogens. these enzymes hydrolyze all beta-lactams and have been well acknowledged among many members of the family enterobacteriaceae , but rarely reported outside the family enterobacteriaceae. in this report we discribe a flavobacterium odoratum isolate from china that produced carbapenem-hydrol ...201121474611
Nosocomial outbreak of Myroides odoratimimus urinary tract infection in a Tunisian hospital.We report a nosocomial outbreak of urinary tract infection caused by Myroides odoratimimus, previously called Flavobacterium odoratum, in the urology unit of a Tunisian hospital. From May to November 2010, seven isolates of M. odoratimimus were recovered from urine. Pulsed-field gel electrophoresis clearly differentiated these isolates into two possibly related clones from two different periods. All patients but one had urinary calculi and underwent endourological surgery. All Myroides isolates ...201222099498
multiple conversion between the genes encoding bacterial class-i release factors.bacteria require two class-i release factors, rf1 and rf2, that recognize stop codons and promote peptide release from the ribosome. rf1 and rf2 were most likely established through gene duplication followed by altering their stop codon specificities in the common ancestor of extant bacteria. this scenario expects that the two rf gene families have taken independent evolutionary trajectories after the ancestral gene duplication event. however, we here report two independent cases of conversion b ...201526257102
the role of bacterial biofilms and surface components in plant-bacterial associations.the role of bacterial surface components in combination with bacterial functional signals in the process of biofilm formation has been increasingly studied in recent years. plants support a diverse array of bacteria on or in their roots, transport vessels, stems, and leaves. these plant-associated bacteria have important effects on plant health and productivity. biofilm formation on plants is associated with symbiotic and pathogenic responses, but how plants regulate such associations is unclear ...201323903045
electroactivity of phototrophic river biofilms and constitutive cultivable bacteria.electroactivity is a property of microorganisms assembled in biofilms that has been highlighted in a variety of environments. this characteristic was assessed for phototrophic river biofilms at the community scale and at the bacterial population scale. at the community scale, electroactivity was evaluated on stainless steel and copper alloy coupons used both as biofilm colonization supports and as working electrodes. at the population scale, the ability of environmental bacterial strains to cata ...201121642402
septic shock, pneumonia, and soft tissue infection due to myroides odoratimimus: report of a case and review of myroides infections.the genus myroides comprises aerobic, yellow-pigmented, non-motile, non-fermenting gram-negative rods formerly classified as flavobacterium odoratum. members of the genus are widely distributed in the environment, especially in water, and usually behave as low-grade opportunistic pathogens, having been found to cause urinary tract infection, endocarditis, ventriculitis, and cutaneous infections in severely immunocompromised patients. we report a case of soft tissue infection, septic shock, and p ...201121246247
septic shock due to myroides odoratus in a medical intensive care unit patient with severe necrotising pancreatitis. 201627029668
high quality draft genome sequence of flavobacterium rivuli type strain wb 3.3-2(t) (dsm 21788(t)), a valuable source of polysaccharide decomposing enzymes.flavobacterium rivuli ali et al. 2009 emend. dong et al. 2013 is one of about 100 species in the genus flavobacterium (family flavobacteriacae, phylum bacteroidetes) with a validly published name, and has been isolated from the spring of a hard water rivulet in northern germany. including all type strains of the genus myroides and flavobacterium into the 16s rrna gene sequence phylogeny revealed a clustering of members of the genus myroides as a monophyletic group within the genus flavobacterium ...201526380634
polyclonal outbreak of bloodstream infections caused by burkholderia cepacia complex in hematology and bone marrow transplant outpatient units.the objective was to describe an outbreak of bloodstream infections by burkholderia cepacia complex (bcc) in bone marrow transplant and hematology outpatients.201424553612
cloning, sequencing, and in silico analysis of β-propeller phytase bacillus licheniformis strain pb-13.β -propeller phytases (bpphy) are widely distributed in nature and play a major role in phytate-phosphorus cycling. in the present study, a bpphy gene from bacillus licheniformis strain was expressed in e. coli with a phytase activity of 1.15 u/ml and specific activity of 0.92 u/mg proteins. the expressed enzyme represented a full length orf "phypb13" of 381 amino acid residues and differs by 3 residues from the closest similar existing bpphy sequences. the phypb13 sequence was characterized in ...201424864215
identification of new delhi metallo-β-lactamase 1 in acinetobacter lwoffii of food animal investigate the presence of metallo-β-lactamase (mbl) genes and the genetic environment of the new delhi metallo-β-lactamase gene bla(ndm-1) in bacteria of food animal origin.201222629360
mus-2, a novel variant of the chromosome-encoded β-lactamase mus-1, from myroides odoratimimus.the aim of the present study was to investigate the molecular mechanism of carbapenem resistance of three imipenem-resistant isolates of myroides odoratimimus recovered from two livestock farms of cows and pigeons by rectal swab in lebanon in january 2014. investigation of imipenem resistance of these isolates using the modified hodge test, the edta test, the modified carbanp test and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ultraflex assay showed a carbap ...201526257915
passive protection of diabetic rats with antisera specific for the polysaccharide portion of the lipopolysaccharide isolated from pseudomonas pseudomallei.polyclonal and monoclonal antisera raised to tetanus toxoid-conjugated polysaccharide of lipopolysaccharide (lps) and purified lps of pseudomonas pseudomallei that reacted with a collection of 41 strains of this bacterium from 23 patients are described. the common antigen recognized by these sera was within the polysaccharide component of the lps of the cells. the sera were specific for p pseudomallei in that none of 37 strains of other bacteria, including 20 gram-negative and three gram-positiv ...199422346496
comparison of vitek gni and gni+ cards for identification of gram-negative bacteria.the gni+ card has been developed by biomerieux vitek as an improvement over the gni card for the identification of certain species of aerobic and facultative anaerobic bacteria. in this study, we tested 304 organisms from 30 different species on both the gni and gni+ cards. the gni card correctly identified 285 (93.8%) of the isolates tested, and the gni+ card correctly identified 287 (94.4%) of the isolates tested. the average time to reporting was 4.1 h for the gni+ card compared to 5.7 h for ...19989705437
antibacterial activities of multi drug resistant myroides odoratimimus bacteria isolated from adult flesh flies (diptera: sarcophagidae) are independent of metallo beta-lactamase gene.sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. antimicrobial studies of myroides spp. are restricted to nosocomial strains. a gram-negative bacterium, myroides sp., was isolated from the gut of adult flesh flies (sarcophaga sp.) and submitted to evaluation of nutritional parameters using biolog gn, 16s rrna gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of ...200824031236
microbial diversity in a bagasse-based compost prepared for the production of agaricus brasiliensis.edible mushrooms are renowned for their nutritional and medicinal properties and are thus of considerable commercial importance. mushroom production depends on the chemical composition of the basic substrates and additional supplements employed in the compost as well as on the method of composting. in order to minimise the cost of mushroom production, considerable interest has been shown in the use of agro-industrial residues in the preparation of alternative compost mixtures. however, the inter ...200924031404
equine keratomycoses in california from 1987 to 2010 (47 cases).equine keratomycosis in the western usa has received little study, probably owing to its low prevalence.201222943420
equine keratomycoses in california from 1987 to 2010 (47 cases).equine keratomycosis in the western usa has received little study, probably owing to its low prevalence.201222943420
assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.ventilator-associated pneumonia (vap) is a common nosocomial infection in mechanically ventilated patients. biofilm formation is one of the mechanisms through which the endotracheal tube (et) facilitates bacterial contamination of the lower airways. in the present study, we analyzed the composition of the et biofilm flora by means of culture dependent and culture independent (16 s rrna gene clone libraries and pyrosequencing) approaches. overall, the microbial diversity was high and members of d ...201222693635
a positive correlation between bacterial autoaggregation and biofilm formation in native sinorhizobium meliloti isolates from argentina.sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. s. meliloti produces two exopolysaccharides (epss), termed eps i and eps ii, that are both able to promote symbiosis. eps i and eps ii are secreted in two major fractions that reflect differing degrees of subunit polymerization, designated high- and low-molecular-weight fractions. we reported previously that epss are crucial for autoaggregation and biofilm formation in s. mel ...201222492433
calcium homeostasis in pseudomonas aeruginosa requires multiple transporters and modulates swarming motility.pseudomonas aeruginosa is an opportunistic human pathogen causing severe acute and chronic infections. earlier we have shown that calcium (ca(2+)) induces p. aeruginosa biofilm formation and production of virulence factors. to enable further studies of the regulatory role of ca(2+), we characterized ca(2+) homeostasis in p. aeruginosa pao1 cells. by using ca(2+)-binding photoprotein aequorin, we determined that the concentration of free intracellular ca(2+) ([ca(2+)]in) is 0.14±0.05μm. in respon ...201324074964
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