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l-lysine-alpha-ketoglutarate epsilon-aminotransferease. properties of the bound pyridoxal 5'-phosphate.l-lysine-alpha-ketoglutarate epsilon-aminotransferase of flavobacterium lutescens (achromobacter liquidum) ifo 3084 shows positive circular dichroic bands at 340 and 415 nm where absorption maxima are observed, and fluorescence maxima at 380 and 490 nm on excitation at 340 and 415 nm, respectively. the pyridoxal 5'-phosphate absorbing at 415 nm is bound through an aldimine linkage to an epsilon-amino group of the lysine residue of the protein. upon aging, the 415 nm pyridoxal 5'-phosphate change ...1977914795
extracellular product of nocardia amarae induces bacterial cell flocculation.the fact that nocardia amarae yk1 produced a bacterial flocculation-inducing substance (designated as fix) was discovered. fix had a function of flocculating proliferous cells. fix-induced flocculation was inhibited by making cells resting, but not completely by adding chloramphenicol. fix worked widely on gram-positive to -negative bacteria. in the presence of fix, achromobacter cycloclastus iam1013, acinetobacter calcoaceticus iam1517, bacillus subtilis iam1069, escherichia coli c600-1, e. col ...19892653957
l-lysine transaminase from flavobacterium lutescens. 19854088080
use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates.a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ...19937504429
the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species.the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ...19958587109
a highly selective pcr protocol for detecting 16s rrna genes of the genus pseudomonas (sensu stricto) in environmental samples.pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. specific detection of pseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. the objective of this study was to develop a pcr protocol for selective detection of pseudomonas (sensu stricto) in envir ...19989647828
distribution of aldoxime dehydratase in microorganisms.the distribution of phenylacetaldoxime-degrading and pyridine-3-aldoxime-degrading ability was examined with intact cells of 975 microorganisms, including 45 genera of bacteria, 11 genera of actinomyces, 22 genera of yeasts, and 37 genera of fungi, by monitoring the decrease of the aldoximes by high-pressure liquid chromatography. the abilities were found to be widely distributed in bacteria, actinomyces, fungi, and some yeasts: 98 and 107 strains degraded phenylacetaldoxime and pyridine-3-aldox ...200010831401
characterization of l-lysine 6-aminotransferase and its structural gene from flavobacterium lutescens ifo3084.l-lysine 6-aminotransferase (lat) is an enzyme involved in l-lysine catabolism in a wide range of living organisms. lat from flavobacterium lutescens ifo3084 was purified, and its structural gene (lat) was cloned, sequenced and expressed in escherichia coli. native page analysis of purified lat gave a single band corresponding to a molecular weight of about 110,000. lat encoded a protein of 493 amino acids with a deduced molecular weight of 53,200, which is very close to that of purified lat det ...200010965037
composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons.soil contaminated with c5+, which contained benzene (45%, wt/wt), dicyclopentadiene (dcpd) plus cyclopentadiene (together 20%), toluene (6%), styrene (3%), xylenes (2%), naphthalene (2%), and smaller quantities of other compounds, served as the source for isolation of 55 genomically distinct bacteria (standards). use of benzene as a substrate by these bacteria was most widespread (31 of 44 standards tested), followed by toluene (23 of 44), xylenes (14 of 44), styrene (10 of 44), and naphthalene ...200011097903
cloning and characterization of pcd encoding delta'-piperideine-6-carboxylate dehydrogenase from flavobacterium lutescens ifo3084.the pcd gene from flavobacterium lutescens ifo3084 encoding delta'-piperideine-6-carboxylate dehydrogenase (pcd) was cloned, sequenced, and expressed in escherichia coli. the deduced amino acid sequence of pcd from f. lutescens ifo3084 showed strong similarity to that from streptomyces clavuligerus. the molecular mass of the recombinant pcd was estimated to be approximately 58,000 da by sds-page and native page, which indicated that the enzyme molecule is a monomer. the in vitro analysis of l-al ...200011098140
multiple and interconnected pathways for l-lysine catabolism in pseudomonas putida kt2440.l-lysine catabolism in pseudomonas putida kt2440 was generally thought to occur via the aminovalerate pathway. in this study we demonstrate the operation of the alternative aminoadipate pathway with the intermediates d-lysine, l-pipecolate, and aminoadipate. the simultaneous operation of both pathways for the use of l-lysine as the sole carbon and nitrogen source was confirmed genetically. mutants with mutations in either pathway failed to use l-lysine as the sole carbon and nitrogen source, alt ...200516237033
identification of hydrocarbon-degrading bacteria in soil by reverse sample genome probing.bacteria with limited genomic cross-hybridization were isolated from soil contaminated with c5+, a mixture of hydrocarbons, and identified by partial 16s rrna sequencing. filters containing denatured genomic dnas were used in a reverse sample genome probe (rsgp) procedure for analysis of the effect of an easily degradable compound (toluene) and a highly recalcitrant compound (dicyclopentadiene [dcpd]) on community composition. hybridization with labeled total-community dna isolated from soil exp ...199816349504
biology of pseudomonas stutzeri.pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. the species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many ...200616760312
pcd mutants of streptomyces clavuligerus still produce cephamycin c.biosynthesis of cephamycin c in streptomyces clavuligerus involves the initial conversion of lysine to alpha-aminoadipic acid. lysine-6-aminotransferase and piperideine-6-carboxylate dehydrogenase carry out this two-step reaction, and genes encoding each of these enzymes are found within the cephamycin c gene cluster. however, while mutation of the lat gene causes complete loss of cephamycin production, pcd mutants still produce cephamycin at 30% to 70% of wild-type levels. cephamycin production ...200717573474
biofouling characteristics using flow field-flow fractionation: effect of bacteria and membrane properties.in this study, membrane biofouling caused by bacteria that have different characteristics was evaluated using flow field-flow fractionation (flfff). three different bacteria which differed from size and shape (staphylococcus epidermidis, escherichia coli, flavobacterium lutescens) were investigated with gm ultrafiltration (uf, rough with a low negative surface charge and relatively high hydrophobicity) and ne70 nanofiltration (nf, smooth with a high negative surface charge and relatively low hyd ...201019735999
the genome of undifilum oxytropis provides insights into swainsonine biosynthesis and locoism.undifilum oxytropis is a fungal endophyte of locoweeds. it produces swainsonine, which is the principal toxic ingredient of locoweeds. however, the genes, pathways and mechanisms of swainsonine biosynthesis are not known. in this study, the genome of u. oxytropis was firstly sequenced and assembled into a 70.05 megabases (mb) draft genome, which encoded 11,057 protein-coding genes, and 54% of them were similar to current publicly available sequences. u. oxytropis genes were annotated and 164 put ...201627477109
effects offlavobacterium lutescens growth on populations ofescherichia coli andstreptococcus faecalis in water following thermal loading.flavobacterium lutescens has been observed to constitute a major segment of the aerobic, heterotrophic bacterial populations in nonpolluted aquatic systems. it is present in lesser numbers in the presence of municipal sewage and higher concentrations of organic wastes. in laboratory tests, in water from nonpolluted systems, this species became the predominant bacterial type following thermal addition. when temperature was increased in water from polluted sources,f. lutescens became a major compo ...197524241333
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