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riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group.the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ...19938240957
new degenerate cytophaga-flexibacter-bacteroides-specific 16s ribosomal dna-targeted oligonucleotide probes reveal high bacterial diversity in river taff epilithon.river microbial communities play an important role in global nutrient cycles, and aggregated bacteria such as those in epilithic biofilms may be major contributors. in this study the bacterial diversity of river taff epilithon in south wales was investigated. a 16s ribosomal dna (rdna) clone library was constructed and analyzed by partial sequencing of 76 of 347 clones and hybridization with taxon-specific probes. the epilithon was found to be very diverse, with an estimated 59.6% of the bacteri ...200211772628
chryseobacterium defluvii sp. nov., isolated from wastewater.a gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterium (strain b2t) isolated from wastewater of a sequence batch reactor showing enhanced phosphorus removal was investigated to determine its taxonomic status. complete 16s rrna gene sequence analysis indicated that the organism should be placed in the genus chryseobacterium. the strain contained a polyamine pattern with sym-homospermidine as the major compound, menaquinone mk-6 as the predominant menaquinone and ai-c15:0, i-c1 ...200312656158
identification of the bacterial community of maple sap by using amplified ribosomal dna (rdna) restriction analysis and rdna sequencing.the bacterial community of maple sap was characterized by analysis of samples obtained at the taphole of maple trees for the 2001 and 2002 seasons. among the 190 bacterial isolates, 32 groups were formed according to the similarity of the banding patterns obtained by amplified ribosomal dna restriction analysis (ardra). a subset of representative isolates for each ardra group was identified by 16s rrna gene fragment sequencing. results showed a wide variety of organisms, with 22 different genera ...200415066796
chryseobacterium formosense sp. nov., isolated from the rhizosphere of lactuca sativa l. (garden lettuce).a yellow-pigmented bacterial strain (cc-h3-2t), isolated from the rhizosphere of lactuca sativa l. (garden lettuce) in taiwan, was investigated using a polyphasic taxonomic approach. the cells were gram-negative, rod-shaped and non-spore-forming. phylogenetic analyses using the 16s rrna gene sequence of the isolate indicated that the organism belongs to the genus chryseobacterium, with the highest sequence similarity to the type strains of chryseobacterium indoltheticum (97.7 %), chryseobacteriu ...200515653912
chryseobacterium soldanellicola sp. nov. and chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants.two gram-negative, yellow-pigmented bacteria designated psd1-4t and pha3-4t, isolated from two sand-dune plant species inhabiting coastal areas in tae-an, korea, were subjected to taxonomic investigation. 16s rrna gene sequence analysis indicated that both isolates should be placed in the genus chryseobacterium of the family flavobacteriaceae. the phenotypic properties of the strains were also consistent with their classification into this genus. the levels of 16s rrna gene sequence similarity b ...200616449453
influence of lipopolysaccharides and lipids a from some marine bacteria on spontaneous and escherichia coli lps-induced tnf-alpha release from peripheral human blood cells.some endotoxic properties of lipopolysaccharides (lps) and lipids a (la) from the marine bacteria marinomonas communis atcc 27118(t), marinomonas mediterranea atcc 700492(t), and chryseobacterium indoltheticum cip 103168(t) were studied. the preparations tested were shown to have high 50% lethal doses (4 microg per mouse for lps from m. mediterranea and more than 12 microg per mouse for two other lps and la from c. indoltheticum) and were moderate (371 +/- 37 pg/ml at 10 microg/ml of c. indolthe ...200616903830
molecular structure of endotoxins from gram-negative marine bacteria: an update.marine bacteria are microrganisms that have adapted, through millions of years, to survival in environments often characterized by one or more extreme physical or chemical parameters, namely pressure, temperature and salinity. the main interest in the research on marine bacteria is due to their ability to produce several biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents. nonetheless, lipopolysaccharides (lpss), or their portions, from g ...200718463721
effects of bovine milk lactoperoxidase system on some bacteria.bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ...201020198919
gram-negative marine bacteria: structural features of lipopolysaccharides and their relevance for economically important diseases.gram-negative marine bacteria can thrive in harsh oceanic conditions, partly because of the structural diversity of the cell wall and its components, particularly lipopolysaccharide (lps). lps is composed of three main parts, an o-antigen, lipid a, and a core region, all of which display immense structural variations among different bacterial species. these components not only provide cell integrity but also elicit an immune response in the host, which ranges from other marine organisms to human ...201424796306
dna-dna hybridization study of strains of chryseobacterium, elizabethkingia and empedobacter and of other usually indole-producing non-fermenters of cdc groups iic, iie, iih and iii, mostly from human clinical sources, and proposals of chryseobacterium bernardetii sp. nov., chryseobacterium carnis sp. nov., chryseobacterium lactis sp. nov., chryseobacterium nakagawai sp. nov. and chryseobacterium taklimakanense comb. nov.the taxonomic classification of 182 phenotypically similar isolates was evaluated using dna-dna hybridization and 16s rrna gene sequence analysis. these bacterial isolates were mainly derived from clinical sources; all were gram-negative non-fermenters and most were indole-producing. phenotypically, they resembled species from the genera chryseobacterium, elizabethkingia or empedobacter or belonged to cdc groups iic, iie, iih and iii. based on these analyses, four novel species are described: ch ...201323934253
metagenomic analysis of streptomyces lividans reveals host-dependent functional expression.most functional metagenomic studies have been limited by the poor expression of many genes derived from metagenomic dna in escherichia coli, which has been the predominant surrogate host to date. to expand the range of expressed genes, we developed tools for construction and functional screening of metagenomic libraries in streptomyces lividans. we expanded on previously published protocols by constructing a system that enables retrieval and characterization of the metagenomic dna from biologica ...201222427497
plant growth promotion potential is equally represented in diverse grapevine root-associated bacterial communities from different biopedoclimatic environments.plant-associated bacteria provide important services to host plants. environmental factors such as cultivar type and pedoclimatic conditions contribute to shape their diversity. however, whether these environmental factors may influence the plant growth promoting (pgp) potential of the root-associated bacteria is not widely understood. to address this issue, the diversity and pgp potential of the bacterial assemblage associated with the grapevine root system of different cultivars in three medit ...201323878810
contamination of sulfonamide antibiotics and sulfamethazine-resistant bacteria in the downstream and estuarine areas of jiulong river in southeast china.surface water samples from downstream and estuarine areas of jiulong river were collected in august 2011 and may 2012 for detecting sulfonamide antibiotic residues and isolating sulfamethazine-resistant bacteria. sulfamethazine was detected in all samples in may 2012 at an average concentration of 78.3 ng l(-1), which was the highest among the nine sulfonamide antibiotics determined. sulfamethazine-resistant bacteria (srb) were screened using antibiotic-containing agar plates. the srb average ab ...201525877900
community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees.cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ...201423893311
chryseobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a rhus vernicifera-cultivated field.a gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated thg 15(t), was isolated from soil of a field cultivated with rhus vernicifera in okcheon province, south korea, and its taxonomic position was investigated by using a polyphasic approach. strain thg 15(t) grew optimally at 25-30 °c and at ph 7 in the absence of nacl on nutrient agar. strain thg 15(t) displayed β-glucosidase (aesculinase) activity that was responsible for its ability ...201020656821
chryseobacterium aquaticum sp. nov., isolated from a water reservoir.strains 10-46(t) and 10-106 were isolated from a water reservoir in korea. they were gram-negative, non-motile, non-spore-forming rods, produced yellow-pigmented colonies on nutrient agar, contained mk-6 as the predominant menaquinone and 15 : 0 iso and 17 : 0 iso 3-oh as the major fatty acids and had polar lipid profiles consisting of phosphatidylethanolamine and several unknown aminolipids. phylogenetic analysis, based on 16s rrna gene sequencing, showed that the strains were closely related t ...200818319450
chryseobacterium taihuense sp. nov., isolated from a eutrophic lake, and emended descriptions of the genus chryseobacterium, chryseobacterium taiwanense, chryseobacterium jejuense and chryseobacterium indoltheticum.bacterial strain thmbm1(t) was isolated from decomposing algal scum that was collected during an algal bloom in lake taihu, wuxi city, jiangsu province, china. cells of strain thmbm1(t) were gram-negative, facultatively anaerobic, non-motile rods. colonies on tryptic soy agar were translucent and shiny with entire edges; yellow flexirubin-type pigments were produced. growth was observed at 15-45 °c (optimum 30-37 °c), at ph 5.0-9.0 (optimum ph 8.0) and in the presence of 0-4.0 % (w/v) nacl (opti ...201322634705
chryseobacterium ginsengisoli sp. nov., isolated from the rhizosphere of ginseng and emended description of chryseobacterium gleum.strain dcy 63(t), isolated from the rhizosphere of ginseng, was a gram-reaction-negative, strictly aerobic, non-motile, catalase- and oxidase-positive rod. 16s rrna gene sequence analysis revealed that strain dcy 63(t) belonged to the genus chryseobacterium and was closely related to chryseobacterium indoltheticum lmg 4025(t) (98.1 %), chryseobacterium ginsenosidimutans thg 15(t) (98.0 %) and chryseobacterium scophthalmum lmg 13028(t) (97.1 %). the major polar lipids of strain dcy 63(t) were pho ...201323378110
chryseobacterium solani sp. nov., isolated from field-grown eggplant rhizosphere soil.strain thg-ep9t, a gram-stain-negative, aerobic, motile, rod-shaped bacterium was isolated from field-grown eggplant (solanum melongena) rhizosphere soil collected in pyeongtaek, gyeonggi-do, republic of korea. based on 16s rrna gene sequence comparisons, strain thg-ep9t had closest similarity with chryseobacterium ginsenosidimutans thg 15t (97.3 % 16s rrna gene sequence similarity), chryseobacterium soldanellicola psd1-4t (97.2%), chryseobacterium zeae jm-1085t (97.2%) and chryseobacterium indo ...201525878202
chryseobacterium formosus sp. nov., a bacterium isolated from an ancient tree trunk.a gram-reaction-negative, non-motile and rod-shaped bacterium, designated as thg-dn3.6(t), was isolated from an ancient tree trunk from republic of korea. on the basis of 16s rrna gene sequence analysis, strain thg-dn3.6(t) was shown to belong to the genus chryseobacterium and the highest similarity to chryseobacterium indoltheticum lmg 4025(t) (97.2%) and the closest phylogenetic relatives were chryseobacterium scophthalmum (96.8%), chryseobacterium piscium (96.7%) and chryseobacterium balustin ...201526210236
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