| evaluation of the 4-hour rapid nf plus method for identification of 345 gram-negative nonfermentative rods. | the ability of the rapid nf plus system (innovative diagnostic systems, inc., atlanta, ga.) to identify 345 nonfermentative gram-negative rods was evaluated. kits were inoculated with no. 1 mcfarland suspensions, and reactions were interpreted after a 4-h incubation at 35 degrees c. overall, the method correctly identified 311 strains (90.1%) without additional tests and 21 strains (6.1%) with additional tests, and 13 strains (3.8%) were misidentified. five of 13 misidentified strains were alcal ... | 1992 | 1583129 |
| genotypic and phenotypic differentiation of flavobacterium indologenes yabuuchi et al 1983 from flavobacterium gleum holmes et al 1984. | the type and eight strains of flavobacterium indologenes were clearly differentiated from the type and two reference strains of flavobacterium gleum by deoxyribonucleic acid-deoxyribonucleic acid homology data and phenotypic characteristics. phenotypic characteristics useful to differentiate the two species are presented. | 1990 | 2325581 |
| riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the flavobacterium-cytophaga rrna homology group. | the phylogenetic position of the causative agent of septicemia anserum exsudativa, now most often referred to as [moraxella] anatipestifer (brackets indicate a generically misnamed taxon) or "[pasteurella] anatipestifer," was established by performing rrna cistron similarity studies. [moraxella] anatipestifer belongs to rrna superfamily v, together with the genera flavobacterium, cytophaga, flexibacter, weeksella, capnocytophaga, and sphingobacterium. the detailed structure of rrna superfamily v ... | 1993 | 8240957 |
| phylogenetic position of riemerella anatipestifer based on 16s rrna gene sequences. | riemerella anatipestifer, the causative agent of septicemia anserum exsudativa (also called new duckling disease), belongs to the family flavobacteriaceae of gram-negative bacteria. we determined the dna sequences of the rrs genes encoding the 16s rrnas of four r. anatipestifer strains by directly sequencing pcr-amplified rrs genes. a sequence similarity analysis confirmed the phylogenetic position of r. anatipestifer in the family flavobacteriaceae in rrna superfamily v and allowed fine mapping ... | 1997 | 9103649 |
| molecular and biochemical heterogeneity of class b carbapenem-hydrolyzing beta-lactamases in chryseobacterium meningosepticum. | although the carbapenem-hydrolyzing beta-lactamase (chbetal) blab-1 is known to be in chryseobacterium meningosepticum nctc 10585, a second chbetal gene, bla(gob-1), was cloned from another c. meningosepticum clinical isolate (pint). the g+c content of bla(gob-1) (36%) indicated the likely chromosomal origin of this gene. its expression in escherichia coli dh10b yields a mature chbetal with a pi of 8.7 and a relative molecular mass of 28.2 kda. in e. coli, gob-1 conferred resistance to narrow-sp ... | 2000 | 10858348 |
| a novel protein-deamidating enzyme from chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. | a novel protein-deamidating enzyme, which has potential for industrial applications, was purified from the culture supernatant of chryseobacterium proteolyticum strain 9670(t) isolated from rice field soil in tsukuba, japan. the deamidating activities on carboxybenzoxy (cbz)-gln-gly and caseins and protease activity were produced synchronously by the isolate. both deamidating activities were eluted as identical peaks separated from several proteases by phenyl-sepharose chromatography of the cult ... | 2000 | 10919788 |
| genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from chryseobacterium (flavobacterium) indologenes. | the class b carbapenem-hydrolyzing beta-lactamase ind-1 has been characterized for chryseobacterium indologenes strain 001. with internal primers for the bla gene for ind-1 (bla(ind-1)) and an internal bla(ind-1) probe, pcr amplifications failed, while hybridization results were positive when dna from another c. indologenes isolate, strain cip101026, was used as a template. thus, a bla(ind)-related gene was cloned from this c. indologenes reference strain. sequencing of the insert of a recombina ... | 2000 | 11036018 |
| molecular and biochemical characterization of ambler class a extended-spectrum beta-lactamase cga-1 from chryseobacterium gleum. | antibiotic susceptibility testing by disk diffusion of a chryseobacterium gleum isolate, strain cip 103039, showed a typical synergy image between clavulanic acid and expanded-spectrum cephalosporins. shotgun cloning gave a recombinant plasmid in escherichia coli that produced a beta-lactamase, cga-1, with a pi value of 8.9 that conferred resistance to most penicillins (except ureidopenicillins) and narrow-spectrum cephalosporins and an intermediate susceptibility to expanded-spectrum cephalospo ... | 2002 | 11897576 |
| extensive profiling of a complex microbial community by high-throughput sequencing. | complex microbial communities remain poorly characterized despite their ubiquity and importance to human and animal health, agriculture, and industry. attempts to describe microbial communities by either traditional microbiological methods or molecular methods have been limited in both scale and precision. the availability of genomics technologies offers an unprecedented opportunity to conduct more comprehensive characterizations of microbial communities. here we describe the application of an e ... | 2002 | 12039767 |
| inhibition of bacterial alpha-glucosidases by castanospermine in pure cultures and activated sludge. | castanospermine (cast) is a known and potent inhibitor of various alpha-glucosidases in eukaryotes. in this work, we elucidated whether cast could also be used for determining bacterial alpha-glucosidase activity, when measured with 4-methylumbelliferyl-alpha- d-glucoside as a substrate, both in a complex bacterial community, in activated sludge and in pure cultures of bacterial isolates. we found that 140 microm cast inhibited alpha-glucosidase activity by 30% in a pure culture of pseudomonas s ... | 2002 | 12073134 |
| genetic and biochemical characterization of cgb-1, an ambler class b carbapenem-hydrolyzing beta-lactamase from chryseobacterium gleum. | chryseobacterium gleum (previously included in the flavobacterium iib species) is a gram-negative aerobe that is a source of nosocomial infections. an ambler class b beta-lactamase gene was cloned and expressed in escherichia coli from reference strain c. gleum cip 103039 that had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. the purified beta-lactamase, cgb-1, with a pi value of 8.6 and a determined relative molecular mass of ca. 26 kda, hydrolyzed penicillins; nar ... | 2002 | 12183230 |
| ebr-1, a novel ambler subclass b1 beta-lactamase from empedobacter brevis. | empedobacter brevis (formerly designated flavobacterium breve) is a gram-negative aerobe involved in nosocomial infections. the ambler class b beta-lactamase gene bla(ebr-1) was cloned and expressed in escherichia coli from e. brevis clinical strain ass-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. purified beta-lactamase ebr-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. kinetic parameters of ebr-1 were similar ... | 2002 | 12234848 |
| chryseobacterium defluvii sp. nov., isolated from wastewater. | a gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterium (strain b2t) isolated from wastewater of a sequence batch reactor showing enhanced phosphorus removal was investigated to determine its taxonomic status. complete 16s rrna gene sequence analysis indicated that the organism should be placed in the genus chryseobacterium. the strain contained a polyamine pattern with sym-homospermidine as the major compound, menaquinone mk-6 as the predominant menaquinone and ai-c15:0, i-c1 ... | 2003 | 12656158 |
| antimicrobial susceptibility and epidemiology of a worldwide collection of chryseobacterium spp: report from the sentry antimicrobial surveillance program (1997-2001). | limited data are available on chryseobacterium spp. leading to an evaluation of the patient demographics and susceptibility patterns for chryseobacterium spp. collected in the first 5 years of the sentry antimicrobial surveillance program (1997 to 2001). fifty isolates (24 chryseobacterium meningosepticum, 20 chryseobacterium indologenes, two chryseobacterium gleum, and 4 chryseobacterium spp. isolates) were collected. the highest chryseobacterium prevalence was detected among the elderly. the m ... | 2004 | 14715802 |
| chryseobacterium indologenes non-catheter-related bacteremia in a patient with a solid tumor. | a case of non-catheter-related bacteremia caused by chryseobacterium indologenes in a nonneutropenic man with a solid tumor is described. the patient was successfully treated with piperacillin-tazobactam. | 2005 | 15815050 |
| metallo-beta-lactamases: the quiet before the storm? | the ascendancy of metallo-beta-lactamases within the clinical sector, while not ubiquitous, has nonetheless been dramatic; some reports indicate that nearly 30% of imipenem-resistant pseudomonas aeruginosa strains possess a metallo-beta-lactamase. acquisition of a metallo-beta-lactamase gene will invariably mediate broad-spectrum beta-lactam resistance in p. aeruginosa, but the level of in vitro resistance in acinetobacter spp. and enterobacteriaceae is less dependable. their clinical significan ... | 2005 | 15831827 |
| chryseobacterium taichungense sp. nov., isolated from contaminated soil. | a bacterial strain (cc-twgs1-8(t)) isolated from a tar-contaminated soil in taiwan was studied in a detailed taxonomic study. the cells were gram-negative, rod-shaped and non-spore-forming. phylogenetic analyses using the 16s rrna gene sequence of the strain clearly revealed an affiliation to the genus chryseobacterium, the highest sequence similarities being to the type strain of chryseobacterium indologenes (96.8 %), to chryseobacterium gleum (96.8 %) and to chryseobacterium joostei (96.4 %). ... | 2005 | 15879271 |
| chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. | yellow-pigmented, gram-negative organisms isolated from raw chicken were investigated by means of a polyphasic taxonomic approach and were shown to represent a novel species in the genus chryseobacterium, for which the name chryseobacterium vrystaatense sp. nov. is proposed. its nearest phylogenetic neighbours were chryseobacterium joostei, chryseobacterium indologenes and chryseobacterium gleum, which showed 16s rrna gene sequence similarity levels of 96.9, 97.1 and 96.1%, respectively. levels ... | 2005 | 16166723 |
| tla-2, a novel ambler class a expanded-spectrum beta-lactamase. | beta-lactamase tla-2 is encoded by a 47-kb plasmid isolated from an unidentified bacterial strain from a wastewater treatment plant. tla-2 is an ambler class a beta-lactamase that shares 52% amino acid identity with cga-1 from chryseobacterium gleum and 51% with tla-1 from escherichia coli. the enzyme hydrolyzes mostly cephalosporins. | 2005 | 16251326 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system. | a non-motile, yellow-pigmented bacterium, designated strain cw9(t), was isolated from a water-cooling system in gwangyang, republic of korea. the cells were gram-negative, catalase- and oxidase-positive, short rods. the major fatty acids were iso-c(15 : 0) (45.5 %), iso-c(17 : 1)omega9c (14.4 %), iso-c(17 : 0) 3-oh (13.0 %) and summed feature 3 (comprising iso-c(15 : 0) 2-oh and/or c(16 : 1)omega7c; 8.8 %). the dna g+c content was 35.6 mol%. a phylogenetic tree based on 16s rrna gene sequences s ... | 2008 | 18319464 |
| chemotaxonomy of bacteria by comprehensive gc and gc-ms in electron impact and chemical ionisation mode. | the analysis of the cellular lipidic fraction of bacteria is described. after hydrolysis and methylation, the fatty acid methyl esters (fames) are determined by 1-d gc using the sherlock midi bacteria identification system, by comprehensive gc (gc x gc) and by gc-ms in electron impact (ei) and positive chemical ionisation (pci) mode. with gc x gc, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. gc-ei-ms and gc-pci-ms w ... | 2008 | 18792008 |
| effect of potting mix microbial carrying capacity on biological control of rhizoctonia damping-off of radish and rhizoctonia crown and root rot of poinsettia. | abstract potting mixes prepared with dark, highly decomposed sphagnum peat, with light, less decomposed sphagnum peat, or with composted pine bark, all three of which were colonized by indigenous microorganisms, failed to consistently suppress rhizoctonia damping-off of radish or rhizoctonia crown and root rot of poinsettia. inoculation of these mixes with chryseobacterium gleum (c(299)r(2)) and trichoderma hamatum 382 (t(382)) significantly reduced the severity of both diseases in the composted ... | 2001 | 18943449 |
| characteristics of sulfobacin a from a soil isolate chryseobacterium gleum. | a nonmotile, nonspore-forming, gram-negative, aerobic, small rod-shaped bacterium, isolated from soil, was identified as chryseobacterium gleum on the basis of 16s rrna gene sequence analysis. it was observed to grow luxuriously at ph 9 and tolerate highly alkaline environment up to ph 12. orange red color was a peculiar character of these cells which on purification obtained 60-80 mg/l and found to be sphingosine type of sulfonolipid "sulfobacin a" on the basis of infrared, nuclear magnetic res ... | 2009 | 19034697 |
| ind-6, a highly divergent ind-type metallo-beta-lactamase from chryseobacterium indologenes strain 597 isolated in burkina faso. | the genus chryseobacterium and other genera belonging to the family flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. several species of flavobacteriaceae, including chryseobacterium indologenes, are naturally resistant to beta-lactam antibiotics (including carbapenems), due to the production of a resident metallo-beta-lactamase. although c. indologenes presently constitutes a limited clinical threat, the incidence of inf ... | 2009 | 19651915 |
| chryseobacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede arthrosphaera magna attems. | a bacterial strain (cc-vm-7t), isolated from the faeces of the pill millipede arthrosphaera magna attems collected in india, was studied to determine its taxonomic allocation. cells stained gram-negative and were rod-shaped. comparative analyses of the 16s rrna gene sequence of the strain with those of the most closely related species clearly suggested allocation to the genus chryseobacterium, with the highest sequence similarities of 99.2% to chryseobacterium gleum ccug 14555t, 98.6% to chryseo ... | 2010 | 19749032 |
| cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of chryseobacterium gleum. | cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (add) by chryseobacterium gleum. spent lb media, containing cholesterol (3 mm approximately = 1 g l(-1)) where the bacterium was grown for 24 h, at 30 degrees c with constant shaking at 120 rpm, had the highest enzyme activity (167 u mg(-1)). the growing cells produced 0.076 g add from 1 g cholesterol l(-1). | 2010 | 20127502 |
| characterization of csp-1, a novel extended-spectrum beta-lactamase produced by a clinical isolate of capnocytophaga sputigena. | the capnocytophaga sputigena isolate nor, responsible for septicemia, was resistant to amoxicillin and narrow-spectrum cephalosporins. in a cloning experiment, a new gene, bla(csp-1), was identified; this gene encodes a novel extended-spectrum beta-lactamase (esbl) that shares only 52% and 49% identities with the cme-1 and veb-1 beta-lactamases, respectively. the g+c content of this gene, its genetic environment, the absence of conjugation transfer, and its detection in two reference strains sug ... | 2010 | 20308380 |
| achromobacter xylosoxidans respiratory tract infection in cystic fibrosis patients. | the aims of this study were to evaluate the frequency of achromobacter xylosoxidans infection in a cohort of cystic fibrosis patients, to investigate antimicrobial sensitivity, to establish possible clonal likeness among strains, and to address the clinical impact of this infection or colonization on the general outcome of these patients. the study was undertaken between january 2004 and december 2008 on 300 patients receiving care at the regional cystic fibrosis center of the naples university ... | 2011 | 21279730 |
| a novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and dna viral systems. | the use of nucleases as toxins for defense, offense or addiction of selfish elements is widely encountered across all life forms. using sensitive sequence profile analysis methods, we characterize a novel superfamily (the sukh superfamily) that unites a diverse group of proteins including smi1/knr4, pgs2, fbxo3, skip16, syd, herpesviral us22, irs1 and trs1, and their bacterial homologs. using contextual analysis we present evidence that the bacterial members of this superfamily are potential imm ... | 2011 | 21306995 |
| structural properties of 2/2 hemoglobins: the group iii protein from helicobacter hepaticus. | the e-proteobacterium helicobacter hepaticus (hh) contains a gene coding for a hemoglobin (hb). the protein belongs to the 2/2 hb lineage and is representative of group iii, a set of hbs about which little is known. an expression and purification procedure was developed for hh hb. electronic absorption and nuclear magnetic resonance (nmr) spectra were used to characterize ligation states of the ferric and ferrous protein. the pk(a) of the acid/alkaline transition of ferric hh hb was 7.3, an unus ... | 2011 | 21445851 |
| a xylanase gene directly cloned from the genomic dna of alkaline wastewater sludge showing application potential in the paper industry. | a xylanase gene, aws-2x, was directly cloned from the genomic dna of the alkaline wastewater sludge using degenerated pcr and modified tail-pcr. the deduced amino acid sequence of aws-2x shared the highest identity (60%) with the xylanase from chryseobacterium gleum belonging to the glycosyl hydrolase gh family 10. recombinant aws-2x was expressed in escherichia coli bl21 (de3) and purified to electrophoretic homogeneity. the enzyme showed maximal activity at ph 7.5 and 55 °c, maintained more th ... | 2011 | 21484273 |
| genome characterization of the polyvalent lytic bacteriophage gte2 with the potential for biocontrol of gordonia, rhodococcus and nocardia stabilized foams in activated sludge plants. | hydrophobic actinobacteria are commonly associated with the stabilization of foams in activated sludge systems. one possible attractive approach to control these foam stabilizing organisms is to use specific bacteriophages. we describe the genome characterization of a novel polyvalent dna phage, gte2, isolated from activated sludge. this phage is lytic for gordonia terrae, rhodococcus globerulus, rhodococcus erythropolis, rhodococcus erythropolis, nocardia otitididiscaviarum, and nocardia brasil ... | 2011 | 21498753 |
| comparative activities of tigecycline and other tetracyclines against non-fermenting gram-negative bacilli (excluding acinetobacter spp.). | tigecycline, a glycylcycline, is a semisynthetic derivative of minocycline with a broad spectrum of activity against aerobic and anaerobic bacteria (2, 12, 16).… | 2011 | 21606223 |
| widespread occurrence of secondary lipid biosynthesis potential in microbial lineages. | bacterial production of long-chain omega-3 polyunsaturated fatty acids (pufas), such as eicosapentaenoic acid (epa, 20:5n-3) and docosahexaenoic acid (dha, 22:6n-3), is constrained to a narrow subset of marine γ-proteobacteria. the genes responsible for de novo bacterial pufa biosynthesis, designated pfaeabcd, encode large, multi-domain protein complexes akin to type i iterative fatty acid and polyketide synthases, herein referred to as "pfa synthases". in addition to the archetypal pfa synthase ... | 2011 | 21629834 |
| provirus induction in hyperthermophilic archaea: characterization of aeropyrum pernix spindle-shaped virus 1, apsv1, and aeropyrum pernix ovoid virus 1, apov1. | by in silico analysis we have identified two putative proviruses in the genome of the hyperthermophilic archaeaon aeropyrum pernix and in special conditions of a. pernix growth we were able to induce their replication. both viruses were isolated and characterized. negatively stained virions of one virus appeared as pleomorphic spindle-shaped particles, 180-210 nm × 40-55 nm, with tails of heterogeneous length, in the range of 0-300 nm. this virus was named aeropyrum pernix spindle-shaped virus 1 ... | 2011 | 21784945 |
| emerging roles for the ro 60-kda autoantigen in noncoding rna metabolism. | all cells contain an enormous variety of ribonucleoprotein (rnp) complexes that function in diverse processes. although the mechanisms by which many of these rnps contribute to cell metabolism are well understood, the roles of others are only now beginning to be revealed. a member of this latter category, the ro 60-kda protein and its associated noncoding y rnas, was discovered because the protein component is a frequent target of the autoimmune response in patients with the rheumatic diseases s ... | 2011 | 21823229 |
| characterization of cia-1, an ambler class a extended-spectrum β-lactamase from chryseobacterium indologenes. | an ambler class a β-lactamase gene, bla(cia-1), was cloned from the reference strain chryseobacterium indologenes atcc 29897 and expressed in escherichia coli bl21. the bla(cia-1) gene encodes a novel extended-spectrum β-lactamase (esbl) that shared 68% and 60% identities with the cga-1 and cme-1 β-lactamases, respectively. bla(cia-1)-like genes were detected from clinical isolates. in addition to the metallo-β-lactamase ind of ambler class b, c. indologenes has a class a esbl gene, bla(cia-1), ... | 2012 | 22083470 |
| wide variation in antibiotic resistance proteins identified by functional metagenomic screening of a soil dna library. | most genes for antibiotic resistance present in soil microbes remain unexplored because most environmental microbes cannot be cultured. only recently has the identification of these genes become feasible through the use of culture-independent methods. we screened a soil metagenomic dna library in an escherichia coli host for genes that can confer resistance to kanamycin, gentamicin, rifampin, trimethoprim, chloramphenicol, or tetracycline. the screen revealed 41 genes that encode novel protein v ... | 2012 | 22247132 |
| a structure-based classification of class a β-lactamases, a broadly diverse family of enzymes. | for medical biologists, sequencing has become a commonplace technique to support diagnosis. rapid changes in this field have led to the generation of large amounts of data, which are not always correctly listed in databases. this is particularly true for data concerning class a β-lactamases, a group of key antibiotic resistance enzymes produced by bacteria. many genomes have been reported to contain putative β-lactamase genes, which can be compared with representative types. we analyzed several ... | 2016 | 26511485 |
| a structure-based classification of class a β-lactamases, a broadly diverse family of enzymes. | for medical biologists, sequencing has become a commonplace technique to support diagnosis. rapid changes in this field have led to the generation of large amounts of data, which are not always correctly listed in databases. this is particularly true for data concerning class a β-lactamases, a group of key antibiotic resistance enzymes produced by bacteria. many genomes have been reported to contain putative β-lactamase genes, which can be compared with representative types. we analyzed several ... | 2016 | 26511485 |
| bioinformatic characterization of the 4-toluene sulfonate uptake permease (tsup) family of transmembrane proteins. | the ubiquitous sequence diverse 4-toluene sulfonate uptake permease (tsup) family contains few characterized members and is believed to catalyze the transport of several sulfur-based compounds. prokaryotic members of the tsup family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. despite unequal representation, homologues from the three taxonomic domains of life share well-conserved motifs. ... | 2012 | 22192777 |
| bioinformatic characterization of the 4-toluene sulfonate uptake permease (tsup) family of transmembrane proteins. | the ubiquitous sequence diverse 4-toluene sulfonate uptake permease (tsup) family contains few characterized members and is believed to catalyze the transport of several sulfur-based compounds. prokaryotic members of the tsup family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. despite unequal representation, homologues from the three taxonomic domains of life share well-conserved motifs. ... | 2012 | 22192777 |
| gliding motility and por secretion system genes are widespread among members of the phylum bacteroidetes. | the phylum bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. recently, a novel protein secretion system, the por secretion system (porss), was identified in two members of the phylum, the gliding bacterium flavobacterium johnsoniae and the nonmotile oral pathogen porphyromonas gingivalis. the components of the porss are not similar in sequence to those of other well-studied bacterial secretion systems ... | 2013 | 23123910 |
| genome-based taxonomic classification of bacteroidetes. | the bacterial phylum bacteroidetes, characterized by a distinct gliding motility, occurs in a broad variety of ecosystems, habitats, life styles, and physiologies. accordingly, taxonomic classification of the phylum, based on a limited number of features, proved difficult and controversial in the past, for example, when decisions were based on unresolved phylogenetic trees of the 16s rrna gene sequence. here we use a large collection of type-strain genomes from bacteroidetes and closely related ... | 2016 | 28066339 |
| microbe-specific unconventional t cells induce human neutrophil differentiation into antigen cross-presenting cells. | the early immune response to microbes is dominated by the recruitment of neutrophils whose primary function is to clear invading pathogens. however, there is emerging evidence that neutrophils play additional effector and regulatory roles. the present study demonstrates that human neutrophils assume ag cross-presenting functions and suggests a plausible scenario for the local generation of apc-like neutrophils through the mobilization of unconventional t cells in response to microbial metabolite ... | 2014 | 25165152 |
| functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter. | targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ... | 2013 | 24062736 |
| streptophyte phytochromes exhibit an n-terminus of cyanobacterial origin and a c-terminus of proteobacterial origin. | phytochromes are red light-sensitive photoreceptors that control a variety of developmental processes in plants, algae, bacteria and fungi. prototypical phytochromes exhibit an n-terminal tridomain (pgp) consisting of pas, gaf and phy domains and a c-terminal histidine kinase (hk). | 2015 | 25886068 |
| performances of the vitek ms matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. | rapid and cost-effective matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms)-based systems will replace conventional phenotypic methods for routine identification of bacteria. we report here the first evaluation of the new maldi-tof ms-based vitek ms system in a large clinical microbiology laboratory. this system uses an original spectrum classifier algorithm and a specific database designed for the identification of clinically relevant species. we have te ... | 2012 | 22593596 |
| repertoire of free-living protozoa in contact lens solutions. | the repertoire of free-living protozoa in contact lens solutions is poorly known despite the fact that such protozoa may act as direct pathogens and may harbor intra-cellular pathogens. | 2016 | 27793130 |
| comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of bacillus anthracis spores in suspicious white powders and environmental samples. | we conducted a comprehensive, multiphase laboratory evaluation of the anthrax biothreat alert(®) test strip, a lateral flow immunoassay (lfa) for the rapid detection of bacillus anthracis spores. the study, conducted at 2 sites, evaluated this assay for the detection of spores from the ames and sterne strains of b. anthracis, as well as those from an additional 22 strains. phylogenetic near neighbors, environmental background organisms, white powders, and environmental samples were also tested. ... | 2016 | 27661796 |
| whole genome analysis of leptospira licerasiae provides insight into leptospiral evolution and pathogenicity. | the whole genome analysis of two strains of the first intermediately pathogenic leptospiral species to be sequenced (leptospira licerasiae strains var010 and mmd0835) provides insight into their pathogenic potential and deepens our understanding of leptospiral evolution. comparative analysis of eight leptospiral genomes shows the existence of a core leptospiral genome comprising 1547 genes and 452 conserved genes restricted to infectious species (including l. licerasiae) that are likely to be pa ... | 2012 | 23145189 |
| eukaryote to gut bacteria transfer of a glycoside hydrolase gene essential for starch breakdown in plants. | lateral gene transfer (lgt) between bacteria constitutes a strong force in prokaryote evolution, transforming the hierarchical tree of life into a network of relationships between species. in contrast, only a few cases of lgt from eukaryotes to prokaryotes have been reported so far. the distal animal intestine is predominantly a bacterial ecosystem, supplying the host with energy from dietary polysaccharides through carbohydrate-active enzymes absent from its genome. it has been suggested that l ... | 2012 | 22934241 |
| cultivation of denitrifying bacteria: optimization of isolation conditions and diversity study. | an evolutionary algorithm was applied to study the complex interactions between medium parameters and their effects on the isolation of denitrifying bacteria, both in number and in diversity. growth media with a ph of 7 and a nitrogen concentration of 3 mm, supplemented with 1 ml of vitamin solution but not with sodium chloride or riboflavin, were the most successful for the isolation of denitrifiers from activated sludge. the use of ethanol or succinate as a carbon source and a molar c/n ratio ... | 2006 | 16597968 |
| comparative activities of clinafloxacin against gram-positive and -negative bacteria. | activities of clinafloxacin, ciprofloxacin, levofloxacin, sparfloxacin, trovafloxacin, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, ceftazidime, and imipenem against 354 ciprofloxacin-susceptible and -intermediate-resistant organisms were tested by agar dilution. clinafloxacin yielded the lowest quinolone mics (< or = 0.5 microg/ml against ciprofloxacin-susceptible organisms and < or = 16.0 microg/ml against ciprofloxacin-intermediate-resistant organisms) compared to tho ... | 1998 | 9593165 |
| dna-dna hybridization study of strains of chryseobacterium, elizabethkingia and empedobacter and of other usually indole-producing non-fermenters of cdc groups iic, iie, iih and iii, mostly from human clinical sources, and proposals of chryseobacterium bernardetii sp. nov., chryseobacterium carnis sp. nov., chryseobacterium lactis sp. nov., chryseobacterium nakagawai sp. nov. and chryseobacterium taklimakanense comb. nov. | the taxonomic classification of 182 phenotypically similar isolates was evaluated using dna-dna hybridization and 16s rrna gene sequence analysis. these bacterial isolates were mainly derived from clinical sources; all were gram-negative non-fermenters and most were indole-producing. phenotypically, they resembled species from the genera chryseobacterium, elizabethkingia or empedobacter or belonged to cdc groups iic, iie, iih and iii. based on these analyses, four novel species are described: ch ... | 2013 | 23934253 |
| emerging flavobacterial infections in fish: a review. | flavobacterial diseases in fish are caused by multiple bacterial species within the family flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks around the world. in addition to directly imposing negative economic and ecological effects, flavobacterial disease outbreaks are also notoriously difficult to prevent and control despite nearly 100 years of scientific research. the emergence of recent reports linking previously uncharacterized flavobacteria to syst ... | 2014 | 26257926 |
| emerging flavobacterial infections in fish: a review. | flavobacterial diseases in fish are caused by multiple bacterial species within the family flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks around the world. in addition to directly imposing negative economic and ecological effects, flavobacterial disease outbreaks are also notoriously difficult to prevent and control despite nearly 100 years of scientific research. the emergence of recent reports linking previously uncharacterized flavobacteria to syst ... | 2014 | 26257926 |
| insights from the genome annotation of elizabethkingia anophelis from the malaria vector anopheles gambiae. | elizabethkingia anophelis is a dominant bacterial species in the gut ecosystem of the malaria vector mosquito anopheles gambiae. we recently sequenced the genomes of two strains of e. anophelis, r26t and ag1, isolated from different strains of a. gambiae. the two bacterial strains are identical with a few exceptions. phylogenetically, elizabethkingia is closer to chryseobacterium and riemerella than to flavobacterium. in line with other bacteroidetes known to utilize various polymers in their ec ... | 2014 | 24842809 |
| chryseobacterium indologenes in a woman with acute leukemia in senegal: a case report. | this report documents a rare case of chryseobacterium indologenes urinary tract infection in senegal. chryseobacterium indologenes is an uncommon human pathogen reported in hospital outbreaks in taiwan and there have been some sporadic cases reported in europe and in the usa mainly from immune-suppressed patients. | 2014 | 24886628 |
| noncontiguous finished genome sequence and description of weeksella massiliensis sp. nov. | strain ff8(t) (= csur p860 = dsm 28259) was isolated in dakar, senegal, from the urine of a 65-year-old man with acute cystitis. this strain shows a similarity of sequence of 16s rrna of 98.38% with weeksella virosa, and its genbank accession numbers are hg931340 and ccmh00000000. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. using a polyphasic study made of ph ... | 2015 | 26649182 |
| whole-genome sequence of chryseobacterium oranimense, a colistin-resistant bacterium isolated from a cystic fibrosis patient in france. | for the first time, we report the whole-genome sequence analysis of chryseobacterium oranimense g311, a multidrug-resistant bacterium, from a cystic fibrosis patient in france, including resistance to colistin. whole-genome sequencing of c. oranimense g311 was performed using ion torrent pgm, and rast, the embl-ebi server, and the antibiotic resistance gene-annotation (arg-annot) database were used for annotation of all genes, including antibiotic resistance (ar) genes. general features of the c ... | 2015 | 25583710 |
| evidence for elizabethkingia anophelis transmission from mother to infant, hong kong. | elizabethkingia anophelis, recently discovered from mosquito gut, is an emerging bacterium associated with neonatal meningitis and nosocomial outbreaks. however, its transmission route remains unknown. we use rapid genome sequencing to investigate 3 cases of e. anophelis sepsis involving 2 neonates who had meningitis and 1 neonate's mother who had chorioamnionitis. comparative genomics revealed evidence for perinatal vertical transmission from a mother to her neonate; the 2 isolates from these p ... | 2015 | 25625669 |
| a complete profile on blind-your-eye mangrove excoecaria agallocha l. (euphorbiaceae): ethnobotany, phytochemistry, and pharmacological aspects. | traditional system of medicine consists of large number of plants with various medicinal and pharmacological importances. this article provides a comprehensive review of the complete profile of an important mangrove plant excoecaria agallocha l. (euphorbiaceae) and elaborately describing the ethnobotany, phytochemistry, and pharmacological properties. it is used traditionally in the treatment of various diseases such as epilepsy, ulcers, leprosy, rheumatism, and paralysis. the latex obtained fro ... | 2016 | 28082796 |
| biochemical characterization of cps-1, a subclass b3 metallo-β-lactamase from a chryseobacterium piscium soil isolate. | cps-1 is a subclass b3 metallo-β-lactamase from a chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the gob-1 enzyme. cps-1 was overproduced in escherichia coli rosetta (de3), purified by chromatography, and biochemically characterized. this enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which overall resembles those of l1, gob-1, and acquired subclass b3 enzymes aim-1 and smb-1. | 2016 | 26666948 |
| aidc, a novel n-acylhomoserine lactonase from the potato root-associated cytophaga-flavobacteria-bacteroides (cfb) group bacterium chryseobacterium sp. strain strb126. | n-acylhomoserine lactones (ahls) are used as quorum-sensing (qs) signal molecules by many gram-negative bacteria. we have reported that chryseobacterium sp. strain strb126, which was isolated from the root surface of potato, has ahl-degrading activity. in this study, we cloned and characterized the aidc gene from the genomic library of strb126. aidc has ahl-degrading activity and shows homology to several metallo-β-lactamase proteins from bacteroidetes, although not to any known ahl-degrading en ... | 2012 | 22941089 |
| occurrence of horizontal gene transfer of p(ib)-type atpase genes among bacteria isolated from the uranium rich deposit of domiasiat in north east india. | uranium (u) tolerant aerobic heterotrophs were isolated from the subsurface soils of one of the pre-mined u-rich deposits at domiasiat located in the north-eastern part of india. on screening of genomic dna from 62 isolates exhibiting superior u and heavy metal tolerance, 32 isolates were found to be positive for p(ib)-type atpase genes. phylogenetic incongruence and anomalous dna base compositions revealed the acquisition of p(ib)-type atpase genes by six isolates through horizontal gene transf ... | 2012 | 23133569 |
| expression patterns reveal niche diversification in a marine microbial assemblage. | resolving the ecological niches of coexisting marine microbial taxa is challenging due to the high species richness of microbial communities and the apparent functional redundancy in bacterial genomes and metagenomes. here, we generated over 11 million illumina reads of protein-encoding transcripts collected from well-mixed southeastern us coastal waters to characterize gene expression patterns distinguishing the ecological roles of hundreds of microbial taxa sharing the same environment. the ta ... | 2012 | 22931830 |
| expression patterns reveal niche diversification in a marine microbial assemblage. | resolving the ecological niches of coexisting marine microbial taxa is challenging due to the high species richness of microbial communities and the apparent functional redundancy in bacterial genomes and metagenomes. here, we generated over 11 million illumina reads of protein-encoding transcripts collected from well-mixed southeastern us coastal waters to characterize gene expression patterns distinguishing the ecological roles of hundreds of microbial taxa sharing the same environment. the ta ... | 2012 | 22931830 |
| rapid discrimination of salmonella enterica serovar typhi from other serovars by maldi-tof mass spectrometry. | systemic infections caused by salmonella enterica are an ongoing public health problem especially in sub-saharan africa. essentially typhoid fever is associated with high mortality particularly because of the increasing prevalence of multidrug-resistant strains. thus, a rapid blood-culture based bacterial species diagnosis including an immediate sub-differentiation of the various serovars is mandatory. at present, maldi-tof based intact cell mass spectrometry (icms) advances to a widely used rou ... | 2012 | 22768195 |
| molecular docking simulation studies on potent butyrylcholinesterase inhibitors obtained from microbial transformation of dihydrotestosterone. | biotransformation is an effective technique for the synthesis of libraries of bioactive compounds. current study on microbial transformation of dihydrotestosterone (dht) (1) was carried out to produce various functionalized metabolites. | 2013 | 24103815 |
| transcriptome analysis of newt lens regeneration reveals distinct gradients in gene expression patterns. | regeneration of the lens in newts is quite a unique process. the lens is removed in its entirety and regeneration ensues from the pigment epithelial cells of the dorsal iris via transdifferentiation. the same type of cells from the ventral iris are not capable of regenerating a lens. it is, thus, expected that differences between dorsal and ventral iris during the process of regeneration might provide important clues pertaining to the mechanism of regeneration. in this paper, we employed next ge ... | 2013 | 23613853 |
| complete genome sequence of chryseobacterium sp. strain strb126, an n-acylhomoserine lactone-degrading bacterium isolated from potato root. | chryseobacterium sp. strain strb126 was isolated from a potato root and showed n-acylhomoserine lactone-degrading activity. here, we present the complete 5,503,743-bp genome sequence of strb126, which has a g+c content of 35.6% and carries 4,828 protein-coding genes, six rrna operons, and 80 trna genes. | 2014 | 25291777 |
| cloning, expression and biochemical characterization of the cholesterol oxidase cgchoa from chryseobacterium gleum. | cholesterol oxidases are important enzymes for applications such as the analysis of cholesterol in clinical samples, the synthesis of steroid derived drugs, and are considered as potential antibacterial drug targets. | 2014 | 24885249 |
| cloning, sequencing, and in silico analysis of β-propeller phytase bacillus licheniformis strain pb-13. | β -propeller phytases (bpphy) are widely distributed in nature and play a major role in phytate-phosphorus cycling. in the present study, a bpphy gene from bacillus licheniformis strain was expressed in e. coli with a phytase activity of 1.15 u/ml and specific activity of 0.92 u/mg proteins. the expressed enzyme represented a full length orf "phypb13" of 381 amino acid residues and differs by 3 residues from the closest similar existing bpphy sequences. the phypb13 sequence was characterized in ... | 2014 | 24864215 |
| molecular characterization of a novel n-acetyltransferase from chryseobacterium sp. | n-acetyltransferase from chryseobacterium sp. strain 5-3b is an acetyl coenzyme a (acetyl-coa)-dependent enzyme that catalyzes the enantioselective transfer of an acetyl group from acetyl-coa to the amino group of l-2-phenylglycine to produce (2s)-2-acetylamino-2-phenylacetic acid. we purified the enzyme from strain 5-3b and deduced the n-terminal amino acid sequence. the gene, designated nata, was cloned with two other hypothetical protein genes; the three genes probably form a 2.5-kb operon. t ... | 2014 | 24375143 |
| extracellular lipase and protease production from a model drinking water bacterial community is functionally robust to absence of individual members. | bacteria secrete enzymes into the extracellular space to hydrolyze macromolecules into constituents that can be imported for microbial nutrition. in bacterial communities, these enzymes and their resultant products can be modeled as community property. our goal was to investigate the impact of individual community member absence on the resulting community production of exoenzymes (extracellular enzymes) involved in lipid and protein hydrolysis. our model community contained nine bacteria isolate ... | 2015 | 26599415 |
| enhanced production of androst-1,4-diene-3,17-dione by mycobacterium neoaurum jc-12 using three-stage fermentation strategy. | to improve the androst-1,4-diene-3,17-dione (add) production from phytosterol by mycobacterium neoaurum jc-12, fructose was firstly found favorable as the initial carbon source to increase the biomass and eliminate the lag phase of m. neoaurum jc-12 in the phytosterol transformation process. based on this phenomenon, two-stage fermentation by using fructose as the initial carbon source and feeding glucose to maintain strain metabolism was designed. by applying this strategy, the fermentation dur ... | 2015 | 26352898 |
| discrepancy in maldi-tof ms identification of uncommon gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. | early identification of microbial organisms from respiratory secretions of patients with cystic fibrosis (cf) is important to guide therapeutic decisions. the objective was to compare the accuracy of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) relative to the conventional phenotypic method in identifying common bacterial isolates, including nonfermenting gram-negative bacteria, in a cohort of patients with cf. | 2015 | 25995646 |
| pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of tak1/mkk/p38. | the interrelationship between endogenous microbiota, the immune system, and tissue regeneration is an area of intense research due to its potential therapeutic applications. we investigated this relationship in schmidtea mediterranea, a model organism capable of regenerating any and all of its adult tissues. microbiome characterization revealed a high bacteroidetes to proteobacteria ratio in healthy animals. perturbations eliciting an expansion of proteobacteria coincided with ectopic lesions an ... | 2016 | 27441386 |
| chryseobacterium gleum pneumonia in an infant with nephrotic syndrome. | chryseobacterium gleum is commonly distributed in the environment. it can cause a wide variety of infections in immunocompromised patients in hospital setting. | 2016 | 27419074 |
| continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana fusarium wilt suppression. | fusarium wilt of banana always drives farmers to find new land for banana cultivation due to the comeback of the disease after a few cropping years. a novel idea for solving this problem is the continuous application of bioorganic fertilizer (bio), which should be practiced from the beginning of banana planting. in this study, bio was applied in newly reclaimed fields to pre-control banana fusarium wilt and the culturable rhizobacteria community were evaluated using biolog ecoplates and culture- ... | 2016 | 27306096 |
| [effects of continuous application of bio-organic fertilizer on banana production and cultural microflora of bulk soil in orchard with serious disease incidence]. | a field experiment was conducted for two years to investigate the effects of different fertilization applications on the suppression of banana fusarium wilt disease, crop yield, fruit quality and culturable microflora in a banana orchard which has been monocultured with banana for 12 years and suffered serious banana fusarium wilt disease. the fertilizers included chemical fertilizer (cf), cow manure compost (cm), pig manure compost (pm) and bio-organic fertilizer (bio). the banana soil microflo ... | 2015 | 26094464 |
| chryseobacterium cucumeris sp. nov., an endophyte isolated from cucumber (cucumis sativus l.) root and emended description of chryseobacterium arthrosphaerae. | the gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain gse06t, isolated from the surface-sterilized root of a cucumber plant grown in a field in gunsan, korea, was characterized by not only cultural and morphological features but also physiological, biochemical, and molecular analyses. phylogenetic analyses based on 16s rrna gene sequences revealed that strain gse06t was most related to species of the genus chryseobacterium. furthermore, strain gse06t exhibited the highest sequen ... | 2016 | 27902284 |
| chryseobacterium artocarpi sp. nov., isolated from the rhizosphere soil of artocarpus integer. | a bacterial strain, designated utm-3(t), isolated from the rhizosphere soil of artocarpus integer (cempedak) in malaysia was studied to determine its taxonomic position. cells were gram-stain-negative, non-spore-forming rods, devoid of flagella and gliding motility, that formed yellow-pigmented colonies on nutrient agar and contained mk-6 as the predominant menaquinone. comparative analysis of the 16s rrna gene sequence of strain utm-3(t) with those of the most closely related species showed tha ... | 2014 | 24958763 |
| identification, characterization, and biofilm formation of clinical chryseobacterium gleum isolates. | chryseobacterium gleum is not commonly isolated from clinical source(s). using 16s rrna gene sequencing, we identified 15 c. gleum isolates from the central region hospital alliance, taiwan, which were all misidentified: 14 as chryseobacterium indologenes and 1 as elizabethkingia meningoseptica using the vitek 2 gn card. matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a rapid and clinically applicable method, was evaluated for the identification of c. gleum, and the ... | 2014 | 24796989 |
| chryseobacterium viscerum sp. nov., isolated from diseased fish. | a taxonomic study was carried out on five gram-staining-negative, catalase- and oxidase-positive, rod-shaped bacteria isolated from the gills and livers of five diseased rainbow trout. the five novel isolates were designated strains 687b-08(t), 445-08, 452-08, 453b-08 and 967b-08. in phylogenetic analyses based on 16s rrna gene sequences, the five novel strains appeared almost identical (99.0-100 % sequence similarity) and to belong to the genus chryseobacterium. strain 687b-08(t) (the strain se ... | 2012 | 22268076 |
| infection profile in chronic granulomatous disease: a 23-year experience from a tertiary care center in north india. | chronic granulomatous disease (cgd) is an inherited phagocytic disorder characterized by recurrent infections with usually catalase-positive organisms. infections in cgd from developing countries are expected to be different from those in the western countries. we report the profile of infections in children diagnosed with cgd from a tertiary care center in north india. | 2017 | 28332028 |
| chryseobacterium ginsengisoli sp. nov., isolated from the rhizosphere of ginseng and emended description of chryseobacterium gleum. | strain dcy 63(t), isolated from the rhizosphere of ginseng, was a gram-reaction-negative, strictly aerobic, non-motile, catalase- and oxidase-positive rod. 16s rrna gene sequence analysis revealed that strain dcy 63(t) belonged to the genus chryseobacterium and was closely related to chryseobacterium indoltheticum lmg 4025(t) (98.1 %), chryseobacterium ginsenosidimutans thg 15(t) (98.0 %) and chryseobacterium scophthalmum lmg 13028(t) (97.1 %). the major polar lipids of strain dcy 63(t) were pho ... | 2013 | 23378110 |
| sepsis due to chryseobacterium gleum in a diabetic patient with copd - case report and mini review. | | 2017 | 28890506 |
| synergistic effect of chryseobacterium gleum sp. suk with acc deaminase activity in alleviation of salt stress and plant growth promotion in triticum aestivum l. | soil salinity is major abiotic stresses affecting morphological, biochemical and physiological processes of plant growth. chryseobacterium gleum sp. suk isolated from salt-stressed soil exhibited acc (1-aminocyclopropane-1-carboxylate) deaminase activity with iaa (indole acetic acid), siderophore, ammonia, hydrogen cyanide production, 2% salt tolerance and fungal cell wall degrading enzyme production (cellulase, protease). the isolate also showed a poultry feather degrading activity which is the ... | 2017 | 28560646 |
| chryseobacterium gleum in a man with prostatectomy in senegal: a case report and review of the literature. | here we report a rare case of a urinary tract infection due to chryseobacterium gleum. this widely distributed gram-negative bacillus is an uncommon human pathogen and is typically associated with health care settings. | 2017 | 28438192 |
| urinary tract infection due to chryseobacterium gleum, an uncommon pathogen. | chryseobacterium species are gaining importance as an emerging opportunistic nosocomial pathogen. limited availability of clinical data necessitates reporting of such isolates. we report a case of nosocomial urinary tract infection by metallo-β-lactamase-producing chryseobacterium gleum in an elderly diabetic male with chronic renal disease. identification and antibiotic sensitivity test performed by conventional methods were confirmed by matrix-assisted laser desorption ionization time-of-fligh ... | 2017 | 27721297 |
| pyonephrosis due to chryseobacterium gleum: a first case report. | chryseobacterium spp are widely distributed in nature but data of their isolation from clinical samples is scanty. here, we report the first case of ampc producing c. gleum causing pyonephrosis in a patient having bilateral nephrolithiasis on double j (dj) stent. the present isolate was resistant to vancomycin, erythromycin, clindamycin, carbapenems and ciprofloxacin and susceptible to tetracycline and minocycline. the patient was treated with tetracycline and recovered without the need for remo ... | 2015 | 25865994 |
| chryseobacterium gleum - a novel bacterium species detected in neonatal respiratory tract infections. | we report three patients with early neonatal infections. all patients had respiratory tract involvement with increased inflammation markers. chryseobacterium gleum was cultured from the stomach content aspirated on arrival at the neonatal intensive care unit and it was identified with the help of a microflex™ maldi biotyper mass spectrometer (bruker-daltonik, fremont, ca). recovery could be achieved with ciprofloxacin treatment. we consider our cases a possible new clinical presentation of a rar ... | 2014 | 24410052 |