| properties and biodegradation of a bioemulsifier from corynebacterium hydrocarboclastus. | an extracellular polymer was produced by continuous fermentation of corynebacterium hydrocarboclastus on kerosene in a 24 liter reactor. this polymer was composed of protein, lipid, and carbohydrates. the polymer possessed surface active properties, and had two critical micelle concentrations. its effectiveness was quite comparable to the effectiveness of synthetic surface active agents such as tween 80 and span 20; however, its efficiency was much lower. the polymer also had emulsifying propert ... | 1977 | 19111 |
| [infrared spectra of extreme and obligate thermophilic bacteria of the genus thermus]. | a recording double-beam spectrophotometer ur-10 was used to obtain ir spectra of the extreme thermophilic non-sporeforming bacterium thermus flavus, the obligate thermophilic non-sporeforming bacterium thermus ruber, the thermotolerant bacterium pseudomonas thermophilus, the sporeforming bacterium bacillus stearothermophilus, and the mesophilic bacteria pseudomonas aeruginosa, ps. denitrificans, flavobacterium breve, flavobact. arborescens. no significant differences have been found in the ir sp ... | 1975 | 814383 |
| acanthamoeba keratitis: synergy between amebic and bacterial cocontaminants in contact lens care systems as a prelude to infection. | we encountered a patient with acanthamoeba keratitis whose contact lens care solution contained numerous trophozoites and cysts admixed with xanthomonas maltophilia organisms, many of which were adherent to the trophozoite surface and internalized within endocytic vacuoles. because of this finding, we investigated the role of bacterial cocontaminants in contact lens care systems as substrates for the growth of acanthamoeba spp. individual cocultivation of acanthamoeba castellanii and a. polyphag ... | 1992 | 1401013 |
| 5s rrna sequences of representatives of the genera chlorobium, prosthecochloris, thermomicrobium, cytophaga, flavobacterium, flexibacter and saprospira and a discussion of the evolution of eubacteria in general. | 5s rrna sequences were determined for the green sulphur bacteria chlorobium limicola, chlorobium phaeobacteroides and prosthecochloris aestuarii, for thermomicrobium roseum, which is a relative of the green non-sulphur bacteria, and for cytophaga aquatilis, cytophaga heparina, cytophaga johnsonae, flavobacterium breve, flexibacter sp. and saprospira grandis, organisms allotted to the phylum 'bacteroides-cytophaga-flavobacterium' and relatives as determined by 16s rrna analyses. by using a cluste ... | 1990 | 1693658 |
| genotypic heterogeneity of flavobacterium group iib and flavobacterium breve demonstrated by dna-dna hybridization. | dna-dna hybridization studies on 42 stains presumptively identified as members of flavobacterium group iib and flavobacterium breve indicated pronounced genotypic heterogeneity within these taxa. three large groups highly related to the type strains of f. gleum, f. indologenes and f. breve respectively, and eight small groups were found. the group containing the type strain of f. breve was phenotypically indistinguishable from another genomic group, and these two groups were significantly separa ... | 1991 | 1892633 |
| chemical characterization of flavobacterium odoratum, flavobacterium breve, and flavobacterium-like groups iie, iih, and iif. | the cellular fatty acid, sphingolipid, and isoprenoid quinone compositions of flavobacterium odoratum, flavobacterium breve, and flavobacterium-like groups iie, iih, and iif were determined, using thin-layer, gas-liquid, and reverse-phase high-performance liquid chromatography. the fatty acid data showed that groups iie, iih, and iif were similar to recognized flavobacterium species by the presence of relatively large amounts of iso-branched hydroxy and nonhydroxy acids. groups iie and iih were ... | 1986 | 3700614 |
| satellite growth of legionella pneumophila with an environmental isolate of flavobacterium breve. | legionella pneumophila serogroup 1 was observed to satellite around colonies of flavobacterium breve on an l-cysteine-deficient medium which did not support growth of legionellae. both isolates were recovered from the hot water tanks of hospitals. ferric ppi stimulated satellite growth between 0.01 and 0.1%. | 1983 | 6660882 |
| flavobacterium breve meningitis in a dog. | an unusual, gram-negative rod was isolated in significant numbers (4+) from the cerebrospinal fluid (csf) of a dog. this isolate, flavobacterium breve, has not been identified previously as a pathogen in the dog. the case and the characteristics of the organism are described. | 1997 | 9358418 |
| characterization of marine temperate phage-host systems isolated from mamala bay, oahu, hawaii. | to understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. we isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained from concentrated water samples from mamala bay, oahu, hawaii. two of the rod-shaped bacterial hosts were identified as sphingomonas paucimobilis and flavobacterium sp. all of the phag ... | 1998 | 9464390 |
| processing respiratory specimens with c18-carboxypropylbetaine: development of a sediment resuspension buffer that contains lytic enzymes to reduce the contamination rate and lecithin to alleviate toxicity. | the c18-carboxypropylbetaine (cb-18) procedure for processing respiratory specimens for the detection of mycobacteria was shown to provide significant increases in sensitivity by smear and culture. however, the procedure also produced increased contamination, a loss in liquid culture sensitivity, and a reduction in smear specificity. because of these observations, the toxicity of cb-18 and the nature of the contamination were characterized. preincubation in 1 mm cb-18 impacted viability in a tim ... | 1998 | 9650952 |
| rearing stable fly larvae (diptera: muscidae) on an egg yolk medium. | the growth and survival of stomoxys calcitrans (l.) larvae on egg yolk medium inoculated with bacteria isolated from a colony of stable flies was evaluated. five species of bacteria--acinetobacter sp., aeromonas sp., empedobacter breve (holmes & owen), flavobacterium odoratum stutzer, and serratia marcescens bizio--were identified according to fatty acid profiles using a microbial identification system. larvae failed to develop on uninoculated plates, confirming that bacteria are required to com ... | 1999 | 10337112 |
| evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli. | the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ... | 2000 | 10699007 |
| microbiological investigation on black crusts from open-air stone monuments of bologna (italy). | samples of black crusts taken from open-air stone monuments in bologna dating back to between the twelfth and the nineteenth century were considered. the microbiological test procedures isolated from a high number of chemoorganotrophic bacteria, each of the samples although it was expected to find chemo-lithotrophic (specially sulfur-oxidizing and nitrifying) bacteria on account of the great amounts of sulphur, carbon, nitrogen dioxides and ammonia in the air. sporogenous and asporogenous specie ... | 2000 | 10872690 |
| a novel protein-deamidating enzyme from chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. | a novel protein-deamidating enzyme, which has potential for industrial applications, was purified from the culture supernatant of chryseobacterium proteolyticum strain 9670(t) isolated from rice field soil in tsukuba, japan. the deamidating activities on carboxybenzoxy (cbz)-gln-gly and caseins and protease activity were produced synchronously by the isolate. both deamidating activities were eluted as identical peaks separated from several proteases by phenyl-sepharose chromatography of the cult ... | 2000 | 10919788 |
| detection of legionella pneumophila using a real-time pcr hybridization assay. | a real-time pcr hybridization assay for legionella pneumophila is described; the assay uses lightcycler (idaho technology) methodology to specifically detect 2.5 cfu/reaction, equivalent to 1,000 cfu/liter of starting water sample. the assay, including dna extraction and confirmation of product identity, is completed within 90 min of receipt of a sample. | 2000 | 11060093 |
| outbreak of empedobacter brevis endophthalmitis after cataract extraction. | we present a series of patients who were operated upon on the same day by the same surgeon. all of those patients developed postoperative endophthalmitis due to empedobacter brevis - a bacterium hitherto unknown in ophthalmologic literature. | 2002 | 11981643 |
| ebr-1, a novel ambler subclass b1 beta-lactamase from empedobacter brevis. | empedobacter brevis (formerly designated flavobacterium breve) is a gram-negative aerobe involved in nosocomial infections. the ambler class b beta-lactamase gene bla(ebr-1) was cloned and expressed in escherichia coli from e. brevis clinical strain ass-1, which had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. purified beta-lactamase ebr-1 hydrolyzed penicillins, cephalosporins, and carbapenems efficiently but not aztreonam. kinetic parameters of ebr-1 were similar ... | 2002 | 12234848 |
| chromosome-encoded beta-lactamases tus-1 and mus-1 from myroides odoratus and myroides odoratimimus (formerly flavobacterium odoratum), new members of the lineage of molecular subclass b1 metalloenzymes. | myroides odoratus and myroides odoratimimus (formerly designated in a single species as flavobacterium odoratum) are gram-negative aerobes and sources of nosocomial infections in humans. they have variable susceptibility to beta-lactams and a decreased susceptibility to carbapenems. using genomic dnas of m. odoratus cip 103105 and m. odoratimimus cip 103073 reference strains, shotgun cloning of beta-lactamase genes was performed, followed by protein expression in escherichia coli. the deduced am ... | 2002 | 12384365 |
| evaluation of microscan autoscan for identification of pseudomonas aeruginosa isolates from cystic fibrosis patients. | accurate identification of gram-negative bacilli from cystic fibrosis (cf) patients is essential. only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as pseudomonas aeruginosa with microscan autoscan. most common misidentifications were pseudomonas fluorescens-pseudomonas putida (i.e., the strain was either p. fluorescens or p. putida, but the system did not make the distinction and yielded the result p. fluorescens/putida) and alcaligenes ... | 2003 | 12517904 |
| novel growth media for rearing larval horn flies, haematobia irritans (diptera: muscidae). | experiments were conducted to develop an agar-based medium for rearing immature horn flies, hematobia irritans (l.). larval survival was determined on sterilized manure inoculated with pure and mixed cultures of acinetobacter sp., bacillus pumilus meyer & gottheil, comamonas acidovorans den dooren de jong, pseudomonas mendocina palleroni, flavobacterium sp. and empedobacter breve (holmes & owen). rearing larvae on mixed cultures enhanced pupal weight but not survival. horn fly larvae failed to s ... | 2003 | 12597649 |
| evaluation of the vitek 2 id-gnb assay for identification of members of the family enterobacteriaceae and other nonenteric gram-negative bacilli and comparison with the vitek gni+ card. | we evaluated the vitek 2 id-gnb identification card (biomérieux, inc., durham, n.c.) for its ability to identify members of the family enterobacteriaceae and other gram-negative bacilli that are isolated in clinical microbiology laboratories. using 482 enteric stock cultures and 103 strains of oxidase-positive, gram-negative glucose-fermenting and nonfermenting bacilli that were maintained at -70 degrees c and passaged three times before use, we inoculated cards according to the manufacturer's d ... | 2003 | 12734254 |
| novel sulfonolipid in the extremely halophilic bacterium salinibacter ruber. | salinibacter ruber is an extremely halophilic bacterium, phylogenetically affiliated with the flavobacterium/cytophaga branch of the domain bacteria. electrospray mass analyses (negative ion) of the total lipid extract of a pure culture of s. ruber shows a characteristic peak at m/z 660 as the most prominent peak in the high-mass range of the spectrum. a novel sulfonolipid, giving rise to the molecular ion [m-h]- of m/z 660, has been identified. the sulfonolipid isolated and purified by thin-lay ... | 2004 | 15528534 |
| a novel and efficient enzymatic method for the production of peptides from unprotected starting materials. | we report herein the development of a novel and efficient enzymatic method for the production of oligopeptides. this newly discovered method is a simple, cost-effective process, using unprotected amino acids as substrates in an aqueous solution and producing peptides in high yield. the target of our initial screen was l-alanyl-l-glutamine, a dipeptide of significant industrial interest by virtue of its widespread use in infusion therapy. by means of the screening of microorganisms that can catal ... | 2005 | 15607239 |
| recognition of individual genes in diverse microorganisms by cycling primed in situ amplification. | cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ... | 2005 | 16269764 |
| impact of non-legionella bacteria on the uptake and intracellular replication of legionella pneumophila in acanthamoeba castellanii and naegleria lovaniensis. | in aquatic environments, legionella pneumophila survives, in association with other bacteria, within biofilms by multiplying in free-living amoebae. the precise mechanisms underlying several aspects of the uptake and intracellular replication of l. pneumophila in amoebae, especially in the presence of other bacteria, remain unknown. in the present study, we examined the competitive effect of selected non-legionella bacteria (escherichia coli, aeromonas hydrophila, flavobacterium breve, and pseud ... | 2005 | 16341636 |
| planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions. | legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ... | 2006 | 16597995 |
| inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken. | oxalic acid was evaluated as a treatment for reducing populations of naturally occurring microorganisms on raw chicken. raw chicken breasts were dipped in solutions of oxalic acid (0, 0.5, 1.0, 1.5, and 2.0%, wt/vol) for 10, 20, and 30 min, individually packed in oxygen-permeable polyethylene bags, and stored at 4 degrees c. total plate counts of aerobic bacteria and populations of pseudomonas spp. and enterobacteriaceae on breasts were determined before treatment and after storage for 1, 3, 7, ... | 2006 | 16924917 |
| description of wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera chryseobacterium and empedobacter. | a total of 26 isolates of non-fermenting, gram-negative rods, obtained between 1980 and 2004 by various clinical laboratories in belgium, with phenotypic characteristics resembling those of members of the genera chryseobacterium and empedobacter (indole-positive) and a biochemical profile resembling that of cdc group ii-h, but urease-positive, were collected at the université catholique de louvain microbiology laboratory, belgium. the 16s rrna gene sequences were determined for most of the isola ... | 2006 | 17012555 |
| replication of legionella pneumophila in biofilms of water distribution pipes. | biofilms similar to those present in water distribution pipes of anthropogenic aquatic systems were simulated in a rotating annular reactor using a non-legionella community consisting of aeromonas hydrophila, escherichia coli, flavobacterium breve and pseudomonas aeruginosa. the impact of this community and acanthamoeba castellanii on the replication of legionella pneumophila was investigated. despite the presence of 10(7) non-legionella bacteria, culture and real-time polymerase chain reaction ... | 2009 | 17644359 |
| replication of legionella pneumophila in floating biofilms. | biofilms are a major source of human pathogenic legionella pneumophila in aquatic systems. in this study, we investigated the capacity of l. pneumophila to colonize floating biofilms and the impact of acanthamoeba castellanii on the replication of biofilm-associated legionella. biofilms were grown in petri dishes and consisted of aeromonas hydrophila, escherichia coli, flavobacterium breve, and pseudomonas aeruginosa. six hours following inoculation, legionella were detected in floating biofilms ... | 2007 | 17713816 |
| host-plant selectivity of rhizobacteria in a crop/weed model system. | belowground microorganisms are known to influence plants' performance by altering the soil environment. plant pathogens such as cyanide-producing strains of the rhizobacterium pseudomonas may show strong host-plant selectivity. we analyzed interactions between different host plants and pseudomonas strains and tested if these can be linked to the cyanide sensitivity of host plants, the cyanide production of bacterial strains or the plant identity from which strains had been isolated. eight strain ... | 2007 | 17786217 |
| first isolation of two colistin-resistant emerging pathogens, brevundimonas diminuta and ochrobactrum anthropi, in a woman with cystic fibrosis: a case report. | cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections. | 2008 | 19061488 |
| ind-6, a highly divergent ind-type metallo-beta-lactamase from chryseobacterium indologenes strain 597 isolated in burkina faso. | the genus chryseobacterium and other genera belonging to the family flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. several species of flavobacteriaceae, including chryseobacterium indologenes, are naturally resistant to beta-lactam antibiotics (including carbapenems), due to the production of a resident metallo-beta-lactamase. although c. indologenes presently constitutes a limited clinical threat, the incidence of inf ... | 2009 | 19651915 |
| spectrum of activity, mutation rates, synergistic interactions, and the effects of ph and serum proteins for fusidic acid (cem-102). | fusidic acid (cem-102) is a steroidal antimicrobial agent with focused gram-positive activity that acts by preventing bacterial protein synthesis via interacting with elongation factor g. a collection of 114 wild-type isolates (> 80 species) was used to define the contemporary limits of fusidic acid spectrum against gram-positive and gram-negative species. reference broth microdilution and anaerobic agar dilution methods were performed. modifications of standardized test methods included adding ... | 2010 | 20159376 |
| depsides and other polar constituents from origanum dictamnus l. and their in vitro antimicrobial activity in clinical strains. | phytochemical investigation of the polar extracts of the aerial parts of origanum dictamnus afforded 15 secondary metabolites. one new depside was isolated, to which the trivial name salvianolic acid p (1) was given, in addition to the known depsides rosmarinic acid (2) and rosmarinic acid methyl ester (3), as well as two monoterpenes, thymoquinone (4) and thymoquinol 2-o-beta-glucopyranoside (5); two simple phenolic acids, oresbiusin a (6) and e-caffeic acid (7); six flavonoids, namely, apigeni ... | 2010 | 20423097 |
| [a case of anaphylactoid purpura suggested to empedobacter (flavobacterium) brevis infection concerned]. | the patient was an 83 year-old woman with purpura on her right foot and on both crura starting from the end of november 2005. first, she took medical advice from a nearby doctor, but it did not improve. since blisters were formed besides purpura, she visited nagoya city midori municipal hospital. when she visited, she had many purpura on her both legs, her right lower crus and her right foot was swelling with redness, blisters and erosion. histopathological finding showed leukocytoclastic vascul ... | 2010 | 20502105 |
| interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms. | it is well established that legionella pneumophila is a waterborne pathogen; by contrast, the mode of helicobacter pylori transmission remains unknown but water seems to play an important role. this work aims to study the influence of five microorganisms isolated from drinking water biofilms on the survival and integration of both of these pathogens into biofilms. | 2011 | 21418578 |
| complete genome sequence of weeksella virosa type strain (9751). | weeksella virosa holmes et al. 1987 is the sole member and type species of the genus weeksella which belongs to the family flavobacteriaceae of the phylum bacteroidetes. twenty-nine isolates, collected from clinical specimens provided the basis for the taxon description. while the species seems to be a saprophyte of the mucous membranes of healthy man and warm-blooded animals a causal relationship with disease has been reported in a few instances. except for the ability to produce indole and to ... | 2011 | 21475590 |
| factors influencing the microbial composition of metalworking fluids and potential implications for machine operator's lung. | hypersensitivity pneumonitis, also known as "machine operator's lung" (mol), has been related to microorganisms growing in metalworking fluids (mwfs), especially mycobacterium immunogenum. we aimed to (i) describe the microbiological contamination of mwfs and (ii) look for chemical, physical, and environmental parameters associated with variations in microbiological profiles. we microbiologically analyzed 180 mwf samples from nonautomotive plants (e.g., screw-machining or metal-cutting plants) i ... | 2012 | 22057869 |
| Gene cloning and characterization of a-amino acid ester acyl transferase in Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458. | The gene encoding a-amino acid ester acyl transferase (AET), the enzyme that catalyzes the peptide-forming reaction from amino acid methyl esters and amino acids, was cloned from Empedobacter brevis ATCC14234 and Sphingobacterium siyangensis AJ2458 and expressed in Escherichia coli. This is the first report on the aet gene. It encodes a polypeptide composed of 616 (ATCC14234) and 619 (AJ2458) amino acids residues. The V(max) values of these recombinant enzymes during the catalysis of L-alanyl-L- ... | 2011 | 22056425 |
| co-existence of legionella and other gram-negative bacteria in potable water from various rural and urban sources. | | 2011 | 22216808 |
| microbiology of the gut of the kola nut weevil, balanogastris kolae. | reports have shown that many insects have microbes in their gut system. gut microbes are very important for insect vitality and much of their nutrition is derived from products of microbial metabolism. the habitat of balanogastris kolae (desbrocher des loges) (coleoptera: curculionidae) suggests that they possess the ability to digest varieties of sugars particularly starch and protein materials present in the kola nut, cola nitida schott & endlicher (malvales: malvaceae). the aim of this study ... | 2012 | 23421598 |
| molecular detection of bacteria in plant tissues, using universal 16s ribosomal dna degenerated primers. | highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. we report here the development of a sensitive polymerase chain reaction (pcr) based method for the rapid detection and identification of bacteria occurring in plant tissue cul ... | 2014 | 26019546 |
| genome-based taxonomic classification of bacteroidetes. | the bacterial phylum bacteroidetes, characterized by a distinct gliding motility, occurs in a broad variety of ecosystems, habitats, life styles, and physiologies. accordingly, taxonomic classification of the phylum, based on a limited number of features, proved difficult and controversial in the past, for example, when decisions were based on unresolved phylogenetic trees of the 16s rrna gene sequence. here we use a large collection of type-strain genomes from bacteroidetes and closely related ... | 2016 | 28066339 |
| ability of the microscan rapid gram-negative id type 3 panel to identify nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. | the microscan rapid neg id3 panel is designed for the identification of enterobacteriaceae and nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. we evaluated this panel for its ability to identify gram-negative non-enterobacteriaceae bacteria. a total of 134 strains, representing 26 genera and 42 species, were taken from storage at -70(o)c, passaged three times before testing, and inoculated into the panels according to the manufacturer's directions before being inserted int ... | 2002 | 12354875 |
| non-pathogenic microflora of a spring water with regenerative properties. | the comano spring water (comano, italy) has been demonstrated to improve skin regeneration, not only by increasing keratinocyte proliferation and migration, but also by modulating the regenerated collagen and elastic fibers in the dermis. however, such biological properties may not be entirely explained by its mineral composition only. as the non-pathogenic bacterial populations have demonstrated an active role in different biological processes, the potential presence of non-pathogenic bacterial ... | 2015 | 26623012 |
| biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. | six hundred nineteen strains of nonfermenting gram-negative rods were tested for alkaline phosphatase, benzyl-arginine arylamidase, pyrrolidonyl arylamidase, ethylene glycol acidification, and susceptibility to desferrioxamine and colistin. the results were highly discriminant. therefore, the proposed tests may be helpful for the identification of this group of organisms. | 2002 | 11880447 |
| quantitative determination of free-dna uptake in river bacteria at the single-cell level by in situ rolling-circle amplification. | detection of plasmid dna uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (rca). uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in osaka, japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell permeabilization conditions. plasmid uptake determined by in situ rca was compared to direct counts of cells expressing gfp under fluorescence microsc ... | 2006 | 16957252 |
| dna-dna hybridization study of strains of chryseobacterium, elizabethkingia and empedobacter and of other usually indole-producing non-fermenters of cdc groups iic, iie, iih and iii, mostly from human clinical sources, and proposals of chryseobacterium bernardetii sp. nov., chryseobacterium carnis sp. nov., chryseobacterium lactis sp. nov., chryseobacterium nakagawai sp. nov. and chryseobacterium taklimakanense comb. nov. | the taxonomic classification of 182 phenotypically similar isolates was evaluated using dna-dna hybridization and 16s rrna gene sequence analysis. these bacterial isolates were mainly derived from clinical sources; all were gram-negative non-fermenters and most were indole-producing. phenotypically, they resembled species from the genera chryseobacterium, elizabethkingia or empedobacter or belonged to cdc groups iic, iie, iih and iii. based on these analyses, four novel species are described: ch ... | 2013 | 23934253 |
| emerging flavobacterial infections in fish: a review. | flavobacterial diseases in fish are caused by multiple bacterial species within the family flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks around the world. in addition to directly imposing negative economic and ecological effects, flavobacterial disease outbreaks are also notoriously difficult to prevent and control despite nearly 100 years of scientific research. the emergence of recent reports linking previously uncharacterized flavobacteria to syst ... | 2014 | 26257926 |
| emerging flavobacterial infections in fish: a review. | flavobacterial diseases in fish are caused by multiple bacterial species within the family flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks around the world. in addition to directly imposing negative economic and ecological effects, flavobacterial disease outbreaks are also notoriously difficult to prevent and control despite nearly 100 years of scientific research. the emergence of recent reports linking previously uncharacterized flavobacteria to syst ... | 2014 | 26257926 |
| noncontiguous finished genome sequence and description of weeksella massiliensis sp. nov. | strain ff8(t) (= csur p860 = dsm 28259) was isolated in dakar, senegal, from the urine of a 65-year-old man with acute cystitis. this strain shows a similarity of sequence of 16s rrna of 98.38% with weeksella virosa, and its genbank accession numbers are hg931340 and ccmh00000000. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. using a polyphasic study made of ph ... | 2015 | 26649182 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| taxonogenomics and description of vaginella massiliensis gen. nov., sp. nov., strain marseille p2517(t), a new bacterial genus isolated from the human vagina. | an obligate aerobic, gram-negative, nonmotile and nonsporulating rod designated marseille p2517 was isolated from the vaginal flora. we describe its features, annotate the genome and compare it to the closest species. the 16s rrna analysis shows 93.03% sequence similarity with weeksella virosa, the phylogenetically closest species. its genome is 2 434 475 bp long and presents 38.16% g+c. on the basis of these data, it can be considered as a new genus in the flavobacteriaceae family, for which we ... | 2016 | 28053707 |
| evaluation of the wider system, a new computer-assisted image-processing device for bacterial identification and susceptibility testing. | the wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. it has been adapted to be able to read and interpret commercial microscan panels. two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family enterobacteriaceae, 25 nonfermentative gram-negative rods [nfgnrs], and 81 gram-positive cocci) were tested. in addition, 100 enterobacterial strains with known beta-lactam re ... | 2000 | 10747104 |
| in vitro activities of ertapenem (mk-0826) against clinical bacterial isolates from 11 north american medical centers. | this study compared the in vitro activities of the new long-half-life carbapenem ertapenem (also known as mk-0826 and l-749,345) with those of imipenem, amoxicillin-clavulanate, and ciprofloxacin against 5,558 recent clinical isolates from 11 north american medical centers. we confirmed the greater activity of ertapenem than of imipenem against the enterobacteriaceae and the greater activity of imipenem against pseudomonads and gram-positive bacteria. | 2001 | 11353653 |
| legionella pneumophila persists within biofilms formed by klebsiella pneumoniae, flavobacterium sp., and pseudomonas fluorescens under dynamic flow conditions. | legionella pneumophila, the agent of legionnaires' disease pneumonia, is transmitted to humans following the inhalation of contaminated water droplets. in aquatic systems, l. pneumophila survives much of time within multi-organismal biofilms. therefore, we examined the ability of l. pneumophila (clinical isolate 130 b) to persist within biofilms formed by various types of aquatic bacteria, using a bioreactor with flow, steel surfaces, and low-nutrient conditions. l. pneumophila was able to inter ... | 2012 | 23185637 |
| an interesting case of empedobacter brevis bacteremia after right knee cellulitis. | | 2012 | 22754253 |
| ultrasound assessment of thrombotic complications in pediatric patients with tunneled central venous catheters. | central venous access consists in inserting a vascular catheter to the vena cava and placing its tip in the vicinity of the opening to the right atrium. in the patients of the clinic of pediatrics, hematology and oncology at the academic clinical centre of the medical university in gdańsk, such implantation procedures are conducted 40-50 times in a year using broviac/hickman catheters that are placed in the subclavian vein. in the ultrasound and biopsy laboratory at the clinic mentioned above, a ... | 2013 | 26673005 |
| emerging organisms in a tertiary healthcare set up. | one-tenth of all infectious diseases are attributable to emerging organisms. as emerging organisms sporadically affect a relatively small percentage of population they are not studied at large. this study was aimed at studying the characteristics of emerging organisms encountered from various clinical samples in an apex tertiary care multispeciality teaching and research hospital. | 2013 | 24843199 |
| emerging organisms in a tertiary healthcare set up. | one-tenth of all infectious diseases are attributable to emerging organisms. as emerging organisms sporadically affect a relatively small percentage of population they are not studied at large. this study was aimed at studying the characteristics of emerging organisms encountered from various clinical samples in an apex tertiary care multispeciality teaching and research hospital. | 2013 | 24843199 |
| intra-amoeba multiplication induces chemotaxis and biofilm colonization and formation for legionella. | legionella pneumophila, a facultative intracellular bacterium, is the causative agent of legionellosis. in the environment this pathogenic bacterium colonizes the biofilms as well as amoebae, which provide a rich environment for the replication of legionella. when seeded on pre-formed biofilms, l. pneumophila was able to establish and survive and was only found at the surface of the biofilms. different phenotypes were observed when the l. pneumophila, used to implement pre-formed biofilms or to ... | 2013 | 24205008 |
| pathogenic conjunctival bacteria associated with systemic co-morbidities of patients undergoing cataract surgery. | to identify the risk of patients undergoing cataract surgery of having pathogenic conjunctival bacteria associated with their systemic co-morbidities. | 2013 | 23703631 |
| sanitization of an automatic reverse-osmosis watering system: removal of a clinically significant biofilm. | during environmental monitoring of our institution's rodent watering systems, one vivarium was found to have high bacterial loads in the reverse-osmosis (ro) automatic water system. these findings prompted evaluation of the entire ro water production and distribution system. investigation revealed insufficient rack and ro system sanitization, leading to heavy biofilm accumulation within the system. approximately 2 wk after discovery in the water system, one of the bacterial organisms isolated in ... | 2013 | 23562105 |
| comparative evaluation of the in-vitro activity of six β-lactam/β-lactamase inhibitor combinations against gram negative bacilli. | the extensive use of the β-lactam antibiotics in hospitals and in the community has created major resistance problems which has led to increased morbidity, mortality and healthcare costs. the use of the β-lactamase inhibitors in combination with the β-lactam antibiotics is currently the most successful strategy used for circumventing the resistance mechanisms. | 2013 | 23543071 |
| trichosporon mycotoxinivorans infection in patients with cystic fibrosis. | we describe the clinical course of four patients who had trichosporon mycotoxinivorans recovered from multiple sputum cultures over time with various clinical consequences but no fatalities. we also report successful rapid identification of this organism using matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. | 2014 | 24648553 |
| exploring the cause of initially reactive bovine brains on rapid tests for bse. | bovine spongiform encephalopathy (bse) is an invariably fatal prion disease of cattle. the identification of the zoonotic potential of bse prompted safety officials to initiate surveillance testing for this disease. in canada, bse surveillance is primarily focused on high risk cattle including animals which are dead, down and unable to rise, diseased or distressed. this targeted surveillance results in the submission of brain samples with a wide range of tissue autolysis and associated contamina ... | 2015 | 26689488 |
| practically saline. | introduction. in december 2014, the food and drug administration issued a recall of all wallcur simulation products due to reports of their use in clinical practice. we present a case of septic shock and multiorgan failure after the accidental intravenous infusion of a nonsterile wallcur simulation product. case. the patient presented with symptoms of rigors and dyspnea occurring immediately after infusion of wallcur practi-0.9% saline. initial laboratory evidence was consistent with severe sept ... | 2015 | 26668812 |
| empedobacter brevis bacteremia in a patient infected with hiv: case report and review of literature. | clinical disease caused by empedobacter brevis (e. brevis) is very rare. we report the first case of e. brevis bacteremia in a patient with hiv and review the current literature. a 69-year-old man with human immunodeficiency virus (hiv) and cd4 count of 319 presented with chief complaints of black tarry stools, nausea and vomiting for 2 days. physical exam was significant for abdominal pain on palpation with no rebound or guarding. his total leukocyte count was 32,000 cells/μl with 82% neutrophi ... | 2015 | 26550499 |
| occurrence and control of legionella in recycled water systems. | legionella pneumophila is on the united states environmental protection agency (usepa) candidate contaminant list (ccl) as an important pathogen. it is commonly encountered in recycled water and is typically associated with amoeba, notably naegleria fowleri (also on the ccl) and acanthamoeba sp. no legionellosis outbreak has been linked to recycled water and it is important for the industry to proactively keep things that way. a review was conducted examine the occurrence of legionella and its p ... | 2015 | 26140674 |
| bacterial diversity associated with wild caught anopheles mosquitoes from dak nong province, vietnam using culture and dna fingerprint. | microbiota of anopheles midgut can modulate vector immunity and block plasmodium development. investigation on the bacterial biodiversity in anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of africa. vietnam is an endemic country for both malaria and bancroftian filariasis whose parasitic agents can be transmitted by the same anopheles species. no information on the microbio ... | 2015 | 25747513 |
| armadillidin h, a glycine-rich peptide from the terrestrial crustacean armadillidium vulgare, displays an unexpected wide antimicrobial spectrum with membranolytic activity. | antimicrobial peptides (amps) are key components of innate immunity and are widespread in nature, from bacteria to vertebrate animals. in crustaceans, there are currently 15 distinct amp families published so far in the literature, mainly isolated from members of the decapoda order. up to now, armadillidin is the sole non-decapod amp isolated from the haemocytes of armadillidium vulgare, a crustacean isopod. its first description demonstrated that armadillidin is a linear glycine-rich (47%) cati ... | 2016 | 27713732 |
| empedobacter brevis meningitis in a neonate: a very rare case of neonatal meningitis and literature review. | empedobacter brevis is gram-negative bacilli that belongs to flavobacteriaceae family. it was previously known with name of flavobacterium breve. the reservoir of these bacteria is soil, plants, water, food, hospital water sources, including incubators, sinks, faucets, tap water, hemodialysis systems, saline solutions, and other pharmaceutical solutions. we report a case of term female newborn, admitted with complaint of respiratory distress developing soon after birth and developed clinical fea ... | 2016 | 27648334 |
| variation of bacterial community diversity in rhizosphere soil of sole-cropped versus intercropped wheat field after harvest. | as the major crops in north china, spring crops are usually planted from april through may every spring and harvested in fall. wheat is also a very common crop traditionally planted in fall or spring and harvested in summer year by year. this continuous cropping system exhibited the disadvantages of reducing the fertility of soil through decreasing microbial diversity. thus, management of microbial diversity in the rhizosphere plays a vital role in sustainable crop production. in this study, ten ... | 2016 | 26934044 |
| chloramphenicol - a potent armament against multi-drug resistant (mdr) gram negative bacilli? | multidrug-resistant gram-negative bacteria cause infections which are hard to treat and cause high morbidity and mortality. due to limited therapeutic options there is a renewed interest upon older antimicrobials which had fallen into disuse as a result of toxic side effects. one such antibiotic is chloramphenicol which was sidelined due to reports linking its use with the development of aplastic anaemia. | 2016 | 27042458 |
| fatal burkholderia gladioli infection misidentified as empedobacter brevis in a lung transplant recipient with cystic fibrosis. | data describing the risk of lung transplantation (lt), clinical features, and outcomes of patients with cystic fibrosis (cf) infected with burkholderia gladioli are limited. herein, we report a case of disseminated b. gladioli infection characterized by bacteremia, necrotizing pneumonia, lung abscess, and empyema in a lung transplant recipient with cf, highlight the importance of accurate microbiological identification, and review published outcomes of lt in cf patients infected with b. gladioli ... | 2012 | 22429703 |
| algoriella xinjiangensis gen. nov., sp. nov., a new psychrotolerant bacterium of the family flavobacteriaceae. | an aerobic, gram-stain negative, non-spore-forming and psychrotolerant bacterium, designated strain xj109(t), was isolated from a sewage water sample collected from xinjiang uigur autonomous region, china. phylogenetic analysis based on 16s rrna gene sequence indicated that strain xj109(t) represents a novel member of the family flavobacteriaceae. the strain showed 95.5 % similarity with the 16s rrna gene sequence of empedobacter brevis lmg 4011(t), 95.4% with chishuiella changwenlii by4(t), 95. ... | 2015 | 26310805 |
| empedobacter stercoris sp. nov., isolated from an input sample of a biogas plant. | two gram-stain-negative, rod-shaped bacteria, strains 990b6_12er2at and 994b6_12er2a, were isolated during microbiological analysis of a mixed manure sample which was used as input material for a german biogas plant. phylogenetic identification based on nearly full-length 16s rrna gene sequences placed the isolates into the family flavobacteriaceae within the phylum bacteroidetes. strains 990b6_12er2at and 994b6_12er2a shared identical 16s rrna gene sequences and showed highest 16s rrna gene seq ... | 2015 | 26228269 |
| moheibacter sediminis gen. nov., sp. nov., a member of the family flavobacteriaceae isolated from sediment, and emended descriptions of empedobacter brevis, wautersiella falsenii and weeksella virosa. | a gram-reaction-negative, yellow-pigmented, strictly aerobic bacterium, designated m0116t, was isolated from the sediment of the mohe basin in north-east china. flexirubin-type pigments were produced. cells were catalase- and oxidase-positive and non-gliding rods. phylogenetic analysis based on 16s rrna gene sequences revealed that strain m0116t was a member of the family flavobacteriaceae and was most closely related to members of the genera empedobacter, wautersiella and weeksella with 90.5-91 ... | 2014 | 24453231 |
| moheibacter stercoris sp. nov isolated from an input sample of a german biogas plant. | a gram-negative rod-shaped bacterium, strain 784b1_12e-casot, was isolated from a mixed manure sample used as input material of a german biogas plant. phylogenetic identification based on the nearly full-length 16s rrna gene sequence placed the strain into the family flavobacteriaceae (bacteriodetes) with highest sequence similarity to the type strain of moheibacter sediminis (93.7%) followed by empedobacter brevis (90.7%). major cellular fatty acids of strain 784b1_12e-casot were iso-c15:0 and ... | 2016 | 27088161 |