[infection caused by flavobacterium meningosepticum, with special reference to purulent meningitis in newborn infants]. 1977405436
[flavobacterium meningosepticum (author's transl)]. 1977559147
flavobacterium meningosepticum as an opportunist.flavobacterium meningosepticum was isolated from the cerebrospinal fluid of an adult immunodeficient female. in spite of prompt therapy the patient succumbed to the infection. the opportunistic role of the organism is discussed.1978641195
[pyoventriculitis caused by flavobacterium meningosepticum; treatment with rifamycine]. 1978693224
[flavobacterium meningosepticum isolated from the oropharynx of a patient with acute myelogenous leukemia]. 1976828197
synthesis and inhibitory activity of acyl-peptidyl-pyrrolidine derivatives toward post-proline cleaving enzyme; a study of subsite specificity.several pyrrolidine derivatives have been synthesized and examined for their inhibitory activity on post-proline cleaving enzymes from flavobacterium meningosepticum and bovine brain. almost all the compounds tested in this study inhibited the activity of both enzymes at low ic50 values (from nm to microm) but a specificity difference was observed with alkylacyl-peptidyl-pyrrolidine derivatives which strongly inhibited only the bacterial enzyme. the most effective inhibitors have a proline resid ...19911669438
flavobacterium meningosepticum in the hospital environment.ten strains of flavobacterium meningosepticum were isolated from routine clinical material during a period of two years. of a number of antibiotics tested, clindamycin was the only one to which they were fully sensitive. the organism was found in chlorhexidine gluconate (hibitane) solutions in use on the wards for the storage of thermometers and for routine disinfection.1976977783
rifamycin in neonatal flavobacteria meningitis.three newborn infants with meningitis due to flavobacterium meningosepticum were treated with rifamycin administered parenterally and directly into the cerebral ventricles. antibiotic concentrations of blood and cerebrospinal fluid (csf) were monitored during treatment. there was rapid sterilization of the csf after this antibiotic. jaundice was the only toxicity noted. all 3 infants developed hydrocephalus and are shunt dependent. two of them are otherwise free of neurological complications and ...1976952554
two outbreaks of flavobacterium meningosepticum type e in a neonatal intensive care unit.two separate outbreaks due to flavobacterium meningosepticum type e occurred in a neonatal intensive care unit in march-april and july 1975. the first outbreak involved all five infants in the unit. two infants developed meningitis, one had bacteremia, and two were colonized. during the second outbreak, five of seven infants were colonized but none developed disease. the upper respiratory tract was colonized first in most instances, and the organism persisted at this site for a mean of 17.3 days ...1977925147
cellular fatty acids of flavobacterium meningosepticum and flavobacterium species group iib.the cellular fatty acid profiles of flavobacterium meningosepticum and flavobacterium species group iib were markedly different from those of related bacteria. the profiles were characterized by the presence of 13-methyl-tetradecanoate and three uncommon acids: 2-hydroxy-13-methyl-tetradecanoate, 15-methyl-hexadecanoate, and 3-hydroxy-15-methyl-hexadecanoate.1978744804
susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to newer antimicrobials.the susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to a variety of newer antimicrobials was determined. sixty-three amikacin-resistant isolates were collected from 61 inpatients at a tertiary-care hospital between 1985 and 1988. the organisms included xanthomonas maltophilia (24 isolates), pseudomonas aeruginosa (16), pseudomonas species (3), serratia marcescens (10), flavobacterium meningosepticum (4), enterobacter species (3), acinetobacter calcoaceticu ...19911860303
[flavobacterium meningosepticum as the cause of meningitis in a premature infant]. 1977611436
[six new serotypes of "flavobacterium meningosepticum" (author's transl)].six new serotypes (g to l) of flavobacterium meningosepticum are described herein. cross-reactions between the 12 serotypes, a to l (including the 6 serotypes a to f of owen and lapage, 1974) are described. sixty-six strains isolated in france (61 from strasbourg) belong to the following serotypes (with number of strains): a (1), f (4), g (51), h (1), i (1), j (6), k (1) and l (1). use of meningosepticum serotyping is recommended as an epidemiological tool.1979507635
flavobacterium meningosepticum infections. 1979451641
[clinical observation of neonatal meningitis caused by flavobacterium meningosepticum].from january 1981 to december 1988, we collected 11 cases of neonatal meningitis caused by flavobacterium meningosepticum. the 6 male and 5 female newborns ranged from 3 days to 20 days old. birth body weight varied from 1100 gm to 3600 gm. seven cases were premature or small for date. nosocomial infection was noted in 7 of these 11 cases. clinically, lethargy and poor activity were the most common symptoms. cyanosis, fever and convulsion were the next. there were 9 cases showing pleocytosis, in ...19911776441
multiple endoglycosidase (endo) f activities expressed by flavobacterium meningosepticum. endo f1: molecular cloning, primary sequence, and structural relationship to endo h.a full-length insert for the endo-beta-n-acetylglucosaminidase (endo) f1 gene was located on a 2,200-base pair ecori fragment of genomic dna and cloned into the plasmid vector bluescript. transformed escherichia coli cells expressed endo f1 activity very well, but the enzyme apparently was not processed and secreted into the medium as it normally is in flavobacterium meningosepticum. dna sequencing revealed an open reading frame of 1,017 nucleotides encoding a putative 50-amino acid signal seque ...19921740434
in vitro antibacterial activity of me1207, a new oral cephalosporin.me1207 is the prodrug of me1206. its in vitro antibacterial activity was compared with that of cefteram, cefpodoxime, cefixime, and cefaclor against various clinical isolates. me1206 was more active than the other cephems tested against staphylococci, streptococci, morganella morganii, pseudomonas cepacia, and flavobacterium meningosepticum and had the most potent activity against haemophilus influenzae and neiserria gonorrhoeae. the drug also showed a wide spectrum of activity against other gra ...19911929344
community acquired flavobacterium meningosepticum pneumonia and septicaemia. 19921734203
respiratory infection caused by flavobacterium meningosepticum. 19901968556
microbial endo-beta-n-acetylglucosaminidases acting on complex-type sugar chains of glycoproteins.the activity and substrate specificity of endo-beta-n-acetylglucosaminidase [glycopeptide-d-mannosyl-n4-(n-acetyl-d-glucosaminyl)2-asparagine 1,4-n-acetyl-beta-glucosamino-hydrolase, ec] obtained from mucor hiemalis (endo-m) was compared with that of the enzyme obtained from flavobacterium meningosepticum (endo-f), which is the only enzyme available that acts on the complex oligosaccharides of asparagine-linked sugar chains in glycoproteins. they showed almost the same activities toward ...19911718952
sinusitis and bacteremia caused by flavobacterium meningosepticum in a sixteen-year-old with shwachman diamond syndrome. 19921630865
characterization of a prolyl endopeptidase from flavobacterium meningosepticum. complete sequence and localization of the active-site serine.a prolyl endopeptidase was purified from flavobacterium meningosepticum. it was digested with trypsin. two oligonucleotides, based on tryptic peptide sequences and used in pcr experiments, amplified a 300-base pair (bp) fragment. a 2.4-kilobase ecori fragment that hybridized to the 300-bp probe was cloned in lambda zap and sequenced from both strands. it contains a reading frame of 2115 bp, encoding the complete protein sequence of 705 amino acids. ion-spray mass spectrometry experiments demonst ...19921569074
flavobacterium meningosepticum peptide:n-glycosidase: influence of ionic strength on enzymatic activity.flavobacterium meningosepticum peptide:n-glycosidase-mediated deglycosylation of n-linked glycan strands of glycoproteins has been found to be strongly influenced by the ionic strength of the assay medium. by use of a modification of a previously published assay procedure for quantitative analysis of glycan release we have been able to improve reproducibility and thus to compare the extent of deglycosylation achieved under a variety of conditions of ionic strength. we have observed that enzyme a ...19921560183
[a case of hypersensitivity pneumonitis caused by a humidifier].a 58-year-old woman was admitted complaining of dry cough and exertional dyspnea. physical findings, chest x-ray films, chest ct scan and respiratory function tests were suggestive of interstitial pneumonia. transbronchial lung biopsy showed specific findings of hypersensitivity pneumonitis. as a result of positive provocation test using her home humidifier, a diagnosis of humidifier lung was made. many microorganisms including flavobacterium meningosepticum were cultured from the water left in ...19921464990
use of resorufin-labelled n-glycopeptide in a high-performance liquid chromatography assay to monitor endoglycosidase activities during cultivation of flavobacterium meningosepticum.peptide-n4-(n-acetyl-beta-glucosaminyl) asparagine amidase f (pngase f) and endo-beta-n-acetyl glucosaminidase f (endo f) activities were monitored during cultivation of flavobacterium meningosepticum using a new fluorescence-hplc procedure based on a commercially available substrate. the pngase f activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while endo f continuously increased until late stationary phase. pngase f obtained at the ...19921422135
occurrence of alpha 1-2-fucosylation in membrane glycoproteins of morris hepatoma 7777 but not in liver. aberrant type of fucosylation in a malignant tissue.a comparative study was undertaken to characterize the linkages of l-fucose in n-glycans of plasma membrane glycoproteins from morris hepatoma 7777, host liver and kidney cortex, as well as from rat serum. after in-vivo radiolabelling of rats with l-[6-3h]fucose, the asparagine-linked carbohydrate chains were released from delipidated plasma membrane glycoproteins, as well as from serum glycoproteins, by enzymic digestion with peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase from flavob ...19921396674
hospital infections with pigmented water bacteria.two types of cultures normally expected to be sterile, i.e., urine and blood, were chosen for review at a large university hospital. the bacteria isolated from urine cultures during 1966-70 and from blood cultures from 1966-73 are listed. two organisms, flavobacterium meningosepticum and pseudomonas cepacia, were selected for more detailed study and review. the antibiotic susceptibility patterns for these two organisms are presented.19751236618
flavobacterium meningosepticum infection in a neonatal outbreak of infections due to flavobacterium meningosepticum type c in a neonatal intensive care unit is described. during a period of two weeks, two infants developed meningitis and a third was colonized in the respiratory tract and had transient bacteremia. the two meningitis patients were treated with clindamycin, rifampicin and cefotaxime systemically, plus rifampicin intraventricularly. bacteriological eradication was achieved within 48 h, and both infants recovered from the meningitis w ...19892504590
pneumonia and meningitis caused by a new nonfermentative unknown gram-negative isolates of an unclassified bacterium resembling flavobacterium spp. were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, protein profile analysis, and serologic data. the unclassified isolates were differentiated from flavobacterium meningosepticum, flavobacterium odoratum, flavobacterium balustinum, flavobacterium strain iib, chromobacterium violaceum, aquaspirillum serpens, and pseudomonas spp. the bacterium ...19892504766
outbreak of nosocomial flavobacterium meningosepticum respiratory infections associated with use of aerosolized polymyxin b.flavobacterium meningosepticum is an uncommon cause of adult nosocomial infection. on a medical/surgical intensive care unit we recently encountered an adult outbreak of respiratory colonization and infection caused by this organism, which was associated with the prophylactic use of aerosolized polymyxin b that had been used in an attempt to abort an outbreak of infection caused by highly resistant strains of pseudomonas aeruginosa. twenty isolates (95% from respiratory secretions) of f. meningo ...19892545119
purification of the oligosaccharide-cleaving enzymes of flavobacterium meningosepticum.four oligosaccharide chain-cleaving enzymes, including two new endoglycosidases distinct from endo-beta-acetylglucosaminidase (endo) f1, have been identified and purified to homogeneity from cultural filtrates of flavobacterium meningosepticum. fplc-directed hydrophobic-interaction chromatography in conjunction with high-resolution ion-exchange chromatography provided a more simple, rapid method for the isolation of endoglycosidase f1, f2 and f3, and the amidase, peptide-n4-n-acetyl-beta-d-gluco ...19911794038
prolyl endopeptidase from flavobacterium meningosepticum: cloning and sequencing of the enzyme gene.the prolyl endopeptidase [ec] gene of flavobacterium meningosepticum was cloned in escherichia coli with the aid of an oligonucleotide probe which was prepared based on the amino acid sequence. the hybrid plasmid, pfpep1, with a 3.5 kbp insert at the hincii site of puc19 containing the enzyme gene, was subcloned into puc19 to construct plasmid pfpep3. the whole nucleotide sequence of an inserted hincii-bamhi fragment of plasmid pfpep3 was determined by the dideoxy chain-terminating met ...19911840588
methods for identification of flavobacteria.published reports disagree on the best features for detecting and distinguishing between flavobacterium meningosepticum (biovar iia) and flavobacterium species cdc group iib (biovar iib; flavobacterium indologenes). this report discloses that at least some of these disagreements may reflect the methods used. to detect production of indole, a modified kovács reagent (not ehrlich) and a buffered tryptophan medium were optimal, but not all strains of these two biovars produced indole. to distinguis ...19892685029
[a case of subacute bacterial endocarditis due to flavobacterium meningosepticum (author's transl)]. 19751239477
[clinical and bacteriological study on flavobacterium meningosepticum (author's transl)].the present study concerns in vitro observations on the susceptibility to antibiotics and chemotherapeutic agents of the strains of f. meningosepticum isolated from various clinical specimens at the clinical laboratory of juntendo university hospital and the assessment of their clinical significance of the patient, when they are isolated from clinical specimens. 1) fifty-two % of the strains was isolated from sputum, 20% from urine and 8% from pus and exudate. only 2 strains were obtained from b ...1977857060
peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f cannot release glycans with fucose attached alpha 1----3 to the asparagine-linked n-acetylglucosamine residue.the ability of peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f (pngase f) from flavobacterium meningosepticum and pngase a from sweet almonds to deglycosylate n-glycopeptides and n-glycoproteins from plants was compared. bromelain glycopeptide and horseradish peroxidase-c glycoprotein, which contain xylose linked beta 1----2 to beta-mannose and fucose linked alpha 1----3 to the innermost n-acetylglucosamine, were used as substrates. in contrast to pngase a, the enzyme from f. meningo ...19911868849
[flavobacterium meningosepticum infection in a 19-month-old girl]. 1979460114
[neonatal meningitis by "flavobacterium meningoseptium" (author's transl)].we informed a case of neonatal meningitis caused by "flavobacterium meningosepticum", the first to be described in our country, taking into consideration its ethioepidemiology and therapy.1978566064
protection of mice from lethal endotoxemia by use of an ornithine-containing lipid or a serine-containing lipid.the effects of an ornithine-containing lipid [alpha-n-(3-acyloxyacyl)-ornithine (orn-l)] or a serine-containing lipid [alpha-n-(3-acyloxyacyl)-serine (ser-l)] from flavobacterium meningosepticum on lethal endotoxemia in mice were examined. when 500 micrograms of orn-l was intravenously administered 1 h before intravenous administration of a lethal dose of endotoxin, none of the mice died. the protective effect of ser-l was weaker than that of orn-l. light and electron microscopic studies demonst ...19911906840
[purulent meningitis due to flavobacterium meningosepticum in cameroonian children].following a number of reports of purulent csf specimens positive for flavobacterium meningosepticum in pediatric patients in yaoundé, a prospective study was carried out in the department of pediatrics of the central yaoundé hospital from december 1988 through december 1989. the goals of this study were to determine the incidence of flavobacterium meningosepticum among infants and children with purulent meningitis, to discover the origin of this pathogen, and to examine its susceptibility to ant ...19911952709
flavobacterium meningosepticum keratitis successfully treated with topical trimethoprim-sulfamethoxazole. 19911985480
community-acquired flavobacterium meningosepticum meningitis, pneumonia and septicemia in a normal infant. 19912003060
synthesis and inhibitory activity of acyl-peptidyl-prolinalderivatives toward post-proline cleaving enzyme as nootropic agents.several prolinal derivatives were synthesized and examined for their inhibitory activity on post-proline cleaving enzymes from flavobacterium meningosepticum and bovine brain and their possible properties as nootropic agents. almost all the compounds tested inhibited the activity of both enzymes at low ic50 values of the order of nm, but a specificity difference was observed with alkylacyl-prolinal derivatives which strongly inhibited only the bacterial enzyme. prolyl-prolinal derivatives were t ...19902079633
optimized deglycosylation of glycoproteins by peptide-n4-(n-acetyl-beta-glucosaminyl)-asparagine amidase from flavobacterium meningosepticum.peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f(pngase f) from flavobacterium meningosepticum is a highly useful enzyme for the structural analysis of n (asparagine)-linked carbohydrate chains derived from glycoproteins. the enzyme was enriched using a published procedure [tarentino al, gomez cm, plummer th, jr (1984) biochemistry 1985:4665-71; tarentino al, plummer th, jr (1987) methods enzymol 138:770-78] and further purified by hydrophobic interaction hplc on a weak hydrophobic ts ...19902136346
peptide-n4-(n-acetyl-beta-glucosaminyl) asparagine amidase and endo-beta-n-acetylglucosaminidase from flavobacterium meningosepticum. 19873110550
molecular cloning and amino acid sequence of peptide-n4-(n-acetyl-beta-d-glucosaminyl)asparagine amidase from flavobacterium meningosepticum.a 3,000-base pair ecori fragment containing the flavobacterium meningosepticum gene for peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase was cloned into the bluescript plasmid vector and expressed in escherichia coli. the gene consists of an open reading frame of 1,062 base pairs coding for a 354-amino acid protein; the first 40 amino acids are presumed to be the natural secretory signal sequence, with the remaining 314 amino acids (34,779 da) representing the catalytically active prote ...19902182634
cloning and expression of peptide-n4-(n-acetyl-beta-d-glucosaminyl)asparagine amidase f in escherichia coli.the peptide-n4-(n-acetyl-beta-d-glucosaminyl) asparagine amidase f (pngase f) gene from flavobacterium meningosepticum was cloned into a high copy number escherichia coli plasmid. levels of pngase f activity produced in cultures of the recombinant strain were up to 100-fold higher than those obtained in cultures of f. meningosepticum. the complete pngase f gene sequence was determined. comparison of the predicted amino acid sequence of pre-pngase f to the n-terminal sequence of the native mature ...19902182635
[flavobacterium meningosepticum meningitis. clinical and bacteriological study of 2 cases treated in senegal]. 1977884786
molecular cloning and heterologous expression of n-glycosidase f from flavobacterium meningosepticum.n-glycosidase f (peptide-n4-(n-acetyl-beta-glycosaminyl)asparagine amidase; ec catalyzes the cleavage of n-glycosidically linked carbohydrate chains between n-acetylglucosamine and asparagine. the structural gene was isolated by screening a flavobacterium meningosepticum genomic dna library in lambda gt10 with oligonucleotides, deduced from partial amino acid sequences of the protein. a clone with an open reading frame of 1062 bases was obtained. the amino acid sequence reveals a 42-re ...19902203781
[neonatal bacterial meningitis: etiological agents in 109 cases during a 10 year period].the etiology of purulent meningitis was investigated in 109 newborn infants admitted in a neonatal intensive care unit throughout a ten year period. bacterial pathogens were isolated from the csf in 57 (52.2%) neonates. there was a predominance of gram-negative bacilli isolated in 38 (34.9%) neonates. gram-positive cocci were isolated from csf in only 12 (11.0%) neonates. microorganisms associated with nosocomial septicemia and meningitis in neonates--klebsiella sp, salmonella sp. enterobacter s ...19921308405
occurrence of 2- and 3-hydroxy fatty acids in high concentrations in the extractable and bound lipids of flavobacterium meningosepticum and flavobacterium iib.the major hydroxy fatty acids of cellular lipids in flavobacterium meningosepticum and flavobacterium sp. king's group uub were identified as 2-hydroxy 13-methyltetradecanoic, 3-hydroxy 13-methyltetradecanoic, 3-hydroxy palmitic, and 3-hydroxy 15-methylhexadecanoic acids using gas chromatography-mass spectrometry and gc-mass fragmentography. the concentration of these hydroxy fatty acids comprised up to 30-40% of the total extractable and 20-30% of the bound lipid fatty acids, respectively. from ...1976994759
flavobacterium meningosepticum meningitis: report of selected aspects.two patients developed meningitis due to flavobacterium meningosepticum. because of resistance to most available antimicrobial agents, intravenous and intraventricular erythromycin was administered to one patient. subsequent development of resistance to erythromycin prompted the use of intravenous and intraventricular rifamycin, which eventually resulted in cure. diagnosis of f. meningosepticum meningitis in the second patient was delayed, and appropriate therapy was not administered until 4 day ...1978708021
peptide: n-glycosidase f: studies on the glycoprotein aminoglycan amidase from flavobacterium meningosepticum.peptide: n-glycosidase from flavobacterium meningosepticum was isolated in a homogeneous state and its physico-chemical characterization was accomplished. the reliability of the previously recorded assay procedures was assessed. using an octaglycopeptide derived from ovomucoid a rapid and sensitive fplc method was developed for the assay of enzymatic activity. peptide: n-glycosidase was found to effect deglycosylation of glycoproteins bearing complex and/or multiantennary glycans even in their n ...19892630586
[clinical evaluation of timentin in intensive care].16 patients admitted to a micu were treated with timentin (ticarcillin + clavulanic acid) for bacterial infections: 11 cases of pulmonary infection, 3 cases of urinary tract infection, 2 septic shocks, 3 septicemias and 1 case of multifocal infection. the pathogens considered as a firmly established cause of infection were: 5 acinetobacter, 12 pseudomonas, 3 serratia, 4 klebsiella, 1 e. coli, 2 proteus, 1 providencia, 1 staphylococcus aureus, 1 staphylococcus epidermidis, 1 streptococcus d and 1 ...19863534719
b-lymphocyte mitogenicity and adjuvanticity of an ornithine-containing lipid or a serine-containing ornithine-containing lipid (orn-l) or a serine-containing lipid (ser-l) from flavobacterium meningosepticum exhibited strong mitogenicity for the splenocytes from both lps-responder c3h/heslc and lps-low-responder c3h/hej mice. the potency of the lipoamino acids was the same as that of lps for responder mice. the lipoamino acids were b-lymphocyte mitogens. furthermore, orn-l or ser-l exhibited strong adjuvanticity. compared with the adjuvanticity of lps, the activity of orn-l was rather high. ...19911778427
fatal pneumonia caused by flavobacterium meningosepticum. 19892654863
flavobacterium meningosepticum meningitis in a neonate. 19892699316
successful eradication of flavobacterium meningosepticum neonatal meningitis with ceftizoxime. 19911895501
identification of distinct endoglycosidase (endo) activities in flavobacterium meningosepticum: endo f1, endo f2, and endo f3. endo f1 and endo h hydrolyze only high mannose and hybrid glycans.flavobacterium meningosepticum endo-beta-acetyl-glucosaminidase f preparations have been resolved by hydrophobic interaction chromatography on tsk-butyl resin into at least three activities designated endo f1, endo f2 and endo f3 each with a unique substrate specificity. the 32-kda endo f1 protein is the principle component representing in excess of 95% of most earlier and currently available commercial endoglycosidase preparations, the remainder being a mixture of five proteins from 32 to 43 kd ...19911899092
antimicrobial susceptibility of flavobacterium meningosepticum strains identified by dna-dna hybridization.antimicrobial susceptibilities of 52 strains of flavobacterium meningosepticum were determined by an agar plate dilution technique and by a tablet diffusion method. no useful susceptibility differences between the two main dna relatedness groups within f. meningosepticum were found. all strains produced beta-lactamase and were resistant to most beta-lactam antibiotics. ciprofloxacin, rifampicin and clindamycin appear to be the most promising for treating infections caused by f. meningosepticum.19873591313
[production of hydroxy and oxo fatty acids by microorganisms as a model of adipocere formation].microbial synthesis of hydroxy and oxo fatty acids was studied as one of the model of experimental adipocere formation. conversion of various fatty acids into 10-hydroxy and 10-oxo fatty acids by micrococcus luteus was also studied. fatty acids possessing cis-9-unsaturated forms were converted into 10-hydroxy and 10-oxo fatty acids. on the other hand, enoic acids possessing trans-9-unsaturated form or the ones which do not have double bond at the 9 -carbon position were inactive as substrates. 1 ...19911905675
cutaneous infection caused by flavobacterium meningosepticum. 19892909636
flavobacterium meningosepticum infections in a neonatal intensive care outbreak of nosocomial infections by flavobacterium meningosepticum in a neonatal intensive care unit is described. during a period of eleven weeks two patients presented with septicaemia and meningitis. in addition, nine patients were found to be colonized and three of these neonates developed septicaemia. the infected patients were treated with clindamycin and piperacillin. all the patients survived, but the neonates with meningitis developed hydrocephalus. an extensive bacteriological scre ...19892919524
[a case of neonatal meningitis due to flavobacterium meningosepticum successfully treated with cefmetazole and cefotaxime].a 3-day-old male infant, weighing 3,413 g and a gestational age of 38 weeks developed neonatal meningitis due to flavobacterium meningosepticum. treatment with cefmetazole and cefotaxime led him to a complete recovery without neurologic deficit. of 82 previously published cases under 1 year old, 41 cases died and 16 of survivors developed hydrocephalus because the organism was resistant to many antibiotics. therapy of meningitis due to the organism has not been standardized but the early laborat ...19883063849
sepsis from flavobacterium meningosepticum, an uncommon pathogen with unusual susceptibility patterns.flavobacterium meningosepticum, because of its unusual susceptibility patterns and predilection for debilitated or immunocompromised hosts, can be a dangerous pathogen. identification of the organism and proper susceptibility studies, which are essential in designing antibiotic therapy, helped in the selection of a regimen that proved curative in this case.19863704713
deglycosylation of gonadotropins with an endoglycosidase.a commercially available endoglycosidase (n-glycanase, genzyme, boston, mass.) purified from flavobacterium meningosepticum with a specificity for cleaving asparagine-linked carbohydrate moieties in glycoproteins was tested on several pituitary and chorionic gonadotropins as substrates. all intact hormones tested were resistant to the action of the enzyme as were all beta subunits from the respective gonadotropins. all alpha subunits, however, were susceptible to the enzyme as evidenced by a dec ...19863080756
phenotypic and genetic relationships of so-called moraxella (pasteurella) anatipestifer to the flavobacterium/cytophaga moraxella (or pasteurella) anatipestifer and members of the flavobacterium/cytophaga group exhibit remarkable common features: lack of flagellation, low guanine + cytosine content of the chromosomal dna, production of menaquinones and branched-chain fatty acids, absence of carbohydrate fermentation, and similar patterns of hydrolytic enzymes. using the renaturation method of dna:dna hybridization two urease-negative european isolates and the urease-positive type strain (which was isola ...19863739211
diabetic bb/wor rat haptoglobin exhibits a probable structural abnormality in asn-linked this study we demonstrate that haptoglobin, a serum glycoprotein secreted by the liver, has altered structure in the bb/wor diabetic rat. sds-page of haptoglobin (a tetramer composed of two glycosylated beta-chains each containing two sites for asn-linked oligosaccharides connected by disulfide bonds with two nonglycosylated alpha-chains) clearly shows that the beta-chain of haptoglobin from diabetic rats is smaller than normal, with a molecular mass of 39 instead of 40 kda. both acute and ch ...19912029525
oral ciprofloxacin therapy of infection caused by multiply resistant bacteria other than pseudomonas aeruginosa.of 125 patients treated with ciprofloxacin at the columbia-presbyterian medical center, new york, 34 had infections due to bacteria other than pseudomonas aeruginosa. the mean age of the patients was 50 years (19-88 years) and most had significant underlying disease. there were nine lower respiratory infections, eight urinary tract infections, eight soft tissue infections, three osteomyelitis, and three intra-abdominal infections. the pathogens were: escherichia coli, 7 (mean mic 0.07 mg/l); ser ...19863804906
transfer of glycerol by endo-beta-n-acetylglucosaminidase f to oligosaccharides during chitobiose core cleavage.n-linked oligosaccharides, when hydrolyzed by glycerol-containing preparations of endo-beta-n-acetylglucosaminidase (endo) f from flavobacterium meningosepticum were found to have glycerol attached to their reducing ends. the absence of a reducing end was confirmed by high-field 1h nmr spectroscopy, and the incorporated glycerol was verified through mass spectrometry and collisionally activated decomposition fast atom bombardment/mass spectrometry/mass spectrometry techniques. periodate oxidatio ...19863091595
various kinds of lipoamino acids including a novel serine-containing lipid in an opportunistic pathogen flavobacterium. their structures and biological activities on erythrocytes.lipoamino acids were found to represent 60% of the total extractable cellular lipids in flavobacterium meningosepticum and f. indologenes which are known to be opportunistic pathogens. the structures of the lipoamino acids were resolved by chemical and physicochemical methods. three kinds of lipoamino acids were included in the lipid of flavobacterium: 3-hydroxyisoheptadecanoic acid amide-linked to serine and esterified to isopentadecanoic acid, and 3-hydroxyisoheptadecanoic acid amide-linked to ...19883123236
[flavobacterium meningosepticum meningitis in a newborn infant--a case report]. 19883167662
flavobacterium meningosepticum infection, in a 32-day-old child on acute peritoneal dialysis, treated with ciprofloxacin. 19892496073
flavobacterium meningosepticum bacteremia in an adult with acute leukemia. use of rifampin to clear persistent infection.a case of flavobacterium meningosepticum bacteremia complicating the course of a patient with leukemia is described. the patient was treated successfully when rifampin was added to the antibiotic therapy. unusual organisms should be considered as agents of infection in immunocompromised hosts and susceptibility testing with drugs not commonly employed for gram-negative rods should be performed in complicated cases.19863455909
[neonatal meningitis caused by flavobacterium meningosepticum: a case report]. 19744215850
the mechanism of experimental adipocere formation: hydration and dehydrogenation in microbial synthesis of hydroxy and oxo fatty acids.the enzyme preparations from flavobacterium meningosepticum solubilized by sonication catalyzed not only hydration of oleic acid to produce 10-hydroxystearic acid but dehydrogenation of this product. the mechanism of the hydration and dehydrogenation was proved by gas chromatography-mass spectrometry of 10-hydroxy and 10-oxostearic acids produced in the presence of d2o or h2(18)o. the activity of these enzymes was increased by preincubating flavobacterium meningosepticum with oleic acid.19883410394
flavobacterium meningosepticum meningitis in an adult. 19883189379
flavobacterium meningosepticum meningitis in a newborn infant. treatment with intraventricular erythromycin. 19744479533
antibiotic resistance pattern of flavobacterium meningosepticum. 19863489618
endoglycosidases from flavobacterium meningosepticum application to biological problems. 19892516226
a comparison of strains of king's group iib of flavobacterium with flavobacterium meningosepticum. 19744599090
[cerebrospinal meningitis due to flavobacterium meningosepticum in an infant]. 19734731040
use of trimethoprim-sulfamethoxazole in pediatric infections: relative merits of intravenous administration.trimethoprim-sulfamethoxazole (tmp-smz) has traditionally been employed as an oral formulation for infections in ambulatory pediatric patients. however, therapeutic concentrations of tmp and smz in serum and csf are more consistently attained after intravenous administration. serum half-life increases with the age of the child, and few significant toxic effects are observed with intravenous administration. either the necessity to optimize bioavailability because of the underlying seriousness of ...19873554455
flavobacterium meningosepticum from cases of meningitis in botswana and england.two strains of flavobacterium meningosepticum isolated from cases of meningitis are described. one was isolated in botswana from a man with an aplastic anaemia, the other in the uk from an infant who was probably infected in bangladesh. the severity of the infection, the characterization of the organism, and the possibility that infection may be found in the uk are discussed.19734800468
meningitis in a new born due to flavobacterium meningosepticum. 19715161544
genetic heterogeneity of flavobacterium meningosepticum demonstrated by dna-dna hybridization.dna-dna reassociation studies on 52 strains of flavobacterium meningosepticum showed two main hybridization groups about 40-55% interrelated and comprising 4 and 48 strains respectively. the larger group could be further divided into four subgroups if differences in thermal stability of the reassociated duplexes were taken into consideration. of a total of 20 strains isolated from cerebrospinal fluid, 18 were found in one subgroup of 28 strains, which also contained seven blood isolates. the res ...19873565016
phenotypic characterization of flavobacterium meningosepticum strains identified by dna-dna hybridization.fifty-two strains found to belong to flavobacterium meningosepticum on the basis of dna-dna hybridization analyses were characterized and found to be phenotypically homogeneous. all strains were oxidase, catalase, indole, gelatinase and beta-galactosidase positive, and produced acid from glucose, mannose, fructose, maltose and mannitol; nitrate was not reduced. f. meningosepticum could be differentiated from flavobacterium group iib by its ability to produce beta-galactosidase, and by the latter ...19873565017
flavobacterium meningosepticum from neonatal meningitis. 19705311075
meningitis caused by flavobacterium meningosepticum. the first epidemic outbreak of meningitis in the newborn in south america. 19705415050
identification and distribution of flavobacterium meningosepticum in clinical material.during the 19 year period ending december 1984, 82 (1.7%) of 4840 strains of gram-negative non-fermentative bacteria submitted to the national collection of type cultures (nctc) for computer-assisted identification were flavobacterium meningosepticum. these figures suggest either that f. meningosepticum occurs only rarely in clinical material in the uk, or that when it does occur it is not always recognized. the sources from which the nctc strains were isolated are reported and also the characte ...19873571032
the sensitivity of flavobacterium meningosepticum to antibiotics at different temperatures. an in vitro study. 19675584517
flavobacterium meningosepticum isolated from outside hospital surroundings and during routine examination of patient specimens. 19695822968
[pharmacokinetic, bacteriological and clinical studies on imipenem/cilastatin sodium in neonates].pharmacokinetic, bacteriological and clinical studies on imipenem/cilastatin sodium (ipm/cs) were performed in neonates. the results were as follow: 1. a total of 27 patients consisting of 17 mature and 10 immature infants were treated with ipm/cs. each dose was 20 mg/20 mg/kg, and it was administered 2 approximately 3 times daily, in a 1-hour intravenous drip infusion for 3 approximately 12 days. the clinical efficacy of ipm/cs in 10 patients with bacterial infections (2 with sepsis, 3 with sus ...19892746857
crossed immunoelectrophoretic analysis of flavobacterium meningosepticum dna hybridization groups i and means of crossed immunoelectrophoresis and crossed-line immunoelectrophoresis, antigens were compared from four strains and five strains, respectively, of flavobacterium meningosepticum dna hybridization groups i and ii. two reference antisera raised against the pooled antigens of each of the two dna groups were used. the two reference antigen-antibody systems produced 81 and 51 immunoprecipitates, respectively, and within each group nearly all antigens were common to all strains, giving matc ...19892751894
the importance of temperature for the growth of flavobacterium meningosepticum. 19666006195
[composition of the complex lipids of flavobacterium meningosepticum].the free lipids of flavobacterium meningosepticum were separated by thin layer chromatography, and the main lipid fractions were analysed by fab (fast atom bombardment) mass spectrometry. the major products were di-iso-c15- and iso-c15-iso-c17-phosphatidylethanolamine, and two ninhydrin + and phosphorus- fractions. the structures of the latter two fractions were established as ornithine lipids by using mike (mass ions kinetic energy) mass spectrometry, gc/ms (gas chromatography coupled with mass ...19883408592
flavobacterium meningosepticum: a probable cause of meningitis in a cat. 19744450429
glycosylation of the mammalian alpha 1-adrenergic receptor by complex type n-linked oligosaccharides.the binding subunit of the alpha 1-adrenergic receptor has been identified as an mr = 80,000 peptide in several tissues. adsorption of the alpha 1-adrenergic receptor to a wheat germ agglutinin lectin-agarose resin suggests that the receptor protein is glycosylated. in this study, we investigated the nature of the carbohydrate chains linked to the alpha 1-adrenergic receptor peptide. the alpha 1-adrenergic receptor from ddt2 mf-2 smooth muscle cell and rat brain membranes was photolabeled with 1 ...19872824978
demonstration of peptide:n-glycosidase f activity in endo-beta-n-acetylglucosaminidase f preparations.endo-beta-n-acetylglucosaminidase f preparations from flavobacterium meningosepticum have been found to contain peptide:n-glycosidase activity. only the second activity, designated as peptide:n-glycosidase f, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding carbohydrate-free peptide containing aspartic acid and of an intact oligosaccharide with a di-n-acetylchitobiosyl moiety at the reducing end. ...19846206060
flavobacterium meningosepticum ventriculitis: in vivo and in vitro results with the combinations rifampicin-erythromycin and mezlocillin-cefoxitin.a case of flavobacterium meningosepticum ventriculitis is described in which the failure of therapy with a combination of rifampicin and erythromycin is attributed to inadequate antibiotic levels in cerebrospinal fluid. the successful eradication of the organism was achieved with the use of mezlocillin and cefoxitin given by the intravenous and intraventricular route. in vitro sensitivity tests of recently isolated strains of flavobacterium meningosepticum suggested that the combination mezlocil ...19826217065
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