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isolation and purification of flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of streptococcus mutans.studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of streptococcus mutans, streptococcus sanguis, and streptococcus sp. and their susceptibilities to dextranases. among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by s. mutans omz 176, which could be important in formation of dental plaques, was the most resistant. by enrichment cul ...1975370
high ph ammonia toxicity, and the search for life on the jovian planets.jovian plants have enviroments apparently suitable for the evolution of life, but nevertheless, present severe challenges to organisms. one such challenge arises from the presence of ammonia. ammonia is an efficient biocide, its effect being dependent on ph as well as on concentration. the effects of ph and ammonia concentration were studied separately, where possible, on a variety of organisms, including some isolated from natural enviornments of high ph and/or ammonia concentration. escherichi ...19751698
a comparative study between a chondroitinase b and a chondroitinase ac from flavobacterium heparinum: isolation of a chondroitinase ac-susceptible dodecasaccharide from chondroitin sulphate b.a chondroitinase that degrades only chondroitin sulphate b was isolated from flavobacterium heparinum, and separated from a constitutive chondroitinase ac also present in extracts of f. heparinum. the enzyme acts only on chondroitin sulphate b, producing oligo- and tetra-saccharides, plus an unsaturated 4-sulphated disaccharide (deltadi-4s). the oligosaccharide fraction (mol. wt. 3000) is susceptible to chondroitinase ac, producing mainly deltadi-4s. the chondroitinase b is distinguished from ch ...19752158
chondroitinase c from flavobacterium heparinum.a chondroitinase that acts upon chondroitin sulfate c and hyaluronic acid was isolated from flavobacterium heparinum. this enzyme was seperated from constitutional chondroitinase ac and an induced chondroitinase b also present in extracts of f. heparinum previously grown in the presence of chondroitin sulfates a, b or c. the enzyme acts upon chondroitin sulfate c producing tetrasaccharide plus an unsaturated 6-sulfated disaccharide (delta di-6s), and upon hyaluronic acid producing unsaturated no ...19762603
serum bactericidal activity in the horseshoe crab, limulus polyphemus.serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ...197611188
properties and biodegradation of a bioemulsifier from corynebacterium hydrocarboclastus.an extracellular polymer was produced by continuous fermentation of corynebacterium hydrocarboclastus on kerosene in a 24 liter reactor. this polymer was composed of protein, lipid, and carbohydrates. the polymer possessed surface active properties, and had two critical micelle concentrations. its effectiveness was quite comparable to the effectiveness of synthetic surface active agents such as tween 80 and span 20; however, its efficiency was much lower. the polymer also had emulsifying propert ...197719111
carboxypeptidase displaying differential velocity in hydrolysis of methotrexate, 5-methyltetrahydrofolic acid, and leucovorin.an enzyme that catalyzes the hydrolysis of folic acid and the antifolate methotrexate nearly 20 times more rapidly than the hydrolysis of 5-methyltetrahydrofolate was extraced from a gram-negative bacterium tentatively identified as a flavobacterium sp. the enzyme was purified 500-fold and found to have a molecular weight of about 53,000. apparently a metallo-enzyme, it is inhibited by citrate and ethylenediaminetetraacetic acid (edta). ca2+, co2+, mg2+, and zn2+ reverse inhibition by edta, wher ...197826657
extracellular accumulation of a new amino acid, o-2-hydroxypropylhomoserine, from 1,2-propanediol by flavobacterium rigense.during an investigation of microorganisms utilizing petrochemicals, a strain identified as flavobacterium rigense was found to accumulate a new amino acid in a medium containing 1,2-propanediol as the sole carbon source. cultural conditions for the accumulation of the product were investigated, and as a result, the yield was increased to 2.8 mg/ml after a 5-day incubation in a medium containing 8% 1,2-propanediol. the pure amino acid was isolated, and its structure was investigated. elemental an ...197828076
enzymic lysis and structure of the cell walls of the oral streptococcus mutans bht. 197944184
studies on the biodegradation of nonionic surfactants applied in the polyester fiber industry. i. activated sludge bacteria degrading the surfactants.the paper presents characteristics of 76 strains of bacteria capable of utilizing nonionic surfactants cirrasol fp, cirrasol sf 200 and cirrasol tcs as the source of carbon. the strains were isolated from two activated sludges adapted to the purification of wastes containing the above compounds at concentration 150--200 mg/l. the isolated strains belonged to the genera: achromobacter, alcaligenes, arthrobacter, flavobacterium, mycobacterium, nocardia, pseudomonas and xanthomonas. with load 0.11 ...197662497
amikacin, an aminoglycoside with marked activity against antibiotic-resistant clinical isolates.a total of 319 clinical isolates known to be resistant to one or more aminoglycoside antibiotics were tested for their susceptibility to 10 aminoglycosides. the percentages of isolates found by an agar dilution method to be susceptible were: amikacin, 83.7%; tobramycin, 41.4%; butirosin a, 33.2%; dideoxykanamycin b, 32.6%; gentamicin c, 27.3%; lividomycin a, 17.6%; neomycin b, 10.7%; paromomycin, 10.3%; kanamycin a, 10.0%; and ribostamycin, 7.2%. the effectiveness of the antibiotics was related ...197662814
[microbiological preparation of specifically tritiated androgens for the assay of steroid hydroxylases (proceedings)]. 197771100
the effect of cyclohexane derivatives on selection of bacterial groups forming activated sludge microflora.the effect of cyclohexanol, cyclohekxanon and cyclohexylamine on the selection of bacteria in a model population composed of bacteria isolated from activated sludge was examined. the initial population consisted of both gram-positive and gram-negative bacteria. the latter, which accounted for 90-97% of the population, belonged mainly to three pseudomonas groups and the enterobacteriaceae, vibrio-aeromonas, achromobacter-alcaligenes and flavobacterium groups. seven day growth in medium containing ...197880928
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. 197994750
bacterial utilization of cyclo(glycl-l-prolyl).3 strains of soil bacteria (2 achromobacter, 1 flavobacterium) were isolated by growth on cyclo(gly-l-pro) as carbon/nitrogen source. good growth required yeast extract supplements. utilization of cyclo(gly-l-pro) was inducible. many efforts failed to obtain active cell-free preparations. injected radioactive cyclo(gly-l-pro) was excreted intact by the albino rat; in contrast, injected radioactive h-gly-l-pro-oh was extensively metabolized.197895965
[products of bacterial destruction of sodium dodecyl sulphate].the products of na dodecyl sulphate destruction by the three bacterial cultures--pseudomonas aeruginosa, flavobacterium devorans, achromobacter guttatus--were examined. the cultures were shown to decompose na dodecyl sulphate in a similar way. the primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. products of bacterial destruction of alkyl sulphates did not show foam forming c ...1979117448
the clinical implications of contaminated pharmaceutical products. 1979117898
[drug sensitivity of glucose-nonfermenting bacteria other than pseudomonas aeruginosa (author's transl)]. 1979119072
enzymatic degradation of heparin-related mucopolysaccharides from the surface of endothelial cell cultures.when cultures of endothelial cells prelabeled with h2 -35-so4 are exposed to a purified preparation from induced flavobacterium heparinum containing heparinase and heparitinase activities, radioactivity accumulates in the supernatant medium. after further treatment in vitro with crude enzyme this material migrates, in part, as glucosamine (n,o-disulfated glucosamine), a break-down product characteristic of heparin and heparin-related mucopolysaccharides. after exposure of the cultures to the p ...1975123775
structural studies of heparitin sulfates.heparitin sulfate fractions with a large range in sulfate content were subjected to degradation by flavobacterium heparinase and by nitrous acid. the products obtained were fractionated by chromatography, characterized, and used to arrive at tentative structures for these complex polysaccharides. the heparitin sulfate chains examined appear to be composed of: 1. uninterrupted blocks of n-acetylglucosamine containing disaccharides; 2. larger blocks with a molecular weight range of 5000 to 6000 wh ...1975123778
[distribution of ubiquinones (coenzyme q) in gram negative bacillae].the coenzyme q system was examined on 55 strains of gram negative aerobic or facultatively anaerobic rods. no bacteria contain co-q7 nor co-q10. ubiquinone q8 predominates in flavobacterium and in enterobacteriaceae; q9 was the only homolog found in the pseudomonas, and predominates in the acinetobacter.1975126773
proteoglycans of soluble fraction of mouse mastocytoma.proteoglycans have been isolated from a high speed supernatant fraction of a mouse mastocytoma by procedures which should minimize alteration of the native protein-polysaccharide molecule. the methods used include in vivo labeling proteoglycans with 35s-sulfate, 3h-leucine and 3h-lysine, centrifugation of the tumor homogenate at 105,000 g, cetylpyridinium fractionation of the supernatant, and further purification of some of the fractions obtained by deae-cellulose column chromatography, gel filt ...1975127169
on the structure of heparitin sulfates. analyses of the products formed from heparitin sulfates by two heparitinases and a heparinase from flavobacterium heparinum.the analyses of the products formed from heparitin sulfates by the action of two heparitinases and a heparinase from flavorbacterium heparinum is reported. heparitin sulfates a and b are degraded by heparitinase i yielding two disaccharides, one of them composed of n-acetylucosamine and an unsaturated uronic, joined by alpha(1 lead to 4) linkage, and the other, with the same composition but with an o-sulfate at the hexosamine moiety. a third disaccharide is also formed from heparitin sulfate b, ...1976132967
the uses of degradative enzymes as tools for identification and structural analysis of glycosaminoglycans. 1977137129
action of chondroitinases. i. the mode of action of two chondroitinase-ac preparations of different origin.the modes of action of two chondroitinases-ac [ec 4.2.2.5] from arthrobacter aurescens and flavobacterium heparinum were examined. by comparison of the increase of viscosity and by analyses of digests using paper chromatography and gel filtration, it was shown that the arthrobacter enzyme (a-chase) degrades the substrate by a stepwise attack, while the flavobacterium enzyme (f-chase) exhibits a more random attack, though the first attack of both enzymes is of endo-type. further study was carried ...1976138676
[species composition of heterotrophic bacteria in the water of the rybinek reservoir].twenty four cultures of heterotrophic bacteria were isolated from the water of the rybinsk reservoir on a medium containing organic matter. bacteria belonging to the families pseudomonadaceae and achromobacteriaceae were most widely distributed; bacteria of the mycobacteriaceae family were encountered less often. predominating species which belonged to the genera pseudomonas, caulobacter, flavobacterium, mycobacterium, and corynebacterium were isolated from the central part of the reservoir. the ...1977142898
chemistry of heparitin sulfate and heparin from normal tissues and from patients with hunter syndrome.some structural features of heparitin sulfate excreted by patients with hunter syndrome are described. it is shown, with the aid of heparitinases and heparinase from flavobacterium heparinum, that the hunter heparitin sulfate is a very complex structure composed of nine different disaccharide units containing regions akin to normal heparitin sulfate and regions akin the heparin. two-thirds of the iduronic acid residues of hunter heparitin sulfate are devoid of sulfate, contrasting with heparin i ...1979153768
[new species of obligate-thermophilic bacteria of the flavobacterium thermophilum type]. 1978154607
structure of heparan sulphate oligosaccharides and their degradation by exo-enzymes.oligosaccharides obtained from heparan sulphate by nitrous acid degradation were shown to be degraded sequentially by beta-d-glucuronidase or alpha-l-iduronidase followed by alpha d-n-acetylglucosaminidase. structural analysis of the tetrasaccharide fraction showed the following. (1) n-acetylglucosamine is preceded by a non-sulphated uronic acid residue that can be either d-glucuronic of l-iduronic acid, but followed by a glucuronic acid residue. (2) the n-acetylglucosamine in the major fraction ...1979161508
identification of nonfermentative gram-negative bacteria in the clinical laboratory.a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ...1975163060
localization of selenium in bacterial cells using tem and energy dispersive x-ray analysis.bacteria isolated from lake sediment samples reduced sodium selenite to elemental selenium. finestructural observations were made on a number of different bacterial species cultured in the presence of sodium selenite. examination of escherichia coli and a pseudomonas species revealed electron-dense deposits of irregular shape, composed of smaller units, within the cytoplasm but not on the cell wall and cell membrane. cells of aeromonas and flavobacterium species exhibited conspicuous intranuclea ...1976175749
production of 13beta-alkyl-3-methoxy-8,14-seco-1,3,5(10),9(11)-gonatetraen-14beta-ol-17-ones and 13beta-alkyl-3-methoxy-8,14-seco-1,3,5(10),9(11)-gonatetraen-17alpha-ol-14-ones by microbial enzymes.for the purpose of producing hydroxy-keto-seco-steroids in which hydroxyl group is attached to a carbon atom having the r-configuration, numerous biochemically active microorganisms were tested without any success. the hydroxysteroid oxidoreductase enzymes of the investigated bacterial, yeast and fungal strains were suitable only for the production of 17beta-ol-14-one and 14alpha-ol-17-one derivatives. the required compounds were prepared by combinations of enzymatic reactions with chemical redu ...1975179287
purification and characterization of glycerate kinase from flavobacterium sp. n.c.i.b. 11171 [proceedings]. 1978208889
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
kinetic control of phosphoglycerate mutase from flavobacterium sp. grown on ethylene glycol.a species of flavobacterium able to oxidise ethylene glycol to pyruvate via glyoxylate, glycerate, 2-phosphoglycerate and phosphoenolpyruvate exploits phosphoglycerate mutase to initiate gluconeogenesis. partially purified phosphoglycerate mutase from this bacterium is independent of adenylate charge control but is activated by phosphoenolpyruvate. the possible significance of this regulation is discussed.1979227474
purification and properties of a beta-1,6-clucosidase from flavobacterium.an intracellular beta-1,6-glucosidase (beta-d-glucoside glucohydrolase, ec 3.2.1.21) was produced semiconstitutively by flavobacterium m64. this enzyme was purified 180-fold by fractionation with ammonium sulfate followed by chromatographies on carboxymethylcellulose, hydroxyapatite and sephadex g-100. the final preparation appeared homogeneous on disc electrophoresis on polyacrylamide gel. the molecular weight of the enzyme was determined to be ca. 59 000 by sephadex g-100 gel filtration and so ...1975235305
purification and properties of elastolytic enzyme from flavobacterium immotum.elastolytic enzyme was purified and crystallized from culture fluid of flavobacterium immotum no. 9-35. the purified enzyme was homogeneous on polyacrylamide gel electrophoresis. the molecular weight was determined by sephadex g-100 gel filtration to be 13,000. the isoelectric point was between ph 8.3 and 8.9. the optimum ph of the enzyme was 7.2 for elastolytic activity. the purified enzyme showed not only elastolytic activity, but also non-specific proteolytic activity against various other pr ...1975236995
differentiation of nonfermentative gram-negative bacilli in the clinical laboratory.a rapid and simplified system for the differentiation of nonfermentative gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. nonfermentative bacteria can be grouped initially by the motility, oxidase and of glucose reactions. this grouping simplifies the choice of additional tests for further identification. the additional tests included gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase act ...1977329428
microbial aggregate contamination of water lines in dental equipment and its control.water from some dental clinics has been examined and found to be discoloured, badly tasting and with a foul odour. moreover, brown or black flakes were often present in tap water, as well as in the water lines of dental equipment. examination by phase-contrast and electron microscope showed the flakes to consist of aggregated fungi and bacteria, and similar structures were found in a layer on the inner surfaces of the clinics water tubes and pipes. the ultrastructure of some aggregating microorg ...1977329637
retention of bacteria in liquid films at agar surfaces.the number of bacteria retained by agar dipslides immersed in bacterial suspensions was dependent solely on suspension population density and was unaffected by the nutrient status of the agar surface or liquid, disturbance of the liquid, or bacterial motility and chemotaxis.1977335974
evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, gram-negative rods.the ability of the expanded api-20e and the oxi-ferm system to identify 176 isolates of nonfermenting or oxidase-positive, gram-negative rods from 17 species or groups was studied. the expanded api-20e, without referral to a computer reference center, was able to identify 61.4% of the isolates to the species level. if reference to the computer center was utilized, an additional 25% could be identified. of the isolates examined, 13.6% were misidentified, partially identified, or assigned no ident ...1977338626
the identification of pseudomonads and related bacteria in a clinical laboratory.non-fermenting, catalase-positive gram-negative bacilli that grow on nutrient agar are often isolated in clinical laboratories. we have applied biochemical techniques appropriate to a typical clinical microbiology laboratory, and for the most part described in cowan and steel's manual for the identification of medical bacteria (cowan, 1974), to 428 clinical isolates and have evolved a scheme for their identification. organisms were subdivided into groups on the basis of three tests, namely the g ...1978351182
[infections due to chromobacterium, serratia, erwinia and flavobacterium (the so-called chromobacterioses)]. 1978361381
a review. taxonomic strategies for flavobacterium and related genera. 1978365848
retention of bacteria on chicken skin after immersion in bacterial suspensions. 1978365849
[investigation in vitro synergism on ampicillin with cloxacillin and dibekacin against glucose non-fermenting gram-negative bacilli and serratia (author's transl)]. 1978366194
primary structure of a chloramphenicol acetyltransferase specified by r plasmids.naturally occurring isolates of chloramphenicol-resistant bacteria commonly synthesise chloramphenicol acetyltransferase (ec 2.3.28; cat) in amounts which are sufficient to account for the resistance phenotype and often harbour plasmids which carry the structural gene for cat. the findings of cat in such diverse prokaryotes as proteus mirabilis, agrobacterium tumefaciens, streptomyces sp., and a soil flavobacterium has led to speculation concerning the origin and evolution of the more commonly o ...1979390404
35s-labeled dermatan sulfate. preparation of a substrate for enzymic degradation studies. 1979393392
degradation of organophosphorus and carbamate insecticides in the soil and by soil microorganisms. 1977402103
oxidase positive rods from cases of suspected gonorrhoea. a comparison of conventional, gas chromatographic and genetic methods of identification.genito-urethral specimens from 3260 women and 1170 men, with ailments suggestive of gonorrhoea, were examined for growth of oxidase positive rodshaped bacteria, as well as of gonococci. moraxella osloensis was identified in 26 cases (0.64 per cent of women and 0.43 per cent of men). three patients harboured phenylalanine negative (or weakly reacting) and tryptophan deaminase negative m. phenylpyrouvica and, in three cases, a flavobacterium species was detected. among six oropharyngeal specimens ...1977402788
bioconcentration of toxaphene by microorganisms. 1977405061
[infection caused by flavobacterium meningosepticum, with special reference to purulent meningitis in newborn infants]. 1977405436
numerical taxonomy of heavy metal-tolerant bacteria isolated from an estuary.a total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in chesapeake bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. in addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. twenty-three reference strains were also included in the numerical taxonomy study of these bacteria, bringing the grand total of strains examined to 324. all strains were examined for 112 bi ...1977411560
the history, biology, and taxonomy of the cytophaga group.the first section of this review covers the important characteristics of the genera cytophaga and sporocytophaga. the topics discussed include vegetative cell structure, the spreading habit, and degradation of macromolecules. a historical account of these two genera follows, together with a discussion on the definition of, and species differentiation with the genus cytophaga, and on the taxonomy of sporocytophaga. the third section deals with the relationships of the cytophagas with the flavobac ...1977413616
[isolation of bacteria designated as "group m-4f" (flavobacterium odoratum) from clinical materials (author's transl)]. 1979427603
purification of chondroitinase b and chondroitinase c using glycosaminoglycan-bound ah-sepharose 4b.chondroitinase b and chondroitinase c were separated from an extract of flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. chondroitinase c was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. the latter two activities were eliminated exclusively by passing the crude chondroitinase c fraction through a phosphono-cellulose column pre-equilibrated with 0.07m sodium phosphate buffer (ph 6.8). ch ...1979427837
the microbial degradation of a sulphanilamide-based herbicide (asulam) [proceedings]. 1979428664
flavobacterium odoratum: a species resistant to a wide range of antimicrobial agents.during the period 1966-77, 24 strains of flavobacterium odoratum were identified from among strains of gram-negative, non-fermentative bacteria submitted to the national collection of type cultures for computer-assisted identification. the f. odoratum strains showed resistance to therapeutic levels of gentamicin, tobramycin, amikacin, and carbenicillin as well as to several other antimicrobial agents generally useful in the treatment of infections caused by gram-negative, non-fermentative bacter ...1979429582
water-borne contamination of intrauterine pressure transducers.internal intrapartum fetal monitoring has been implicated as a source of maternal and fetal sepsis. we report water-borne contamination of a component of the intrauterine pressure transducer and suggest that this may be a potential source of nosocomial infection.1979434037
flavobacterium meningosepticum infections. 1979451641
[bacteria of current interest. 2. non-fermentative gram negative rods, with special reference to water insoluble yellow pigment producing bacteria]. 1979459114
[flavobacterium meningosepticum infection in a 19-month-old girl]. 1979460114
[role of adsorption in microbial colonization of volcanic deposits]. 1979470637
the sensitivity of gentamicin-resistant gram-negative bacilli to cefotaxime, other cephalosporins and aminoglycosides.the sensitivities of 80 gentamicin-resistant gram-negative bacilli to cefotaxime, cefuroxime, cefoxitin, cefamandole, cefazolin, tobramycin, netilmicin and amikacin were determined. amikacin was the most active amino-glycoside. however, the percentage sensitivity to cefotaxime of most of the species was higher than, or equal to any of the other antibiotics tested. cefotaxime was particulary active against providencia spp., serratia spp., klebsiella spp., and pseudomonas maltophilia, being 16 to ...1979478651
l-glutamine formation by flavobacterium rigense.a penicillin-resistant mutant of flavobacterium rigense designated as strain 703, ferm-p no. 3628, was obtained after ultraviolet treatment of f. rigense ferm-p no. 3556. the parent strain produces 0-2-hydroxypropylhomoserine from 1,2-propanediol. the mutant was found to be a good producer of l-glutamine. the physiological characteristics of strain 703 were different from the general group of l-glutamic acid-producing bacteria. strain 703 required l-tryptophan and thiamine but not biotin for its ...1979485142
chemical composition of streptococcus mutans cell walls and their susceptibility to flavobacterium l-11 enzyme.the susceptibility to a cell wall lytic l-11 enzyme from flavobacterium sp. and the quantitative and/or qualitative composition of the cell walls of some strains of cariogenic streptococcus mutans and a non-cariogenic strain of streptococcus mitis were determined. the purified cell walls of s. mutans strains hs-1 (serotype a), bht (b), nctc10449 (c), c67-1 (c), c67-25 (c), omz 176 (d), mt703 (e), mt557 (f), omz65 (g), and aht (g), and s. mitis cht contained glutamic acid, alanine, and lysine as ...1979502897
[six new serotypes of "flavobacterium meningosepticum" (author's transl)].six new serotypes (g to l) of flavobacterium meningosepticum are described herein. cross-reactions between the 12 serotypes, a to l (including the 6 serotypes a to f of owen and lapage, 1974) are described. sixty-six strains isolated in france (61 from strasbourg) belong to the following serotypes (with number of strains): a (1), f (4), g (51), h (1), i (1), j (6), k (1) and l (1). use of meningosepticum serotyping is recommended as an epidemiological tool.1979507635
bacterial metabolism of propane-1,2-diol.the pathway of propane-1,2-diol metabolism by a species of flavobacterium able to grow on the diol as the sole source of carbon was influenced by the degree of aeration of the growth medium. under strongly aerobic conditions the diol was exclusively catabolised to lactaldehyde by an initial diol oxidase, subsequently metabolised to pyruvate and then oxidised to co2 by the tricarboxylic acid cycle. under microaerophilic conditions some propane-1,2-diol was catabolised by the oxidase-initiated pat ...1979508808
airway colonization by flavobacterium in an intensive care unit.a total of 195 patients admitted to a respiratory-surgical intensive care unit became colonized with species of flavobacterium during a 70-month prospective study. by biochemical, cultural, and morphological criteria and a comparison of antibiotic susceptibilities, all patient isolates of flavobacterium were apparently related. the origin of these organisms was sought. flavobacterium were recovered from different water-associated areas of the hospital and from the hands of respiratory-surgical i ...1979511985
cellular fatty acid composition of pseudomonas paucimobilis and groups iik-2, ve-1, and ve-2.the cellular fatty acid composition of pseudomonas paucimobilis (iik-1) and group iik-2, ve-1, and ve-2 was determined by gas-liquid chromatography. the unnamed groups were readily distinguished from p. paucimobilis by cellular fatty acids. the data strongly suggest that these bacteria may be additional species of pseudomonas and flavobacterium.1979511989
meningitis caused by pseudomonas paucimobilis.this appears to be the first report of meningitis due to pseudomonas paucimobilis and the first report of a clinically significant isolate of this species in the uk. characteristics by which the species may be recognised are given, and attention is drawn to the possible confusion of ps. paucimobilis with other yellow-pigmented pseudomonads and flavobacterium species.1979512057
partial purification and properties of a bacterial isoamylase.isoamylase has been prepared by affinity chromatography of a commercial enzyme-preparation from a strain of cytophaga (also known as a flavobacterium or polyangium). the enzyme was not very stable, but the stability could be improved by calcium ions. the enzyme had a very low but significant activity on pullulan and on alpha-dextrins having maltosyl side-chains. this observation, which is contrary to previous reports, has been related to the specificity of isoamylase and other bacterial debranch ...1979526954
[role of population density in regulation of the number of bacteria in soil]. 1979530140
colonization of an amputation site by flavobacterium odoratum after gentamicin therapy.flavobacterium odoratum was isolated from the stumps of the amputated toes of a debilitated patient. gentamicin therapy and the poor state of health of the patient provided suitable conditions for this gentamicin-resistant species to multiply. when the gentamicin therapy was stopped, and as the patient's heal improved, f. odoratum gradually disappeared to be replaced by staphylococcus aureus. this appears to be the first report of the isolation of f. odoratum in australia, and it indicates the p ...1979530210
chloramphenicol resistance of three different flavobacteria.the chloramphenicol resistance of some flavobacteria was investigated comparatively. this resistance can be explained either by acetylation of chloramphenicol to o-acetylchloramphenicol via constitutively formed acetyltransferases, followed by cometabolic degradation (strain cb 60), or by limited uptake and total degradation (strain cb 6) by inducible enzymes or by other mechanisms (f. devorans). the mechanisms of resistance, cm-acetylation, cm-degradation and limited uptake are discussed.1979541254
evaluation of a class iii biological safety cabinet for enclosure of an ultracentrifuge.an evaluation of a special safety cabinet housing a high-speed centrifuge was made. the cabinet enclosed both the top access port and the drive and pumping machinery of the centrifuge. a titanium rotor was loaded with tubes containing a bacterial culture, weakened, and driven until rotor rupture occurred. there were several bent and broken components in the centrifuge, and bacteria leaked from the vacuum chamber. although the forces were sufficient to displace the cabinet, none of the test bacte ...1979543704
[flavobacterium meningosepticum (author's transl)]. 1977559147
[neonatal meningitis by "flavobacterium meningoseptium" (author's transl)].we informed a case of neonatal meningitis caused by "flavobacterium meningosepticum", the first to be described in our country, taking into consideration its ethioepidemiology and therapy.1978566064
characterization of a dinoflagellate (amphidinium carterae) dna.the dna of the photosynthetic dinoflagellate amphidinium carterae shows, by certain physico-chemical criteria two major components, a light satellite and a permanent dna-rna hybrid whose presence explains the unusual behaviour of amphidinium dna according to classical methods of analysis. the frequent presence of a slow-growing accompanying bacterium does not seem to interfere with any of these findings.1978572707
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
[flavobacterium meningosepticum as the cause of meningitis in a premature infant]. 1977611436
microbial metabolism of aliphatic glycols. bacterial metabolism of ethylene glycol.a species of flavobacterium isolated from pond water by its ability to grow aerobically on ethylene glycol as the role source of carbon initially oxidised the diol to glyoxylate via glycollate. the glyoxylate was metabolised by the glycerate pathway to acetyl-coa. the acetyl-coa was further metabolised by the tricarboxylic acid cycle plus malate synthase acting anaplerotically.1978620072
structural factors influencing the biodegradation of imides.comparative studies on the biodegradability of amides and imides are presented. low-molecular-weight imides of varying chain lengths (4, 6, 7, 8, 18, and 20 carbons) were biodegrable. n-alkyl substitution of amides and imides resulted in non-biodegrable derivatives when the amide portion was greater than two carbons in length. n-alkyl-substituted derivatives of acetamide or diacetamide, however, were biodegrable. several soil isolates, including aspergillus niger and species of flavobacterium an ...1978623472
flavobacterium meningosepticum as an opportunist.flavobacterium meningosepticum was isolated from the cerebrospinal fluid of an adult immunodeficient female. in spite of prompt therapy the patient succumbed to the infection. the opportunistic role of the organism is discussed.1978641195
bacterial oxidation of polyethylene glycol.the metabolism of polyethylene glycol (peg) was investigated with a synergistic, mixed culture of flavobacterium and pseudomonas species, which are individually unable to utilize pegs. the peg dehydrogenase linked with 2,6-dichlorophenolindophenol was found in the particulate fraction of sonic extracts and catalyzed the formation of a 2,4-dinitrophenylhydrazine-positive compound, possibly an an aldehyde. the enzyme has a wide substrate specificity towards pegs: from diethylene glycol to peg 20,0 ...1978646355
interactions between the diatom thallasiosira pseudonanna and an associated pseudomonad in a mariculture system.the marine diatom thallasiosira pseudonanna (3h) and several bacteria associated with it were isolated from batch cultures at the university of delaware mariculture facility. the interaction between the algae and each of the bacteria was investigated. one of the isolates, t827/2b (pseudomonas sp.), was incapable of surviving in f/2 culture medium unless the algae were present. when the algae and t827/2b were grown together in the f/2 medium, the bacterial growth was stimulated and the algal grow ...1978646360
carbenicillin plus cefamandole in the treatment of infections in patients with cancer.the combination of carbenicillin plus cefamandole was administered to 88 patients with cancer during 116 evaluable episodes of fever. the overall response rate to carbenicillin plus cefamandole for the 116 episodes was 57%. there were 60 documented infections, of which 60% responded to this combination of antibiotics. the response rate was only 43% in patients with pneumonia. the etiologic agent was identified during 38 infections, of which 74% responded to carbenicillin plus cefamandole. respon ...1978649998
bacteria from the gut of australian termites.the major gut bacteria of the worker caste of nine species of australian termites, belonging to four families, were isolated and identified to generic level. all species were either facultative anaerobes or strict aerobes. a correlation appears to exist between the major gut bacterium and the family to which the termite belongs. the major bacterium from the two lowest termites, mastotermes darwiniensis (family mastotermitidae) and cryptotermes primus (family kalotermitidae), was streptococcus; f ...1978655700
isolation of a novel sphingoglycolipid containing glucuronic acid and 2-hydroxy fatty acid from flavobacterium devorans atcc 10829.a new acidic sphingoglycolipid has been isolated from a gram-negative, glucose-non-fermentative (obligatory aerobic) bacterium, flavobacterium devorans atcc 10829, by thin-layer chromatography on silica gel after mild alkaline hydrolysis of the cellular lipids. chemical degradation studies, thin-layer chromatographic behavior, ir and mass-spectrometric analysis of the original and reduced glycolipid with lia1h4 revealed that the lipid contained glucuronic acid, long-chain bases, and fatty acids ...1978659393
[microorganisms that break down alkyl sulfates].the following bacteria assimilating alkyl sulphates as a sole source of carbon and energy were isolated from various substrates: pseudomonas, achromobacter, flavobacterium, citrobacter, enterobacter. the bacteria decomposed sodium dodecyl sulphate at a high rate, and some of them, industrial preparations of alkyl sulphates. the ability to assimilate alkyl sulphates was tested in 257 collection cultures belonging to different taxonomic groups. alkyl sulphates were found to be decomposed by hetero ...1978661643
spectrophotometric determination of l-lysine with l-lysine: alpha-ketoglutarate epsilon-aminotransferase. 1978677455
a comparative analysis of extreme thermophilic bacteria belonging to the genus thermus.several extreme thermophilic gram negative bacteria found in a thermally polluted river in belgium have been compared with thermus strains isolated from widely distant geographical areas. this analysis has become possible after the design of a new culture medium (162). all strains examined (including the isolate successively denominated flavobacterium thermophilum and thermus thermophilus) were found to be morphologically identical with strain yt-1 of thermus aquaticus. the cells are immotile, r ...1978678024
vitamin b6 biosynthesis and nucleoside effect in the resting cell system of flavobacterium sp. 238-7.as one of the attempts to find clues on precursors or intermediates of the vitamin b6 biosynthetic pathway, the resting cell system of flavobacterium sp. 238-7, a bacterium producing a high amount of the vitamin, was employed. among various compounds tested as an additive to the reaction mixture, l-glutamate and nucleoside derivatives increased the formation of vitamin b6, respectively. the possible mechanism of the participation of these compounds in vitamin b6 biosynthesis is discussed. the id ...1978690725
[flavobacterium meningitis]. 1978691327
[pyoventriculitis caused by flavobacterium meningosepticum; treatment with rifamycine]. 1978693224
in vitro activity of rosamicin and erythromycin against a group of nonfermenting gram-negative bacilli.the in vitro inhibitory activity of rosamicin and erythromycin against 283 strains of nonfermenting, gram-negative bacilli was determined by using a broth dilution procedure. rosamicin demonstrated greater activity than erythromycin against most strains tested. a number of species demonstrated significantly lower minimum inhibitory concentrations to rosamicin and would fall within the therapeutic range of the drug based on current pharmacological data.1978697353
a soil flavobacterium sp. that degrades sulphanilamide and asulam. 1978701200
flavobacterium meningosepticum meningitis: report of selected aspects.two patients developed meningitis due to flavobacterium meningosepticum. because of resistance to most available antimicrobial agents, intravenous and intraventricular erythromycin was administered to one patient. subsequent development of resistance to erythromycin prompted the use of intravenous and intraventricular rifamycin, which eventually resulted in cure. diagnosis of f. meningosepticum meningitis in the second patient was delayed, and appropriate therapy was not administered until 4 day ...1978708021
antimicrobial susceptibility of flavobacteria.antimicrobial susceptibility patterns of 28 clinical isolates of flavobacterium sp. were determined by standard disk diffusion technique and by antimicrobial dilution in agar. rifampin, clindamycin, trimethoprim-sulfamethoxazole, cefoxitin, and vancomycin are among the antimicrobial agents which may be clinically useful to treat infections caused by flavobacteria. all 28 isolates were resistant to erythromycin with minimal inhibitory concentrations of 32 mug/ml or more. currently recommended int ...1978708026
humidifier fever and endotoxin exposure.three cases of humidifier fever were detected in an office environment. flavobacteria were found in the contaminated water in a humidifier. after an experimental exposure, the three persons with previous symptoms suffered from fever and slight respiratory symptoms. a leucocytosis and an increase in the number of segmented white blood cells were found the day following the exposure. general immunoglobulins as well as antibodies to flavobacterium and endotoxin were slightly elevated in the exposed ...1978709796
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