cholinesterase activity of sperm from rams infected with brucella ovis.studied were the indices of cholinesterase activity of semen taken from 23 normal rams and 23 rams infected with brucella ovis, the latter being positive by the complement-fixation test and showing a varying deterioration of the semen production. the results obtained were processed biometrically. established were dependable differences. the cholinesterase activity of semen of brucellosis-affected rams proved four times higher than that of normal rams' semen: 39.45 +/- 5.49 microleter to2 for 1 h ...197722157
[biochemical study of serum from sheep infected with brucella ovis].blood serum was used of 49 rams and 20 ewew infected with brucell ovis. the study on the total protein and the protein fractions of rams revealed that there existed gamma-globulinemia, the percent of the gamma-globulin rise showing a positive correlation with the morphologic changes in the testes. the alfa-globulins were found to rise immediately following the experimental infecting of the sheep for about a month, after which they came back to normal. in experimental infection the activity of th ...197883049
[aspects related to the antigenic structure and serological specificity of brucellae phylogenetically related to s- and r-forms and dissociated into r-variants].the antigenic structures and serological properties of brucellae s- and r-forms, phylogenetically differentiated, and dissociated r-variants are studied by means of gel-precipitation, immunoelectrophoresis and fixation of the complement. the brucella suis 1330s, brucella suis 1330r, brucella ovis 02 and brucella abortus 99 are involved in the experiments. both specific and general antigenic structures are established in all strains studied, but only homologous antibodies from the antigen-antibod ...197888791
actinobacillus seminis infection in sheep in the republic of south africa. iii. growth and cultural characteristics of a. seminis.bacteriological tests were done on a large number of different strains of actinobacillus seminis and also, repeatedly, on the same culture or on different cultures taken periodically from the same donor animal. these tests were also applied to strains of a. seminis representing different serological types, which in turn were compared with strains of brucella ovis. the tests as applied proved that a. seminis strains have defined, morphological, staining, cultural and biochemical properties, altho ...197995214
epiphysitis in rams following vaccination against brucella ovis infection. 197898740
epididymitis in rams: effect of vaccination and culling on the clinical incidence of the disease.ram epidiymitis caused by brucella ovis can be effective controlled by an annual program combining rigorous culling clinically affected animals and appropriate vaccination of the remaining animals. yearling rams purchased or saved as replacement breeders should be immunized at 4-5 months of age by two injections of br. ovis bacterin spaced 3-6 weeks apart. annually, approximately one month prior to the breeding season, all rams, replacements and older breeders as well, should be carefully examin ...1979110514
comparison of agar gel precipitation and complement fixation test for the detection of brucella ovis infection.blood samples from 710 sheep raised in 20 different farms were examined for brucella ovis antibodies. thirty samples were positive with complement fixation, 35 samples with precipitation, and 58 samples with both tests. it has been concluded that the gel precipitation technique is adequate for screening but for individual testing both methods should be used simultaneously.1979113989
serodiagnosis of ram epididymitis by counterimmunoelectrophoresis, using brucella ovis surface r antigen.the counterimmunoelectrophoresis (cie) technique was developed as a diagnostic procedure for ram epididymitis caused by brucella ovis. cie test results with sera from naturally and experimentally infected male sheep compared favorably with those obtained by gel diffusion and complement fixation employing the same b. ovis surface r antigen. the main advantage of cie over gel diffusion consists in a significant reduction of the time required to detect precipitin formation, whereas both methods obv ...1979118983
observations on brucella ovis cft results. 1978286955
bacterial isolates associated with epididymitis in rams from idaho and eastern oregon flocks.testicles from 47 rams with palpable lesions of epididymitis were subjected to bacterial culture. of the testicles examined by culture technique, 49% were positive for bacterial growth. the 2 most frequent isolates were actinobacillus actinomycetemcomitans (23.4% of the testicular pairs) and brucella ovis (14.9% of the testicular pairs). heretofore a actinomycetem-comitans has not been reported as a cause of epididymitis in rams.1979453669
brucella ovis in merino rams in western australia. 1979464944
actinobacillus seminis infection in sheep in the republic of south africa. i. identification of the problem.a clinical palpation and semen smear examination of 647 rams submitted to the regional veterinary laboratory during 1967 revealed that 42 (6,5%) of these animals had clinical epididymitis or orchitis, 6 (0,9%) showed other types of genital lesions and 98 (15,1%) suffered from subclinical genital infection. a. seminis and a. seminis-like organisms were isolated from semen specimens of 18 out of 35 rams with clinical epididymitis or orchitis, 25 out of 33 rams with subclinical infection and none o ...1979551360
a comparison of methods for the serological diagnosis of brucella ovis infection. 1977608087
[experimental infection of goats with brucella ovis. bacteriological serological and histopathological studies].the susceptibility of adult male goats to brucella ovis infection was studied. fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of b. ovis strain recently isolated from a case of ram epididymitis. five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. b. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. the five goats sacr ...1977614651
[ultrastructural changes in the testis and epididymis of rams with infectious epididymitis].electron microscope studies were carried out on the ultrastructural changes in the testis and the epidiymis of rams affected with infectious epididymitis, manifesting a pronounced clinical picture. the following conclusions were drawn: 1. the changes in the seminiferous tubules are characterized by a strongly expressed sensitivity of the cells at all levels. noticeable are disturbances in the normal structure of the nuclear substance of both spermatogonia and spermatocytes, vacuolation of the nu ...1978741645
testicular pathology of merino rams.the scrotal contents of 2,281 merino rams of wide age range examined at 3 abattoirs in perth, western australia, showed a 40% prevalence of rams with 1 or more gross lesions. the percentage prevalence of gross lesions found on autopsy were: adhesions 21, testicular atrophy/hypoplasia 14, testicular calcification 13, congenital cyst of the epididymis 6, cryptorchidism 4, chronic epididymitis/scrotal abscesses 2, varicocoele 2, testicular aplasia less than 1, seminoma less than 1. the prevalence o ...1978743044
serological comparison between histophilus ovis, actinobacillus seminis and brucella ovis.the serological relationships between 4 strains of histophilus ovis, the neotype strain of actinobacillus seminis and brucella ovis were examined using a cross-absorption complement-fixation technique. it was found that the 4 strains of h. ovis were serologically homologous and that an incomplete relationship existed between these organisms and a. seminis. anteriserums prepared against one strain of h. ovis and the strain of a. seminis gave a weak, apparently non-specific cross-reaction with br. ...1978743054
[experiments with vaccination against infectious epididymitis in rams].two killed vaccines were produced, differing by the adjuvant used--a hydroxide one and an oil one--employing four strains of brucella ovis. they were applied twice at an interval of 20 days to rams under experimental conditions as well as to rams in filed conditions. on the 45th day following vaccination the experimental rams were challenged with a virulent brucella ovis strain. to determine the acquired resistance the rams were investigated after the complement-fixation test, hemagglutination r ...1978749327
evolution and taxonomy in the genus brucella: progesterone induction of filterable forms of brucella abortus type 2 with revertant characteristics essentially indistinguishable in vitro from those of brucella organism essentially indistinguishable from brucella ovis, as determined by conventional and manometric techniques of identifying brucella organisms, was derived in vitro from a strain of brucella abortus type 2. the basic mechanism responsible for derivation of this organism was incomplete reversion of the l-forms to their original parental characteristics. the agent used to induce the l-forms was a physiologic concentration of progesterone incorporated in the growth medium.1976816233
[ultrastructural changes in the spermatozoa of sheep infected with brucella ovis].the semen was studied of four rams of the mutton merino, suffolk, "kavkazki merino* breeds and a local crossbred race, affected with infectious epididymitis and having semen deteriorated to a various extent, in order to establish the changes in the ultrastruct of spermatozoa through electron microscopy. the following conclusions were drawn on the basis of the results obtained: 1. the changes caused by brucella ovis infection in such cases are chiefly concentrated in the head of spermatozoa. deta ...1977929971
[comparative diagnostic studies of epididymitis in rams caused by brucella ovis].semen samples taken from 21 rams with pronounced pathologic changes were studied in a complex way to establish the etiology contributing to the deterioration of the semen quality. the complement-fixation test was applied with blood serum and semen plasma along with the microscopic study of the semen for the excretion of brucella ovis organisms and a clinical examination. the following results were obtained: 1. the semen plasma of 90.5 per cent of the rams was positive for brucella ovis. a total ...1976951934
endotoxic activity of rough organisms of brucella species.a rough-specific antigen extracted from the rough species brucella ovis and lipopolysaccharide extracted from smooth brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. acetone-extracted whole cells of b. ovis and of b. canis and of a rough mutant of b; abortus had the same toxicity for mice, but it was not possible to extract endotoxin from b. canis by the methods used.1976971943
characteristics of semen changes during brucella ovis infection in rams.the effect of brucellosis on semen quality in rams was studied by means of artificially infecting five rams with a strain of brucella ovis isolated in kenya. infection resulted in reduced semen quality including reduced total sperm output, poor motility and a high percentage of morphological abnormalities. variation in semen quality both between and within rams appeared to be related to progress of the disease in the epididymis as well as to the distribution and severity of lesions in the reprod ...1976982755
[sperm-autophagocytosis in rams affectes with brucella ovis].studied was micrographically the semen of rams affected with infectious epididymitis admixed with leukocytes and brucella ovis organisms. the animals were cft-positive for brucellosis. it was found that in addition to the abundantly phagocytized brucellae in the cytoplasm of the polymorphonuclear leukocytes there were fairly often surrounding sporadic spermatozoa or groups of them whose flagellae and heads have penetrated into the respective leukocyte. to elucidate this phenomenon ultrasections ...19761014362
letter: brucella ovis vaccination of rams. 19751060006
duration of brucella ovis infection in ewes.twenty romney marsh ewes, some 5 to 40 days pregnant, were inoculated with a strain of brucella ovis onto the scarified mucous membrane of the vagina. establish of infection was ascertained by recovery of the organisms from blood; supramammary and inguinal lymph nodes; the vagina, after initial disappearance of the organism from this organ; and the complement fixation tests. the bacteremia was transitory or intermittent, but organisms could not be recovered from blood, or any other tissue examin ...19751126169
studies on h2s production by brucella ovis strains. 19751181906
effectivity of rev 1 vaccine in rams against brucella ovis infection.vaccination with rev 1 at four months of age protected rams against experimental infection with brucella ovis. a double vaccination did not improve immunity whereas rams vaccinated later in life showed a decreased immunity to the infection. lesions and complement fixation reactions were found to be inadequate criteria for the diagnosis of the disease in the individual ram.19751223287
cloning and characterization of the brucella ovis heat shock protein dnak functionally expressed in escherichia coli.the brucella ovis dnak gene, homolog to the eukaryotic hsp70 genes, was cloned by using a drosophila melanogaster probe. comparison of b. ovis and escherichia coli sequences revealed a similar organization for the dnak and dnaj genes and putative regulatory signals. in e. coli transfected with the cloned fragment, b. ovis hsp70 was expressed at 30 and 50 degrees c apparently under the control of its own promoter. the recombinant protein and a b. ovis native protein displaying the same molecular ...19921459952
antimicrobial therapy for rams with brucella ovis infection of the urogenital tract.rams shedding brucella ovis in semen but without palpable abnormalities of the epididymides were treated with long-acting oxytetracycline for 15 days and dihydrostreptomycin for 7 days (n = 9) or conventional oxytetracycline and dihydrostreptomycin (n = 9) for 7 days. nine rams were not treated. more treated rams were considered to have satisfactory breeding soundness examination results at posttreatment weeks 3, 7, 12, and 19. nontreated rams continued to shed b ovis in semen. after treatment, ...19901689288
myofibroblasts and myoepithelial cells in the chicken harderian electron microscopic study of the myoepithelial cells in the chicken harderian gland provides evidence that these cells can be transformed into myofibroblasts. after the application of a brucella ovis suspension in sterile saline onto the eyeball, every 5 minutes for half an hour, myoepithelial cells gradually develop over a 90-minute period the characteristic features of myofibroblasts: bundles of intracytoplasmic microfilament; abundant rough endoplasmic reticulum; prominent golgi complex; ...19911810530
[comparative study of culture media for isolating brucella ovis].for the isolation of brucella ovis, modified thayer-martin medium with the addition of 0.01 micrograms/ml of trimethoprim and 100 iu of nystatin (tmm) was compared with skirrow agar (sk). using viable counting technique, 11 strains were evaluated and the results were compared with those using columbia base agar with bovine blood 7% (cba). ninety-four semen samples of 33 rams from a flock with infection antecedents were cultured on the same media. growth of brucella ovis strains was similar in al ...19911815277
genetic variation at the omp2 porin locus of the brucellae: species-specific markers.the omp2 locus of brucella abortus is composed of two closely related genes (omp2a and omp2b) that encode, and potentially both express, homologous porin proteins. genetic variation at this locus is revealed in the form of restriction-fragment-length polymorphisms which can be used to distinguish the type strains of all six brucella species. five of the six species contain single copies of omp2a and omp2b, whereas brucella ovis appears to have two copies of the omp2a gene. the implications of th ...19901978222
comparison of seroreactivity of rams with brucellosis in a complement fixation test, whole cell elisa and by rams with ovine brucellosis, a high degree of serological correlation exists between the complement fixation (cf) test which utilises antigen extracted from bacteria with hot saline, and the elisa reactivity using methanol-fixed brucella ovis as the assay reagent. since the whole cell elisa (celisa) detects mainly antibodies against surface antigens of b. ovis, it was concluded that the similar findings of the two serological tests is due in part to the presence of membrane antigens in the cf ...19912024447
serological responses of rams to a brucella ovis-vitamin e adjuvant vaccine.rams which were vaccinated at 6-8 months of age with a water-in-oil brucella ovis-vitamin e adjuvant vaccine had significantly higher serum antibody levels than rams vaccinated with a commercial b. ovis bacterin or b. melitensis rev 1. the adjuvant vaccine did not cause abscesses at the site of injection as some water-in-oil emulsions do. two years after vaccination, the vitamin e adjuvant-vaccinated rams had higher antibody level than the other groups. this was most likely due to a secondary re ...19912058270
immunochemical studies on a brucella ovis specific protein antigen.a brucella ovis surface protein antigen (p-ii), obtained by gel filtration with sepharose 4b of a hot saline extract was characterized. the analysis of p-ii over gradient sodium dodecylsulfate electrophoresis yielded an 18.5 and a 20 kda band. in a radioimmunoprecipitation assay using p-ii labeled with 125i, the antigen reacted specifically only with sera from rams experimentally infected with a naturally occurring rough strain of b. ovis and did not react with sera from rams experimentally infe ...19912063544
comparison of subcutaneous and conjunctival routes of rev 1 vaccination for the prophylaxis of brucella ovis infection in rams.the serological response and protection conferred against brucella ovis by the rev 1 vaccine was evaluated in both adult (experiment 1) and young rams (experiment 2) vaccinated either subcutaneously or conjunctivally. in experiment 1 the rev 1 vaccine protected 55.5 per cent and 100 per cent, respectively, of subcutaneously and conjunctivally vaccinated rams against three consecutive challenges that infected 100 per cent of unvaccinated controls. in experiment 2, rev 1 protected 100 per cent of ...19902110377
trials with an enzyme-linked immunosorbent assay (elisa) for the diagnosis of subclinical genital infections in rams caused by histophilus ovis and actinobacillus seminis.the performance of an enzyme-linked immunosorbent assay (elisa) was evaluated in the serological diagnosis of subclinical genital infection in 6 naturally infected ram flocks and 2 experimentally infected ram hoggets. the test employs lipopolysaccharide (lps) antigen prepared by autoclaving from actinobacillus seminis and histophilus ovis. a total of 193 sheep (118 unmated virgin rams and 75 mature breeding rams) were examined clinically, serologically (by elisa) and bacteriologically (semen bac ...19902220189
epididymitis in rams and lambs.this article reviews lamb epididymitis and epididymitis caused by brucella ovis. pathogenesis, occurrence, signs and lesions, diagnosis, prevention, and treatment are discussed.19902245369
polymorphism in brucella spp. due to highly repeated dna.the species of brucella are very closely related, but brucella ovis does not express detectable amounts of a protein, designated bcsp31, that is common to the other species. we studied the lack of expression of bcsp31 by southern analysis. dnas from the b. ovis culture collection strains and field isolates were probed with a 1.3-kb hindiii fragment encoding bcsp31 of brucella abortus. the probe hybridized to a 1.6-kb hindiii fragment of all b. ovis strains tested, showing that the gene is presen ...19902254243
extraction of membrane antigens from brucella ovis and an assessment of their serological activity by immunoblotting.the efficacy and selectivity of chaotropic and phase-partitioning procedures for the extraction of membrane proteins from brucella ovis were compared with a standard sarkosyl method. major group 1, 2 and 3 outer-membrane proteins (omps) of b. ovis stained by coomassie blue in sds-page gels had, respectively, apparent molecular masses of 81/82 kda, 39-41 kda and 30-32 kda. the presence of these bands in the sarkosyl extract of total membrane vesicles (tmvs) indicate that the procedure failed to s ...19902262793
profiles of serological reactivity against cytosoluble antigens of brucella ovis in experimentally infected rams.sera from rams infected with and excreting brucella ovis in the semen (shedders), as well as from animals which had recovered from previous experimental challenge with b. ovis, were analyzed for their serological reactivities against cytosolic antigens of the bacterium. membrane vesicles, including outer and inner membrane components, were precluded from the analyses by subjecting french-pressed bacteria to ultracentrifugation. the resulting cytosolic supernatant was fractionated into four major ...19902279996
antibody response to brucella ovis outer membrane proteins in ovine saline extracts of brucella ovis were composed of vesicles with outer membrane proteins (omps), lipopolysaccharide, and phospholipid as constituents. extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 omps (28,500 apparent molecular weight [28.5k], 27.0k, and 25.5k) represented 81% of the total. group 1 omps and 67.0k, 22.5k to 21.5k, and 19.5k to 18.0k proteins were also detected. adsorption of immune sera wit ...19902298488
brucella ovis excretion in semen of seronegative, clinically normal breeding rams.a commercial flock of suffolk and suffolk-cross breeding rams was monitored for 5 years in an effort to control epididymitis caused by brucella ovis. scrotal palpation, semen evaluations, and vaccination against b ovis were used the first 3 years. serologic evaluation (complement fixation and elisa) was added the fourth year, and bacteriologic culturing was added to the program the fifth year. semen culturing in the fifth year revealed 9 (37.5%) of 24 rams were actively excreting b ovis; 6 of th ...19902298657
brucella ovis epizootic in virgin ram lambs. 19902329082
immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of brucella ovis.brucella ovis rough lipopolysaccharide (r-lps) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. r-lps was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. gel-filtration of the upper phase in a column of bio-gel p-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharide units, 2-keto-deoxy-octulosonic acid (kdo), and monosaccharides. strong acid hydrolysis followed by pap ...19902363245
identification of brucella ovis in formalin-fixed paraffin-embedded genital tissues of naturally infected rams by the indirect peroxidase-antiperoxidase technique. 19882461696
comparison of protein components of different species and strains of brucella.the proteins constituents of brucella abortus, brucella melitensis and brucella ovis were analyzed sds-page. from the comparison appears that the three species of brucella studied shows a different electrophoretic pattern specially at the level of small peptides. on the contrary when two strains of b. abortus are analyzed no differences can be noticed.19892506718
comparison of three serological tests for brucella ovis infection of rams using different antigenic extracts.the sensitivity and specificity of the complement fixation, gel diffusion and elisa tests for the diagnosis of brucella ovis infection of rams have been compared using three different antigenic preparations. the antigens obtained by petroleum ether - chloroform - phenol, or cold saline extractions gave poorer diagnostic results than those obtained by hot saline extraction in all the tests. the best sensitivity was obtained with the elisa (97.6 per cent) followed by the gel diffusion (96.4 per ce ...19892512715
comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of brucella ovis and brucella melitensis.sheep infected with brucella ovis produce antibody responses to the rough lipopolysaccharide and to proteins present in hot saline (hs) extracts of b. ovis (j. i. riezu-boj, i. moriyón, j. m. blasco, c. m. marín, and r. díaz, j. clin. microbiol. 23:938-942, 1986). the distribution and antigenic relatedness of proteins in hs extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting for 41 strains of b. ovis and 26 strai ...19892651307
efficacy of long-acting oxytetracycline alone or in combination with streptomycin for treatment of brucella ovis infection of rams.twenty-four rams inoculated with brucella ovis by conjunctival and preputial routes were treated with a long-acting oxytetracycline alone or in combination with dihydrostreptomycin sulfate. the combined treatment eliminated brucella ovis from 11 of 12 (91.6%) treated rams. only 4 of 12 (33.3%) rams treated with oxytetracycline alone were bacteriologically negative. neither treatment resolved clinical epididymitis in 2 rams affected before treatment. many rams had pathologic lesions in the epidid ...19892712423
discrimination between sheep antibodies to brucella melitensis and to brucella ovis.when preparations containing smooth brucella abortus lipopolysaccharide (lps) were used as antigen in an elisa, strong positive reactions were obtained with sera from sheep infected with brucella melitensis or with brucella ovis. oxidation of the lps with sodium metaperiodate greatly reduced the extent of the cross-reactions with antisera to b. ovis, with little effect on the reactions with antisera to smooth b. melitensis. periodate oxidation of hot saline extract (hsx) antigen of b. ovis marke ...19892800307
temporal elisa response of rams to brucella ovis following experimental infection or vaccination.sera from rams vaccinated with antigens extracted chaotropically from brucella ovis by potassium thiocyanate treatment were used to optimise a whole-cell, enzyme-linked immunosorbent assay (celisa) and to monitor the temporal serological response of rams which had been challenged with infected semen by the intranasal or intrapreputial route. three patterns of celisa response were detected. thirteen of 15 rams intranasally challenged did not respond serologically (pattern 1 or nil response). only ...19892922509
restriction endonuclease analysis of brucella ovis and other brucella species.brucella ovis dna was analysed by using 11 different restriction endonucleases. the most clearly resolved dna fragment patterns were obtained after digestion with the enzyme hind iii. when dna preparations from 35 strains of b. ovis were digested with this enzyme, the fragment patterns appeared to be identical. the patterns obtained after hind iii digestion of dna from one strain each of b. abortus, b. canis and b. melitensis were more similar to each other than to the b. ovis pattern.19852996213
characterization of brucella ovis surface antigens.a rough antigen (sra) extracted from brucella ovis in hot saline by myers procedure, showed three precipitation lines when tested in immunodiffusion against sera from experimentally infected rams. the components responsible for the lines could be isolated by ultracentrifugation or gel filtration which gave 3 fractions, named pi, pii and piii. the lipopolysaccharide (lps) appeared in the pellet (sra-pp) after ultracentrifugation as judged by the presence of lipids, sugar composition, 2 keto-2deox ...19883148241
[brucella ovis as a possible cause of epididymitis in angora goat rams]. 19883210211
serodiagnosis of histophilus ovis-associated epididymitis in elisa for the detection of antibodies to histophilus ovis was used to evaluate the association of epididymal lesions in rams with serologic response to his ovis. comparison of elisa results for his ovis in groups of rams with epididymal lesions with elisa results of clinically normal rams (control group) revealed a significant difference (p less than 0.01) between the control group and those rams from which his ovis was isolated. a significant difference (p less than 0.01) was noticed between ...19883348531
immunoglobulins and immunoglobulin-containing cells in the reproductive tracts of rams naturally infected with brucella ovis.thirteen rams with serological evidence of brucella ovis exposure (cft of 1:8 or greater), but with no or only mild epididymitis, were selected from a ram flock. serum, semen, preputial washings and fluids from the accessory sex glands (asgf) and testis and epididymis (tef) were examined and immunoglobulin (ig) concentrations estimated. genital tissues were examined histologically and the percentages of class specific immunoglobulin containing cells (icc) determined. eleven of these rams had his ...19883355449
[discovery of rams shedding brucella ovis by immunochemical study of the seminal plasma].clinical, bacteriological, serum and immunochemical experiments were carried out on rams, infected with br. ovis. the most advisable for the discovery of infectious rams proved to be the immunochemical research on the seminal plasma. the demonstration of immunoglobulin fractions in the seminal plasma of rams of the infected group permits to find out the rams, detaching br. ovis, without treating the animals with the abortive form of the infection.19873450075
etiologic significance of bacterial isolates from rams with palpable epididymitis.identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ...19863521407
diagnostic sensitivity and specificity of an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection in enzyme-linked immunosorbent assay (elisa) for antibody to brucella ovis was compared with a standard complement fixation test. sera of 176 rams from uninfected flocks gave 175 negative and one suspect elisa reaction (diagnostic specificity 99.4%) whereas the complement fixation test yielded 167 negative, seven suspect and two anticomplementary reactions (diagnostic specificity of 96.0%). diagnostic sensitivity was evaluated on sera of 79 rams from which b. ovis had been isolated. the elisa sh ...19873567755
economics of brucella ovis control in sheep: epidemiologic simulation model.the epidemiology and economics of brucella ovis control in a hypothetical, commercial sheep flock (100 rams and 2,500 ewes) were investigated. the investigation consisted of an epidemiologic simulation model, reported here, and a decision-tree analysis, reported in a companion paper. the epidemiologic model was designed to simulate the transmission and persistence of b ovis in a ram flock during the mating season as well as the nonmating (isolation) season. a constant contact rate was selected f ...19873570957
economics of brucella ovis control in sheep: computerized decision-tree analysis.the epidemiology and economics of brucella ovis control in a hypothetical, commercial sheep flock (100 rams and 2,500 ewes) were investigated. the investigation consisted of an epidemiologic simulation model, reported in a companion paper, and a decision-tree analysis, reported here. it was predicted from the simulation model that b ovis could be eradicated successfully in 2 test periods (less than 1 year) from a flock by using intensive screening and culling. a computerized decision-tree progra ...19873570958
the distribution of histopathological lesions in rams reacting in a complement fixation test for brucella ovis. 19873619789
immunization with brucella melitensis rev 1 against brucella ovis infection of rams.the efficacy of brucella melitensis rev 1 vaccine (rev 1) for the prophylaxis of brucella ovis ram epididymitis was evaluated. twenty-nine 3-month-old rams were vaccinated with 2 x 10(9) rev 1 and 14 were revaccinated with 5 x 10(8) at 14 months of age. six rams remained unvaccinated as a control group. all rams were challenged with 5 x 10(8) b. ovis at 21 months of age. before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams deve ...19873686824
pathology of the accessory sex glands of rams infected with brucella ovis. 19873689264
isolation and antigenic reactivity of brucella ovis outer membrane proteins.brucella ovis cell membranes were isolated from fractured and lysozyme-treated cells by ultracentrifugation. these preparations appeared to consist largely of outer membranes, as judged from the results of ultracentrifugation experiments in sucrose density gradients under conditions that are widely used to separate inner and outer membranes of gram-negative bacteria. the sequential detergent extraction of cell membranes yielded mainly lipopolysaccharide and three groups of outer membrane protein ...19873693544
comparison of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection.brucella ovis hot saline extracts and petroleum ether-chloroform-phenol lipopolysaccharide were compared in an enzyme-linked immunosorbent assay for the diagnosis of b. ovis ram epididymitis. hot saline extracts detected greater numbers of infected rams. chemical characterization of the antigens showed that, although both contained lipopolysaccharide, hot saline extracts also contained outer membrane proteins. these proteins were active as antigens in western blot tests with sera of infected ram ...19863711279
the influence of supplemental vitamins a and e on ovine humoral immune response.these experiments determined if supplemental vitamins a and/or e would enhance ovine antibody responses. all-rac-alpha-tocopheryl acetate was fed to lambs approximately 6 months old (30 to 40 kg) at levels of 33 (controls), 121, 276, 396, and 476 mg/kg of feed (which are total vitamin e levels). primary and secondary immunizations with 10 mg keyhole limpet hemocyanin (klh) were given. a nonlinear dose response of serum antibody titers was observed and the 476 mg vitamin e/kg treatment significan ...19863714722
correlation of the presence of seminal white blood cells and the prevalence of separated spermatozoal heads with subclinical brucella ovis infection in rams.a breeding soundness evaluation was conducted on 824 colorado range rams. these rams were determined to be free from epididymitis via testicular palpation. semen evaluation included microscopic observation for the presence of wbc. of the 824 rams, 15.5% failed the breeding soundness evaluation on the basis of the semen evaluation: 10.6% had wbc in the semen and 4.9% had poor sperm morphology. the prevalence of brucella ovis isolation varied from 0% to 16.2% within flocks. the prevalence of subcl ...19863733506
demonstration of a peptidoglycan-linked lipoprotein and characterization of its trypsin fragment in the outer membrane of brucella spp.the sodium dodecyl sulfate (sds) extraction-trypsin digestion protocol used by braun and sieglin (v. braun and u. sieglin, eur. j. biochem. 13:336-346, 1970) to show the peptidoglycan-linked lipoprotein of escherichia coli was applied to both brucella abortus and e. coli. whereas a single polypeptide of 8,000 molecular weight was obtained from e. coli, several proteins of apparent molecular weight lower than 35,000 were demonstrated by sds-polyacrylamide gel electrophoresis in b. abortus. these ...19863744559
identification of immunoreactive membrane antigens of brucella ovis by elisa profiling.enzyme-linked immunosorbent assay (elisa) profiling was used to identify the immunoreactive membrane antigens of brucella ovis. immunoreactive membrane antigens obtained after detergent extraction of the bacterial membrane complex (inner and outer membranes) were resolved into five peaks (a, b, b1, c and d) by gel permeation chromatography. aliquots from each of the chromatographed fractions were coupled to 96-well microtitre plates and immunoreactive fractions identified with sera from two rams ...19863764093
immune response in rams experimentally infected with brucella ovis.cellular as well as humoral immune responses were detected in six rams experimentally infected with brucella ovis. specific antibodies were detectable by enzyme-linked immunosorbent assay by day 11 after infection in all the rams. the levels of igm antibodies and total antibodies in the serum rose until 33 and 41 days after infection respectively, then levelled off. antigen-induced blastogenic responses by lymphocytes developed as early as five days after infection in all rams but had decreased ...19863764107
examination of rams culled during an ovine brucellosis accredited free flock scheme.during the early stages of an ovine brucellosis accredited free flock scheme in new south wales 62 rams were examined to determine the status of their flocks of origin with regard to infection with brucella ovis. forty rams were selected because they were either single reactors to low titre or one of a small number of reactors in a b. ovis complement fixation test of the whole ram flock. twenty two rams were selected because they had palpable abnormalities within the scrotum but were negative se ...19863778368
serological, bacteriological and pathological changes in rams following different routes of exposure to brucella ovis.mature merino rams were exposed to brucella ovis by contact with infected semen, using either ewe transmission, intrapreputial, intranasal or intrarectal inoculation of infected semen or intrapreputial inoculation of b. ovis culture. thirty-six of the 41 rams developed significant complement fixation (cf) test titres, but only 9 of these reactors showed clinical, bacteriological or pathological evidence of infection. infection occurred in some of the rams from all groups. the results are discuss ...19863800795
a rapid slide agglutination test for the serodiagnosis of brucella canis infection that employs a variant (m-) organism as improved antigen is described for use in the serodiagnosis of canine brucellosis. the novel antigen employs a less mucoid (m-) variant of b. canis. the replacement of brucella ovis with b. canis (m-) cells as antigen in slide agglutination tests would increase accuracy by reducing the rate of false positive results from ca. 50% to ca. 10%. rose bengal stained b. canis (m-) cells are slightly more sensitive than the commercially available brucella ovis as slide test antigens. both test procedu ...19873802828
ovine brucellosis: repeated vaccination with rev 1 vaccine and the prevalence of the disease in the winburg district.the effect of repeated vaccination of ram lambs with brucella melitensis rev 1 vaccine was studied in relation to complement fixation titres. the presence of brucella organisms in semen and changes in neutrophil densities of the semen were also studied. a survey of the incidence of ovine brucellosis in the winburg district on the basis of a clinical examination of rams and the complement fixation test is also reported on. repeated vaccination of ram lambs with rev 1 vaccine resulted in high comp ...19853836306
evaluation of an enzyme linked-immunosorbent assay for the diagnosis of brucella ovis infection in rams.a simple enzyme linked immunosorbent assay (elisa) was developed for the serological diagnosis of brucella ovis infections in rams. serums from brucellosis accredited-free flocks and flocks known to be infected with b. ovis were tested and the results correlated with warm complement fixation (cf) test and bacteriological examination of semen. both the elisa and the cf test detected 0.5% false positive reactions in rams from clinically negative flocks. however the elisa detected significantly mor ...19853893408
association of age of ram with distribution of epididymal lesions and etiologic agent.a 2-year study of the frequency of isolation of various organisms from mature and yearling rams with epididymitis was conducted at the us sheep experiment station at dubois, idaho. investigation into the distribution of lesions in the epididymis in relation to age of the ram also was studied. serologic or bacteriologic evidence of brucella ovis infection was demonstrated in 79.5% of the mature rams with epididymal lesions. actinobacillus seminis and histophilus ovis were the 2 most frequently is ...19863949615
distribution of brucella ovis in the tissues of rams reacting in a complement fixation test for ovine brucellosis. 19863954693
detection of antibody in rams with contagious epididymitis, using the enzyme-linked immunosorbent enzyme-linked immunosorbent assay, using whole-cell and sonicated antigens prepared from brucella ovis, actinobacillus seminis, and actinobacillus seminis-like cultures isolated from rams in wyoming, was able to detect antibody to these antigens in rams with epididymitis. the whole-cell antigens used in this procedure gave lower background values, compared with those of the sonicated antigens. the procedure was able to detect antibody in rams before clinical signs of epididymitis became appar ...19863963577
use of phage for the identification of brucella canis and brucella ovis cultures.the brucella phage strains r, r/o and r/c standardised at routine test dilution on their propagating strains were effective in identifying cultures of brucella ovis and b canis and in differentiating these from other non-smooth brucella isolates.19853975479
[comparative studies of the properties of br. ovis strains 03, 303 and 100671 to those of local strains for the production of antigen].colorimetric analysis, biochemical methods, and the complement-fixation test were employed to study 3 australian and 5 bulgarian strains of brucella ovis. determined were some biochemical manifestations, the adaptation capacity with regard to aerobic conditions of cultivation without serum, the amount of polysaccharides contained in the strains, and the properties of the antigens obtained as checked via cft. milanov's method was employed for antigen production. three of the bulgarian strains sho ...19853992937
prevalence and causes of ram epididymitis in utah.palpation of the testicles and epididymides was used to determine the prevalence of ram epididymitis (re) in utah range flocks over a 5-year period. of 62 range flocks examined, 58 had rams with palpable lesions. a comparison was made of prevalence by right vs left testicle involvement, but no significant difference was found. culling all rams with palpable lesions from the flock progressively reduced the annual incidence, but did not eliminate re from any flock. semen and specimens of reproduct ...19853997640
use of enzyme-linked immunosorbent assay for detection of antibodies to brucella ovis in sheep: field enzyme-linked immunosorbent assay (elisa), using an autoclave-extracted soluble antigen, for the detection of serum antibodies to brucella ovis in sheep was developed. the test seemed to be both sensitive and specific, on the basis of the control groups studied. the antigen showed no deterioration in prepared plates stored at -70 c for up to a year. the elisa was used in conjunction with palpation of rams for epididymal lesions as a means to detect and control b ovis infection in a naturally ...19854037490
susceptibility of male white-tailed deer (odocoileus virginianus) to brucella ovis infection.infection with brucella ovis was established by conjunctival instillation in 8 male white-tailed deer (odocoileus virginianus). infection was transient in young bucks, but persisted in bucks that were mature when inoculated. the deer were euthanatized and necropsied at various intervals after inoculation. brucella ovis was recovered from a mature buck at necropsy on postinoculation day 429. four deer had gross lesions and histopathologic changes of the epididymides. a mature noninfected buck con ...19854037505
experimental infection of goats with brucella ovis.six goats were inoculated with brucella ovis. two goats were inoculated with infected semen by the intratesticular route and 2 each by installation of the semen on to the nasal or preputial epithelium. all goats produced antibody responses as measured by an enzyme-linked immunosorbent assay (elisa) procedure and the serums of 5 goats reacted in complement fixation tests for b. ovis. the 2 goats inoculated by the intratesticular route and one receiving b. ovis instilled intranasally subsequently ...19854062738
antibody responses to brucella ovis in maternal and fetal sheep. 19694181004
field evaluation of the gel diffusion test for the diagnosis of ram epididymitis caused by brucella ovis.a comparison was made with the microslide gel diffusion technique, the complement fixation test, cultural isolation, and clinical examination in detecting ram epididymitis caused by brucella ovis. the results of the gel diffusion method are shown to be similar to those obtained by the complement fixation test and isolation of b. ovis from cultures of semen. the technique offers a reliable diagnostic method adaptable to field use in controlling ovine brucellosis and is more practical.19734203332
brucella ovis as a cause of abortion in ewes. 19744208504
comparative sensitivity of gel-diffusion and tube agglutination tests for the detection of brucella canis antibodies in experimentally infected dogs.the microslide gel-diffusion and macro-tube agglutination techniques to detect brucella canis antibodies in dogs were compared. sera from dogs experimentally infected with b. canis and a random sample of dog sera with unknown histories of exposure to this organism were examined. the results of the gel-diffusion method employing specific rough brucella saline-extract antigens of b. canis and brucella ovis were comparable to those obtained by the tube agglutination test. the easily prepared, stabl ...19744210726
the incidence of brucella ovis infection in south african rams: a serological survey. 19724217833
[characteristics of the so-called "brucella ovis" (new zealand)]. 19724402417
an automated complement fixation test for brucella ovis in sheep. 19744524883
the complement fixation test for the diagnosis of brucella ovis infection in sheep. 19744531600
studies of antigens for complement fixation and gel diffusion tests in the diagnosis of infections caused by brucella ovis and other brucella.sonically treated and saline-extracted antigens of brucella ovis, b. canis, b. abortus, and b. melitensis were compared in gel diffusion, complement fixation, and serum absorption tests. all the sonically extracted antigens showed cross-reactions with sera from animals infected or immunized with these species, whereas the saline-extracted antigens were specific for the surface of the rough or smooth colonial phase of the species or strain. the saline-extracted antigens of b. ovis and b. melitens ...19724624210
[sero-epidemical study of the hazards of occupational infections in the course of contact with brucella ovis (epizootic foci, laboratories)]. 19734732970
studies on the transmission of brucella ovis infection in rams. 19734734475
pathogenesis of epididymitis caused by brucella ovis in laboratory animals. 19734755775
[dynamics of igm and igg in rams experimentally infected with brucella ovis]. 19734799562
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