| identification of immunoglobulins associated with complement fixation, agglutination, and low ph buffered antigen tests for brucellosis. | serums from infected cattle, cattle with persistent postvaccinal antibody, and serologically "positive" noninfected cattle were fractionated into major immunoglobulin classes by diethylaminoethyl (deae)-cellulose chromatography and by sucrose density gradient centrifugation. each fraction was assayed for anti-brucella activity by standard tube-agglutination test (stt), buffered tube-agglutination test (btt), and complement-fixation test (cf). in the serums from experimentally infected cattle, an ... | 1976 | 3997 |
| cellular immunity against bacteria (intracellular and extracellular parasites) in experimentally aged rats (author's transl). | the authors study the activity of res in rats with progeria-like syndrome of selye, at the occasion of a repeated infection with bacteria--intracellular parasites (brucella abortus 19) and bacteria--extracellular parasites (diplococcus pneumoniae). they establish that immunization improves the activity of res of the experimentally aged rats; still, its phagocytic and digestive functions remain by far feebler than those of the rats unsubjected to experimental ageing. by the aged animals the res i ... | 1976 | 8877 |
| resistance against intra- and extracellular bacteria in experimentally aged rats (author's transl). | the authors study the cellular activity of res against the microbes intracellular and extracellular parasites in rats with pronounced progeria-like syndrome of selye. res of the experimentally aged animals copes, through with a certain delay, with the invading extracellular bacterium diplococcus pneumoniae, but is utterly helpless against the intracellular microbe br. abortus 19. these relations are completly inverted in the rats that have not been experimentally aged. | 1976 | 8878 |
| public health importance of buffaloes and cows udder tissues. | | 1976 | 8937 |
| penicillin resistance and penicillinase production in clinical isolates of bacteroides melaninogenicus. | the minimum inhibitory concentrations (mic) of penicillin and six other antimicrobials were determined for 50 clinical isolates of bacteroides melaninogenicus. agar dilution susceptibilities were performed using supplemented brucella blood agar and the proposed national committee for clinical laboratory standards standard method for anaerobes; results with the two methods were comparable. a penicillin concentration >/=0.8 mug/ml was needed to inhibit 56% of the isolates, whereas 100% were suscep ... | 1977 | 16564 |
| tumor growth in the absence of circulating antibodies. | | 1977 | 17034 |
| activity of spectinomycin against anaerobes. | the in vitro inhibitory activity of spectinomycin was tested against various anaerobic bacteria. different results were obtained with different media and with different initial ph's of the media. the highest minimum inhibitory concentrations for bacteroides fragilis (formula: see text 128 mug/ml) were obtained with the use of wilkins-chalgren agar (ph 7.2) and brucella blood agar (ph 7.0). brucella blood agar at higher ph's (7.4 and 8.0) and mueller-hinton and diagnostic sensitivity test agars p ... | 1977 | 18985 |
| cholinesterase activity of sperm from rams infected with brucella ovis. | studied were the indices of cholinesterase activity of semen taken from 23 normal rams and 23 rams infected with brucella ovis, the latter being positive by the complement-fixation test and showing a varying deterioration of the semen production. the results obtained were processed biometrically. established were dependable differences. the cholinesterase activity of semen of brucellosis-affected rams proved four times higher than that of normal rams' semen: 39.45 +/- 5.49 microleter to2 for 1 h ... | 1977 | 22157 |
| changes in affinity of 19 and 7s antibodies at the cellular level in responses to hapten conjugates of varying t dependency. | | 1978 | 23217 |
| development of a rose bengal stained plate-test antigen for the rapid diagnosis of brucella canis infection. | | 1978 | 30592 |
| [effect of physical and chemical factors on the antigenic activity of the cell walls of brucella abortus 99]. | studied was the effect of some physical and chemical factors--heating, acidity, treatment with ultraviolet rays, and enzyme treatment--on the activity of the brucella abortus 99 cell wall antigen. the activity of the antigen was determined through the microreaction of complement-fixing after kolmer. it was found that the antigen was most sensitive to acid treatment and treatment with ultraviolet rays, and was more slightly sensitive to the effect of alkaline agents and pronase. besides, the anti ... | 1978 | 33483 |
| evaluation of blood culture procedures in a pediatric hospital. | to determine optimal clinical laboratory techniques for detecting pediatric bacteremia, we studied 7,768 consecutive blood cultures in a 1-year period. blood was inoculated into one vented 50-ml bottle of brucella broth with 0.05% sodium polyanetholsulfonate and one unvented 50-ml bottle of columbia broth with 0.05% sodium polyanetholsulfonate and 0.05% cysteine. bottles were visually examined for growth on days 1 through 7 and blindly subcultured aerobically and anaerobically on days 1, 2, and ... | 1979 | 34623 |
| properties of the berkeley phage lytic for "brucella melitensis" and other species. | | 1978 | 37809 |
| maturation of bursal stem cells within allogeneic or syngeneic bursal microenvironment [proceedings]. | | 1979 | 43807 |
| intraembryonic mesenchyme as a source of lymphoid stem cells [proceedings]. | | 1979 | 43808 |
| studies on antibody levels to brucella abortus, toxoplasma gondii and leptospira serogroups in sera collected by the national serum bank during 1974-1976. | the new zealand national serum bank is a collection of human sera consisting predominantly of specimens taken from healthy new zealand blood donors. the studies presented here were designed to assess the antibody levels in two urban centres to brucella abortus, toxoplasma gondii and several leptospiral serogroups including all those found in new zealand. in none of the sera could complement fixing leptospiral antibodies be detected. there was evidence of low level immunity to both toxoplasma gon ... | 1979 | 44946 |
| effect of ochratoxin and aflatoxin on serum proteins, complement activity, and antibody production to brucella abortus in guinea pigs. | the effect of ochratoxin alone and in combination with aflatoxin and brucella abortus antigen on complement activity, serum proteins, and antibody response in guinea pigs was investigated. ochratoxin did not affect complement activity or antibody response and there was no interaction between ochratoxin and aflatoxin on any of the responses tested. ochratoxin significantly lowered the level of beta-globulin in serum of guinea pigs. there was no significant interaction between aflatoxin and antige ... | 1975 | 45955 |
| taxonomic distribution of the antigen eliciting bactericidal antibody for bordetella pertussis. | strains of bordetella pertussis varied in their ability to elicit (in mice) an antibody bactericidal for an antiserum-sensitive strain of b. pertussis, although antibody was usually detectable after only one injection. high titres were produced by a course of seven injections with all strains of b. pertussis tested (six of phase i and three of phase iv) but not with three strains of other bordetella species nor with two unrelated organisms, a finding of possible taxonomic value. preliminary inve ... | 1975 | 49392 |
| [inhibition of antigenic competition by immunostimulants]. | the diminution of immune response against srbc induced in mice, by a prior injection of hrbc was counteracted by addition of certain immunostimulants to srbc. the intensity of inhibition of antigenic competition was related to the quantity of immunostimulant added to srbc. some immunostimulants (b. abortus, lipopolysaccharide) were more active than others (c. parvum, poly i : c). to inhibit antigenic competition immunostimulant had to be injected after or in mixture with srbc never before. | 1975 | 56240 |
| shared antigens between bacteria and guinea pig line 10 hepatocarcinoma cells. | this study was undertaken to investigate the possibility that listeria monocytogenes, brucella abortus, and salmonella typhimurium share antigenic components with guinea pig line 10 hepatocarcinoma cells. rabbits were immunized with sonicates of these bacteria or line 10 tumor cells. other rabbits were immunized with line 1 cells, a tumor with antigenic characteristics different from those of line 10. the binding of antibodies to radiolabeled antigens prepared from extracts of bacteria and line ... | 1976 | 57823 |
| shared antigens between human malignant melanoma cells and mycobacterium bovis (bcg). | this study was undertaken to investigate the antigenic relationships between human malignant melanoma cells and mycobacterium bovis (bcg). rabbits were immunized with sonicates of bcg or with malignant melanoma cells from different patients and the resulting antisera were tested for their capacity to bind radiolabeled soluble extracts prepared from bcg and melanoma cells. the binding of antibodies to radiolabeled antigens was studied by precipitation of radiolabeled antigen-antibody complexes by ... | 1976 | 58033 |
| biochemical basis of the serological cross-reactions between brucella abortus and yersinia enterocolitica serotype o:9. | a method based on the inhibition of agglutination is described that may be used for the differential serological diagnosis between b. abortus and y. enterocolitica serotype o:9. an antigen with high immunological capacity was isolated from brucella. this antigen inhibited both homologous and heterologous agglutination by brucella antiserum, but only the heterologous agglutination by yersinia antiserum. it proved to be constitued of a polysaccharide (n-acetylglucosamine, glucose, mannose and 2-ke ... | 1975 | 59566 |
| [antigenic analysis and immunological studies of the uterotropic bacterial strains sh6 and 01 isolated from aborted cows]. | the uterotropic strains sh6 and o1 were studied immunoelectrophoretically to establish their antigenic relationship. the more readily diffusing component of the o-antigenic fraction of the strains proved immunologically identical with lpa of the r brucella organisms, while the slower component showed common determinants with the o-antigenic fraction of yersinia pseudotuberculosis. immunodiffusion revealed that the o-antigenic fraction of the o1 strain and lpa of the s brucella organisms contain ... | 1976 | 60821 |
| [immunocytogenesis and formation of antibodies in experimentally aged animals (author's transl)]. | the authors use white rats with experimentally induced progeria-like syndrome of selye. the immunologic response following inoculation with the extracellular microbe-parasite d, pneumoniae and the intracellular parasite br. abortus 19 is studied serologically, cellularly and biochemically. the experimentally aged rats show diminished and retarded plasmocyte reaction and weaker and slower agglutinine synthesis as compared with the controls. the immunochemical analysis of the serums of the experim ... | 1976 | 60831 |
| [antigenic and serologic properties of yersinia enterocolitica]. | | 1976 | 62475 |
| brucellosis, immunodepression, and levamisole. | | 1977 | 64900 |
| [bacterial infection in cows associated with abortion, endometritis and sterility]. | isolated were two types of bacteria (having common morphologic and cultural behaviour and related antigenic composition) from aborted fetuses and placentae of cows with a positive reaction after both huddleson and placentae of cows with a positive reaction afterboth huddleson and wright. they were shown to be new species of the group mimeae and the genus yersinia. it was demonstrated through immunodiffusion and immunoelectrophoresis that these new bacteria present antigenic relationship with bru ... | 1976 | 65045 |
| radioimmunoassay of igm, igg, and iga brucella antibodies. | a radioimmunoassay (r.i.a.) has been devised to measure the serum antibody against brucella abortus in each of the immunoglobulin classes igm, igg, and iga. this test was applied to 46 sera from individuals with various clinical types of brucellosis, and the results were compared with the results of conventional direct and indirect agglutination and complement-fixation tests. the r.i.a. provided a highly sensitive primary-type assay which avoided the difficulties with blocking or non-agglutinati ... | 1977 | 68181 |
| isolation of a phage lytic for several brucella species following propagation of tbilisi phage in the presence of mitomycin c. | a new brucella-phage, designated d, was isolated from a batch of tbilisi phage grown on brucella abortus strain 544 in the presence of mitomycin c. although resembling tbilisi phage in morphology, it differed in its host range, adsorption properties, chemical stability and antigenic specificity. | 1977 | 70199 |
| influence of the antigenicity of brucella preparations on modulation of the immune response to sheep erythrocytes. | the relationship between the expression of surface antigenicity of inactivated brucella and the immunostimulant properties on the sheep erythrocyte response was studied in mice. the agglutinogenic brucella abortus b19s preparation was compared to two non-agglutinogenic preparations (b19r and pb), using the plaque-forming and rosette-forming cell tests. when brucella and antigen were injected together, only non-agglutinogenic preparations, even used at low doses, were able to increase the plaque- ... | 1978 | 78896 |
| some properties of interferons induced by stimulants of t and b lymphocytes. | murine interferons induced by viruses and by non viral substances such as lectins, tilorone, lipopolysaccharide and brucella have been analysed by affinity chromatography on a column of sepharose coupled to anti-bodies directed against viral interferon. b cell stimulants induced interferons which are antigenically related to viral interferon and t cell dependent mitogens induced an interferon species which appears to be antigenically and physicochemically different. despite their antigenic diffe ... | 1977 | 80830 |
| [biochemical study of serum from sheep infected with brucella ovis]. | blood serum was used of 49 rams and 20 ewew infected with brucell ovis. the study on the total protein and the protein fractions of rams revealed that there existed gamma-globulinemia, the percent of the gamma-globulin rise showing a positive correlation with the morphologic changes in the testes. the alfa-globulins were found to rise immediately following the experimental infecting of the sheep for about a month, after which they came back to normal. in experimental infection the activity of th ... | 1978 | 83049 |
| [t- and b-lymphocytes and an analysis of the specific reactivity in experimental brucellosis]. | there was an increase of a relative and absolute t-lymphocytes content and a considerable reduction of b-lymphocytes in the peripheral blood of guinea pigs infected with a high doses of brucella of vaccine strain 19-ba. there proved to be no correlation between the number of t-lymphocytes in the blood and the intensity of burnet's test. the content of b-lymphocytes was compared with different antituberculosis antibodies in the blood serum of the infected animals. | 1979 | 84458 |
| [aspects related to the antigenic structure and serological specificity of brucellae phylogenetically related to s- and r-forms and dissociated into r-variants]. | the antigenic structures and serological properties of brucellae s- and r-forms, phylogenetically differentiated, and dissociated r-variants are studied by means of gel-precipitation, immunoelectrophoresis and fixation of the complement. the brucella suis 1330s, brucella suis 1330r, brucella ovis 02 and brucella abortus 99 are involved in the experiments. both specific and general antigenic structures are established in all strains studied, but only homologous antibodies from the antigen-antibod ... | 1978 | 88791 |
| [method of isolating brucellae from field rodents]. | | 1979 | 90209 |
| [antigenic structure of the l forms of br. abortus 544]. | the gel precipitation test after ouchterlony and mancini was employed to study comparatively the antigenic structures of s and l forms of brucella abortus 544. reduction of the antigenic fractions was established with the l-transformed brucella organisms as compared to the initial cultures. reduced were mainly fractions of the antigenic structures that took part in cell wall building. cytoplasmic antigens remained unchanged. those that participated in the building of cell walls precipitated in c ... | 1979 | 92852 |
| differentiation of antibodies against brucella abortus and yersinia enterocolitica by enzyme-linked immunosorbent assay. | the enzyme-linked immunosorbent assay (elisa) was found to be from ten to hundred-fold more sensitive than commonly used tube agglutination text. the elisa using both direct and indirect inhibition assays revealed antigenic differences between brucella abortus and yersinia enterocolitica o-group v (serotype 9), which are undetected by commonly used serological routine tests. the results point to the possibility that the elisa assay can be used as a serological method for differentiation between ... | 1979 | 94003 |
| ia antigens in serum during different murine infections. | there exists in the mouse a family of i-region-controlled (ia) antigens which carry carbohydrate-defined determinants. these antigens appear in serum as glycolipids and seem to be actively secreted by antigen-activated t-cells. this paper describes the ability of selected viral, bacterial, and protozoal infections of mice to markedly alter the serum levels of these ia antigens. all the infectious agents examined induced substantial augmentation or suppression of serum ia concentrations or both. ... | 1979 | 94905 |
| actinobacillus seminis infection in sheep in the republic of south africa. iii. growth and cultural characteristics of a. seminis. | bacteriological tests were done on a large number of different strains of actinobacillus seminis and also, repeatedly, on the same culture or on different cultures taken periodically from the same donor animal. these tests were also applied to strains of a. seminis representing different serological types, which in turn were compared with strains of brucella ovis. the tests as applied proved that a. seminis strains have defined, morphological, staining, cultural and biochemical properties, altho ... | 1979 | 95214 |
| b-cell tolerance. ii. trinitrophenyl human gamma globulin-induced tolerance in adult and neonatal murine b cells responsive to thymus-dependent and independent forms of the same hapten. | neonatal splenic b cells which are responsive to thymus-dependent antigens (td) are exquisitely susceptible to induction of tolerance (1,2). this state of tolerance is not mediated by suppressor t cells and is not a result of suboptimal macrophage function (1 and footnote one). in adult mice, induction of b-cell tolerance is not achieved with doses of antigen 1,000-fold higher (1) than those required to produce the same degree of unresponsiveness in neonates. in contrast to these results, studie ... | 1977 | 95788 |
| protection of mice against haemoprotozoan babesia microti with brucella abortus strain 19. | when brucella abortus strain 19 is given intraperitoneally to mice it protects them against subsequent infection with large doses of babesia microti. the protection obtained was more effective when b. abortus was given intraperitoneally than when it was injected subcutaneously. this non-specific protection seems to be best explained by the stimulation of macrophages so as to release a mediator which limits the intracellular replication of the parasites. | 1978 | 95914 |
| further studies on the h-2 linked dependence of the adjuvant action of brucella abortus. | the b 19s strain of brucella abortus is found to act as an adjuvant to the anti-sheep red blood cell (srbc) reaction in some congenic strains of mice but not in others. if the recipient is h-2b, there is no adjuvant effect (b 19s); neither is there (thymus-dependent anti-b 19s reaction as measured by thymocyte activation and change of electrophoretic mobility. in contrast, there is a thymus-independent anti-b 19s reaction (production of haemagglutinins) as good in the h-2b mice as in the others. ... | 1978 | 96008 |
| [observation on the antigenic affinity of leptospira and brucella in the serological examination of cattle]. | 36,9% bovine serums which showed a non-specific reaction in examination for brucellosis had titre greater than or equal to 1:400 leptospirosis agglutinins. 15.1% of the serums showed a positive reaction (greater than 1:800), mostly those against l. sejroe and l. grippotyphosa. the cross reactions, found in individual cases within up to 39 days since the first reaction to brucellosis in cattle may signalize early leptospira infections. | 1978 | 96568 |
| [physico-chemical properties of microbial antigens and the immuno-allergic tests]. | experiments were conducted on guinea pigs sensitized by the delayed and immediate types of allergy. different antigens obtained from the strain of brucella abortus ba-19 were used for sensitization and the resolving action. comparison of the resolving properties of the corpuscular, soluble (ultrasound treated) antigens and purified protein fractions, polysaccharide and rna was carried out in the skin reactions of the immediate and delayed type, passive skin anaphylaxis, acute anaphylactic shock, ... | 1978 | 96888 |
| international standard for anti-brucella abortus serum: comparison of the complement-fixing activity of the first and second international standards and the eec standard. | although the three standards have similar agglutinating activities, it was found that the first international standard had about half the complement-fixing activity of the second international standard and the eec standard. the complement-fixing activities of the latter two standards were similar. on the basis of these results, showing that the agglutination and complement-fixation tests measure different biological activities in anti-brucella abortus sera, the who expert committee on biological ... | 1978 | 96951 |
| whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune responses in bovine brucellosis. | a study was conducted to develop an in vitro whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune response in bovine brucellosis. a soluble antigen (basa) prepared from killed cells of brucella abortus 1119-3 was used. cattle infected with b. abortus field strains, b. abortus 19 calfhood- and adult-vaccinated cattle, and nonexposed cattle were tested. blood was diluted 10-fold in rpmi-1640 medium (without added serum) and cultured with basa (at a concentration of 2.2 ... | 1978 | 97304 |
| maternal vitamin e alters passively acquired immunity of chicks. | passively transferred antibody levels were significantly increased in plasma of two- and seven-day-old chicks when the dams were fed 150 and 450 p.p.m. vitamin e prior to immunization with brucella abortus. however, if the hens were fed 90, 300 and 900 p.p.m. vitamin e before immunization, the chicks evidenced no increase in antibody titers relative to controls. this nonlinear antibody response by the reticulo-endothelial system to vitamin e confirms in gallus domesticus similar results reported ... | 1978 | 97647 |
| [bovine brucellosis in a village of lower-casamance (sénégal). i. serological and bacteriological diagnosis]. | | 1977 | 97737 |
| a comparison of three serological tests in the diagnosis of caprine brucellosis. | the serum agglutination test (sat), the rose bengal plate test (rbpt) and the milk ring test (mrt) were used in the diagnosis of caprine brucellosis. there was a close correlation between the sat and rbpt when both tests were negative but the rbpt failed to detect 79.82 per cent of sera in excess of 50 iu. also, owing to the relatively poor milking potential of the nigerian goat and the false positive results with the mrt, it is concluded that the sat offers a better serological diagnostic tool ... | 1978 | 97740 |
| [comparison of the methods for detecting brucella carriers]. | | 1978 | 97853 |
| a microagglutination test for yersinia enterocolitica infection: recognition of multiple serotypes in experiments with stained suspensions of serotype o5,27. | when suspensions of yersinia enterocolitica were stained with triphenyl-tetrazolium chloride (ttc) their antigenic specificity, as determined by tube and microtitre-plate agglutination tests, was altered. thus, a ttc-stained suspension of serotype o5,27 detected antibodies to nine o serotypes of y. enterocolitica in sera from experimentally infected animals but did not cross-react with antisera to organisms of five other unrelated genera including brucella. the same suspension did, however, cros ... | 1978 | 98635 |
| epiphysitis in rams following vaccination against brucella ovis infection. | | 1978 | 98740 |
| [relation of the sex of guinea pigs to sensitivity to brucella abortus infection and their immune response]. | | 1978 | 98812 |
| [antitumor activity of the bacterial walls of br. abortus 99]. | a brief chronologic survey is made of investigations that reveal that the bacterial cell walls and their chemical components exhibit antitumoral activity. discussed is the problem which are the constituents of the bacterial wal and what is the mechanism through which such effects are produced. the conclusion is drawn that some peptides participating in the structure of the bacterial wall after their release in body stimulate the macrophages and thus confer antitumoral activity to them. | 1978 | 98899 |
| [development of allergic reactivity to artemesia pollen during combined sensitization to pollen and microbes]. | some regularities of formation of hypersensitivity of the immediate type to the pollen of artemisia absinthium were studied under conditions of combined hypersensitivity to pollen and brucella abortus 19-ba vaccine strain; the latter was administered 3, 12, and 28 days after the pollen. the degree of specific allergic reconstruction to the pollen was studied by passive skin anaphylaxis after ovary, indirect degranulation of mast cells of healthy rats, and by general anaphylaxis in response to in ... | 1978 | 99195 |
| macrophage involvement in the antitumor activity of brucella abortus ether extract against experimental lung carcinoma metastases. | | 1978 | 99230 |
| the causative organism of contagious equine metritis 1977: proposal for a new species to be known as haemophilus equigenitalis. | the aetiological agent of contagious equine metritis (cem) has been investigated bacteriologically in a wide range of cultural and conventional biochemical tests, in the eletron microscope, for dna base composition (36.1 per cent gc), for susceptibility to various antimicrobial agents and antigenically by means of tube and slide agglutination tests. the organism is a fastidious, gramnegative, non acid-fast coccobacillus which in biochemical tests is very unreactive. in conventional tests, only t ... | 1978 | 99302 |
| t-independent responses in b cell-defective cba/n mice to brucella abortus and to trinitrophenyl (tnp) conjugates of brucella abortus. | cba/n mice have an x-linked immune defect in b lymphocyte function which leads to their inability to respond to several thymus-independent antigens. we report here that these mice and immunologically defective f1 male (cba/n x dba/2n) mice can respond to brucella abortus and to 2,4,6-trinitrophenyl derivatives of brucella abortus (tnp-ba). these responses can be obtained in vivo and in vitro and are thymus-independent by the criteria that (a) they can be transferred to irradiated recipients by b ... | 1978 | 99320 |
| early ontogeny of germinal center formation in the chicken. | germinal center formation was studied in the spleen of young chickens immunized in ovo and at the time of hatching. when immunization was performed on day 18 in ovo and on the day of hatching, the first germinal centers were observed at 4 days. this is markedly earlier than in unimmunized chickens, where the first germinal centers appear at the age of 10 days or later. germinal center formation preceded significant antibody production. the possible role of germinal centers in the generation of i ... | 1978 | 99335 |
| brucellosis in elk. ii. clinical effects and means of transmission as determined through artificial infections. | | 1978 | 99525 |
| [jet method of administration of live brucella vaccine]. | a good survival of brucellae in the organism with the development of a stable and prolonged immunity was noted in vaccination of guinea pigs with live brucella vaccine prepared from the br. abortus strain 19-ba. local reactions were weak, and no general reactions were observed. in using the jet method of vaccination with the injectors bpi-4 and bi-2 on a limited group of persons (194) the local and general untoward response was weak, but in immunological respect this method proved to be effectiv ... | 1978 | 99922 |
| [effect of brucellosis protective antigen on the phagocytic activity of the cells]. | the authors present the results of study of the protective function of cells of the local inflammatory focus caused by the administration of live brucella culture to guinea pigs immunized with a protective antigen in the optimal (0.6) and increased (2 mg) doses. macrophages of guinea pigs vaccinated with the optimal immunized dose of the protective antigen phagocytized brucellae actively; an increased dose of the antigen suppressed the ingestive and the digestive functions of macrophages, this a ... | 1978 | 99925 |
| [redox processes in allergic reactions of the delayed type to microbial antigens]. | the influence of common allergic response of delayed type to brucellosis antigen on the processes of glycolysis and dehydrogenase activity of krebs' cycle in guinea pigs' blood and organs was studied. along with inhibitory activity of four dehydrogenases investigated there was a depression of the glucolysis processes connected with reduction of the lactate, pyruvate content, and with depression of the ldh activity. an increase of the anaerobic fractions and lowering of aerobic fractions content ... | 1978 | 100150 |
| the effects of passive antibodies to egg albumin on active immunity in lambs to brucella abortus and egg albumin. | the long-term effects of colostrum on active immunity to two unrelated antigens are described. lambs were fed with pooled colostrum--to equalise passive immunity--with or without added antibodies to egg albumin (ea). there were significant breed differences in the response both to brucella abortus measured at one month of age, and to ea, measured at three months of age, although there was no significant correlation between the responses to the two antigens, either within or between breeds. surpr ... | 1978 | 100838 |
| [diagnostic value of agglutination reaction with buffered brucella antigen stained with rose bengal]. | a specific buffered antigen has been obtained, employing a method developed by the authors, stained with bengal rose and intended for performing a fast agglutination reaction to confirm brucellosis. the antigen produces a clear and demonstrative agglutination reaction with positive sera. practically, the test is readily carried out, and can be made a routine both in every serologic laboratory and for investigations under field conditions. the reaction produced with this antigen can determine dep ... | 1978 | 100935 |
| surface immunoglobulin in immunoproliferative diseases. | malignant b cells may originate from any of the stages of differentiation of b cells, primarily from igm bearing b cells. the malignant b cells maintain a limited potential for differentiation. in addition to surface immunoglobulins, various markers may be present on the b cell surface; when surface ig cannot be identified, these markers are used to identify b cells. however, for practical purposes, the detection of surface ig is most important in the identification of b cells in immunoprolifera ... | 1978 | 101125 |
| immunity to brucella in mice vaccinated with a fraction (f8) or a killed vaccine (h38) with or without adjuvant. level and duration of immunity in relation to dose of vaccine, recall injection and age of mice. | immunity to brucella in the mouse, assessed by bacterial spleen counts 15 days after intraperitoneal inoculation of a standard challenge of b. abortus 544, has been studied with two vaccines, one experimental, composed of a fraction of the bacterial cell-wall (f8) extracted from b. abortus 99, the other of killed whole bacteria, b. melitensis 53 h38, taken as reference (h38). the level of primary immunity depended on the dose of vaccine, the presence of oil adjuvant and the age of the mouse. the ... | 1978 | 101226 |
| [redox processes during sensitization and allergic reaction to microbial antigens]. | the effect of sensitization and delayed allergic response (dar) to brucellosis antigen on the processes of glycolysis and dehydrogenase activity of krebs' cycle was studied. the increasing sensitivity to brucella was accompanied by inhibition of the dehydrogenase activity and increasing glycolysis. the changes were more pronounced in sensitization to living brucella. in dar not only the dehydrogenase activity but also the processes of glycolysis were inhibited. reduction of the aerobic fractions ... | 1978 | 101256 |
| murine macrophage-lymphocyte interactions: scanning electron microscopic study. | light and scanning electron microscopic observations revealed murine macrophage-lymphocyte interactions involving the initial contact of peritoneal, spleen, or thymus lymphocytes with peritoneal macrophage processes or microprocesses followed by clustering of lymphocytes over the central nuclear area of the macrophages. lymphocyte-lymphocyte clustering was not observed in the absence of macrophages. attachment and subsequent clustering appeared not to require the presence of serum or antigen; th ... | 1978 | 101458 |
| plaque-forming cells in mice after experimental infection with brucella abortus. | cells producing antibody to brucella lipopolysaccharide were detected in spleens of mice infected with brucella abortus 19 by a hemolytic plaque assay. the appearance of immunoglobulin m-producing cells preceded humoral antibodies. the primary plaques were observed 5 days after inoculation, and they were still present by day 70. | 1978 | 101461 |
| antibody response to antigens distinct from smooth lipopolysaccharide complex in brucella infection. | the smooth lipopolysaccharide complex of the outer surface of smooth brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. sera from cattle vaccinated or infected with b. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. the brucella organism contains a large number of other antigenically distinct components. the biological significance of some of these antigens has b ... | 1978 | 101470 |
| congenital brucellosis in cattle associated with localisation in a hygroma. | a heifer born to a cow experimentally infected with brucella abortus strain 544 and reared in isolation from its birth was found infected. antibodies appeared when it was 10 months old. the heifer calved normally. brucella abortus of the same characteristics as strain 544 was recovered from a hygroma and from lymph nodes and udder. five heifers out of 55 (about 10 per cent) born to infected dams and reared in isolation from their birth were found to be infected. | 1978 | 102074 |
| age-related humoral antibody responses of akr/j mice to t-cell dependent and independent antigens. | | 1978 | 102588 |
| comparison of sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of brucella abortus infection in cattle. | a study was conducted to compare the sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of brucella abortus infection in cattle. cattle used were infected with b. abortus field strains or strain 19. peripheral blood was collected, and lymphocytes for the technique. the blood for the whole-blood lymphocyte stimulation assay was diluted 10-fold with rpmi 1640 medium (without additional serum supplement) and cultured. the two tests ... | 1978 | 102656 |
| resistance to tumor graft in mice treated with inactivated brucella abortus cultured in smooth or rough phase. | because killed brucella abortus organisms cultured in smooth (s) or rough (r) phase were known to differentially influence humoral and cellular immune responses and to differ in their effects on t-dependent responses, the antitumor properties of killed b. abortus organisms, cultured in s- or r-phase and then inactivated, were compared in (c57bl/6 x dba/2)f1 female mice with the use of 6 different transplantable tumors. in solid tumors, the antitumor effects produced by s-preparations were never ... | 1979 | 102858 |
| a study of the spleen and blood of rats infected with massive doses of brucella abortus. | | 1978 | 102878 |
| destructive aortic valve endocarditis from brucella abortus: survival with emergency aortic valve replacement. | brucella abortus infection of the aortic valve caused acute aortic regurgitation leading to severe left ventricular failure in a 62-year-old man. he made an excellent recovery after emergency aortic valve replacement. this is the third reported case of successful heart valve replacement for brucella endocarditis and the second such case involving the aortic valve. | 1978 | 103238 |
| cell-mediated immune response after brucella abortus s19 vaccination. | cell-mediated immunity to brucella abortus s19 vaccine was measured in young heifers by the microassay for stimulation of protein synthesis (sps) with [3h]leucine and the skin test for delayed hypersensitivity. brucella melitensis protein allergen and a crude b abortus s19-soluble antigen were compared in the sps test. the sps test was negative in 5 unvaccinated heifers and strongly positive in 3 twice-vaccinated steers. however, the sps test was positive only in 13 of 30 s19-vaccinated heifers ... | 1978 | 103470 |
| macrophage activation and antitumor activity of a brucella abortus ether extract, bru-pel. | bru-pel and brucella abortus lipopolysaccharide (lps) were tested for both macrophage activation and antitumor activity in an artificial metastasis model. resting macrophages were rendered nonspecifically tumoricidal for mbl-2 lymphoblastic leukemia target cells by exposure to bru-pel at greater than or equal to 1 ng/ml of culture medium. b. abortus lps failed to activate macrophages in vitro at all concentrations tested. ip treatment of homozygous nude mice with bru-pel induced cytotoxic macrop ... | 1978 | 103619 |
| [survival capacity of certain pathogens under environmental conditions]. | | 1979 | 103703 |
| brucella antigen preparations for in vitro lymphocyte immunostimulation assays in bovine brucellosis. | three brucella antigen preparations, brucella abortus soluble antigen, b. abortus strain 45/20 enriched protein antigen, and b. melitensis enriched protein antigen, were compared in terms of their ability to induce specific in vitro lymphocyte immunostimulation responses. lymphocytes were prepared from peripheral blood of cattle with different exposure experiences to b. abortus organisms. lymphocytes were processed by the ficoll-diatrizoate technique, and results were assayed for [3h]tymidine in ... | 1978 | 103831 |
| soluble antigens of virulent and attenuated biotypes of brucella abortus. | several methods were used to characterize three brucella abortus biotypes (1, 5, and 7), including the attenuated vaccine strain s-19. chemical analysis did not reveal remarkable differences among these strains, and only minor differences were noted in elution patterns of soluble extracts subjected to column chromatography. qualitative and quantitative differences in extract components were demonstrated, however, by polyacrylamide gel isoelectric focusing. a distinctive difference was the presen ... | 1978 | 103842 |
| utilization of a specific in vitro lymphocyte immunostimulation assay as an aid in detection of brucella-infected cattle not detected by serological tests. | studies using the in vitro lymphocyte stimulation test (lst) were conducted with cattle in a dairy herd with a high percentage of reactors to several serological tests for brucellosis. lymphocytes were prepared from peripheral bovine blood by the ficoll-diatrizoate technique. lymphocytes were cultured using microtitration culture plates. brucella abortus soluble antigen, at a concentration of 4.4 microgram/culture, was added to the appropriate wells of microtitration culture plates and incubated ... | 1978 | 103888 |
| failure of routine methods in the diagnosis of chronic brucellosis. | a young man who was raised on a farm and developed recurrent subcutaneous abscesses is described. multiple cultures revealed either no growth or normal skin flora. routine serologic evaluation for brucella agglutinins was negative, including serial dilution to greater than 1:1,000 of the patient's sera. brucella abortus infection was documented by the use of a binding antibody assay to measure the patient's class-specific serologic response and obtaining a positive culture by incorporating methi ... | 1979 | 104395 |
| cell-mediated and humoral immune responses of cattle to brucella abortus, mycobacterium bovis, and tetanus toxoid: evaluation of immunization and assay techniques. | a concentration of 2.5 x 10(-5) m 2-mercaptoethanol (2-me) added to the medium in lymphocyte blastogenesis assays increased both the uptake of [3h]thymidine in unstimulated lymphocyte cultures and the probability of detecting antigen-sensitized cattle. the use of 2-me did not cause lymphocytes from unsensitized cattle to react positively in blastogenesis assays. a crude brucella lysate prepared from brucella abortus strain 19 was compared with a well-characterized brucella protein allergen prepa ... | 1978 | 104645 |
| cell-mediated and humoral immune responses of cattle to brucella abortus, mycobacterium bovis, and tetanus toxoid: immunization of the fetus. | | 1978 | 104646 |
| response of high and low antibody producer to brucella. | biozzi's high and low lines of antibody producers have been applied to an analysis of murine in vivo responses to brucella. high responders (h/ab) are better producers of anti-brucella antibodies and of brucella induced interferon than low responders (l/ab). there is no interstrain difference between h/ab and l/ab mice as regards to cutaneous hypersensitivity reactions to melitin. non-immunized l/ab mice are more resistant to infection with live brucella than h/ab. immunization with formalin kil ... | 1978 | 104653 |
| a serologic survey of mule deer and elk in utah. | sera from mule deer (odocoileus hemionus) and elk (cervus canadensis) in central and northern utah were tested for the prevalence of antibodies to 11 diseases communicable to man or domestic livestock. antibodies to francisella tularensis (at 1:20) were found in 47 of 88 (53.4%) elk and 1 of 89 (1.1%) deer. a screening slide agglutination test for titers to brucella (at 1:20) showed two reactors in elk but none in deer sera. no positive antibody titers were obtained in tests for anaplasmosis, co ... | 1978 | 105153 |
| [lysozyme concentration in the loose connective tissue of animals with different species sensitivity to brucellosis]. | lysozyme content was determined in the subcutaneous cellular tissue of guinea pigs and albino rats differing by species resistance to brucella infection. experiments were conducted on intact animals and those inoculated subcutaneously with live br. abortus 19-ba vaccine. connective tissue was taken from the site of the vaccine administration and from the contralateral side (control). observations showed connective tissue of the animals highly sensitive to brucella infection to be exceedingly poo ... | 1978 | 105522 |
| brucella cultures typed by the who brucellosis centre at the commonwealth serum laboratories, melbourne. | results of the typing of brucella cultures received at the who brucellosis centre, csl, melbourne, from 1968-1976 are presented. the distribution of the biotypes of cultures recovered throughout australia is shown on a host and state basis and atypical cultures are discussed. cultures identified from australia were br. abortus, biotypes 1, 2 and 4 and strain 19; br. suis, biotype 1 and br. ovis. br. melitensis biotypes 1, 2 and 3 were recorded only as exotic human infections from the mediterrane ... | 1978 | 105700 |
| enhancement of resistance to murine osteogenic sarcoma in vivo by an extract of brucella abortus (bru-pel): association with activation of reticuloendothelial system macrophages. | the administration of an aqueous-ether extracted residue of brucella abortus (bru-pel) inhibits development of transplanted osteogenic sarcomas in mice as evidenced by a decrease in mortality. at least one mechanism through which bru-pel modulates host resistance is activation of macrophages of the reticuloendothelial system. peritoneal macrophages harvested from mice receiving bru-pel were cytotoxic for osteogenic sarcoma cells in vitro, limited the replication of vaccinia virus in cell culture ... | 1979 | 106004 |
| macrophage activation during experimental murine brucellosis: a basis for chronic infection. | evidence is presented that the chronicity of infection in cba mice after injection of brucella abortus 19 is related to a number of factors: (i) the relative resistance of b. abortus to macrophage killing, which allowed some bacteria to survive the peak of macrophage activity occurring at 14 days; (ii) the decline in macrophage activity thereafter (this decline was related in part to the presence of fewer bacteria to stimulate the bactericidal activity and also to specific, active suppressor mec ... | 1979 | 106010 |
| immunofluorescent studies of the local immune response in the mammary glands of rats. | two irritants, phosphate-buffered saline and alcohol, and antigens including killed brucella abortus, live b. abortus, staphylococcus aureus, and rat parvovirus were separately infused into rat mammary glands during pregnancy, and by using immunofluorescent techniques, the numbers of immunoglobulin-containing cells in glands during lactation and involution were determined. the study provided basic information on the local immune response of the mammary gland to antigens of various types. in all ... | 1979 | 106012 |
| [accelerated diagnostic cultivation of pathogenic environmental microbes. ii. accelerated diagnostic cultivation of the causative agents of anthropozoonotic plague, glamders, melioidosis, brucellosis and tularemia]. | | 1978 | 106632 |
| immunochemical studies on a yersinia enterocolitica o:9 lipopolysaccharide cross-reacting with brucella abortus and vibrio cholerae extracts. | the presence of two distinct lipopolysaccharides in yersinia enterocolitica o:9 is described: one isolated from the aqueous phase and one from the phenol phase (westphal system). the sugar moiety of the phenol phase lipopolysaccharide has been identified as being responsible for the serologic cross-reaction of y. enterocolitica o:9, brucella abortus and vibrio cholerae. the phenol phase antigen consists of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid, heptose, lipid a and a protei ... | 1978 | 106756 |
| a study of the macro and micro immunoglobulins to brucella abortus and their transfer from cow to calf via the colostrum. | samples of adult bovine serum, colostrum, the serum of unsuckled and suckled calves were subjected to gel filtration through sephadex g 200. all samples (except those of unsuckled calves) contained antibodies to brucella abortus. four phases were recorded in normal adult serum corresponding to igm, igg, albumin and a fourth phase of smaller nitrogen-containing molecules. in colostrum phase 2 was relatively large and phase 3 was absent indicating absence of albumin. in the serum of the unsuckled ... | 1978 | 106762 |
| the relationship of brucella abortus titers to equine fistulous withers in ethiopia. | | 1979 | 107647 |
| immunity and health in young lambs. | | 1978 | 107648 |
| resistance of normal or immunized guinea pigs against a subcutaneous challenge of brucella abortus. | guinea pigs were inoculated subcutaneously into a hind footpad with brucella abortus. unimmunized guinea pigs had little capacity for barring brucella from spreading to their spleens: the inoculum dose needed to infect 50% of the spleens one month after inoculation was about 10 brucella. when the size of the challenge inoculum reached approximately 5 x 10(3) id50 a previous immunization with killed or virulent brucella: --did not prevent the challenge bacteria from reaching the lymph nodes near ... | 1978 | 107845 |
| a solid-phase radioimmunoassay for the determination of bacterial-specific antibodies within different immunoglobulin classes: application to bovine brucella abortus antibodies. | a solid-phase radioimmunoassay (ria) has been developed for quantitation of class-specific antibodies against bacteria. brucella abortus cells were used to sensitize glass tubes after sedimentation, drying and methanol fixation. bovine antibodies that attached to the bacteria were detected by binding of a rabbit antiserum specific for each class or subclass of bovine immunoglobulins followed by binding of 125i sheep anti-rabbit immunoglobulin reagent. this three-step method was adapted as it was ... | 1978 | 107846 |