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investigations of the structure of 3-methylcrotonyl-coa carboxylase from achromobacter.it was shown by gel electrophoresis in sodium dodecylsulphate solution that 3-methylcrotonyl-coa carboxylase from achromobacter ivs is composed of two different subunits with molecular weights of about 78000 and 96000, respectively. the biotin is bound to the heavier subunit. it was previously found that 3-methylcrotonyl-coa carboxylase contains four biotin molecules per complex. a complex composed of four of each subunit would thus have a molecular weight of about 700000. this is compatible wit ...19751267
determination of d-3-hydroxybutyrate dehydrogenase in mouse pancreatic islets with a photokinetic technique using bacterial luciferase.a sensitive assay for d-3hydroxybutrate dehydrogenase (ec 1.1.1.30) was developed for use with the minute amounts of material obtained from islets of langerhans microdissected from freeze-dried pancreatic sections. nadh formed in the enzyme reaction was determined by photokinetic analysis of the luminescence obtained with bacterial luciferase from achromobacter fishcherii. in this way, accurate determination was obtained with less than 0.1 mug dry weight of islet material. in obese hyperglycemic ...19764298
purification, stability and inhibition of the collagenase from achromobacter iophagus. 19756314
biosynthesis of chitinase by achromobacter liquefaciens.bacterial cultures under study synthesize exocellular chitinase on a medium containing chitin or demineralized crab shells as a source of carbon and nitrogen. conditions for biosynthesis of chitinase by the cells of achromobacter liquefaciens 301a were investigated under periodic and continuous conditions of cultivation. the preparation of chitinase isolated from the cultural broth of a. liquefaciens 301a hydrolysed colloid and native chitin at the optimum ph 6.5 and temperature 40degreesc. the ...197612452
bacteriolysis by immobilized enzymes.bacteriolytic enzymes produced by achromobacter lunatus were immobilized in collagen membrane. intact bacteria such as pseudomonas solanacearum, xanthomonas oryzae, staphylococcus aureus, and pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. relative activity of the bacteriolytic enzyme-collagen membrane against pseu. solanacearum was about 2% of that of native bacteriolytic enzymes. no difference in the optimum ph was observed between immobilized enzymes and nat ...197714747
continuous production of nadp by immobilized achromobacter aceris cells.several microorganisms having higher nicotinamide adenine dinucleotide kinase (nad kinase, ec 2.7.1.23) activity were immobilized into polyacrylamide gel lattices. the enzyme activity field by immobilization was highest in achromobacter aceris aku 0120. by the incubation of the immobilized a. aceris cells at ph 4.0, the nad kinase activity increased and the adenosine triphosphate (atp)-degradation activity disappeared completely. enzymatic properties of the immobilized a. aceris cells were inves ...197824483
new experiments of biotin enzymes.the objects of structural studies on biotin-enzymes were acetyl coa-carboxylase and pyruvate carboxylase of saccharomyces cerevisiae and beta-methylcrotonyl coa-carboxylase and acetyl coa-carboxylase of achromobacter iv s. it was found that these enzymes can be arranged in three groups. in the first group, as represented by acetyl coa-carboxylase of achromobacter, the active enzyme could be resolved in three types of functional components: (1) the biotin-carboxyl carrier protein, (2) the biotin ...197941682
studies on the biodegradation of nonionic surfactants applied in the polyester fiber industry. i. activated sludge bacteria degrading the surfactants.the paper presents characteristics of 76 strains of bacteria capable of utilizing nonionic surfactants cirrasol fp, cirrasol sf 200 and cirrasol tcs as the source of carbon. the strains were isolated from two activated sludges adapted to the purification of wastes containing the above compounds at concentration 150--200 mg/l. the isolated strains belonged to the genera: achromobacter, alcaligenes, arthrobacter, flavobacterium, mycobacterium, nocardia, pseudomonas and xanthomonas. with load 0.11 ...197662497
the effect of cyclohexane derivatives on selection of bacterial groups forming activated sludge microflora.the effect of cyclohexanol, cyclohekxanon and cyclohexylamine on the selection of bacteria in a model population composed of bacteria isolated from activated sludge was examined. the initial population consisted of both gram-positive and gram-negative bacteria. the latter, which accounted for 90-97% of the population, belonged mainly to three pseudomonas groups and the enterobacteriaceae, vibrio-aeromonas, achromobacter-alcaligenes and flavobacterium groups. seven day growth in medium containing ...197880928
bacterial utilization of cyclo(glycl-l-prolyl).3 strains of soil bacteria (2 achromobacter, 1 flavobacterium) were isolated by growth on cyclo(gly-l-pro) as carbon/nitrogen source. good growth required yeast extract supplements. utilization of cyclo(gly-l-pro) was inducible. many efforts failed to obtain active cell-free preparations. injected radioactive cyclo(gly-l-pro) was excreted intact by the albino rat; in contrast, injected radioactive h-gly-l-pro-oh was extensively metabolized.197895965
[products of bacterial destruction of sodium dodecyl sulphate].the products of na dodecyl sulphate destruction by the three bacterial cultures--pseudomonas aeruginosa, flavobacterium devorans, achromobacter guttatus--were examined. the cultures were shown to decompose na dodecyl sulphate in a similar way. the primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. products of bacterial destruction of alkyl sulphates did not show foam forming c ...1979117448
over-production of porphyrins and heme in heterotrophic bacteria.in escherichia coli, pseudomonas aeruginosa, and achromobacter metalcaligenes gamma-aminolevulinic acid synthase and gamma-aminolevulinic acid dehydratase appear to be the rate limiting enzymes of porphyrin and heme biosynthesis. bypassing of these enzymes by addition of gamma-aminolevulinic acid or porphobilinogen results in overproduction of tetrapyrroles.1975126586
clostridium welchii corneal ulcer--a case report.a case of corneal ulcer in a 10-year-old nigerian boy in which clostridium welchii and achromobacter were isolated is reported. the absence of trauma and rarity of only corneal involvement in a clostridial infection is emphasized. the bacteriology of the clostridial infection is emphasized. the bacteriology of the clostridial ocular infections is reviewed, the media necessary for isolation of the organism and the symbiotic relationship with other bacteria are discussed. the clinical course of th ...1975164996
collagenase production by achromobacter iophagus.achromobacter iophagus produced collagenase (ec 3.4.24.3) when cultured aerobically in buffer containing 5% peptone. the bacterium is non-pathogenic and tests on rabbits indicated that the culture medium was atoxic. the collagenase, which hydrolyzed insoluble and soluble native collagen, was purified by (nh4)2 so4 precipitation, starch block electrophoresis, and gel filtration. it was shown to be serologically distinct from clostridium histolyticum collagenase and to have molecular weight and se ...1975165833
specificity of collagenase from achromobacter iophagus. 1975169160
inhibition of collagenase from achromobacter iophagus by synthetic peptides. 1975173543
chemical characterization and study of the autodigestion of pure collagenase from achromobacter iophagus.only one collagenase (ec 3.4.24.3) is produced by the non-pathogenic achromobacter iophagus strain. the chromatography of the crude enzyme on de-32 cellulose followed by gel filtration on sephadex g-100 in the presence of 1 m sodium chloride led to the isolation of a homogeneous enzyme. its specific activity (1.642 mukat/mg) represents the highest value ever obtained for a bacterial collagenase. the amino acid composition of a. iophagus collagenase differs from that of clostridium histolyticum m ...1976177067
achromobacter cycloclastes nitrite reductase. the function of copper, amino acid composition, and esr spectra.1. dialysis against cyanide at ph 7 of achromobacter cycloclastes nitrite reductase [ec 1.7.99.3] of a dissimilatory type led to the removal of about 50% of the copper from the enzyme molecule, with a concomitant decrease of the enzymatic activities. it was inferred that enzyme-bound copper atoms play an essential role in the catalytic activities of the enzyme. 2. the amino acid composition of the enzyme was determined after acid hydrolysis. 3. esr spectra of the frozen solution and lyophilized ...1975179983
cleavage of beta-casein by collagenases from achromobacter iophagus and clostridium histolyticum. 1976182537
the induction of collagenase and a neutral proteinase by their high molecular weight substrates in achromobacter iophagus. 1976187752
microassay with the nadh-induced light reaction, technique improved by means of purified enzymes from achromobacter fischeri.purification of a commercial preparation of achromobacter fischeri was carried out by agarose-suspension electrophoresis and by molecular-sieve chromatography. both the luciferase and an oxidoreductase, catalyzing reduction of fmn with nadh, were obtained in more than one form. flavins, liable to interfere with the light production in analytical applications, were present in amounts worthy of consideration, but seem to be firmly bound to protein. the major quantity was found in the enzymatically ...1977198648
synthesis of peptide inhibitors of the collagenase from achromobacter iophagus. 1977201387
subunit structure of achromobacter collagenase.the highly active form of collagenase (ec 3.4.24.3) from achromobacter iophagus (specific activity 2 microkat/mg) has a molecular weight of 70,000 and the sedimentation coefficient s20,2 = 4.4 s. it is composed of two subunits of molecular weight 35,000 and s20,w of 2.9 s. the dissociation of the dimer under different conditions resulted in the complete and irreversible loss of enzymic activity. a unique n-terminal sequence thr-ala-ala-asp-leu-glu-ala-leu-val- indicates that the two subunits are ...1978202322
specificity of the collagenolytic enzyme from the fungus entomophthora coronata: comparison with the bacterial collagenase from achromobacter iophagus. 1979219780
some newly characterized collagenases from procaryotes and lower eucaryotes.chemical and enzymatic properties of four collagenases newly isolated from anaerobic clostridium histolyticum, aerobic achromobacter iophagus, and from two lower eucaryotes, the fungus entomophthora coronata and the insect hypoderma lineatum are reviewed. the problems of their biosynthesis and precursors, namely the effect of induction of collagenase and neutral proteinase in achromobacter by their macromolecular substrates are discussed. the two bacterial collagenases are zn-metallo-enzymes; th ...1979220520
differences in the degradation of native collagen by two microbial collagenases.the early stages of degradation of native collagen by two bacterial collagenases were studied by electron microscopy and by automatic edman degradation. the purified collagenase from clostridium histolyticum was shown to cleave native collagen at several sites, but not progressively from the n-terminus, as had been previously suggested. the homogeneous collagenase from achromobacter iophagus cleaves native collagen preferentially at two sites corresponding to the interbands 33-34 and 41-42. the ...1979224860
crystalline l-histidine ammonia-lyase of achromobacter liquidum. crystallization and enzymic properties.crystalline l-histidine ammonia-lyase of achromobacter liquidum was prepared with a 24% recovery of the activity. the specific activity of the pure enzyme (63 mumol of urocanic acid min-1 mg-1) is similar to those so far reported for the enzyme from other sources. the purified enzyme appeared to be homogeneous by analytical disc electrophoresis and isoelectric focusing (pi = 4.95). the molecular weight determined by sephadex g-200 gel filtration is 200000. the optimum ph is 8.2, and the optimum ...1975240693
achromobacter l-glutaminase-l-asparaginase: human pharmacology, toxicology, and activity in acute leukemias. 1979288507
[in vitro activity of cefoxitin compared with the activity of other antibiotics and trimethoprim-sulfamethoxazole (author's transl)].the new developed antibiotic cefoxitin has been tested in vitro on its effectiveness against bacteria isolated from human material. pseudomonas aeruginosa and streptococci of the serological group d were not tested, because these microorganisms are not sensitive against this antibiotic. besides cefoxitin other antibiotics (cephalothin, cephalexin, ampicillin, carbenicillin, tetracycline, gentamicin, penicillin g, oxacillin) and trimethoprim-sulfamethoxazole were also tested. as to the results ce ...1978347234
community-acquired bacteremic achromobacter xylosoxidans type iiia pneumonia in a patient with idiopathic igm deficiency.a man was hospitalized with bacteremic achromobacter xylosoxidans type iiia pneumonia. the authors are aware of no previously reported similar infections caused by this bacterium. a clinical cure was achieved with a combination of carbenicillin and kanamycin therapy. microtiter susceptibility testing revealed that carbenicillin was the antibiotic to which a. xylosoxidans iiia was most sensitive (minimal inhibitory concentration, 1.6 microgram/ml) and that synergy between carbenicillin and kanamy ...1978360830
achromobacter species (cdc group vd): morphological and biochemical characterization.twenty-three isolates of achromobacter species (cdc group vd) were examined morphologically and biochemically. gram stains revealed gram-variable bacilli frequently curved or hooked at one pole and often coryneform in shape and arrangement. electron microscopy revealed the presence of extracellular material in polar accumulations and demonstrated the polar flagella arrangement seen by light microscopy to be lateral. two colony types were produced; one was minute and watery at 24 h (35 degrees c) ...1979379035
semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria.a simple and rapid semiquantitative slide catalase test useful for the identification of oxidative and nonsaccharolytic gram-negative bacteria, i.e., "nonfermenters," is described. using the interpretative criterion of time of appearance of oxygen bubbles in 3% hydrogen peroxide, three categories of nonfermenters were established. the rapid catalase producers included achromobacter xylosoxidans and achromobacter species; acinetobacter anitratus and acinetobacter lwoffii; bordetella bronchiseptic ...1979393719
treatment of l5178y tumor-bearing bdf1 mice with a nonimmunogenic l-glutaminase-l-asparaginase.an l-glutaminase-l-asparaginase from achromobacter has been rendered nonimmunogenic by the covalent attachment of polyethylene glycol (peg) to nonessential amine groups of the enzyme. peg-l-glutaminase-l-asparaginase exhibits a greatly enhanced half-life in the bloodstream compared to the unmodified enzyme in normal mice, and is effective in prolonging the survival of bdf1 mice inoculated ip with l5178y cells. peg-l-glutaminase-l-asparaginase appears rapidly in the blood following ip injection.1979466651
[effect of formate on the respiration of different microorganisms].the cells of methylotrophic bacteria (achromobacter parvulus 1, pseudomonas methylica 2 and 20, ps. fluorescens 45, ps. oleovorans 52) and candida methylica 101 grown in a medium with methanol take up oxygen in the presence of formiate. a. parvulus is most resistant to formiate (km = 2.3 +/- 0.5) x 10(-3) m; ki = 4 x 10(-1) m). when grown on cn substrates, these microorganisms cannot oxidize formiate (with an exception of a. parvulus). formiate also serves as a respiration substrate for e. coli ...1979481274
studies with mechanism-based inactivators of lysine epsilon-transaminase from achromobacter liquidum.analogues of lysine containing a 4,5-acetylenic linkage (lysyne) or a cis- or trans-4,5-olefinic linkage (lysenes) function as substrates for a homogeneous l-lysine epsilon-transaminase from achromobacter liquidum but partition between transamination and time-dependent inactivation. the partition ratio is lowest for lysyne (40 per inactivation event) and higher for trans-lysene (160 per inactivation event), and the cis-lysene transaminates 1600 times per inactivation event. cis-lysene yields alp ...1979486402
stimulation of l-asparate beta-decarboxylase formation by l-glutamate in pseudomonas dacunhae and improved production of l-alanine.the formation of l-asparate beta-decarboxylase by pseudomonas dacunhae was compared on media containing a variety of organic acids and amino acids as a carbon source. although the enzyme was formed constitutively when the organism was grown on basal medium or on that containing tricarboxylic acid cycle intermediates, it was induced twofold by l-glutamate and repressed one-tenth by l-serine. l-glutamine, l-proline, l-leucine, glycine, and l-threonine also showed induction effects lower than that ...1979533269
[an achromobacter xylosoxidans strain isolated from pleural empyema (author's transl)].the isolation of an achromobacter xylosoxidans strain from a 32 year old man with pleural empyema, and its biochemical characteristics are reported.1979554008
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
[pathways of assimilation of carbon oxides in carboxydobacteria seliberia carboxydohydrogena and achromobacter carboxydus].assimilation products of 14c-bicarbonate and carbon-14c oxide were studied in two carboxydobacteria seliberia carboxydohydrogena and achromobacter carboxydus which differed in their ability for chemolithoautrophous growth in the presence of hydrogen. the dynamics and composition of labeled products formed upon assimilation of 14c-bicarbonate in the presence of unlabeled carbon oxide by the two organisms, the composition of products formed upon assimilation of 14co by suspensions of s. carboxydoh ...1977600091
observation on the bacterial population of the os cervix of the ewe before and after embryo death.bacterial samples were isolated from the os cervix of mature merino ewes during a normal oestrous cycle and after colchicine treatment which killed the 25-day old embryos. the population of bacteria was small during the oestrous cycle and consisted of achromobacter spp. alcaligenes spp, corynebacterium spp, bacillus spp and escherichia coli. there was a significant increase in the numbers of bacteria isolated and a change in the proportions of the bacteria isolated from ewes after embryo death. ...1977612334
properties of taurine: alpha-ketoglutarate aminotransferase of achromobacter superficialis. inactivation and reactivation of enzyme.the activity of taurine: alpha-ketoglutarate aminotransferase (taurine: 2-oxoglutarate aminotransferase, ec 2.6.1.55) from achromobacter superficialis is significantly diminished by treatment of the enzyme with (nh4)2so4 in the course of purification, and recovered by incubation with pyridoxal phosphate at high temperatures such as 60 degrees c. the inactive form of enzyme absorbing at 280 and 345 nm contains 3 mol of pyridoxal phosphate per mol. the activated enzyme contains additional 1 mol of ...1978629994
achromobacter xylosoxidans isolates in hawaii.clinical and bacteriological features of nine cases in which achromobacter xylosoxidans were isolated in hawaii are described. five cases were ear infections mixed with other gram-negative bacteria. colonial morphology, xylose oxidation, peritrichous flagella staining, and antibiotic susceptibility pattern assisted in separating this bacterium from other nonfermentative, oxidase-positive, gram-negative rods.1978632351
cerebral ventriculitis associated with achromobacter xylosoxidans.six patients in the neurosurgical ward of fukushima medical college hospital suffering from ventriculitis due to achromobacter xylosoxidans infection had undergone craniotomy or cranial trepanation before the infection. the strains of a. xylosoxidans isolated from the patients were resistant to streptomycin, ampicillin, cephaloridine, gentamicin, and colistin. they were also resistant to chlorhexidine digluconate (hibitane) in a concentration of 2%. when a study of the chlorhexidine used in the ...1978632360
taxonomy of alcaligenes and achromobacter by polyacrylamide gel electrophoresis of their soluble proteins [proceedings]. 1978655695
[clinical and bacteriological studies on infections due to achromobacter xylosoxidans (author's transl)]. 1978660928
identification of achromobacter species by cellular fatty acids and by production of keto acids.the cellular fatty acid composition and metabolic products of 12 reference strains of achromobacter sp. and a. xylosoxidans were determined by gas-liquid chromatography (glc). results showed that the two achromobacter groups are strikingly different and can be readily distinguished on the basis of cellular fatty acids and the short-chain acids produced by achromobacter sp. the major cellular fatty acids of achromobacter sp. were octadecenoic (18:1) and a 19-carbon cyclopropanoic (19:0 delta) aci ...1978670387
phage growth characteristics on stationary phase achromobacter cells.the growth characteristics of alpha3a bacteriophage on stationary phase achromobacter strain 14 are described. phage alpha3a growth on stationary phase cells is characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 710 p.f.u./cell as compared with 153 p.f.u./cell in exponential wild type cells. during the latent period the infected cells are very sensitive to changes in growth conditions and in particular, dilution. pre-conditioning of the bacterial cells ...1978722281
bacterial glutaminase in treatment of acute leukaemia.a glutaminase-asparaginase enzyme from achromobacter sp has antitumour activity in vitro and in animals. glutaminase was administered in doses of 3500-20 000 iu/m2 body surface area/day to six patients with acute lymphoblastic leukaemia (all) and three patients with acute myeloid leukaemia (aml). the enzyme had a blood half life of 80 minutes but depletion of blood glutamine persisted for 12 hours after single doses. seven patients, including four (two with aml and two with all) resistant to asp ...1976773514
bacterial degradation of polychlorinated biphenyls ii. rate studies.polychlorinated biphenyls (aroclor 1221, 1242 and 1254) at concentrations up to 0.1% in glucose did not inhibit the growth of lake water bacteria. the bacteria used aroclor 1221 and 1242 but not 1254 as sole carbon and energy sources for growth. less than 1% of lake water bacteria, however, possess this ability. seven bacterial isolates from aroclor agar plates were identified; five belonged to achromobacter sp. and two were pseudomonas sp. the metabolic breaksown of aroclor 1221 was followed. t ...1975804943
[a hospital epidemic due to achromobacter calcoaceticus].35 patients were secondarily infected in our hospital with a strain of a. calcoaceticus resistant to the usual antibiotics and sulfonamides, but sensitive to colimycin. the epidemic lasted 118 days and is not yet finished. each of the infected patients had a severe surgical or medical illness, underwent operations, trachetomy, etc. and was treated with antibiotics. a. calcoaceticus persisted alone or was associated with other bacteria from 1 to 46 days, in specimens (sputum, etc. or in blood) so ...1975807899
[microflora of active ooze participating in the decomposition of sulfanilic acid].microflora of domestic water can be a source of active ooze adapted to sulphanilic acid. adaptation of the microflora to sulphanilic acid at a concentration of 170-200 mg/l takes 6 to 8 days. the microflora of active ooze, immediately after adaptation, consists mainly of pseudomonas species, ps. denitrificans, ps. fluorescens, ps. striata, ps. putida, etc., and also of achromobacter stutzeri, achromobacter flavum, mycobacterium phlei, mycobacterium mucosum, bacillus mesentericus, bac. cereus, sa ...1975808686
[hydrogen and carboxide bacteria belonging to the microflora of degradation].the following bacteria oxidizing hydrogen and/or carbon monoxide were shown by cultivating in the concentration gradient of acetate, lactate, and glucose, to belong to the microflora of dispersal: hydrogenomonas eutropha z-1, h. pantotropha z-11, pseudomonas carboxydoflava z-1107, achromobacter carboxydus z-1171, paracoccus denitrificans, and alcaligenes paradoxus.1976820943
effects of thermoradiation on bacteria.a 60co source was used to determine the effects of thermoradiation on achromobacter aquamarinus, staphylococcus aureus, and vegetative and spore cells of bacillus subtilis var. globigii. the rate of inactivation of these cultures, except vegetative-cell populations of b. subtilis, was exponential and in direct proportion to temperature. the d10 (dose that inactivates 90% of the microbial population) value for a. aquamarinus was 8.0 krad at 25 degrees c and 4.9 krad at 35 degrees c. for s. aureus ...1976823869
microbiological characteristics of natural mineral water.natural, non-carbonated mineral water is, like every other natural water from a spring, never sterile. however, the microbial level is always very low. but after its bottling, the level rises rapidly and numbers of more than 10,000 to 100,000/ml can be reached. in principle 2 groups of bacteria of very different origin and properties can be found in the microbial flora of the bottled, non-carbonated mineral water. allochthonous bacteria will get into the water by contamination from the container ...1976829205
rifampicin-resistant mutant supporting bacteriophage growth on stationary phase achromobacter cells.a rifampicin-resistant achromobacter mutant with an altered rna polymerase was isolated. the mutant supports phage alpha3a growth in both log and stationary phase cells. phage growth on stationary phase cells is sensitive to aeration and growth only occurs at oxygen concentrations of less than 5-2 p.p.m. the rifampicin-resistant mutant is similar to the spontaneous mutant strain 14 reported by woods (1976) in that both mutants support stationary-phase phage growth under micro-aerophilic conditio ...1977859010
[effect of humic acid on formation and several properties of the extracellular polysaccharide of achromobacter delicatulus]. 1977865307
[physiology of aniline catabolism by achromobacter ir2].a bacterium was isolated from soil which utilizes aniline as sole source of carbon and nitrogen. it was identified as achromobacter sp. the cells grow at concentrations in the range of 0.5 to 1.25 mg aniline/ml with a growth rate of 0.3 h-1. substrate inhibition was observed at concentrations higher than 1.5 mg/ml, 3.0 mg/ml completely inhibit the growth. the yield coefficient was 0.63. aniline was degraded with an activity of 200 microng/mg cell dry weight/hour. aniline was assimilated and comp ...1977868083
[attempt to establish genome similarity between alcaligenes faecalis and several gram-negative bacteria using the method of molecular hybridization of dna to dna].the technique of dna-dna molecular hybridization was used to demonstrate the absence of genome similarity between alcaligenes faecalis and gram-negative bacteria belonging to the genera pseudomonas, enterobacterium, comamonas producing alkalis, and vibrions. some similarity in the polynucleotide sequences was found between the dna of the reper strain of alcaligenes faecalis 45 (5--10% of homology) and the dna of the culture belonging to the achromobacter genus; therefore, the two genera may belo ...1977870806
[content of nitrogen bases in the dna of carboxide bacteria].the composition of nitrogen bases was determined in total dna of live strains of carboxide bacteria belonging to the genera pseudomonas, comamonas, seliberia (agrobacterium), achromobacter. considerate differences in the nucleotide composition of dna of the studied bacteria confirm that they indeed belong to different genera. at the same time, the content of gc pairs in dna of these bacteria is within the range found for the corresponding genera by other authors.1977882021
strains of achromobacter xylosoxidans from clinical material.eleven strains of achromobacter xylosoxidans have been received from among 1106 strains of gram-negative, non-fermentative bacteria submitted to the national collection of type cultures for computer-assisted identification since 1 january 1972. the strains showed resistance to a wide range of antimicrobial agents and five of the isolates possibly played a pathogenic role. the biochemical characteristics of these 11 strains were compared with those of three culture collection strains.1977886012
metabolism of quaternary carbon compounds: 2,2-dimethylheptane and tertbutylbenzene.two achromobacter strains capable of utilizing 2,2-dimethylheptane or tertbutylbenzene as the sole carbon and energy source were isolated from waste-water. pivalic acid was found in the cultures of achromobacter a1 containing 2,2-dimethylheptane. from cultures of achromobacter a2 in the presence of tertbutylbenzene, a diol was isolated and identified as 2,3-dihydro-2,3-dihydroxytertbutylbenzene. evidence for meta cleavage of the aromatic ring and for accumulation of pivalic acid in the cultures ...1977921261
6-aminohexanoic acid cyclic dimer hydrolase. a new cyclic amide hydrolase produced by achromobacter guttatus ki74. 1977923591
isolation of oxidase-positive gram-negative cocci not belonging to the genus neisseria from the urogenital tract.in a 12-month period, oxidase-positive, gram-negative cocci showing similar characteristics in biochemical tests have been isolated from the urogenital tract of 39 male and female patients. although these organisms superficially resemble neisseria gonorrhoeae, biochemical characterization and the results of dna base composition analysis indicate that they do not belong to the genus neisseria. the relationship of these organisms to the genera neisseria, achromobacter, and pseudomonas is discussed ...1976939808
[studies on the antibacterial activity of the combination sulfamoxole/trimethoprim (cn 3123) (author's transl)].after giving a survey on the situation of antibiotic resistance in the region of northern bavaria during 1973/74 and comparing the activity of a sulfamethoxazole (smz) trimethoprim (tmp) combination to other commonly used antibiotics and chemotherapeutic agents, the results of tests with the new combination of n1-(4,5-dimethyl-2-oxazolyl)-sulfanilamide) (sulfamoxole) and 2,4-diamino-5-(3,4,5-trimethoxy-benzyl)-pyrimidine (trimethoprim) at a ratio of 5:1 (cn 3123; nevin, supristol) are compared t ...1976947327
divalent cations in the envelope of a psychrophilic achromobacter.the function of ca2+ in a psychrophilic achromobacter, previously found to bind large amounts of these ions to its envelope, has been studied. bacteria suspended in media of low ionic content showed decreases in wet weight, dry weight and growth capacity, and increases in light scattering and in the release of u.v.-absorbing substances into the medium. the permeability barrier to ca2+ was also damaged, and there was a release of radioactivity from bacteria labelled with 45ca2+. these events occu ...1976950556
bacteriophage growth on stationary phase achromobacter cells.a new phage-host system is described in which phage alpha 3a grows on stationary phase achromobacter mutant strains. characteristic clear plaques are formed, at an e.o.p. 10(-1) to 10(-2), on already confluent bacterial lawns of the mutant strains. phage growth is sensitive to aeration and growth only occurs under micro-aerophilic conditions. lysates prepared on the mutant strains cannot transduce in contrast to transducing lysates prepared from wild type achromobacter strains.1976956787
acinetobacter and similar organisms in ear infections.fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. after comparison with eight reference strains, 32 of the isolates were identified as acinetobacter anitratus, 22 as acinetobacter iwoffii, three as moraxella spp. and one as achromobacter sp. analysis of the clinical findings suggests that although most of these o ...1976957420
bacteria within ovules and seeds.surface-sterilized ovules and seeds of 27 species of plants were cultured in the water of syneresis of a nutrient medium low in agar content. bacteria were obtained from 30% of the ovules, 15% of the seeds of herbaceous plants, 16% of the seeds of woody plants, 5.4% of the overwintered noncereal seeds, and 13.5% of overwintered cereal seeds. in no instance did every ovule or seed of a plant species contain bacteria. no bacteria were obtained from the hard, waxy seeds of mimosa or yellowwood. the ...1976984839
[survival of epiphytic microorganisms following sublimation dehydration and storage].epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...19761004279
[effect of nitrogen sources on growth and cholesterol decomposing activity of mycobacterium rubrum and achromobacter canadicans].the effect of 12 sources of nitrogen on growth and cholesterine-decomposing activity was studied with mycobacterium rubrum and achromobacter candicans. the yield of biomass and the rate of cholesterine decomposition depended on the source of nitrogen and its concentration in the medium. the highest specific activity of the enzyme decomposing cholesterine was found during growth of the cultures on media containing reduced forms of nitrogen. the activity of the enzyme of mycobacterium rubrum was b ...19761012055
[culture and differentiation of obligatory aerobic gram-negative rods from human material; a scheme for application in routine diagnosis (author's transl)].the diagnosis of obligately aerobic gram-negative rods in the clinical laboratory may encounter difficulties since media used for enterobacteriacae are only partially usable for the diagnosis of this group of bacteria (psuedomonas, xanthomonas, alcaligenes, achromobacter, brucella, bordetella, flavobacterium, moraxella, acinetobacter, and some still unnamed taxa). we have developed a diagnostic scheme, based on recent publications in the field and representing an extension of earlier tables from ...19761015032
unstable generalized transduction in achromobacter.six auxotrophic markers of a halotolerant collagenolytic strain of achromobacter were transduced by four alpha hages. abortive transduction was also demonstrated. the generalized transduction system is unusual as the transductants were unstable, characteristic of transduction by lysogeny. the achromobacter strain is a cryptic lysogen for alpha and purified transductants were either sensitive or resistant to alpha. purified clones from four resistant transductants and one sensitive transductant l ...19751151337
[decomposition of cholesterol by achromobacter candicans].the decomposition of cholesterol by the cell suspensions of achromobacter candicans 42 and by the cell-free extracts of this bacterium was studied. the decomposition of cholesterol in the presence of the wet biomass during four hours of the incubation was 72.5 percent, and with the cells that were preliminarily lyophilized or treated by acetone 49.8 and 23.0 percent, respectively. the activity decreased from 70.0 to 50.0 percent if the biomass was kept at minus 3 degrees c during 70 days. the de ...19751160640
characterization of radiation-resistant vegetative bacteria in beef.ground beef contains numerous microorganisms of various types. the commonly recognized bacteria are associated with current problems of spoilage. irradiation, however, contributes a new factor through selective destruction of the microflora. the residual microorganisms surviving a nonsterilizing dose are predominantly gram-negative coccobacilli. various classifications have been given, e.g., moraxella, acinetobacter, achromobacter, etc. for a more detailed study of these radiation-resistant bact ...19751164011
[on the taxonomy and physiology of bacteria utilizing hydrocarbons in the sea (author's transl)].using mineral media with gas oil as sole carbon source, 191 bacterial strains were isolated from the costal area of marseille. these strains were attributed to achromobacter, alcaligenes, pseudomonas, acinetobacter and arthrobacter genera. amongst isolated strains there was a predominance of the alcaligenes-achromobacter group over others genera. growth and respiratory activity of 5 strains were studied on hexadecane and acetate. respiratory activity on hexadecane of 32 strains cultured on aceta ...19751190640
effect of heat-killed cells of achromobacter nematophilus poinar et thomas, and the fraction (endotoxin) isolated from them on galleria mellonella l. caterpillars. 19751192248
decreased dissociation of the 3-methylcrotonyl-coa carboxylase complex from achromobacter in the presence of 3-methylcrotonyl-coa. a possible regulatory mechanism for the intracellular degradation of the enzyme.by inactivation of different concentrations of 3-methylcrotonyl-coa carboxylase from achromobacter ivs with a fixed concentration of iodoacetamide, it was demonstrated that the degree of dissociation of the complex is considerably lower in the presence of 3-methylcrotonyl-coa. atp did not produce this effect. this property could serve to regulate the intracellular degradation of the enzyme, if the dissociated subunits were attacked preferentially.19751204640
evidence that the type 2 copper centers are the site of nitrite reduction by achromobacter cycloclastes nitrite reductase.methods have been developed for selective depletion and reconstitution of the type 2 cu (non-blue) sites in the nitrite reductase from a. cycloclastes, resulting in preparations ranging from 0.5 to 2.6 type cu per trimer; the type 1 cu content is invariant at 3.0 per trimer. the activity of the enzyme is directly proportional to the type 2 content as measured by direct metal determination or by analysis of the epr spectra. these results indicate that an earlier report that the a. cycloclastes en ...19921329738
cloning and expression of a chick liver glutathione s-transferase cl 3 subunit with the use of a baculovirus expression system.glutathione s-transferase cl 3 subunits purified from 1-day-old-chick livers were digested with achromobacter proteinase i and the resulting fragments were isolated for amino acid sequence analysis. an oligonucleotide probe was constructed accordingly for cdna library screening. a cdna clone of 1342 bases, pgcl301, encoding a protein of 26209 da was isolated and sequenced. including conservative substitutions, this protein has 75-79% sequence similarity to other alpha family glutathione s-transf ...19921339283
limited proteolysis of silkworm antitrypsin by several proteinases.silkworm antitrypsin (sw-at) isolated from larval hemolymph was limitedly digested by achromobacter lysylendopeptidase, alpha-chymotrypsin, subtilisin bpn', subtilisin carlsberg, papain, or pseudomonas elastase. each proteinase could cleave specific site(s) around the reactive site identified for the reaction of sw-at and bovine trypsin. among these proteinases, only subtilisin bpn' was inhibited by sw-at, although weakly. by the cleavable amino acid sequence in sw-at, it was suggested that whet ...19901368515
inhibition of achromobacter protease i by lysinal derivatives.z-val-, z-pro-, z-leu-leu-, and z-leu-pro-lysinals and bz-dl-lysinal were chemically synthesized and tested as novel inhibitors for achromobacter protease i (api), a lysine-specific serine protease. among the lysinal derivatives tested, z-val-lysinal was the most potent competitive inhibitor, its ki being estimated as 6.5 nm in an esterolytic assay with tos-lys-ome. in an amidolytic assay, z-leu-leu-lysinal was the most potent inhibitor and the apparent mode of inhibition was non-competitive. th ...19921369061
prosthetic knee infection due to achromobacter xylosoxidans.achromobacter xylosoxidans is an aerobic gram negative organism that has been infrequently implicated in clinical infections in a variety of anatomical sites. we describe a case of a prosthetic knee infection due to achromobacter xylosoxidans in a patient with rheumatoid arthritis receiving high dose prednisone.19921404142
sequence determination and characterization of a phospholipase a2 isozyme from trimeresurus gramineus (green habu snake) venom.in addition to phospholipase a2-i (pla2-i) reported previously (oda et al., 1991, toxicon 29, 157), a new pla2 named pla2-ii was isolated from trimeresurus gramineus (green habu snake) venom, and its amino acid sequence was determined by sequencing the native protein and the peptides produced by enzymatic (achromobacter protease i and clostripain) cleavages of the carboxamidomethylated derivative of the protein. the protein consisted of 122 amino acid residues and his-47, asp-48, and asp-98 whic ...19921485333
molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.catechol 2,3-dioxygenases were cloned from alcaligenes sp. kf711, pseudomonas putida kf715, and achromobacter xylosoxidans kf701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. all of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (page). the purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. the catechol 2,3-dioxygenase from alcaligenes sp. kf711 exhibit ...19921530619
characterization of catechol 2,3-dioxygenases.three catechol 2,3-dioxygenases for biphenyl, naphthalene/salicylate, and toluene/xylene oxidation were cloned from achromobacter xylosoxidans kf701, pseudomonas putida (nah7), and pseudomonas sp. (pwwo). the cloned catechol 2,3-dioxygenases were identified by enzymatic activity assay in addition to yellow bands on polyacrylamide gel after electrophoresis and activity staining. all of the cloned catechol 2,3-dioxygenases exhibited their highest activities on catechol as a substrate compared with ...19921543511
bacteremia due to achromobacter xylosoxidans in patients with cancer.bacteremia due to achromobacter xylosoxidans is rare, and little information on treatment is available. between 1983 and 1988, a. xylosoxidans was recovered from 26 cultures of blood from 10 patients with cancer and clinical signs of infection, including one patient with septic shock and two with pneumonia. neutropenia did not seem to be a predisposing factor. the infection may have been catheter related in four patients and associated with gastrointestinal pathology in four others. probable cau ...19921554834
catheter-associated sepsis caused by ochrobactrum anthropi: report of a case and review of related nonfermentative bacteria.ochrobactrum anthropi, formerly known as cdc group vd, is an oxidase-producing, gram-negative, non-lactose-fermenting bacillus that oxidizes glucose and grows readily on macconkey agar. only occasionally isolated from human clinical specimens, this organism has rarely been found to be pathogenic. we describe the first reported case of infection due to o. anthropi in a child, that of bacteremia in a 3-year-old girl undergoing chemotherapy for retinoblastoma. in addition, we review the literature ...19921576286
spectral properties of achromobacter xylosoxidans cytochromes c' and their no complexes.cytochromes c' have been isolated from six strains of achromobacter xylosoxidans: ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, 1048, 1051, 1055 and 1764. they are dimeric proteins with more positive redox potentials than those of cytochromes c' from phototrophic bacteria at neutral ph. the electronic absorption, epr and mcd spectra on no-ferrous cytochromes c' at physiological ph showed that the major part of the heme-iron of nitrosylheme was penta-coordinated. the epr spectral re ...19911646026
proton and tritium nmr relaxation studies of peptide inhibitor binding to bacterial collagenase: conformation and dynamics.the interaction of succinyl-pro-ala, a competitive inhibitor of achromobacter iophagus collagenase, with the enzyme was studied by longitudinal proton and tritium relaxation. specific deuterium and tritium labeling of the succinyl part at vicinal positions allowed the measurement of the cross-relaxation rates of individual proton or tritium spin pairs in the inhibitor-enzyme complex as well as in the free inhibitor. overall correlation times, internuclear distances, and qualitative information o ...19911651124
[structure and function of cytochrome c' from achromobacter xylosoxidans]. 19911665239
achromobacter xylosoxidans infection in baboons. 19911666159
puncture wound-induced achromobacter xylosoxidans osteomyelitis of the foot. 19911674638
detection and identification of groundwater bacteria capable of escaping entrapment on 0.45-micron-pore-size membrane filters.rural drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of escaping entrapment on standard porosity (0.45-micron-pore-size) membrane filters. filterable bacteria were present in 42% of the 24 groundwater sources examined by using nonselective media (r2a, full strength m-hpc, and 0.1x m-hpc agars). pseudomonads were the most frequently identified group of filterable bacteria detected. flavobacteri ...19911768096
purification and some properties of phospholipase c from achromobacter xylosoxidans.a non-haemolytic phospholipase c (ec 3.1.4.3) was purified from the culture medium of achromobacter xylosoxidans with a 5% yield and a purification factor of 330. a combination of ultrafiltration, acetone precipitation and two subsequent affinity chromatographic steps was used. the affinity chromatography is a new application of 2-(4-aminophenylsulphonyl)ethyl-cellulose, a sorbent that has previously been used for the purification of phospholipase c from bacillus cereus. the purified enzyme gave ...19911783637
[susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ...19911798067
beta-d-glucuronidase (bdg) activity of gram-negative bacteria.bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ...19911817425
[achromobacter xylosoxidans septicemia]. 19911827195
[an uncommon germ of meningitis, achromobacter xylosoxidans]. 19911829828
amino acid sequence of nitrite reductase: a copper protein from achromobacter cycloclastes.the amino acid sequence of the copper-containing nitrite reductase (ec 1.7.99.3) from achromobacter cycloclastes strain iam 1013 has been determined by using peptides derived from digestion with achromobacter protease i (lys), staphylococcus aureus v8 protease (glu), cyanogen bromide, and bnps-skatole in acetic acid. the subunit contains 340 amino acids. the identity of the first seven amino acids is tentative. the sequence has been instrumental in the x-ray structure determination of this molec ...19911830217
[the dna nucleotide composition of bacteria oxidizing diethylene glycols].nucleotide composition of dna has been analyzed in bacteria composing the diethylene glycol-oxidizing association. it is shown that the microorganisms under study are representatives of genera alkaligenes and achromobacter.19911861652
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