| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from helicobacter pylori by using a monoclonal antibody. | immunoblotting experiments on hyperimmune rabbit serum and sera from patients with helicobacter pylori gastritis showed a consistent antibody response to a 19-kda outer membrane protein antigen. a monoclonal antibody, designated hp 40, which reacted by western immunoblotting with this protein was produced. it was shared by all h. pylori strains tested (d 273, nctc 11637, and 24 wild strains) but not by the thermophilic campylobacter species, campylobacter fetus, helicobacter mustellae, or helico ... | 1991 | 1761682 |
| reassessment of selective agars and filtration techniques for isolation of campylobacter species from faeces. | four different studies were conducted in order to re-evaluate conventional methods and assess the efficacy of new selective agars and a filtration method for the isolation of campylobacters. skirrow's medium, preston agar, modified ccd agar and fennell's medium, incubated microaerobically at 37 degrees c for 48 h, gave similar campylobacter isolation rates from 225 faecal samples, but the latter two media were more selective. evaluation of modified ccd agar demonstrated that campylobacters could ... | 1988 | 3134202 |
| novel campylobacter-like organism resembling helicobacter fennelliae isolated from a boy with gastroenteritis and from dogs. | we isolated a campylobacter-like organism resembling helicobacter fennelliae from a 5 1/2-year-old boy with gastroenteritis. similar strains had been found previously in fecal specimens from healthy and diarrheic dogs. these isolates could be differentiated from h. fennelliae by a lack of catalase and arylsulfatase activities. this group of organisms seems to be homogeneous by a nonradioactive dot blot dna hybridization assay. | 1993 | 8349774 |
| three supplementary diagnostic tests for campylobacter species and related organisms. | we analyzed 307 recent clinical isolates and reference strains of campylobacter spp., helicobacter spp., and arcobacter spp. for arylsulfatase and pyrazinamidase activities and susceptibility to polymyxin b. all isolates studied were identified by dna dot hybridization prior to testing. campylobacter concisus, c. sputorum, and helicobacter fennelliae were positive for arylsulfatase. c. jejuni, c. coli, c. lari, c. upsaliensis, and c. concisus were positive for pyrazinamidase. only helicobacter s ... | 1993 | 8458969 |
| microtiter assay for detecting campylobacter spp. and helicobacter pylori with surface gangliosides which bind cholera toxin. | campylobacter jejuni with gm1 ganglioside in the core of its lipopolysaccharide has been associated with guillain-barré syndrome. since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of gm1 on their surfaces was developed. in the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a ... | 1998 | 9650959 |
| rapid identification of thermotolerant campylobacter jejuni, campylobacter coli, campylobacter lari, and campylobacter upsaliensis from various geographic locations by a gtpase-based pcr-reverse hybridization assay. | recently, a gene from campylobacter jejuni encoding a putative gtpase was identified. based on two semiconserved gtp-binding sites encoded within this gene, pcr primers were selected that allow amplification of a 153-bp fragment from c. jejuni, c. coli, c. lari, and c. upsaliensis. sequence analysis of these pcr products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant campylobacter species. multiple probes we ... | 1999 | 10325325 |
| septic shock due to helicobacter fennelliae in a non-human immunodeficiency virus-infected heterosexual patient. | helicobacter fennelliae (formerly campylobacter fennelliae) has been reported to cause bacteremia in homosexual men with or without human immunodeficiency virus (hiv) infection. we report here a 48-year-old, non-hiv-infected, heterosexual man with diabetes mellitus and cirrhosis of the liver who developed bacteremia and septic shock due to h. fennelliae. the patient was treated successfully initially with intravenous ampicillin-sulbactam and ceftazidime, followed by ampicillin-sulbactam only. th ... | 1999 | 10325388 |
| rapid detection, by pcr and reverse hybridization, of mutations in the helicobacter pylori 23s rrna gene, associated with macrolide resistance. | a pcr-based reverse hybridization system (research prototype kit inno-lipa for h. pylori resistance) was developed and evaluated for simultaneous detection of 23s ribosomal dna point mutations, associated with macrolide resistance in helicobacter pylori. fifty-seven h. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by pcr-lipa (detecting mutations a2115-->g, g2141-->a, a2142-->g, a2142-->c, a2143-->g, a2143-->c, and a2143-->t), d ... | 1999 | 10390244 |
| recurrent bacteremia caused by a "flexispira"-like organism in a patient with x-linked (bruton's) agammaglobulinemia. | helicobacter spp., except for helicobacter cinaedi, have only rarely been reported in cases of septicemia. a patient with x-linked (bruton's) agammaglobulinemia was found to have persistent sepsis with a helicobacter-like organism despite multiple courses of antibiotics. his periods of sepsis were associated with leg swelling thought to be consistent with cellulitis. the organism was fastidious and required a microaerophilic environment containing h(2) for growth. optimal growth was observed at ... | 1999 | 10405381 |
| an uncommon helicobacter isolate from blood: evidence of a group of helicobacter spp. pathogenic in aids patients. | an unusual helicobacter sp. was isolated from the blood of a human immunodeficiency virus (hiv)-infected patient. this organism had spiral morphology, with single amphitrichous flagella, and was negative for hippurate hydrolysis, production of urease, and reduction of nitrate. 16s rrna gene sequence analysis verified that the isolate was a species of helicobacter, most closely related to an undescribed helicobacter-like isolate from vancouver, british columbia, canada, and to helicobacter westme ... | 1999 | 10405434 |
| isolation of helicobacter canis from a colony of bengal cats with endemic diarrhea. | on the basis of biochemical, phenotypic, and 16s rrna analyses, helicobacter canis was isolated from bengal cats with and without chronic diarrhea. because the cats were coinfected with other potential pathogens, including campylobacter helveticus, and because h. canis was isolated from nondiarrheic cats, the causal role of h. canis in producing the diarrhea could not be proven. histologically, the colons of the four affected cats were characterized by mild to moderate neutrophilic, plasmacytic, ... | 1999 | 10488191 |
| rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene. | a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay. | 1999 | 10565952 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| helicobacter mesocricetorum sp. nov., a novel helicobacter isolated from the feces of syrian hamsters. | a spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the feces of syrian hamsters. the bacterium grew as a thin spreading film at 37 degrees c under microaerobic conditions, did not hydrolyze urea, was positive for catalase and alkaline phosphatase, reduced nitrate to nitrite, did not hydrolyze hippurate, and was sensitive to nalidixic acid but resistant to cephalothin. sequence analysis of the 16s rrna gene and biochemical and phenotypic criteria indicate that ... | 2000 | 10790105 |
| application of the 5'-nuclease pcr assay in evaluation and development of methods for quantitative detection of campylobacter jejuni. | campylobacter jejuni is recognized as a leading human food-borne pathogen. traditional diagnostic testing for c. jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable state. nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. in this article, we present a 5'-nuclease pcr assay for quantitative detection of c. jejuni and describe its evaluation. a probe including positions ... | 2000 | 10966425 |
| efficient isolation of campylobacteria from stools. | | 2000 | 10979752 |
| a probable new helicobacter species isolated from a patient with bacteremia. | a probable new helicobacter species was isolated from the blood of a 14-month-old aboriginal child who presented with vomiting, diarrhea, fever, and dry cough. the most similar 16s rrna gene sequence was that of helicobacter fennelliae ccug 18820(t) but the new sequence differed from it by at least 32 base substitutions and by the presence of a large (353-nucleotide) intervening sequence. | 2000 | 11015417 |
| evaluation of the alexon-trend prospect campylobacter microplate assay. | we evaluated stool specimens known to contain or be free of campylobacter by traditional culture, using the prospect campylobacter microplate assay (alexon-trend, ramsey, minn.). this rapid enzyme immunoassay for the detection of campylobacter-specific antigens demonstrated 96% sensitivity and 99% specificity and is an acceptable alternative method of campylobacter detection. | 2000 | 11015419 |
| evaluation of a group-specific 16s ribosomal dna-based pcr for detection of helicobacter bizzozeronii, helicobacter felis, and helicobacter salomonis in fresh and paraffin-embedded gastric biopsy specimens. | a new specific and sensitive 16s ribosomal dna-based pcr assay was developed. the assay targets a 78-bp dna fragment unique to helicobacter bizzozeronii, helicobacter felis, and helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens. | 2001 | 11230459 |
| specific detection and prevalence of helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16s ribosomal dna sequencing. | gastric infection with helicobacter heilmannii (previously known as gastrospirillum hominis) is invariably linked with the presence of chronic gastritis and the risk of developing low-grade mucosa-associated lymphoid tissue lymphoma in humans. in contrast to helicobacter pylori, various h. heilmannii species colonize the stomachs of domestic animals, which might be a reservoir for transmission to humans (zoonosis). to identify the number and prevalence of different h. heilmanni types in humans, ... | 2001 | 11283079 |
| coinfection of enteric helicobacter spp. and campylobacter spp. in cats. | during a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. all the isolates were initially identified as campylobacter-like organisms based on biochemical and phenotypic characteristics. dna extractions from 51 of these isolates were subjected to pcr using primers specific for helicobacter spp. and campylobacter spp. of the isolates, 92% (47 of 51 isolates) were positive for campylobacter spp., 41% (21 of 51 isolates) were positive for helicob ... | 2001 | 11376052 |
| helicobacter winghamensis sp. nov., a novel helicobacter sp. isolated from patients with gastroenteritis. | from 1997 to 1999 seven isolates of campylobacter-like organisms from five patients that were exhibiting symptoms of gastroenteritis, including fever, stomach malaise, and diarrhea, were investigated. the organisms were isolated from stool samples and found to exhibit a diverse colony morphology; hence multiple isolates were submitted from one of the patients. all isolates were found to be identical. the organisms were catalase, urease, alkaline phosphatase, and nitrate negative but oxidase and ... | 2001 | 11427547 |
| efficient isolation of campylobacter upsaliensis from stools. | | 2001 | 11712518 |
| evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque. | a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ... | 2002 | 11773117 |
| human campylobacteriosis in developing countries. | campylobacteriosis is a collective description for infectious diseases caused by members of the bacterial genus campylobacter. the only form of campylobacteriosis of major public health importance is campylobacter enteritis due to c. jejuni and c. coli. research and control efforts on the disease have been conducted more often in developed countries than developing countries. however, because of the increasing incidence, expanding spectrum of infections, potential of hiv-related deaths due to ca ... | 2002 | 11927019 |
| the complete genome sequence of the carcinogenic bacterium helicobacter hepaticus. | helicobacter hepaticus causes chronic hepatitis and liver cancer in mice. it is the prototype enterohepatic helicobacter species and a close relative of helicobacter pylori, also a recognized carcinogen. here we report the complete genome sequence of h. hepaticus atcc51449. h. hepaticus has a circular chromosome of 1,799,146 base pairs, predicted to encode 1,875 proteins. a total of 938, 953, and 821 proteins have orthologs in h. pylori, campylobacter jejuni, and both pathogens, respectively. h. ... | 2003 | 12810954 |
| multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis. | helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ... | 2004 | 15004062 |
| identification of a unique helicobacter species by 16s rrna gene analysis in an abdominal abscess from a patient with x-linked hypogammaglobulinemia. | a unique helicobacter species, mz640285, was isolated from a patient with x-linked hypogammaglobulinemia suffering from recurrent abdominal abscesses and was identified by 16s rrna gene sequence analysis. in the phylogenetic tree, the isolate fell into a cluster which included flexispira rappini, helicobacter bilis, and helicobacter sp. strain mainz. helicobacters are being increasingly recognized as pathogens in immunocompromised hosts. these fastidious bacteria are not easily cultured in the r ... | 2000 | 10878075 |
| prevalence, antimicrobial susceptibility and molecular characterization of campylobacter isolates recovered from humans and poultry in lebanon. | recovery of campylobacter was attempted from 281 consecutive non selected out-patients diarrheic stools, 150 individual ceca collected from meat chicken breeder farms and 31 slaughtered marketed chicken obtained from shops in lebanon. campylobacter isolates were recovered from 2 (0.7%) human stool specimens, 34 (22.7%) chicken ceca and 3 (9.7%) raw chicken carcasses. speciation of these isolates revealed 2 c. jejuni from humans diarrheic stools, 16 c. coli, 10 c. jejuni, 3 c. fetus, 2 c. fennell ... | 1998 | 10349269 |
| recurrent "flexispira rappini" bacteremia in an adult patient undergoing hemodialysis: case report. | a blood culture from a 65-year-old febrile man undergoing hemodialysis revealed, 5 days after inoculation, an unusual gram-negative fusiform rod with darting motility. during another episode of fever 21 days later, this campylobacter-like organism was again recovered from three blood cultures and subcultured under an h2-enriched microaerobic atmosphere. the organism was catalase negative and oxidase positive and hydrolyzed urea rapidly. sodium dodecyl sulfate-polyacrylamide gel electrophoresis a ... | 1999 | 10203478 |
| a novel urease-negative helicobacter species associated with colitis and typhlitis in il-10-deficient mice. | a spiral-shaped bacterium with bipolar, single-sheathed flagella was isolated from the intestines of il-10 (interleukin-10)-deficient (il-10(-/-)) mice with inflammatory bowel disease. the organism was microaerobic, grew at 37 and 42 degrees c, and was oxidase and catalase positive but urease negative. on the basis of 16s rrna gene sequence analysis and biochemical and phenotypic criteria, the organism is classified as a novel helicobacter. cesarean section-rederived il-10(-/-) mice without heli ... | 1999 | 10085015 |
| novel intestinal helicobacter species isolated from cotton-top tamarins (saguinus oedipus) with chronic colitis. | a disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (ctts) in captivity. the clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. approximately 25 to 40% of these animals develop colon cancer after 2 to 5 years of captivity. an infectious etiology has been proposed; however, no microbial agent to date has been identified. helicobacter spp. have been associated ... | 1999 | 9854080 |
| relationship between the severity of hepatitis c virus-related liver disease and the presence of helicobacter species in the liver: a prospective study. | to determine the presence of helicobacter species dna in the liver of chronic hepatitis c (chc) patients with and without cirrhosis as compared to controls, and to identify the bacterial species involved. | 2006 | 17143941 |
| identification of a novel enteric helicobacter species in a kitten with severe diarrhea. | a previously undescribed helicobacter sp. was recovered from a cat with severe diarrhea. based upon the absence of any other identifiable cause of diarrhea, this helicobacter may be involved in the development of the disease signs. the organism could not be cultured but was described on the basis of 16s rrna gene sequence analysis and morphology and appeared to be a new species, with helicobacter canis being the most genetically similar species. the presence of a diarrhea-inducing helicobacter i ... | 1998 | 9542907 |
| evaluation of 16s rrna gene-based pcr assays for genus-level identification of helicobacter species. | the inclusivity, exclusivity, and detection limit of six 16s rrna gene-based helicobacter genus-specific pcr assays were examined. five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). the clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture. | 2008 | 18337392 |
| detection and prevalence of helicobacter infection in pet cats. | the presence of spiral bacteria in the feline stomach has been recognized for over a century, but the identities and degrees of prevalence of such organisms in privately owned cats are still poorly documented. the aims of this study were (i) to adapt different diagnostic tools and evaluate their practicality for diagnosing feline gastric helicobacter colonization, (ii) to determine the prevalence of gastric helicobacter-like organisms in pet cats, (iii) to identify the feline species, and (iv) t ... | 1998 | 9508286 |
| a pathobiont of the microbiota balances host colonization and intestinal inflammation. | the gastrointestinal tract harbors a diverse microbiota that has coevolved with mammalian hosts. though most associations are symbiotic or commensal, some resident bacteria (termed pathobionts) have the potential to cause disease. bacterial type vi secretion systems (t6sss) are one mechanism for forging host-microbial interactions. here we reveal a protective role for the t6ss of helicobacter hepaticus, a gram-negative bacterium of the intestinal microbiota. h. hepaticus mutants with a defective ... | 2010 | 20413095 |
| helicobacter westmeadii sp. nov., a new species isolated from blood cultures of two aids patients. | a slowly growing anaerobic helicobacter species was isolated from the blood cultures of two human immunodeficiency virus-positive patients admitted to westmead hospital, westmead, australia, with fevers. the morphology of the isolates was consistent with helicobacter cinaedi or helicobacter fennelliae. the results of culture growth conditions, biochemical tests, gas chromatography data, ribotyping, and 16s rdna sequencing showed that these isolates represent a new helicobacter species, for which ... | 1997 | 9114397 |
| genotypic and phenotypic characterization of helicobacter cinaedi and helicobacter fennelliae strains isolated from humans and animals. | by dna-dna hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as helicobacter cinaedi as well as 2 human strains and 2 animal strains of helicobacter fennelliae. all but one human strain belonged to the same hybridization group as the type strain of h. cinaedi. the animal strains also appeared to belong to this hybridization group. both human strains of h. fennelliae were shown to be h. fennelliae by dna-dna hybridization, but both a ... | 1995 | 8576350 |
| change is good: variations in common biological mechanisms in the epsilonproteobacterial genera campylobacter and helicobacter. | microbial evolution and subsequent species diversification enable bacterial organisms to perform common biological processes by a variety of means. the epsilonproteobacteria are a diverse class of prokaryotes that thrive in diverse habitats. many of these environmental niches are labeled as extreme, whereas other niches include various sites within human, animal, and insect hosts. some epsilonproteobacteria, such as campylobacter jejuni and helicobacter pylori, are common pathogens of humans tha ... | 2011 | 21372321 |
| campylobacter bacteraemia in england and wales, 1981-91. | routine surveillance of infection in england and wales detected 394 cases of campylobacter bacteraemia in 11 years. this represented an average incidence of 1.5 per 1000 intestinal campylobacter infections, with a range of 0.3/1000 in children aged 1-4 years to 5.9/1000 in patients aged 65 years or more. definitive identification of 257 isolates showed that 89% were campylobacter jejuni or c. coli; other species were c. fetus (8.6%), c. lari (0.8%), c. upsaliensis (0.8%), helicobacter (campyloba ... | 1993 | 8519321 |
| rapid detection of the chlamydiaceae and other families in the order chlamydiales: three pcr tests. | few identification methods will rapidly or specifically detect all bacteria in the order chlamydiales, family chlamydiaceae. in this study, three pcr tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. two tests exclusively recognized the chlamydiaceae: a multiplex test targeting the ompa gene and the rrna intergenic spacer and a taqman test targeting the 23s ribosomal dna. the multiplex test was able to detect as few as 200 inclusion ... | 1999 | 9986815 |
| molecular characterization of the 16s rrna gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in south africa. | forty strains of h. fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in cape town, south africa, were amplified by pcr of the 16s rrna. two distinct genotypes of h. fennelliae were identified based on the phylogenetic analysis. this was confirmed by sequencing a portion of the beta subunit of the rna polymerase (rpob) gene. all isolates from south africa clustered with a proposed novel helicobacter strain (accession number af237612) iso ... | 2010 | 22567323 |
| molecular characterization of the 16s rrna gene of helicobacter fennelliae isolated from stools and blood cultures from paediatric patients in south africa. | forty strains of h. fennelliae collected from paediatric blood and stool samples over an 18 year period at a children's hospital in cape town, south africa, were amplified by pcr of the 16s rrna. two distinct genotypes of h. fennelliae were identified based on the phylogenetic analysis. this was confirmed by sequencing a portion of the beta subunit of the rna polymerase (rpob) gene. all isolates from south africa clustered with a proposed novel helicobacter strain (accession number af237612) iso ... | 2010 | 22567323 |
| isolation and characterization of a novel helicobacter species, helicobacter jaachi sp. nov., from common marmosets (callithrix jaachus). | purpose-bred common marmosets from domestic sources housed in a us research facility, and used in multiple drug discovery programmes, were noted to have a high incidence of spontaneous inflammatory bowel disease and sporadic cholecystitis and cholangiohepatitis. inflammatory infiltrates increased in incidence and severity with age. because helicobacter spp. have been linked to gastrointestinal diseases, samples from the gastrointestinal tracts of 39 marmosets were screened for helicobacter spp. ... | 2015 | 26297446 |
| helicobacter (campylobacter) fennelliae-like organisms as an important but occult cause of bacteraemia in a patient with aids. | we describe the isolation and identification of a helicobacter (campylobacter)-like organism obtained from the blood of a 32-year-old homosexual man with a 10 months' history of aids and progressive mucocutaneous kaposi sarcoma. fever and bacteremia persisted despite sequential administration of ciprofloxacin and trimethoprim-sulfamethoxazole, antibiotics reported to be active against this organism in vitro. facultative organisms like campylobacter fennelliae and campylobacter cinaedi which are ... | 1993 | 8454896 |
| campylobacter cinaedi (sp. nov.) and campylobacter fennelliae (sp. nov.): two new campylobacter species associated with enteric disease in homosexual men. | in a recent study in seattle, we isolated campylobacter-like organisms (clos) significantly more often from homosexual men with intestinal symptoms than from asymptomatic heterosexual and homosexual control subjects. biochemical tests, morphology, and guanine-plus-cytosine content of whole-cell dna showed that these organisms were similar to, but distinguishable from, other species in the genus campylobacter. dna homology tests showed that the campylobacter-like organisms belong to four unique g ... | 1985 | 3965584 |
| correction of the specific epithet in the combination campylobacter fennelliae. | | 1986 | 3944487 |
| successive bacteremias with "campylobacter cinaedi" and "campylobacter fennelliae" in a bisexual male. | a bisexual human immunodeficiency virus-seropositive male had successive bacteremias with "campylobacter cinaedi" and "campylobacter fennelliae." because final identification of both isolates was not completed until 1 month after the last admission of the patient, a novel and nonstandardized antimicrobial susceptibility testing method was useful in guiding timely antimicrobial therapy. | 1987 | 3667922 |
| experimental infection of pig-tailed macaques (macaca nemestrina) with campylobacter cinaedi and campylobacter fennelliae. | campylobacter cinaedi and c. fennelliae have been associated with proctocolitis, bacteremia, and asymptomatic rectal infection, primarily in homosexual men. to more directly assess the pathogenic role of these organisms, we studied their disease-producing potential in 12- to 25-day-old pig-tailed macaques (macaca nemestrina). four infant monkeys were challenged with 10(8) to 10(9) c. cinaedi, three were challenged with c. fennelliae, two were challenged with c. jejuni, and one received no microo ... | 1990 | 2254021 |
| arcobacter species in humans. | during an 8-year study period, arcobacter butzleri was the fourth most common campylobacter-like organism isolated from 67,599 stool specimens. our observations suggest that a. butzleri displays microbiologic and clinical features similar to those of campylobacter jejuni; however, a. butzleri is more frequently associated with a persistent, watery diarrhea. | 2004 | 15504280 |
| glycoconjugate binding of gastric and enterohepatic helicobacter spp. | helicobacter pylori is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. as determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including helicobacter canis, helicobacter hepaticus, and helicobacter bilis, also demonstrated evidence for the presence of lectin-hemagg ... | 2003 | 12704182 |
| captive rhesus monkeys (macaca mulatta) are commonly infected with helicobacter cinaedi. | helicobacter cinaedi may cause proctocolitis or bacteremia in homosexual men infected with human immunodeficiency virus or occasionally in other immunocompromised hosts. there are scattered reports of h. cinaedi isolated from a variety of animal hosts, but to date only hamsters have been found to be a common natural reservoir. microaerophillic cultures of feces from 5 of 16 asymptomatic rhesus monkeys (macaca mulatta) (31%) were positive for a curved gram-negative rod. a polyphasic taxonomic app ... | 2002 | 12037042 |
| isolation of helicobacter cinaedi from the colon, liver, and mesenteric lymph node of a rhesus monkey with chronic colitis and hepatitis. | on the basis of biochemical, phenotypic, and 16s rrna analyses, helicobacter cinaedi was isolated from the colon, liver, and mesenteric lymph nodes of a 2-year-old rhesus monkey with chronic diarrhea. histologically, the liver had mild to moderate biliary hyperplasia and hypertrophy with periportal inflammation and fibrosis. colonic and cecal lesions consisted of diffuse chronic inflammation and glandular hyperplasia extending the length of the crypts. this is the first observation of h. cinaedi ... | 2001 | 11283091 |
| helicobacters of possible zoonotic origin: a review. | since the isolation of helicobacter pylori, many new helicobacter species have been identified from the gastrointestinal tract in humans and animals. in humans, a spiral organism different from h. pylori and provisionally named "helicobacter heilmannii", has been associated with gastritis, gastric ulceration and to a lesser degree, gastric cancer. in addition helicobacter cinaedi, helicobacter fennelliae, helicobacter pullorum and "flexispira rappini" have been isolated from cases of enteric dis ... | 2000 | 11233522 |
| emergence of diverse helicobacter species in the pathogenesis of gastric and enterohepatic diseases. | since helicobacter pylori was first cultivated from human gastric biopsy specimens in 1982, it has become apparent that many related species can often be found colonizing the mucosal surfaces of humans and other animals. these other helicobacter species can be broadly grouped according to whether they colonize the gastric or enterohepatic niche. gastric helicobacter species are widely distributed in mammalian hosts and are often nearly universally prevalent. in many cases they cause an inflammat ... | 2001 | 11148003 |
| helicobacter aurati sp. nov., a urease-positive helicobacter species cultured from gastrointestinal tissues of syrian hamsters. | a novel helicobacter with the proposed name helicobacter aurati (type strain mit 97-5075c) has been isolated from the inflamed stomachs and ceca of adult syrian hamsters. the new species is fusiform with multiple bipolar sheathed flagella and periplasmic fibers; it contains urease and gamma-glutamyl transpeptidase. by 16s rrna sequencing and repetitive element pcr-based dna fingerprinting, it was found that h. aurati represents a distinct taxon and clusters with helicobacter muridarum, helicobac ... | 2000 | 11015391 |
| discordant 16s and 23s rrna gene phylogenies for the genus helicobacter: implications for phylogenetic inference and systematics. | analysis of 16s rrna gene sequences has become the primary method for determining prokaryotic phylogeny. phylogeny is currently the basis for prokaryotic systematics. therefore, the validity of 16s rrna gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. discrepancies between 16s rrna gene analyses and dna-dna hybridization and phenotypic analyses have been noted in the genus helicobacter. to clarify these discrepancies, we sequenced the 23s rrna genes for ... | 2005 | 16109952 |
| misidentifying helicobacters: the helicobacter cinaedi example. | whole-cell protein electrophoresis and biochemical examination by means of a panel of 64 tests were used to identify 14 putative helicobacters to the species level. the results were confirmed by means of dna-dna hybridization experiments and were used to discuss misidentification of helicobacters based on 16s rrna gene sequence data. the data indicated that comparison of near-complete 16s ribosomal dna sequences does not always provide conclusive evidence for species level identification and may ... | 2000 | 10834986 |
| comparison of iron uptake in different helicobacter species. | comparison of iron uptake of four helicobacter species (helicobacter pylori, helicobacter felis, helicobacter acinonyx, and helicobacter mustelae), associated with various degrees of gastritis in their respective host, with five other species which colonize the intestinal tract of various animals (helicobacter fennelliae, helicobacter cinaedi, helicobacter muridarum, helicobacter bilis, and helicobacter hepaticus), demonstrated that the iron acquisition system differed according to the ecologica ... | 1999 | 10540911 |
| inhibitory effect of enterohepatic helicobacter hepaticus on innate immune responses of mouse intestinal epithelial cells. | enterohepatic helicobacter species infect the intestinal tracts and biliary trees of various mammals, including mice and humans, and are associated with chronic inflammatory diseases of the intestine, gallstone formation, and malignant transformation. the recent analysis of the whole genome sequence of the mouse enterohepatic species helicobacter hepaticus allowed us to perform a functional analysis of bacterial factors that may play a role in these diseases. we tested the hypothesis that h. hep ... | 2007 | 17371851 |
| helicobacter marmotae, novel helicobacter sp. and campylobacter sp. isolated from livers and intestines of prairie dogs. | prairie dogs (cynomys ludovicianus) are used to study the etiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. epidemiologic and experimental studies have suggested a connection between helicobacter spp. infection and cholesterol cholelithiasis, cholecystis and gallbladder cancer. ten of thirty four prairie dogs in our study had positive helicobacter spp. identified by pcr with helicobacter genus-specific primers (c97, co5). fourt ... | 2011 | 21546560 |
| helicobacter fennelliae bacteremia: three case reports and literature review. | helicobacter fennelliae is a gram-negative, spiral bacillus that appears as thin-spread colonies on sheep blood agar and is similar to helicobacter cinaedi. h fennelliae is diagnosed by genetic testing, which is not readily available in all laboratories. therefore, h fennelliae bacteremia has only been reported sporadically, and little is known about its clinical characteristics.we describe 3 cases of h fennelliae bacteremia with gastrointestinal symptoms, including nausea, vomiting, and diarrhe ... | 2016 | 27149471 |
| enterohepatic helicobacter species as a potential causative factor in inflammatory bowel disease: a meta-analysis. | the helicobacter species in the gut microbiota comprise helicobacter pylori (h pylori) and enterohepatic helicobacter species (ehs), which can colonize the intestinal mucosa. however, it is unclear whether ehs are associated with inflammatory bowel disease (ibd). therefore, we conducted this meta-analysis to examine the association between ehs and ibd.pubmed, scopus, cochrane library, and web of science databases, as well as abstracts from conference proceedings were searched to identify studies ... | 2015 | 26559250 |
| efficient isolation of campylobacters from stools: what are we missing? | | 2002 | 11896083 |
| recurrent bacteremia with helicobacter cinaedi: case report and review of the literature. | helicobacter cinaedi is a rare pathogen in humans, occurring mostly in immuno-compromised patients, with a high potential for recurrence. we describe a case of a patient with lymphoma hospitalized for chemotherapy. | 2006 | 16719920 |
| persistent infection of rhesus monkeys with 'helicobacter macacae' and its isolation from an animal with intestinal adenocarcinoma. | a novel helicobacter, 'helicobacter macacae', was previously isolated from a colony of rhesus and cynomolgus monkeys in which diarrhoea from chronic idiopathic colitis was enzootic. a survey performed in a second colony of rhesus monkeys without a history of chronic diarrhoea determined that 57 % were faecal-culture positive for helicobacter species. ten years after the survey, one of the animals from which 'h. macacae' had been isolated, a 23-year-old, intact male rhesus monkey (macaca mulatta) ... | 2010 | 20413623 |
| could helicobacter organisms cause inflammatory bowel disease? | the discovery of helicobacter pylori sparked a revolution in the understanding and management of peptic ulcer disease and gastric cancer. other helicobacter species are recognized as important pathogenic agents in colitic diseases of rodents and primates, in particular helicobacter bilis, helicobacter fennelliae, helicobacter hepaticus and helicobacter trogontum. helicobacter bilis and h. hepaticus are now routinely used to initiate rodent models of inflammatory bowel disease (ibd), particularly ... | 2010 | 20955468 |
| diagnosis of genus helicobacter through a hemi-nested pcr assay of 16s rrna. | the present study aimed to establish a genus-specific pcr-based assay to detect helicobacters using 16s rrna gene as the target template. we designed the hemi-nested primers based on sequences of 16s rrna gene of 34 types of helicobacter species. the inclusivity, sensitivity, and specificity of the pcr assay using these primers were examined in three different models, comprising feces simulated samples, blab/c mice infection model and clinic patients samples. the detection sensitivity of helicob ... | 2016 | 27275113 |
| comparing multiplex pcr and rapid urease test in the detection of h. pylori in patients on proton pump inhibitors. | background. this study was conducted to assess the diagnostic value of a multiplex pcr assay to detect h. pylori infection and to further evaluate the negative results from the clotest on patients with and without ppi treatment. methods. this study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at evanston and glenbrook northshore hospital from june 2003 to october 2007. a total of 556 samples were reported with some patients having ... | 2012 | 23319944 |
| a commensal helicobacter sp. of the rodent intestinal flora activates tlr2 and nod1 responses in epithelial cells. | helicobacter spp. represent a proportionately small but significant component of the normal intestinal microflora of animal hosts. several of these intestinal helicobacter spp. are known to induce colitis in mouse models, yet the mechanisms by which these bacteria induce intestinal inflammation are poorly understood. to address this question, we performed in vitro co-culture experiments with mouse and human epithelial cell lines stimulated with a selection of helicobacter spp., including known p ... | 2009 | 19401779 |
| evolution of helicobacter: acquisition by gastric species of two histidine-rich proteins essential for colonization. | metal acquisition and intracellular trafficking are crucial for all cells and metal ions have been recognized as virulence determinants in bacterial pathogens. virulence of the human gastric pathogen helicobacter pylori is dependent on nickel, cofactor of two enzymes essential for in vivo colonization, urease and [nife] hydrogenase. we found that two small paralogous nickel-binding proteins with high content in histidine (hpn and hpn-2) play a central role in maintaining non-toxic intracellular ... | 2015 | 26641249 |
| helicobacter cinaedi cellulitis and bacteremia in immunocompetent hosts after orthopedic surgery. | at various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees c) and local cellulitis at different sites on the operated sides. the wounds had completely healed, without complicated infections, when the cellulitis occurred. the clinical picture of cellulitis in all patients was atypical: diffuse salmon-pink skin color, local heat, swelling, spontaneous pain, and tenderness but no erupti ... | 2007 | 17079500 |
| helicobacter cinaedi cellulitis and bacteremia in immunocompetent hosts after orthopedic surgery. | at various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees c) and local cellulitis at different sites on the operated sides. the wounds had completely healed, without complicated infections, when the cellulitis occurred. the clinical picture of cellulitis in all patients was atypical: diffuse salmon-pink skin color, local heat, swelling, spontaneous pain, and tenderness but no erupti ... | 2007 | 17079500 |
| helicobacter cinaedi and helicobacter fennelliae transmission in a hospital from 2008 to 2012. | forty-six helicobacter cinaedi isolates from the same hospital were analyzed by multilocus sequence typing. most h. cinaedi isolates exhibited clonal complex 9 and were mainly isolated from immunocompromised patients in the same ward. three helicobacter fennelliae isolates were obtained from the same ward and exhibited the same pulsed-field gel electrophoresis patterns. all isolates were resistant to clarithromycin and ciprofloxacin. h. cinaedi and h. fennelliae must be carefully monitored to pr ... | 2013 | 23658263 |
| helicobacter canis bacteremia in a patient with fever of unknown origin. | a 57-year-old woman with common variable immune deficiency and liver failure of unknown etiology presented with recurrent fevers over a 5-month period. she was found to have helicobacter canis bacteremia. immunocompromised hosts with exposure to cats or dogs may be at risk for infection with this organism, which may be challenging to diagnose. | 2013 | 23284025 |
| draft genome sequence of helicobacter fennelliae strain mry12-0050, isolated from a bacteremia patient. | helicobacter fennelliae, a human enterohepatic pathogen, causes bacteremia and colitis. we isolated h. fennelliae strain mry12-0050 from a female patient; this strain was isolated from 2 other patients from the same hospital during the same period, suggesting human-to-human transmission. this is the first report of an h. fennelliae genome sequence. | 2013 | 23929465 |
| microbiological survey of mice (mus musculus) purchased from commercial pet shops in kanagawa and tokyo, japan. | information regarding the prevalence of infectious agents in mice in pet shops in japan is scarce. this information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. therefore, we surveyed infectious agents in mice from pet shops in kanagawa and tokyo, japan. the survey was conducted in 28 mice from 5 pet shops to screen for 47 items (17 viruses, 22 bacteria and fungi, 10 parasites) using culture tests, serology, pcr, and microscopy. the m ... | 2014 | 25502736 |
| microbiological survey of mice (mus musculus) purchased from commercial pet shops in kanagawa and tokyo, japan. | information regarding the prevalence of infectious agents in mice in pet shops in japan is scarce. this information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. therefore, we surveyed infectious agents in mice from pet shops in kanagawa and tokyo, japan. the survey was conducted in 28 mice from 5 pet shops to screen for 47 items (17 viruses, 22 bacteria and fungi, 10 parasites) using culture tests, serology, pcr, and microscopy. the m ... | 2014 | 25502736 |
| pathogenic properties of enterohepatic helicobacter spp. isolated from rhesus macaques with intestinal adenocarcinoma. | considerable progress has been made in understanding the roles of helicobacter pylori in inflammation and gastric cancer; however, far less is known about the roles of enterohepatic helicobacter species (ehs) in carcinogenesis and their zoonotic or pathogenic potential. we determined the prevalence of ehs infection in a cohort of geriatric rhesus monkeys in which intestinal adenocarcinoma (iac) is common and investigated the association between ehs infection and iac. the cohort consisted of 36 a ... | 2014 | 24696515 |
| helicobacter pylori infection and inflammatory bowel disease: is there a link? | helicobacter pylori (h. pylori) infection is one of the most widely spread infectious diseases in humans. it can cause chronic gastritis, peptic ulcer disease and gastric malignancies and has been associated with extra-gastric disorders. h. pylori elicit a chronic systemic inflammatory response which, under certain conditions, may trigger autoimmune reactions and may be implicated in the pathogenesis of autoimmune diseases. although the pathogenesis of inflammatory bowel disease (ibd) is unknown ... | 2014 | 24914359 |
| dietary gluten-induced gut dysbiosis is accompanied by selective upregulation of micrornas with intestinal tight junction and bacteria-binding motifs in rhesus macaque model of celiac disease. | the composition of the gut microbiome reflects the overall health status of the host. in this study, stool samples representing the gut microbiomes from 6 gluten-sensitive (gs) captive juvenile rhesus macaques were compared with those from 6 healthy, age- and diet-matched peers. a total of 48 samples representing both groups were studied using v4 16s rrna gene dna analysis. samples from gs macaques were further characterized based on type of diet administered: conventional monkey chow, i.e., whe ... | 2016 | 27801835 |
| correction for rimbara et al., draft genome sequence of helicobacter fennelliae strain mry12-0050, isolated from a bacteremia patient. | | 2016 | 27445369 |
| first case report of bacteremia due to 'campylobacter-like organism 3'. | a case of bacteremia caused by a rare helicobacter species, campylobacter-like organism 3 (clo-3), in a 75-year-old man with prostate cancer and an indwelling urethral catheter for urinary retention, is reported. oral levofloxacin (500mg per day) was effective, although the results of antimicrobial susceptibility testing were unknown. non-film-like, small, clear colonies were isolated on blood agar after 72h of microaerobic incubation at 37°c. biochemical testing indicated that the isolates were ... | 2016 | 26639044 |
| successful treatment of recurrent helicobacter fennelliae bacteraemia by selective digestive decontamination with kanamycin in a lung cancer patient receiving chemotherapy. | helicobacter fennelliae is an enterohepatic helicobacter species causing bacteraemia in immunocompromised hosts. only a few cases of recurrent h. fennelliae bacteraemia have been reported in japan and there are no guidelines regarding antimicrobial treatment for h. fennelliae infection. | 2016 | 28348791 |
| rapid identification and subtyping of helicobacter cinaedi strains by intact-cell mass spectrometry profiling with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. | helicobacter cinaedi infection is recognized as an increasingly important emerging disease in humans. although h. cinaedi-like strains have been isolated from a variety of animals, it is difficult to identify particular isolates due to their unusual phenotypic profiles and the limited number of biochemical tests for detecting helicobacters. moreover, analyses of the 16s rrna gene sequences are also limited due to the high levels of similarity among closely related helicobacters. this study was c ... | 2014 | 24153128 |
| helicobacter pylori infection in rural and urban dyspeptic patients from venezuela. | the goal of this work was to assess the helicobacter pylori prevalence in a rural mestizo population and compare it to an urban population from venezuela. the study was performed in gastric juice samples of 71 dyspeptic patients from caracas (urban) and 39 from tucupita (rural), in the orinoco delta region. helicobacter pylori was detected by amplification of 16s rrna, glmm, and urea genes in 55.0% patients from urban and 87.2% from rural populations. caga was found positive in 51% and 62% urban ... | 2015 | 26195456 |