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comparative study of lipopolysaccharides from wolinella recta, w. curva, w. succinogenes and campylobacter sputorum ssp. sputorum.lipopolysaccharides (lps) were extracted from cells of wolinella recta atcc 33238, w. curva atcc 33224, w. succinogenes atcc 29543 and campylobacter sputorum ssp. sputorum a 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. chemical compositions of the purified lps including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. all lps preparations contained a ...19911916228
atypical structure of the 23s ribosomal rna molecule in certain oral bacteria.ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ...19911710629
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov.hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ...19911704793
studies on campylobacter sputorum subspecies mucosalis.catalase-negative vibrios can be isolated in large numbers from the affected intestinal mucosa of pigs suffering from a range of porcine enteropathies in which the mucosa has an adenomatous component. these vibrios cannot be distinguished from strains of campylobacter sputorum subsp. mucosalis. the resemblance between these bacteria strengthens the case already made on morphological evidence that these enteropathies have a common origin. catalase-negative vibrios have also been isolated from the ...1976933148
morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus.in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ...19902195067
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
hydrogen-dependent organisms from the human gingival crevice resembling vibrio succinogenes.twenty-eight strains of microaerophilic, motile, slightly curved gram-negative rods isolated from the gingival crevice of patients with gingivitis were studied. they seemed similar to vibrio sputorum, though eleven strains differed in minor characters from bergey's description under the new name campylobacter sputorum, subspecies sputorum. the oral strains studied appeared to be closely related to several species of the genus campylobacter and to vibrio succinogenes. the oral strains were able t ...1976821391
proliferative haemorrhagic enteropathy in pigs.an outbreak of proliferative haemorrhagic enteropathy (phe) occurred in two epidodes and affected 372 adult pigs in the breeding units of a minimal disease piggery; 186 pigs died. in the initial episode breeding sows and boars of all ages were affected, suggesting infection of a fully susceptible population. animals involved in the first episode of the disease did not show clinical symptoms at a later date and further clinical cases occurred only in animals introduced into the breeding populatio ...1977299966
in vitro activities of 47 antimicrobial agents against three campylobacter spp. from pigs.the in vitro activities of 47 antimicrobial agents against 30 isolates of campylobacter species from pigs were determined by the agar dilution technique. the isolates were obtained from pigs with proliferative enteritis and included 10 strains each of campylobacter coli, campylobacter sputorum subsp. mucosalis, and "campylobacter hyointestinalis gebhart et al." (this name is not on the approved lists). carbadox, furazolidone, nitrofurantoin, gentamicin, and dimetridazole were the most active dru ...19853985597
isolation of campylobacter sputorum biovar sputorum from an axillary abscess.campylobacter sputorum biovar sputorum is a rarely isolated organism, particularly from human clinical specimens. its pathogenic potential is unknown. we present here what we believe to be the first report of this organism being isolated from a clinically significant source, an axillary abscess. to our knowledge, this organism has not been reported previously as one of clinical relevance in the u.k.19921569308
the characterisation of campylobacter sputorum subspecies mucosalis isolated from pigs.a comparison is made between the recognised subspecies of campylobacter sputorum isolated from humans and cattle and the previously unrecognised catalase negative vibrios isolated from porcine intestinal adenomatosis. the characters of the porcine strains warrant their inclusion within the species campylobacter sputorum. differentiation between all three is possible in the laboratory and we propose that, in addition to the recognised subspecies, sputorum and bubulus, the pig strains be accorded ...19751129532
porcine intestinal adenomatosis: a possible relationship with necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy.necrotic enteritis and regional ileitis are recognised causes of sporadic death in pigs. in both conditions, the intestinal mucosa harbours the bacterium, campylobacter sputorum subsp mucosalis, which was first associated with procine intestinal adenomatosis.19751080616
isolation of campylobacter sputorum subsp mucosalis from oral cavity of pigs. 19751189242
improved media for growth and aerotolerance of campylobacter fetus.the microaerophilic nature of campylobacter fetus has complicated its recovery from human and animal sources. in this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of c. fetus, representing each subspecies. brucella agar supplemented with 0.025% each feso4 7h2o, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% o2, 17% o2, and 21% o2, respectively. unsupplemented brucella aga ...1978670386
porcine intestinal adenomatosis associated with serologically distinct campylobacter sputorum subspecies mucosalis.a variant of campylobacter sputorum subspecies mucosalis which is serologically distinct from all strains previously examined is described. it has been isolated from cases of porcine intestinal adenomatosis and necrotic enteritis and in all other characteristics conforms to the description of mucosalis.1977928990
the surface antigens of campylobacter sputorum subspecies mucosalis.campylobacter sputorum subsp mucosalis is the vibrio associated with a group of porcine enteropathies in which it is believed the primary lesion is that of adenomatosis. the surface antigens of mucosalis were investigated and individual factor sera were produced; these were used to examine a range of field strains from adenomatosis, necrotic enteritis, regional ileitis and proliferative haemorrhagic enteropathy. neither these not sequential isolants from the same farm showed any distinctive anti ...1977605305
comparison of campylobacter sputorum subspecies mucosalis strains in pia and phe. 1977601990
the effect of different dissolved oxygen tensions on growth and enzyme activities of campylobacter sputorum subspecies bubulus.campylobacter sputorum subspecies bubulus was grown in batch cultures in which the dissolved oxygen tension (d.o.t) was maintained at various constant levels. at a range of d.o.t. from 0.002 to 0.05 atm, which allowed good growth (mean generation time approximately 1.5 h), l-lactate was preferentially consumed before d-lactate. l-lactate oxidation was accompanied by equimolar acetate production during exponential growth. a value for yl-lactate (g dry weight bacteria per mol l-lactate) of 54 was ...1977599328
microaerophily.objections to previous definitions of "anaerobiosis" and "micro-aerophily" are discussed, and a new definition of "micro-aerophily" is proposed. a survey of physiological characteristics of the micro-aerophilic bacterium campylobacter sputorum spp. bubulus is given, and the concept of "micro-aerophily" is illustrated by the results of a study on the oxygen metabolism and oxygen sensitivity of this bacterium.1979552812
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
proliferative haemorrhagic enteropathy.this study indicates that viable campylobacter sputorum subsp mucosalis are not present or are present in small numbers in the mucosa of pigs dying of proliferative haemorrhagic enteropathy. the changes present in the mucosa are similar to those seen in pigs recovering from adenomatosis and the evidence obtained indicates that the intracellular organisms observed in this condition are indeed mucosalis. the presence of large amounts of iga in the altered tissue of both proliferative haemorrhagic ...1979315603
[association of campylobacter sputorum s.s. sputorum with s. fusiformis: transfer of h2 between species]. 1977282238
superoxide dismutase and hydrogen peroxide formation in campylobacter sputorum subspecies bubulus.cell-free extracts of campylobacter sputorum subspecies bubulus contained superoxide dismutase. the enzyme was located in the cytoplasmic fraction and insensitive to cyanide. after centrifuging a cell-free extract at 144000 x g for 1.5 h the total activity in the supernatant fraction was threefold higher than in the crude cell-free extract. the pellet fraction thus obtained was shown to have a lowering effect on superoxide dismutase activities from different sources in the assay method used here ...1978214049
porcine proliferative enteritis: serological, microbiological and pathological studies from three field epizootics.three outbreaks of porcine proliferative enteritis were evaluated clinically, pathologically, microbiologically and serologically. the disease was characterized by a chronic intermittent diarrhea. pathological lesions included a thickened, turbid ileum with the microscopic appearance of proliferating ileal crypt epithelial cells. comma shaped intracytoplasmic organisms were observed in the apical portions of the proliferating crypt epithelial cells with a warthin-starry silver stain. microbiolog ...19863756677
total viable count and differential count of vibrio (campylobacter) sputorum, fusobacterium nucleatum, selenomonas sputigena, bacteroides ochraceus and veillonella in the inflamed and non inflamed human gingival crevice. 1975129552
localization of hydrogenase and nitrate reductase in campylobacter sputorum subsp. bubulus.campylobacter sputorum subsp. bubulus contained hydrogenase activity after growth with lactate and nitrate and after growth with hydrogen and nitrate. after growth with hydrogen and nitrate a molar growth yield (g dry cells/mol hydrogen) of 5.6 was measured. hydrogenase and nitrate reductase were membrane-bound enzymes. in cells with high hydrogenase activity the----h+/o,----h+/no2- and----h+/no3- values with hydrogen as the electron donor were 3.74, 2.61 and 4.36 respectively. in cells with low ...19846372687
behaviour of campylobacter sputorum subspecies mucosalis in gnotobiotic pigs.gnotobiotic pigs were dosed orally with campylobacter sputorum subspecies mucosalis, either alone, or combined with rotavirus or non-pathogenic escherichia coli and streptococcus bovis to study the behaviour of c s mucosalis in defined conditions, to assess intracellular parasitism of enterocytes by c s mucosalis, and if possible to establish an experimental model of porcine intestinal adenomatosis. c s mucosalis colonised the gut of gnotobiotic pigs, persisting for up to 47 days after infection ...19846087431
immunofluorescent demonstration of campylobacter hyointestinalis and campylobacter sputorum subsp mucosalis in swine intestines with lesions of proliferative enteritis.an indirect fluorescent antibody technique was developed to identify campylobacter spp in lesions of swine proliferative enteritis (spe). rabbit antisera to c hyointestinalis and c sputorum subsp mucosalis were produced. bacterial smears stained by fluorescent antibody test with homologous antisera differentiated c hyointestinalis from subsp mucosalis. ileal frozen sections from 29 pigs with histologic lesions of spe had specific fluorescent staining of c hyointestinalis in all 29 and subsp muco ...19846375483
campylobacter sputorum subsp mucosalis and campylobacter hyointestinalis infections in the intestine of gnotobiotic pigs.at 4 days of age, 7 gnotobiotic pigs were orally inoculated with broth cultures of both campylobacter sputorum subsp mucosalis (csm) and campylobacter hyointestinalis (ch). one pig was killed and evaluated each week for 7 weeks. forty-eight hours after inoculation, ch and csm were recovered from the feces of the pigs; thereafter, only ch was recovered. organisms morphologically typical of campylobacter sp were observed on the mucosal surface and on the crypt epithelial cells of the ileum, cecum, ...19854062022
dna homology studies of the catalase-negative campylobacters and "campylobacter fecalis," an emended description of campylobacter sputorum, and proposal of the neotype strain of campylobacter sputorum.twenty-three strains of catalase-negative campylobacters and five strains of "campylobacter fecalis," which is catalase-positive, were examined by dna hybridization experiments. these organisms formed four distinct dna homology groups corresponding to campylobacter sputorum, campylobacter mucosalis, campylobacter concisus, and a currently unnamed group referred to as the "catalase-negative or weak" (cnw) strains. the strains were further characterized to determine which phenotypic characteristic ...19854084862
genetic relationship between "campylobacter fecalis" and campylobacter sputorum subsp. bubulus.nine strains of "campylobacter fecalis" were compared with two references strains of c. sputorum subsp. bubulus considering dna base composition and dna-dna-hybridization. the results showed that the two taxa could not be separated by these methods, their mol%(g+c) being 31-32 and dna relatedness 79% or more. it is suggested that the catalase-positive "campylobacter fecalis" should be regarded as a subspecies of the catalase-negative c. sputorum.19854083014
isolation of campylobacter sputorum subsp. mucosalis in norway. 19846534167
isoprenoid quinone content and cellular fatty acid composition of campylobacter species.a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ...19846470096
campylobacter hyointestinalis (new species) isolated from swine with lesions of proliferative ileitis.intestines from 48 swine with enteric disease were examined by bacteriologic cultural technique for the presence of various campylobacter species. histopathologic techniques were used to determine whether the submitted specimens had lesions of either swine proliferative ileitis or other enteric diseases. three species of campylobacter were identified as campylobacter jejuni/coli, campylobacter sputorum ss mucosalis, and campylobacter hyointestinalis (proposed new species) on the basis of biochem ...19836838031
nucleic acids in the classification of campylobacters.the importance of campylobacters in human disease has stimulated improvements in the methods for identification of strains from hospitals and the environment. reliable and accurate identification depends on a sound classification for which nucleic acid analyses provide fundamental information about species relationships. studies on the genus campylobacter show that the genome dna of species have base compositions of 29 to 38 mol% g + c and molecular weights of 1.54 x 10(9) to 2.31 x 10(9). campy ...19836194994
presence of serum agglutinins to campylobacter sputorum subspecies mucosalis in pigs.pigs affected with intestinal adenomatosis had agglutinating antibodies to campylobacter sputorum subspecies mucosalis. the titres obtained varied, depending on the strain used as antigen and appeared to rise with the age of the affected animal. examination of sera from experimental animals showed that pigs between 30 and 77 days old rarely had antibody, even when they had been exposed to infection. evidence is also presented indicating that the serological response is specific and that in a lim ...19827045998
identification and characterization of an immunogenic outer membrane protein of campylobacter jejuni.we cloned and expressed in escherichia coli a gene encoding an 18-kda outer membrane protein (omp18) from campylobacter jejuni atcc 29428. the nucleotide sequence of the gene encoding omp18 was determined, and an open reading frame of 165 amino acids was revealed. the amino acid sequence had the typical features of a leader sequence and a signal peptidase ii cleavage site at the n-terminal part of omp18. moreover, the sequence had a high degree of similarity to the peptidoglycan-associated outer ...19958576327
the isolation and prevalence of campylobacters from dairy cattle using a variety of methods.faecal samples from 94 dairy cows and 42 calves in three different herds were examined by a variety of techniques for campylobacters. cefoperazone amphotericin teicoplanin (cat) agar, modified cefoperazone charcoal deoxycholate agar (mccda), karmali agar, and membrane filtration onto blood agar, were used with and without enrichment in cat broth. seventy-nine percent of cattle in herd a carried campylobacters, compared with 40% and 37.5% of cattle in herds b and c, respectively. most animals car ...19989674125
degradation of lactoferrin by periodontitis-associated bacteria.the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...19968961558
emended description of campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including c. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans.a polyphasic taxonomic study of 15 bovine and human strains assigned to the catalase-negative, urease-positive campylobacter (cnupc) group identified these bacteria as a novel, ureolytic biovar of campylobacter sputorum for which we propose the name c. sputorum bv. paraureolyticus: suitable reference strains are lmg 11764 (human isolate) and lmg 17590 (= ccug 37579, bovine isolate). the present study confirmed previous findings showing that the salient biochemical tests used to differentiate c. ...19989542089
porcine proliferative enteritis: experimentally induced disease in cesarean-derived colostrum-deprived pigs.the hypotheses that porcine proliferative enteritis is an infectious disease and that campylobacter sputorum subsp mucosalis (csm) is involved in the development of this disease were experimentally tested. three experiments were conducted with 10-week-old, cesarean-derived colostrum-deprived pigs. of 22 pigs given homogenized mucosal scrapings (crude inocula) intragastrically, 15 had gross and/or microscopic lesions of proliferative enteritis. of 10 pigs inoculated with cultures of both csm and ...19827149408
naturally occurring porcine proliferative enteritis: pathologic and bacteriologic findings.the lesions of porcine proliferative enteritis were studied by light and electron microscopic techniques in feeder pigs, fattening hogs, bred gilts, sows, and boars. the characteristic microscopic feature common to all age groups was proliferation of immature crypt epithelial cells, primarily in the ileum and the distal part of the jejunum. similar changes were also observed in the midjejunum, cecum, and colon of a new swine. the earliest detectable microscopic lesion was focal proliferation of ...19827149407
detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples by a seminested pcr assay.a rapid and sensitive assay was developed for detection of small numbers of campylobacter jejuni and campylobacter coli cells in environmental water, sewage, and food samples. water and sewage samples were filtered, and the filters were enriched overnight in a nonselective medium. the enrichment cultures were prepared for pcr by a rapid and simple procedure consisting of centrifugation, proteinase k treatment, and boiling. a seminested pcr based on specific amplification of the intergenic sequen ...199910103261
relationship between c-telopeptide pyridinoline cross-links (ictp) and putative periodontal pathogens in periodontitis.crevicular fluid pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp) is predictive for future alveolar bone loss in experimental periodontitis in dogs. the present study sought to relate ictp to a panel of subgingival species in subjects exhibiting various clinical presentations such as health (n=7), gingivitis (n=8) and periodontitis (n=21). 28 subgingival plaque and gcf samples were taken from mesiobuccal sites in each of 36 subjects. the presence and levels of 40 s ...19989846794
natural infection of the oral cavity of young piglets with campylobacter sputorum ssp mucosalis. 19817303446
isolation of campylobacter sputorum ssp. mucosalis from proliferative hemorrhagic enteropathy in pig. 19826982993
growth yields and energy generation by campylobacter sputorum subspecies bubulus during growth in continuous culture with different hydrogen acceptors. 19807369827
the experimental infection of neonatal pigs with campylobacter sputorum subspecies mucosalis.pigs dosed orally with campylobacter sputorum subsp mucosalis in the neonatal period became infected and mucosalis was recovered from the intestinal mucosa for up to 40 days after infection. rapid spread to undosed litter mates occurred. gross adenomatous change did not develop.19807414059
experimentally induced porcine proliferative enteritis in specific-pathogen-free pigs.thirty-three, 10-week-old, specific-pathogen-free pigs were randomly allotted to 3 treatment groups: group 1--intragastrically given homogenized intestinal mucosa (crude inoculum) from pigs with naturally occurring proliferative enteritis; group 2--given cultures of campylobacter sputorum subsp mucosalis; and group 3--controls. one pig from each group was killed 4, 7, 10, 14, 18, 21, 24, 28, 31, 36, and 38 days after inoculation. the earliest intestinal lesion observed in groups 1 and 2 was leuk ...19826216836
immunisation of pigs with campylobacter sputorum subspecies mucosalis vaccine. 19807257110
the experimental infection of pigs with campylobacter sputorum subspecies mucosalis. weaned pigs, with special reference to pharmacologically mediated hypomotility.the weaned pig proved resistant to infection with campylobacter sputorum subsp mucosalis after oral dosing. pretreatment with benzetimide to reduce gastrointestinal peristalsis allowed mucosalis infection to be established.19807414060
electron transport-linked proton translocation at nitrite reduction in campylobacter sputorum subspecies bubulus.campylobacter sputorum subspecies bubulus contains a membrane-bound nitrite reductase which catalyses the six-electron reduction of nitrite to ammonia. formate and l-lactate are used as hydrogen donors. cells of c. sputorum grown with nitrate or nitrite contain cytochromes of the b- and c-type and a carbon monoxide-binding cytochrome c. in addition, a special membrane-bound carbon monoxide-binding pigment is found. nitrite reduction with formate or l-lactate as a hydrogen donor is strongly inhib ...19826280634
identification of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, arcobacter butzleri, and a. butzleri-like species based on the glya gene.currently, the detection and identification of campylobacter and arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. we have developed a pcr-hybridization strategy, wherein degenerate primers are used to amplify glya fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between campylobacter jejuni, c. coli, c. ...200010747131
oxygen consumption by campylobacter sputorum subspecies bubulus with formate as substrate.the kinetics of oxygen utilization by the microaerophile campylobacter sputorium subspecies bubulus was studied. with formate as substrate two enzyme systems were found to be responsible for electron transfer between formate and oxygen. in the case of lactate oxidation one enzyme system could account for the activity measured. one of the formate-oxidizing systems possessed a high affinity for oxygen [km(o2) = approx. 4 microm o2]. from inhibitor studies it was concluded that a respiratory chain ...19807425784
porcine intestinal adenomatosis: epithelial dysplasia and infiltration.occasionally in cases of porcine intestinal adenomatosis (pia) epithelial dysplasia is seen with infiltration of the epithelium into underlying tissues and spread, via the lymphatics, to drainage lymph nodes. the intracellular bacterium campylobacter sputorum subspecies mucosalis, associated with pia, can be demonstrated in the epithelial cells of the metastases. this dysplasia and infiltration appears to be related to surface damage and inflammation.19807461463
helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis.four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ...19938379970
molecular cloning and site-specific mutagenesis of a gene involved in arylsulfatase production in campylobacter jejuni.the arylsulfatase gene from campylobacter jejuni 81-176 encodes a predicted protein of 69,293 da which shows no sequence similarity with other known arylsulfatases. the gene hybridizes to other ast+ strains of c. jejuni and campylobacter sputorum subsp. bubulus, as well as to many ast- strains of c. jejuni.19968655516
rapid detection of campylobacter jejuni in stool specimens by an enzyme immunoassay and surveillance for campylobacter upsaliensis in the greater salt lake city area.the alexon-trend, inc. (ramsey, minn.), prospect campylobacter microplate assay was compared with culture on a campy-cva plate (remel, lenexa, kans.) and blood-free campylobacter agar with cefoperazone (20 microg/ml), amphotericin b (10 microg/ml), and teicoplanin (4 microg/ml) (cat medium; oxoid limited, hampshire, england) with 631 patient stool samples. the cat medium was used to isolate campylobacter upsaliensis. the enzyme immunoassay (eia) had a sensitivity and a specificity of 89 and 99%, ...200010921981
misidentifying helicobacters: the helicobacter cinaedi example.whole-cell protein electrophoresis and biochemical examination by means of a panel of 64 tests were used to identify 14 putative helicobacters to the species level. the results were confirmed by means of dna-dna hybridization experiments and were used to discuss misidentification of helicobacters based on 16s rrna gene sequence data. the data indicated that comparison of near-complete 16s ribosomal dna sequences does not always provide conclusive evidence for species level identification and may ...200010834986
recommendations of the subcommittee on the taxonomy of campylobacter and related bacteria.the icsb subcommittee on the taxonomy of campylobacter and related bacteria has discussed several contemporaneous issues and makes the following recommendations. (i) the reported synonymy between campylobacter coli and campylobacter hyoilei was based on valid taxonomic arguments. the subcommittee therefore discourages the use of the name c. hyoilei. (ii) the revised infrasubspecific nomenclature of campylobacter sputorum is endorsed. c sputorum is subdivided into c. sputorum biovar sputorum (cha ...200111321120
[isolation of campylobacter sputorum biovar sputorum from a bed sore]. 19979147509
campylobacter hominis sp. nov., from the human gastrointestinal tract.sequences of 16s rdna of a novel campylobacter from faeces of healthy humans were previously shown to originate from a new taxon, 'candidatus campylobacter hominis', which could not be cultured. since phylogenetic analysis suggested that anaerobic conditions might be required for growth, an isolation strategy was developed employing initial non-selective membrane filtration onto fastidious anaerobe agar. campylobacters were then isolated from the resulting mixed microbial flora by a dilution str ...200111321111
isolation and identification of helicobacter spp. from canine and feline gastric mucosa.it is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa. three species, helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. the aims of this study were to evaluate the efficacy of an isolation method for canine and feline gastric helicobacters that has been developed at the university of helsinki; to estimate the prevalence and distribution of these t ...19989758832
bacteremia due to campylobacter sputorum biovar sputorum. 19989868686
clonality of campylobacter sputorum bv. paraureolyticus determined by macrorestriction profiling and biotyping, and evidence for long-term persistent infection in cattle.eighteen strains of campylobacter sputorum bv. paraureolyticus (isolated over a 12-month period from seven dairy cows contained in a single herd) were examined by resistotyping, and macrorestriction profiling using pulsed field gel electrophoresis (pfge). the resistotypes of these strains were identical, although repeat testing indicated resistance to metronidazole was not a reliable trait for typing purposes. five smai-derived genotypes were identified among the 18 strains. in 5 of 7 cows, isol ...199910098802
prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters.the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ...200010618103
differentiation of campylobacter species by aflp fingerprinting.the fluorescent amplified fragment length polymorphism (aflp) fingerprinting method was tested for its ability to identify and subtype the most important campylobacter species found in veterinary infections. sixty-nine reference strains and 19 clinical isolates of campylobacter jejuni subsp. jejuni, campylobacter jejuni subsp. doylei, campylobacter upsaliensis, campylobacter coli, campylobacter lari, campylobacter fetus subsp. fetus, c. fetus subsp. venerealis, campylobacter hyointestinalis subs ...200111577152
rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene.a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay.199910565952
isolation of campylobacter sputorum from lesions of papillomatous digital dermatitis in dairy cattle. 199910515621
comparison of two methods for serotyping campylobacter spp.two serotyping schemes (penner and laboratory of enteric pathogens [lep]) based on soluble heat-stable antigens were used to analyze 3,788 campylobacter sp. isolates. a significant percentage (36.6%) was untypeable using lep serotyping; greater cross-reaction was also observed. the relative discrimination capabilities of the techniques were similar. penner serotyping fulfils more of the requisite criteria for typing methods.200111326013
agglutinins to bacteria in crohn's disease.sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ...19807429299
establishment of human indigenous bacteria in germ-free mice.gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ...196414234787
multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis.helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ...200415004062
direct quantification of campylobacter jejuni and campylobacter lanienae in feces of cattle by real-time quantitative pcr.campylobacter species are fastidious to culture, and the ability to directly quantify biomass in microbiologically complex substrates using real-time quantitative (rtq) pcr may enhance our understanding of their biology and facilitate the development of efficacious mitigation strategies. this study reports the use of nested rtq-pcr to directly quantify campylobacter jejuni and campylobacter lanienae in cattle feces. for c. jejuni, the single-copy mapa gene was selected. for c. lanienae, the thre ...200415066825
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type.the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ...200717172349
oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type.the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ...200717172349
efficient isolation of campylobacteria from stools. 200010979752
differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa.we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ...200415583280
biochemical characteristics of vibrio sputorum and relationship to vibrio bubulus and vibrio fetus.loesche, walter j. (forsyth dental center and harvard school of dental medicine, boston, mass.), ronald j. gibbons, and sigmund s. socransky. biochemical characteristics of vibrio sputorum and relationship to vibrio bubulus and vibrio fetus. j. bacteriol. 89:1109-1116. 1965.-twenty-seven strains of vibrio sputorum were isolated from human gingival debris. all strains reduced nitrate beyond nitrite, and all produced h(2)s. none produced catalase, indole, or acetylmethylcarbinol. carbohydrates wer ...196514276104
identification of taxonomic and epidemiological relationships among campylobacter species by numerical analysis of aflp profiles.amplified fragment length polymorphism (aflp)-based profiling was performed on 138 strains representing all named campylobacter species and subspecies. profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. the mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. species were clearly distinguished at the 17.90% similarity (s-) level in the dendrogram. subspecies of campylobacter jejuni and campylobacter ...200011094296
enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada.the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ...201021106791
rapid identification of campylobacter spp. by melting peak analysis of biprobes in real-time pcr.we describe rapid pcr-biprobe identification of campylobacter spp. this is based on real-time pcr with product analysis in the same system. the assay identifies enteropathogenic campylobacters to the species level on the basis of their degree of hybridization to three 16s ribosomal dna (rdna) biprobes. first-round symmetric pcr is performed with genus-specific primers which selectively target and amplify a portion of the 16s rrna gene common to all campylobacter species. second-round asymmetric ...200111376061
prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds.a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ...200212450810
comparison of genotypes and serotypes of campylobacter jejuni isolated from danish wild mammals and birds and from broiler flocks and humans.the incidence of human infection with campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. environmental sources are believed to be important reservoirs of campylobacter infection in broiler chicken flocks. we investigated the potential importance of wildlife as a source of infection in commercial poultry ...200111425730
c-telopeptide pyridinoline cross-links (ictp) and periodontal pathogens associated with endosseous oral implants.detection of periodontal or peri-implant sites exhibiting progressing disease or those at risk of deterioration has proven difficult. pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp), a marker specific for bone degradation found in gingival crevicular fluid (gcf), has been associated with both bone and attachment loss in periodontitis and may be useful for predicting disease activity. the aim of this cross-sectional study was to examine the relationship between ict ...199811429938
efficient isolation of campylobacter upsaliensis from stools. 200111712518
development and application of a new scheme for typing campylobacter jejuni and campylobacter coli by pcr-based restriction fragment length polymorphism analysis.a molecular typing approach for campylobacter jejuni and campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb pcr-amplified portion of the lipopolysaccharide gene cluster. sixty-one penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. eleven additional genogroups were obtained from 87 clinical c. jejuni strains tested. this molecular typing method shows a correlation with the penner heat-stab ...200211980961
enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada.the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ...201021106791
evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli.we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ...200312517869
campylobacter fetus-associated epidural abscess and bacteremia.campylobacter fetus has been isolated as an infrequent cause of abscesses. we report a case of campylobacter fetus epidural abscess and bacteremia in a debilitated elderly man who had a fatal outcome.200919144805
a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture.a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ...200312620820
campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (pan troglodytes schweinfurthii) in tanzania.the transmission of simian immunodeficiency and ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. in this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (pan troglodytes) populations with different histories in relation to their proximity to humans. campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research ...201121278267
occurrence and characterisation of intervening sequences (ivss) within 16s rrna genes from two atypical campylobacter species, c. sputorum and c. curvus.a polymerase chain reaction (pcr) method was carried out on 21 isolates of atypical campylobacter sputorum (n=14) and c. curvus (n=7) using a primer pair to amplify the helix 11 region within 16s ribosomal rna (rrna) gene sequences. following sequencing and alignment analysis, 14 c. sputorum (100%) and six c. curvus (86%) isolates were shown to carry intervening sequences (ivss) in this region. interestingly, the nucleotide sequences of all the ivss were identical among the 14 c. sputorum isolat ...201020669763
three-hour molecular detection of campylobacter, salmonella, yersinia, and shigella species in feces with accuracy as high as that of culture.campylobacter jejuni and salmonella, shigella, and yersinia species (along with shiga toxin-producing escherichia coli) are the most common causes of acute bacterial diarrheal disease in the united states. current detection techniques are time-consuming, limiting usefulness for patient care. we developed and validated a panel of rapid pcr assays for the detection and identification of c. jejuni, c. coli, salmonella, and yersinia species and shigella and enteroinvasive e. coli in stool samples. a ...201020519461
molecular analysis and characterization of a urease gene operon from campylobacter sputorum biovar paraureolyticus.when recombinant plasmid dna from a genomic dna library and inverse pcr products of campylobacter sputorum biovar paraureolyticus lmg17591 strain were analyzed, an approximate 6.5-kb pair region, encoding a urease gene operon, was identified. within the operon, seven closely spaced and putative open reading frames for ureg, ureh(d), urea, ureb, urec, uree, and uref were detected in order. a possible overlap was detected between ureg and ureh(d), ureh(d) and urea, and uree and uref. in addition, ...201121431911
use of pcr for direct detection of campylobacter species in bovine feces.this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ...200312788747
detection of campylobacter spp. in chicken fecal samples by real-time pcr.a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ...200415528705
urease-positive thermophilic campylobacter species. 200415294767
design and evaluation of useful bacterium-specific pcr primers that amplify genes coding for bacterial 16s rrna.we report the design and evaluation of pcr primers 63f and 1387r for amplification of 16s rrna genes from bacteria. their specificity and efficacy were tested systematically with a bacterial species and environmental samples. they were found to be more useful for 16s rrna gene amplification in ecological and systematic studies than pcr amplimers that are currently more generally used.19989464425
comparison of culture and a novel 5' taq nuclease assay for direct detection of campylobacter fetus subsp. venerealis in clinical specimens from cattle.a campylobacter fetus subsp. venerealis-specific 5' taq nuclease pcr assay using a 3' minor groove binder-dna probe (taqman mgb) was developed based on a subspecies-specific fragment of unknown identity (s. hum, k. quinn, j. brunner, and s. l. on, aust. vet. j. 75:827-831, 1997). the assay specifically detected four c. fetus subsp. venerealis strains with no observed cross-reaction with c. fetus subsp. fetus-related campylobacter species or other bovine venereal microflora. the 5' taq nuclease a ...200616517880
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