| campylobacter showae sp. nov., isolated from the human oral cavity. | nine campylobacter-like strains were isolated from human gingival crevices and characterized. these strains were gram-negative, straight rods that were motile by means of multiple unipolar flagella. they were asaccharolytic and preferred an anaerobic atmosphere rather than a microaerophilic atmosphere for growth, and their growth was stimulated by formate and fumarate. these strains were biochemically similar to campylobacter curvus and campylobacter rectus, but were clearly distinguishable from ... | 1993 | 7694633 |
| recently described clinically important anaerobic bacteria: taxonomic aspects and update. | a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ... | 1997 | 9310640 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| relationship between c-telopeptide pyridinoline cross-links (ictp) and putative periodontal pathogens in periodontitis. | crevicular fluid pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp) is predictive for future alveolar bone loss in experimental periodontitis in dogs. the present study sought to relate ictp to a panel of subgingival species in subjects exhibiting various clinical presentations such as health (n=7), gingivitis (n=8) and periodontitis (n=21). 28 subgingival plaque and gcf samples were taken from mesiobuccal sites in each of 36 subjects. the presence and levels of 40 s ... | 1998 | 9846794 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| campylobacter species in health, gingivitis, and periodontitis. | at least seven campylobacter species have been identified from subgingival sites. campylobacter rectus has been implicated as a periodontal pathogen; however, association with periodontal infections of other campylobacter species, especially the newly described campylobacter showae, is unclear. this study examined which campylobacter species were associated with periodontal health and disease. subgingival campylobacter species from initial and established periodontitis were compared with species ... | 2000 | 10728981 |
| microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients. | polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities. | 2000 | 11073323 |
| c-telopeptide pyridinoline cross-links (ictp) and periodontal pathogens associated with endosseous oral implants. | detection of periodontal or peri-implant sites exhibiting progressing disease or those at risk of deterioration has proven difficult. pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp), a marker specific for bone degradation found in gingival crevicular fluid (gcf), has been associated with both bone and attachment loss in periodontitis and may be useful for predicting disease activity. the aim of this cross-sectional study was to examine the relationship between ict ... | 1998 | 11429938 |
| microbial profile on metallic and ceramic bracket materials. | the placement of orthodontic appliances creates a favorable environment for the accumulation of a microbiota and food residues, which, in time, may cause caries or exacerbate any pre-existing periodontal disease. the purpose of the present study was to compare the total bacterial counts present on metallic and ceramic orthodontic brackets in order to clarify which bracket type has a higher plaque retaining capacity and to determine the levels of streptococcus mutans and lactobacillus spp on both ... | 2002 | 12169034 |
| detection and quantification of oral treponemes in subgingival plaque by real-time pcr. | oral treponemes have been associated with periodontal diseases. we developed a highly sensitive and specific method to detect and quantify cultivable oral treponemes (treponema denticola, treponema vincentii, and treponema medium) in 50 subgingival plaque samples from 13 healthy subjects as well as 37 patients with periodontal diseases using real-time pcr assays with specific primers and a taqman probe for each 16s rrna sequence. the specificity for each assay was examined by using dna specimens ... | 2002 | 12202575 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| detection of campylobacter rectus in periodontitis sites by monoclonal antibodies. | campylobacter rectus, a gram-negative, microaerophilic, and motile bacterium, has been proposed to play a pathogenic role in human periodontitis. surface components, such as the flagellum, surface layer (s-layer), and cytotoxin, have been reported as possible virulence factors of the microorganism. in the present study, monoclonal antibodies against surface components of this bacterium were produced to detect and investigate the pathogenic potential of c. rectus in periodontitis. two monoclonal ... | 2003 | 12558939 |
| clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis. | the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy. | 2004 | 15367191 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| phototargeting oral black-pigmented bacteria. | we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ... | 2005 | 15793117 |
| the salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects. | the purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (oscc) lesion would differ from those found in cancer-free (oscc-free) controls. | 2005 | 15987522 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| biochemical differentiation and comparison of desulfovibrio species and other phenotypically similar genera. | seventeen human clinical isolates representing four species of desulfovibrio were characterized using 16s rrna gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. eighty additional strains representing 10 phenotypically similar genera (bilophila, selenomonas, capnocytophaga, campylobacter, bacteroides, sutterella, anaerobiospirillum, dialister, v ... | 2005 | 16081948 |
| microbial risk indicators of early childhood caries. | the aim of this study was to use molecular identification methods, such as 16s rna gene sequence and reverse-capture checkerboard hybridization, for identification of the bacteria associated with dental caries and with dental health in a subset of 204 twins aged 1.5 to 7 years old. a total of 448 plaque samples (118 collected from caries-free subjects and 330 from caries-active subjects) were used for analysis. we compared the bacteria found in biofilms of children exhibiting severe dental carie ... | 2005 | 16272513 |
| synergistes group organisms of human origin. | the bacterial division synergistes represents a poorly characterized phylotype of which only a few isolates have been cultured, primarily from natural environments. recent detection of synergistes-like sequence types in periodontal pockets and caries lesions of humans prompted us to search the r. m. alden culture collection (santa monica, calif.) for biochemically unidentifiable, slow-growing, obligately anaerobic gram-negative bacilli. here we report on five clinical isolates cultured from peri ... | 2006 | 16891512 |
| changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16s cloning and sequencing. | the gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. the objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status u ... | 2006 | 17021095 |
| molecular analysis of oral and respiratory bacterial species associated with ventilator-associated pneumonia. | trauma intensive care unit (ticu) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (vap). oral and oropharyngeal bacteria are believed to be responsible for many cases of vap, but definitive evidence of this relationship is lacking. earlier studies used conventional culture-based methods for identification of bacterial pathogens, but these methods are insufficient, as some bacteria may be uncultivable or difficult to grow. the purpose of this s ... | 2007 | 17301280 |
| bacteria of dental caries in primary and permanent teeth in children and young adults. | although streptococcus mutans has been implicated as a major etiological agent of dental caries, our cross-sectional preliminary study indicated that 10% of subjects with rampant caries in permanent teeth do not have detectable levels of s. mutans. our aims were to use molecular methods to detect all bacterial species associated with caries in primary and permanent teeth and to determine the bacterial profiles associated with different disease states. plaque was collected from 39 healthy control ... | 2008 | 18216213 |
| factors affecting human supragingival biofilm composition. ii. tooth position. | little is known regarding the factors that affect the microbial composition of supragingival biofilms. this study was designed to test the hypothesis that tooth location affects the microbial composition of supragingival plaque beyond the effect due to plaque mass as reflected by total dna probe count. | 2009 | 18973539 |
| factors affecting human supragingival biofilm composition. i. plaque mass. | little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. | 2009 | 18973540 |
| microarray analysis of the microflora of root caries in elderly. | the present study used a new 16s rrna-based microarray with probes for over 300 bacterial species to better define the bacterial profiles of healthy root surfaces and root caries (rc) in the elderly. supragingival plaque was collected from 20 healthy subjects (controls) and from healthy and carious roots and carious dentin from 21 rc subjects (patients). collectively, 179 bacterial species and species groups were detected. a higher bacterial diversity was observed in controls than in patients. l ... | 2009 | 19039610 |
| microarray analysis of the microflora of root caries in elderly. | the present study used a new 16s rrna-based microarray with probes for over 300 bacterial species to better define the bacterial profiles of healthy root surfaces and root caries (rc) in the elderly. supragingival plaque was collected from 20 healthy subjects (controls) and from healthy and carious roots and carious dentin from 21 rc subjects (patients). collectively, 179 bacterial species and species groups were detected. a higher bacterial diversity was observed in controls than in patients. l ... | 2009 | 19039610 |
| detection and isolation of campylobacter species other than c. jejuni from children with crohn's disease. | the presence of campylobacter species other than campylobacter jejuni and antibodies to campylobacter concisus in children were investigated. a significantly greater presence of c. concisus and higher levels of antibodies to c. concisus were detected in children with crohn's disease (cd) than in controls. campylobacter species other than c. jejuni were isolated from intestinal biopsy specimens of children with cd. | 2009 | 19052183 |
| detection and isolation of campylobacter species other than c. jejuni from children with crohn's disease. | the presence of campylobacter species other than campylobacter jejuni and antibodies to campylobacter concisus in children were investigated. a significantly greater presence of c. concisus and higher levels of antibodies to c. concisus were detected in children with crohn's disease (cd) than in controls. campylobacter species other than c. jejuni were isolated from intestinal biopsy specimens of children with cd. | 2009 | 19052183 |
| the vaginal microflora in relation to gingivitis. | gingivitis has been linked to adverse pregnancy outcome (apo). bacterial vaginosis (bv) has been associated with apo. we assessed if bacterial counts in bv is associated with gingivitis suggesting a systemic infectious susceptibilty. | 2009 | 19161595 |
| is obesity an oral bacterial disease? | the world-wide explosion of overweight people has been called an epidemic. the inflammatory nature of obesity is widely recognized. could it really be an epidemic involving an infectious agent? in this climate of concern over the increasing prevalence of overweight conditions in our society, we focus on the possible role of oral bacteria as a potential direct contributor to obesity. to investigate this possibility, we measured salivary bacterial populations of overweight women. saliva was collec ... | 2009 | 19587155 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| characterization of slackia exigua isolated from human wound infections, including abscesses of intestinal origin. | eleven clinical strains isolated from infected wound specimens were subjected to polyphasic taxonomic analysis. sequence analysis of the 16s rrna gene showed that all 11 strains were phylogenetically related to slackia exigua. additionally, conventional and biochemical tests of 6 of the 11 strains were performed as supplementary methods to obtain phenotypic identification by comparison with the phenotypes of the relevant type strains. s. exigua has been considered an oral bacterial species in th ... | 2010 | 20107092 |
| transmission of diverse oral bacteria to murine placenta: evidence for the oral microbiome as a potential source of intrauterine infection. | microbial infection of the intrauterine environment is a major cause of preterm birth. the current paradigm indicates that intrauterine infections predominantly originate from the vaginal tract, with the organisms ascending into the sterile uterus. with the improvements in technology, an increasing number of bacterial species have been identified in intrauterine infections that do not belong to the vaginal microflora. we have demonstrated previously that intrauterine infections can originate fro ... | 2010 | 20123706 |
| the internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the campylobacter genus. | the campylobacter genus consists of a number of important human and animal pathogens. although the 16s rrna gene has been used extensively for detection and identification of campylobacter species, there is currently limited information on the 23s rrna gene and the internal transcribed spacer (its) region that lies between the 16s and 23s rrna genes. we examined the potential of the 23s rrna gene and the its region to be used in species differentiation and delineation of systematic relationships ... | 2010 | 20348308 |
| obstacles of multiplex real-time pcr for bacterial 16s rdna: primer specifity and dna decontamination of taq polymerase. | background: the detection of a broad range of bacteria by pcr is applied for the screening of blood and blood products with special attention to platelet concentrates. for practical use it is desirable that detection systems include gram-positive, gram-negative and non-gram-stainable bacteria. it is quite challenging to achieve high sensitivity along with a clear negative control with pcr reagents, because especially taq polymerase is contaminated with traces of bacterial dna. methods: bacterial ... | 2010 | 20737013 |
| design of 16s rrna gene primers for 454 pyrosequencing of the human foregut microbiome. | to design and validate broad-range 16s rrna primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome. | 2010 | 20806429 |
| bacterial 16s sequence analysis of severe caries in young permanent teeth. | previous studies have confirmed the association of the acid producers streptococcus mutans and lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. in addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in ph homeostasis. the bacterial community composition in health and in severe caries of the young permanent dentition ... | 2010 | 20826648 |
| clonal analysis of the microbiota of severe early childhood caries. | severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries. | 2010 | 20861633 |
| host attachment, invasion, and stimulation of proinflammatory cytokines by campylobacter concisus and other non-campylobacter jejuni campylobacter species. | campylobacter concisus and other non-campylobacter jejuni campylobacter species have been implicated in the initiation of gastrointestinal diseases. in the present study, we investigated the interaction between these bacteria and the human intestinal epithelium and immune cells. | 2010 | 21050118 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| three cases of severe invasive infections caused by campylobacter rectus and first report of fatal c. rectus infection. | we report the first fatal case of campylobacter rectus infection due to a subdural empyema and ruptured mycotic intracranial aneurysm and two cases of limb-threatening c. rectus necrotizing soft tissue and bone infection and empyema thoracis that responded to amoxicillin-clavulanate and surgical debridement and drainage. all three strains were identified by 16s rrna sequencing. | 2011 | 21270212 |
| a novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and dna viral systems. | the use of nucleases as toxins for defense, offense or addiction of selfish elements is widely encountered across all life forms. using sensitive sequence profile analysis methods, we characterize a novel superfamily (the sukh superfamily) that unites a diverse group of proteins including smi1/knr4, pgs2, fbxo3, skip16, syd, herpesviral us22, irs1 and trs1, and their bacterial homologs. using contextual analysis we present evidence that the bacterial members of this superfamily are potential imm ... | 2011 | 21306995 |
| rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms. | approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ... | 2011 | 21375703 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing. | the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed. | 2011 | 21625744 |
| detection of campylobacter concisus and other campylobacter species in colonic biopsies from adults with ulcerative colitis. | the critical role of bacteria in the pathogenesis of ulcerative colitis (uc) is well recognized, but an individual causative microorganism has not been singled out so far. campylobacter concisus and other non-jejuni species of campylobacter have been implicated as putative aetiological agents in inflammatory bowel disease in children, but such studies have not been addressed in adults. this study investigated the prevalence of campylobacter species in colonic biopsy samples from adults with uc a ... | 2011 | 21738679 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| Comparative Genomics of Helicobacter pylori and the human-derived Helicobacter bizzozeronii CIII-1 strain reveal the molecular basis of the zoonotic nature of non-pylori gastric Helicobacter infections in humans. | ABSTRACT: | 2011 | 22039924 |
| correlation network analysis applied to complex biofilm communities. | the complexity of the human microbiome makes it difficult to reveal organizational principles of the community and even more challenging to generate testable hypotheses. it has been suggested that in the gut microbiome species such as bacteroides thetaiotaomicron are keystone in maintaining the stability and functional adaptability of the microbial community. in this study, we investigate the interspecies associations in a complex microbial biofilm applying systems biology principles. using corr ... | 2011 | 22163302 |
| pathogenic potential of campylobacter ureolyticus. | the recent detection and isolation of the aflagellate campylobacter ureolyticus (previously known as bacteroides ureolyticus) from intestinal biopsy specimens and fecal samples of children with newly diagnosed crohn's disease led us to investigate the pathogenic potential of this bacterium. adherence and gentamicin protection assays were employed to quantify the levels of adherence to and invasion into host cells. c. ureolyticus unswcd was able to adhere to the caco-2 intestinal epithelial cell ... | 2011 | 22124656 |
| immunoreactive proteins of campylobacter concisus, an emergent intestinal pathogen. | campylobacter concisus is an emerging pathogen of the human gastrointestinal tract. recently, a significantly higher prevalence of c. concisus dna and higher levels of antibodies specific to c. concisus was detected in children with crohn's disease when compared with controls. the aim of this study was to identify c. concisus immunoreactive antigens. proteins from c. concisus were separated using two-dimensional gel electrophoresis, and sera from 10 c. concisus-positive children with crohn's dis ... | 2011 | 22092566 |
| campylobacter spp. as a foodborne pathogen: a review. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide. symptoms can range from mild to serious infections of the children and the elderly and permanent neurological symptoms. the organism is a cytochrome oxidase positive, microaerophilic, curved gram-negative rod exhibiting corkscrew motility and is carried in the intestine of many wild and domestic animals, particularly avian species including poultry. intestinal colonization results in healthy ... | 2011 | 21991264 |
| new approaches for isolation of previously uncultivated oral bacteria. | a significant number of microorganisms from the human oral cavity remain uncultivated. this is a major impediment to the study of human health since some of the uncultivated species may be involved in a variety of systemic diseases. we used a range of innovations previously developed to cultivate microorganisms from the human oral cavity, focusing on anaerobic species. these innovations include (i) in vivo cultivation to specifically enrich for species actively growing in the oral cavity (the "m ... | 2012 | 22057871 |
| prevalence of campylobacter species in adult crohn's disease and the preferential colonization sites of campylobacter species in the human intestine. | crohn's disease (cd) and ulcerative colitis (uc) are the two major forms of inflammatory bowel disease (ibd). a high prevalence of campylobacter concisus was previously detected in paediatric cd and adult uc. currently, the prevalence of c. concisus in adult cd and the preferential colonization sites of campylobacter species in the human intestine are unknown. in this study, we examined the prevalence of campylobacter species in biopsies collected from multiple anatomic sites of adult patients w ... | 2011 | 21966525 |
| comparative characterization of the virulence gene clusters (lipooligosaccharide [los] and capsular polysaccharide [cps]) for campylobacter coli, campylobacter jejuni subsp. jejuni and related campylobacter species. | campylobacter jejuni subsp. jejuni and campylobacter coli are leading causes of gastroenteritis, with virulence linked to cell surface carbohydrate diversity. although the associated gene clusters are well studied for c. jejuni subsp. jejuni, c. coli has been largely neglected. here we provide comparative analysis of the lipooligosaccharide (los) and capsular polysaccharide (cps) gene clusters, using genome and cluster sequence data for 36 c. coli strains, 67 c. jejuni subsp. jejuni strains and ... | 2012 | 23279811 |
| comparative characterization of the virulence gene clusters (lipooligosaccharide [los] and capsular polysaccharide [cps]) for campylobacter coli, campylobacter jejuni subsp. jejuni and related campylobacter species. | campylobacter jejuni subsp. jejuni and campylobacter coli are leading causes of gastroenteritis, with virulence linked to cell surface carbohydrate diversity. although the associated gene clusters are well studied for c. jejuni subsp. jejuni, c. coli has been largely neglected. here we provide comparative analysis of the lipooligosaccharide (los) and capsular polysaccharide (cps) gene clusters, using genome and cluster sequence data for 36 c. coli strains, 67 c. jejuni subsp. jejuni strains and ... | 2012 | 23279811 |
| bacterial diversity of subgingival plaque in 6 healthy chinese individuals. | the subgingival microbial ecology is complex, and little is known regarding its bacteria species composition in healthy chinese individuals. this study aimed to identify the subgingival microbiota from 6 healthy chinese subjects. subgingival samples from 6 volunteers were collected, the 16s rrna gene was amplified using broad-range bacterial primers, and clone libraries were constructed. for the initial 2,439 sequences analyzed, 383 species-level operational taxonomic units (slotus) belonging to ... | 2011 | 22977615 |
| design and evaluation of useful bacterium-specific pcr primers that amplify genes coding for bacterial 16s rrna. | we report the design and evaluation of pcr primers 63f and 1387r for amplification of 16s rrna genes from bacteria. their specificity and efficacy were tested systematically with a bacterial species and environmental samples. they were found to be more useful for 16s rrna gene amplification in ecological and systematic studies than pcr amplimers that are currently more generally used. | 1998 | 9464425 |
| relationships between subgingival microbiota and gcf biomarkers in generalized aggressive periodontitis. | to examine relationships between subgingival biofilm composition and levels of gingival crevicular fluid (gcf) cytokines in periodontal health and generalized aggressive periodontitis (gap). | 2010 | 20447254 |
| changes in the subgingival biofilm composition after coronally positioned flap. | this study evaluated the effects of coronally positioned flap (cpf) on the subgingival biofilm composition. | 2011 | 21437473 |
| metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study. | this study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. dna was extracted from subgingival samples and subjected to pcr amplification of the c2-c4 regions of the 16s rdna using primers with bar codes to identify individual samples. the pcr products were pooled and sequenced for the v4 region of the 16s rdna using the 454 flx standard platform. the results were analyzed f ... | 2012 | 23341849 |
| comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. | persistence of microorganisms or reinfections are the main reasons for failure of root canal therapy. very few studies to date have included culture-independent methods to assess the microbiota, including non-cultivable microorganisms. the aim of this study was to combine culture methods with culture-independent cloning methods to analyze the microbial flora of root-filled teeth with periradicular lesions. twenty-one samples from previously root-filled teeth were collected from patients with per ... | 2012 | 23152922 |
| microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization. | to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects. | 2012 | 22335194 |
| a consortium of aggregatibacter actinomycetemcomitans, streptococcus parasanguinis, and filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis. | aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (lap) in african-american adolescents has been documented but is poorly understood. two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of a. actinomycetemcomitans in their oral cavity. seventy-one a. actinomycetemcomitans-negative and 63 a. actinomycetemcomitans-positive periodontally healthy subjects were enrolled, sampled, examined, and radiographed ... | 2013 | 23784124 |
| bacterial community development in experimental gingivitis. | current knowledge of the microbial composition of dental plaque in early gingivitis is based largely on microscopy and cultural methods, which do not provide a comprehensive description of oral microbial communities. this study used 454-pyrosequencing of the v1-v3 region of 16s rrna genes (approximately 500 bp), and bacterial culture, to characterize the composition of plaque during the transition from periodontal health to gingivitis. a total of 20 healthy volunteers abstained from oral hygiene ... | 2013 | 23967169 |
| predictive, preventive, personalised and participatory periodontology: 'the 5ps age' has already started. | an impressive progress in dentistry has been recorded in the last decades. in order to reconsider guidelines in dentistry, it is required to introduce new concepts of personalised patient treatments: the wave of predictive, preventive and personalised medicine is rapidly incoming in dentistry. worldwide dentists have to make a big cultural effort in changing the actual 'reactive' therapeutic point of view, belonging to the last century, into a futuristic 'predictive' one. the first cause of toot ... | 2013 | 23763842 |
| lessons learned and unlearned in periodontal microbiology. | periodontal diseases are initiated by bacterial species living in polymicrobial biofilms at or below the gingival margin and progress largely as a result of the inflammation elicited by specific subgingival species. in the past few decades, efforts to understand the periodontal microbiota have led to an exponential increase in information about biofilms associated with periodontal health and disease. in fact, the oral microbiota is one of the best-characterized microbiomes that colonize the huma ... | 2013 | 23574465 |
| effect of periodontal therapy on the subgingival microbiota over a 2-year monitoring period. i. overall effect and kinetics of change. | to examine the 2-year post-therapy kinetics of change in the composition of subgingival biofilms. | 2013 | 23710672 |
| s-nitrosoglutathione accelerates recovery from 5-fluorouracil-induced oral mucositis. | mucositis induced by anti-neoplastic drugs is an important, dose-limiting and costly side-effect of cancer therapy. | 2014 | 25478918 |
| newly identified pathogens associated with periodontitis: a systematic review. | there is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. however, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. the aim of this systematic re ... | 2014 | 25074492 |
| pathogens and host immunity in the ancient human oral cavity. | calcified dental plaque (dental calculus) preserves for millennia and entraps biomolecules from all domains of life and viruses. we report the first, to our knowledge, high-resolution taxonomic and protein functional characterization of the ancient oral microbiome and demonstrate that the oral cavity has long served as a reservoir for bacteria implicated in both local and systemic disease. we characterize (i) the ancient oral microbiome in a diseased state, (ii) 40 opportunistic pathogens, (iii) ... | 2014 | 24562188 |
| the influence of smoking on the peri-implant microbiome. | smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ... | 2015 | 26124222 |
| a randomized trial of ethyl lauroyl arginate-containing mouthrinse in the control of gingivitis. | this 4-week, single-centre, randomized, examiner-blind, controlled study investigated the efficacy and safety of 0.15% ethyl lauroyl arginate (lae)-containing mouthrinse in adults with mild-to-moderate gingivitis. | 2015 | 26087864 |
| long-term evaluation of the antimicrobial susceptibility and microbial profile of subgingival biofilms in individuals with aggressive periodontitis. | this study evaluates the antimicrobial susceptibility and composition of subgingival biofilms in generalized aggressive periodontitis (gap) patients treated using mechanical/antimicrobial therapies, including chlorhexidine (chx), amoxicillin (amx) and metronidazole (met). gap patients allocated to the placebo (c, n = 15) or test group (t, n = 16) received full-mouth disinfection with chx, scaling and root planning, and systemic amx (500 mg)/met (250 mg) or placebos. subgingival plaque samples we ... | 2015 | 26273264 |
| functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. | periodontitis is a polymicrobial biofilm-induced inflammatory disease that affects 743 million people worldwide. the current model to explain periodontitis progression proposes that changes in the relative abundance of members of the oral microbiome lead to dysbiosis in the host-microbiome crosstalk and then to inflammation and bone loss. using combined metagenome/metatranscriptome analysis of the subgingival microbiome in progressing and non-progressing sites, we have characterized the distinct ... | 2015 | 25918553 |
| prelude to oral microbes and chronic diseases: past, present and future. | associations between oral and systemic health are ancient. oral opportunistic bacteria, particularly, porphyromonas gingivalis and fusobacterium nucleatum, have recently been deviated from their traditional roles as periodontal pathogens and arguably ascended to central players based on their participations in complex co-dependent mechanisms of diverse systemic chronic diseases risk and pathogenesis, including cancers, rheumatoid-arthritis, and diabetes. | 2015 | 25813714 |
| relationship between invasion of the periodontium by periodontal pathogens and periodontal disease: a systematic review. | bacterial invasion of the periodontal tissues has been suggested as a relevant step in the etiopathogenesis of periodontal disease. however, its exact importance remains to be defined. the present systematic review assessed the scientific evidence concerning the relationship between the quality or quantity of periodontal microbiota in periodontal tissues and development of periodontal disease. the databases medline-pubmed, cochrane-central, isi web of knowledge and scopus were searched, up to ja ... | 2015 | 25654367 |
| microbial profiles and detection techniques in peri-implant diseases: a systematic review. | to describe the microbial profiles of peri-implant diseases and the main detection methods. | 2016 | 27833735 |
| accuracy of commercial kits and published primer pairs for the detection of periodontopathogens. | despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens. | 2016 | 27020914 |
| periodontal disease's contribution to alzheimer's disease progression in down syndrome. | people with down syndrome (ds) are at an increased risk for alzheimer's disease (ad). after 60 years of age, >50% of ds subjects acquire dementia. nevertheless, the age of onset is highly variable possibly because of both genetic and environmental factors. genetics cannot be modified, but environmental risk factors present a potentially relevant intervention for ds persons at risk for ad. among them, inflammation, important in ad of ds type, is potential target. consistent with this hypothesis, ... | 2016 | 27239536 |
| a persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations. | acute exacerbations of chronic obstructive pulmonary disease (copd) are a major source of morbidity and contribute significantly to healthcare costs. although bacterial infections are implicated in nearly 50% of exacerbations, only a handful of pathogens have been consistently identified in copd airways, primarily by culture-based methods, and the bacterial microbiota in acute exacerbations remains largely uncharacterized. the aim of this study was to comprehensively profile airway bacterial com ... | 2010 | 20141328 |
| xer recombinase and genome integrity in helicobacter pylori, a pathogen without topoisomerase iv. | in the model organism e. coli, recombination mediated by the related xerc and xerd recombinases complexed with the ftsk translocase at specialized dif sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation at cell division. computational genome analysis of helicobacter pylori, a slow growing gastric pathogen, identified just one chromosomal xer gene (xerh) and its cognate dif site (difh). here we show that recombination between directly repeated difh sit ... | 2012 | 22511919 |
| genome sequence of campylobacter showae unswcd, isolated from a patient with crohn's disease. | campylobacter showae unswcd was isolated from a patient with crohn's disease. here we present a 2.1 mb draft assembly of its genome. | 2013 | 23409253 |
| genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus campylobacter. | the genus campylobacter includes some of the most relevant pathogens for human and animal health; the continuous effort in their characterization has also revealed new species putatively involved in different kind of infections. nowadays, the available genomic data for the genus comprise a wide variety of species with different pathogenic potential and niche preferences. in this work, we contribute to enlarge this available information presenting the first genome for the species campylobacter sp ... | 2014 | 25193310 |
| biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ... | 2014 | 24498018 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| multicenter evaluation of clinical diagnostic methods for detection and isolation of campylobacter spp. from stool. | the use of culture-independent diagnostic tests (cidts), such as stool antigen tests, as standalone tests for the detection of campylobacter in stool is increasing. we conducted a prospective, multicenter study to evaluate the performance of stool antigen cidts compared to culture and pcr for campylobacter detection. between july and october 2010, we tested 2,767 stool specimens from patients with gastrointestinal illness with the following methods: four types of campylobacter selective media, f ... | 2016 | 26962088 |
| comprehensive genomic characterization of campylobacter genus reveals some underlying mechanisms for its genomic diversification. | campylobacter species.are phenotypically diverse in many aspects including host habitats and pathogenicities, which demands comprehensive characterization of the entire campylobacter genus to study their underlying genetic diversification. up to now, 34 campylobacter strains have been sequenced and published in public databases, providing good opportunity to systemically analyze their genomic diversities. in this study, we first conducted genomic characterization, which includes genome-wide alig ... | 2013 | 23940551 |
| bacterial protein n-glycosylation: new perspectives and applications. | protein glycosylation is widespread throughout all three domains of life. bacterial protein n-glycosylation and its application to engineering recombinant glycoproteins continue to be actively studied. here, we focus on advances made in the last 2 years, including the characterization of novel bacterial n-glycosylation pathways, examination of pathway enzymes and evolution, biological roles of protein modification in the native host, and exploitation of the n-glycosylation pathways to create nov ... | 2013 | 23329827 |
| global epidemiology of campylobacter infection. | campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. the incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. the dramatic increase in north america, europe, and australia is alarming, and data from parts of africa, asia, and the middle east indicate that campylobacteriosis is endemic in these areas, especially in children. in addition to c. jejuni, there is increasing rec ... | 2015 | 26062576 |
| phylogenetic identification of bacterial mazf toxin protein motifs among probiotic strains and foodborne pathogens and potential implications of engineered probiotic intervention in food. | | 2012 | 23186337 |
| co-occurrence of anaerobic bacteria in colorectal carcinomas. | numerous cancers have been linked to microorganisms. given that colorectal cancer is a leading cause of cancer deaths and the colon is continuously exposed to a high diversity of microbes, the relationship between gut mucosal microbiome and colorectal cancer needs to be explored. metagenomic studies have shown an association between fusobacterium species and colorectal carcinoma. here, we have extended these studies with deeper sequencing of a much larger number (n = 130) of colorectal carcinoma ... | 2013 | 24450771 |
| the microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the biscuit study. | children presenting for the first time with inflammatory bowel disease (ibd) offer a unique opportunity to study aetiological agents before the confounders of treatment. microaerophilic bacteria can exploit the ecological niche of the intestinal epithelium; helicobacter and campylobacter are previously implicated in ibd pathogenesis. we set out to study these and other microaerophilic bacteria in de-novo paediatric ibd. | 2013 | 23554935 |
| role of the gut microbiota in inflammatory bowel disease pathogenesis: what have we learnt in the past 10 years? | our understanding of the microbial involvement in inflammatory bowel disease (ibd) pathogenesis has increased exponentially over the past decade. the development of newer molecular tools for the global assessment of the gut microbiome and the identification of nucleotide-binding oligomerization domain-containing protein 2 in 2001 and other susceptibility genes for crohn's disease in particular has led to better understanding of the aetiopathogenesis of ibd. the microbial studies have elaborated ... | 2014 | 24574795 |
| oral campylobacter species: initiators of a subgroup of inflammatory bowel disease? | in recent years, a number of studies detected a significantly higher prevalence of campylobacter species such as campylobacter concisus (c. concisus) in intestinal biopsies and fecal samples collected from patients with inflammatory bowel disease (ibd) compared to controls. most of these campylobacter species are not of zoonotic origin but are human oral campylobacter species. bacterial species usually cause diseases in the location where they colonize. however, c. concisus and other oral campyl ... | 2015 | 26309350 |