| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| analysis of cell surface antigens of campylobacter rectus. | campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ... | 1992 | 1303324 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis. | the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ... | 1992 | 1325865 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| pore-forming ability of major outer membrane proteins from wolinella recta atcc 33238. | three major outer membrane proteins with apparent molecular masses of 43, 45, and 51 kda were purified from wolinella recta atcc 33238, and their pore-forming abilities were determined by the black lipid bilayer method. the non-heat-modifiable 45-kda protein (omp 45) showed no pore-forming activity even at high kcl concentrations. the single-channel conductances in 1 m kcl of the heat-modifiable proteins with apparent molecular masses of 43 kda (omp 43) and 51 kda (omp 51) were 0.49 and 0.60 ns, ... | 1992 | 1370429 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| wolinella recta in adult gingivitis and periodontitis. | wolinella recta has been associated with adult periodontitis, but its role in the disease remains uncertain. this report clarifies the distribution of w. recta in periodontally healthy and diseased subjects, and treated patients with recurrent disease. a specific polyclonal rabbit antiserum against w. recta strain 372 was used for indirect immunofluorescence localization of w. recta in dental plaque from untreated and treated patients. supragingival plaque was collected from 15 periodontally hea ... | 1992 | 1531512 |
| production of an extracellular toxin by the oral pathogen campylobacter rectus. | the atcc type strain and six clinical isolates of campylobacter rectus were tested for toxicity against hl-60 cells and human polymorphonuclear neutrophils (pmns). after challenge with bacterial cell suspensions and media supernatants for up to 4 h, eukaryotic cell viability was assayed by trypan blue dye exclusion and lactate dehydrogenase release. cells of the c. rectus type strain were not toxic. however, ethanol and (nh4)2so4 extracts of culture media supernatants killed hl-60 cells in a tim ... | 1992 | 1560755 |
| the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid. | in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ... | 1990 | 1696949 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
| the microflora of periodontal sites showing active destructive progression. | 20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ... | 1991 | 1752997 |
| antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens. | nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ... | 1991 | 1812907 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes. | although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ... | 1991 | 1854636 |
| virulence of wolinella recta in a murine abscess model. | the virulence of wolinella recta isolates was studied in an experimental animal model by using monoinfection of balb/c mice. infection with clinical isolates of w. recta 576 and w. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas w. recta atcc 33238 failed to induce a similar lesion. histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum pr ... | 1991 | 1855996 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| extraction, purification, and characterization of major outer membrane proteins from wolinella recta atcc 33238. | the outer membrane of wolinella recta atcc 33238 was isolated by french pressure cell disruption and differential centrifugation. outer membrane proteins (omps) were solubilized by zwittergent 3.14 extraction and separated by deae-sephacel ion-exchange chromatography. the major omps that were found in w. recta atcc 33238 and in several other wolinella spp. consisted of proteins with apparent molecular masses of 51, 45, and 43 kda. these three conserved proteins were purified to essential homogen ... | 1991 | 1894372 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| comparative study of lipopolysaccharides from wolinella recta, w. curva, w. succinogenes and campylobacter sputorum ssp. sputorum. | lipopolysaccharides (lps) were extracted from cells of wolinella recta atcc 33238, w. curva atcc 33224, w. succinogenes atcc 29543 and campylobacter sputorum ssp. sputorum a 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. chemical compositions of the purified lps including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. all lps preparations contained a ... | 1991 | 1916228 |
| monoclonal antibody to a specific antigen from wolinella recta atcc 33238. | four monoclonal antibodies (m-abs) to a specific antigen (150-kda protein antigen) isolated from wolinella recta atcc 33238 by acid extraction were obtained. the four antibodies were all of the igg1 subclass and exhibited equally high specificity for the antigen. a battery of 14 strains or oral bacteria were screened for cross-reactivity with each mab by elisa and western blot analysis. weak cross-reactivity to some strains was observed which differed depending upon the mab. immuno-electromicros ... | 1991 | 1936935 |
| bacteroides ureolyticus (ntu) medium for the selective recovery of bacteroides gracilis. | bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. in this study, bacteroides ureolyticus (ntu) medium was tested for its ability to allow the growth of b. gracilis and other formate-fumarate requiring gram-negative anaerobes and to enable the recovery of these organisms from clinical specimens. all reference strains grew on ntu medium with the except ... | 1991 | 1942000 |
| analysis of in vitro lymphoproliferative responses and antibody formation following subcutaneous injection of actinobacillus actinomycetemcomitans and wolinella recta in a murine model. | the potential of wolinella recta and actinobacillus actinomycetemcomitans to cause abscesses and induce an immune response was tested in balb/c mice. mice were injected subcutaneously with w. recta, a. actinomycetemcomitans or a mixture of these 2 microorganisms. mice injected with a. actinomycetemcomitans alone, or with both organisms, demonstrated abscesses at the injection site 2 days later, from which pure cultures of a. actinomycetemcomitans were isolated. mice injected with w. recta had sm ... | 1991 | 1945478 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| aspartate and asparagine as electron acceptors for wolinella recta. | since fumarate and nitrate are not usually available in the oral ecosystem, it was investigated whether aspartate and asparagine could be used as alternative electron acceptors by wolinella recta, which is strictly dependent on a respiratory metabolism with formate or h2 as electron donors. both aspartate and asparagine were indeed shown to support growth of w. recta with formate as electron donor. fermentative growth with aspartate alone was not possible. succinate was the major end-product and ... | 1991 | 1945491 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| three-dimensional structure of the surface layer of wolinella recta. | the three-dimensional structure of the crystalline surface layer (s-layer) of wolinella recta atcc 33238t, a gram-negative, anaerobic periodontopathogen, was determined to 3.8 nm resolution by electron microscopy and digital image processing. the s-layer protein is closely associated with the outer bacterial membrane, and shows p6 symmetry with lattice spacing and thickness of 21 nm and 15 nm, respectively. the funnel-shaped subunits consist of 6 heavy domains located round a common base at the ... | 1990 | 2080071 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| monoclonal antibodies for the detection of 'periodontopathic' bacteria. | the development of indirect immunofluorescence assays using mabs as specific probes has made it possible to detect a variety of suspected periodontal pathogens in subgingival plaque, with exquisite sensitivity. the studies demonstrate high reproducibility of the results if different mabs are used to assess the same bacterial species. bacteroides forsythus, bact. gingivalis, bact. intermedius and wolinella recta, but not actinobacillus actinomycetemcomitans were found in sites of adult periodonti ... | 1990 | 2088218 |
| hydrophobicities of human polymorphonuclear leukocytes and oral bacteroides and porphyromonas spp., wolinella recta, and eubacterium yurii with special reference to bacterial surface structures. | the hydrophobicities of human polymorphonuclear leukocytes (pmnls) and bacteroides buccae, b. oris, b. oralis, b. veroralis, b. buccalis, b. heparinolyticus, b. intermedius, b. denticola, b. loescheii, b. melaninogenicus, porphyromonas gingivalis, p. endodontalis, wolinella recta, and eubacterium yurii were studied by the hexadecane method. the majority of the strains were equally or less hydrophobic than the pmnls. only in the case of e. yurii and the only strain of b. buccalis were all strains ... | 1990 | 2091243 |
| effect of actinobacillus actinomycetemcomitans, wolinella recta and bacteroides gingivalis on the viability of retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | we studied the interactions between viable and heat-killed, opsonized and unopsonized periodontopathic bacteria with both uninduced and induced hl-60 promyelocytic leukemia cells. the cells were induced to differentiate into granulocyte-like cells by incubation with retinoic acid (ra) or dimethyl sulfoxide (dmso). when unopsonized, wolinella recta atcc 33228 significantly suppressed the net proliferation of uninduced hl-60 cells, actinobacillus actinomycetemcomitans strain y4 was markedly lethal ... | 1990 | 2098697 |
| interaction of gram-negative periodontal pathogens with retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | as a first step toward elucidating the reasons for differences among periodontal pathogens in their cytotoxic effects on hl-60 cells, we used transmission electron microscopy to examine morphological aspects of granulocyte-bacteria interactions. unopsonized actinobacillus actinomycetemcomitans strain y4 and bacteroides gingivalis atcc 33277 adhered to, and were phagocytosed by, retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. in contrast, there was only minimal interaction betwe ... | 1990 | 2098698 |
| sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. | a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ... | 1990 | 2108991 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations. | a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ... | 1990 | 2202809 |
| plaque and chronic inflammatory periodontal disease. a question of ecology. | the nature of the relationship between dental plaque and chronic inflammatory periodontal disease (cipd) remains unclear, although there is no doubt that plaque is the direct cause. non-specific, specific and exogenous hypotheses have been proposed to explain plaque-host relationships. current evidence indicates that plaque is part of the natural human microflora, one of many such in nature, and that disruption of oral microbial ecology, due primarily to diet texture changes, leads to gingivitis ... | 1990 | 2212083 |
| associations between six dna probe-detected periodontal bacteria and alveolar bone loss and other clinical signs of periodontitis. | the purpose of the present study was to assess the associations between the presence and amounts of actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius, eikenella corrodens, wolinella recta, and fusobacterium nucleatum in the periodontal pocket and the degree of alveolar bone loss and other clinical signs of periodonitis, such as probing pocket depth, attachment level, and presence of bleeding on probing at the same site. the study material comprised 16 subjects with or ... | 1990 | 2288212 |
| species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. | oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ... | 1990 | 2312676 |
| surface characteristics of wolinella recta atcc 33238 and human clinical isolates: correlation of structure with function. | selected characteristics of the surface of wolinella recta atcc 33238 and three w. recta clinical isolates (ci) were studied as well as the adherence of these strains to human gingival fibroblasts (hgf). w. recta atcc 33238 and the ci were examined by electron microscopy, electrophoresis, isoelectric focusing, and adherence to hgf. electron microscopic examination of ci revealed the presence of a periodic paracrystalline layer external to and associated with the outer membrane. this surface laye ... | 1990 | 2387623 |
| antimicrobial effect of chlorhexidine in a controlled release delivery system. | chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ... | 1990 | 2390965 |
| ultrastructural relationship of cell envelope layers in wolinella recta. | the cell envelope of wolinella recta was studied by electron microscopy. in thin sections the surface layer (s-layer) seemed to be in close contact to the outer membrane (om). a hexagonally arranged pattern of the s-layer was revealed by freeze-etching. unlike in other oral bacteria with an s-layer, the cleavage occurred along the s-layer by freeze-fracture, suggesting an exceptionally tight relationship between the two outermost layers. the convex surface of the s-layer was usually revealed by ... | 1989 | 2469124 |
| dna-dna hybridization incompatibility of campylobacter pylori with other campylobacter and wolinella species. | dna-dna hybridization in solution was used to characterize 23 human isolates of campylobacter pylori. the 23 isolates showed dna affinity with the type strain (nctc 11637). the relative binding ratios varied between 0.83 and 1. type strains of c. coli (nctc 11366), c. jejuni (nctc 11351), c. laridis (nctc 11352), c. sputorum subsp. sputorum (atcc 35980), wolinella recta (nctc 11489) and w. succinogenes (atcc 29543) showed relative binding ratios less than 0.01 compared to the c. pylori type stra ... | 1989 | 2499346 |
| chemical and biological properties of lipopolysaccharide, lipid a and degraded polysaccharide from wolinella recta atcc 33238. | lipopolysaccharide (lps) was isolated and purified from wolinella recta atcc 33238 by the phenol-water procedure and rnaase treatment. the sugar components of the lps were rhamnose, mannose, glucose, heptose, 2-keto-3-deoxyoctonate (kdo) (3-deoxy-d-manno-octulosonate) and glucosamine. the degraded polysaccharide prepared from lps by mild acid hydrolysis was fractionated by sephadex g-50 gel chromatography into three fractions: (1) a high-molecular-mass fraction, eluting just behind the void volu ... | 1989 | 2600585 |
| newly delineated periodontal pathogens with special reference to selenomonas species. | many new species have been isolated from subgingival periodontal pockets, for example wolinella recta and bacteroides forsythus, reflecting better basic microbiological techniques. other species were created from existing species as a result of better characterization methods, i.e. bacteroides buccae and bacteroides oris. we can recognize different types of periodontal disease and can find differences relating to the progressive compared to inactive lesions. data illustrated in this presentation ... | 1989 | 2661440 |
| wolinella recta and spirochetes in relation to alteration of probing depth in a population without regular dental care. | the purpose of this study was to determine the occurence of spirochetes and wolinella sp. in untreated, nonselected subjects and to relate the presence of these organisms to changes in probing depth over a period of 180 days. clinical parameters were recorded and subgingival microbial samples were taken mesiobuccally on the upper first molars of 120 subjects (mean age 37 +/- 14 years) with a history of no regular dental care. the number of motile rods and spirochetes were determined in the darkf ... | 1989 | 2678458 |
| [microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy]. | the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ... | 1989 | 2700361 |
| subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance. | hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ... | 1989 | 2704977 |
| campylobacter-wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium. | most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. arylsulfatase activity was, however, restricted to campylobacter-wolinella group organisms, including wolinella recta, a possible periodontopathogen. w. recta was the only arylsulfatase-active species against which consistently high levels of antibody were detected in human sera. | 1989 | 2707850 |
| a comparison of bacteroides ureolyticus isolates from different clinical sources. | clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as bacteroides ureolyticus) from superficial ulcers and soft tissue infections (15), non-gonococcal, non-chlamydial urethritis (12) and adult periodontal disease (14) were compared with reference strains of b. ureolyticus, b. gracilis and wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatogr ... | 1989 | 2724326 |
| cell surface protein antigen from wolinella recta atcc 33238t. | a high-molecular-weight (approximately 150,000) protein was selectively isolated by acid extraction from the cell surface of wolinella recta and purified by negative adsorption on deae-cellulose and gel filtration. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in w. recta but not in other wolinella species, such as w. curva and w. succinogenes. sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera fr ... | 1989 | 2753998 |
| the microbiota of early periodontitis lesions in adults. | the predominant cultivable microbiota of shallow progressive (active) periodontal lesions in 4 adults (29-40 years) was compared to that of shallow inactive sites. periodontal attachment levels were monitored longitudinally, and sites showing 2-3 mm attachment loss in the previous 1-2 months were considered disease active. inactive sites showed no change in attachment level. the microbiota of shallow sites was dominated by gram negative rod species including wolinella recta, bacteroides forsythu ... | 1989 | 2768541 |
| nutritional relationships between oral bacteria. | nutritional relationships were revealed during the coculturing of bacteroides gingivalis with wolinella recta and bacteroides melaninogenicus with w. recta. w. recta produced a substance that stimulated the growth of b. gingivalis and b. melaninogenicus. characterization by thin-layer chromatography and absorption spectrometry identified the compound as protoheme. production of large amounts of formate by b. melaninogenicus stimulated the growth of w. recta. these nutritional relationships could ... | 1986 | 2875029 |
| wolinella recta, campylobacter concisus, bacteroides gracilis, and eikenella corrodens from periodontal lesions. | | 1987 | 2957486 |
| lipopolysaccharide-stimulated pge2 release from human monocytes. comparison of lipopolysaccharides prepared from suspected periodontal pathogens. | lipopolysaccharides (lps) prepared from the suspected periodontal pathogens actinobacillus actinomycetemcomitans (a. a.), bacteroides gingivalis, b. intermedius and wolinella recta were compared to salmonella typhimurium lps for their capacity to stimulate prostaglandin e2 (pge2) release from human monocytes. counterflow isolated monocytes were cultured with control medium or media containing 10 micrograms/ml lps. media were then exchanged every 24 hours for a total of 72 hours. salmonella and w ... | 1988 | 3054046 |
| production and characterization of monoclonal antibodies against bacteroides forsythus and wolinella recta. | hybrid cell lines producing monoclonal antibodies against bacteroides forsythus or wolinella recta were generated by fusion of sp2/0 or fo myeloma cells with splenocytes from balb/c mice immunized with formalinized cells of b. forsythus fdc 331 or w. recta d13a-g, respectively. enzyme-linked immunosorbent assays and indirect immunofluorescence tests were used to analyze the distribution of the recognized antigens on a panel of 70 strains representing 35 taxa, most of which are members of the ora ... | 1988 | 3170894 |
| the comparative cytotoxicity of periodontal bacteria. | the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ... | 1988 | 3204481 |
| chemical and biological characterization of the lipopolysaccharide of the oral pathogen wolinella recta atcc 33238. | to investigate the potential pathogenic mechanisms of the oral periodontopathogen wolinella recta atcc 33238, we have isolated its lipopolysaccharide (lps) and determined the chemical composition and selected in vitro biological activities of the molecule. sodium desoxycholate-polyacrylamide gel electrophoresis revealed the w. recta lps to be an atypical smooth lps with short o-antigenic side chains. chemically the lps consisted of 47.2% lipid a, 19.6% polysaccharide, 9.0% heptose, 8.5% hexosami ... | 1988 | 3260893 |
| microbiological and immunological studies of adult periodontitis in patients with noninsulin-dependent diabetes mellitus. | the subgingival microflora and serum antibody response was examined in periodontitis patients with noninsulin-dependent diabetes mellitus (niddm), impaired glucose tolerance (igt), and normal glucose tolerance (ngt). the predominant cultivable microflora was determined for subgingival plaque sampled from two deep periodontal pockets in each of eight adult periodontitis patients with niddm. indirect immunofluorescence for bacteroides intermedius, bacteroides gingivalis, and haemophilus actinomyce ... | 1988 | 3276868 |
| growth studies of wolinella recta, a gram-negative periodontopathogen. | | 1987 | 3507619 |
| chemotactic response to formate by campylobacter concisus and its potential role in gingival colonization. | cells of campylobacter concisus 288 were chemotactic toward formate, but not to any other compound tested. chemicals that were not chemoattractants included 20 sugars, inorganic salts, amino acids, and their derivatives, purines and pyrimidines, fatty acids, and natural mixtures such as saliva, serum, crevicular fluid, and mucin. chemotaxis was measured quantitatively by a modification of the capillary method. cells were suspended in 0.01 m tris buffer, ph 7.5, supplemented with 5 mm kcl, 0.1% n ... | 1986 | 3699887 |
| bacteroides buccae and related taxa in necrotic root canal infections. | fifty-seven adults with apical periodontitis were examined for the presence of nonpigmented bacteroides species in 62 infected root canals. nonpigmented bacteroides species were found in 35 canals. in four cases two nonpigmented bacteroides species and in one case three nonpigmented bacteroides species were found. species belonging to the b. fragilis group were not isolated. the most frequently isolated species were b. buccae (15 strains), b. oris (12 strains), and b. oralis (7 strains). alpha-f ... | 1986 | 3782459 |
| gram negative species associated with active destructive periodontal lesions. | apical subgingival plaque samples were taken from 19 subjects exhibiting active destructive periodontal disease. the predominant cultivable gram negative species from 50 active sites were compared to 69 inactive sites of comparable pocket depth and attachment level loss. active disease sites were chosen which showed a significant loss of attachment within a two-month interval. proportions of gram negative rods were higher in active periodontal disease sites than in inactive sites. species which ... | 1985 | 3863838 |
| the relationships between streptococcal species and periodontopathic bacteria in human dental plaque. | the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ... | 1985 | 3868968 |
| api zym and api an-ident reactions of fastidious oral gram-negative species. | api zym and api an-ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "bacteroides forsythus, the "fusiform" bacteroides species (a. c. r. tanner, m. a. listgarten, m. n. strzempko, and j. l. ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. the tests were able to separate this new species from species of capnocytophaga and fusobacterium ... | 1985 | 3930558 |
| comparison of strains of gram-negative, anaerobic, agar-corroding rods isolated from soft tissue infections in cats and dogs with type strains of bacteroides gracilis, wolinella recta, wolinella succinogenes, and campylobacter concisus. | a total of 64 strains of gram-negative, asaccharolytic, anaerobic, agar-corroding, rod-shaped bacteria from soft-tissue infections of cats and dogs were compared with other agar-corroding, anaerobic organisms isolated from human periodontal pockets (wolinella recta atcc 33238t), bovine rumens (wolinella succinogenes atcc 29543t), and gingival crevices of humans (bacteroides gracilis atcc 33236t and bacteroides ureolyticus nctc 10941t). campylobacter concisus atcc 33237t (from human gingival crev ... | 1984 | 6490858 |
| use of isoelectric focusing of bacterial proteins for characterisation of strains of motile anaerobic "corroding" bacteria isolated from soft tissue infections in cats and dogs. | isoelectric focusing of bacterial proteins in 1 mm thick agarose gels and ultrastructural analysis of cell walls were used to facilitate characterisation of motile "corroding" bacteria from subcutaneous abscesses and pyothorax infections from cats and dogs. some of the motile "corroding" rods had ultrastructural cell wall features identical with wolinella recta described from human periodontal pockets. other strains had the more conventional cell wall ultrastructure of gram negative bacteria. is ... | 1984 | 6499406 |
| isolation of wolinella recta and actinomyces viscosus from an actinomycotic chest wall mass. | in this note we describe a patient with an actinomycotic chest wall mass from which large numbers of wolinella recta and actinomyces viscosus organisms were isolated. this is the first reported association of w. recta with extraoral infection and the tenth report of lung infection by a. viscosus. | 1984 | 6520225 |
| osteomyelitis in dogs and cats caused by anaerobic bacteria. | localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ... | 1984 | 6732670 |
| similarity of wolinella recta strains isolated from periodontal pockets and root canals. | | 1982 | 6955433 |
| enzymatic characterization of some oral and nonoral gram-negative bacteria with the api zym system. | the api zym system (analytab products, plainview, n.y.), containing 19 chromogenic substrates, was utilized semiquantitatively to detect extracellular acid and alkaline phosphatases, aminopeptidases, proteases, esterase-lipase, phosphoamidase, and glycosidases in 128 oral and nonoral isolates of black-pigmented bacteroides, actinobacillus, haemophilus aphrophilus, capnocytophaga, fusobacterium nucleatum, wolinella recta, and veillonella parvula. in the black-pigmented bacteroides group of organi ... | 1981 | 7026598 |
| evaluation of periodontal treatments using controlled-release tetracycline fibers: microbiological response. | in a 12-month multi-center study of 116 adult periodontitis subjects, six putative periodontal pathogens were monitored by dna probe methods in a subset of 31 subjects. monitored species included porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn), eikenella corrodens (ec), campylobacter rectus (cr), and actinobacillus actinomycetemcomitans (aa) with an average detection limit of 1.8 x 10(4) bacterial colony forming units/sample. the microbiological response t ... | 1995 | 7473013 |
| energetics of molecular hydrogen oxidation in the oral pathogen campylobacter rectus. | | 1995 | 7548544 |
| detection of putative periodontal pathogens in subgingival specimens by 16s ribosomal dna amplification with the polymerase chain reaction. | the utility of the 16s ribosomal rna-based polymerase chain reaction (pcr) for detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia, and treponema denticola was examined and compared with that of anaerobic culture. primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16s ribosomal rna genes of these organisms. this method had a lower detection limit of 50 ta ... | 1995 | 7548580 |
| some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics. | the subgingival microflora and serum antibody response were examined in long-duration insulin-dependent diabetics and age- and sex-matched non-diabetics. the material consisted of 9 diabetics aged 40-49 years and 19 aged 50-59 years, 13 non-diabetics aged 40-49 years and 21 aged 50-59 years. the bacterial species studied (actinobacillus actinomycetemcomitans, campylobacter rectus, capnocytophaga spp, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia) w ... | 1995 | 7560223 |
| occurrence of certain bacterial species and morphotypes in juvenile periodontitis in chile. | the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, campylobacter rectus, capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (ljp) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (gjp) patients. the subgingival proportion of the 7 bacterial species was det ... | 1995 | 7562347 |
| periodontal microbiota of mobile and non-mobile teeth. | the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ... | 1995 | 7623258 |
| clinical and laboratory studies on a patient with early onset periodontitis and her family members. a case report. | extensive clinical, microbiological, hematological, and immunological studies were performed on a patient with early onset periodontitis (eop) and two other members of the family. the proband, a 27-year-old female, had early onset periodontitis and a high level of serum rheumatoid factors (rf) with no diagnosable medical disease. her mother had lost all her teeth at the age of 50 because of advanced periodontitis, while her elder sister was unaffected by periodontitis. neither the proband's peri ... | 1995 | 7623261 |
| campylobacter showae sp. nov., isolated from the human oral cavity. | nine campylobacter-like strains were isolated from human gingival crevices and characterized. these strains were gram-negative, straight rods that were motile by means of multiple unipolar flagella. they were asaccharolytic and preferred an anaerobic atmosphere rather than a microaerophilic atmosphere for growth, and their growth was stimulated by formate and fumarate. these strains were biochemically similar to campylobacter curvus and campylobacter rectus, but were clearly distinguishable from ... | 1993 | 7694633 |
| periodontal findings in spouses. a clinical, radiographic and microbiological study. | clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ... | 1995 | 7775676 |
| effect of campylobacter rectus lps on plasminogen activator-plasmin system in human gingival fibroblast cells. | the plasminogen activator (pa)-plasmin system is implicated in the degradation of the extracellular matrix in inflammation through activation of metalloproteases and prekallikrein. we examined the activation of the pa-plasmin system in human gingival fibroblast cells (gin-1 cells) following treatment with lipopolysaccharide (lps) from campylobacter rectus, which is frequently detected at sites of periodontal disease. the c. rectus lps stimulated the plasmin activity in the conditioned medium of ... | 1995 | 7776154 |
| periodontopathogens in elderly chinese with different periodontal disease experience. | if an etiological relationship exists between destructive periodontal disease and putative periodontopathogens, they would be expected to have a very low prevalence in periodontally healthy elderly persons. to test this hypothesis, 2 subgroups of elderly, rural chinese (a periodontally "best" and a "worst" group, each comprising 15 persons) were identified in 1990 from a cohort aged 55-69 years, examined in 1984. assessment of changes in periodontal status over the 6-year period were possible by ... | 1995 | 7790524 |
| effect of treatment on some periodontopathogens and their antibody levels in periodontal abscesses. | twenty patients suffering from abscess of periodontal origin were treated and followed clinically and microbiologically for 6 months. microbiological examination was performed by culture technique. one deep and one shallow periodontal pocket in the same patient were examined concomitantly. serum was collected and analyzed for specific antibody level using elisa methodology. treatment included supragingival scaling, drainage, and irrigation of the periodontal pocket with 0.85% sodium chloride and ... | 1994 | 7853125 |
| the gingival immune response to periodontal pathogens in juvenile periodontitis. | a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ... | 1994 | 7870467 |
| macaca fascicularis as a model in which to assess the safety and efficacy of a vaccine for periodontitis. | we have assessed macaca fascicularis as a potential model in which to test the efficacy and safety of a vaccine for periodontitis. twenty-eight animals were surveyed and 20 studied in more detail. clinical periodontal status was assessed, the subgingival microflora analyzed especially for the presence and proportions of porphyromonas gingivalis and titers and avidities of serum antibodies reactive with p. gingivalis measured. probing depths ranged from 0.90 mm to 3.80 mm, gingival index scores f ... | 1994 | 8008428 |
| interdental supragingival plaque--a natural habitat of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, and prevotella nigrescens. | the aim of this study was to test the hypothesis that suspected periodontal pathogens form a minor component of the supragingival plaque of individuals without periodontal diseases. twenty-one dental hygienist trainees with a mean age of 23.5 years were twice sampled for interdental plaque between 1st and 2nd molars in all quadrants. the samples were assessed for actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and the prevotella interm ... | 1994 | 8083438 |
| detection of porphyromonas gingivalis in gingival exudate by a dipeptide-enhanced trypsin-like activity. | porphyromonas gingivalis in subgingival plaque is an important risk factor for future periodontal attachment loss in susceptible adults. the elimination of p. gingivalis is usually concomitant with a healing process. therefore, it should be valuable to have an easy chairside method to follow the effect of periodontal treatment on p. gingivalis detection as well as on its eventual reappearance during the maintenance period. we have previously reported the stimulation of amidolytic activity of p. ... | 1994 | 8133415 |
| relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults. | to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ... | 1994 | 8157769 |
| flow-cytometric identification and detection of porphyromonas gingivalis by a lps specific monoclonal antibody. | the purpose of this study was to identify porphyromonas gingivalis (p. gingivalis) by flow cytometry (fcm) using a monoclonal antibody (mab) omr-bg1e directed to p. gingivalis-specific lipopolysaccharide (lps). the p. gingivalis strains atcc 33277, 381, eso75, w50, and a7a1 were selected for the study. fusobacterium nucleatum (f. nucleatum), prevotella intermedia (p. intermedia), campylobacter rectus (c. rectus), streptococcus sanguis (s. sanguis) and actinobacillus actinomycetemcomitans (a. act ... | 1994 | 8195974 |
| impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis. | the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ... | 1994 | 8195981 |
| microbial associations in periodontitis sites before and after treatment. | duplicate samples from 110 periodontal sites of 6 mm or more pocket depth in 16 patients were analyzed for the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, capnocytophaga spp., campylobacter rectus, eikenella corrodens and fusobacterium nucleatum. the sites were sampled before and after nonsurgical periodontal treatment. no statistically significant associations were found before treatment between any of the analyzed species. after treatment, ... | 1993 | 8247608 |