| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| outer membrane components of campylobacter hyointestinalis. | outer membranes were isolated, by sodium lauryl sulphate extraction, from the american type strain, five australian, and four english isolates of campylobacter hyointestinalis. on sds-page examination, the protein profiles of seven strains (including the type strain) were similar, and were dominated by two major proteins of 47 and 50 kda. three other isolates had unique major protein profiles. the largest of these proteins was heat-modifiable in these isolates, and in the type strain. the flagel ... | 1992 | 1624127 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| oligodeoxynucleotide probes for campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequences. | deoxyoligonucleotide probes were constructed for the identification of campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequence data. probes were targeted to hypervariable regions of 16s rrna. specificity of oligonucleotide probes was tested in a colony blot assay with type strains of 15 campylobacter and arcobacter species as well as in a slot blot format using genomic dna extracted from field strains of c. fetus and c. hyointestinalis. two oligonucleotides were construc ... | 1991 | 1723076 |
| use of embryonating eggs for isolation of campylobacter species from intestines of swine with proliferative enteritis. | intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. at 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for campylobacter species. campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). campylobacter ... | 1991 | 1883083 |
| immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis. | antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ... | 1990 | 2099622 |
| effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates. | coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ... | 1990 | 2156779 |
| morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, alopex lagopus. | in a blue fox farm about 300 of 400 weanling pups were affected with diarrhoea and prolapse of the rectum. about 40 pups died in an emaciated state after a few days of disease. eight pups were submitted for examination. pathological findings were restricted to the caecum, proximal colon and rectum, with occasional extension to the ileum. affected areas revealed a thick and rigid intestinal wall, a narrow lumen and a thickened, wrinkled and sometimes ulcerated mucosa. histologically, the mucosa s ... | 1990 | 2195067 |
| species-specific dna probes for campylobacter species isolated from pigs with proliferative enteritis. | cloned, chromosomal dna probes from porcine isolates of campylobacter hyointestinalis and c. mucosalis were developed for the detection and identification of these putative swine enteric pathogens. high molecular weight chromosomal dna from each species was used to construct genomic libraries in plasmids. recombinants were selected which hybridized strongly to the homologous organism, but not to any other species of campylobacter. species-specific recombinants were labeled with phosphorus-32 and ... | 1990 | 2260274 |
| bovine diarrhea associated with campylobacter hyointestinalis. | an enteric disease affected 16 ruminating calves. the disease was characterized by a nonspecific, mild to severe diarrhea and wasting. two calves died during the course of disease. c. hyointestinalis was isolated from 12 to 14 calves. the antibody titers of affected calves to c. hyointestinalis varied from 1:20 to 1:160. the disease was successfully treated with chloramphenicol. | 1990 | 2363326 |
| species-specific cloned dna probes for the identification of campylobacter hyointestinalis. | conventional microbiological methods for isolating and identifying campylobacter species are laborious, tedious, and subjective. because of the increasing importance of campylobacter species in human and animal diseases and the recent emergence of many new species and atypical strains, we are developing chromosomal dna probes for rapid and simple identification of campylobacter species, especially those of veterinary importance. we report the cloning and characterization of chromosomal dna fragm ... | 1989 | 2592537 |
| new selective media for the isolation of campylobacter mucosalis and campylobacter hyointestinalis. | | 1988 | 3199619 |
| electrophoretic protein patterns in campylobacter species with special reference to campylobacter mucosalis and campylobacter hyointestinalis. | | 1988 | 3210482 |
| the isolation of campylobacter hyointestinalis from a pig in south africa. | the isolation of a strain of campylobacter hyointestinalis from a piglet is described. the animal originated from a farm where another animal showed signs of intestinal adenomatosis. the animal from which the isolation was made had diarrhoea, and an enteropathogenic escherichia coli was also isolated. no pathological changes indicative of intestinal adenomatosis were detected in this animal. | 1988 | 3393372 |
| campylobacter hyointestinalis associated with human gastrointestinal disease in the united states. | campylobacter hyointestinalis was isolated from stool specimens of four persons, all of whom were experiencing nonbloody, watery diarrhea. the youngest (8 months of age) and the oldest (79 years of age) individuals were females, and the other two were homosexual men. c. hyointestinalis was the only clinically significant pathogen isolated from stool specimens for three of the individuals. in case 3 (involving a 37-year-old homosexual man), entamoeba histolytica and shigella sonnei were also pres ... | 1987 | 3571477 |
| in vitro activities of 47 antimicrobial agents against three campylobacter spp. from pigs. | the in vitro activities of 47 antimicrobial agents against 30 isolates of campylobacter species from pigs were determined by the agar dilution technique. the isolates were obtained from pigs with proliferative enteritis and included 10 strains each of campylobacter coli, campylobacter sputorum subsp. mucosalis, and "campylobacter hyointestinalis gebhart et al." (this name is not on the approved lists). carbadox, furazolidone, nitrofurantoin, gentamicin, and dimetridazole were the most active dru ... | 1985 | 3985597 |
| "campylobacter hyointestinalis" sp. nov.: a new species of campylobacter found in the intestines of pigs and other animals. | the name "campylobacter hyointestinalis" sp. nov. is proposed for a campylobacter species that was isolated from the intestines of pigs with proliferative enteritis. "c. hyointestinalis" is also found in the feces of cattle and has been isolated from the intestine of a hamster. "c. hyointestinalis" is distinguished from previously described catalase-positive campylobacter species by colony morphology, ability to produce h2s in triple sugar iron agar, ability to grow anaerobically in 0.1% trimeth ... | 1985 | 3998100 |
| campylobacter sputorum subsp mucosalis and campylobacter hyointestinalis infections in the intestine of gnotobiotic pigs. | at 4 days of age, 7 gnotobiotic pigs were orally inoculated with broth cultures of both campylobacter sputorum subsp mucosalis (csm) and campylobacter hyointestinalis (ch). one pig was killed and evaluated each week for 7 weeks. forty-eight hours after inoculation, ch and csm were recovered from the feces of the pigs; thereafter, only ch was recovered. organisms morphologically typical of campylobacter sp were observed on the mucosal surface and on the crypt epithelial cells of the ileum, cecum, ... | 1985 | 4062022 |
| immunofluorescent demonstration of campylobacter hyointestinalis and campylobacter sputorum subsp mucosalis in swine intestines with lesions of proliferative enteritis. | an indirect fluorescent antibody technique was developed to identify campylobacter spp in lesions of swine proliferative enteritis (spe). rabbit antisera to c hyointestinalis and c sputorum subsp mucosalis were produced. bacterial smears stained by fluorescent antibody test with homologous antisera differentiated c hyointestinalis from subsp mucosalis. ileal frozen sections from 29 pigs with histologic lesions of spe had specific fluorescent staining of c hyointestinalis in all 29 and subsp muco ... | 1984 | 6375483 |
| isolation of campylobacter hyointestinalis from pigs in the united kingdom. | | 1984 | 6474793 |
| comparison of two selective media and a membrane filter technique for isolation of campylobacter species from diarrhoeal stools. | diarrhoeal stool specimens from 415 patients were examined for campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (ccda), skirrow medium and columbia blood agar overlaid with a 0.65 micron pore size membrane filter. forty-eight campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were campylobacter jejuni (91.7%), three were campylobacter coli (6.3%) and one was campylobacter hyointestinalis (2.0%). the percentages of campylobacter-positive specime ... | 1995 | 7588831 |
| campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis. | campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ... | 1995 | 7857809 |
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| characterization of the replication region of the small cryptic plasmid of campylobacter hyointestinalis. | the complete nucleotide sequence of a 2.5-kb cryptic plasmid from campylobacter hyointestinalis was determined. only one open reading frame (orf), encoding a polypeptide of m(r) 39,667, designated repa, could be identified within the sequence. this was confirmed by minicell analysis. analysis of the region upstream from the orf showed an a+t-rich region followed by four 19-bp direct repeats. together, these features are characteristic of other replication origins (ori(s)). the promoter sequence ... | 1993 | 8449408 |
| isolation of a restriction-less mutant and development of a shuttle vector for the genetic analysis of campylobacter hyointestinalis. | a cosmid shuttle cloning vector, pchi15, was constructed which could be mobilized from escherichia coli k-12 to a putative restriction-less mutant of campylobacter hyointestinalis, c. fetus subsp. fetus, and c. fetus subsp. venerealis at a frequency of 10(-4) transconjugants per donor. a previously described c. coli shuttle vector, pill550, could not be mobilized into the c. hyointestinalis restriction-less mutant, implying that the c. coli replicon was not functional in a c. hyointestinalis hos ... | 1993 | 8449409 |
| genetic analysis of the minimal replicon of plasmid pip417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from bacteroides spp. | the nucleotide sequence of the dna replication origin region of a bacteroides vulgatus plasmid, pip417, encoding 5-nitroimidazole resistance has been determined. this region of 1934 bp presents some characteristics similar to those of other replication protein-dependent origins. it contains a large open reading frame which could encode a basic rep protein (repa) of 36.8 kda. upstream of this orf exist an at-rich region, three direct repeats (iterons) of 21 bp, multiple dnaa binding sites, and si ... | 1995 | 8559801 |
| pcr detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples. | three sets of primers were designed for pcr detection and differentiation of campylobacter jejuni and campylobacter coli. the first pcr assay was designed to coidentify c. jejuni and c. coli based on their 16s rrna gene sequences. the second pcr assay, based on the hippuricase gene sequence, identified all tested reference strains of c. jejuni and also strains of that species which lack detectable hippuricase activity. the third pcr assay, based on the sequence of a cloned (putative) aspartokina ... | 1997 | 9316909 |
| epidemiological typing of campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping. | campylobacter spp. are a leading cause of bacterial gastroenteritis. foods of animal origin, particularly under-cooked poultry, are common sources of campylobacter species associated with disease in humans. a collection of 110 campylobacter jejuni and 31 c. coli human and environmental isolates from different ontario, canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. previously collected serotyping data for the same isolates were also ... | 1998 | 9647797 |
| microtiter assay for detecting campylobacter spp. and helicobacter pylori with surface gangliosides which bind cholera toxin. | campylobacter jejuni with gm1 ganglioside in the core of its lipopolysaccharide has been associated with guillain-barré syndrome. since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of gm1 on their surfaces was developed. in the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a ... | 1998 | 9650959 |
| the isolation and prevalence of campylobacters from dairy cattle using a variety of methods. | faecal samples from 94 dairy cows and 42 calves in three different herds were examined by a variety of techniques for campylobacters. cefoperazone amphotericin teicoplanin (cat) agar, modified cefoperazone charcoal deoxycholate agar (mccda), karmali agar, and membrane filtration onto blood agar, were used with and without enrichment in cat broth. seventy-nine percent of cattle in herd a carried campylobacters, compared with 40% and 37.5% of cattle in herds b and c, respectively. most animals car ... | 1998 | 9674125 |
| identification of the enteropathogens campylobacter jejuni and campylobacter coli based on the cadf virulence gene and its product. | campylobacter jejuni and campylobacter coli are common causes of gastroenteritis in humans. infection with c. jejuni or c. coli is commonly acquired by eating undercooked chicken. the goal of this study was to develop specific detection assays for c. jejuni and c. coli isolates based on the cadf virulence gene and its product. the cadf gene from c. jejuni and c. coli encodes a 37-kda outer membrane protein that promotes the binding of these pathogens to intestinal epithelial cells. a fragment of ... | 1999 | 9986804 |
| extensive 16s rrna gene sequence diversity in campylobacter hyointestinalis strains: taxonomic and applied implications. | phylogenetic relationships of campylobacter hyointestinalis subspecies were examined by means of 16s rrna gene sequencing. sequence similarities among c. hyointestinalis subsp. lawsonii strains exceeded 99.0%, but values among c. hyointestinalis subsp. hyointestinalis strains ranged from 96.4 to 100%. sequence similarities between strains representing the two different subspecies ranged from 95.7 to 99.0%. an intervening sequence was identified in certain of the c. hyointestinalis subsp. lawsoni ... | 1999 | 10425776 |
| large-scale survey of campylobacter species in human gastroenteritis by pcr and pcr-enzyme-linked immunosorbent assay. | a pcr-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening pcrs and species identification by pcr-enzyme-linked immunosorbent assay. this was applied to dna extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional public health laboratories in england and wales over a 2-year period. the sending laboratories had c ... | 1999 | 10565897 |
| rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene. | a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay. | 1999 | 10565952 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| genotyping of campylobacter spp. | | 2000 | 10618195 |
| isolation and characterization of campylobacter jejuni subsp. jejuni from macaroni penguins (eudyptes chrysolophus) in the subantarctic region. | on bird island, south georgia, albatrosses (n = 140), penguins (n = 100), and fur seals (n = 206) were sampled for campylobacter jejuni. c. jejuni subsp. jejuni was recovered from three macaroni penguins (eudyptes chrysolophus). these isolates, the first reported for the subantarctic region, showed low genetic diversity and high similarity to northern hemisphere c. jejuni isolates, possibly suggesting recent introduction to the area. | 2000 | 10618265 |
| identification of campylobacter jejuni, c. coli, c. lari, c. upsaliensis, arcobacter butzleri, and a. butzleri-like species based on the glya gene. | currently, the detection and identification of campylobacter and arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. we have developed a pcr-hybridization strategy, wherein degenerate primers are used to amplify glya fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between campylobacter jejuni, c. coli, c. ... | 2000 | 10747131 |
| campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir. | campylobacter-like organisms were isolated from the faeces of healthy individuals during a hygiene survey of abattoir workers. the strains, which exhibited characteristics of campylobacter, being non-glucose-fermenting, oxidase- and catalase-positive, gram-negative, motile rods, were identified to the genus level by a pcr assay. nucleotide sequence analysis of the 16s rrna gene, dna homology experiments and determination of g + c content demonstrated that they constituted a previously undescribe ... | 2000 | 10758898 |
| isolation and characterization of strains cvo and fwko b, two novel nitrate-reducing, sulfide-oxidizing bacteria isolated from oil field brine. | bacterial strains cvo and fwko b were isolated from produced brine at the coleville oil field in saskatchewan, canada. both strains are obligate chemolithotrophs, with hydrogen, formate, and sulfide serving as the only known energy sources for fwko b, whereas sulfide and elemental sulfur are the only known electron donors for cvo. neither strain uses thiosulfate as an energy source. both strains are microaerophiles (1% o(2)). in addition, cvo grows by denitrification of nitrate or nitrite wherea ... | 2000 | 10831429 |
| misidentifying helicobacters: the helicobacter cinaedi example. | whole-cell protein electrophoresis and biochemical examination by means of a panel of 64 tests were used to identify 14 putative helicobacters to the species level. the results were confirmed by means of dna-dna hybridization experiments and were used to discuss misidentification of helicobacters based on 16s rrna gene sequence data. the data indicated that comparison of near-complete 16s ribosomal dna sequences does not always provide conclusive evidence for species level identification and may ... | 2000 | 10834986 |
| evaluation of phenotypic and genotypic methods for subtyping campylobacter jejuni isolates from humans, poultry, and cattle. | six methods for subtyping of campylobacter jejuni were compared and evaluated with a collection of 90 isolates from poultry, cattle, and sporadic human clinical cases as well as from a waterborne outbreak. the applied methods were penner heat-stable serotyping; automated ribotyping (riboprinting); random amplified polymorphic dna typing (rapd); pulsed-field gel electrophoresis (pfge); restriction fragment length polymorphisms of the flagellin gene, flaa (fla-rflp); and denaturing gradient gel el ... | 2000 | 11015406 |
| identification of ciprofloxacin-resistant campylobacter jejuni by use of a fluorogenic pcr assay. | fluoroquinolones are one class of antimicrobial agents commonly used to treat severe campylobacter jejuni infection. c. jejuni strains resistant to high levels of the fluoroquinolone ciprofloxacin (mic >/=16 microg/ml) have been predominantly characterized with a c-->t transition in codon 86 of gyra. the gyra gene encodes one subunit of dna gyrase, which is a primary target for fluoroquinolone antibiotics. this study establishes a rapid pcr-based taqman method for identifying ciprofloxacin-resis ... | 2000 | 11060054 |
| identification of taxonomic and epidemiological relationships among campylobacter species by numerical analysis of aflp profiles. | amplified fragment length polymorphism (aflp)-based profiling was performed on 138 strains representing all named campylobacter species and subspecies. profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. the mean similarity of 48 duplicate, outbreak and/or 'identical' strain profiles exceeded 94%. species were clearly distinguished at the 17.90% similarity (s-) level in the dendrogram. subspecies of campylobacter jejuni and campylobacter ... | 2000 | 11094296 |
| rapid pulsed-field gel electrophoresis protocol for subtyping of campylobacter jejuni. | we developed a rapid pulsed-field gel electrophoresis (pfge) protocol for subtyping campylobacter isolates based on the standardized protocols used by pulsenet laboratories for the subtyping of other food-borne bacterial pathogens. various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, r ... | 2001 | 11326009 |
| comparative study using amplified fragment length polymorphism fingerprinting, pcr genotyping, and phenotyping to differentiate campylobacter fetus strains isolated from animals. | a collection of campylobacter fetus strains, including both c. fetus subsp. fetus and c. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by pcr and amplified fragment length polymorphism (aflp) analysis. phenotypic subspecies determination methods were unreliable. genotyping of the strains by pcr and aflp showed a clear discrimination between the two subspecies. | 2001 | 11376071 |
| genomic heterogeneity and o-antigenic diversity of campylobacter upsaliensis and campylobacter helveticus strains isolated from dogs and cats in germany. | a serotyping scheme based on heat-stable surface antigens was established for 101 campylobacter upsaliensis and 10 campylobacter helveticus strains isolated from 261 dogs and 46 cats of different ages originating from two geographically distinct regions in germany. the prevalence of c. upsaliensis varied between 27.8% in juvenile dogs (<12 months of age) and 55.4% in adult dogs (p < 0.05). of the cats, 19.6% harbored c. upsaliensis, whereas 21.7% carried c. helveticus. of the c. upsaliensis isol ... | 2001 | 11427567 |
| differentiation of campylobacter species by aflp fingerprinting. | the fluorescent amplified fragment length polymorphism (aflp) fingerprinting method was tested for its ability to identify and subtype the most important campylobacter species found in veterinary infections. sixty-nine reference strains and 19 clinical isolates of campylobacter jejuni subsp. jejuni, campylobacter jejuni subsp. doylei, campylobacter upsaliensis, campylobacter coli, campylobacter lari, campylobacter fetus subsp. fetus, c. fetus subsp. venerealis, campylobacter hyointestinalis subs ... | 2001 | 11577152 |
| efficient isolation of campylobacter upsaliensis from stools. | | 2001 | 11712518 |
| evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque. | a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ... | 2002 | 11773117 |
| campylobacter hyointestinalis subsp. hyointestinalis, a common campylobacter species in reindeer. | to study the prevalence of campylobacter spp. in the faecal material of reindeer, and to identify the isolates by means of a polyphasic approach. in addition, to study the genetic diversity of camp. hyointestinalis subsp. hyointestinalis reindeer isolates by pulsed-field gel electrophoresis (pfge). | 2002 | 11966912 |
| development and application of a new scheme for typing campylobacter jejuni and campylobacter coli by pcr-based restriction fragment length polymorphism analysis. | a molecular typing approach for campylobacter jejuni and campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb pcr-amplified portion of the lipopolysaccharide gene cluster. sixty-one penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. eleven additional genogroups were obtained from 87 clinical c. jejuni strains tested. this molecular typing method shows a correlation with the penner heat-stab ... | 2002 | 11980961 |
| transmission of campylobacter hyointestinalis from a pig to a human. | we report on a case of human gastroenteritis caused by the pathogen campylobacter hyointestinalis. recurrent watery diarrhea and intermittent vomiting were the most significant symptoms of the previously healthy patient. whole-cell protein electrophoresis and 16s rrna gene sequencing were used to identify this campylobacter species. investigation of the patient's surroundings led to the recovery of a second c. hyointestinalis strain originating from porcine feces. subsequent typing of the human ... | 2002 | 12089284 |
| dna sequence and mutational analyses of the pvir plasmid of campylobacter jejuni 81-176. | the circular pvir plasmid of campylobacter jejuni strain 81-176 was determined to be 37,468 nucleotides in length with a g+c content of 26%. a total of 83% of the plasmid represented coding information, and all but 2 of the 54 predicted open reading frames were encoded on the same dna strand. there were seven genes on the plasmid in a continguous region of 8.9 kb that encoded orthologs of type iv secretion proteins found in helicobacter pylori, including four that have been described previously ... | 2002 | 12379703 |
| prevalence of campylobacter jejuni, campylobacter lari, and campylobacter coli in different ecological guilds and taxa of migrating birds. | a total of 1,794 migrating birds trapped at a coastal site in southern sweden were sampled for detection of campylobacter spp. all isolates phenotypically identified as campylobacter jejuni and a subset of those identified as non-c. jejuni were identified to the species level by pcr-based techniques. c. jejuni was found in 5.0% of the birds, campylobacter lari was found in 5.6%, and campylobacter coli was found in 0.9%. an additional 10.7% of the tested birds were infected with hippurate hydroly ... | 2002 | 12450810 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| specific detection of arcobacter and campylobacter strains in water and sewage by pcr and fluorescent in situ hybridization. | the aim of this study was to evaluate pcr and fluorescent in situ hybridization (fish) techniques for detecting arcobacter and campylobacter strains in river water and wastewater samples. both 16s and 23s rrna sequence data were used to design specific primers and oligonucleotide probes for pcr and fish analyses, respectively. in order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on ... | 2003 | 12571045 |
| characterization of campylobacter lanienae from pig feces. | to isolate campylobacter spp., the feces of healthy cattle, pigs, and broilers were examined between june 1999 and january 2000. campylobacter lanienae strains were isolated from the feces of healthy pigs, but not from the feces of cattle or broilers. in six c. lanienae isolates, there was only 21-38% dna-dna homology to campylobacter hyointestinalis subsp. lawsonii strain nctc 12901. thus, the primary host of c. lanienae is likely to be the pig and c. lanienae appears to be a species distinct f ... | 2003 | 12576719 |
| a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture. | a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ... | 2003 | 12620820 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| avian reservoirs and zoonotic potential of the emerging human pathogen helicobacter canadensis. | a polyphasic identification approach was used to investigate the taxonomic position of campylobacter-like isolates recovered from barnacle geese (branta leucopsis) and canada geese (branta candensis). seven strains were selected from a collection of 21 isolates and analyzed by extensive phenotypic testing; four strains were characterized by 16s rrna gene sequence analysis. the results clearly identified the bird isolates as helicobacter canadensis, recently described as an emerging human pathoge ... | 2003 | 14660407 |
| a survey of campylobacter species shed in faeces of beef cattle using polymerase chain reaction. | a polymerase chain reaction (pcr)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. to ensure the removal of pcr inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control dna (i.e., dna designed to amplify with the campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. in only two samples (0.5%) were the internal control or campylobacter genu ... | 2003 | 14735214 |
| multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis. | helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ... | 2004 | 15004062 |
| direct quantification of campylobacter jejuni and campylobacter lanienae in feces of cattle by real-time quantitative pcr. | campylobacter species are fastidious to culture, and the ability to directly quantify biomass in microbiologically complex substrates using real-time quantitative (rtq) pcr may enhance our understanding of their biology and facilitate the development of efficacious mitigation strategies. this study reports the use of nested rtq-pcr to directly quantify campylobacter jejuni and campylobacter lanienae in cattle feces. for c. jejuni, the single-copy mapa gene was selected. for c. lanienae, the thre ... | 2004 | 15066825 |
| frequency and spatial distribution of environmental campylobacter spp. | humans are exposed to campylobacter spp. in a range of sources via both food and environmental pathways. for this study, we explored the frequency and distribution of thermophilic campylobacter spp. in a 10- by 10-km square rural area of cheshire, united kingdom. the area contains approximately 70, mainly dairy, farms and is used extensively for outdoor recreational activities. campylobacter spp. were isolated from a range of environmental samples by use of a systematic sampling grid. livestock ... | 2004 | 15528512 |
| detection of campylobacter spp. in chicken fecal samples by real-time pcr. | a real-time pcr assay for detecting thermophilic campylobacter spp. directly in chicken feces has been developed. dna was isolated from fecal material by using magnetic beads followed by pcr with a prealiquoted pcr mixture, which had been stored at -18 degrees c. campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 cfu/ml, in a fecal suspension. a bacterial internal control was added before dna extraction to control both dna isolation and the presence of pcr inh ... | 2004 | 15528705 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| susceptibility of campylobacter hyointestinalis subsp. hyointestinalis to antimicrobial agents and characterization of quinolone-resistant strains. | to study the susceptibility of campylobacter hyointestinalis subsp. hyointestinalis to several antimicrobial agents and to investigate the mechanisms of nalidixic acid and ciprofloxacin resistance. | 2005 | 15649999 |
| prevalence and genetic diversity of campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area. | water samples were taken systematically from a 100-km2 area of mainly dairy farmland in northwestern england and examined for campylobacter spp. pulsed-field gel electrophoresis-restriction fragment length polymorphism (pfge-rflp) and flaa strain typing of campylobacter jejuni and campylobacter coli isolates were done. data on the water source and the adjacent environment were recorded and examined as explanatory variables. campylobacter spp. were isolated from 40.5% (n = 119) of the water sampl ... | 2005 | 15812015 |
| effects of subtherapeutic administration of antimicrobial agents to beef cattle on the prevalence of antimicrobial resistance in campylobacter jejuni and campylobacter hyointestinalis. | the influence of antimicrobial agents on the development of antimicrobial resistance (amr) in campylobacter isolates recovered from 300 beef cattle maintained in an experimental feedlot was monitored over a 315-day period (11 sample times). groups of calves were assigned to one of the following antimicrobial treatments: chlortetracycline and sulfamethazine (cs), chlortetracycline alone (ct), virginiamycin, monensin, tylosin phosphate, and no antimicrobial agent (i.e., control treatment). in tota ... | 2005 | 16000800 |
| discordant 16s and 23s rrna gene phylogenies for the genus helicobacter: implications for phylogenetic inference and systematics. | analysis of 16s rrna gene sequences has become the primary method for determining prokaryotic phylogeny. phylogeny is currently the basis for prokaryotic systematics. therefore, the validity of 16s rrna gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. discrepancies between 16s rrna gene analyses and dna-dna hybridization and phenotypic analyses have been noted in the genus helicobacter. to clarify these discrepancies, we sequenced the 23s rrna genes for ... | 2005 | 16109952 |
| validation of a monoclonal antibody-based capture enzyme-linked immunosorbent assay for detection of campylobacter fetus. | a monoclonal antibody (mab)-based antigen capture enzyme-linked immunosorbent assay (elisa) was compared with the routine culture methodology for the detection of campylobacter fetus subspecies from bovine and ovine field samples inoculated into clark's transport enrichment medium (tem). the work was a collaboration between two different diagnostic laboratories, one in canada and the other in england. in both labs, tem samples were incubated for 4 days at 35 degrees c and then tested by culture ... | 2005 | 16275938 |
| comparison of culture and a novel 5' taq nuclease assay for direct detection of campylobacter fetus subsp. venerealis in clinical specimens from cattle. | a campylobacter fetus subsp. venerealis-specific 5' taq nuclease pcr assay using a 3' minor groove binder-dna probe (taqman mgb) was developed based on a subspecies-specific fragment of unknown identity (s. hum, k. quinn, j. brunner, and s. l. on, aust. vet. j. 75:827-831, 1997). the assay specifically detected four c. fetus subsp. venerealis strains with no observed cross-reaction with c. fetus subsp. fetus-related campylobacter species or other bovine venereal microflora. the 5' taq nuclease a ... | 2006 | 16517880 |
| temporal prevalence of antimicrobial resistance in campylobacter spp. from beef cattle in alberta feedlots. | antimicrobial resistance (amr) was temporally assessed in campylobacters isolated from beef cattle (7,738 fecal samples from 2,622 animals) in four commercial feedlots in alberta. all calves were administered chlortetracycline and oxytetracycline in feed, and a majority of the animals (93%) were injected with long-acting oxytetracycline upon arrival at the feedlot. fecal samples from individual animals were collected upon arrival (i.e., entry sample), 69 days (standard deviation [sd] = 3 days) a ... | 2006 | 16751519 |
| atypical helicobacter canadensis strains associated with swine. | forty-two helicobacter isolates were isolated from swine feces in the netherlands and denmark. all 12 isolates sequenced (16s rrna gene) formed a robust clade with helicobacter canadensis ( approximately 99% similarity). species-specific pcr indicated that all of the isolates were h. canadensis isolates. although the appearance of the porcine isolates was similar to the appearance of h. canadensis, only one of these isolates was able to hydrolyze indoxyl acetate, a cardinal characteristic of thi ... | 2006 | 16751570 |
| intestinal carriage of campylobacter jejuni and campylobacter coli among cattle from south-western norway and comparative genotyping of bovine and human isolates by amplified-fragment length polymorphism. | in a survey conducted in 1999-2001, the carriage of thermotolerant campylobacters in cattle was investigated, and the genetic diversity of c. jejuni within one herd was examined and compared with human isolates. c. jejuni, c. coli and other thermotolerant campylobacter spp. were isolated from intestinal contents from 26%, 3% and 2% of 804 cattle, respectively. the carriage rate was higher in calves (46%) than in adults (29%). twenty-nine c. jejuni isolates from one herd and 31 human isolates fro ... | 2006 | 16987400 |
| campylobacter spp., enterococcus spp., escherichia coli, salmonella spp., yersinia spp., and cryptosporidium oocysts in semi-domesticated reindeer (rangifer tarandus tarandus) in northern finland and norway. | the specific aim of this study was to assess the faecal shedding of zoonotic enteropathogens by semi-domesticated reindeer (rangifer tarandus tarandus) to deduce the potential risk to human health through modern reindeer herding. in total, 2,243 faecal samples of reindeer from northern regions of finland and norway were examined for potentially enteropathogenic bacteria (campylobacter species, enterococcus species, escherichia coli, salmonella species and yersinia species) and parasites (cryptos ... | 2006 | 16987403 |
| prevalence of campylobacter spp. in cattle in finland and antimicrobial susceptibilities of bovine campylobacter jejuni strains. | the study investigated the prevalence of campylobacter spp. in finnish cattle at slaughter and carcass contamination after slaughter. during the period january to december 2003, bovine rectal fecal samples (n=952) and carcass surface samples (n=948) from 12 out of 15 finnish slaughterhouses were examined. in total, campylobacters were detected in 31.1% of fecal samples and in 3.5% of carcass surface samples. campylobacter jejuni was isolated from 19.5%, campylobacter coli from 2.2%, and presumpt ... | 2007 | 17369335 |
| influence of ph of tsi medium on the detection of hydrogen sulfide production by campylobacter hyointestinalis. | to examine the influence of ph of triple sugar iron (tsi) agar medium on the detection of hydrogen sulfide (h(2)s) production in campylobacter hyointestinalis ssp. hyointestinalis (chh). | 2007 | 17451523 |
| development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
| development of a multiplex pcr assay for identification of campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. | a multiplex pcr assay has been developed for the identification of the six common campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. the assay was developed using a combination of newly designed and published primers. it provided a specific pcr product for each of the five campylobacter species and ... | 2007 | 17965346 |
| development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations. | the identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. a novel molecule-based method, using strain- and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. strain-specific oligo ... | 2008 | 18281428 |
| evaluation of 16s rrna gene-based pcr assays for genus-level identification of helicobacter species. | the inclusivity, exclusivity, and detection limit of six 16s rrna gene-based helicobacter genus-specific pcr assays were examined. five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). the clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture. | 2008 | 18337392 |
| molecular epidemiology and characterization of campylobacter spp. isolated from wild bird populations in northern england. | campylobacter infections have been reported at prevalences ranging from 2 to 50% in a range of wild bird species, although there have been few studies that have investigated the molecular epidemiology of campylobacter spp. consequently, whether wild birds are a source of infection in humans or domestic livestock or are mainly recipients of domestic animal strains and whether separate cycles of infection occur remain unknown. to address these questions, serial cross-sectional surveys of wild bird ... | 2009 | 19286781 |
| diversity of 23s rrna genes within individual prokaryotic genomes. | the concept of ribosomal constraints on rrna genes is deduced primarily based on the comparison of consensus rrna sequences between closely related species, but recent advances in whole-genome sequencing allow evaluation of this concept within organisms with multiple rrna operons. | 2009 | 19415112 |
| prolonged survival of campylobacter species in bovine manure compost. | the persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (as700) or from cattle not administered antibiotics (control) was examined. although there were no differences in population sizes of heterotrophic bacteria, the temperature of as700 compost was more variable and did not become as high as that of control compost. there were significant differences in water content, total carb ... | 2010 | 20023098 |
| amoebae and algae can prolong the survival of campylobacter species in co-culture. | several species of free-living amoebae can cause disease in humans. however, in addition to the direct pathogenicity of e.g. acanthamoebae and naegleria species, they are recognized as environmental hosts, indirectly involved in the epidemiology of many pathogenic bacteria. although several studies have demonstrated intracellular survival of many different bacteria in these species, the extent of such interactions as well as the implications for the epidemiology of the bacterial species involved ... | 2010 | 20056117 |
| three-hour molecular detection of campylobacter, salmonella, yersinia, and shigella species in feces with accuracy as high as that of culture. | campylobacter jejuni and salmonella, shigella, and yersinia species (along with shiga toxin-producing escherichia coli) are the most common causes of acute bacterial diarrheal disease in the united states. current detection techniques are time-consuming, limiting usefulness for patient care. we developed and validated a panel of rapid pcr assays for the detection and identification of c. jejuni, c. coli, salmonella, and yersinia species and shigella and enteroinvasive e. coli in stool samples. a ... | 2010 | 20519461 |
| obstacles of multiplex real-time pcr for bacterial 16s rdna: primer specifity and dna decontamination of taq polymerase. | background: the detection of a broad range of bacteria by pcr is applied for the screening of blood and blood products with special attention to platelet concentrates. for practical use it is desirable that detection systems include gram-positive, gram-negative and non-gram-stainable bacteria. it is quite challenging to achieve high sensitivity along with a clear negative control with pcr reagents, because especially taq polymerase is contaminated with traces of bacterial dna. methods: bacterial ... | 2010 | 20737013 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| enteric campylobacteria and rna viruses associated with healthy and diarrheic humans in the chinook health region of southwestern alberta, canada. | the presence of campylobacter species and enteric rna viruses in stools from diarrheic (n = 442) and healthy (n = 58) humans living in southwestern alberta was examined (may to october 2005). a large number of diarrheic individuals who were culture negative for c. jejuni (n = 54) or c. coli (n = 19) were pcr positive for these taxa. overall detection rates for c. jejuni and c. coli in diarrheic stools were 29% and 5%, respectively. in contrast, 3% and 0% of stools from healthy humans were positi ... | 2010 | 21106791 |
| campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (pan troglodytes schweinfurthii) in tanzania. | the transmission of simian immunodeficiency and ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. in this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (pan troglodytes) populations with different histories in relation to their proximity to humans. campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research ... | 2011 | 21278267 |
| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| emerging thermotolerant campylobacter species in healthy ruminants and swine. | campylobacters other than campylobacter jejuni or c. coli were isolated in 35% of 343 farms recently analyzed in northern spain. this study was aimed at identifying at the species level the 120 isolates collected (21 ovine, 52 beef cattle, 44 dairy cattle, and 3 porcine) by species-specific polymerase chain reaction and 16s rrna gene sequencing analysis. thus, five species were identified: campylobacter hyointestinalis (90 isolates), campylobacter lanienae (12), campylobacter fetus subsp. fetus ... | 2011 | 21438765 |
| rapid identification and quantification of campylobacter coli and campylobacter jejuni by real-time pcr in pure cultures and in complex samples. | abstract: background: campylobacter spp., especially campylobacter jejuni (c. jejuni) and campylobacter coli (c. coli), are recognized as the leading human foodborne pathogens in developed countries. livestock animals carrying campylobacter pose an important risk for human contamination. pigs are known to be frequently colonized with campylobacter, especially c. coli, and to excrete high numbers of this pathogen in their faeces. molecular tools, notably real-time pcr, provide an effective, rapid ... | 2011 | 21600037 |
| Simultaneous Detection of Six Diarrhea-Causing Bacterial Pathogens with an In-House PCR-Luminex Assay. | Diarrhea can be caused by a range of pathogens, including several bacteria. Conventional diagnostic methods, such as culture, biochemical tests, and enzyme-linked immunosorbent assay (ELISA), are laborious. We developed a 7-plex PCR-Luminex assay to simultaneously screen for several of the major diarrhea-causing bacteria directly in fecal specimens, including pathogenic Aeromonas, Campylobacter jejuni, Campylobacter coli, Salmonella, Shigella, enteroinvasive Escherichia coli (EIEC), Vibrio, and ... | 2012 | 22075596 |
| emerging dynamics of human campylobacteriosis in southern ireland. | infections with campylobacter spp. pose a significant health burden worldwide. the significance of campylobacter jejuni/campylobacter coli infection is well appreciated but the contribution of non-c. jejuni/c. coli spp. to human gastroenteritis is largely unknown. in this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the microbiology department in cork university hospital during 2009. the first step, using entericbio(®) (serosep), a multiplex pcr syst ... | 2011 | 22077228 |
| prevalence of campylobacter species in adult crohn's disease and the preferential colonization sites of campylobacter species in the human intestine. | crohn's disease (cd) and ulcerative colitis (uc) are the two major forms of inflammatory bowel disease (ibd). a high prevalence of campylobacter concisus was previously detected in paediatric cd and adult uc. currently, the prevalence of c. concisus in adult cd and the preferential colonization sites of campylobacter species in the human intestine are unknown. in this study, we examined the prevalence of campylobacter species in biopsies collected from multiple anatomic sites of adult patients w ... | 2011 | 21966525 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |