meningism associated with campylobacter jejuni enteritis. 197986821
campylobacter jejuni post-transfusional septicaemia. 197991012
studies of the microaerophilic nature of campylobacter fetus subsp. jejuni. ii. role of exogenous superoxide anions and hydrogen peroxide.the addition of bovine superoxide dismutase to brucella broth or brucellar agar greatly echanced the oxygen tolerance of campylobacter fetus subsp. jejuni strain h840 (atcc 29428). catalase also enhanced oxygen tolerance, but to a lesser extent. these enzymes must act externally to the bacteria. all of the diverse compounds which enhance oxygen tolerance of c. fetus, including nor-epinephrine and a combination of ferrous sulfate, sodium metabisulfite, and sodium pyruvate, share the ability to qu ...1979218715
isolation of campylobacter fetus from recent cases of human vibriosis.campylobacter fetus was isolated from five recent cases of human vibriosis, of which two were adults and three were children. one adult presented with pericarditis and the other with recurrent pyrexia. campylobacter fetus subsp. intestinalis which resembled cattle strains serologically, was isolated under co2 or anaerobic conditions from blood cultures of these patients. two of the three children had kwashiorkor and the third was only 8 days old. isolates identified as campylobacter fetus subsp. ...1977270524
campylobacter enteritis: a common cause of adult diarrhoea.a case of acute diarrhoeal disease in a young male due to campylobacter jejuni is described, and the importance of this organism as a cause of acute diarrhoea in adults is discussed.1979292869
campylobacter enteritis and yersinia enterocolitica infection in new zealand.a three-month survey was undertaken to determine the incidence of campylobacter jejuni and yersinia enterocolitica in diarrhoeal disease and acute abdominal disease in palmerston north. c. jejuni was isolated from five domiciliary patients and one hospitalised patient with acute diarrhoea but there were no isolations from patients suffering from acute abdominal disease. the isolation rates for c. jejuni in domiciliary and hospitalised patients with acute diarrhoea were 7.8 percent and 1.7 percen ...1979292870
studies of the microaerophilic nature of campylobacter fetus subsp. jejuni. i. physiological aspects of enhanced aerotolerance. 1979427650
detection of enteric campylobacteriosis in children.campylobacter fetus subsp. jejuni was recovered as the sole bacterial pathogen from 31% of 0- to 8-month-old children with acute gastroenteritis and from 5% of asymptomatic children (p less than 0.05). in children 8 to 24 months old, the respective recovery rates were 38 and 40%. with the exception of one case of simultaneous bacteremia, the clinical course of the symptomatic infection was benign and rarely lasted more than a week. the isolates were sensitive to most commonly used antibiotics. s ...1979429543
campylobacter enteritis.campylobacter jejuni/coli has recently become recognized as a common bacterial cause of diarrhea. infection can occur at any age. the usual incubation period of campylobacter enteritis is 2 to 5 days. fever, diarrhea and abdominal pain are the most common clinical features. the stools frequently contain mucus and, a few days after the onset of symptoms, frank blood. significant vomiting and dehydration are uncommon. a rapid presumptive laboratory diagnosis may be made during the acute phase of t ...1979455209
brief note. campylobacter jejuni gastroenteritis in brisbane. 1979460954
electron microscopy of campylobacter jejuni.stools from 56 patients with gastroenteritis were cultured for campylobacter jejuni. the five strains isolated were examined by electron microscopy. the campylobacter cells were pleomorphic and most displayed appearances similar to those of v. fetus. morphological changes were observed in cultures subjected to prolonged incubation.1979469932
susceptibility of campylobacter fetus subsp. jejuni to twenty antimicrobiol hundred recent clinical isolates of campylobacter fetus subsp. jejuni were tested by an agar dilution technique for susceptibility to each of 20 antimicrobial agents. doxycycline and gentamicin were the most active of the drugs examined, inhibiting all strains at concentrations achievable in serum. although the median minimal inhibitory concentration of erythromycin was low, 8% of the isolates were highly resistant. all isolates of campylobacter fetus subsp. jejuni were relatively resistant ...1979475372
campylobacter jejuni in a male homosexual. 1979509193
enteritis and septicaemia due to campylobacter jejuni. 1979554396
susceptibility of campylobacter fetus subsp. jejuni to twenty-nine antimicrobial agents.the activity of 29 antimicrobial agents was tested against 95 strains of campylobacter fetus subsp. jejuni isolated from human stools. furazolidone and gentamycin were the most active agents. the tetracyclines, erythromycin, and chloramphenicol were very active against most of the strains, but with each antibiotic a few resistant strains were found. the penicillins were relatively inactive, and the cephalosporins tested were only active against occasional strains.1978718153
campylobacter enteritis in south australia.campylobacter jejuni was isolated on coproculture from 13 of 224 patients with suspected infectious diarrhoea whose faecal specimens were examined during a three-month period. these organisms were not isolated from 530 specimens received from asymptomatic and healthy individuals. diarrhoea and cramping abdominal pain generally lasting two to three days were the major symptoms, though occasional patients had a more prolonged illness. ingestion of campylobacter resulted in enteric infection with i ...1978732725
reactive arthritis associated with campylobacter jejuni enteritis. 1979761021
stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of spirillum volutans and campylobacter fetus subspecies jejuni.the aerotolerance of the microaerophilic bacterium spirillum volutans was greatly stimulated in a defined medium by the presence of dihydroxyphenyl ferric iron-binding compounds such as nor-epinephrine at 10(-5) to 10(-6) m. dihydroxyphenyl compounds at 2 x 10(-4) m, or iron salts (ferrous or ferric) at high concentration, greatly increased the aerotolerance of a strain of campylobacter fetus subsp. jejuni when grown on streak plates of brucella agar. the results suggest that the microaerophilis ...1976942201
rapid and sensitive detection of campylobacter spp. in chicken products by using the polymerase chain reaction.the polymerase chain reaction (pcr) after a short enrichment culture was used to detect campylobacter spp. in chicken products. after the 16s rrna gene sequence of campylobacter jejuni was determined and compared with known sequences from other enterobacteria, a primer and probe combination was selected from the region before v3 and the variable regions v3 and v5. with this primer set and probe, 426-bp fragments from c. jejuni, campylobacter coli, and campylobacter lari could be amplified. the d ...19921282312
the surveillance and control of campylobacter infection.campylobacter jejuni and c. coli are the most commonly reported bacterial causes of enteritis in man in the united kingdom. the reported incidence of campylobacter infection varies with place and time. its epidemiology differs from that of salmonella: campylobacters do not multiply on food, secondary spread is rare, and infections peak at the end of may (salmonellas peak in july). known sources of infection include poultry, milk, water, pets and other domestic animals. campylobacters are widespr ...19921284935
emergence of resistance to erythromycin and fluoroquinolones in thermotolerant campylobacter strains isolated from feces 1987-1991.during the period 1987 to 1991 a retrospective study was performed to determine the resistance of thermotolerant campylobacter species isolated from feces to erythromycin and fluoroquinolones. of the 672 strains studied, 614 (91.3%) were identified as campylobacter jejuni and 58 (8.7%) as campylobacter coli. during the study period the rate of resistance of campylobacter jejuni to erythromycin remained relatively stable (0.9-3.5%), while resistance of campylobacter coli to erythromycin emerged l ...19921291314
campylobacter jejuni in acute diarrhoea in infancy and its relation to faecal leucocytic count.a prospective study was carried out to identify the relative risk of campylobacter jejuni infection in 50 infants with acute diarrhoea, 24 infants with acute resistant diarrhoea and 25 healthy normal infants as a matching control group. faecal samples were collected from the three groups and were cultured on both selective media for campylobacter and other media for isolation of other organisms. direct stool smears, stained with methylene blue, were examined for detection of faecal leucocyte in ...19921295942
characteristics of the internalization and intracellular survival of campylobacter jejuni in human epithelial cell cultures.the characteristics associated with the internalization and intracellular behavior of campylobacter jejuni during short-term and long-term cultivation with int 407 cells were examined. the internalization of c. jejuni by int 407 cells was inhibited by cytochalasin dansylcadaverine, chemicals that disrupt microfilament formation and inhibit receptor cycling, respectively. ammonium chloride and methylamine, two chemicals that inhibit endosomal acidification, did not affect c. jejuni internalizatio ...19921297914
in vivo effect of cadmium chloride on intestinal colonization of rats by campylobacter jejuni.campylobacter jejuni (10(8) c.f.u) fed intragastrically unto 18 laboratory rats (white whiskers) resulted in colonization of the 18 rats intestines and their eventual excretion of the organism for four months post infection. when 10(8) cfu of c. jejuni was mixed with 10 mu/ml of cadmium chloride (cdcl2) and fed immediately to another set of 18 rats, colonization of rats intestines was greatly reduced and c. jejuni was recovered from two (11%) out of 18 rats and the faeces were positive for c. je ...19921298614
[studies of outer membrane protein profiles by sds-page for campylobacter jejuni in an epidemiological investigation].the characteristics of outer membrane protein profiles of 41 strains of campylobacter jejuni from various sources by sds-page was studied. seven and nine omp patterns were differentiated respectively by the presence or absence of six outer membrane protein bands and by the number and size of the molecular weight of the major protein bands. comparing the omp patterns of the strains from human with those from animals, the authors inferred that chickens and other animals might be one of the sources ...19921298718
[advances in the research on the pathogenic role of campylobacter jejuni in guillain-barre syndrome]. 19921305004
colonization of infant mice with flagellar variants of campylobacter jejuni.the role of flagella in the colonization of the intestine by campylobacter jejuni was investigated by challenging infant mice with two flagellated strains and their nonflagellated variants. the intestinal tracts of infant mice were regularly colonized with motile strains, but not by nonmotile variants. colonization of mice with motile c. jejuni occurred with as few as 1000 bacteria per mouse.19921307436
[result of the refinement of a technique for the isolation and identification of campylobacter from food commodities].campylobacter jejuni has been researched in raw milk, skin and excrement of chicken. all milk samples are negatives but 66% of chicken skin and 12% of chicken excrements are positives. an enrichment phase using brucella broth added with horse blood and antibiotics is necessary.19921309139
detection of ciprofloxacin resistance in gram-negative bacteria due to alterations in gyra.two plasmids containing the cloned escherichia coli wild-type gyra gene were used to transform ciprofloxacin-resistant gram-negative clinical isolates to screen for dna gyrase a-mediated quinolone resistance. the results show that the technique is simple and applicable to a wide range of gram-negative species including e. coli, enterobacter cloacae, klebsiella aerogenes, morganella morganii, proteus mirabilis, pseudomonas aeruginosa, campylobacter jejuni and neisseria gonorrhoeae. the use of an ...19921310671
genome maps of campylobacter jejuni and campylobacter coli.little information concerning the genome of either campylobacter jejuni or campylobacter coli is available. therefore, we constructed genomic maps of c. jejuni ua580 and c. coli ua417 by using pulsed-field gel electrophoresis. the genome sizes of c. jejuni and c. coli strains are approximately 1.7 mb, as determined by sali and smai digestion (n. chang and d. e. taylor, j. bacteriol. 172:5211-5217, 1990). the genomes of both species are represented by single circular dna molecules, and maps were ...19921313002
high-level quinolone resistance in clinical isolates of campylobacter jejuni.during a recent clinical trial of ciprofloxacin in the therapy of acute diarrhea, two subjects infected with campylobacter jejuni who received ciprofloxacin failed microbiologically and one also failed clinically. although both pretreatment isolates were susceptible to ciprofloxacin, the posttreatment isolates were resistant to ciprofloxacin (mic = 32 micrograms/ml) and to other quinolones. the posttreatment isolates remained susceptible to nonquinolone antimicrobials. dna gyrase holoenzyme was ...19921313069
expression of helicobacter pylori urease genes in escherichia coli grown under nitrogen-limiting conditions.helicobacter pylori produces a potent urease that is believed to play a role in the pathogenesis of gastroduodenal diseases. four genes (urea, ureb, urec, and ured) were previously shown to be able to achieve a urease-positive phenotype when introduced into campylobacter jejuni, whereas escherichia coli cells harboring these genes did not express urease activity (a. labigne, v. cussac, and p. courcoux, j. bacteriol. 173:1920-1931, 1991). results that demonstrate that h. pylori urease genes could ...19921313413
profiles of toxin production by thermophilic campylobacter of animal origin.seventy-five strains of campylobacter jejuni and c. coli, which were isolated from a variety of animal species, primarily poultry, were examined for production of toxin. polymyxin extracts were tested in in vitro assays using cho-ki, fcl (foetal calf lung), vero, hela and cef (chicken embryo fibroblast) cells. the toxic effects observed were cell rounding and death. extracts from almost all c. jejuni and c. coli strains were toxic to both cho-ki and fcl cells and 69.0% of c. jejuni isolates and ...19921313623
in vitro activity of lomefloxacin and other antimicrobials against bacterial enteritis pathogens.lomefloxacin is a new, difluoroquinolone. in this study, the in vitro activity of lomefloxacin against clinical isolates of a variety of bacterial species associated with acute diarrheal disease was determined and compared with that of ciprofloxacin, ofloxacin, amoxicillin, sulphamethoxazole, trimethoprim, tetracycline, and chloramphenicol. bacterial isolates were obtained from different geographical areas, including western europe and the united kingdom, southern europe, africa, the middle east ...19921319301
cloning of campylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of c. jejuni by shuttle transposon mutagenesis.campylobacter jejuni is a gram-negative pathogen responsible for diarrhoeal diseases in humans. to date, very little is known about the genetic organization and molecular biology of this microorganism. the cosmid vector phc79 was used to construct a genomic library from the total genomic dna of c. jejuni strain c31 in escherichia coli and recombinant cosmids capable of complementing the auxotrophic defect in leucine biosynthesis of e. coli hb101 were identified. three of 400 clones tested were f ...19921322552
differentiation between thermophilic campylobacter species by species-specific antibodies.the four species of thermophilic campylobacters, campylobacter jejuni, c. coli, c. upsaliensis and c. lari, are difficult to distinguish from each other because of their lack of reactivity in many conventional biochemical and physiological tests. those tests which do discriminate sometimes give discordant results. species-specific antibody preparations (aps), capable of discriminating between the thermophilic campylobacter species by dot-elisa, were raised by inoculation of mice with partially p ...19921322880
[acute campylobacter jejuni enterocolitis resistant to quinolones in an hiv-positive patient]. 19921327169
in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ...19921332587
immunological cross-reactivity between outer membrane pore proteins of campylobacter jejuni and escherichia coli.immunocrossreactivity between the major outer membrane protein (momp) of campylobacter jejuni 85h and the ompc porin of escherichia coli k-12 was observed. these results indicate that a common antigenic domain is conserved in both momp and ompc. this antigenic region is detected only after a 96 degrees c treatment suggesting that it is buried in the native conformation of the respective porins. in addition, differences were observed between the major outer membrane proteins from various c. jejun ...19921337053
[preparation and use of fish fillet infusion as a basic medium for culturing bacteria].the authors present the first results on the utilization of fish infusion (ifp) as a basic medium for the cultivation of bacteria. the infusion was obtained from a common marine fish, corvina (micropogonias furnieri) according to the technique used in the preparation of beef infusion broth. streptococcus pyogenes, s. pneumoniae, neisseria meningitidis, campylobacter jejuni, escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus and bacillus su ...19921345299
physical map of campylobacter jejuni tgh9011 and localization of 10 genetic markers by use of pulsed-field gel electrophoresis.the physical map of campylobacter jejuni tgh9011 (atcc 43430) was constructed by mapping the three restriction enzyme sites sacii (ccgcgg), sali (gtcgac), and smai (cccggg) on the genome of c. jejuni by using pulsed-field gel electrophoresis and southern hybridization. a total of 25 restriction enzyme sites were mapped onto the c. jejuni chromosome. the size of the genome was reevaluated and was shown to be 1,812.5 kb. ten c. jejuni genetic markers that have been isolated in our laboratory were ...19921350583
serotype distribution of campylobacter jejuni and campylobacter coli isolated from hospitalized patients with diarrhea in central australia.campylobacter jejuni and/or campylobacter coli was cultured from 218 of 1,078 patients of all age groups admitted to alice springs hospital, alice springs, central australia, between july 1988 and june 1989 for treatment of diarrhea. one hundred sixty-six campylobacter colonies from 127 patients were subjected to o serotyping by using the penner typing scheme. all except 29 colonies could be serotyped. a total of 46 serotypes were identified, and the predominant serotypes were o:8, 17, o:22, o:1 ...19921370848
serological diversity and chemical structures of campylobacter jejuni low-molecular-weight lipopolysaccharides.low-mr lipopolysaccharides (lps) of campylobacter jejuni reference strains for serotypes o:1, o:4, o:23, and o:36 were examined through the liberation of core oligosaccharides by mild acid cleavage of the ketosidic linkage of 3-deoxy-d-manno-2-octulosonic acid residues to the lipid a moiety. the liberated oligosaccharides were examined for chemical structure by compositional analysis and methylated linkage analysis in conjunction with fast atom bombardment-mass spectrometry of permethylated olig ...19921370951
variation of the o antigen of campylobacter jejuni in vivo.during the course of a clinical study on patients with campylobacteriosis, three consecutive isolates of campylobacter jejuni from the same patient were sent for o-serotyping. marked differences in the specificities of the o antigens of the isolates were observed between the first and third isolates when a passive haemagglutination assay system developed for serotyping c. jejuni was used. differences in specificity were also demonstrated by immunoblots of lipopolysaccharides (lps) from proteinas ...19921372364
biochemical and antigenic properties of the campylobacter flagellar hook protein.the flagellar filament-hook complex was removed from campylobacter cells by shearing and was purified by differential solubilization and ultracentrifugation at ph 11 followed by cesium chloride buoyant density ultracentrifugation. flagellar filaments were then dissociated in 0.2 m glycine-hcl (ph 2.2), and purified hooks were collected by ultracentrifugation. the hooks (105 by 24 nm) each displayed a conical protrusion at the proximal end, a concave cavity at the distal end, and helically arrang ...19921375929
in vivo production of a stable single-stranded cdna in saccharomyces cerevisiae by means of a bacterial retron.gram-negative bacteria such as myxococcus xanthus, stigmatella aurantiaca, and escherichia coli contain retroelements called retrons. retrons consist of the msr-msd region and the gene for reverse transcriptase (rt), which are essential for the production of the branched rna-linked ms-dna (multicopy single-stranded dna). in this study, we attempted to produce msdna in the yeast saccharomyces cerevisiae. retron ec67 from e. coli, which is responsible for the production of msdna-ec67, was cloned u ...19921378616
ribosomal rna gene restriction fragment diversity amongst lior biotypes and penner serotypes of campylobacter jejuni and campylobacter coli.diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst 42 strains of campylobacter jejuni and 18 strains of c. coli including representatives of 53 different penner serotypes. haeiii ribopatterns were coded for numerical analysis which showed that all except two were different including those of several strains of the same serotype (p2 and p20). at the 30% similarity level, four groupings were formed in the analysis of which three corresponded to c. j ...19921381326
prevalence of antibodies against heat-stable antigens from helicobacter pylori in patients with dyspeptic symptoms and normal persons.heat-stable antigens from helicobacter pylori were investigated for the detection of serum igg, iga and igm antibodies against h. pylori by an elisa technique. antibody titers against h. pylori were measured in 167 dyspeptic patients, of whom 96 were h. pylori positive confirmed by culture or microscopy, and in 482 controls (0-98 years). increased igg antibody titers were found significantly more often in dyspeptic patients with active chronic gastritis than in patients with normal morphology, a ...19921389098
structures of the o chains from lipopolysaccharides of campylobacter jejuni serotypes o:23 and o:36.lipopolysaccharides of c. jejuni serotypes o:23 and o:36 have been shown to contain structurally variable o polysaccharide chains with repeating units of four closely-related types: ----3)-beta-d-glcpnac-(1----3)-alpha-d-galp-(1----2)-6d-alpha-d-alt-h epp-(1---- , ----3)-beta-d-glcpnac-(1----3)-alpha-d-galp-(1----2)-6d-3-me-alpha-d-alt -hepp- (1----, ----3)-beta-d-glcpnac-(1----3)-alpha-d-galp-(1----2)-d-glycero-alpha-d-a lt-hepp - (1----, and ----3)-beta-d-glcpnac-(1----3)-alpha-d-galp-(1----2) ...19921394309
mannose-resistant haemagglutination by campylobacter jejuni--a preliminary communication.ten strains of c. jejuni each isolated respectively from patients with diarrhoea and from chicken intestine (10 strains from each source) were examined for presumptive colonization factor(s) by measuring their cell surface hydrophobicity and haemagglutination. none of the strains expressed cell surface hydrophobicity. however, 14 strains (7 from either source) showed variable haemagglutination pattern with human, sheep and rabbit erythrocytes in the presence of 0.5 per cent d-mannose. thus, mann ...19921398807
lipid a in helicobacter lipid a of helicobacter pylori was characterized with regard to chemical composition, reactivity with anti-lipid a antibodies, and activity in a limulus lysate assay. the predominant fatty acids of h. pylori lipid a were 3-oh-18:0, 18:0, 3-oh-16:0, 16:0, and 14:0. hexosamine was present in amounts similar to those in campylobacter jejuni or salmonella typhimurium lipid a. the lipopolysaccharide of h. pylori contained 2-keto-3-deoxyoctonic acid, a common constituent of enterobacterial and c. ...19921398948
human immune response to campylobacter jejuni proteins expressed in vivo.campylobacter jejuni 81-176 grown in vivo in rabbit ileal loops expresses novel proteins that are not expressed under standard laboratory culture conditions. a new protein with a molecular mass of ca. 180 kda is expressed at 14, 24, and 48 h of infection. three other proteins, with molecular masses of ca. 66, 43, and 35 kda, are overexpressed during different phases of infection. expression of these proteins stops immediately during the first passage in laboratory media, and they do not elicit a ...19921399004
altered synthetic response of campylobacter jejuni to cocultivation with human epithelial cells is associated with enhanced internalization.campylobacter jejuni has been shown to bind to and enter epithelial cells in culture. the interaction of c. jejuni with int 407 epithelial cells was examined to determine whether bacterial protein synthesis is required for either binding or internalization. chloramphenicol, a selective inhibitor of bacterial protein synthesis, significantly reduced the internalization, but not binding, of c. jejuni compared with untreated controls as determined by protection from gentamicin. electrophoretic anal ...19921399005
specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...19921400961
[in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria].we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ...19921402063
[bacteremia from campylobacter jejuni in a cirrhotic patient with ascites]. 19921405812
alcohol dehydrogenase mediated acetaldehyde production by helicobacter pylori--a possible mechanism behind gastric injury.two standard helicobacter pylori strains showed significant cytosolic alcohol dehydrogenase activity and produced considerable amounts of acetaldehyde when incubated with an ethanol containing solution in vitro. the alcohol dehydrogenase activity of the helicobacter pylori strains was almost as high as that found in klebsiella pneumoniae and far greater than that in escherichia coli or campylobacter jejuni. the amount of acetaldehyde produced by cytosol prepared from helicobacter pylori exceeded ...19921406052
[chemotherapy of campylobacter infections].a total of 600 patients with suspected alimentary food poisoning were hospitalized. the ++clinico-laboratory findings showed that 27 (4.5 per cent) of them had campylobacter infection. the cultures of campylobacter jejuni and campylobacter coli were isolated from 24 and 3 patients, respectively. the patients underwent complex pathogenetic treatment with oral rehydration saline solutions, symptomatic agents, enzymatic preparations and diet (the basic therapy) supplemented with biological bacteria ...19921417342
competitive exclusion of campylobacters from poultry with k-bacteria and broilact.the competitive exclusion (ce) product (broilact) which is effective against salmonellas, was found to be inactive against campylobacters. microecological concepts were applied in the search of a new competitive flora and two novel strains ('k-bacteria') were isolated. these strains resembled campylobacters but differed from them in morphology, enzyme profiles (api), cellular fatty acid profiles and when tested with a ribosomal rna hybridization probe (gene-trak). two-week laboratory trials on b ...19921419531
simple and economical culture of campylobacter jejuni and campylobacter coli in co2 in moist air.strains of campylobacter jejuni and c. coli representing the 18 serogroups (lior) most commonly isolated from humans in canada were grown on solid media in an atmosphere of 10% co2 in moist air, 99% relative humidity. when the growth of all 18 serogroups on mueller hinton agar in a microaerobic atmosphere (5% o2, 10% co2 and 85% n2) was compared with the growth of all 18 serogroups on the same media in 10% co2 in moist air, colony sizes were significantly larger (p less than 0.05) for strains gr ...19921419543
a cytotonic, cholera toxin-like protein produced by campylobacter jejuni.1. campylobacter jejuni is a major cause of gastroenteric infection. 2. this organism appears to produce both cytotonic and cytotoxic virulence factors. 3. we report here that culture filtrates of some clinical isolates of c. jejuni induce elongation of chinese hamster ovary (cho) cells in vitro but do not cause inhibition of fluid absorption in the rat ileum. 4. these culture filtrates contain low levels of a protein which cross-reacts immunologically with the cholera toxin. 5. the cholera toxi ...19921424561
prevalence, biotypes, plasmid profile and antimicrobial resistance of campylobacter isolated from wild and domestic animals from northeast portugal.the incidence of campylobacter jejuni and campylobacter coli in wild and producing animals has been studied to evaluate their importance as potential reservoirs of campylobacter infection. these organisms were isolated from: 59 chicken (60.2%), 65 swine (59.1%), 31 black rats (57.4%), 61 sparrows (45.5%), 21 ducks (40.5%), 32 cows (19.5%) and 27 sheep (15.3%). biotypes, plasmid and resistance profiles were studied in order to characterize the isolates. biotypes i and ii of c. jejuni were predomi ...19921429305
[clinical studies on the treatment of campylobacter enteritis--emergence of quinolone-resistant campylobacter jejuni after treatment with new quinolones].in recent years, new quinolones such as ofloxacin (oflx) and tosufloxacin (tflx) have been frequently used in the treatment of bacterial enteritis caused by unknown organisms. the agent of first choice for the treatment of campylobacter enteritis is one of the macrolides, but new quinolones are often administered accidentally to adult patients with campylobacter enteritis. we have detected quinolone-resistant strains of campylobacter jejuni (c. jejuni) after the treatment of some patients with n ...19921431368
impact of zinc supplementation on intestinal permeability in bangladeshi children with acute diarrhoea and persistent diarrhoea syndrome.zinc has been shown to enhance intestinal mucosal repair in patients suffering from acrodermatitis enteropathica; but the impact on mucosal integrity during acute (ad) or persistent (pd) diarrhoea is unknown. one hundred eleven children with ad and 190 with pd aged between 3 and 24 months received, randomly and blind to the investigators, either an elemental zinc supplement of 5 mg/kg body wt/day or placebo in multivitamin syrup for 2 weeks while intestinal permeability and, biochemical and anth ...19921432467
differentiated caco-2 cells as a model for enteric invasion by campylobacter jejuni and c. coli.a collection of 44 campylobacter isolates (37 c. jejuni and seven c. coli) from children with colitis (21 strains) or watery diarrhoea (23 strains) was analysed for toxin production, association with hela cells, and invasion of differentiated caco-2 cell cultures. there was no obvious association of clinical symptoms with species, biotype or enterotoxin production. all colitis strains and most of the isolates from watery diarrhoea were cytotoxic for chinese hamster ovary cells. measurements of b ...19921433253
mononuclear cell response in the liver of mice infected with hepatotoxigenic campylobacter jejuni.intragastric inoculation with hepatotoxigenic strains of campylobacter jejuni led to the death of mice during the late phase of infection. histological study disclosed a massive infiltration of mononuclear cells in the liver, mimicking intrahepatic hypersensitivity. neither enterotoxigenic nor enteroinvasive escherichia coli induced such a lesion. however, the same histopathological change was induced by injecting the hepatotoxic factor of hepatotoxigenic c. jejuni intravenously on two occasions ...19921433254
campylobacter jejuni in notes of pregnancies with proven campylobacter jejuni infections were collected from 2 queensland teaching hospitals and reviewed. of these cases, 2 pregnancies (3 fetuses) resulted in stillbirths, 2 neonates required treatment with antibiotic therapy within 2 days of birth, while the remaining 2 pregnancies were treated at the time of the infection and were not associated with adverse outcome. maternal campylobacter infection should be actively sought for in the patient with suspected infe ...19921445138
uncommon campylobacter species in infant macaca nemestrina monkeys housed in a nursery.studies were conducted to characterize 18 isolates of campylobacter spp. that could not be identified as either campylobacter jejuni or c. coli. the isolates were cultured from specimens from 13 of 18 infant nonhuman primates during a prospective epidemiologic study reported previously. phenotypic tests, dna hybridization, and analysis of dna coding for rrna identified the isolates as c. butzleri (seven isolates), c. hyointestinalis (seven isolates), and c. fetus subsp. fetus or c. fetus subsp. ...19921452677
accuracy of the e test for determining antimicrobial susceptibilities of staphylococci, enterococci, campylobacter jejuni, and gram-negative bacteria resistant to antimicrobial agents.we compared the results of the e test mic method with the results of agar dilution susceptibility testing for 18 antimicrobial agents against 324 strains of gram-positive and gram-negative bacteria, including 99 strains of staphylococci, 101 strains of antimicrobial-resistant gram-negative bacteria, 40 strains of enterococci, and 84 isolates of campylobacter jejuni. overall agreement of mics (+/- 1 log2 dilution) was 97.3% for staphylococci, 94.6% for gram-negative bacilli, and 100.0% for entero ...19921452709
prevalence of bacterial agents of diarrhoeal disease at the national university hospital, singapore and their resistance to antimicrobial agents.over a 50-month period, 4,508 stool specimens from patients with diarrhoea were bacteriologically examined at the national university hospital, singapore. salmonella serotypes other than the typhoid and paratyphoid bacilli were the most common finding, being isolated in 10.8% of cases. campylobacter jejuni was unexpectedly infrequent (1.9%); aeromonas hydrophila was found in 1.8%. no other aerobic pathogen occurred in more than 1% of cases. clostridium difficile was sought only when requested, a ...19921455527
occurrence, removal and seasonal variation of "thermophilic" campylobacters in a sewage treatment plant in italy.monitoring of "thermophilic" campylobacters in a sewage treatment plant in bologna (italy) has shown that incoming sewage contained a most probable number of 1630 campylobacters/100 ml. the secondary treatment in activated sludge tanks reduced 98.61% of campylobacters, 95.32% of fecal coliforms, 96.46% of fecal streptococci, 93.36% of salmonellas and 93.01% and 88.29% of bod5 and cod respectively. subsequent tertiary treatment with 3 ppm of chlorine dioxide for 15 min reduced 100% of campylobact ...19921457031
acute pancreatitis associated with campylobacter jejuni bacteremia. 19921457641
anti-enterobacteria antibodies in psoriatic arthritis.the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ...19921458698
structural studies of peptidoglycans in campylobacter species.peptidoglycans (pg) from campylobacter coli, campylobacter jejuni, and campylobacter fetus were composed of muramic acid, glucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1.1:1:1.7:1.1:09. thirty percent of the amino groups of diaminopimelic acid were involved in cross-linkages between peptides. during cultivation, c. coli and c. jejuni changed from a spiral to a coccoid form. in c. coli, we could isolate pg only from the spiral forms in yields of 0.8-1.2% by dry ...19921461153
rapid emergence of quinolone resistance in campylobacter jejuni in patients treated with norfloxacin.15/60 subjects from one center, who all took part in a multicenter double-blind, placebo-controlled study to evaluate the effect of norfloxacin on acute enteritis, had norfloxacin sensitive strains of campylobacter jejuni in pre-study stool specimens. eight of the 15 subjects received active drug. in 3 of these 8, high-level quinolone resistant campylobacter strains of the same serotype as in pre-treatment samples were isolated 4-90 days after the initiation of treatment.19921465591
erysipelas-like skin lesions associated with campylobacter jejuni septicemia in patients with hypogammaglobulinemia.three cases are reported of hypogammaglobulinemic males with recurrent campylobacter jejuni septicemia and erysipelas-like cellulitis without diarrhoea. in one patient campylobacter jejuni grew from skin biopsy specimens. the findings in another patient were strongly suggestive of osteomyelitis caused by campylobacter jejuni. since the susceptibility of hypogammaglobulinemic patients to infection with campylobacter jejuni is probably related to a lack of serum bactericidal activity against campy ...19921468426
[campylobacter jejuni bacteremia in patient with acquired immunodeficiency syndrome and hepatic cirrhosis]. 19921469949
antibiotic susceptibility patterns and plasmid profile of helicobacter isolates from nigeria.antibiotic susceptibility testing and plasmid screening of 50 local isolates of campylobacter coli and campylobacter jejuni were done. all isolates were sensitive to ofloxacin but were resistant to ampicillin, cloxacillin, penicillin, streptomycin and aztreonam. a large number of isolates were sensitive to nalidixic acid (96%), ceftriaxone (96%) and chloramphenicol (86%). it is noteworthy that nine (18%) of the isolates were resistant to each of erythromycin, 15 (30%) were found to harbour plasm ...19921476963
antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli isolated in sweden: a 10-year follow-up report.resistance to erythromycin and doxycycline and more recently to fluoroquinolones has been reported to occur in campylobacter spp. both in vitro and in patients treated with these antibiotics. the frequency of resistance to 14 antimicrobial agents in campylobacter jejuni and campylobacter coli isolated from patients infected in sweden or abroad is described. for some agents, a comparison of susceptibility in strains of campylobacter spp. isolated in 1978 with those isolated in 1988 is made. no ge ...19921482158
degenerate pcr primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria.many bacterial responses to environmental stimuli are mediated by response regulators which coordinately regulate genes involved in particular adaptive responses. degenerate oligonucleotide primers were used to amplify by the polymerase chain reaction (pcr), fragments from genes encoding eleven novel response regulators. sequence and phylogenetic analysis revealed that phob, phop and creb gene fragments had been amplified from yersinia enterocolitica and yersinia pseudotuberculosis, and that a c ...19921490612
antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin.a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ...19921490908
serotype of campylobacter jejuni, hla, and the guillain-barré syndrome. 19921495517
isolation of nonchemotactic mutants of campylobacter jejuni and their colonization of the mouse intestinal tract.three nonchemotactic mutants (d54, y14, and n74) of campylobacter jejuni were isolated from wild-type strain fum158432 by either the negative swarming or liquid gradient method with brucella broth as the attractive substance. strains d54 and y14 were isolated after mutagenesis with methyl methanesulfonate, and n74 was isolated from a nonmutagenized culture. these mutants all failed to swarm on a semisolid medium and did not show any chemotactic behavior in the hard-agar plus assay method for any ...19921500167
iron acquisition and hemolysin production by campylobacter jejuni.campylobacter jejuni strains were tested for their ability to acquire iron from various iron sources present in humans. hemin, hemoglobin, hemin-hemopexin, and hemoglobin-haptoglobin stimulated the growth of c. jejuni strains in low-iron medium. transferrin, lactoferrin, and ferritin were unable to provide iron to the strains tested. derivatives of the naturally transformable c. jejuni strain 81-176 were isolated on the basis of their inability to use hemin as an iron source. these mutants were ...19921500194
campylobacter jejuni bacteraemia in children with diarrhoea in bangladesh: report of six cases.campylobacter jejuni was isolated from blood cultures from 6 of 6,275 diarrhoeal children seeking treatment at the clinical research centre of the international centre for diarrhoeal disease research, bangladesh (icddr,b) between april 1989 and december 1990. the clinical records of these 6 children were reviewed. all children were male; 5 were less than 1-year old and were severely malnourished. five patients presented with watery diarrhoea and one with bloody diarrhoea. two children died in th ...19921500639
characterization of two plasmids from campylobacter jejuni isolates that carry the apha-7 kanamycin resistance determinant.two small plasmids of 11.5 and 9.5 kb, each carrying an apha-7 kanamycin phosphotransferase gene, were studied. the mics of kanamycin for the two human campylobacter jejuni isolates harboring the plasmids were 10,000 and 5,000 micrograms/ml, while the mics of amikacin were 32 and 8 micrograms/ml, respectively. the mics of gentamicin and tobramycin were less than or equal to 2 micrograms/ml for both isolates. the restriction endonuclease maps of the plasmids were similar, with the larger plasmid ...19921503433
explosive campylobacter jejuni diarrhea in immunoproliferative small intestinal disease.campylobacter jejuni is an infrequent cause of self limiting acute diarrheal disease in adults in the indian subcontinent. we report the occurrence of a life threatening diarrhea due to c jejuni infection in a patient with immunoproliferative small intestinal disease. we postulate that immunosuppression due to malignancy, malnutrition and cancer chemotherapy was responsible for the unusually severe diarrhea.19921506053
a multiple logistic model for predicting the occurrence of campylobacter jejuni and campylobacter coli in water.a multiple logistic regression model was established to predict the occurrence of campylobacter jejuni/coli, related to index bacteria such as faecal coliforms, faecal streptococci, and sulphite-reducing clostridia, in a water source in southern norway. the fitted model indicated that faecal coliforms were strong predictors for c. jejuni/coli, although the water temperature also had a strong influence on results. sulphite-reducing clostridia, faecal streptococci, and season of the year had no si ...19921512182
[detection of campylobacter species by using polymerase chain reaction and nonradioactive labeled dna probe].we have detected campylobacter species which are now recognized as major pathogens of acute diarrheal disease in humans using polymerase chain reaction (pcr) and a nonradioactive labeled dna probe. diagnosis of campylobacter enteritis without doing culture from stool samples is of great benefit in the laboratory. two oligonucleotide primers (20 mer) complementary to a unique sequence of the dna encoding ribosomal rna (rrna) of campylobacter jejuni for pcr were synthesized by solid-phase phosphoa ...19921513035
campylobacter jejuni isolated from ratites. 19921515499
use of oligodeoxynucleotide probes to verify campylobacter jejuni as a cause of bovine abortion. 19921515500
[acute aortic insufficiency following endocarditis due to infection with campylobacter fetus subspecies fetus].a 50-year-old male alcoholic addict, examined because of diarrhoea with fever was found to have campylobacter jejuni in blood and stool cultures. after administration of broad-spectrum penicillin all acute symptoms disappeared but he lost 8 kg within 3 months and his general state health gradually deteriorated. after 3 months he suddenly developed leg oedema, dyspnoea and bouts of fever up to 38.8 degrees c. a loud cardiac murmur was now heard. echocardiography demonstrated vegetations on the re ...19921516529
campylobacter gastroenteritis in children in riyadh, saudi arabia.campylobacter jejuni/coli (cjc) was isolated from the stools of 82 (1 per cent) of 7369 children with gastrointestinal symptoms during a 2-year period. among 1130 control children, cj was isolated from the stool of one (0.09 per cent). the peak incidence of cjc associated gastroenteritis was in the winter. seventy-six per cent of the patients were 4 years of age and younger with the highest incidence (56 per cent) in children 2 years old and younger. the most common presenting symptoms and signs ...19921527809
role of campylobacter jejuni as a placental pathogen.members of the genus campylobacter are well recognised as enteric pathogens but have rarely been implicated as human placental pathogens. a case of septic abortion due to c jejuni is reported. this occurred in a previously healthy woman with a diarrhoeal illness. the presence of chorioamnionitis raises the possibility of ascending infection rather than septicaemic spread as the pathogenesis. members of the genus campylobacter have special culture requirements and their incidence as placental pat ...19921541701
campylobacter jejuni enteritis mistaken for ulcerative colitis.campylobacter jejuni is the most common bacterial cause of gastroenteritis and is often missed by routine stool cultures. patients may present with a clinical syndrome and endoscopic findings that are similar to acute ulcerative colitis. ciprofloxacin is currently the recommended antibiotic therapy.19921541961
[fever, headache, diarrhea].a 26-year-old man suddenly developed fever, headache, pain in the lower extremities, diarrhoea, and lymphadenopathy on the way back from thailand. subsequently, leukopenia, thrombocytopenia, coagulation abnormality and a morbilliform exanthema were noted. with symptomatic treatment the patient could be dismissed in good health after nine days. the diagnosis of dengue and campylobacter jejuni/coli infection could be verified serologically by a rising antibody titer and by a positive stool culture ...19921542765
campylobacter enteritis in saudi arabia.a 12-month survey on the incidence of campylobacter infection in 1217 patients with diarrhoea was carried out in jeddah, saudi arabia. campylobacters were isolated from 55 (4.5%) patients, second in prevalence to salmonellas (6.2%). shigellas were isolated from 4.2% of patients. campylobacter isolation rates were high in children of all ages, as well as in young adults (36.5% of all isolates were from adults aged 20-39 years). isolation rates peaked in september and november. analysis of the res ...19921547840
campylobacter species as a cause of diarrhoea in children in calcutta.from 1985 to 1988, 857 children (aged between 1 day and 60 months) admitted to hospital with diarrhoea and 241 controls (aged between 5 days and 60 months) were examined for campylobacters and other enteric pathogens by means of conventional methods. the difference between the isolation rates of campylobacters in those cases in which no other enteric pathogen was found (4.8%) and controls (6.2%) was not significant (p greater than 0.05). strains of campylobacter jejuni/coli were isolated through ...19921548418
in vitro binding of campylobacter jejuni/coli outer membrane preparations to int 407 cell membranes.outer membrane fractions (oms) of nine campylobacter (c.) jejuni and two c. coli strains belonging to different serovars, from human and various animal origins, were extracted by treatment with sodium n-lauryl sarcosinate. using n-octyl-beta-d-glucopyranoside a 42-kda protein and a flagella-enriched fraction were obtained. the capacity of the crude bacterial om preparations, the purified 42-kda protein and the flagella to bind to membranes of the human embryonic intestinal cell line int 407 was ...19921549070
the gene for the s7 ribosomal protein of chlamydia trachomatis: characterization within the chlamydial str operon.the prokaryotic ribosomal operon, str, contains open reading frames for the two elongation factors, elongation factor g (ef-g) and elongation factor tu (ef-tu), and ribosomal proteins s7 and s12. the dna sequence and predicted amino acid sequence for s7 from chlamydia trachomatis are presented and compared with homologues from other prokaryotes. also, the relationship of the s7 gene to the open reading frames for ribosomal protein s12 and ef-g is described. significant amino acid homology is als ...19921552847
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