| immune responses to soluble antigens of treponemes. | sonicates from five cultivable treponemes were used as antigens in delayed hypersensitivity tests and macrophage inhibition assays. immunodiffusion analysis showed that the sonicate comprised two major antigenic components which were not distinguishable in the skin tests. the sonicate antigens elicited significant cell-mediated immunity in guinea-pigs. treponema refringens biotype nichols proved to induce the strongest delayed response. delayed skin hypersensitivity to the antigens of treponema ... | 1977 | 402976 |
| arginine catabolism by treponema denticola. | treponema denticola, an anaerobe commonly present in the human mouth, ferments various amino acids and glucose. amino acid analyses indicated that substrate amounts of arginine were utilized by t. denticola growing in a complex, serum-containing medium. cell suspensions metabolized l-arginine to citrulline, nh3, co2, proline, and small amounts of ornithine. co2, nh3, ornithine, and proline were produced from l-citrulline by cell suspensions. determinations of radioactivity in products formed fro ... | 1976 | 977548 |
| comparison of the benzoyl-dl-arginine-naphthylamide (bana) test, dna probes, and immunological reagents for ability to detect anaerobic periodontal infections due to porphyromonas gingivalis, treponema denticola, and bacteroides forsythus. | most forms of periodontal disease are associated with the presence or overgrowth of anaerobic species that could include treponema denticola, porphyromonas gingivalis, and bacteroides forsythus among others. these three organisms are among the few cultivable plaque species that can hydrolyze the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). in turn, bana hydrolysis by the plaque can be associated with periodontal morbidity and with the presence of these three bana-positiv ... | 1992 | 1311335 |
| treponema denticola and porphyromonas gingivalis as prognostic markers following periodontal treatment. | subgingival plaque samples were collected from individuals with advanced periodontitis before and 3 to 11 weeks after scaling and root planing periodontal treatment. the plaque levels of treponema denticola and porphyromonas gingivalis antigens were measured before and after treatment by a quantitative immunoassay procedure using monoclonal antibodies specific for these oral bacteria. a decrease in mean levels of t. denticola (p less than .05) and p. gingivalis antigens (p less than .09) were ob ... | 1992 | 1315388 |
| inhibition of peptidase and glycosidase activities of porphyromonas gingivalis, bacteroides intermedius and treponema denticola by plant extracts. | aqueous extracts from 5 plants used widely in kenya as chewing sticks (mswaki) for the control of oral hygiene were tested for their ability to inhibit extracellular peptidase and glycosidase enzyme activities produced by the periodontopathic bacteria porphyromonas gingivalis (formerly bacteroides gingivalis), bacteroides intermedius and treponema denticola. the plants studied were rhus natalensis, cupressus hisitanica, sida cordifolia, olea africana and euclea divinorum. protease activities, in ... | 1992 | 1325483 |
| homology of a plasmid from the spirochete treponema denticola with the single-stranded dna plasmids. | the 2,647-bp nucleotide sequence of cryptic plasmid ptd1, isolated from the oral spirochete treponema denticola, was determined. the sequence revealed two open reading frames, a and b, which encode polypeptides of 335 and 235 amino acids, respectively. open reading frame a shows sequence similarity to genes that encode replication proteins from a group of plasmids common in gram-positive bacteria, which replicate via a single-stranded intermediate. | 1992 | 1339420 |
| identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. | activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ... | 1992 | 1398963 |
| polymerase chain reaction for detection of leptospira spp. in clinical samples. | a sensitive assay for leptospira spp., the causative agent of leptospirosis, was developed on the basis of the polymerase chain reaction (pcr). a 331-bp sequence from the leptospira interrogans serovar canicola rrs (16s) gene was amplified, and the pcr products were analyzed by dna-dna hybridization by using a 289-bp fragment internal to the amplified dna. specific pcr products also were obtained with dna from the closely related nonpathogenic leptospira biflexa but not with dna from other spiro ... | 1992 | 1400983 |
| growth of porphyromonas gingivalis, treponema denticola, t. pectinovorum, t. socranskii, and t. vincentii in a chemically defined medium. | a chemically defined medium, omiz (oral microbiology and immunology, zürich)-w1 was developed. medium omiz-w1 supports the long-term proliferation of a wide range of oral anaerobes, including representative strains of four treponema species and porphyromonas gingivalis. high concentrations of ascorbic acid and ammonium ions proved to be important for the growth of these organisms. t. denticola cd-1 grew in the absence of polyamines and long-chain fatty acids, t. pectinovorum and t. socranskii re ... | 1992 | 1400984 |
| cloning and expression of the aspartate carbamoyltransferase gene from treponema denticola. | treponema denticola seems to play a central role in the etiology of human periodontal disease. we have cloned an antigenic protein-coding sequence from t. denticola atcc 33520. the protein-coding region was found to be a 3-kbp hindiii-hindiii fragment. the open reading frame consists of 1,426 bp and codes for a protein with an m(r) of 54,919. the deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of escherichia coli. the gene products showed asparta ... | 1992 | 1444372 |
| comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? | the development of diagnostic tests for a periodontal infection raises the issue as to what the appropriate reference standard, or "gold standard," should be for the evaluation of a new test. the present research was initiated to compare the ability of several detection methods, i.e., a serial dilution anaerobic culture and/or microscopic procedure, a dna probe procedure, and immunological reagents using both an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay to detect ... | 1992 | 1537912 |
| hemagglutination activity of treponema denticola grown in serum-free medium in continuous culture. | hemagglutination by different treponema denticola strains was observed for erythrocytes of human, horse, bovine, and rabbit origin. the growth of t. denticola atcc 33520 in serum-free medium in continuous culture enabled us to study the hemagglutinating activity of freshly harvested spirochetes of a defined physiological status. the hemagglutinating activity was cell bound and not related to motility or appendages, such as fimbriae. the activity was destroyed by proteolytic enzymes, heat, and al ... | 1992 | 1563762 |
| characterization, cloning, and binding properties of the major 53-kilodalton treponema denticola surface antigen. | treponema denticola surface proteins were studied for their biochemical and biological characteristics. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis of detergent extracts of whole cells revealed a major protein of 53 kda and a number of minor proteins. antiserum raised against whole cells of t. denticola atcc 35405 reacted with the 53-kda protein and a 72-kda protein but not with the other proteins. immunoelectron microscopy with anti-53-kda-protein antibodies sh ... | 1992 | 1563796 |
| treponema denticola induces actin rearrangement and detachment of human gingival fibroblasts. | spirochetes are associated with destructive periodontal diseases, and one cultivatable oral species, treponema denticola, binds to mammalian cells and perturbs metabolism. to evaluate the cytoskeletal responses and attachment functions of human gingival fibroblasts (hgf) exposed to t. denticola, monolayers of hgf were incubated with t. denticola strains atcc 35405, e, and e' in serum-free medium. hgf retracted pseudopods, rounded up, and ultimately detached from the substratum. scanning electron ... | 1992 | 1639504 |
| specific immunofluorescence staining of treponema pallidum in smears and tissues. | to date, tissue sections prepared from formalin-fixed tissues have not been successfully stained with treponema pallidum subspecies-specific antibody in a direct fluorescent-antibody assay. while current methods stain t. pallidum, they do not distinguish t. pallidum from other spirochetes such as borrelia burgdorferi (e. f. hunter, p. w. greer, b. l. swisher, a. r. simons, c. e. farshy, j. a. crawford, and k. r. sulzer, arch. pathol. lab. med. 108:878-880, 1984). because trypsin pretreatment of ... | 1991 | 1709944 |
| biochemical properties of the outer membrane of treponema denticola. | the outer membranes (oms) from serovars a, b, and c of treponema denticola, originally isolated from periodontal patients, were prepared. dialysis of the oms against 20 mm mgcl2 yielded the aggregable (a) and the nonaggregable (na) moieties of the oms. the absence of muramic acid, adenosine triphosphatase, hexokinase, and nucleic acid as well as electron microscopy indicated that the om preparations were homogeneous. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the a and na moiet ... | 1991 | 1715883 |
| morphology and dynamics of protruding spirochete periplasmic flagella. | we recently characterized the three-dimensional shape of treponema phagedenis periplasmic flagella (pfs). in the course of these studies, we observed protrusions on swimming cells that resembled pfs. here we present a detailed characterization of the shape, structure, and motion of these protrusions. although protrusion formation occurred primarily in wild-type cells during the stationary phase, a large fraction of exponential-phase cells of cell cylinder helicity mutants (greater than 90% of mu ... | 1992 | 1732217 |
| characteristics of hemolytic and hemagglutinating activities of treponema denticola. | treponema denticola, a suspected periodontal pathogen, was shown to agglutinate and lyse human red blood cells. both activities were cell-associated, heat-labile, and produced during the exponential growth phase. hemolytic activity was time-dependent and required incubation at 37 degrees c; further incubation at 4 degrees c increased the hemolysis. hemagglutination was reduced in the presence of d-glucosamine, edta or sodium salicylate, whereas hemolytic activity was affected by calcium chloride ... | 1991 | 1812467 |
| modeling bacterial damage to pulpal cells in vitro. | there is increasing evidence that access to patent dentinal tubules by bacteria and their products rather than trauma from restorative materials is responsible for subsequent pulpitides. the purpose of this study was to compare the relative cytotoxicity of centrifugal fractions of two bacteria, fusobacterium nucleatum and treponema denticola, on l929 cells in monolayer cultures and in the "in vitro pulp chamber." neutrophilic chemotaxis assays and limmulus assays were performed to verify biologi ... | 1991 | 1895035 |
| phylogenetic analysis of the spirochetes. | the 16s rrna sequences were determined for species of spirochaeta, treponema, borrelia, leptospira, leptonema, and serpula, using a modified sanger method of direct rna sequencing. analysis of aligned 16s rrna sequences indicated that the spirochetes form a coherent taxon composed of six major clusters or groups. the first group, termed the treponemes, was divided into two subgroups. the first treponeme subgroup consisted of treponema pallidum, treponema phagedenis, treponema denticola, a thermo ... | 1991 | 1917844 |
| heat shock response of spirochetes. | we examined the heat shock response of the pathogenic spirochetes treponema pallidum, borrelia burgdorferi, and leptospira interrogans and certain saprophytic spirochetes. cellular proteins synthesized after shifts to higher temperatures were [35s]methionine labeled and analyzed by gel electrophoresis and fluorography. only t. pallidum failed to exhibit an obvious heat shock response. groel and dnak homologs were identified in the various species, although these proteins were not thermoinducible ... | 1991 | 2004832 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| characterization of monoclonal antibodies to two treponema denticola serotypes by the indirect fluorescent-antibody assay. | members of the genus treponema have been implicated as possible aetiologic agents of periodontal disease. previously developed murine monoclonal antibodies (mabs) to two treponema denticola strains were characterized by an indirect fluorescent-antibody (ifa) technique. fifteen t. denticola strains and 32 other bacteria commonly isolated from periodontal pockets were used in the screening process. two monoclonal antibodies to t. denticola atcc 33521, serotype b, reacted with five t. denticola iso ... | 1991 | 2030646 |
| molecular cloning and sequence analysis of antigen gene tdpa of treponema denticola. | we isolated and characterized an immunogenic protein of an oral spirochete, treponema denticola johnson. a genomic dna library constructed with bacteriophage lambda embl3 as a vector was immunologically screened with a rabbit antiserum against the whole cells. using western immunoblot analysis, we found a particular clone encoding an antigen with a molecular weight of 53,000; we designated the antigen as t. denticola protein a (tdpa). complete sequence determination revealed an open reading fram ... | 1991 | 2037356 |
| multivariate analyses of cellular fatty acids and carbohydrates of 1:2:1 and 2:4:2 spirochetes. | delimitation of small-sized spirochetes of the oral cavity can be difficult. the endoflagella pattern has long served as the main criterion for taxonomic distinction. for approved species, e.g. treponema denticola, several endoflagella patterns are observed, which indicates that this criterion is inadequate. the present study with gc and gc-ms used fatty acids and carbohydrates of whole-cell methanolysates to distinguish 1:2:1 and 2:4:2 subgingival spirochetes. thirteen fatty acids: c12:0, c13:0 ... | 1991 | 2054175 |
| activation of complement by treponema denticola. | oral spirochetes have been shown to be associated with periodontal diseases and are present in increased numbers in lesions of greater severity. in this study, the interaction of treponema denticola with human complement, a major antibacterial defense system, was examined. for each of two strains of t. denticola, it was found that both the classical and alternative pathways of human complement were activated in human serum upon incubation at 37 degrees c. c3 fragments were deposited on the surfa ... | 1991 | 1991867 |
| benzoyl-arginine naphthylamide (bana) hydrolysis by treponema denticola and/or bacteroides gingivalis in periodontal plaques. | treponema denticola and bacteroides gingivalis are among the few recognized species found in periodontal pockets that can hydrolyze the synthetic peptide n-benzoyl-dl-arginine-2-naphthylamide (bana). we determined the presence of these periodontal pathogens in bana-positive and -negative plaque samples through the use of indirect immunofluorescent antibody techniques. eighteen of 27 diseased sites gave bana-positive reactions, and 9 gave bana-negative reactions. t. denticola was present in 16 of ... | 1990 | 2098702 |
| use of monoclonal antibodies to enumerate spirochetes and identify treponema denticola in dental plaque of children, adolescents and young adults. | identification of spirochetes in dental plaque is difficult. not all spirochetes can be cultured and microscopic analysis based on darkfield or phase optics cannot determine the genus and species of individual bacterial cells. the purpose of this study was to use monoclonal antibodies in an immunoenzyme technique to stain spirochetes in dental plaque. separate mab were used to estimate total spirochetes and relative numbers of 2 distinct types of treponema denticola. plaque samples were collecte ... | 1991 | 1945493 |
| chemical and biological activities of a 64-kilodalton outer sheath protein from treponema denticola strains. | this study examined the distribution of the major outer sheath proteins (mosp) in several treponema denticola strains and reports the isolation of a 64-kda protein from the outer sheath of human clinical isolate t. denticola gm-1. the outer sheath was isolated by freeze-thaw procedures, and the distribution of outer sheath proteins was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). t. denticola gm-1, ms25, sr-5, and three low-passage clinical isolates possessed ... | 1991 | 1938897 |
| sulfhydryl-dependent attachment of treponema denticola to laminin and other proteins. | attachment of treponema denticola atcc 35405 to laminin, a major basement membrane protein, and to other proteins was studied. microdilution plates were coated with the proteins, and the attachment of t. denticola was measured by the enzyme-linked immunosorbent assay technique. compared with bovine serum albumin (bsa), t. denticola had a high affinity to laminin, fibronectin, fibrinogen, and gelatin, as well as to type i and type iv collagens. attachment to rgd peptide (gly-arg-gly-asp-ser, the ... | 1991 | 1937780 |
| [a rapid diagnosis for periodontitis based on the enzymatic activity of periodontopathic bacteria. the application of the sk-013 to the judgement on the effect of initial preparation]. | the purpose of the present study was to examine the diagnostic value of the sk-013 (sunstar, osaka) in evaluating the effect of initial preparation. the sk-013 is a highly sensitive reagent to measure peptidase activity specifically produced by bacteroides gingivalis. bacteroides forsythus, and treponema denticola. thirty-six sites in 16 periodontitis patients were monitored in this study. the clinical status of each site was assessed using the clinical indices such as gingival index (gi), plaqu ... | 1990 | 2133687 |
| pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis. | the purpose of this investigation was to determine whether monoclonal antibodies against pathogen-restricted antigens of treponema pallidum subsp. pallidum could be used as probes for spirochetes in diseased gingival tissue from subjects with acute necrotizing ulcerative gingivitis. a biotin-streptavidin system was used to identify spirochetes bound by monoclonal antibodies in cryostat sections of tissue. twelve of 16 tissue samples from diseased sites, but none of 8 tissue specimens from health ... | 1991 | 1855985 |
| inhibition of protease activities of periodontopathic bacteria by extracts of plants used in kenya as chewing sticks (mswaki). | extracts from five plants used as chewing sticks, and tannic acid, gallic acid and methyl ester of gallic acid, were tested for their ability to inhibit proteolytic activities of three strains of bacteroides gingivalis, three strains of bacteroides intermedius and two strains of treponema denticola. aqueous extract from the plants rhus natalensis and euclea divinorum were the most inhibitory of those tested, inhibiting by 50% the proteolytic activity of the test organisms, at concentrations of u ... | 1990 | 2142592 |
| cloning and expression of protease genes from treponema denticola in escherichia coli. | a gene bank of random fragments of chromosomal dna from treponema denticola atcc 33520 was constructed in the bacteriophage vector lambda l47.1. the gene bank was plated on escherichia coli c600 and screened for the presence of plaques in which enzyme activity was expressed, using overlays of fluorogenic synthetic substrates and a two-step procedure in which immunological screening was followed by enzyme assays of immunopositive lysates. recombinants expressing trypsin-like, chymotrypsin-like an ... | 1991 | 1840314 |
| [first results with a microbiological quick diagnostic procedure in periodontitis]. | we tested a new diagnostic procedure for the characterization of subgingival plaque in 119 subjects suffering from adult periodontitis. the method can be characterized as a hydrolysis of bana (n-benzoyl-dl-arginine-beta-naphthylamide) by peptidases of porphyromonas gingivalis, bacteroides forsythus and treponema denticola. the result of a positive will be a more or less blue coloured test paper representing the amount of these three peridontal pathogens into the subgingival plaque. after the per ... | 1991 | 1818626 |
| multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. | the association of bacteroides gingivalis, bacteroides forsythus, treponema denticola, and actinobacillus actinomycetemcomitans among others with periodontal disease offers the opportunity for the development of diagnostic tests that are based upon the detection and/or quantification of one or more of these organisms or their by-products in the plaque. three of the putative periodontal pathogens namely, t. denticola, b. gingivalis, and b. forsythus, can hydrolyze the synthetic trypsin substrate, ... | 1990 | 2181111 |
| use of randomly cloned dna fragments for the identification of oral spirochetes. | dna probes were produced for the detection and identification of 4 cultivable species of oral spirochetes, treponema denticola, treponema socranskii, treponema vincentii and treponema pectinovorum. to obtain probe sequences, chromosomal dna, isolated from representative strains within each species, was cloned in escherichia coli k-12. cloned dna fragments were screened for the ability to hybridize to dna only from homologous strains. several such fragments were identified and shown to be specifi ... | 1991 | 1682869 |
| qualitative assessment of treponema denticola and bacteroides gingivalis in human subgingival plaque. | | 1991 | 1668639 |
| the benzoylarginine peptidase from treponema denticola (strain aslm), a human oral spirochaete: evidence for active-site carboxyl groups. | the benzoylarginine peptidase of treponema denticola (strain aslm; a human oral spirochaete) was progressively and irreversibly inactivated by 1-(ethoxycarbonyl)-2-ethoxy-1, 2-dihydroquinoline, a carboxyl-group reagent. at acidic ph values, reaction of one mole of the modifier per active site of the enzyme resulted in total inactivation of the enzyme. assuming that this modifier is a specific carboxyl reagent, the data suggest that the inactivation of the t. denticola benzoylarginine peptidase w ... | 1990 | 2280691 |
| growth stimulation of treponema denticola by periodontal microorganisms. | previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ... | 1990 | 2321929 |
| cross-reactivity of nonspecific treponemal antibody in serologic tests for lyme disease. | serum samples obtained from 59 persons who had acute necrotizing ulcerative gingivitis, periodontitis, syphilis, or lyme disease were tested against treponema phagedenis biotype reiter, treponema denticola, treponema vincentii, and treponema scoliodontum by indirect fluorescent-antibody staining methods. although there were positive reactions for sera representing each of these study groups and for 20 (13%) of 156 samples collected from the general population (premarital screening for syphilis), ... | 1990 | 2380356 |
| the collagenolytic activity of treponema denticola. | | 1992 | 1480016 |
| extrachromosomal elements of spirochetes. | the spirochetes include some important pathogenic bacteria, treponema, borrelia and leptospira. the pathogeneses of these spirochetes are very diverse. in an attempt to learn more about the virulence factors among the spirochetes, their genetic organization and capacity have been studied. structural analysis of the genome in borrelia has shown that the genome is composed of one linear maxi-chromosome with additional linear minichromosomes as well as several supercoiled circular plasmids. moreove ... | 1992 | 1475522 |
| cell-bound peptidase activities of treponema denticola atcc 33520 in continuous culture. | the oral spirochaete treponema denticola atcc 33520 was grown at a mean generation time of 10 h in anaerobic continuous culture in a serum- and carbohydrate-free medium at ph 7.0. the extracellular proteolytic activities of this spirochaete were then investigated by incubating washed cells with 68 2-naphthylamide derivatives of the extended api system. chymotrypsin-like, trypsin-like, elastase-like and iminopeptidase activities were demonstrated. the phenylalanine peptidase or chymotrypsin-like ... | 1992 | 1402787 |
| relative proportions of pathogen-related oral spirochetes (pros) and treponema denticola in supragingival and subgingival plaque from patients with periodontitis. | the purpose of this study was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen-related oral spirochete (pros) and specific serovars of treponema denticola. plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. individual monoclonal antibodies were used to determine whether spirochetes were present an ... | 1992 | 1372653 |
| monoclonal antibodies that recognize a specific surface antigen of treponema denticola. | spirochetes have been implicated as potential etiologic agents of periodontitis in humans. murine monoclonal antibodies (mabs) specific for a serogroup of treponema denticola, an oral spirochete, were developed and characterized in this study. antibodies secreted by clone iaa11 were judged to be the most useful, since they were able to detect 8 of 15 t. denticola strains. this mab consisted of an immunoglobulin g3 heavy chain and a kappa light chain. mab iaa11 was found to react with an epitope ... | 1988 | 2447020 |
| [chemiluminescence response and phagocytic activity of murine polymorphonuclear leukocytes to various species of oral bacteria]. | polymorphonuclear leukocytes (pmn) are predominant cells in the gingival crevice and saliva, and may play an important role in oral bacteria. murine peritoneal pmn was used and stimulated with 9 genera, 17 species of oral bacteria, including cariogenic and periodontal pathogens. the pmn response to the bacteria was measured by the luminol mediated chemiluminescence (cl) response and phagocytic activities, and the activities of lysozyme in the reaction medium after the cl response were also measu ... | 1989 | 2489336 |
| in vitro susceptibilities of oral bacterial isolates to spiramycin. | four hundred strains of oral bacteria were tested for their susceptibility to spiramycin. actinobacillus actinomycetemcomitans and most species of lactobacillus were resistant to the antibiotic. all strains of cariogenic streptococcus mutans and most strains of bacterial species implicated in adult chronic periodontitis (bacteroides gingivalis, b. intermedius, and treponema denticola) were susceptible to spiramycin. | 1989 | 2610512 |
| antigenic and structural analysis of treponema denticola. | polypeptide and western immunoblot profiles of subcellular fractions of treponema denticola atcc 33520 have been determined by sds-page of triton x-100-soluble and -insoluble fractions, a lipopolysaccharide-enriched fraction and purified flagella. major triton x-100-soluble polypeptides of 72, 68, 54 and 52 kda were detected. the 54 kda polypeptide appeared to be a breakdown product of a larger, heat-modifiable polypeptide. based on the results of sds-page analysis and immunoblotting of proteina ... | 1989 | 2636257 |
| demonstration of a bimodal coaggregation reaction between porphyromonas gingivalis and treponema denticola. | this study demonstrates a strong coaggregation reaction between 2 suspected periodontopathogens: porphyromonas gingivalis and treponema denticola. other black-pigmented oral bacterial species tested did not coaggregate with t. denticola. this specific interbacterial aggregation was bimodal, since heating of both cell types was required to completely eliminate the reaction. the coaggregation reaction occurred between ph 4 and 9. under some conditions, arginine and d-galactosamine were effective i ... | 1992 | 1337373 |
| clinical, microbiological and immunological studies of post-juvenile periodontitis. | the present study includes the clinical, microbiological and immunological examinations of 2 patients with post-juvenile periodontitis. bacteroides intermedius was the predominant isolate from periodontal pockets with post-localized juvenile periodontitis. bacteroides gingivalis, bacteroides forsythus and actinobacillus actinomycetemcomitans were detected in samples from periodontal pockets with post-generalized juvenile periodontitis. igg antibody levels to b. gingivalis were significantly high ... | 1989 | 2640920 |
| nutritional interactions between two suspected periodontopathogens, treponema denticola and porphyromonas gingivalis. | a mutual symbiotic enhancement of growth of porphyromonas gingivalis and treponema denticola is described in this report. brain heart infusion broth supplemented with vitamin k did not support the individual growth of p. gingivalis or t. denticola. however, when inoculated as a mixture, both bacterial species did grow significantly. the growth-stimulating factors produced by p. gingivalis and t. denticola were dialyzable and heat stable and were further identified as isobutyric acid and succinic ... | 1992 | 1333450 |
| [microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy]. | the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ... | 1989 | 2700361 |
| bacterial synergy of treponema denticola and porphyromonas gingivalis in a multinational population. | treponema denticola and porphyromonas gingivalis have been associated with human adult severe periodontitis. in this study, we quantified these putative pathogens in subgingival plaque samples collected from 74 fijians, 74 colombians and 73 u.s. americans stationed at the multinational force and observers encampment in the sinai desert, egypt. a contingency table of t. denticola and p. gingivalis frequency revealed a highly significant synergistic relationship. we discovered that the occurrence ... | 1992 | 1326739 |
| a major antigen on the outer envelope of a human oral spirochete, treponema denticola. | human oral spirochetes are prominent inhabitants of subgingival plaque in patients with periodontal disease. by immunoelectron microscopy using protein a-gold complexes and either polyclonal mouse antiserum against the 53-kda antigen or 53-kda-antigen-specific monoclonal antibody, a major polypeptide antigen, with a molecular weight of 53,000 (molecular size, 53 kilodaltons [kda]), of a human oral spirochete, treponema denticola atcc 33520, was found to localize on the surface of the outer envel ... | 1989 | 2744855 |
| purification and substrate specificity of an endopeptidase from the human oral spirochete treponema denticola atcc 35405, active on furylacryloyl-leu-gly-pro-ala and bradykinin. | an endopeptidase was purified to homogeneity from the cell extracts of treponema denticola atcc 35405 (a human oral spirochete) by a procedure that comprised dialysis, anion exchange fast protein liquid chromatography (fplc), hydroxylapatite fplc, immobilized metal affinity fplc, fplc chromatofocusing, and two consecutive gel permeation fplc steps. the enzyme is a 62-kda protein with an isoelectric point of 6.5-7.0. experiments with enzyme inhibitors suggest that this enzyme is a metallopeptidas ... | 1992 | 1321141 |
| a sensitive enzymatic method (sk-013) for detection of treponema denticola, porphyromonas gingivals and bacteroides forsythus in subgingival plaque samples. | an enzymatic method, sk-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. this method was found to have specificity for porphyromonas gingivalis, treponema denticola, bacteroides forsythus, and some capnocytophaga strains. the purpose of this study was to determine whether sk-013 could indicate the presence of periodontopathic bacteria, including t. denticola, p. gingivalis and b. forsythus, which produce trypsin-like enzymes. subgingival plaque samp ... | 1992 | 1312592 |
| a sensitive enzymatic method (sk-013) for detection and quantification of specific periodontopathogens. | porphyromonas gingivalis, bacteroides forsythus, and treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. these microorganisms hydrolyze the synthetic peptide n-benzoyl-dl-arginine-2-naphthylamide (bana). in this study, we developed an enzymatic method, designated sk-013, to detect the existence of these microorganisms in subgingival plaque bacteria. this enzymatic method was based on the observation of the hydrolysis of n-carbobenzoxy-g ... | 1992 | 1312591 |
| comparative study of six random oral spirochete isolates. serological heterogeneity of treponema denticola. | | 1985 | 2935615 |
| molecular cloning of treponema pallidum outer envelope fibronectin binding proteins, p1 and p2. | phages directing the synthesis of treponema pallidum fibronectin binding adhesin proteins, p1 and p2, were isolated from an embl-3 bacteriophage lambda library of t pallidum deoxyribonucleic acid (dna). the recombinant phages were identified using antisera generated to treponemal proteins purified in fibronectin-sepharose. recombinant p1 and p2 proteins possessed the same relative molecular weights as the native surface polypeptides of spirochaetes. the structural genes for these proteins were s ... | 1987 | 2962928 |
| immunity in syphilis. studies in active immunity. | support for the concept of the development of immunity during the course of syphilis is avaiable in the literature. in experimental syphilis in rabbits, some immunity is present approximately 3 weeks after infection with treponema pallidum. resistance to re-infection increases to a maximum at approximately 3 months after infection. termination of this state by penicillin treatment within this 3-month period may enable re-infection to be accomplished. attempts to reproduce this state of immunity ... | 1976 | 791454 |
| [isolation and identification of oral spirochaetes and fusobacterium in juvenile periodontitis]. | periodontal pocket specimens of 40 juveniles periodontitis (jp) and specimens from gingival sulcus in 40 normal juveniles (np) were examined. 25 strains of anaerobic oral spirochaete (62.5%) (17 strains were treponema microdentium, 3 strains macrodentium, 2 strains t orale, 3 strains borrelia) and 21 strains of fusobacterium were isolated in 40 jp. in 40 np, 2 strains of spirochaetes (5%) and 3 strains fusobacterium (7.5%) were found. this study shows that there is a relationship between the inf ... | 1990 | 2098228 |
| a study of the acid phosphatase of treponema denticola. | this study describes some of the properties of the acid phosphatase of the potential periodontopathogen treponema denticola. the highest enzyme activity was found in 87 h old cells. two optimum phs for enzyme activity were detected, one at ph 4.8 and another at ph 6.2. divalent cations did not influence the acid phosphatase of t. denticola. the anion f- added in the form of naf and at a level greater than 20 micrograms/ml f- diminished the activity of the acid phosphatase of intact cells of t. d ... | 1990 | 2085369 |
| the formation of hydrogen sulfide and methyl mercaptan by oral bacteria. | the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ... | 1990 | 2082242 |
| detection of two anaerobic periodontopathogens in children by means of the bana and elisa assays. | the mouths of young children become colonized by a variety of bacteria, but there have been only a few studies that have sought the presence of periodontopathic species in this population. almost all of these studies used culturing techniques rather than the newer detection methodologies for various periodontopathogens. studies in adults have shown that treponema denticola and porphyromonas (bacteroides) gingivalis can be detected in dental plaque by use of the bana and elisa diagnostic tests. i ... | 1991 | 2066486 |
| susceptibility of fibronectin to degradation by various gram-negative and gram-positive oral micro-organisms. | the degradative effects on tritiated human plasma fibronectin (fn) of proteases associated with twenty five gram-negative (gn) and fifteen gram-positive (gp) oral microbial isolates were examined. ninety-two per cent of the gn and 20% of the gp isolates degraded the fn. in the gn group, the degradative effects were greatest for bacteroides gingivalis and treponema denticola, while in the gp group streptococcus faecalis produced appreciable, and the two lactobacillus casei isolates moderate and l ... | 1988 | 3287100 |
| comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes. | spirochetes are usually the predominant organisms observed microscopically in subgingival plaques removed from tooth sites associated with periodontitis, but these organisms are rarely isolated by cultural means, presumably because the media do not support their growth and/or because these fragile organisms are disrupted by the various procedures used to disperse plaque samples. in the present investigation, three dispersal procedures, sonification, mechanical mixing, and homogenization, were co ... | 1987 | 3320092 |
| isolation and characterization of a plasmid from treponema denticola. | agarose gel electrophoresis of whole genomic dna of the oral spirochaete treponema denticola has revealed a plasmid-like fraction. purification and restriction enzyme analysis has confirmed the presence of a 2.6-kb circular plasmid, which has been mapped for restriction sites and cloned into the escherichia coli plasmid puc18. southern blot analysis of genomic t. denticola dna, using the plasmid as a probe, has shown that the plasmid is present only as an extra-chromosomal element. no plasmid-co ... | 1991 | 2040428 |
| [a rapid diagnosis (sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. study on the characterization of the sk-013 and the enzyme specificity]. | we present studies for development of an enzymatic diagnostic method for periodontitis. a rapid and sensitive diagnostic method named sk-013 was provided by sunstar inc. to evaluate the peptidase activities specifically derived from periodontopathic bacteria such as bacteroides gingivalis, bacteroides forsythus and treponema denticola and some strains of capnocytophaga species. the results obtained indicated the specificity of the enzymatic reaction in this system. | 1990 | 2133684 |
| [a rapid diagnosis (sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. the relationship between the enzymatic activity (sk-013) and b. gingivalis, b. forsythus, t. denticola in subgingival microflora]. | sk-013 was developed for rapid detection of the peptidase activity in subgingival plaque samples. the purpose of this study was to determine whether sk-013 could be a marker for the presence of periodontopathic bacteria including treponema denticola, bacteroides gingivalis and bacteroides forsythus which produce trypsin-like enzyme. subgingival plaque samples were taken with 3 paper points from 10 clinically healthy sites and 30 periodontal diseased sites. the sk-013 activity of plaque sample wa ... | 1990 | 2133685 |
| serospecificity of a cloned protease-resistant treponema pallidum--specific antigen expressed in escherichia coli. | we evaluated the serological reactivity of a protease-resistant antigen designated 4d which was encoded by treponema pallidum dna and was expressed in escherichia coli from recombinant plasmid paw329. this 19,000-molecular-weight antigen was purified in its native, non-protease-treated form from e. coli sonic extracts by molecular sieving and ion-exchange chromatography. antibody binding to antigen 4d was detected by a radioimmunoassay. antigen 4d-specific antibody was detected in 95% of the ser ... | 1986 | 3514656 |
| [a rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms]. | there have been various laboratory methods for the microbiological diagnosis of periodontal disease. however, there have been some disadvantages in these methods. in this study of the application of a chair-side test for microbiological diagnosis, the activity of peptidases in periodontal pockets of patients was examined by using the assay system sk-013. sk-013 consists of synthetic substrates and is capable of rapidly (in 15 min) evaluating the activity of specific peptidase from treponema dent ... | 1990 | 2133686 |
| microscopic agglutination and polyacrylamide gel electrophoresis analyses of oral anaerobic spirochetes. | microscopic agglutination (ma) analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) were used to determine strain and species similarities and dissimilarities among three species of oral anaerobic spirochetes, treponema denticola, treponema pectinovorum, and treponema vincentii. the ma analysis revealed a diversity of serologic reactivity or sharing of common antigens within each species. however, there was no cross-reactivity or sharing of common antigens among the ... | 1986 | 3745424 |
| [chemical composition and biological activities of lipopolysaccharides extracted from treponema denticola and treponema vincentii]. | lipopolysaccharides (lpss) were isolated from treponema denticola (t. denticola) and treponema vincentii (t. vincentii) by the phenol/water (pw) and the phenol/chloroform/petroleum-ether (pcp) procedures. 1) t. denticola pw-lps (lps isolated by the pw procedure), pcp-sup-lps (lps isolated by the pcp procedure in the supernates of ultracentrifugation), pcp-ppt-lps (precipitated lps isolated by the pcp procedure, obtained after ultracentrifugation), and t. vincentii pcp-ppt-lps were composed of ca ... | 1990 | 2134963 |
| interaction of treponema denticola td-4, gm-1, and ms25 with human gingival fibroblasts. | the adherence of treponema denticola gm-1, td-4, and ms25 to human gingival fibroblasts (hgfs) was studied to serve as an introduction to investigations into the interactions of these oral bacteria with human host cells. under both aerobic (5% co2) and anaerobic (85% n2 plus 10% h2 plus 5% co2) environments, the interactions with the hgfs were such that strains gm-1 and ms25 were consistently more adherent than strain td-4. polyclonal antibodies to gm-1 inhibited gm-1 adherence by 70%, while ms2 ... | 1990 | 2160430 |
| isolation and characterization of the treponema denticola prta gene coding for chymotrypsinlike protease activity and detection of a closely linked gene encoding pz-plgpa-hydrolyzing activity. | the chymotrypsinlike protease gene (prta) from treponema denticola atcc 35405 was isolated from a lambda gt11 clone bank as one of several clones expressing protease activity. the dna from one positive clone capable of hydrolyzing type iv collagen was subcloned into plasmid vector puc119 for further analysis. deletion analysis of subclone pxq27.2 revealed the approximate location of the prta gene on the dna insert. deae-sephadex chromatography of crude cell extracts of the subclone revealed two ... | 1990 | 2174832 |
| ultrastructural studies of treponemes: location of axial filaments and some dimensions of treponema pallidum (nichols strain), treponema denticola, and treponema reiteri. | ultrathin sections of treponema pallidum (nichols strain), t. denticola (microdentium), and t. reiteri have been studied in the electron microscope to determine the location of the axial filaments and some of the dimensions of these organisms. the axial filaments of t. pallidum (nichols strain) have been seen to be tubular in cross section with an overall diameter of 21.0 +/- 0.73 nm, and an electron-lucent core of 8.0 nm. the filaments were found to lie on the outside of the organism which had ... | 1973 | 4570277 |
| amino acid and glucose fermentation by treponema denticola. | | 1971 | 4938689 |
| [studies on effects of treponema microdentium on cultured cells. i. effects of tissue culture medium 199 and eagle's minimum essential medium on the growth and survival of treponema microdentium]. | | 1965 | 5218829 |
| [electron microscopical studies on ultrathin sections of treponema microdentium]. | | 1966 | 5222883 |
| the utility of the bana test for monitoring anaerobic infections due to spirochetes (treponema denticola) in periodontal disease. | treponema denticola, porphyromonas gingivalis, and bacteroides forsythus each possesses an enzyme(s) that hydrolyzes the synthetic substrate benzoyl-dl-arginine-naphthylamide (bana). the presence of these organisms in a subgingival plaque sample can be determined by the ability of the plaque to hydrolyze bana. in the present study, we describe the usefulness of the bana test at various stages of a clinical trial of the efficacy of metronidazole in the treatment of periodontal disease. a bana-pos ... | 1990 | 2212216 |
| tip-oriented adherence of treponema denticola to fibronectin. | the adherence of treponema denticola to ligands on cell surfaces or in basement membranes of periodontal tissues might play an important role in its pathogenicity. a direct microscopic assay was used to examine the binding of t. denticola to fibronectin and other protein substrates adsorbed on plastic cover slips. all strains of t. denticola that were tested adhered to fibronectin but to different degrees. the strains which bound in high numbers frequently bound by their tips. type strain atcc 3 ... | 1990 | 2254019 |
| quantitative immunoassay of treponema denticola serovar c in adult periodontitis. | murine monoclonal antibodies specific for treponema denticola serovar c were produced and characterized in this study. an immunoassay was then developed by using these monoclonal antibodies, and the t. denticola serovar c antigen content of subgingival plaque was quantitated for samples taken from patients with periodontitis and healthy volunteers. the human subgingival plaque samples were grouped by severity of disease and pocket depth measurements at the collection site. the t. denticola serov ... | 1990 | 2380374 |
| development of a diagnostic test for anaerobic periodontal infections based on plaque hydrolysis of benzoyl-dl-arginine-naphthylamide. | treponema denticola, porphyromonas (bacteroides) gingivalis, and bacteroides forsythus are among the anaerobic species frequently associated with adult forms of periodontal disease. these organisms hydrolyze the synthetic peptide benzoyl-dl-arginine-naphthylamide (bana), and such enzyme activity can be detected in the plaque and related to clinical disease and the presence of spirochetes. in this investigation, the liquid bana assay was compared with a commercially developed bana assay which emp ... | 1990 | 2380379 |
| cellular location of a treponema denticola chymotrypsinlike protease and importance of the protease in migration through the basement membrane. | a number of immunological methods were used to localize a cell-associated treponema denticola chymotrypsinlike protease. indirect immunofluorescence staining, immunogold labeling, and an enzyme-linked immunosorbent assay all indicated that the protease was attached to the outside of the cell envelope. the invasive capability of t. denticola was evaluated by following the degradation of a reconstituted basement membrane material (matrigel) and the release of spirochetes from the gel. under condit ... | 1990 | 2404867 |
| keratinolytic activity of cutaneous and oral bacteria. | a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ... | 1987 | 2434427 |
| [nutritional requirements for oral treponema]. | oral treponema is still difficult to cultivate in vitro because of lacking of our knowledge about what they require for growth. the aim of this study was to find what were the nutritional requirements for them. treponema denticola, t. vincentii, and t. socranskii were cultivated in various broths to seek which fractions of tryptone and rabbit serum, or serum proteins were stimulatory. treponemal growth was assessed by turbidity readings. it was obvious that a weakly negative-charged peptide isol ... | 1989 | 2637426 |
| outer membrane ultrastructure explains the limited antigenicity of virulent treponema pallidum. | freeze fracture and deep etching were used to investigate the ultrastructural basis for the observation that anti-treponemal antibodies bind poorly to the surface of virulent treponema pallidum. fractures of t. pallidum outer membranes contained scarce, uniformly sized intramembranous particles (imps). imps on the convex faces often appeared to form linear arrays that wound in spirals about the organism. in contrast to the outer membrane, imps of the cytoplasmic membrane were randomly distribute ... | 1989 | 2648388 |
| [correlation between subgingival microflora and peptidase activity in periodontal pockets measured with synthetic substrates]. | the activity of peptidases in periodontal pockets of patients were examined by using four different synthetic enzyme substrates, and surveyed their correlation with the microbial populations of subgingival plaque and clinical symptoms. the substrates were (1) alpha-n-benzoyl-dl-arginine-beta-naphthylamide (bana), (2) n-carbobenzoxy-glycylglycyl-arginine-4-methoxy-beta-naphthylamide, (3) alpha-n-benzoyl-l-arginylglycyl-l-phenylalanyl-l-proline-4-methoxy -beta-naphthylamide and (4) n-carbobenzoxy- ... | 1989 | 2700363 |
| a common antigen of treponema denticola and other treponema species detected by monoclonal antibody. | murine monoclonal antibodies against treponema denticola were produced. one monoclonal antibody (msa257) reacted with 34,000 dalton antigens of all t. denticola strains, including atcc strains and our isolates. this monoclonal antibody also reacted with antigens of other treponema strains tested. | 1989 | 2762014 |
| adherence of treponema denticola to modified hydroxyapatite. | small samples of hydroxyapatite (ha) beads were coated with 10 microl of either saliva, serum, human crevicular fluid, or a preparation of lysosomal enzymes from human polymorphonuclear leucocytes (pmn), before being added to suspensions of treponema denticola. the beads were then observed with the scanning electron microscope. abundant t. denticola were found to adhere to ha or ha coated with saliva, serum, or crevicular fluid. coating the beads with lysosomal enzymes consistently prevented the ... | 1987 | 2824585 |
| ceruloplasmin can substitute for rabbit serum in stimulating the growth of treponema denticola. | the growth of treponema denticola in a complex medium was stimulated by human ceruloplasmin and, to a lesser extent, albumin, alpha-1-antitrypsin, alpha-1-acid glycoprotein, and alpha-2-macroglobulin. human ceruloplasmin could stimulate growth of t. denticola as well as or better than 5% rabbit serum, with a dose-response relationship of between 1 and 5 mg of ceruloplasmin per ml of culture medium. these results suggest that ceruloplasmin could substitute for rabbit serum in stimulating the grow ... | 1989 | 2912901 |
| isolation of a chymotrypsinlike enzyme from treponema denticola. | a chymotrypsinlike protease with an mr of 95,000 was extracted from treponema denticola atcc 35405 and was partially purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the proteolytic activity was detected in an electrophoretogram containing polyacrylamide that was conjugated to bovine serum albumin. a single band of activity was detected when the t. denticola extract was solubilized and electrophoresed in the presence of sodium dodecyl sulfate. no activity was fo ... | 1988 | 3166451 |
| immunologic profile of juvenile periodontitis. i. lymphocyte blastogenesis and the autologous mixed lymphocyte response. | studies of blastogenesis of lymphocytes in culture from juvenile periodontitis (jp) patients have been inconclusive. experiments demonstrating differences in lymphocyte blastogenesis to preparations of putative periodontopathogens in jp and phytohaemagglutinin (pha) were performed. variables in the blastogenesis assay system recently reported were controlled which included using a range of cell and activator concentrations, incubation times of 3, 5 and 7 days, conical-bottomed microtest wells an ... | 1984 | 6592318 |
| enzyme profiles of oral spirochetes in rapid-ana system. | enzyme profiles of oral treponema species were determined by using rapid-ana (innovative diagnostic system, atlanta, ga.), a 4-h test system which detects 18 enzymatic reactions, including aminopeptidases and glycosidases. seventy-two clinical isolates of treponema denticola, four reference strains of t. denticola (atcc 35404, atcc 35405, atcc 35520, and atcc 33521), one strain of t. vincentii (atcc 35580), and two strains of t. socranskii subspecies (t. socranskii subsp. buccale atcc 35534 and ... | 1988 | 3183013 |
| suppression of human lymphocyte responses by oral spirochetes: a monocyte-dependent phenomenon. | spirochetes have been implicated in the pathogenesis of several human infections including syphilis, yaws, lyme disease, and periodontal diseases. we examined soluble sonic extracts of oral spirochetes (treponema denticola and t. vincentii) for their ability to alter human lymphocyte function. these organisms were isolated from subgingival plaque of patients with periodontitis. we found that sonicates of several but not all strains of t. denticola caused a dose-dependent inhibition of human lymp ... | 1984 | 6699406 |
| locomotory characteristics of treponema denticola. | locomotion of pathogenic spirochetes has been suggested as a virulence factor in their pathogenesis. little is known of the locomotory characteristics of oral anaerobic spirochetes. we have determined the optimal conditions for motility of seven strains of treponema denticola in menstrua of different viscosities. the viscosity for optimum motility for all strains was found to be 9.57 centipoises at 25 degrees c. under these conditions the average speeds for each strain was computed from the moti ... | 1988 | 3203255 |
| api zym system for identification of bacteroides spp., capnocytophaga spp., and spirochetes of oral origin. | a total of 80 oral strains of bacteroides gingivalis, b. asaccharolyticus, b. melaninogenicus subsp. intermedius, b. melaninogenicus subsp. melaninogenicus, capnocytophaga, treponema denticola, and t. vincentii were characterized with the api zym system for 19 enzyme activities. comparison of anaerobic and aerobic incubation with nine reference strains of these organisms showed no important differences. the key differential tests for black-pigmented bacteroides strains and treponemes of oral ori ... | 1982 | 6764781 |
| quantitative relationship of treponema denticola to severity of periodontal disease. | the treponema denticola content of plaque was quantitatively estimated for samples taken from periodontitis patients as well as periodontally healthy subjects among two separate human populations. the populations studied included military volunteers and civilians at a university dental clinic. the plaque samples from each population were grouped according to pocket depth measurements at the collection site. a biotin-avidin enzyme-linked immunosorbent assay procedure was developed with a monoclon ... | 1988 | 3346072 |
| serum iga and igg antibodies to treponema vincentii and treponema denticola in adult periodontitis, juvenile periodontitis and periodontally healthy subjects. | 13 patients with untreated adult periodontitis (ap) were compared to 8 subjects free of periodontal disease (h) with respect to plaque index (pli), gingival index (gi), probing depth (pd) and differential counts of subgingival bacterial morphotypes from a pooled sample of 6 surfaces with the greatest probing depth. serum antibody levels to t. vincentii and t. denticola strains were also determined in these subjects as well as in the sera from 5 subjects with localized juvenile periodontitis (ljp ... | 1986 | 3464620 |