| phylogenetic analysis of the spirochetes. | the 16s rrna sequences were determined for species of spirochaeta, treponema, borrelia, leptospira, leptonema, and serpula, using a modified sanger method of direct rna sequencing. analysis of aligned 16s rrna sequences indicated that the spirochetes form a coherent taxon composed of six major clusters or groups. the first group, termed the treponemes, was divided into two subgroups. the first treponeme subgroup consisted of treponema pallidum, treponema phagedenis, treponema denticola, a thermo ... | 1991 | 1917844 |
| description of treponema azotonutricium sp. nov. and treponema primitia sp. nov., the first spirochetes isolated from termite guts. | long after their original discovery, termite gut spirochetes were recently isolated in pure culture for the first time. they revealed metabolic capabilities hitherto unknown in the spirochaetes division of the bacteria, i.e., h(2) plus co(2) acetogenesis (j. r. leadbetter, t. m. schmidt, j. r. graber, and j. a. breznak, science 283:686-689, 1999) and dinitrogen fixation (t. g. lilburn, k. s. kim, n. e. ostrom, k. r. byzek, j. r. leadbetter, and j. a. breznak, science 292:2495-2498, 2001). howeve ... | 2004 | 15006748 |
| complete genome sequence of treponema pallidum strain dal-1. | treponema pallidum strain dal-1 is a human uncultivable pathogen causing the sexually transmitted disease syphilis. strain dal-1 was isolated from the amniotic fluid of a pregnant woman in the secondary stage of syphilis. here we describe the 1,139,971 bp long genome of t. pallidum strain dal-1 which was sequenced using two independent sequencing methods (454 pyrosequencing and illumina). in rabbits, strain dal-1 replicated better than the t. pallidum strain nichols. the comparison of the comple ... | 2012 | 23449808 |
| genome sequence of the thermophilic fresh-water bacterium spirochaeta caldaria type strain (h1(t)), reclassification of spirochaeta caldaria, spirochaeta stenostrepta, and spirochaeta zuelzerae in the genus treponema as treponema caldaria comb. nov., treponema stenostrepta comb. nov., and treponema zuelzerae comb. nov., and emendation of the genus treponema. | spirochaeta caldaria pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bacterium that is motile via periplasmic flagella. the type strain, h1(t), was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in oregon, usa, and is of interest because it enhances the degradation of cellulose when grown in co-culture with clostridium thermocellum. here we provide a taxonomic re-evaluation for s. caldaria based on phylogenetic analyses of 16s rrna seque ... | 2013 | 23961314 |
| targeting the treponemal microbiome of digital dermatitis infections by high-resolution phylogenetic analyses and comparison with fluorescent in situ hybridization. | modern pyrosequencing technology allows for a more comprehensive approach than traditional sanger sequencing for elucidating the etiology of bovine digital dermatitis. we sought to describe the composition and diversity of treponemes in digital dermatitis lesions by using deep sequencing of the v3 and v4 hypervariable regions of the 16s rrna gene coupled with species-level taxonomic identification. treponema-specific 16s rrna gene pcrs and pyrosequencing were performed on biopsy specimens origin ... | 2013 | 23658264 |