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phylogenetic analysis of the spirochetes.the 16s rrna sequences were determined for species of spirochaeta, treponema, borrelia, leptospira, leptonema, and serpula, using a modified sanger method of direct rna sequencing. analysis of aligned 16s rrna sequences indicated that the spirochetes form a coherent taxon composed of six major clusters or groups. the first group, termed the treponemes, was divided into two subgroups. the first treponeme subgroup consisted of treponema pallidum, treponema phagedenis, treponema denticola, a thermo ...19911917844
how stable is stable? function versus community composition.the microbial community dynamics of a functionally stable, well-mixed, methanogenic reactor fed with glucose were analyzed over a 605-day period. the reactor maintained constant ph and chemical oxygen demand removal during this period. thirty-six rrn clones from each of seven sampling events were analyzed by amplified ribosomal dna restriction analysis (ardra) for the bacteria and archaea domains and by sequence analysis of dominant members of the community. operational taxonomic units (otus), d ...199910427068
flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose.methanogenic bioreactor communities were used as model ecosystems to evaluate the relationship between functional stability and community structure. replicated methanogenic bioreactor communities with two different community structures were established. the effect of a substrate loading shock on population dynamics in each microbial community was examined by using morphological analysis, small-subunit (ssu) rrna oligonucleotide probes, amplified ribosomal dna (rdna) restriction analysis (ardra), ...200010966429
complete genome sequence of the termite hindgut bacterium spirochaeta coccoides type strain (spn1(t)), reclassification in the genus sphaerochaeta as sphaerochaeta coccoides comb. nov. and emendations of the family spirochaetaceae and the genus sphaerochaeta.spirochaeta coccoides dröge et al. 2006 is a member of the genus spirochaeta ehrenberg 1835, one of the oldest named genera within the bacteria. s. coccoides is an obligately anaerobic, gram-negative, non-motile, spherical bacterium that was isolated from the hindgut contents of the termite neotermes castaneus. the species is of interest because it may play an important role in the digestion of breakdown products from cellulose and hemicellulose in the termite gut. here we provide a taxonomic re ...201222768363
the canine oral microbiome.determining the bacterial composition of the canine oral microbiome is of interest for two primary reasons. first, while the human oral microbiome has been well studied using molecular techniques, the oral microbiomes of other mammals have not been studied in equal depth using culture independent methods. this study allows a comparison of the number of bacterial taxa, based on 16s rrna-gene sequence comparison, shared between humans and dogs, two divergent mammalian species. second, canine oral ...201222558330
biology of moderately halophilic aerobic bacteria.the moderately halophilic heterotrophic aerobic bacteria form a diverse group of microorganisms. the property of halophilism is widespread within the bacterial domain. bacterial halophiles are abundant in environments such as salt lakes, saline soils, and salted food products. most species keep their intracellular ionic concentrations at low levels while synthesizing or accumulating organic solutes to provide osmotic equilibrium of the cytoplasm with the surrounding medium. complex mechanisms of ...19989618450
coexistence of bacterial sulfide oxidizers, sulfate reducers, and spirochetes in a gutless worm (oligochaeta) from the peru margin.olavius crassitunicatus is a small symbiont-bearing worm that occurs at high abundance in oxygen-deficient sediments in the east pacific ocean. using comparative 16s rrna sequence analysis and fluorescence in situ hybridization, we examined the diversity and phylogeny of bacterial symbionts in two geographically distant o. crassitunicatus populations (separated by 385 km) on the peru margin (water depth, approximately 300 m). five distinct bacterial phylotypes co-occurred in all specimens from b ...200515746360
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