| the soluble methane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph methylocystis sp. strain m. | in methanotrophic bacteria, methane is oxidized to methanol by the enzyme methane monooxygenase (mmo). the soluble mmo enzyme complex from methylocystis sp. strain m also oxidizes a wide range of aliphatic and aromatic compounds, including trichloroethylene. in this study, heterologous dna probes from the type ii methanotroph methylosinus trichosporium ob3b were used to isolate souble mmo (smmo) genes from the type ii methanotroph methylocystis sp. strain m. smmo genes from strain m are clustere ... | 1997 | 9143121 |
| assessment of changes in microbial community structure during operation of an ammonia biofilter with molecular tools. | biofiltration has been used for two decades to remove odors and various volatile organic and inorganic compounds in contaminated off-gas streams. although biofiltration is widely practiced, there have been few studies of the bacteria responsible for the removal of air contaminants in biofilters. in this study, molecular techniques were used to identify bacteria in a laboratory-scale ammonia biofilter. both 16s rrna and ammonia monooxygenase (amoa) genes were used to characterize the heterotrophi ... | 1998 | 9835577 |
| cytochrome p460 genes from the methanotroph methylococcus capsulatus bath. | p460 cytochromes catalyze the oxidation of hydroxylamine to nitrite. they have been isolated from the ammonia-oxidizing bacterium nitrosomonas europaea (r. h. erickson and a. b. hooper, biochim. biophys. acta 275:231-244, 1972) and the methane-oxidizing bacterium methylococcus capsulatus bath (j. a. zahn et al., j. bacteriol. 176:5879-5887, 1994). a degenerate oligonucleotide probe was synthesized based on the n-terminal amino acid sequence of cytochrome p460 and used to identify a dna fragment ... | 1998 | 9851984 |
| high-molecular-mass multi-c-heme cytochromes from methylococcus capsulatus bath. | the polypeptide and structural gene for a high-molecular-mass c-type cytochrome, cytochrome c553o, was isolated from the methanotroph methylococcus capsulatus bath. cytochrome c553o is a homodimer with a subunit molecular mass of 124,350 da and an isoelectric point of 6. 0. the heme c concentration was estimated to be 8.2 +/- 0.4 mol of heme c per subunit. the electron paramagnetic resonance spectrum showed the presence of multiple low spin, s = 1/2, hemes. a degenerate oligonucleotide probe syn ... | 1999 | 9922265 |
| halomethane:bisulfide/halide ion methyltransferase, an unusual corrinoid enzyme of environmental significance isolated from an aerobic methylotroph using chloromethane as the sole carbon source. | a novel dehalogenating/transhalogenating enzyme, halomethane:bisulfide/halide ion methyltransferase, has been isolated from the facultatively methylotrophic bacterium strain cc495, which uses chloromethane (ch(3)cl) as the sole carbon source. purification of the enzyme to homogeneity was achieved in high yield by anion-exchange chromatography and gel filtration. the methyltransferase was composed of a 67-kda protein with a corrinoid-bound cobalt atom. the purified enzyme was inactive but was act ... | 1999 | 10508052 |
| methanotroph diversity in landfill soil: isolation of novel type i and type ii methanotrophs whose presence was suggested by culture-independent 16s ribosomal dna analysis. | the diversity of the methanotrophic community in mildly acidic landfill cover soil was assessed by three methods: two culture-independent molecular approaches and a traditional culture-based approach. for the first of the molecular studies, two primer pairs specific for the 16s rrna gene of validly published type i (including the former type x) and type ii methanotrophs were identified and tested. these primers were used to amplify directly extracted soil dna, and the products were used to const ... | 1999 | 10543800 |
| molecular characterization of functional and phylogenetic genes from natural populations of methanotrophs in lake sediments. | the 16s rrna and pmoa genes from natural populations of methane-oxidizing bacteria (methanotrophs) were pcr amplified from total community dna extracted from lake washington sediments obtained from the area where peak methane oxidation occurred. clone libraries were constructed for each of the genes, and approximately 200 clones from each library were analyzed by using restriction fragment length polymorphism (rflp) and the tetrameric restriction enzymes mspi, haeiii, and hhai. the pcr products ... | 1999 | 10543824 |
| polyclonal antibodies recognizing the amob protein of ammonia oxidizers of the beta-subclass of the class proteobacteria. | a 41-kda protein of nitrosomonas eutropha was purified, and the n-terminal amino acid sequence was found to be nearly identical with the sequence of amob, a subunit of ammonia monooxygenase. this protein was used to develop polyclonal antibodies, which were highly specific for the detection of the four genera of ammonia oxidizers of the beta-subclass of proteobacteria (nitrosomonas, including nitrosococcus mobilis, which belongs phylogenetically to nitrosomonas; nitrosospira; nitrosolobus; and n ... | 2001 | 11133435 |
| detection of methanotroph diversity on roots of submerged rice plants by molecular retrieval of pmoa, mmox, mxaf, and 16s rrna and ribosomal dna, including pmoa-based terminal restriction fragment length polymorphism profiling. | the diversity of methanotrophic bacteria associated with roots of submerged rice plants was assessed using cultivation-independent techniques. the research focused mainly on the retrieval of pmoa, which encodes the alpha subunit of the particulate methane monooxygenase. a novel methanotroph-specific community-profiling method was established using the terminal restriction fragment length polymorphism (t-rflp) technique. the t-rflp profiles clearly revealed a more complex root-associated methanot ... | 2001 | 11526021 |
| quantitative detection of methanotrophs in soil by novel pmoa-targeted real-time pcr assays. | methane oxidation in soils is mostly accomplished by methanotrophic bacteria. little is known about the abundance of methanotrophs in soils, since quantification by cultivation and microscopic techniques is cumbersome. comparison of 16s ribosomal dna and pmoa (alpha subunit of the particulate methane monooxygenase) phylogenetic trees showed good correlation and revealed five distinct groups of methanotrophs within the alpha and gamma subclasses of proteobacteria: the methylococcus group, the met ... | 2003 | 12732507 |
| differences in hyporheic-zone microbial community structure along a heavy-metal contamination gradient. | the hyporheic zone of a river is nonphotic, has steep chemical and redox gradients, and has a heterotrophic food web based on the consumption of organic carbon entrained from downwelling surface water or from upwelling groundwater. the microbial communities in the hyporheic zone are an important component of these heterotrophic food webs and perform essential functions in lotic ecosystems. using a suite of methods (denaturing gradient gel electrophoresis, 16s rrna phylogeny, phospholipid fatty a ... | 2003 | 12957946 |
| wide distribution of a novel pmoa-like gene copy among type ii methanotrophs, and its expression in methylocystis strain sc2. | experiments were conducted to determine if a novel pmoa-like gene (pmoa2) recently discovered in the methane-oxidizing bacterium methylocystis strain sc2 (p. f. dunfield, m. tchawa yimga, s. d. dedysh, u. berger, w. liesack, and j. heyer, fems microbiol. ecol. 41:17-26, 2002) is present in other methane-oxidizing bacteria (mob), and if it is expressed. a newly developed primer combination (pmoa206f-pmoa703b) allowed a differential detection of pmoa1 and pmoa2. by using this primer combination, w ... | 2003 | 12957949 |
| impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene. | with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ... | 2005 | 15626648 |
| impacts of co-solvent flushing on microbial populations capable of degrading trichloroethylene. | with increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. this study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (tce)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a ... | 2005 | 15626648 |
| methanotrophic bacteria and facilitated transport of pollutants in aquifer material. | in situ stimulation of methanotrophic bacteria has been considered as a methodology for aquifer remediation. chlorinated aliphatic hydrocarbons such as trichloroethylene are fortuitously oxidized by the methane monooxygenase produced by methanotrophic bacteria. experimental results are presented that indicate that both colloidal suspensions containing methanotrophic cells and the soluble extracellular polymers produced by methanotrophic cells have the potential to enhance the transport and remov ... | 1994 | 16349401 |
| methanol promotes atmospheric methane oxidation by methanotrophic cultures and soils. | two methanotrophic bacteria, methylobacter albus bg8 and methylosinus trichosporium ob3b, oxidized atmospheric methane during batch growth on methanol. methane consumption was rapidly and substantially diminished (95% over 9 days) when washed cell suspensions were incubated without methanol in the presence of atmospheric methane (1.7 ppm). methanotrophic activity was stimulated after methanol (10 mm) but not methane (1,000 ppm) addition. m. albus bg8 grown in continuous culture for 80 days with ... | 1998 | 16349514 |
| characterization of root-associated methanotrophs from three freshwater macrophytes: pontederia cordata, sparganium eurycarpum, and sagittaria latifolia. | root-associated methanotrophic bacteria were enriched from three common aquatic macrophytes: pontederia cordata, sparganium eurycarpum, and sagittaria latifolia. at least seven distinct taxa belonging to groups i and ii were identified and presumptively assigned to the genera methylosinus, methylocystis, methylomonas, and methylococcus. four of these strains appeared to be novel on the basis of partial 16s ribosomal dna sequence analysis. the root-methanotroph association did not appear to be hi ... | 1998 | 16349515 |
| methane and trichloroethylene degradation by methylosinus trichosporium ob3b expressing particulate methane monooxygenase. | whole-cell assays of methane and trichloroethylene (tce) consumption have been performed on methylosinus trichosporium ob3b expressing particulate methane monooxygenase (pmmo). from these assays it is apparent that varying the growth concentration of copper causes a change in the kinetics of methane and tce degradation. for m. trichosporium ob3b, increasing the copper growth concentration from 2.5 to 20 mum caused the maximal degradation rate of methane (v(max)) to decrease from 300 to 82 nmol o ... | 1998 | 16349516 |
| differential effects of nitrogenous fertilizers on methane-consuming microbes in rice field and forest soils. | the impact of environmental perturbation (e.g., nitrogenous fertilizers) on the dynamics of methane fluxes from soils and wetland systems is poorly understood. results of fertilizer studies are often contradictory, even within similar ecosystems. in the present study the hypothesis of whether these contradictory results may be explained by the composition of the methane-consuming microbial community and hence whether methanotrophic diversity affects methane fluxes was investigated. to this end, ... | 2006 | 16461686 |
| planktonic and sediment-associated aerobic methanotrophs in two seep systems along the north american margin. | methane vents are of significant geochemical and ecological importance. notable progress has been made toward understanding anaerobic methane oxidation in marine sediments; however, the diversity and distribution of aerobic methanotrophs in the water column are poorly characterized. both environments play an essential role in regulating methane release from the oceans to the atmosphere. in this study, the diversity of particulate methane monooxygenase (pmoa) and 16s rrna genes from two methane v ... | 2008 | 18487407 |
| genome sequence of the methanotrophic alphaproteobacterium methylocystis sp. strain rockwell (atcc 49242). | methylocystis sp. strain rockwell (atcc 49242) is an aerobic methane-oxidizing alphaproteobacterium isolated from an aquifer in southern california. unlike most methanotrophs in the methylocystaceae family, this strain has a single pmo operon encoding particulate methane monooxygenase but no evidence of the genes encoding soluble methane monooxygenase. this is the first reported genome sequence of a member of the methylocystis species of the methylocystaceae family in the order rhizobiales. | 2011 | 21441518 |
| poly-3-hydroxybutyrate metabolism in the type ii methanotroph methylocystis parvus obbp. | differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (phb). previous researchers have demonstrated that phb functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how c(1) metabolism affects the role of phb. in the present study, the type ii methanotroph methylocystis parvus obbp did not replicate using s ... | 2011 | 21724874 |
| Chemistry and Biology of the Copper Chelator Methanobactin. | Methanotrophic bacteria, organisms that oxidize methane, produce a small copper chelating molecule called methanobactin (Mb). Mb binds Cu(I) with high affinity and is hypothesized to mediate copper acquisition from the environment. Recent advances in Mb characterization include revision of the chemical structure of Mb from Methylosinus trichosporium OB3b and further investigation of its biophysical properties. In addition, Mb production by several other methanotroph strains has been investigated ... | 2011 | 22126187 |
| Chemistry and Biology of the Copper Chelator Methanobactin. | Methanotrophic bacteria, organisms that oxidize methane, produce a small copper chelating molecule called methanobactin (Mb). Mb binds Cu(I) with high affinity and is hypothesized to mediate copper acquisition from the environment. Recent advances in Mb characterization include revision of the chemical structure of Mb from Methylosinus trichosporium OB3b and further investigation of its biophysical properties. In addition, Mb production by several other methanotroph strains has been investigated ... | 2011 | 22126187 |
| comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. | comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16s rrna gene clone library and improved cultivation-based techniques. our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses. | 2005 | 15812052 |
| radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils. | microorganisms that oxidize atmospheric methane in soils were characterized by radioactive labelling with (14)ch(4) followed by analysis of radiolabelled phospholipid ester-linked fatty acids ((14)c-plfas). the radioactive fingerprinting technique was used to compare active methanotrophs in soil samples from greenland, denmark, the united states, and brazil. the (14)c-plfa fingerprints indicated that closely related methanotrophic bacteria were responsible for the oxidation of atmospheric methan ... | 1999 | 10473417 |
| characterization of methanotrophic bacterial populations in soils showing atmospheric methane uptake. | the global methane cycle includes both terrestrial and atmospheric processes and may contribute to feedback regulation of the climate. most oxic soils are a net sink for methane, and these soils consume approximately 20 to 60 tg of methane per year. the soil sink for atmospheric methane is microbially mediated and sensitive to disturbance. a decrease in the capacity of this sink may have contributed to the approximately 1%. year(-1) increase in the atmospheric methane level in this century. the ... | 1999 | 10427012 |
| comparison of pmoa pcr primer sets as tools for investigating methanotroph diversity in three danish soils. | three particulate methane monooxygenase pcr primer sets (a189-a682, a189-a650, and a189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. each pcr primer set was used to construct a library containing 50 clones from each soil type. the clones from each library were grouped by restriction fragment length polymorphism, and representatives f ... | 2001 | 11525970 |
| mrna-based parallel detection of active methanotroph populations by use of a diagnostic microarray. | a method was developed for the mrna-based application of microbial diagnostic microarrays to detect active microbial populations. dna- and mrna-based analyses of environmental samples were compared and confirmed via quantitative pcr. results indicated that mrna-based microarray analyses may provide additional information on the composition and functioning of microbial communities. | 2006 | 16461725 |
| genomic and physiological analysis of carbon storage in the verrucomicrobial methanotroph "ca. methylacidiphilum fumariolicum" solv. | "candidatus methylacidiphilum fumariolicum" solv is a verrucomicrobial methanotroph that can grow in extremely acidic environments at high temperature. strain solv fixes carbon dioxide (co(2)) via the calvin-benson-bassham cycle with methane as energy source, a trait so far very unusual in methanotrophs. in this study, the ability of "ca. m. fumariolicum" to store carbon was explored by genome analysis, physiological studies, and electron microscopy. when cell cultures were depleted for nitrogen ... | 2012 | 23060867 |
| the transcriptome of bathymodiolus azoricus gill reveals expression of genes from endosymbionts and free-living deep-sea bacteria. | deep-sea environments are largely unexplored habitats where a surprising number of species may be found in large communities, thriving regardless of the darkness, extreme cold, and high pressure. their unique geochemical features result in reducing environments rich in methane and sulfides, sustaining complex chemosynthetic ecosystems that represent one of the most surprising findings in oceans in the last 40 years. the deep-sea lucky strike hydrothermal vent field, located in the mid atlantic r ... | 2012 | 23015773 |
| detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing. | genomic analysis of the methanotrophic verrucomicrobium "methylacidiphilum infernorum" strain v4 has shown that most pathways conferring its methanotrophic lifestyle are similar to those found in proteobacterial methanotrophs. however, due to the large sequence divergence of its methane monooxygenase-encoding genes (pmo), "universal" pmoa polymerase chain reaction (pcr) primers do not target these bacteria. unlike proteobacterial methanotrophs, "methylacidiphilum" fixes carbon autotrophically, a ... | 2012 | 22912630 |
| survival or revival: long-term preservation induces a reversible viable but non-culturable state in methane-oxidizing bacteria. | knowledge on long-term preservation of micro-organisms is limited and research in the field is scarce despite its importance for microbial biodiversity and biotechnological innovation. preservation of fastidious organisms such as methane-oxidizing bacteria (mob) has proven difficult. most mob do not survive lyophilization and only some can be cryopreserved successfully for short periods. a large-scale study was designed for a diverse set of mob applying fifteen cryopreservation or lyophilization ... | 2012 | 22539945 |
| diversity of cultivable methane-oxidizing bacteria in microsites of a rice paddy field: investigation by cultivation method and fluorescence in situ hybridization (fish). | the diversity of cultivable methane-oxidizing bacteria (mob) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (fish) techniques. seven microsites of a japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. based on pmoa gene analysis and transmission electron microscopy (tem), four type i, and nine type ... | 2012 | 22446309 |
| microbial minorities modulate methane consumption through niche partitioning. | microbes catalyze all major geochemical cycles on earth. however, the role of microbial traits and community composition in biogeochemical cycles is still poorly understood mainly due to the inability to assess the community members that are actually performing biogeochemical conversions in complex environmental samples. here we applied a polyphasic approach to assess the role of microbial community composition in modulating methane emission from a riparian floodplain. we show that the dynamics ... | 2013 | 23788331 |
| genome mining for methanobactins. | methanobactins (mbns) are a family of copper-binding natural products involved in copper uptake by methanotrophic bacteria. the few mbns that have been structurally characterized feature copper coordination by two nitrogen-containing heterocycles next to thioamide groups embedded in a peptidic backbone of varying composition. mbns are proposed to derive from post-translational modification of ribosomally synthesized peptides, but only a few genes encoding potential precursor peptides have been i ... | 2013 | 23442874 |
| the methanol dehydrogenase gene, mxaf, as a functional and phylogenetic marker for proteobacterial methanotrophs in natural environments. | the mxaf gene, coding for the large (α) subunit of methanol dehydrogenase, is highly conserved among distantly related methylotrophic species in the alpha-, beta- and gammaproteobacteria. it is ubiquitous in methanotrophs, in contrast to other methanotroph-specific genes such as the pmoa and mmox genes, which are absent in some methanotrophic proteobacterial genera. this study examined the potential for using the mxaf gene as a functional and phylogenetic marker for methanotrophs. mxaf and 16s r ... | 2013 | 23451130 |
| formate oxidation-driven calcium carbonate precipitation by methylocystis parvus obbp. | microbially induced carbonate precipitation (micp) applied in the construction industry poses several disadvantages such asammonia release to the air and nitric acid production. an alternative micp from calcium formate by methylocystis parvus obbp is presented here to overcome these disadvantages. to induce calcium carbonate precipitation, m. parvus was incubated at different calcium formate concentrations and starting culture densities. up to 91.4% ± 1.6% of the initial calcium was precipitated ... | 2014 | 24837386 |
| metaproteomic identification of diazotrophic methanotrophs and their localization in root tissues of field-grown rice plants. | in a previous study by our group, ch4 oxidation and n2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no n input; both processes are likely controlled by a rice gene for microbial symbiosis. the present study examined which microorganisms in rice roots were responsible for ch4 oxidation and n2 fixation under the field conditions. metaproteomic analysis of root-associated bacteria from field-grown rice (oryza sativa nipponbare) revealed that nit ... | 2014 | 24928870 |
| evolution of mitochondria reconstructed from the energy metabolism of living bacteria. | the ancestors of mitochondria, or proto-mitochondria, played a crucial role in the evolution of eukaryotic cells and derived from symbiotic α-proteobacteria which merged with other microorganisms - the basis of the widely accepted endosymbiotic theory. however, the identity and relatives of proto-mitochondria remain elusive. here we show that methylotrophic α-proteobacteria could be the closest living models for mitochondrial ancestors. we reached this conclusion after reconstructing the possibl ... | 2014 | 24804722 |
| biogenic concrete protection driven by the formate oxidation by methylocystis parvus obbp. | the effectiveness of microbiologically induced carbonate precipitation (micp) from the formate oxidation by methylocystis parvus obbp as an alternative process for concrete protection was investigated. micp was induced on autoclaved aerated concrete (aac), the model material, by immersing the material in 10(9) m. parvus cells ml(-1) containing 5 g l(-1) of calcium formate. a 2 days immersion of the material gave the maximum weight increase of the specimens (38 ± 19 mg) and this was likely due to ... | 2015 | 26284061 |
| optimization of methanotrophic growth and production of poly(3-hydroxybutyrate) in a high-throughput microbioreactor system. | production of poly(3-hydroxybutyrate) (p3hb) from methane has economic and environmental advantages over production by agricultural feedstock. identification of high-productivity strains and optimal growth conditions is critical to efficient conversion of methane to polymer. current culture conditions, including serum bottles, shake flasks, and agar plates, are labor-intensive and therefore insufficient for systematic screening and isolation. gas chromatography, the standard method for analysis ... | 2015 | 25956771 |
| first description of sulphur-oxidizing bacterial symbiosis in a cnidarian (medusozoa) living in sulphidic shallow-water environments. | since the discovery of thioautotrophic bacterial symbiosis in the giant tubeworm riftia pachyptila, there has been great impetus to investigate such partnerships in other invertebrates. in this study, we present the occurrence of a sulphur-oxidizing symbiosis in a metazoan belonging to the phylum cnidaria in which this event has never been described previously. | 2015 | 26011278 |
| nitrogenase diversity and activity in the gastrointestinal tract of the wood-eating catfish panaque nigrolineatus. | the amazonian catfish, panaque nigrolineatus, consume large amounts of wood in their diets. the nitrogen-fixing community within the gastrointestinal (gi) tract of these catfish was found to include nifh phylotypes that are closely related to clostridium sp., alpha and gammaproteobacteria, and sequences associated with gi tracts of lower termites. fish fed a diet of sterilized palm wood were found to contain nifh messenger rna within their gi tracts, displaying high sequence similarity to the ni ... | 2015 | 25909976 |
| formations of calcium carbonate minerals by bacteria and its multiple applications. | biomineralization is a naturally occurring process in living organisms. in this review, we discuss microbially induced calcium carbonate precipitation (micp) in detail. in the micp process, urease plays a major role in urea hydrolysis by a wide variety of microorganisms capable of producing high levels of urease. we also elaborate on the different polymorphs and the role of calcium in the formation of calcite crystal structures using various calcium sources. additionally, the environmental facto ... | 2016 | 27026942 |
| the opportunity for high-performance biomaterials from methane. | polyhydroxyalkanoate (pha) biopolymers are widely recognised as outstanding candidates to replace conventional petroleum-derived polymers. their mechanical properties are good and can be tailored through copolymer composition, they are biodegradable, and unlike many alternatives, they do not rely on oil-based feedstocks. further, they are the only commodity polymer that can be synthesised intracellularly, ensuring stereoregularity and high molecular weight. however, despite offering enormous pot ... | 2016 | 27681905 |
| bioenergetic evolution in proteobacteria and mitochondria. | mitochondria are the energy-producing organelles of our cells and derive from bacterial ancestors that became endosymbionts of microorganisms from a different lineage, together with which they formed eukaryotic cells. for a long time it has remained unclear from which bacteria mitochondria actually evolved, even if these organisms in all likelihood originated from the α lineage of proteobacteria. a recent article (degli esposti m, et al. 2014. evolution of mitochondria reconstructed from the ene ... | 2014 | 25432941 |
| methane-oxidizing bacteria in a california upland grassland soil: diversity and response to simulated global change. | we investigated the diversity of methane-oxidizing bacteria (i.e., methanotrophs) in an annual upland grassland in northern california, using comparative sequence analysis of the pmoa gene. in addition to identifying type ii methanotrophs commonly found in soils, we discovered three novel pmoa lineages for which no cultivated members have been previously reported. these novel pmoa clades clustered together either with clone sequences related to "ra 14" or "wb5fh-a," which both represent clusters ... | 2005 | 15870356 |
| genome sequence of the methanotrophic poly-β-hydroxybutyrate producer methylocystis parvus obbp. | methylocystis parvus obbp is an obligate methylotroph considered the type species of the genus methylocystis. two pmocab particulate methane monooxygenase operons and one additional singleton pmoc paralog were identified in the sequence. no evidence of genes encoding soluble methane monooxygenase was found. comparison of m. parvus obbp and methylocystis sp. strain rockwell (atcc 49242) suggests that both species should be taxonomically classified in different genera. | 2012 | 23012286 |
| genome analysis coupled with physiological studies reveals a diverse nitrogen metabolism in methylocystis sp. strain sc2. | methylocystis sp. strain sc2 can adapt to a wide range of methane concentrations. this is due to the presence of two isozymes of particulate methane monooxygenase exhibiting different methane oxidation kinetics. to gain insight into the underlying genetic information, its genome was sequenced and found to comprise a 3.77 mb chromosome and two large plasmids. | 2013 | 24130670 |
| diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoa as molecular marker. | methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. cultivation-dependent and -independent methods have revealed that this functional guild of bacteria comprises a substantial diversity of organisms. in particular the use of cultivation-independent methods targeting a subunit of the particulate methane monooxygenase (pmoa) as functional marker for the detection of aerobic methanotrophs has resulted in thousands of sequences rep ... | 2015 | 26696968 |
| members of the methanotrophic genus methylomarinum inhabit inland mud pots. | proteobacteria capable of converting the greenhouse gas methane to biomass, energy, and carbon dioxide represent a small but important sink in global methane inventories. currently, 23 genera of methane oxidizing (methanotrophic) proteobacteria have been described, although many are represented by only a single validly described species. here we describe a new methanotrophic isolate that shares phenotypic characteristics and phylogenetic relatedness with the marine methanotroph methylomarinum va ... | 2016 | 27478692 |