| cell wall and lipid composition of isosphaera pallida, a budding eubacterium from hot springs. | isosphaera pallida is an unusual gliding, budding eubacterium recently isolated from north american hot springs. electron micrographs of ultrathin sections revealed a cell wall atypical of eubacteria: two electrondense layers separated by an electron-transparent layer, with no evident peptidoglycan layer. growth was not inhibited by penicillin. cell walls were isolated from sheared cells by velocity sedimentation. the rigid-layer fraction, prepared from cell walls by treatment with boiling 10% s ... | 1987 | 3584067 |
| unraveling the extent of diversity within the order planctomycetales. | the phylogenetic positions of 22 isolates that morphologically resemble members of the family planctomycetaceae were determined by sequence analysis of genes coding for 16s rrna. while nine and eight isolates could be assigned to the genera planctomyces and pirellula, respectively, three strains grouped near isosphaera pallida and one strain was closely related to gemmata obscuriglobus. no isolate was found to be related to a previously described species of any of the four genera at the species ... | 1995 | 7793948 |
| phylogenetic diversity, polyamine pattern and dna base composition of members of the order planctomycetales. | the 16s rdna sequences of 20 novel isolates of members of the order planctomycetales were compared to those of the type strains of described planctomycete species and 22 planctomycete isolates for which the 16s rdna sequences had been previously determined. the novel isolates could be assigned to several phylogenetically broad groups, four of which are defined by the genera gemmata, isosphaera, planctomyces and pirellula. to evaluate polyamines as a chemotaxonomic marker within this order, the p ... | 1999 | 10319492 |
| phylogenetic analysis of evolutionary relationships of the planctomycete division of the domain bacteria based on amino acid sequences of elongation factor tu. | sequences from the tuf gene coding for the elongation factor ef-tu were amplified and sequenced from the genomic dna of pirellula marina and isosphaera pallida, two species of bacteria within the order planctomycetales. a near-complete (1140-bp) sequence was obtained from pi. marina and a partial (759-bp) sequence was obtained for i. pallida. alignment of the deduced pi. marina ef-tu amino acid sequence against reference sequences demonstrated the presence of a unique 11-amino acid sequence moti ... | 2001 | 11443344 |
| cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. | the organisation of cells of the planctomycete species pirellula marina, isosphaera pallida, gemmata obscuriglobus, planctomyces maris and "candidatus brocadia anammoxidans" was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of gemmata obscuriglobus and pirellula marina, computer-aided 3-d reconstructions from serial sections of cryosubstituted cells. all planctomycete cells display a peripheral ribosome-free re ... | 2001 | 11491082 |
| isolation of gemmata-like and isosphaera-like planctomycete bacteria from soil and freshwater. | new cultured strains of the planctomycete division (order planctomycetales) of the domain bacteria related to species in the genera gemmata and isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. phylogenetic analysis of the 16s rrna gene from eight representative isolates showed that all the isolates were members of the planctomycete division. six isolates clustered with gemmata obscuriglobus and related strains, while two isolates clustered with isosphaera pal ... | 2002 | 11772655 |
| molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant. | we examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. three 16s rrna gene libraries were constructed by using a 16s rrna-targeted universal reverse primer and a forward pcr primer specific for planctomyces: phylogenetic analysis of 234 16s rrna gene sequences defined 110 operational taxonomic units. the majority of these sequences clustered with the four known genera, pirellula (32%), plan ... | 2003 | 14660385 |
| [high abundance of planctomycetes in anoxic layers of a sphagnum peat bog]. | the depth distribution of planctomycete abundance has been examined in six different sites of the sphagnum peat bog in bakchar, tomsk oblast, russia. in situ hybridization of peat with the fluorescently labeled oligonucleotide probes pla46 and pla886, reported to be group-specific for representatives of the phylum planctomycetes, revealed two distinct population maxima of these bacteria in all of the profiles examined. the first population maximum was detected in the uppermost, oxic layer of the ... | 2006 | 17205808 |
| complete genome sequence of isosphaera pallida type strain (is1b). | isosphaera pallida (ex woronichin 1927) giovannoni et al. 1995 is the type species of the genus isosphaera. the species is of interest because it was the first heterotrophic bacterium known to be phototactic, and it occupies an isolated phylogenetic position within the planctomycetaceae. here we describe the features of this organism, together with the complete genome sequence and annotation. this is the first complete genome sequence of a member of the genus isosphaera and the third of a member ... | 2011 | 21475588 |
| aquisphaera giovannonii gen. nov., sp. nov. a novel planctomycete isolated from a freshwater aquarium. | as part of a study of the diversity of planctomycetes we isolated two strains designated ojf2(t) and ojf8, from the sediments of a freshwater aquarium. the organisms were chemoheterotrophic, spherical-shaped and pink-pigmented, had a optimum growth temperature of about 30-35 °c and an optimum ph for growth of around 7.5-8.5. the fatty acids are primarily composed of c(18:1) ω9c and c(16:0). the new organisms assimilate several sugars, and organic acids. the 16s rdna sequence analysis confirmed t ... | 2011 | 21239565 |
| singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, isosphaera-like planctomycete from acidic northern wetlands. | four novel strains of budding bacteria, designated mob10t, po2, mpl1015 and bg32, were isolated from acidic wetlands of northern russia. cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. the isolates were moderately acidophilic, mesophilic organisms capable of growth between ph 4.2 and 7.5 (optimum growth at ph 5.0-6.2) and at temperatures between 4 and 33 degrees c (optimum growt ... | 2008 | 18450711 |
| comparative analysis of ribonuclease p rna of the planctomycetes. | the planctomycetes, order planctomycetales, are a distinct phylum of domain bacteria. genes encoding the rna portion of ribonuclease p (rnase p) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. rnpb gene sequences encoding rnase p rna were generated by a conserved primer pcr strategy for planctomyces brasiliensis, planctomyces limnophilus, pirellula marina, pirellula staleyi strain atcc 35122, isosphaera pallida, one other isosphaera strai ... | 2004 | 15280311 |
| phylogeny of the filamentous bacterium 'nostocoida limicola' iii from activated sludge. | five strains of the filamentous bacterium 'nostocoida limicola' iii were successfully isolated into pure culture from samples of activated sludge biomass from five plants in australia. 16s rrna gene sequence analyses showed that all isolates were members of the planctomycetales, most closely related to isosphaera pallida, but they differed phenotypically from this species in that they did not glide and were not thermotolerant. the ultrastructure of these 'n. limicola' iii isolates was also consi ... | 2001 | 11211260 |
| response of soybean rhizosphere communities to human hygiene water addition as determined by community level physiological profiling (clpp) and terminal restriction fragment length polymorphism (trflp) analysis. | in this report, we describe an experiment conducted at kennedy space center in the biomass production chamber (bpc) using soybean plants for purification and processing of human hygiene water. specifically, we tested whether it was possible to detect changes in the root-associated bacterial assemblage of the plants and ultimately to identify the specific microorganism(s) which differed when plants were exposed to hygiene water and other hydroponic media. plants were grown in hydroponics media co ... | 2000 | 10689173 |
| a unique type of eubacterial 5s rrna in members of the order planctomycetales. | analysis of the 5s ribosomal rna from members of the eubacterial order planctomycetales, i.e., planctomyces, pirella, gemmata, and isosphaera, reveals several unexpected features. firstly, the primary structures are significantly shorter than those of the majority of eubacteria and vary in length between 109 and 111 nucleotides. secondly, the lack of an insertion at position 66 is a feature not encountered before in prokaryotic 5s rrnas. thirdly, as compared to the proposed eubacterial "minimal" ... | 1988 | 3137349 |