| molecular and ultrastructural confirmation of classification of atcc 35122 as a strain of pirellula staleyi. | a freshwater isolate from campus lake, baton rouge, la, usa, strain atcc 35122 (= icpb 4362 = schmidt clpm white = tekniepe bt2 white), which had been proposed as a putative reference strain for 'planctomyces staleyi' (later reclassified as pirellula staleyi), has been re-examined to establish its relationship to the type strain of pirellula staleyi, atcc 27377t. 165 rrna sequencing confirms its very close relationship to atcc 27377t and its membership of the order planctomycetales. ultrastructu ... | 2002 | 12361271 |
| molecular characterization of bacterial diversity from british columbia forest soils subjected to disturbance. | bacteria from forest soils were characterized by dna sequence analysis of cloned 16s rrna gene fragments (16s clones). surface organic matter and mineral soil samples from a british columbia ministry of forests long-term soil productivity (ltsp) installation were collected during winter and summer from two disturbance treatments: whole-tree harvesting with no soil compaction (plot n) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot s). phylo ... | 2002 | 12224564 |
| long-term succession of structure and diversity of a biofilm formed in a model drinking water distribution system. | in this study, we examined the long-term development of the overall structural morphology and community composition of a biofilm formed in a model drinking water distribution system with biofilms from 1 day to 3 years old. visualization and subsequent quantification showed how the biofilm developed from an initial attachment of single cells through the formation of independent microcolonies reaching 30 micro m in thickness to a final looser structure with an average thickness of 14.1 micro m and ... | 2003 | 14602654 |
| 16s-23s rdna intergenic spacer and 23s rdna of anaerobic ammonium-oxidizing bacteria: implications for phylogeny and in situ detection. | recently, anaerobic ammonium-oxidizing bacteria (aaob) were identified by comparative 16s rdna sequence analysis as a novel, deep-branching lineage within the planctomycetales. this lineage consists currently of only two, not yet culturable bacteria which have been provisionally described as candidatus 'brocadia anammoxidans' and candidatus 'kuenenia stuttgartiensis'. in this study, a large fragment of the rdna operon, including the 16s rdna, the intergenic spacer region (isr) and approximately ... | 2001 | 11553235 |
| a phylogenetic definition of the major eubacterial taxa. | through oligonucleotide signature analysis of 16s ribosomal rnas, it is possible to define ten major groups of eubacteria. these are: (1) the gram positive bacteria, (2) the purple photosynthetic bacteria and their relatives, (3) the spirochetes and their relatives, (4) the sulfur-dependent eubacteria and their relatives, (5) the bacteroides, flavobacteria and cytophagas and their relatives, (6) the cyanobacteria, (7) the green sulfur bacteria, (8) the green non-sulfur bacteria and their relat ... | 1985 | 11542017 |
| cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. | the organisation of cells of the planctomycete species pirellula marina, isosphaera pallida, gemmata obscuriglobus, planctomyces maris and "candidatus brocadia anammoxidans" was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of gemmata obscuriglobus and pirellula marina, computer-aided 3-d reconstructions from serial sections of cryosubstituted cells. all planctomycete cells display a peripheral ribosome-free re ... | 2001 | 11491082 |
| diversity of free-living prokaryotes from a deep-sea site at the antarctic polar front. | to contribute to the understanding of deep-sea planktonic communities, we explored the prokaryotic diversity of a 3000 m deep site at the antarctic polar front using molecular methods. bacterial 16s rdna-amplified sequences corresponded to the as yet uncultivated groups sar11, within the alpha-proteobacteria, and sar324, within the delta-proteobacteria, as well as to the gamma-proteobacteria, cytophagales, planctomyces, gram-positives, and the group of environmental sequences sar406. among them, ... | 2001 | 11451524 |
| molecular analysis of microbial communities in mobile deltaic muds of southeastern papua new guinea. | a culture-independent examination of microbial diversity in mobile deltaic sediments from the gulf of papua, papua new guinea, was conducted by sequence analysis of 16s rdna clone library. universal small subunit primers were used to amplify dna extracted from the sediment. of 91 clones randomly selected from the library, 33 contained unique non-chimeric sequences. analysis of these unique sequences showed that the majority of them belonged to bacteria (94.5%), with proteobacteria being the domi ... | 2000 | 10967214 |
| a search for beta-lactamase in chlamydiae, mycoplasmas, planctomycetes, and cyanelles: bacteria and bacterial descendants at different phylogenetic positions and stages of cell wall development. | bacteria from different phylogenetic positions such as chlamydiae, mycoplasmas, planctomycetes and also endosymbiotic murein-containing cyanelles were investigated for the production of beta-lactamases. no beta-lactamase activity was found in bacteria lacking murein such as chlamydia pneumoniae, mycoplasma pneumoniae, pirellula marina and planctomyces maris. in the murein-containing cyanelles of cyanophora paradoxa no beta-lactamase activity could be detected. | 2000 | 10830893 |
| phylogenetic diversity, polyamine pattern and dna base composition of members of the order planctomycetales. | the 16s rdna sequences of 20 novel isolates of members of the order planctomycetales were compared to those of the type strains of described planctomycete species and 22 planctomycete isolates for which the 16s rdna sequences had been previously determined. the novel isolates could be assigned to several phylogenetically broad groups, four of which are defined by the genera gemmata, isosphaera, planctomyces and pirellula. to evaluate polyamines as a chemotaxonomic marker within this order, the p ... | 1999 | 10319492 |
| cloning and characterization of planctomyces limnophilus rpon: complementation of a salmonella typhimurium rpon mutant strain. | the rpon gene, which encodes the alternative sigma factor sigma 54, was cloned from the budding, peptidoglycan-less bacterium planctomyces limnophilus. p. limnophilus rpon complemented the ntr- phenotype of a salmonella typhimurium rpon mutant strain. the p. limnophilus rpon gene encoded a predicted polypeptide that was 495 residues in length and shared a significant homology with other members of the sigma 54 family. the protein sequence displayed all of the characteristic motifs found in membe ... | 1998 | 9852960 |
| screening of a fosmid library of marine environmental genomic dna fragments reveals four clones related to members of the order planctomycetales. | a fosmid library with inserts containing approximately 40 kb of marine bacterial dna (j. l. stein, t. l. marsh, k. y. wu, h. shizuya, and e. f. delong, j. bacteriol. 178:591-599, 1996) yielded four clones with 16s rrna genes from the order planctomycetales. three of the clones belong to the pirellula group and one clone belongs to the planctomyces group, based on phylogenetic and signature nucleotide analyses of full-length 16s rrna genes. sequence analysis of the ends of the genes revealed a co ... | 1998 | 9687477 |
| the presence of a dnak (hsp70) multigene family in members of the orders planctomycetales and verrucomicrobiales. | sequences of the dnak gene, coding for the 70-kda heat shock protein (hsp70), were determined for six members of the order planctomycetales, including representatives of three genera, and for the only cultivated member of the order verrucomicrobiales, verrucomicrobium spinosum. a fragment of the dnak gene was amplified from these strains by pcr with oligonucleotide primers targeting regions of the dnak gene that are conserved at the amino acid level, and the resulting pcr products were cloned in ... | 1997 | 9335284 |
| phylogenetic analysis and in situ identification of bacteria in activated sludge. | the bacterial community structure of activated sludge of a large municipal wastewater treatment plant was investigated by use of the rrna approach. almost-full-length genes coding for the small-subunit rrna (rdna) were amplified by pcr and subsequently cloned into the pgem-t vector. clones were screened by dot blot hybridization with group-specific oligonucleotide probes. the phylogenetic affiliations of clones were compared with the results obtained with the original sample by in situ hybridiza ... | 1997 | 9212435 |
| molecular microbial diversity of an anaerobic digestor as determined by small-subunit rdna sequence analysis. | the bacterial community structure of a fluidized-bed reactor fed by vinasses (wine distillation waste) was analyzed. after pcr amplification, four small-subunit (ssu) rdna clone libraries of bacteria, archaea, procarya, and eucarya populations were established. the community structure was determined by operational taxonomic unit (otu) phylogenetic analyses of 579 partial rdna sequences (about 500 bp long). a total of 146 otus were found, comprising 133, 6, and 7 from the bacteria, archaea, and e ... | 1997 | 9212428 |
| bacterial diversity of a carolina bay as determined by 16s rrna gene analysis: confirmation of novel taxa. | carolina bays are naturally occurring shallow elliptical depressions largely fed by rain and shallow ground water. to identify members of the domain bacteria which inhibit such an environment, we used pcr to construct a library of 16s rrna genes (16s rdnas) cloned from dna extracted from the sediments of rainbow bay, located on the savannah river site, near aiken, s.c. oligonucleotides complementary to conserved regions of 16s rdna were used as primers for pcr, and gel-purified pcr products were ... | 1997 | 9097448 |
| isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, penaeus monodon. | bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain bacteria were isolated from postlarvae of the giant tiger prawn, penaeus monodon. a selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. planctomycetes were isolated from both healthy and monodon baculovirus-infected prawn postlarvae. the predominant colony type recovered from postlarvae regardless of viral infection s ... | 1997 | 8979353 |
| phylogenetic analysis of the bacterial communities in marine sediments. | for the phylogenetic analysis of microbial communities present in environmental samples microbial dna can be extracted from the sample, 16s rdna can be amplified with suitable primers and the pcr, and clonal libraries can be constructed. we report a protocol that can be used for efficient cell lysis and recovery of dna from marine sediments. key steps in this procedure include the use of a bead mill homogenizer for matrix disruption and uniform cell lysis and then purification of the released dn ... | 1996 | 8899989 |
| phylogeny of prosthecobacter, the fusiform caulobacters: members of a recently discovered division of the bacteria. | prosthecobacter fusiformis is morphologically similar to caulobacters; however, it lacks a dimorphic life cycle. to determine the relatedness of the genus prosthecobacter to dimorphic caulobacters and other prosthecate members of the alpha subgroup of the proteobacteria (alpha-proteobacteria), we isolated and sequenced 16s rrna genes from four prosthecobacter strains. surprisingly, the results of phylogenetic analyses placed the fusiform caulobacters in a deeply rooted division of the bacteria t ... | 1996 | 8863424 |
| estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative pcr method. | strain ea25 was identified in a clone library of bacterial 16s rrna gene sequences that had been amplified from dna extracted from soil collected in eastern washington state. ea25 was subsequently shown to be related to members of the genera planctomyces and chlamydia and most closely related (93% similarity) to strain mc18, a strain identified in an australian soil sample (w. liesack and e. stackebrandt, j. bacteriol. 174:5072-5078, 1992). a competitive quantitative pcr method developed by zach ... | 1996 | 8837435 |
| physical map of the genome of planctomyces limnophilus, a representative of the phylogenetically distinct planctomycete lineage. | a physical map of the chromosome of planctomyces limnophilus dsm 3776t was constructed by pulsed-field gel electrophoresis techniques. a total of 32 cleavage sites for the rare-cutting restriction endonucleases paci, pmei, and swai were located on the chromosome, which was shown to be circular and approximately 5.2 mbp in size. an extrachromosomal element was detected but was found not to be cleaved by any of the enzymes used in the analysis of the chromosome. the order of the fragments on the c ... | 1996 | 8606164 |
| unraveling the extent of diversity within the order planctomycetales. | the phylogenetic positions of 22 isolates that morphologically resemble members of the family planctomycetaceae were determined by sequence analysis of genes coding for 16s rrna. while nine and eight isolates could be assigned to the genera planctomyces and pirellula, respectively, three strains grouped near isosphaera pallida and one strain was closely related to gemmata obscuriglobus. no isolate was found to be related to a previously described species of any of the four genera at the species ... | 1995 | 7793948 |
| phylogenetic position of the menaquinone-containing acidophilic chemo-organotroph acidobacterium capsulatum. | the phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, acidobacterium capsulatum, was studied on the basis of 16s rrna gene sequence information. a. capsulatum showed the highest level of sequence similarity to heliobacterium chlorum, a member of the gram-positive group, yet this level was only 81%. distance matrix tree analysis suggested that a. capsulatum belongs to a unique lineage deeply branching from the chlamydia-planctomyces group or from the gr ... | 1995 | 7590170 |
| molecular genetic evidence for early evolutionary origin of budding peptidoglycan-less eubacteria. | recent studies on the cell wall composition of budding, non-prosthecate bacteria have shown that representatives of the genera planctomyces and pasteuria (sensu staley, 1973) lack the peptidoglycan moiety, which is the characteristic feature of eubacterial cell walls, but possess an as yet unidentified protein sheet instead; in this respect planctomyces and pasteuria strains resemble certain archaebacteria more than eubacteria. to determine their phylogenetic positions, the 16s ribosomal rnas of ... | 1984 | 6700702 |
| isolation of a bacterium resembling pirellula species from primary tissue culture of the giant tiger prawn (penaeus monodon). | during attempts to establish tissue cultures from hepatopancreas, heart, and hemolymph of the giant tiger prawn (penaeus monodon), using a medium including penicillin, streptomycin, and amphotericin b, bacterial contamination in the form of a sheet of growth attached to the tissue culture vessel was a persistent problem. contaminant bacteria were teardrop-shaped cells arranged in rosettes, and electron microscopy revealed buds, crateriform structures, and the absence of a peptidoglycan layer in ... | 1991 | 1781677 |
| soil microbial community structure across a thermal gradient following a geothermal heating event. | in this study microbial species diversity was assessed across a landscape in yellowstone national park, where an abrupt increase in soil temperature had occurred due to recent geothermal activity. soil temperatures were measured, and samples were taken across a temperature gradient (35 to 65 degrees c at a 15-cm depth) that spanned geothermally disturbed and unimpacted soils; thermally perturbed soils were visually apparent by the occurrence of dead or dying lodgepole pine trees. changes in soil ... | 2002 | 12450855 |
| molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant. | we examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. three 16s rrna gene libraries were constructed by using a 16s rrna-targeted universal reverse primer and a forward pcr primer specific for planctomyces: phylogenetic analysis of 234 16s rrna gene sequences defined 110 operational taxonomic units. the majority of these sequences clustered with the four known genera, pirellula (32%), plan ... | 2003 | 14660385 |
| comparative analysis of ribonuclease p rna of the planctomycetes. | the planctomycetes, order planctomycetales, are a distinct phylum of domain bacteria. genes encoding the rna portion of ribonuclease p (rnase p) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. rnpb gene sequences encoding rnase p rna were generated by a conserved primer pcr strategy for planctomyces brasiliensis, planctomyces limnophilus, pirellula marina, pirellula staleyi strain atcc 35122, isosphaera pallida, one other isosphaera strai ... | 2004 | 15280311 |
| diversity and seasonal changes of uncultured planctomycetales in river biofilms. | cell counts of planctomycetes showed that there were high levels of these organisms in the summer and low levels in the winter in biofilms grown in situ in two polluted rivers, the elbe river and the spittelwasser river. in this study 16s rrna-based methods were used to investigate if these changes were correlated with changes in the species composition. planctomycete-specific clone libraries of the 16s rrna genes found in both rivers showed that there were seven clusters, which were distantly r ... | 2004 | 15345387 |
| influence of an oyster reef on development of the microbial heterotrophic community of an estuarine biofilm. | we characterized microbial biofilm communities developed over two very closely located but distinct benthic habitats in the pensacola bay estuary using two complementary cultivation-independent molecular techniques. biofilms were grown for 7 days on glass slides held in racks 10 to 15 cm over an oyster reef and an adjacent muddy sand bottom. total biomass and optical densities of dried biofilms showed dramatic differences for oyster reef versus non-oyster reef biofilms. this study assessed wheth ... | 2004 | 15528551 |
| the nomenclatural types of the orders acholeplasmatales, halanaerobiales, halobacteriales, methanobacteriales, methanococcales, methanomicrobiales, planctomycetales, prochlorales, sulfolobales, thermococcales, thermoproteales and verrucomicrobiales are the genera acholeplasma, halanaerobium, halobacterium, methanobacterium, methanococcus, methanomicrobium, planctomyces, prochloron, sulfolobus, thermococcus, thermoproteus and verrucomicrobium, respectively. opinion 79. | the judicial commission of the international committee on systematics of prokaryotes has corrected the nomenclatural types of 12 orders: acholeplasmatales, halanaerobiales, halobacteriales, methanobacteriales, methanococcales, methanomicrobiales, planctomycetales, prochlorales, sulfolobales, thermococcales, thermoproteales and verrucomicrobiales. | 2005 | 15653928 |
| "who has first observed planctomyces" (or data to the history of planctomyces bekefii). | the first description of planctomyces bekefii was published in eighty years ago by n. gimesi in hungary. the history and importance of this species is revealed in this article. | 2005 | 15957235 |
| vertical distribution and diversity of bacteria and archaea in sulfide and methane-rich cold seep sediments located at the base of the florida escarpment. | the bacterial and archaeal communities of the sediments at the base of the florida escarpment (gulf of mexico, usa) were investigated using molecular phylogenetic analysis. the total microbial community dna of each of three vertical zones (top, middle and bottom) of a sediment core was extracted and the 16s rrna genes were amplified by pcr, cloned and sequenced. shannon-weaver diversity measures of bacteria were high in all three zones. for the archaea, diversity was generally low, but increased ... | 2006 | 16465452 |
| [detection of representatives of the planctomycetes in sphagnum peat bogs by molecular and cultivation methods]. | by means of fluorescence in situ hybridization with 16s rrna-targeted oligonucleotide probes (fish), it has been shown that members of the phylum planctomycetes represent a numerically significant bacterial group in boreal sphagnum peat bogs. the population size of planctomycetes in oxic layers of the peat bog profile was in the range of 0.4-2.0 x 10(7) cells per g of wet peat, comprising 4 to 13% of the total bacterial cell number. a novel effective approach that combined a traditional cultivat ... | 2006 | 16871807 |
| [high abundance of planctomycetes in anoxic layers of a sphagnum peat bog]. | the depth distribution of planctomycete abundance has been examined in six different sites of the sphagnum peat bog in bakchar, tomsk oblast, russia. in situ hybridization of peat with the fluorescently labeled oligonucleotide probes pla46 and pla886, reported to be group-specific for representatives of the phylum planctomycetes, revealed two distinct population maxima of these bacteria in all of the profiles examined. the first population maximum was detected in the uppermost, oxic layer of the ... | 2006 | 17205808 |
| characterization of the microbial diversity in a permafrost sample from the canadian high arctic using culture-dependent and culture-independent methods. | a combination of culture-dependent and culture-independent methodologies (bacteria and archaea 16s rrna gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high arctic permafrost sample. culturable bacteria isolates, identified by 16s rrna gene sequencing, belonged to the phyla firmicutes, actinobacteria and proteobacteria with spore-forming firmicutes being the most abundant; the majority of the isolates (19/23) were psychrotoleran ... | 2007 | 17313587 |
| schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order planctomycetales, from sphagnum-dominated boreal wetlands. | three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic sphagnum-dominated boreal wetlands of northern russia and were designated strains mpl7t, mob77 and sb2. the presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. these isolates are moderately acidophilic, mesophilic organisms capable of growth at ph values between 4.2 and 7. ... | 2007 | 17978240 |
| fosmids of novel marine planctomycetes from the namibian and oregon coast upwelling systems and their cross-comparison with planctomycete genomes. | planctomycetes are widely distributed in marine environments, where they supposedly play a role in carbon recycling. to deepen our understanding about the ecology of this sparsely studied phylum six planctomycete fosmids from two marine upwelling systems were investigated and compared with all available planctomycete genomic sequences including the as yet unpublished near-complete genomes of blastopirellula marina dsm 3645(t) and planctomyces maris dsm 8797(t). high numbers of sulfatase genes (4 ... | 2007 | 18043661 |
| application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal. | the simultaneous ammonium and sulfate removal was detected in an anammox reactor, consisted of ammonium oxidization with sulfate deoxidization, and subsequently traditional anammox process, in via of middle medium nitrite with solid sulfur and n2 as the terminal products. the pure anammox bacteria offered a great biotechnological potential for the completely autotrophic reaction indicated by batch tests. denaturing gradient gel electrophoresis (dgge) analysis further revealed that a new organism ... | 2008 | 18343660 |
| phylogenetic diversity of bacteria associated with the mucus of red sea corals. | coral reefs are the most biodiverse and biologically productive of all marine ecosystems. corals harbor diverse and abundant prokaryotic communities. however, little is known about the diversity of coral-associated bacterial communities. mucus is a characteristic product of all corals, forming a coating over their polyps. the coral mucus is a rich substrate for microorganisms. mucus was collected with a procedure using sterile cotton swabs that minimized contamination of the coral mucus by surro ... | 2008 | 18355296 |
| different planctomycetes diversity patterns in latitudinal surface seawater of the open sea and in sediment. | the 16s rrna gene approach was applied to investigate the diversity of planctomycetes in latitudinal surface seawater of the western pacific ocean. the results revealed that the pirellula-rhodopirellula-blastopirellula clade dominated the planctomycetes community at all surface seawater sites while the minority genera gemmata and planctomyces were only found at sites h5 and h2 respectively. although the clone frequency of the prb clade seemed stable (between 83.3% and 94.1%) for all surface seaw ... | 2008 | 18545964 |
| diversity of aerobic and anaerobic ammonia-oxidizing bacteria in marine sponges. | aerobic ammonia-oxidizing bacteria (aaob) are known to have an important function in the marine nitrogen cycle. anaerobic ammonium oxidation (anammox) carried out by some members of planctomycetales is also an important process in marine ecosystems. ammonia-monooxygenase gene (amoa) fragments were amplified to investigate the potential for nitrification and the diversity of the aaob in two marine sponges ircinia strobilina and mycale laxissima. all of the amoa sequences obtained from the two spo ... | 2010 | 19617876 |
| phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of phycisphaeraceae fam. nov., phycisphaerales ord. nov. and phycisphaerae classis nov. in the phylum planctomycetes. | three strains, fyk2301m01(t), fyk2301m18 and fyk2301m52, all being gram-negative, spherical, motile and facultatively anaerobic, were isolated from a marine alga (porphyra sp.) collected on mikura island, japan. colonies of the strains were circular and pink-pigmented on marine agar 2216 (difco) at 25 degrees c. cells of the strains reproduced by binary fission. the g+c content of the dna was 73 mol%. the major isoprenoid quinone was mk-6. phylogenetic analysis based on 16s rrna gene sequences i ... | 2009 | 19700920 |
| aerobic and anaerobic ammonia oxidizing bacteria--competitors or natural partners? | the biological nitrogen cycle is a complex interplay between many microorganisms catalyzing different reactions. for a long time, ammonia and nitrite oxidation by chemolithoautotrophic nitrifiers were thought to be restricted to oxic environments and the metabolic flexibility of these organisms seemed to be limited. the discovery of a novel pathway for anaerobic ammonia oxidation by planctomyces (anammox) and the finding of an anoxic metabolism by 'classical'nitrosomonas-like organisms showed th ... | 2002 | 19709197 |
| bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (zea mays) and comparison to its non-transgenic cultivar bosphore. | bacterial communities in rhizospheres of transgenic maize (zea mays, with the pat-gene conferring resistance to the herbicide glufosinate; syn. l-phosphinothricin) were compared to its isogenic, non-transgenic cultivar. total dna was extracted from bacterial cell consortia collected from rhizospheres of plants grown in an agricultural field. with the use of three different primer pairs binding to evolutionarily conserved regions of the bacterial 16s rrna gene, partial sequences were amplified by ... | 2002 | 19709208 |
| widespread head-to-head hydrocarbon biosynthesis in bacteria and role of olea. | previous studies identified the oleabcd genes involved in head-to-head olefinic hydrocarbon biosynthesis. the present study more fully defined the oleabcd protein families within the thiolase, alpha/beta-hydrolase, amp-dependent ligase/synthase, and short-chain dehydrogenase superfamilies, respectively. only 0.1 to 1% of each superfamily represents likely ole proteins. sequence analysis based on structural alignments and gene context was used to identify highly likely ole genes. selected microor ... | 2010 | 20418421 |
| characterization of planctomyces limnophilus and development of genetic tools for its manipulation establish it as a model species for the phylum planctomycetes. | planctomycetes represent a remarkable clade in the domain bacteria because they play crucial roles in global carbon and nitrogen cycles and display cellular structures that closely parallel those of eukaryotic cells. studies on planctomycetes have been hampered by the lack of genetic tools, which we developed for planctomyces limnophilus. | 2011 | 21724885 |
| Planctomycetes diversity associated with macroalgae. | Planctomycetes associated with 12 macroalgae from the north coast of Portugal were isolated, using an improved method. A total of 138 isolates were found to comprise 10 operational taxonomic units (OTUs), with 65% of the strains being closely related to the species Rhodopirellula baltica. The other strains are probably new species or genera related to Rhodopirellula, Blastopirellula and Planctomyces. Some of the OTUs isolated are unique and have never been found before in previous studies. Catal ... | 2011 | 21726244 |
| broad-spectrum antibiotic resistance of planctomycetes organisms determined by etest. | the in vitro susceptibility of planctomycetes organisms to antibiotics has seldom been studied and when it has, a variety of methods have been used. the objective of the study was to expand the knowledge of planctomycetes antibiotic susceptibility patterns. | 2010 | 20699245 |
| planctomycetes dominate biofilms on surfaces of the kelp laminaria hyperborea. | bacteria belonging to planctomycetes display several unique morphological and genetic features and are found in a wide variety of habitats on earth. their ecological roles in these habitats are still poorly understood. planctomycetes have previously been detected throughout the year on surfaces of the kelp laminaria hyperborea from southwestern norway. we aimed to make a detailed investigation of the abundance and phylogenetic diversity of planctomycetes inhabiting these kelp surfaces. | 2010 | 20950420 |
| complete genome sequence of pirellula staleyi type strain (atcc 27377). | pirellula staleyi schlesner and hirsch 1987 is the type species of the genus pirellula of the family planctomycetaceae. members of this pear- or teardrop-shaped bacterium show a clearly visible pointed attachment pole and can be distinguished from other planctomycetes by a lack of true stalks. strains closely related to the species have been isolated from fresh and brackish water, as well as from hypersaline lakes. here we describe the features of this organism, together with the complete genome ... | 2009 | 21304671 |
| complete genome sequence of planctomyces limnophilus type strain (mü 290). | planctomyces limnophilus hirsch and müller 1986 belongs to the order planctomycetales, which differs from other bacterial taxa by several distinctive features such as internal cell compartmentalization, multiplication by forming buds directly from the spherical, ovoid or pear-shaped mother cell and a cell wall which is stabilized by a proteinaceous layer rather than a peptidoglycan layer. besides pirellula staleyi, this is the second completed genome sequence of the family planctomycetaceae. p. ... | 2010 | 21304691 |
| temporal variability of coastal planctomycetes clades at station kabeltonne, north sea. | members of the bacterial phylum planctomycetes are reported in marine water samples worldwide but quantitative information is scarce. here we investigated the phylogenetic diversity, abundance and distribution of planctomycetes in surface waters off the german north sea island helgoland during different seasons by 16s ribosomal rna (rrna) gene analysis and catalyzed reporter deposition fluorescence in situ hybridization (card-fish). generally planctomycetes are more abundant in summer and autumn ... | 2011 | 21642408 |
| a unique type of eubacterial 5s rrna in members of the order planctomycetales. | analysis of the 5s ribosomal rna from members of the eubacterial order planctomycetales, i.e., planctomyces, pirella, gemmata, and isosphaera, reveals several unexpected features. firstly, the primary structures are significantly shorter than those of the majority of eubacteria and vary in length between 109 and 111 nucleotides. secondly, the lack of an insertion at position 66 is a feature not encountered before in prokaryotic 5s rrnas. thirdly, as compared to the proposed eubacterial "minimal" ... | 1988 | 3137349 |
| eubacterial origin of chlamydiae. | the sequence of the 16s rrna gene from chlamydia psittaci was determined. comparison of this sequence with other 16s rrna sequences showed the organism to be eubacterial. the organism represents a hitherto unrecognized major eubacterial group. however, this group may be peripherally related to the planctomyces and relatives. although these two groups seem to have very little in common phenotypically (they have been studied in very different ways), cell walls in both cases contain no peptidoglyca ... | 1986 | 3525517 |