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oxygen and carbon monoxide equilibria and the kinetics of oxygen binding by the cooperative dimeric hemoglobin of thyonella gemmata. 1979497157
kinetics of carbon monoxide binding to the cooperative dimeric hemoglobin from thyonella gemmata. analysis of carbon monoxide equilibrium results. 1979497158
toxic metabolites of amanita pantherina, a. cothurnata, a. muscaria and other amanita species.forty-seven specimens representing 35 species or varieties of amanita were examined for the presence of ibotenic acid, muscimol, stizolobic acid, stizolobinic acid, aminohexadienoic acid and chlorocrotylglycine. in addition, crude extracts of a. muscaria were examined chromatographically for the presence of methyltetrahydrocarboline carboxylic acid (mtc). ibotenic acid and muscimol were found in clearly detectable concentrations in extracts of a. cothurnata, all specimens of a. muscaria, all spe ...1976985999
accumulation of vanadium during embryogenesis in the vanadium-rich ascidian, ascidia gemmata.it is a remarkable and previously unrecognized fact that ascidians, which are known to contain high levels of vanadium in their blood cells, begin to accumulate vanadium during embryogenesis. this study revealed that the accumulation starts quite dramatically 2 wk after fertilization, and 2 mo later, the amount of vanadium in larvae is 600,000 times higher than that in the unfertilized egg. these results were obtained by neutron activation analysis, a highly sensitive method for determining leve ...19921384612
a unique type of eubacterial 5s rrna in members of the order planctomycetales.analysis of the 5s ribosomal rna from members of the eubacterial order planctomycetales, i.e., planctomyces, pirella, gemmata, and isosphaera, reveals several unexpected features. firstly, the primary structures are significantly shorter than those of the majority of eubacteria and vary in length between 109 and 111 nucleotides. secondly, the lack of an insertion at position 66 is a feature not encountered before in prokaryotic 5s rrnas. thirdly, as compared to the proposed eubacterial "minimal" ...19883137349
isolation and identification of flavonoids from leaves of celastrus scandens l., c. rugosa rehd. et wills., c. gemmata loes. and c. loeseneri rehd. et wills. 19734774251
sea cucumber sibling species: polypeptide chain types and oxygen equilibrium of hemoglobin.the hemoglobin of the "thin" sibling species of thyonella gemmata (phylum: echinodermata; class: holothuria) has three electrophoretically distinct polypeptide chains. in "stout" sibling species of t. gemmata there are only two chain types. these results account for the greater number of multiple hemoglobins in "thins" than in "stouts," as well as for differences in the amounts of some of the multiple hemoglobins when comparisons are mnade of hemolyzates of erythrocytes from the water vascular s ...19665937135
gemmata obscuriglobus, a new genus and species of the budding bacteria.a single strain of a budding bacterium was isolated from freshwater. the strain had a life-cycle, with a multitrichous swarmer stage, and produced a phase-dark inclusion of packed ribosomes and nuclear material. the mol % g + c of the dna was 64.4 +/- 1.0. a new genus, gemmata with the type species gemmata obscuriglobus is proposed. the type strain is uqm 2246.19846486770
[mushroom poisoning in the ix region. role of amanita gemmata].we report four episodes of mushroom poisoning that occurred between 1986 and 1990 in the province of malleco. twenty five of 36 individuals who ingested the mushroom became ill; they had an acute gastroenteritis that was followed in 7 by an acute hepatitis and in one by a massive upper gastrointestinal bleeding. three subjects with fulminant hepatic failure and the subject with the massive bleeding died. amanita gemmate (strain described as toxic in chile since 1967) was found in two episodes an ...19947732230
unraveling the extent of diversity within the order planctomycetales.the phylogenetic positions of 22 isolates that morphologically resemble members of the family planctomycetaceae were determined by sequence analysis of genes coding for 16s rrna. while nine and eight isolates could be assigned to the genera planctomyces and pirellula, respectively, three strains grouped near isosphaera pallida and one strain was closely related to gemmata obscuriglobus. no isolate was found to be related to a previously described species of any of the four genera at the species ...19957793948
isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, penaeus monodon.bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain bacteria were isolated from postlarvae of the giant tiger prawn, penaeus monodon. a selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. planctomycetes were isolated from both healthy and monodon baculovirus-infected prawn postlarvae. the predominant colony type recovered from postlarvae regardless of viral infection s ...19978979353
phylogenetic diversity, polyamine pattern and dna base composition of members of the order planctomycetales.the 16s rdna sequences of 20 novel isolates of members of the order planctomycetales were compared to those of the type strains of described planctomycete species and 22 planctomycete isolates for which the 16s rdna sequences had been previously determined. the novel isolates could be assigned to several phylogenetically broad groups, four of which are defined by the genera gemmata, isosphaera, planctomyces and pirellula. to evaluate polyamines as a chemotaxonomic marker within this order, the p ...199910319492
multiple acrosomal vesicles and their differentiation during spermiogenesis in ascidia zara and ascidia gemmata (ascidiacea, tunicata).a fully differentiated spermatozoon of both ascidia zara and ascidia gemmata is approximately 35 microm long. it contains a head and a tail lacking a midpiece. the head (approximately 4 microm long for a. zara and 5 microm long for a. gemmata) contains an elongated nucleus and a single mitochondrion that flanks the nucleus. multiple acrosomal vesicles (three or four in number) are present at the apex of the sperm head in both species. each vesicle is approximately 50 x 50 x 60 nm, and contains m ...199910521871
cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell.the organisation of cells of the planctomycete species pirellula marina, isosphaera pallida, gemmata obscuriglobus, planctomyces maris and "candidatus brocadia anammoxidans" was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of gemmata obscuriglobus and pirellula marina, computer-aided 3-d reconstructions from serial sections of cryosubstituted cells. all planctomycete cells display a peripheral ribosome-free re ...200111491082
16s-23s rdna intergenic spacer and 23s rdna of anaerobic ammonium-oxidizing bacteria: implications for phylogeny and in situ detection.recently, anaerobic ammonium-oxidizing bacteria (aaob) were identified by comparative 16s rdna sequence analysis as a novel, deep-branching lineage within the planctomycetales. this lineage consists currently of only two, not yet culturable bacteria which have been provisionally described as candidatus 'brocadia anammoxidans' and candidatus 'kuenenia stuttgartiensis'. in this study, a large fragment of the rdna operon, including the 16s rdna, the intergenic spacer region (isr) and approximately ...200111553235
membrane-bounded nucleoid in the eubacterium gemmatata obscuriglobus.the freshwater budding eubacterium gemmata obscuriglobus possesses a dna-containing nuclear region that is bounded by two nuclear membranes. the membrane-bounded nature of the nucleoid in this bacterium was shown by thin sectioning of chemically fixed cells, thin sectioning of freeze-substituted cells, and freeze-fracture/freeze-etch. the fibrillar nucleoid was surrounded by electron-dense granules that were in turn enveloped by two nuclear membranes separated by an electron-transparent space. i ...199111607213
isolation of gemmata-like and isosphaera-like planctomycete bacteria from soil and freshwater.new cultured strains of the planctomycete division (order planctomycetales) of the domain bacteria related to species in the genera gemmata and isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. phylogenetic analysis of the 16s rrna gene from eight representative isolates showed that all the isolates were members of the planctomycete division. six isolates clustered with gemmata obscuriglobus and related strains, while two isolates clustered with isosphaera pal ...200211772655
vanadocytes, cells hold the key to resolving the highly selective accumulation and reduction of vanadium in ascidians.since henze discovered vanadium in the blood (or coelomic) cells of an ascidian in 1911, this unusual phenomenon has attracted the interest of many investigators. the highest concentration of vanadium (350 mm) in the blood cells of ascidia gemmata, which belongs to the suborder phlebobranchia, is 10(7) times higher than that in seawater. of the approximately 10 types of blood cells, a combination of cell fractionation and neutron-activation analysis revealed that the signet ring cells were the t ...200211921344
gene discovery within the planctomycete division of the domain bacteria using sequence tags from genomic dna libraries.the planctomycetes comprise a distinct group of the domain bacteria, forming a separate division by phylogenetic analysis. the organization of their cells into membrane-defined compartments including membrane-bounded nucleoids, their budding reproduction and complete absence of peptidoglycan distinguish them from most other bacteria. a random sequencing approach was applied to the genomes of two planctomycete species, gemmata obscuriglobus and pirellula marina, to discover genes relevant to thei ...200212093378
molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant.we examined anoxic and aerobic basins and an anaerobic digestor of a municipal wastewater treatment plant for the presence of novel planctomycete-like diversity. three 16s rrna gene libraries were constructed by using a 16s rrna-targeted universal reverse primer and a forward pcr primer specific for planctomyces: phylogenetic analysis of 234 16s rrna gene sequences defined 110 operational taxonomic units. the majority of these sequences clustered with the four known genera, pirellula (32%), plan ...200314660385
phylogenetic and biochemical evidence for sterol synthesis in the bacterium gemmata obscuriglobus.sterol biosynthesis is viewed primarily as a eukaryotic process, and the frequency of its occurrence in bacteria has long been a subject of controversy. two enzymes, squalene monooxygenase and oxidosqualene cyclase, are the minimum necessary for initial biosynthesis of sterols from squalene. in this work, 19 protein gene sequences for eukaryotic squalene monooxygenase and 12 protein gene sequences for eukaryotic oxidosqualene cyclase were compared with all available complete and partial prokaryo ...200314660793
comparative analysis of ribonuclease p rna of the planctomycetes.the planctomycetes, order planctomycetales, are a distinct phylum of domain bacteria. genes encoding the rna portion of ribonuclease p (rnase p) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. rnpb gene sequences encoding rnase p rna were generated by a conserved primer pcr strategy for planctomyces brasiliensis, planctomyces limnophilus, pirellula marina, pirellula staleyi strain atcc 35122, isosphaera pallida, one other isosphaera strai ...200415280311
diversity and seasonal changes of uncultured planctomycetales in river biofilms.cell counts of planctomycetes showed that there were high levels of these organisms in the summer and low levels in the winter in biofilms grown in situ in two polluted rivers, the elbe river and the spittelwasser river. in this study 16s rrna-based methods were used to investigate if these changes were correlated with changes in the species composition. planctomycete-specific clone libraries of the 16s rrna genes found in both rivers showed that there were seven clusters, which were distantly r ...200415345387
eukaryotic signature proteins of prosthecobacter dejongeii and gemmata sp. wa-1 as revealed by in silico analysis.the genomes of representatives of three bacterial phyla have been compared with the list of 347 eukaryotic signature proteins (esps) derived by hartman and fedorov [proc. natl. acad. sci. usa 99 (2002) 1420]. the species included prosthecobacter dejongeii of the verrucomicrobia phylum, gemmata sp. wa-1 of the planctomycetes phylum and caulobacter crescentus of the proteobacteria. the protist trypanosoma brucei was used as a eukaryotic control. p. dejongeii had unique ergo blast matches to alpha- ...200515667994
archaea-like genes for c1-transfer enzymes in planctomycetes: phylogenetic implications of their unexpected presence in this phylum.the unexpected presence of archaea-like genes for tetrahydromethanopterin (h4mpt)-dependent enzymes in the completely sequence geiome of the aerobic marine planctomycete pirellula sp. strain 1 ("rhodopirellula baltica") and in the currently sequenced genome of the aerobic freshwater planctomycete gemmata obscuriglobus strain uqm2246 revives the discussion on the origin of these genes in the bacterial domain. we compared the genomic arrangement of these genes in planctomyetes and methylotrophic p ...200415693614
intracellular compartmentation in planctomycetes.the phylum planctomycetes of the domain bacteria consists of budding, peptidoglycan-less organisms important for understanding the origins of complex cell organization. their significance for cell biology lies in their possession of intracellular membrane compartmentation. all planctomycetes share a unique cell plan, in which the cell cytoplasm is divided into compartments by one or more membranes, including a major cell compartment containing the nucleoid. of special significance is gemmata obs ...200515910279
mtdc, a novel class of methylene tetrahydromethanopterin dehydrogenases.novel methylene tetrahydromethanopterin (h4mpt) dehydrogenase enzymes, named mtdc, were purified after expressing in escherichia coli genes from, respectively, gemmata sp. strain wa1-1 and environmental dna originating from unidentified microbial species. the mtdc enzymes were shown to possess high affinities for methylene-h4mpt and nadp but low affinities for methylene tetrahydrofolate or nad. the substrate range and the kinetic properties revealed by mtdc enzymes distinguish them from the prev ...200516109948
[detection of representatives of the planctomycetes in sphagnum peat bogs by molecular and cultivation methods].by means of fluorescence in situ hybridization with 16s rrna-targeted oligonucleotide probes (fish), it has been shown that members of the phylum planctomycetes represent a numerically significant bacterial group in boreal sphagnum peat bogs. the population size of planctomycetes in oxic layers of the peat bog profile was in the range of 0.4-2.0 x 10(7) cells per g of wet peat, comprising 4 to 13% of the total bacterial cell number. a novel effective approach that combined a traditional cultivat ...200616871807
the last eukaryotic common ancestor (leca): acquisition of cytoskeletal motility from aerotolerant spirochetes in the proterozoic eon.we develop a symbiogenetic concept of the origin of eukaryotic intracellular motility systems from anaerobic but aerotolerant spirochetes in sulfide-rich environments. the last eukaryotic common ancestors (lecas) have extant archaeprotist descendants: motile nucleated cells with embden-meyerhof glycolysis and substrate-level phosphorylation that lack the alpha-proteobacterial symbiont that became the mitochondrion. swimming and regulated o(2)-tolerance via sulfide oxidation already had been acqu ...200616938841
whole-genome reciprocal blast analysis reveals that planctomycetes do not share an unusually large number of genes with eukarya and archaea.the genome sequences of rhodopirellula baltica, formerly pirellula sp. strain 1, blastopirellula marina, gemmata obscuriglobus, and kuenenia stuttgartiensis were used in a series of pairwise reciprocal best-hit analyses to evaluate the contested evolutionary position of planctomycetes. contrary to previous reports which suggested that r. baltica had a high percentage of genes with closest matches to archaea and eukarya, we show here that these planctomycetes do not share an unusually large numbe ...200617021241
[high abundance of planctomycetes in anoxic layers of a sphagnum peat bog].the depth distribution of planctomycete abundance has been examined in six different sites of the sphagnum peat bog in bakchar, tomsk oblast, russia. in situ hybridization of peat with the fluorescently labeled oligonucleotide probes pla46 and pla886, reported to be group-specific for representatives of the phylum planctomycetes, revealed two distinct population maxima of these bacteria in all of the profiles examined. the first population maximum was detected in the uppermost, oxic layer of the ...200617205808
close relationship of rnase p rna in gemmata and anammox planctomycete bacteria.the relationship of rnase p rna from anammox bacteria 'candidatus brocadia anammoxidans' and 'candidatus kuenenia stuttgartiensis' with that from other planctomycetes was investigated. newly identified rnpb gene sequences were aligned against existing planctomycete rnase p rna sequences and secondary structures deduced by a comparative approach. deduced secondary structures were similar in both anammox bacteria and both possessed an insert within the p13 helix analogous to that present in all ge ...200717328750
on the classification, evolution and biogeography of terrestrial haemadipsoid leeches (hirudinida: arhynchobdellida: hirudiniformes).a scourge of tropical and subtropical jungles, bloodfeeding terrestrial leeches of haemadipsidae have long confused systematists and defied sensible biogeographic interpretation. the family haemadipsidae usually includes problematic taxa that neither fit the typical indopacific distribution of the group, nor properly match diagnostic characters used to define the family. historically, four additional families-xerobdellidae, diestecostomatidae mesobdellidae and nesophilaemonidae-have occasionally ...200817977750
fitting together: copulatory linking in some neotropical chrysomeloidea.copulatory linking of male and female genitalic structures in 11 neotropical species of chrysomelidae and one species of megalopodidae was studied by freezing and then dissecting pairs of beetles in copula. in megalopus armatus (megalopodidae) the male has a long endophallus with complex membranous protuberances and a terminal flagellum that probably reaches the spermatheca. in the subfamily eumolpinae the females have telescoping ovipositors through which the male endophalli pass, reaching to o ...200618491623
different planctomycetes diversity patterns in latitudinal surface seawater of the open sea and in sediment.the 16s rrna gene approach was applied to investigate the diversity of planctomycetes in latitudinal surface seawater of the western pacific ocean. the results revealed that the pirellula-rhodopirellula-blastopirellula clade dominated the planctomycetes community at all surface seawater sites while the minority genera gemmata and planctomyces were only found at sites h5 and h2 respectively. although the clone frequency of the prb clade seemed stable (between 83.3% and 94.1%) for all surface seaw ...200818545964
characterization of juvenile maritime pine (pinus pinaster ait.) ectomycorrhizal fungal community using morphotyping, direct sequencing and fruitbodies sampling.using ectomycorrhizal root tip morphotyping (anatomical and morphological identification), molecular analysis (internal transcribed spacer region amplification and sequencing), and fruitbody sampling, we assessed diversity and composition of the ectomycorrhizal fungal community colonizing juvenile pinus pinaster ait. under natural conditions in nw spain. overall, we found 15 basidiomycetes and two ascomycetes. members of the family thelephoraceae represented up to 59.4% of the samples. the most ...200918972139
chromatin organization and radio resistance in the bacterium gemmata obscuriglobus.the organization of chromatin has a major impact on cellular activities, such as gene expression. for bacteria, it was suggested that the spatial organization of the genetic material correlates with transcriptional levels, implying a specific architecture of the chromosome within the cytoplasm. accordingly, recent technological advances have emphasized the organization of the genetic material within nucleoid structures. gemmata obscuriglobus, a member of the phylum planctomycetes, exhibits a dis ...200919074379
the cell cycle of the planctomycete gemmata obscuriglobus with respect to cell compartmentalization.gemmata obscuriglobus is a distinctive member of the divergent phylum planctomycetes, all known members of which are peptidoglycan-less bacteria with a shared compartmentalized cell structure and divide by a budding process. g. obscuriglobus in addition shares the unique feature that its nucleoid dna is surrounded by an envelope consisting of two membranes forming an analogous structure to the membrane-bounded nucleoid of eukaryotes and therefore g. obscuriglobus forms a special model for cell b ...200919144151
zavarzinella formosa gen. nov., sp. nov., a novel stalked, gemmata-like planctomycete from a siberian peat bog.an aerobic, pink-pigmented, budding and rosette-forming bacterium was isolated from an acidic sphagnum peat bog and designated strain a10(t). the 16s rrna gene sequence analysis showed that strain a10(t) was a member of the order planctomycetales and belonged to a phylogenetic lineage defined by the genus gemmata, with 90 % sequence similarity to that of gemmata obscuriglobus, the only taxonomically described organism of this group. ellipsoid-shaped cells of strain a10(t) were uniformly covered ...200919196778
phylogenetic analysis of the triterpene cyclase protein family in prokaryotes and eukaryotes suggests bidirectional lateral gene transfer.functional constraints to modifications in triterpene cyclase amino acid sequences make them good candidates for evolutionary studies on the phylogenetic relatedness of these enzymes in prokaryotes as well as in eukaryotes. in this study, we used a set of identified triterpene cyclases, a group of mainly bacterial squalene cyclases and a group of predominantly eukaryotic oxidosqualene cyclases, as seed sequences to identify 5288 putative triterpene cyclase homologues in publicly available databa ...200919207562
gene cloning and characterization of a novel esterase from activated sludge metagenome.a metagenomic library was prepared using pcc2fos vector containing about 3.0 gbp of community dna from the microbial assemblage of activated sludge. screening of a part of the un-amplified library resulted in the finding of 1 unique lipolytic clone capable of hydrolyzing tributyrin, in which an esterase gene was identified. this esterase/lipase gene consists of 834 bp and encodes a polypeptide (designated estas) of 277 amino acid residuals with a molecular mass of 31 kda. sequence analysis indic ...200920028524
the compartmentalized bacteria of the planctomycetes-verrucomicrobia-chlamydiae superphylum have membrane coat-like proteins.the development of the endomembrane system was a major step in eukaryotic evolution. membrane coats, which exhibit a unique arrangement of beta-propeller and alpha-helical repeat domains, play key roles in shaping eukaryotic membranes. such proteins are likely to have been present in the ancestral eukaryote but cannot be detected in prokaryotes using sequence-only searches. we have used a structure-based detection protocol to search all proteomes for proteins with this domain architecture. apart ...201020087413
endocytosis-like protein uptake in the bacterium gemmata obscuriglobus.endocytosis is a process by which extracellular material such as macromolecules can be incorporated into cells via a membrane-trafficking system. although universal among eukaryotes, endocytosis has not been identified in bacteria or archaea. however, intracellular membranes are known to compartmentalize cells of bacteria in the phylum planctomycetes, suggesting the potential for endocytosis and membrane trafficking in members of this phylum. here we show that cells of the planctomycete gemmata ...201020566852
broad-spectrum antibiotic resistance of planctomycetes organisms determined by etest.the in vitro susceptibility of planctomycetes organisms to antibiotics has seldom been studied and when it has, a variety of methods have been used. the objective of the study was to expand the knowledge of planctomycetes antibiotic susceptibility patterns.201020699245
facing unknowns: living cultures (pirum gemmata gen. nov., sp. nov., and abeoforma whisleri, gen. nov., sp. nov.) from invertebrate digestive tracts represent an undescribed clade within the unicellular opisthokont lineage ichthyosporea (mesomycetozoea).during a culture-based survey of opisthokonts living in marine invertebrate digestive tracts, we isolated two new eukaryotes that differed from described taxa by more than 10% in their small subunit ribosomal dna sequences. phylogenetic analysis showed that the two isolates represented a divergent clade of ichthyosporeans known previously only from environmental clone sequences. we used light and electron microscopy to describe the isolates as new genera and species pirum gemmata and abeoforma w ...201120708961
comparative anatomy of the nectary spur in selected species of aeridinae (orchidaceae).to date, the structure of the nectary spur of aeridinae has not been studied in detail, and data relating to the nectaries of ornithophilous orchids remain scarce. the present paper compares the structural organization of the floral nectary in a range of aeridinae species, including both entomophilous and ornithophilous taxa.201021183455
protein uptake by bacteria: an endocytosis-like process in the planctomycete gemmata obscuriglobus.endocytosis is a fundamental process of membrane-trafficking in eukaryotes, but has not been known to occur in bacteria or archaea. the origin of endocytosis is central to the understanding of evolution of the first eukaryotes and their endomembrane systems. in a recent study we have established that an endocytosis-like process for uptake of proteins into cells occurs in a bacterium, gemmata obscuriglobus, a member of the distinctive phylum planctomycetes of peptidoglycan-less budding bacteria. ...201021331243
making heads or tails of the hu-proteins in the planctomycete gemmata obscuriglobus.gemmata obscuriglobus has a highly condensed nucleoid which is implicated in its resistance to radiation. however, the mechanisms by which such compaction is achieved, or the proteins responsible involved are still unknown. here we have examined the genome of g.obscuriglobus for presence of proteins homologous to those that have been associated with nucleoid condensation. we found two different proteins homologous to the bacterial nucleoid-associated protein hu, one with an n-terminal and one wi ...201121511768
beyond the bacterium: planctomycetes challenge our concepts of microbial structure and function.planctomycetes form a distinct phylum of the domain bacteria and possess unusual features such as intracellular compartmentalization and a lack of peptidoglycan in their cell walls. remarkably, cells of the genus gemmata even contain a membrane-bound nucleoid analogous to the eukaryotic nucleus. moreover, the so-called 'anammox' planctomycetes have a unique anaerobic, autotrophic metabolism that includes the ability to oxidize ammonium; this process is dependent on a characteristic membrane-boun ...201121572457
identification of a novel vanadium-binding protein by est analysis on the most vanadium-rich ascidian, ascidia gemmata.ascidians are known to accumulate extremely high levels of vanadium in their blood cells (up to 350 mm). the branchial sac and the intestine are thought to be the first tissues to contact the outer environment and absorb vanadium ions. the concentration of vanadium in the branchial sac and the intestine of the most vanadium-rich ascidian ascidia gemmata were determined to be 32.4 and 11.9 mm, respectively. using an expressed sequence tag (est) analysis of a cdna library from the intestine of a. ...201121748343
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