| [blastobacter viscosus, a new species of methanol-utilizing autotrophic bacteria]. | a strain of budding bacteria capable of autotrophic growth in the atmosphere of h2+o2+co2 or in a medium with methanol was isolated from activated sludge. the cells are gram negative nonmotile yellow pleomorphic rods which do not form rosettes. the bacterium is a strict aerobe. it utilizes a wide range of organic compounds (alcohols, sugars and organic acids) as the sources of carbon and energy. the g+c content of dna is 66.3 +/- 0.9 mol %. based on the study of morphological, cultural, and phys ... | 1979 | 502906 |
| evidence for a close genealogical relationship between afipia (the causal organism of cat scratch disease), bradyrhizobium japonicum and blastobacter denitrificans. | the primary structures of the small subunit rrna of bradyrhizobium japonicum and blastobacter denitrificans were determined by direct sequencing of enzymatically amplified dna. comparative sequence analysis revealed that bradyrhizobium japonicum and blastobacter denitrificans are members of the alpha-2 subgroup of the proteobacteria and show a very close phylogenetic relationship with the genus afipia. | 1992 | 1383084 |
| heterotrophic bacteria in an air-handling system. | heterotrophic bacteria from structural surfaces, drain pan water, and the airstream of a well-maintained air-handling system with no reported building-related illness were enumerated. visually the system appeared clean, but large populations of bacteria were found on the fin surface of the supply-side cooling coils (10(5) to 10(6) cfu cm-2), in drain pan water (10(5) to 10(7) cfu ml-1), and in the sump water of the evaporative condenser (10(5) cfu ml-1). representative bacterial colony types rec ... | 1992 | 1476435 |
| budding bacteria of the pasteuria-blastobacter group. | | 1973 | 4122691 |
| two unusual budding bacteria isolated from a swimming pool. | two unusual strains of budding bacteria were isolated on a millipore pseudomonas count water tester during routine monitoring of pseudomonas aeruginosa counts in a swimming pool. the first isolate has been identified as blastobacter sp. it was a yellow-pigmented, gram negative rod-shaped organism with a polar holdfast by which it attached to solid surfaces or other cells to form rosettes. the cells reproduced by asymmetric division or budding at the free pole of the cell, producing motile daught ... | 1984 | 6746465 |
| phenotypic and genotypic characterization of bradyrhizobia nodulating the leguminous tree acacia albida. | rhizobial isolates that were obtained from both surface and deep soil samples in the sahelian and sudano-guinean areas of senegal (west africa) under acacia albida trees were compared with representative strains of known rhizobial species and genera. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of proteins was used to determine the taxonomic positions of these organisms and the relationships between isolates obtained from the surface and isolates obtained from deep soil. ... | 1994 | 7520737 |
| genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots. | seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ... | 1995 | 7538280 |
| thermostable n-carbamoyl-d-amino acid amidohydrolase: screening, purification and characterization. | a thermostable n-carbamoyl-d-amino acid amidohydrolase was found in the cells of newly isolated bacterium. blastobacter sp. a17p-4. the bacterium also showed d-specific hydantoinase activity. the n-carbamoyl-d-amino acid amidohydrolase activity of the cells exhibited a temperature optimum at 50-55 degrees c, and was stable up to 50 degrees c. the n-carbamoyl-d-amino acid amidohydrolase of blastobacter sp. a17p-4 was purified to homogeneity and characterized. it has a relative molecular weight of ... | 1994 | 7765578 |
| taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of sphingomonas aromaticivorans sp. nov., sphingomonas subterranea sp. nov., and sphingomonas stygia sp. nov. | phylogenetic analyses of 16s rrna gene sequences by distance matrix and parsimony methods indicated that six strains of bacteria isolated from deep saturated atlantic coastal plain sediments were closely related to the genus sphingomonas. five of the strains clustered with, but were distinct from, sphingomonas capsulata, whereas the sixth strain was most closely related to blastobacter natatorius. the five strains that clustered with s. capsulata, all of which could degrade aromatic compounds, w ... | 1997 | 8995822 |
| imidase, a dihydropyrimidinase-like enzyme involved in the metabolism of cyclic imides. | imidase, which preferably hydrolyzed cyclic imides to monoamidated dicarboxylates, was purified to homogeneity from a cell-free extract of blastobacter sp. a17p-4. cyclic imides are known to be hydrolyzed by mammalian dihydropyrimidinases. however, imidase was quite different from known dihydropyrimidinases in structure and substrate specificity. the enzyme has a relative molecular mass of 105 000 and consists of three identical subunits. the purified enzyme showed higher activity and affinity t ... | 1997 | 9030755 |
| transfer of blastobacter natatorius (sly 1985) to the genus blastomonas gen. nov. as blastomonas natatoria comb. nov. | the budding bacterium blastobacter natatorius belongs to the alpha-4 group of the proteobacteria and clusters phylogenetically on a deep branch with sphingomonas capsulata, with which it shares 93.9% 16s rrna sequence similarity. on phylogenetic, phenotypic, and chemotaxonomic grounds a proposal is made to transfer b. natatorius to the genus blastomonas gen. nov. as blastomonas natatoria comb. nov. | 1997 | 9103650 |
| phylogeny and identification in situ of nevskia ramosa. | an enrichment of the neuston bacterium nevskia ramosa was investigated by the cultivation-independent rrna approach. n. ramosa was first described by famintzin in 1892 as a rod-shaped, slightly bent bacterium forming typical flat rosettes on the surface of shallow freshwater habitats by unilateral slime formation. pcr in combination with cloning and sequencing was used for retrieving 21 partial and 5 nearly full-length 16s rrna sequences forming three tight clusters. in situ hybridization with r ... | 1998 | 9572969 |
| development of pcr primer systems for amplification of nitrite reductase genes (nirk and nirs) to detect denitrifying bacteria in environmental samples. | a system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with pcr. primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirk and nirs) after comparative sequence analysis. whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. likewise, the method allowed a determination of the nir type of five laboratory ... | 1998 | 9758798 |
| b-90063, a novel endothelin converting enzyme inhibitor isolated from a new marine bacterium, blastobacter sp. sank 71894. | a novel endothelin-converting enzyme (ece) inhibitor, b-90063, was isolated from the culture supernatant of the newly discovered marine bacterium blastobacter sp. sank 71894. based on spectral analyses and chemical reactions, the structure of b-90063 was determined to be bis[6-formyl-4-hydroxy-2-(2'-n-pentyloxazol-4'-yl)-4-pyridon -3-yl]-disulfide (1a). human and rat eces were inhibited more potently by b-90063, with respective ic50 values of 1.0 and 3.2 microm, than were other neutral endopepti ... | 1998 | 9820230 |
| cyclic-imide-hydrolyzing activity of d-hydantoinase from blastobacter sp. strain a17p-4. | the cyclic-imide-hydrolyzing activity of a prokaryotic cyclic-ureide-hydrolyzing enzyme, d-hydantoinase, was investigated. the enzyme hydrolyzed cyclic imides with bulky substituents such as 2-methylsuccinimide, 2-phenylsuccinimide, phthalimide, and 3,4-pyridine dicarboximide to the corresponding half-amides. however, simple cyclic imides without substituents, which are substrates of imidase (ie.g., succinimide, glutarimide, and sulfur-containing cyclic imides such as 2,4-thiazolidinedione and r ... | 1999 | 10515797 |
| a novel amidase (half-amidase) for half-amide hydrolysis involved in the bacterial metabolism of cyclic imides. | a novel amidase involved in bacterial cyclic imide metabolism was purified from blastobacter sp. strain a17p-4. the enzyme physiologically functions in the second step of cyclic imide degradation, i.e., the hydrolysis of monoamidated dicarboxylates (half-amides) to dicarboxylates and ammonia. enzyme production was enhanced by cyclic imides such as succinimide and glutarimide but not by amide compounds which are conventional substrates and inducers of known amidases. the purified amidase showed h ... | 2000 | 10788365 |
| photosynthetic bradyrhizobia are natural endophytes of the african wild rice oryza breviligulata. | we investigated the presence of endophytic rhizobia within the roots of the wetland wild rice oryza breviligulata, which is the ancestor of the african cultivated rice oryza glaberrima. this primitive rice species grows in the same wetland sites as aeschynomene sensitiva, an aquatic stem-nodulated legume associated with photosynthetic strains of bradyrhizobium. twenty endophytic and aquatic isolates were obtained at three different sites in west africa (senegal and guinea) from nodal roots of o. ... | 2000 | 11097925 |
| phylogenetic and dna-dna hybridization analyses of bradyrhizobium species. | the 16s rdna sequence of bradyrhizobium liaoningense was determined and analysed together with sequences of other bradyrhizobium species and related taxa. in addition, dna-dna hybridizations were performed between representative strains of the three bradyrhizobium species. bradyrhizobium liaoningense is genotypically highly related to bradyrhizobium japonicum, whereas bradyrhizobium elkanii is more distantly related to these two species. the fact that afipia, agromonas, blastobacter, nitrobacter ... | 2001 | 11211247 |
| rapid ribosequencing--an effective diagnostic tool for detecting microbial infection. | rapid and reliable identification of microorganisms is a prerequisite for the diagnosis and subsequent treatment of infectious diseases. the identification of pathogenic bacteria is traditionally based on their isolation from clinical samples and propagation on culture medium in the routine laboratory. however, despite clinical signs of infection, culture of the pathogenic agent often fails. this may be due to a low number of microorganisms, prior antibiotic treatment, nonculturable microorganis ... | 2001 | 11261751 |
| albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane. | a novel genus, albibacter, with one species, albibacter methylovorans sp. nov., is proposed for a facultatively chemolithotrophic and methylotrophic bacterium (strain dm10t) with the ribulose bisphosphate (rubp) pathway of c1 assimilation. the bacterium is a gram-negative, aerobic, asporogenous, nonmotile, colourless rod that multiplies by binary fission. the organism utilizes dichloromethane, methanol, methylamine, formate and co2/h2, as well as a variety of polycarbon compounds, as carbon and ... | 2001 | 11411673 |
| the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica. | blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ... | 2002 | 11872460 |
| disseminated infection due to blastobacter denitrificans following routine appendectomy in an adolescent. | until now, blastobacter denitrificans has not been mentioned in the context of human infections. a case of severe complication caused by b. denitrificans after routine appendectomy in a young girl is described and confirms this organism to be an opportunistic human pathogen. | 2002 | 12202615 |
| emendation of the genus sphingomonas yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, sphingobium, novosphingobium and sphingopyxis, in conjunction with blastomonas ursincola. | the 16s rdna sequence similarities between the type strains of sphingomonas paucimobilis and 32 other sphingomonas species range from 90.2 to 99.6%. it might be possible to divide the genus into several new genera according to a dendrogram drawn from 16s rdna sequence similarity. however, the phenotypic and biochemical information needed to define clusters of strains representing distinct genera within this group of organisms was not previously available. although the cellular lipids of type str ... | 2002 | 12361250 |
| [cloming, sequence analysis of imidase gene from alcaligenes eatrophus and its expression in e. coli]. | a hydantoin-cleaving microorganism 112r4 is screened and identified to be alcaligenes eutrophus. the resting cell of alcaligenes eutrophus 112r4 can catalyze the hydrolysis of hydantoin, dihydropyrimidine and succinimide effectively, but not function to 5-monosubstituted hydantoins or 5,5'-disubstituted hydantoins. the microorganism can utilize succinimide as a sole carbon source and nitrogen source, which indicates the presence of a complete transformation pathway of succinimide, and a hydantoi ... | 2002 | 12557390 |
| phenotypic and genotypic diversity of rhizobia nodulating pterocarpus erinaceus and p. lucens in senegal. | a total of fifty root nodules isolates of fast-growing and slow growing rhizobia from pterocarpus ennaceus and pterocarpus lucens respectively native of sudanean and sahelian regions of senegal were characterized. these isolates were compared to representative strains of known rhizobial species. twenty-two new isolates were slow growers and twenty-eight were fast growers. a polyphasic approach was performed including comparative total protein sodium dodecyl sulphate polyacrylamide gel (sds-page) ... | 2002 | 12583718 |
| reclassification of 'blastobacter viscosus' 7d and 'blastobacter aminooxidans' 14a as xanthobacter viscosus sp. nov. and xanthobacter aminoxidans sp. nov. | on the basis of morphological, physiological and genotypic properties 'blastobacter viscosus' 7d and 'blastobacter aminooxidans' 14a are proposed as new species of the genus xanthobacter, xanthobacter viscosus (type strain 7dt =vkm b-2253t =atcc baa-298t) and xanthobacter aminoxidans corrig. (type strain 14at =vkm b-2254t =atcc baa-299t). | 2003 | 12656170 |
| molecular diversity of denitrifying genes in continental margin sediments within the oxygen-deficient zone off the pacific coast of mexico. | to understand the composition and structure of denitrifying communities in the oxygen-deficient zone off the pacific coast of mexico, the molecular diversity of nir genes from sediments obtained at four stations was examined by using a pcr-based cloning approach. a total of 50 operational taxonomic units (otus) for nirk and 82 otus for nirs were obtained from all samples. forty-four of the nirs clones and 31 of the nirk clones were sequenced; the levels of similarity of the nirs clones were 52 t ... | 2003 | 12788762 |
| changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. | as of february 2003, bacteria that form nitrogen-fixing symbiotic associations with legumes have been confirmed in 44 species of 12 genera. phylogenies of these taxa containing legume symbionts based on the comparative analysis of 16s rdna sequences show that they are not clustered in one lineage but are distributed in the classes alphaproteobacteria and betaproteobacteria, and dispersed over the following nine monophyletic groups, being intermingled with other taxa that do not contain legume sy ... | 2003 | 12949698 |
| bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. | a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ... | 2003 | 13130002 |
| purification and characterization of carbaryl hydrolase from blastobacter sp. strain m501. | a bacterium capable of hydrolyzing carbaryl (1-naphthyl-n-methylcarbamate) was isolated from a soil enrichment. this bacterium was characterized taxonomically as a blastobacter sp. and designated strain m501. a carbaryl hydrolase present in this strain was purified to homogeneity by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic, anion-exchange, gel filtration, and hydroxylapatite chromatographies. the native enzyme had a molecular mass of 166,000 da and was compose ... | 1993 | 16348989 |
| phylogenetic analysis of bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from aeschynomene species grown in separated geographical regions. | nearly complete and short partial 16s rrna sequences were derived from pcr-amplified ribosomal dnas of bradyrhizobium japonicum usda 136 and usda 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of aeschynomene indica and other aeschynomene species growing in different geographic regions, including india, the philippines and north america. we confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption s ... | 1994 | 16349221 |
| novel metabolic transformation pathway for cyclic imides in blastobacter sp. strain a17p-4. | the metabolic transformation pathway for cyclic imides in microorganisms was studied in blastobacter sp. strain a17p-4. this novel pathway involves, in turn, hydrolytic ring opening of a cyclic imide to yield a monoamidated dicarboxylate, hydrolytic deamidation of the monoamidated dicarboxylate to yield a dicarboxylate, and dicarboxylate transformation similar to that in the tricarboxylic acid cycle. the initial step is catalyzed by a novel enzyme, imidase. imidase and subsequent enzymes involve ... | 1996 | 16535426 |
| proposal for combining bradyrhizobium spp. (aeschynomene indica) with blastobacter denitrificans and to transfer blastobacter denitrificans (hirsch and muller, 1985) to the genus bradyrhizobium as bradyrhizobium denitrificans (comb. nov.). | the symbiotic bradyrhizobia of aeschynomene indica and the aquatic budding bacterium blastobacter denitrificans have much in common and this study broadens the characters that are shared between the two. the 23s rrna gene sequences of the bradyrhizobial isolates were most similar to each other and to the sequence of bl. denitrificans. evidence for the presence of photosynthetic genes in the genome of bl. denitrificans was obtained by pcr using primers to the conserved m subunit (pufm) of the pho ... | 2006 | 16564957 |
| gene cloning, expression, and substrate specificity of an imidase from the strain pseudomonas putida yz-26. | a gene-encoding imidase was isolated from pseudomonas putdia yz-26 genomic dna using a combination of polymerase chain reaction and activity screening the recombinant. analysis of the nucleotide sequence revealed that an open reading frame (orf) of 879 bp encoded a protein of 293 amino acids with a calculated molecular weight of 33712.6 kda. the deduced amino-acid sequence showed 78% identity with the imidase from alcaligenes eutrophus 112r4 and 80% identity with n-terminal 20 amino-acid imidase ... | 2007 | 17534563 |
| [beta-proteorhizobia and nonrhizobial species--a review]. | the terms alpha- and beta-rhizobia were proposed to distinguish the rhizobial alpha- and beta-proteorhizobia respectively. in this review we present recently development of rhizobial taxonomy about beta-rhizobia in the genus burkholeria, cupriavidus isolated from mimosa and alpha-proteorhizobia belonging to methylobacterium, devosia, ochrobactrum, phyllobacterium and blastobacter lineage. furthermore, we also introduced the evolution of symbiosis related genes such as noda and nifh, and posed so ... | 2008 | 19160827 |
| microbially influenced corrosion of galvanized steel pipes in aerobic water systems. | to investigate the role of heterotrophic bacteria in the corrosion of galvanized steel in the presence of water. | 2010 | 20070443 |
| rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of blastobacter aggregatus hirsch and muller 1986 as rhizobium aggregatum comb. nov. | a gram-negative, rod-shaped, motile, aerobic bacterial strain, w3(t), was isolated from hexachlorocyclohexane (hch)-contaminated groundwater from lucknow, india, and its taxonomic position was determined using a polyphasic approach. strain w3(t) shared highest 16s rrna gene sequence similarity of 97.8 % with rhizobium selenitireducens b1(t), followed by rhizobium daejeonense l61(t) (97.7 %), rhizobium radiobacter atcc 19358(t) (97.5 %) and blastobacter aggregatus ifam 1003(t) (97.2 %). strain w3 ... | 2011 | 20584817 |