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isolation and characterization of gas vesicles from microcyclus aquaticus.intact gas vesicles of microcyclus aquaticus s1 were isolated by using centrifugally accelerated flotation of vesicles and molecular sieve chromatography. isolated gas vesicles were cylindrical organelles with biconical ends and measured 250x100nm. the gas vesicle membrane was composed almost entirely of protein; neither lipid nor carbohydrate was detected, although one mole of phosphate per mole of protein was found. amino acid analysis indicated that the protein contained 54.6% hydrophobic ami ...1977403898
microcyclus and related ring-forming bacteria. 1977407051
[autotrophic methanol metabolism in microcyclus aquaticus].metabolism of methanol was studied in microcyclus aquaticus z-238 in enzymological experiments. this facultative methylotroph was found to assimilate the carbon of methanol after oxidation to co2 via the reductive pentose phosphate cycle. dehydrogenases of methanol, formaldehyde and formate as well as ribulose-1,5-diphosphate carboxylase were induced during the methylotrophic growth of the bacterium. the serine pathway and the hexulose phosphate cycle were not involved in methanol metabolism owi ...1978651686
[autotrophic growth of microcyclus aquaticus in hydrogen].microcyclus aquaticus z-238 which had been isolated as a methanol oxidizing culture was found to be capable of growth in the atmosphere of hydrogen, oxygen and carbon dioxide in the presence of yeast extract. autotrophic growth of the culture in the atmosphere of hydrogen required biotin or casaminic acids.1978672676
gas vesicle assembly in microcyclus aquaticus.when observed in the electron microscope intact gas vesicles appeared as transparent areas in whole cells of microcylus aquaticus, whereas vesicles collapsed by centrifugation were not discernible. within 5 min of suspending cells containing collapsed vesicles in growth medium, small transparent vesicles were detected. by 15 min the average number of vesicles per cell was 15. this number remained relatively constant while the size of the vesicles increased until they attained their maximum diamt ...1975807555
inhibition of gas vesicle production in microcyclus aquaticus by l-lysine.the timing and degree of gas vesicle production in microcyclus aquaticus was affected by nutritional conditions. if 50 microng l-lysine/ml was added to a glucose-mineral salts medium (dm), the organism did not form gas vesicles. this effect was specific for l-lysine, as neither d-lysine nor meso-diaminopimelic acid prevented gas vesicle production. cells grown in the presence of l-lysine did not contain any immunologically detectable gas vesicle protein, which indicates that l-lysine affects exp ...1977861844
degradation of 1,2-dichloroethane by ancylobacter aquaticus and other facultative methylotrophs.cultures of the newly isolated bacterial strains ad20, ad25, and ad27, identified as strains of ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (dce) as the sole carbon and energy source. these strains, as well as two other new dce utilizers, were facultative methylotrophs and were also able to grow on 2-chloroethanol, chloroacetate, and 2-chloropropionate. in all strains tested, dce was degraded by initial hydrolytic dehalogenation to 2-chloroethanol, followed by oxidation ...19921575500
family spirosomaceae: gram-negative ring-forming aerobic bacteria.the bacteria having a unique ring-like morphology first isolated from nasal mucus by weibel in 1887 were classified as a new genus spirosoma by migula in 1894. however, because these bacteria were not completely described for taxonomic purposes and their cultures were no longer available, the genus was deleted from the bergey's manual of determinative bacteriology, 6th edition, 1948. orskov (1928) created a new genus "microcyclus" (a name that has been found to be illegitimate and replaced with ...19902248690
oligotrophic methylotrophs: ancylobacter (basonym "microcyclus" orskov) raj gen. nov. 19892692603
lack of bubble formation in hypobarically decompressed cells.suspensions of human erythrocytes or of unicellular microorganisms (tetrahymena pyriformis, euglena gracilis, escherichia coli, and microcyclus aquaticus) were equilibrated with nitrogen gas pressures up to 200 atm and rapidly decompressed to hypobaric pressures below the vapor point of water. the intracellular environments proved to be very tolerant to the gas supersaturations induced. none or only a few cells were damaged in each case, and bubbles were never observed intracellularly after deco ...19873115244
ring-forming, oligotrophic microcyclus organisms in the water and mud of lake balaton.seventeen oligotrophic microcyclus strains were isolated from water- and mud-samples obtained in the region of the keszthely-bay (lake balaton, hungary) using different synthetic media with 1% na2s2o3. fifteen strains occurred in samples collected in the winter period. for numerical analysis, all isolates were compared with four authentic strains of recognized microcyclus spp. on the basis of 88 selected tests. they proved to be typical members of the species microcyclus aquaticus (orskov), cons ...19883227797
turgor pressure responses of a gram-negative bacterium to antibiotic treatment, measured by collapse of gas vesicles.the internal hydrostatic pressure of ancylobacter aquaticus was measured by collapsing the gas vesicles with an externally applied pressure. turgor pressure was measured in conjunction with various antibiotic treatments to elucidate some aspects of the biophysics of gram-negative cell wall function. differences in the effects of these drugs either alone or in combination with other treatments were related to known biochemical activities of these drugs. our previous work, demonstrating a heteroge ...19883343215
nephelometric determination of turgor pressure in growing gram-negative bacteria.gas vesicles were used as probes to measure turgor pressure in ancylobacter aquaticus. the externally applied pressure required to collapse the vesicles in turgid cells was compared with that in cells whose turgor had been partially or totally removed by adding an impermeable solute to the external medium. since gram-negative bacteria do not have rigid cell walls, plasmolysis is not expected to occur in the same way as it does in the cells of higher plants. bacterial cells shrink considerably be ...19873611024
variability of the turgor pressure of individual cells of the gram-negative heterotroph ancylobacter aquaticus.cells of ancylobacter aquaticus were observed under phase microscopy in a chamber to which a measured pressure could be applied. the initial collapse pressure (ca), i.e., the lowest pressure needed to collapse the most pressure-sensitive gas vesicles, was measured for 69 cells. the cells were taken from cultures in low-density balanced exponential growth, and the experiments were performed quickly so that the bacteria were in a uniform physiological state at the time of measurement. the turgor p ...19873654582
pathways of carbohydrate metabolism in microcyclus species.radiorespirometric and enzymatic studies were conducted to determine primary and secondary pathways of carbohydrate catabolism in microcyclus aquaticus and m. flavus. m. aquaticus catabolizes both glucose and gluconate mainly via the entner-doudoroff and pentose phosphate pathways with some concurrent participation of the embden-meyerhof pathway. m. flavus, however, oxidizes glucose mainly via the embden-meyerhof pathway and gluconate via the entner-doudoroff pathway with some simultaneous opera ...19734688142
[growth of a microcyclus culture on methanol]. 19734782425
gas-vacuolated strains of microcyclus aquaticus.two strains of gas-vacuolated heterotrophic bacteria have been isolated from freshwaters by using 0.01% peptone enrichments. both are nonmotile, vibrioid organisms that form rings before division. in terms of these morphological traits and other characteristics, including nutritional characters and deoxyribonucleic acid base composition, these new isolates are very similar to microcyclus aquaticus. it is proposed that they be considered strains of this species.19714941556
[new soil microorganism--renobacter vaculatum gen. et sp. n]. 19715562880
dna base composition of microcyclus species and organisms of similar morphology. 19685709639
microbial growth associated with granular activated carbon in a pilot water treatment facility.the microbial dynamics associated with granular activated carbon (gac) in a pilot water treatment plant were investigated over a period of 16 months. microbial populations were monitored in the influent and effluent waters and on the gac particles by means of total plate counts and atp assays. microbial populations between the influent and effluent waters of the gac columns generally increased, indicating microbial growth. the dominant genera of microorganisms isolated from interstitial waters a ...19836625567
rupture of the cell envelope by induced intracellular gas phase expansion in gas vacuolate bacteria.using a new approach, we estimated the physical strength of the cell envelopes of three species of gram-negative, gas vacuolate bacteria (microcyclus aquaticus, prosthecomicrobium pneumaticum, and meniscus glaucopis). populations of cells were slowly (0.5 to 2.9 h) saturated with argon, nitrogen, or helium to final pressures up to 100 atm (10, 132 kpa). the gas phases of the vesicles remained intact and, upon rapid (1 to 2 s) decompression to atmospheric pressure, expanded and ruptured the cells ...19807204336
a comparison of organochlorine removal from bleached kraft pulp and paper-mill effluents by dehalogenating pseudomonas, ancylobacter and methylobacterium strains.the relative importance of each of three dechlorinating species to overall organochlorine removal from bleached kraft-mill effluents (bkme) was assessed. ancylobacter aquaticus a7, pseudomonas p1, and methylobacterium cp13, strains indigenous to a bkme treatment system, were tested for growth on chlorinated acetic acids and alcohols, and for adsorbable organic halogen (aox) reduction in batch cultures of sterile bkme from three sources. a. aquaticus a7 exhibited the broadest substrate range, but ...19957765918
expression of mosquitocidal toxin genes in a gas-vacuolated strain of ancylobacter aquaticus.a series of plasmids bearing the binary toxin genes of bacillus sphaericus 2297 or 2317.3, the 100-kda toxin gene of b. sphaericus ssii-1, or the 130-kda (cryivb) toxin gene of bacillus thuringiensis subsp. israelensis were constructed and introduced into ancylobacter aquaticus by electroporation. the transformed a. aquaticus cells exhibited significant toxicity towards mosquito larvae, demonstrating a potential use of recombinant a. aquaticus for biological control of mosquitoes.19947993101
influence of organic nutrients and cocultures on the competitive behavior of 1,2-dichloroethane-degrading bacteria.the effects of organic nutrients and cocultures on substrate removal by and competitive behavior of 1,2-dichloroethane-degrading bacteria were investigated. xanthobacter autotrophicus gj10 needed biotin for optimal growth on 1,2-dichloroethane. in continuous culture, dilution of biotin to a concentration below 0.2 nm resulted in washout. growth could be restored by inoculation with the 2-chloroethanol utilizer pseudomonas sp. strain gj1, leading to a new steady state in which about 1% of the mix ...19938250561
characterization of bacteria isolated from a bleached kraft pulp mill wastewater treatment system.water samples from the wastewater treatment system of a bleach kraft mill and from the river that supplies the mill were plated on six different media and culturable isolates were screened for substrate utilization patterns, taxonomic characters, plasmid content, and resistance to ampicillin, streptomycin, kanamycin, tetracycline, naldixic acid, mercury, nickel, copper, cobalt, cadmium, and zinc. a cluster analysis of the substrate utilization profiles and taxonomic characters revealed that pseu ...19938439869
genetic adaptation of bacteria to halogenated aliphatic compounds.the bacterial degradation and detoxification of chlorinated xenobiotic compounds requires the production of enzymes that are capable of recognizing and converting compounds which do not occur at significant concentrations in nature. we have studied the catabolic route of 1,2-dichloroethane as an example of a pathway for the conversion of such a synthetic compound. in strains of xanthobacter and ancylobacter that have been isolated on 1,2-dichloroethane, the first catabolic step is catalyzed by a ...19958565904
sulfur oxidation in rice field soil: activity, enumeration, isolation and characterization of thiosulfate-oxidizing bacteria.in rice paddy fields the bulk soil is anoxic, but oxygenated zones occur in the surrounding of the rice roots to where oxygen is transported via the aerenchyma system of the rice plants. in the anaerobic soil compartments sulfate is consumed by sulfate-reducing bacteria. in the rhizosphere the reduced sulfur compounds can be reoxidized by sulfur-oxidizing bacteria. measurements of the potential activity of thiosulfate-oxidizing bacteria in soil slurries derived from planted rice soil microcosms ...19989924825
proposal for the reclassification of thiobacillus novellus as starkeya novella gen. nov., comb. nov., in the alpha-subclass of the proteobacteria.thiobacillus novellus is a facultatively chemolithoautotrophic and methylotrophic, gram-negative, rod-shaped sulfur bacterium, shown by 16s rrna gene sequence analysis to be a member of the alpha-2 subclass of the proteobacteria. as such, it must be excluded from the genus thiobacillus, whose species are members of the beta-proteobacteria. it closest phylogenetic neighbour appears to be ancylobacter, from which it is distinct morphologically and in some physiological characteristics. it is disti ...200011034489
albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane.a novel genus, albibacter, with one species, albibacter methylovorans sp. nov., is proposed for a facultatively chemolithotrophic and methylotrophic bacterium (strain dm10t) with the ribulose bisphosphate (rubp) pathway of c1 assimilation. the bacterium is a gram-negative, aerobic, asporogenous, nonmotile, colourless rod that multiplies by binary fission. the organism utilizes dichloromethane, methanol, methylamine, formate and co2/h2, as well as a variety of polycarbon compounds, as carbon and ...200111411673
[new aerobic methyltrophic isolates from the soda lakes of the southern transbaikal].twenty-one bacterial associations isolated from the soda lakes of the southern transbaikal region were found to be able to actively grow at ph 9-10 on methanol as the source of carbon and energy. two alkalitolerant facultatively methylotrophic strains, bur 3 and bur 5, were obtained in pure cultures. both strains represent gram-negative, nonmotile, bean-shaped, encapsulated cells that reproduce by binary fission. the strains are able to grow at temperatures ranging from 6 to 42 degrees c, with a ...200111450464
phylogenetic placement of the spirosomaceae.comparative analysis of 16s rrna sequences shows that the family spirosomaceae belongs within the eubacterial phylum defined by the flavobacteria and bacteriodes. its constituent genera, spirosoma, flectobacillus, and runella form a monophyletic grouping therein. the phylogenetic assignment is based not only upon evolutionary distance analysis, but also upon sequence signatures and higher order structural synapomorphies in 16s rrna. another genus peripherally associated with the spirosomaceae ...199011538307
purification and characterization of formate dehydrogenase from ancylobacter aquaticus strain knk607m, and cloning of the gene.ancylobacter aquaticus strain knk607m, which had high nad-dependent formate dehydrogenase (fdh) activity, was newly isolated. the enzyme, purified to homogeneity, was a dimer composed of identical subunits with a molecular mass of 44 kda. the specific activity was 9.5 u/mg, and the enzyme was optimum at ph 6.3 and 50 degrees c, most stable at ph 7.0, and stable at 50 degrees c or lower. the apparent km values for formate and nad+ were 2.4 and 0.057 mm, respectively. the enzyme was specific to fo ...200312784610
molecular mapping of genes conferring field resistance to south american leaf blight ( microcyclus ulei) in rubber tree.the south american leaf blight (salb), caused by the fungus microcyclus ulei, is the major rubber tree disease in all central and south america. a population of 192 progeny individuals derived from a cross between a resistant clone and a susceptible cultivated clone was planted in a field trial in french guiana in order to evaluate the resistance parameters under real infestation conditions. the resistance type (rt), the presence of stromata (st) and the level of attack (at) were observed 20-tim ...200314504743
[specificity of bacterial response to variation of isotopic composition of water].culture growth of bacteria with different membrane lipid composition was studied in liquid media with different isotope content of water. the concentration of deuterium in water was varied from 0.01 to 90%. it was shown that large concentrations of deuterium cause the inhibition of all cultures except deinococcus radiodurans, the most stable living cell. two cultures, methylobacterium organophilum and hyphomonas jannaschiana, showed a pronounced activation at a deuterium concentration of 0.01%. ...200314515487
ancylobacter rudongensis sp. nov., isolated from roots of spartina anglica.a curved, ring-like bacterium, strain as 1.1761(t), isolated from the roots of spartina anglica, was studied by a polyphasic approach. according to phylogenetic analysis, strain as 1.1761(t) belongs to the genus ancylobacter, with 99.21 % 16s rdna sequence similarity to ancylobacter aquaticus, the only species described so far in this genus. however, strain as 1.1761(t) had no significant dna-dna binding with the type strain of a. aquaticus. in addition, strain as 1.1761(t) differed from a. aqua ...200415023948
pathway dependent isotopic fractionation during aerobic biodegradation of 1,2-dichloroethane.1,2-dichloroethane (1,2-oca) is a widespread groundwater contaminant known to be biodegradable under aerobic conditions via enzymatic oxidation or hydrolytic dehalogenation reactions. current literature reports that stable carbon isotope fractionation of 1,2-dca during aerobic biodegradation is large and reproducible (-27 to -33/1000). in this study, a significant variation in the magnitude of stable carbon isotope fractionation during aerobic biodegradation was observed. biodegradation in exper ...200415487787
[influence of the incubation temperature on the reaction of oligotrophic bacteria to stress].representatives of five genera of psychroactive oligotrophic bacteria, arcocella, renobacter, spirosoma, caulobacter, and methylobacterium, were for the first time shown to be capable of growing at a negative temperature (-2 degrees c). long-term cultivation (for 116 days) at a low temperature under limitation by the carbon source is stressful for oligotrophic bacteria and leads to the death of a part of the cell population. the number of viable cells of caulobacter crescentus decreased by two-t ...200415688934
the hierarchical system of the 'alphaproteobacteria': description of hyphomonadaceae fam. nov., xanthobacteraceae fam. nov. and erythrobacteraceae fam. nov.phylogenetic analysis of the class 'alphaproteobacteria', including physiologically diverse species, was conducted by using small-subunit rrna gene sequences. the 16s rrna gene sequences of 261 species in the class 'alphaproteobacteria' were obtained from genbank/embl/ddbj for constructing a phylogenetic tree by using maximum-likelihood analysis. in the resulting tree, members of the class 'alphaproteobacteria' were subdivided into five major clusters, which were compared with the taxonomic outl ...200516166687
predominant bacteria in an activated sludge reactor for the degradation of cutting fluids.for the first time, an activated sludge reactor, established for the degradation of cutting fluids, was examined for predominant bacteria. in addition, both total and viable numbers of bacteria in the reactor were determined so that the percentage of each predominant type in the total reactor population could be determined. three samples were studied, and a total of 15 genera were detected. in each sample, the genus pseudomonas and the genus microcyclus were present in high numbers. three other ...198316346426
aerobic microbial degradation of glucoisosaccharinic acid.alpha-glucoisosaccharinic acid (gisa), a major by-product of kraft paper manufacture, was synthesized from lactose and used as the carbon source for microbial media. ten strains of aerobic bacteria capable of growth on gisa were isolated from kraft pulp mill environments. the highest growth yields were obtained with ancylobacter spp. at ph 7.2 to 9.5. gisa was completely degraded by cultures of an ancylobacter isolate. ancylobacter cell suspensions consumed oxygen and produced carbon dioxide in ...198416346467
autotrophic growth of gas vacuolate strains of microcyclus aquaticus on methanol and hydrogen.seven strains of microcyclus aquaticus were found to be capable of growth on methanol and hydrogen as energy sources. carbon was incorporated as co(2) via the calvin cycle, as shown by the presence of ribulosebisphosphate carboxylase activity in methanol-grown cells and by the absence of key enzymes of the ribulose monophosphate and serine pathways. in addition, incoporation of [c]methanol into cells was diminished when cultures were incubated in gas atmospheres enriched with carbon dioxide.198416346525
kinetics of bacterial growth on chlorinated aliphatic compounds.with the pure bacterial cultures ancylobacter aquaticus ad20 and ad25, xanthobacter autotrophicus gj10, and pseudomonas sp. strain ad1, monod kinetics was observed during growth in chemostat cultures on 1,2-dichloroethane (ad20, ad25, and gj10), 2-chloroethanol (ad20 and gj10), and 1,3-dichloro-2-propanol (ad1). both the michaelis-menten constants (k(m)) of the first catabolic (dehalogenating) enzyme and the monod half-saturation constants (k(s)) followed the order 2-chloroethanol, 1,3-dichloro- ...199316348981
degradation of 2-chloroethylvinylether by ancylobacter aquaticus ad25 and ad27.incubation of five different beta-chloroethers with slurries prepared from brackish water sediment or activated sludge revealed that bis(2-chloroethyl)ether and 2-chloroethylvinylether (2-cve) were biodegradable under aerobic conditions. after enrichment, two different cultures of ancylobacter aquaticus that are capable of growth on 2-cve were isolated. both cultures were also able to grow on 1,2-dichloroethane. the cells contained a haloalkane dehalogenase that dehalogenated 2-cve, 2-chloroethy ...199316349032
the effects of cyanobacterial exudates on bacterial growth and biodegradation of organic contaminants.the pulp and paper industry largely depends on the biodegradation activities of heterotrophic bacteria to remove organic contaminants in wastewater prior to discharge. our recent discovery of extensive cyanobacterial communities in pulp and paper waste treatment systems led us to investigate the potential impacts of cyanobacterial exudates on growth and biodegradation efficiency of three bacterial heterotrophs. each of the three assessed bacteria represented different taxa commonly found in pulp ...200616382284
ancylobacter polymorphus sp. nov. and ancylobacter vacuolatus sp. nov.the taxonomic positions of 'renobacter vacuolatum' dsm 1277 and 'ancylobacter polymorphus' dsm 2457 were investigated in this study. 16s rrna gene sequence analysis indicated that both strains belonged to the genus ancylobacter. dna-dna hybridization showed that they differed from ancylobacter aquaticus dsm 101(t) and ancylobacter rudongensis as 1.1761(t). according to molecular and phenotypic characteristics, strain dsm 1277(t) (=as 1.2807(t)) is proposed as the type strain of ancylobacter vacu ...200616738088
[transgenic bioinsecticides inimical to parasites, but imical to environment].identification of bacillus thuringiensis (bt) parasporal crystalline inclusions composed of cry proteins (=delta-endotoxins) resulted in introduction of microbial pesticides for biological control of some parasites. delta-endotoxins are encoded by cry genes and are active against pest and nuisance insects (mostly mosquitoes and black flies--vectors of still important infectious diseases). the recent significant progress in dna recombination technique may overcome limitations (a short residual pe ...200316889013
starkeya koreensis sp. nov., isolated from rice straw.a facultatively chemolithoautotrophic, gram-negative, aerobic, highly curved rod-shaped, non-motile, non-spore-forming bacterium, strain jip08(t), was isolated from rice straw in daejeon, south korea. strain jip08(t) produced colourless colonies (1.0-1.5 mm) on r2a medium after 3 days. on the basis of 16s rrna gene sequence similarity, strain jip08(t) was shown to belong to the alpha-2 subclass of the proteobacteria, and was most closely related to starkeya novella iam 12100(t) (98.6 %), angulom ...200617012571
bypassing of a polygenic microcyclus ulei resistance in rubber tree, analyzed by qtl detection.genetic resistance components of the hevea brasiliensis x h. benthamiana ro 38 cultivar to microcyclus ulei disease were investigated by inoculating isolates which succeeded in partially or completely infecting genotypes of a mapping population. progeny of a cross between ro 38 and a susceptible cultivar was inoculated under controlled conditions with three isolates and scored for two resistance traits. interval mapping and a nonparametric test were used to detect resistance quantitative trait l ...200717204080
south american leaf blight of the rubber tree (hevea spp.): new steps in plant domestication using physiological features and molecular markers.rubber trees (hevea spp.) are perennial crops of amazonian origin that have been spread over the whole tropical belt to guarantee worldwide production of natural rubber. this crop plant has found its place in many national economies of producing countries, and although its domestication by selection of suitable genotypes was very slow, it contributes a lot to the welfare of small farmers worldwide. its development is limited by severe diseases. in south america, the main fungal disease of rubber ...200717650512
guayule and russian dandelion as alternative sources of natural rubber.natural rubber, obtained almost exclusively from the para rubber tree (hevea brasiliensis), is a unique biopolymer of strategic importance that, in many of its most significant applications, cannot be replaced by synthetic rubber alternatives. several pressing motives lead to the search for alternative sources of natural rubber. these include increased evidence of allergenic reactions to hevea rubber, the danger that the fungal pathogen microcyclus ulei, causative agent of south american leaf bl ...200718085463
novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments.arsenic oxidation is recognized as being mediated by both heterotrophic and chemoautotrophic microorganisms. enrichment cultures were established to determine whether chemoautotrophic microorganisms capable of oxidizing arsenite as(iii) to arsenate as(v) are present in selected contaminated but nonextreme environments. three new organisms, designated as strains ol-1, s-1 and cl-3, were isolated and found to oxidize 10 mm arsenite to arsenate under aerobic conditions using co2-bicarbonate (co2/hc ...200818717738
description of ancylobacter oerskovii sp. nov. and two additional strains of ancylobacter polymorphus.a gram-negative, pleomorphic, rod-shaped, non-spore-forming bacterium, designated strain ns05t, was isolated from soil after enrichment with oxalate. on the basis of 16s rrna gene sequence similarity, strain ns05t was shown to be phylogenetically related to the genera ancylobacter, starkeya and angulomicrobium (96.3-98.1% sequence similarity), class alphaproteobacteria. strain ns05t was most closely related to ancylobacter rudongensis as 1.1761t (98.1% sequence similarity). the whole-cell fatty ...200818768594
ancylobacter dichloromethanicus sp. nov.--a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane.a novel aerobic facultative methylotroph was isolated from contaminated soil. the organism (strain dm16) is a gram-negative asporogenous non-motile curved rod multiplying by binary fission. cells are neutrophilic and mesophilic. this strain utilized dichloromethane, methanol, formate and formaldehyde along with a variety of polycarbon compounds. strain dm16 employs the ribulosebisphosphate pathway for c1 assimilation. the dna g+c content is 64.5 mol%. the major ubiquinone is q-10. the dominant c ...200919346095
characterization of an isoproturon mineralizing bacterial culture enriched from a french agricultural soil.the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (ipu), was found to be rapidly mineralized by a bacterial culture isolated from an agricultural soil regularly exposed to ipu. molecular analysis of the bacterial culture by dna fingerprinting, cloning and sequencing of the 16s rrna genes revealed that it consisted of six different members among whom the dominant was related to sphingomonas sp. six bacterial strains belonging to genera ancylobacter, pseudomonas, stenotr ...200919836052
arsenic-resistant bacteria associated with roots of the wild cirsium arvense (l.) plant from an arsenic polluted soil, and screening of potential plant growth-promoting characteristics.a rhizobacterial community, associated with the roots of wild thistle cirsium arvense (l.) growing in an arsenic polluted soil, was studied by fluorescence in situ hybridization (fish) analysis in conjunction with cultivation-based methods. in the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative picture obtained by fish analysis of the main phylogenetic bacterial groups was similar and was predominantly comprised of alphaproteobacteria, betaproteobacteria, and gammaproteo ...201020303688
prevalence of the gene trzn and biogeographic patterns among atrazine-degrading bacteria isolated from 13 colombian agricultural soils.the following study evaluated the diversity and biogeography of 83 new atrazine-degrading bacteria and the composition of their atrazine degradation genes. these strains were isolated from 13 agricultural soils and grouped according to rep-pcr genomic fingerprinting into 11 major clusters, which showed biogeographic patterns. three clusters (54 strains) belonged to the genus arthrobacter, seven clusters (28 strains) were similar to the genus nocardioides and only one strain was a gram-negative f ...201020597985
study of biochemical pathway and enzyme involved in metsulfuron-methyl degradation by ancylobacter sp. xj-412-1 isolated from soil.ancylobacter sp. xj-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. when metsulfuron-methyl was provided as the sole carbon source, more than 90.5% of metsulfuron-methyl at concentration of 50 mg l(-1) was degraded by strain xj-412-1 after incubation at 30°c for 7 days. the initial degradation products of metsulfuron-methyl (msm), thifensulfuron-methyl (tsm), and bensulfuron-methyl (bsm) by xj-412-1 were identified as corresponding deesterified d ...201121431836
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