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major outer membrane proteins unique to reproductive cells of hyphomonas jannaschiana.separation on the basis of molecular weight resolved three proteins specific to the swarmer cell of hyphomonas jannaschiana. in the reproductive cell, 4 major proteins were identified as cytoplasmic and 10 were identified as envelope. of these envelope proteins, one was common to both the inner and outer membranes, four were common to the inner membrane, and five were common to the outer membrane. four of these outer membrane proteins were specific to the reproductive cell, and two of these prot ...19892703471
isolation and characterization of novel psychrophilic, neutrophilic, fe-oxidizing, chemolithoautotrophic alpha- and gamma-proteobacteria from the deep sea.we report the isolation and physiological characterization of novel, psychrophilic, iron-oxidizing bacteria (feob) from low-temperature weathering habitats in the vicinity of the juan de fuca deep-sea hydrothermal area. the feob were cultured from the surfaces of weathered rock and metalliferous sediments. they are capable of growth on a variety of natural and synthetic solid rock and mineral substrates, such as pyrite (fes(2)), basalt glass ( approximately 10 wt% feo), and siderite (feco(3)), a ...200312732565
spatial and temporal deposition of adhesive extracellular polysaccharide capsule and fimbriae by hyphomonas strain mhs-3.hyphomonas strain mhs-3, a member of a genus of primary colonizers of surfaces immersed in marine water, synthesizes two structures that mediate adhesion to solid substrata, namely, capsular exopolysaccharide and fimbriae. specific stains, gold-labelled lectins, and monoclonal antibodies, along with transmission electron microscopy of synchronized populations, revealed that both structures are polarly and temporally expressed. the timed synthesis and placement of the fimbriae and capsule correla ...199816349537
a novel lineage of proteobacteria involved in formation of marine fe-oxidizing microbial mat communities.for decades it has been recognized that neutrophilic fe-oxidizing bacteria (feob) are associated with hydrothermal venting of fe(ii)-rich fluids associated with seamounts in the world's oceans. the evidence was based almost entirely on the mineralogical remains of the microbes, which themselves had neither been brought into culture or been assigned to a specific phylogenetic clade. we have used both cultivation and cultivation-independent techniques to study fe-rich microbial mats associated wit ...200717668050
plasmids of psychrophilic and psychrotolerant bacteria and their role in adaptation to cold environments.extremely cold environments are a challenge for all organisms. they are mostly inhabited by psychrophilic and psychrotolerant bacteria, which employ various strategies to cope with the cold. such harsh environments are often highly vulnerable to the influence of external factors and may undergo frequent dynamic changes. the rapid adjustment of bacteria to changing environmental conditions is crucial for their survival. such "short-term" evolution is often enabled by plasmids-extrachromosomal rep ...201425426110
the s-layer glycome-adding to the sugar coat of bacteria.the amazing repertoire of glycoconjugates present on bacterial cell surfaces includes lipopolysaccharides, capsular polysaccharides, lipooligosaccharides, exopolysaccharides, and glycoproteins. while the former are constituents of gram-negative cells, we review here the cell surface s-layer glycoproteins of gram-positive bacteria. s-layer glycoproteins have the unique feature of self-assembling into 2d lattices providing a display matrix for glycans with periodicity at the nanometer scale. typic ...201020871840
the s-layer glycome-adding to the sugar coat of bacteria.the amazing repertoire of glycoconjugates present on bacterial cell surfaces includes lipopolysaccharides, capsular polysaccharides, lipooligosaccharides, exopolysaccharides, and glycoproteins. while the former are constituents of gram-negative cells, we review here the cell surface s-layer glycoproteins of gram-positive bacteria. s-layer glycoproteins have the unique feature of self-assembling into 2d lattices providing a display matrix for glycans with periodicity at the nanometer scale. typic ...201020871840
the presence of nitrate dramatically changed the predominant microbial community in perchlorate degrading cultures under saline conditions.perchlorate contamination has been detected in both ground water and drinking water. an attractive treatment option is the use of ion-exchange to remove and concentrate perchlorate in brine. biological treatment can subsequently remove the perchlorate from the brine. when nitrate is present, it will also be concentrated in the brine and must also be removed by biological treatment. the primary objective was to obtain an in-depth characterization of the microbial populations of two salt-tolerant ...201425194715
complete genome sequence of hirschia baltica type strain (ifam 1418(t)).the family hyphomonadaceae within the alphaproteobacteria is largely comprised of bacteria isolated from marine environments with striking morphologies and an unusual mode of cell growth. here, we report the complete genome sequence hirschia baltica, which is only the second a member of the hyphomonadaceae with a published genome sequence. h. baltica is of special interest because it has a dimorphic life cycle and is a stalked, budding bacterium. the 3,455,622 bp long chromosome and 84,492 bp pl ...201122675580
lipidblast templates as flexible tools for creating new in-silico tandem mass spectral libraries.tandem mass spectral libraries (ms/ms) are usually built by acquiring experimentally measured mass spectra from chemical reference compounds. we here show the versatility of in-silico or computer generated tandem mass spectra that are directly obtained from compound structures. we use the freely available lipidblast development software to generate 15 000 ms/ms spectra of the glucuronosyldiacylglycerol (glcadg) lipid class, recently discovered for the first time in plants. the generation of such ...201425340521
bioprocessing data for the production of marine enzymes.this review is a synopsis of different bioprocess engineering approaches adopted for the production of marine enzymes. three major modes of operation: batch, fed-batch and continuous have been used for production of enzymes (such as protease, chitinase, agarase, peroxidase) mainly from marine bacteria and fungi on a laboratory bioreactor and pilot plant scales. submerged, immobilized and solid-state processes in batch mode were widely employed. the fed-batch process was also applied in several b ...201020479981
multilocus sequence analysis for the assessment of phylogenetic diversity and biogeography in hyphomonas bacteria from diverse marine environments.hyphomonas, a genus of budding, prosthecate bacteria, are primarily found in the marine environment. seven type strains, and 35 strains from our collections of hyphomonas, isolated from the pacific ocean, atlantic ocean, arctic ocean, south china sea and the baltic sea, were investigated in this study using multilocus sequence analysis (mlsa). the phylogenetic structure of these bacteria was evaluated using the 16s rrna gene, and five housekeeping genes (leua, clpa, pyrh, gata and rpod) as well ...201425019154
thalassospira sp. isolated from the oligotrophic eastern mediterranean sea exhibits chemotaxis toward inorganic phosphate during starvation.the eastern mediterranean sea represents an ultraoligotrophic environment where soluble phosphate limits the growth of bacterioplankton. correspondingly, genes coding for high affinity phosphate uptake systems and for organophosphonate utilization are highly prevalent in the plankton metagenome. chemotaxis toward inorganic phosphate constitutes an alternative strategy to cope with phosphate limitation, but so far has only been demonstrated for two bacterial pathogens and an archaeon, and not in ...201121602377
bacterial community structure associated with a dimethylsulfoniopropionate-producing north atlantic algal bloom.the bacteria associated with oceanic algal blooms are acknowledged to play important roles in carbon, nitrogen, and sulfur cycling, yet little information is available on their identities or phylogenetic affiliations. three culture-independent methods were used to characterize bacteria from a dimethylsulfoniopropionate (dmsp)-producing algal bloom in the north atlantic. group-specific 16s rrna-targeted oligonucleotides, 16s ribosomal dna (rdna) clone libraries, and terminal restriction fragment ...200011010865
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