numerical taxonomy and ecology of oligotrophic bacteria isolated from the estuarine environment. | slow-growing bacteria, isolated on nutrient-rich and nutrient-limited media, from chesapeake bay water and sediment samples, were examined for 119 biochemical, cultural, morphological, nutritional, and physiological characters. those bacteria which grow on low nutrient media, termed oligotrophs, a total of 162 strains, were subjected to taxonomic analysis, as a preliminary step in determining their ecological significance. the data for all strains included in the study were examined by computer ... | 1977 | 871972 |
expression of cloned restriction and modification genes, hjairm from hyphomonas jannaschiana in escherichia coli. | a type-ii rm system, hjai, was identified in the marine bacterium, hyphomonas jannaschiana. the enase recognizes gatatc, and dna fragments generated after cleavage with this enzyme contain blunt ends. a dna fragment encoding these enzymes was cloned and expressed in escherichia coli, although the level of expression of the cloned genes was low. dna methylated by m.hjai was not restricted by the mcr or mrr restriction systems of e. coli. although h. jannaschiana is a marine bacterium isolated nea ... | 1990 | 2197177 |
a phylogenetic survey of budding, and/or prosthecate, non-phototrophic eubacteria: membership of hyphomicrobium, hyphomonas, pedomicrobium, filomicrobium, caulobacter and "dichotomicrobium" to the alpha-subdivision of purple non-sulfur bacteria. | the phylogenetic position of various budding and/or or prosthecate gram-negative eubacteria was determined by different methods. members of the genera hyphomicrobium, filomicrobium, pedomicrobium were investigated by 16s rrna cataloguing, a 1373 nucleotide long portion of the 16s rrna was sequenced from hyphomicrobium vulgare and the 5s rrnas were analyzed from two hyphomicrobium strains, hyphomonas polymorpha and caulobacter crescentus. comparison with published sequences indicated a membership ... | 1988 | 2455491 |
deoxyribonucleic acid base sequence homologies of some budding and prosthecate bacteria. | the genetic relatedness of a number of budding and prosthecate bacteria was determined by deoxyribonucleic acid (dna) homology experiments of the direct binding type. strains of hyphomicrobium sp. isolated from aquatic habitats were found to have relatedness values ranging from 9 to 70% with strain "ea-617," a subculture of the hyphomicrobium isolated by mevius from river water. strains obtained from soil enrichments had lower values with ea-617, ranging from 3 to 5%. very little or no homology ... | 1972 | 5018022 |
diversity of uncultured microorganisms associated with the seagrass halophila stipulacea estimated by restriction fragment length polymorphism analysis of pcr-amplified 16s rrna genes. | the diversity of microorganisms associated with the leaves of the seagrass halophila stipulacea in the northern gulf of elat was examined by culture-independent analysis. microorganisms were harvested by a sonication treatment for total-community genomic dna isolation. oligonucleotides complementary to conserved regions in the 16s rrna-encoding dna (rdna) of bacteria were used for pcr amplification. the 16s rdna pcr products were subcloned and further characterized by a restriction fragment leng ... | 1996 | 8975607 |
diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
[element composition of bacterial cells of various taxa]. | the elemental compositions of cells of representatives of five bacterial genera (hyphomonas vulgare np-160, caulobacter bacteroides np-105, flectobacillus major mm, escherichia coli, and spirulina platensis) was studied by electron microscopic x-ray microanalysis. the contents of p, s, cl, k, and ca were determined. the bacterial species studied in this work substantially differ by the contents of these elements. a high content of k is typical of s. platensis, whereas f. major, an aquatic form, ... | 1998 | 9567294 |
isolation and characterization of s-layer proteins from a vent prosthecate bacterium. | mwapp 116,000 and 29,000 proteins (p116 and p29), major outer membrane proteins of hyphomonas jannaschiana reproductive cells, were extracted from cell envelopes by dialysis against edta, 2 m urea or distilled water. these proteins were precipitated by divalent cations and resolubilized by edta-na, reflecting alternate monomer, multimer states. from two-dimensional gel electrophoresis it was determined that p116 and p29 had a pl of 4.5. both were glycoproteins. results suggest that p116 and p29 ... | 1998 | 9670553 |
isolation and characterization of novel psychrophilic, neutrophilic, fe-oxidizing, chemolithoautotrophic alpha- and gamma-proteobacteria from the deep sea. | we report the isolation and physiological characterization of novel, psychrophilic, iron-oxidizing bacteria (feob) from low-temperature weathering habitats in the vicinity of the juan de fuca deep-sea hydrothermal area. the feob were cultured from the surfaces of weathered rock and metalliferous sediments. they are capable of growth on a variety of natural and synthetic solid rock and mineral substrates, such as pyrite (fes(2)), basalt glass ( approximately 10 wt% feo), and siderite (feco(3)), a ... | 2003 | 12732565 |
predominant bacteria in an activated sludge reactor for the degradation of cutting fluids. | for the first time, an activated sludge reactor, established for the degradation of cutting fluids, was examined for predominant bacteria. in addition, both total and viable numbers of bacteria in the reactor were determined so that the percentage of each predominant type in the total reactor population could be determined. three samples were studied, and a total of 15 genera were detected. in each sample, the genus pseudomonas and the genus microcyclus were present in high numbers. three other ... | 1983 | 16346426 |
interactions between shewanella colwelliana, oyster larvae, and hydrophobic organophosphate pesticides. | shewanella colwelliana (strain d) is a periphytic estuarine bacterium that forms biofilms beneficial to oyster set. our study examined whether these and other films concentrated two hydrophobic, organophosphate pesticides, abate and malathion, that are detected in chesapeake bay oyster waters. both biofilms and purified exopolysaccharide of s. colwelliana did not adsorb more of the abate or malathion than could be accounted for by adsorption to control surfaces. similar results were obtained by ... | 1990 | 16348382 |
spatial and temporal deposition of adhesive extracellular polysaccharide capsule and fimbriae by hyphomonas strain mhs-3. | hyphomonas strain mhs-3, a member of a genus of primary colonizers of surfaces immersed in marine water, synthesizes two structures that mediate adhesion to solid substrata, namely, capsular exopolysaccharide and fimbriae. specific stains, gold-labelled lectins, and monoclonal antibodies, along with transmission electron microscopy of synchronized populations, revealed that both structures are polarly and temporally expressed. the timed synthesis and placement of the fimbriae and capsule correla ... | 1998 | 16349537 |
high numbers of prosthecate bacteria in pulp mill waste aeration lagoons. | prosthecate bacteria comprised 0.6 to 10.5% of the bacterial community in samples from 11 pulp mill waste aeration lagoons. because of their distinct morphology, the genera ancalomicrobium, caulobacter, prosthecobacter, prosthecomicrobium, stella, and hyphomicrobium or hyphomonas could be identified and enumerated by direct microscopic examination. monthly samples from one lagoon showed that several genera varied from undetectable to predominant among the appendaged organisms. temperature (seaso ... | 1979 | 16345371 |
the hierarchical system of the 'alphaproteobacteria': description of hyphomonadaceae fam. nov., xanthobacteraceae fam. nov. and erythrobacteraceae fam. nov. | phylogenetic analysis of the class 'alphaproteobacteria', including physiologically diverse species, was conducted by using small-subunit rrna gene sequences. the 16s rrna gene sequences of 261 species in the class 'alphaproteobacteria' were obtained from genbank/embl/ddbj for constructing a phylogenetic tree by using maximum-likelihood analysis. in the resulting tree, members of the class 'alphaproteobacteria' were subdivided into five major clusters, which were compared with the taxonomic outl ... | 2005 | 16166687 |
mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate alexandrium tamarense (lebour) balech. | five bacterial strains were isolated from a non-toxigenic strain of the marine dinoflagellate alexandrium tamarense (lebour) balech ccmp 116 (nepcc c116), during a survey of the diversity of bacteria associated with paralytic shellfish toxin-producing cultures of alexandrium spp. (dinophyta). all strains were strictly aerobic, gram-negative, straight or curved rods. cells were dimorphic, with stalks (or prosthecae) and non-motile or non-stalked and motile, by means of a single polar flagellum. t ... | 2003 | 14657121 |
[specificity of bacterial response to variation of isotopic composition of water]. | culture growth of bacteria with different membrane lipid composition was studied in liquid media with different isotope content of water. the concentration of deuterium in water was varied from 0.01 to 90%. it was shown that large concentrations of deuterium cause the inhibition of all cultures except deinococcus radiodurans, the most stable living cell. two cultures, methylobacterium organophilum and hyphomonas jannaschiana, showed a pronounced activation at a deuterium concentration of 0.01%. ... | 2003 | 14515487 |
the polar polysaccharide capsule of hyphomonas adhaerens mhs-3 has a strong affinity for gold. | select groups of bacteria, including prothescate species, have an unusual capacity to sequester gold and bioconcentrate it to very high levels. hyphomonas adhaerens mhs-3 (mhs-3) is one such species, as demonstrated by energy dispersive spectroscopy. transmission electron microscopy revealed that the binding site was specific on the polar polysaccharide capsule. a capsuleless mutant and periodate-treated wild type did not sequester gold. the gold may interact with the same sites in the capsule t ... | 2001 | 11598803 |
phylogenetic analysis of bacterial communities associated with leaves of the seagrass halophila stipulacea by a culture-independent small-subunit rrna gene approach. | the phylogenetic diversity of the bacterial community associated with leaves of the marine plant halophila stipulacea in the northern gulf of elat was examined by 16s rrna gene (rdna) sequence analyses of a clone library. for 59 clones corresponding to 51 ardra (amplified rdna restriction analysis) groups, the sequence of approximately 1 kb was determined, and the fraction of the corresponding ardra groups of the leaf library was calculated. the class proteobacteria was represented by 62.6% of t ... | 2000 | 10790514 |
hyphomonas adhaerens sp. nov., hyphomonas johnsonii sp. nov. and hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. | three strains of prosthecate, budding bacteria, mhs-2t, mhs-3t and vp6t, were isolated from marine habitats including the open ocean (the pelagic zone), the offshore region (the neritic zone) and the hydrothermal vent region. a polyphasic approach including 16s rdna sequencing, phenotypic analyses, serology, fatty acid analyses, membrane protein profiles and dna-dna hybridizations was used to place these strains in the genus hyphomonas, a taxon of the alpha-proteobacteria. the results of these a ... | 2000 | 10758848 |
[endophytic bacterial diversity in glycyrrhiza inflata bat. from xinjiang by culture-independent method]. | we investigated endophytic bacterial diversity in glycyrrhiza inflata bat. from xinjiang. | 2009 | 19673406 |
the phylogenetic relationships of caulobacter, asticcacaulis and brevundimonas species and their taxonomic implications. | the phylogenetic relationships among the species of caulobacter, asticcacaulis and brevundimonas were studied by comparison of their 16s rdna sequences. the analysis of almost complete sequences confirmed the early evolutionary divergence of the freshwater and marine species of caulobacter reported previously [stahl, d. a., key, r., flesher, b. & smit, j. (1992). j bacteriol 174, 2193-2198]. the freshwater species formed two distinct clusters. one cluster contained the species caulobacter bacter ... | 1999 | 10319468 |
influence of divalent cations and ph on adsorption of a bacterial polysaccharide adhesin. | hyphomonas mhs-3 (mhs-3) elaborates a diffuse capsular material, primarily composed of polysaccharide, which has been implicated to serve as the holdfast of this prosthecate marine bacterium. a purified polysaccharide (fr2ps) from this capsular material exhibits a relatively large affinity for (ge), or more precisely for the ge oxide surface film. in its natural habitat mhs-3 attaches to marine sediments. this suggests that molecular properties of fr2ps have evolved to render it adhesive toward ... | 1998 | 9710502 |
fine-structure evidence for cell membrane partitioning of the nucleoid and cytoplasm during bud formation in hyphomonas species. | hyphomonas spp. reproduce by budding from the tip of the prosthecum, distal to the main body of the reproductive cell; thus, the chromosome must travel through the prosthecum to enter the progeny, the swarm cell. when viewed by electron microscopy, negatively stained whole cells, ultrathin-sectioned cells, and freeze-etched and frozen hydrated cells all had marked swellings of the cytoplasmic membrane (cm) in the prosthecum which are termed pseudovesicles (pv). pv were separated by constrictions ... | 1997 | 8981992 |
genomic analysis of hyphomonas neptunium contradicts 16s rrna gene-based phylogenetic analysis: implications for the taxonomy of the orders 'rhodobacterales' and caulobacterales. | hyphomonas neptunium is a marine prosthecate alpha-proteobacterium currently classified as a member of the order 'rhodobacterales'. although this classification is supported by 16s rrna gene sequence phylogeny, 23s rrna gene sequence analysis, concatenated ribosomal proteins, hsp70 and ef-tu phylogenies all support classifying hyphomonas neptunium as a member of the caulobacterales instead. the possible reasons why the 16s rrna gene sequence gives conflicting results in this case are also discus ... | 2005 | 15879228 |
a novel glycolipid, 1,2-diacyl-3-alpha-d-glucuronopyranosyl-sn-glycerol taurineamide, from the budding seawater bacterium hyphomonas jannaschiana. | a previously unknown glycolipid has been isolated from the budding seawater bacterium hyphomonas jannaschiana vp-2t devoid of phospholipids. using a combination of chromatographic, spectrometric, enzymic- and chemical-degradation methods, the structure of the lipid has been determined to be 1,2-diacyl-3-alpha-d-glucuronopyranosyl-sn-glycerol taurineamide. its main fatty acyls are n-16:0, cis-16:1 omega 7, n-18:0, cis-18:1 omega 7, and n-19:0. the hydroxyl at the sn-1 position of the glycerol res ... | 1996 | 8695667 |
homogentisic acid is the primary precursor of melanin synthesis in vibrio cholerae, a hyphomonas strain, and shewanella colwelliana. | the enzyme p-hydroxyphenylpyruvate hydroxylase (hpph) is involved in pigmentation (pyomelanin) via homogentisic acid (hga). pyomelanin formation is correlated with hga production and expression of hpph in three disparate marine species: vibrio cholerae, a hyphomonas strain, and shewanella colwelliana. induction of pigmentation in v. cholerae 569b by nutrient limitation also correlated with production of hga. | 1995 | 7747976 |
serological relationships among budding, prosthecate bacteria. | the somatic antigens of 25 strains of budding bacteria were typed and 14 serologically distinct groups were identified, suggesting considerable antigenic diversity among hyphomicrobia. ten of the groups were represented by a single isolate, 2 contained two isolates, 1 three isolates, and 1 eight isolates. the strains in the largest group of eight isolates each shared at least one common antigen. however, there was also considerable antigenic heterogeneity within this cluster. serological activit ... | 1980 | 6157462 |
new bacteriophages active on strains of hyphomicrobium. | fifty-five lytic bacteriophages isolated from water and soil samples were active on many strains of the genus hyphomicrobium. the optimal isolation procedure was an adsorption method in which samples from a habitat similar to that of the respective host bacterium were used as the phage inoculum. according to the morphology and nucleic acid type these bacteriophages belonged to different families: myoviridae (type a1: five phages); styloviridae (type b1: 33 phages; type b2: eight phages) and podo ... | 1988 | 3199100 |
taxonomic and phylogenetic studies on a new taxon of budding, hyphal proteobacteria, hirschia baltica gen. nov., sp. nov. | four strains of budding, hyphal bacteria, which had very similar chemotaxonomic properties, were isolated from the baltic sea. the results of dna-dna hybridization experiments, indicated that three of the new isolates were closely related, while the fourth was only moderately related to the other three. sequence signature and higher-order structural detail analyses of the 16s rrna of strain ifam 1418t (t = type strain) indicated that this isolate is related to the alpha subclass of the class pro ... | 1990 | 2275859 |
evolutionary relationship of some stalked and budding bacteria (genera caulobacter, "hyphobacter", hyphomonas and hyphomicrobium) as studied by the new integral taxonomical method. | a new approach was developed for the determination of taxonomic and evolutional relationships among four genera of oligotrophic bacteria. the main idea of this approach is the algorithmized integrative analysis of the morphological and physiological specificity of these bacteria, their 5s rrna sequences, fatty acid and lipid composition of their membranes, as well as their sensitivity to a large variety of antibiotics. it was shown that the genera caulobacter and hyphomonas are closely related t ... | 1990 | 1689139 |
ribosomal ribonucleic acid cistron homologies among hyphomicrobium and various other bacteria. | the extent of hybrid formation between the ribosomal ribonucleic acid (r-rna) of hyphomicrobium strain b-522 and deoxyribonucleic acid (dna) from bacteria of 21 different genera was examined. three generalized groupings were formed. group i (72-100%) consisted entirely of other strains of hyphomicrobium. representatives of the genera rhodopseudomonas, chromatium, caulobacter, prosthecomicrobium, rhodomicrobium, hyphomonas, and hyphomicrobium made up group ii (49-69%). the remaining gram-negative ... | 1977 | 861853 |
major outer membrane proteins unique to reproductive cells of hyphomonas jannaschiana. | separation on the basis of molecular weight resolved three proteins specific to the swarmer cell of hyphomonas jannaschiana. in the reproductive cell, 4 major proteins were identified as cytoplasmic and 10 were identified as envelope. of these envelope proteins, one was common to both the inner and outer membranes, four were common to the inner membrane, and five were common to the outer membrane. four of these outer membrane proteins were specific to the reproductive cell, and two of these prot ... | 1989 | 2703471 |
lipid composition of the gram-negative, budding, seawater bacterium hyphomonas jannaschiana lacking in phospholipids. | the extractable polar lipids of the gram-negative, budding, seawater bacterium hyphomonas jannaschiana vp-2t were shown to consist of five glycolipids, namely, 1,2-diacyl-3-alpha-d-glucopyranosyl-sn-glycerol (i) (25% of the total lipids), 1,2-diacyl-3-alpha-d-glucuronopyranosyl-sn-glycerol (ii) (41%), taurine-amide of ii (iii) (32%), acyl-d-glucuronopyranoside (iv) (0.9%), and acyl-3-o-acyl-d-glucuronopyranoside (v) (0.4%); glycolipid (iii) was described by the authors previously. in addition, s ... | 1996 | 8816851 |
evidence for the adhesive function of the exopolysaccharide of hyphomonas strain mhs-3 in its attachment to surfaces. | hyphomonas strain mhs-3 (mhs-3) is a marine procaryote with a biphasic life cycle and which has prosthecate stages that adhere to submerged substrata. we found that adherent forms produced an exopolysaccharide (eps) capsule that bound glycine max lectin, arachis hypogaea lectin, and bauhinia purpurea lectin (bpa), each having affinity for n-acetyl-d-galactosamine. it also bound the dye calcofluor. bpa and calcofluor were tested for the ability to hinder mhs-3 adhesion to glass surfaces; they red ... | 1995 | 16535028 |
hyphomonas spp., shewanella spp., and other marine bacteria lack heterogeneous (ladderlike) lipopolysaccharides. | the lipopolysaccharides (lps) of 19 marine bacteria were examined for size heterogeneities by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis in conjunction with an lps-specific silver staining method. fifteen marine bacteria had an r-type lps instead of the ladderlike lps array characteristic of most bacteria. in addition, three marine bacteria also had a single large lps molecule. without constraints (e.g., surface masking), r-type lps, a more hydrophobic molecule, predominates ... | 1991 | 16348529 |
sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in qingdao, china. | the taxonomic position of strain lmg 23452(t), which was isolated from coastal sediment from an aquaculture site near qingdao, china, in 2000, was determined. strain lmg 23452(t) comprised gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, chemoheterotroph that produces catalase and oxidase. comparative 16s rrna gene sequence analysis revealed that strain lmg 23452(t) shared approximately 89 % sequence similarity with members of the genera devosia, hyphomo ... | 2007 | 17220452 |
robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family hyphomonadaceae, from antarctic seawater. | a seawater bacterium, designated imcc3195t, was isolated from the antarctic coast. cells of the novel strain were gram-negative, rusty-coloured, strictly aerobic, chemoheterotrophic, non-budding and non-motile rods or vibrioids that possessed a thin prostheca. based on 16s rrna gene sequence comparisons, the novel strain was most closely related to the genera hyphomonas (89.4-90.9%), maricaulis (90.1-90.4%), hirschia (89.0%) and oceanicaulis (87.9%) of the family hyphomonadaceae. phylogenetic an ... | 2007 | 17978224 |
hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the mediterranean sea. | a novel aerobic, heterotrophic, prosthecate bacterium, designated 26iii/a02/215(t), was isolated from surface water of the north-western mediterranean sea. cells stained gram-negative and were straight to slightly curved rods, forming red colonies on agar plates. the strain grew at 15-37 degrees c inclusive (optimum 30 degrees c) and grew optimally at seawater salinity. growth was observed on organic acids, amino acids and complex organic substrates. the fatty acids (>5 %) detected in strain 26i ... | 2008 | 18984685 |
novel marine carbazole-degrading bacteria. | eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ... | 2009 | 19187209 |
henriciella marina gen. nov., sp. nov., a novel member of the family hyphomonadaceae isolated from the east sea. | a bacterial strain, designated iso4(t), was isolated from the east sea of korea and was subjected to a poly-phasic taxonomy study including phenotypic and chemotaxonomic characteristics as well as 16s rrna gene sequence analysis. cells of the strain were gram-negative, motile, non-budding, non-stalked, and strictly aerobic. strain iso4(t) grew optimally at 20 degrees c in the presence of 1 approximately 2% (w/v) nacl and at ph 6.9 approximately 7.6. the major respiratory quinone was q-10 and the ... | 2009 | 19412598 |
ponticaulis koreensis gen. nov., sp. nov., a new member of the family hyphomonadaceae isolated from seawater. | an obligately halophilic, stalked bacterium, designated strain gsw-23(t), was isolated from seawater that had been collected on the coast of jeju, korea. cells of the strain were characteristically gram-negative, aerobic, chemo-organotrophic, non-budding, motile rods or vibrioids that possessed prosthecae and holdfasts. multiplication occurred by means of binary fission. the major ubiquinone was q-10. the dominant cellular fatty acids were summed feature 7 (one or more of c(18 : 1)omega9c, c(18 ... | 2009 | 19628598 |
maribaculum marinum gen. nov., sp. nov., isolated from deep seawater. | a taxonomic study was carried out on strain p38(t), which was isolated from an enriched polycyclic aromatic hydrocarbon-degrading consortium from a deep seawater sample collected from the indian ocean. phylogenetic analyses based on 16s rrna gene sequences showed that strain p38(t) formed a distinct evolutionary lineage within the family hyphomonadaceae. strain p38(t) was most closely related to members of the genera hyphomonas (92.3-93.5 % 16s rrna gene sequence similarity), hirschia (88.8 %), ... | 2009 | 19643895 |
performance of a three-stage membrane bioprocess treating the organic fraction of municipal solid waste and evolution of its archaeal and bacterial ecology. | a novel three-stage bioprocess achieved 75% volatile solids (vs) removal at an organic loading rate (olr) of 4g vsl(-1)day, a solids retention time (srt) of 66days, a hydraulic retention time (hrt) of 20days, at a temperature of 35 degrees c. the bioprocess consisted of an anaerobic hydrolytic reactor (hr) where the solids and liquid fractions of the organic fraction of the municipal solid waste (ofmsw) were separated with a mesh. the leachate was pumped to a submerged anaerobic membrane bioreac ... | 2010 | 19857960 |
genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and -lactonase. | microbial virulence and their resistance to multiple drugs have obliged researchers to look for novel drug targets. virulence of pathogenic microbes is regulated by signal molecules such as acylated homoserine lactone (ahl) produced during a cell density dependent phenomenon of quorum sensing (qs). in contrast, certain microbes produce ahl-lactonases and -acylases to degrade qs signals, also termed as quorum quenching. mining sequenced genome databases has revealed organisms possessing conserved ... | 2011 | 21660112 |
influence of protein conditioning films on binding of a bacterial polysaccharide adhesin from hyphomonas mhs-3. | a putative polysaccharide adhesin which mediates non-specific attachment of hyphomonas mhs-3 (mhs-3) to hydrophilic substrata has been isolated and partially characterized. a polysaccharide-enriched portion of the extracellular polymeric substance (eps(p)) from mhs-3 was separated into four fractions using high performance size exclusion chromatography (hpsec). comparison of chromatograms of eps(p) from mhs-3 and a reduced adhesion strain (mhs-3 rad) suggested that one eps(p) fraction, which con ... | 1996 | 22115100 |
spatial and temporal deposition of hyphomonas strain vp-6 capsules involved in biofilm formation | hyphomonas strain vp-6 is a prosthecate bacterium isolated from the guayamas vent region and is a member of a genus of primary and common colonizers of marine surfaces. it adheres to solid substrata as a first step in biofilm formation. fine-structure microscopy and the use of specific stains and lectins reveal that it synthesizes two different extracellular polymeric substances (eps). one is a temporally synthesized, polar holdfast eps, and the other is a capsular eps that is present during the ... | 1998 | 9687449 |
phylogeny and polyphasic taxonomy of caulobacter species. proposal of maricaulis gen. nov. with maricaulis maris (poindexter) comb. nov. as the type species, and emended description of the genera brevundimonas and caulobacter. | the genus caulobacter is composed of prosthecate bacteria often specialized for oligotrophic environments. the taxonomy of caulobacter has relied primarily upon morphological criteria: a strain that visually appeared to be a member of the caulobacter has generally been called one without challenge. a polyphasic approach, comprising 16s rdna sequencing, profiling restriction fragments of 16s-23s rdna interspacer regions, lipid analysis, immunological profiling and salt tolerance characterizations ... | 1999 | 10425763 |
ecology and taxonomy of bacteria attaching to wood surfaces in a tropical harbor. | water, sediment, and wooden pilings, samples of which were collected from a harbor in puerto rico during the course of a long-term study of biofouling of wood treated with creosote and related compounds, were found to support growth of microbial populations, the dominant taxa of which included hyphomicrobium, hyphomonas, pseudomonas, vibrio, and bacillus. new wood exposed to the harbor water was rapidly colonized by hyphomicrobium vulgare. old pilings in an advanced stage of biodeterioration mai ... | 1979 | 487291 |
comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria hyphomonas neptunium and caulobacter crescentus. | the dimorphic prosthecate bacteria (dpb) are alpha-proteobacteria that reproduce in an asymmetric manner rather than by binary fission and are of interest as simple models of development. prior to this work, the only member of this group for which genome sequence was available was the model freshwater organism caulobacter crescentus. here we describe the genome sequence of hyphomonas neptunium, a marine member of the dpb that differs from c. crescentus in that h. neptunium uses its stalk as a re ... | 2006 | 16980487 |