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phylogeny of the genus simonsiella and other members of the neisseriaceae.16s rdna was sequenced from 16 strains of the oral commensal simonsiella and was used to assess relationships between simonsiella strains and other members of the neisseriaceae. in all analyses, simonsiella strains grouped according to established species designations and the mammalian hosts from which they were isolated. the commensals from cats and dogs formed a monophyletic group. the monophyly of the genus simonsiella, however, could be neither supported nor rejected; deep nodes in the trees ...200212148653
the chance identification of unusual-appearing bacteria in bronchoalveolar lavage fluid.background: a well-known indication for the cytologic examination of bronchoalveolar lavage (bal) fluid is the identification of infectious organisms. however, an important distinction must be made as to whether the organisms seen represent a true opportunistic lower respiratory tract infection or a non-pathologic contamination. case: we describe herein the case of a 13-month-old male infant who presented with persistent chest congestion and tracheobronchitis and who underwent bal as part of his ...201222236754
bacterial population dynamics in a reverse-osmosis water purification system determined by fluorescent staining and pcr-denaturing gradient gel electrophoresis.the bacterial population dynamics in an industrial scale reverse-osmosis (ro) water purification system were analyzed by fluorescent staining methods and denaturing gradient gel electrophoresis (dgge). bacterial numbers increased with storage in a tank, and bacterial diversity changed during the water purification process. a dna sequence-based analysis of the major bands on the dgge gel revealed that simonsiella sp. (betaproteobacteria) was abundant in the source water (activated sludge-treated ...200921566369
detection of simonsiella species in the vagina of a bitch in heat. 200616877683
diversity and phylotype consistency of bacteria in the guts of three bee species (apoidea) at an oilseed rape field.the gut of insects may harbour one of the largest reservoirs of a yet unexplored microbial diversity. to understand how specific insects select for their own bacterial communities, the structural diversity and variability of bacteria found in the gut of different bee species was analysed. for three successive years, adults and larvae of apis mellifera ssp. carnica (honey bee), and bombus terrestris (bumble bee), as well as larvae of osmia bicornis (red mason bee) were collected at a flowering oi ...200616423014
phylogenetic analysis of alysiella and related genera of neisseriaceae: proposal of alysiella crassa comb. nov., conchiformibium steedae gen. nov., comb. nov., conchiformibium kuhniae sp. nov. and bergeriella denitrificans gen. nov., comb. nov.a phylogenetic analysis based on 16s rrna gene sequences reveals that alysiella filiformis belongs to the family neisseriaceae. the genus simonsiella is phylogenetically separated by the genera kingella and neisseria. the species simonsiella crassa and a. filiformis show a close phylogenetic relationship, with the 16s rdna sequence similarity and the dna-dna hybridization representing 98.7% and 35%, respectively. therefore, s. crassa should be transferred from the genus simonsiella to the genus ...200515864755
bacterial diversity in worker adults of apis mellifera capensis and apis mellifera scutellata (insecta: hymenoptera) assessed using 16s rrna sequences.high-fidelity pcr of 16s rrna sequences was used to identify bacteria associated with worker adults of the honeybee subspecies apis mellifera capensis and apis mellifera scutellata. an expected approximately 1.5-kb dna band, representing almost the entire length of the 16s rrna gene, was amplified from both subspecies and cloned. ten unique sequences were obtained: one sequence each clustered with bifidobacterium (gram-positive eubacteria), lactobacillus (gram-positive eubacteria), and gluconace ...200314615218
[pure culture of simonsiella spp]. 196314095026
simonsiellaceae fam.nov.with characterization of simonsiella crassa and alysiella filiformis. 196213983561
differentiation of selected members of the family neisseriaceae (alysiella, eikenella, kingella, simonsiella and cdc groups ef-4 and m-5) by carbohydrate fingerprints and selected phenotypic features.on the basis of nucleic acid relationships, the family neisseriaceae consists of the genera neisseria, kingella, simonsiella and of alysiella filiformis, eikenella corrodens, and the cdc groups ef-4 and m-5. differentiation, especially of the new members of the family, by conventional phenotypic characteristics is difficult and in some cases leads to doubtful results. on the other hand, cellular components proved to be suitable for the characterization of bacterial taxa. we investigated the cell ...19947803932
simonsiella filaments isolated from erosive lesions of the human oral cavity.the morphology and cultural characteristics of bacteria of the simonsiella genus isolated from erosions of oral mucosa are reported here. direct microscopic examination of smeared oral swabs and the consequent selection of suitable culturing procedures and media are compulsory for recovering simonsiella microorganisms from clinical specimens in routine work.19846470103
statistical association of dietary components with simonsiella species residing in normal human mouths.members of the genus simonsiella, aerobic, multicellular filamentous gliding bacteria, were detected in swabbings from the palates of 32% of 212 human subjects free of gross oral pathologies. nutritional evaluations for 142 of the subjects showed a significantly greater daily intake among 53 simonsiella carriers for 13 dietary variables, including four fat components, but there was no significantly greater daily intake for any of the carbohydrate components. overall, there was a general excess d ...19854073897
[simonsiella, a giant bacterium: a little-known oral microorganism. microbiological and ultrastructural study]. 19853868423
isolation of simonsiella sp. from a neonate.a member of the genus simonsiella, presumptively identified as s. muelleri, was isolated from a gastric aspirate taken from a neonate 15 min postpartum. the neonate showed a dental cyst and early eruption of teeth, confirmed by mandibular x ray. the morphological features, cultural characteristics, and antimicrobial susceptibility of the isolate are presented.19873571456
cytologic manifestation of an unusual bacterial form, simonsiella species.puzzling rodlike structures overlying benign squamous cells in exfoliative cytologic specimens from the upper gastrointestinal and respiratory tracts were initially considered to be fungi, protozoa, bronchodilator crystals, hemoglobin tactoids or plastic fragments. their morphologic similarity to simonsiella, a gram-negative bacteria frequently found in the oral cavity, was ultimately recognized. further studies of smears and cultures obtained from the oral cavities of the authors and from the a ...19882456653
a stable variant of simonsiella muelleri with unusual colonial and cellular morphology.the unusual morphology and cellular arrangement of a member of the genus simonsiella is described. the organism is characterized by the formation of very long trichomes, which can be greater than 1,000 microns in length.19902307662
a giant bacterial form (simonsiella) seen in oral exfoliative cytology preparations. 19695256745
simonsiella in ulcers in the lip of a dog. 19883344573
dorsal-ventral differentiation in simonsiella and other aspects of its morphology and ultrastructure.the morphology and ultrastructure of the aerobic, gram-negative multicellular-filamentous bacteria of the genus simonsiella were investigated by scanning and transmission electron microscopy. the flat, ribbon-shaped, multicellular filaments show dorsal-ventral differentiation with respect to their orientations to solid substrata. the dorsal surface, orientated away from the substrate, is convex and possesses an unstructured capsule. the ventral surface, on which the organisms adhere and glide, i ...1977879961
deoxyribonucleic acid base composition of simonsiellaceae.the molar percentages of guanine plus cytosine in the dna of 51 strains of simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in cscl. the dna base ratios ranged from 41-55 mole-% guanine plus cytosine. these values fall within the range known for the order cytophagales, the non-fruiting gliding bacteria, and are outside the range of the order myxobacterales, the fruiting myxobacteria. among the strains of the genus simonsiella, four distinct group can be deli ...1977406870
incidence of simonsiella in the oral cavity of dogs.the gliding bacterium simonsiella (cytophagales, simonsiellaceae) was found in palate samples from 66 out of 67 dogs. it is considered a common resident in the oral cavities of dogs.1977886011
isolation, characterization, and numerical taxonomy of simonsiella strains from the oral cavities of cats, dogs, sheep, and humans.forty-nine strains of the gliding prokaryote simonsiella were isolated from the oral cavities of cats (8), dogs (19), sheep (4), and humans (18) in southern california by a direct isolation procedure using a complex serum-enriched medium. the numerical taxonomic analysis (unweighted pair-group method using arithmetric averages) of 57 differential traits for each strain was based on standard bacteriological diagnostic tests and included the molar guanine-plus-cytosine contents of the dna and the ...1978697509
fatty acid composition of simonsiella strains.gas-liquid chromatography of methyl esters of bound fatty acids extracted from the cells of 48 simonsiella strains showed that these aerobic, gliding, multicellular-filamentous bacteria have fatty acid profiles of the pattern considered typical of gram-negative eubacteria. all strains contained predominantly tetradecanoic acid (29.5%), 9-hexadecenoic acid (22.2%), an unidentified acid with an equivalent chain length of approximately 20 carbon atoms (15.8%), and dodecanoic acid (11.4%). discrimin ...1977560178
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