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a phylogenetic analysis of the myxobacteria: basis for their classification.the primary sequence and secondary structural features of the 16s rrna were compared for 12 different myxobacteria representing all the known cultivated genera. analysis of these data show the myxobacteria to form a monophyletic grouping consisting of three distinct families, which lies within the delta subdivision of the purple bacterial phylum. the composition of the families is consistent with differences in cell and spore morphology, cell behavior, and pigment and secondary metabolite produc ...19921384053
nannochelins a, b and c, new iron-chelating compounds from nannocystis exedens (myxobacteria). production, isolation, physico-chemical and biological properties.novel citrate-hydroxamate siderophores, named nannochelins a, b and c, were isolated from the culture broth of the myxobacterium nannocystis exedens strain na e485. the new substances showed weak growth-inhibitory activity against some bacteria and fungi.19921556005
5s rrna sequences of myxobacteria and radioresistant bacteria and implications for eubacterial evolution.5s rrna sequences were determined for the myxobacteria cystobacter fuscus, myxococcus coralloides, sorangium cellulosum, and nannocystis exedens and for the radioresistant bacteria deinococcus radiodurans and deinococcus radiophilus. a dendrogram was constructed by using weighted pairwise grouping based on these and all other previously known eubacterial 5s rrna sequences, and this dendrogram showed differences as well as similarities compared with results derived from 16s rrna analyses. in the ...19902125831
distribution of multicopy single-stranded dna among myxobacteria and related species.multicopy single-stranded dna (msdna) is a short single-stranded linear dna originally discovered in myxococcus xanthus and subsequently found in stigmatella aurantiaca. it exists at an estimated 500 to 700 copies per chromosome (t. yee, t. furuichi, s. inouye, and m. inouye, cell 38:203-209, 1984). we found msdna in other myxobacteria, including myxococcus coralloides, cystobacter violaceus, cystobacter ferrugineus (cbfe17), nannocystis exedens, and nine independently isolated strains of m. xan ...19853932332
nannocystis exedens gen. nov., spec. nov., a new myxobacterium of the family sorangiaceae. 19705429631
pcr-based preparation of 23s rrna-targeted group-specific polynucleotide probes.dna coding for a variable region within domain iii of bacterial 23s rrna was used as the target for group-specific polynucleotide hybridization probes. the corresponding rdna was amplified in vitro by the pcr technique in combination with a pair of primers specific for flanking conserved target sites. the amplified fragments were cloned or used directly as probes. rna probes were generated by in vitro transcription of cloned or amplified rdna. the probes were labeled by incorporating modified nu ...19947524442
diversity of retron elements in a population of rhizobia and other gram-negative bacteria.genetic elements called retrons reside on the chromosome of escherichia coli and the myxobacteria and represent the first reverse transcriptase-encoding element to be found in a prokaryotic cell. all known retrons produce a functionally obscure rna-dna satellite molecule called multicopy single-stranded dna (msdna). we report here the presence of msdna-producing retron elements in a number of new bacterial groups, including strains of the genera proteus, klebsiella, salmonella, nannocystis, rhiz ...19937686549
phylogenetic comparison of retron elements among the myxobacteria: evidence for vertical inheritance.twenty-eight myxobacterial strains, representing members from all three subgroups, were screened for the presence of retron elements, which are novel prokaryotic retroelements encoding reverse transcriptase. the presence of retrons was determined by assaying strains for a small satellite dna produced by reverse transcription called multicopy, single-stranded dna (msdna). an msdna-producing retron appeared to be absent from only one of the strains surveyed. dna hybridization experiments revealed ...19957798147
repetitive sequences found in the chromosome of the myxobacterium nannocystis exedens are similar to msdna: a possible retrotransposition event in bacteria.the first reverse transcriptase (rt) to be found in a prokaryotic cell is encoded by an element called a retron which resides in the chromosome of many different bacteria. in addition, all retrons code for a functionally obscure rna-dna satellite molecule called msdna. msdna is synthesized from an rna template by the retron-encoded rt. an unusual retron element is described here from the myxobacterium nannocystis exedens. this retron does not appear to have a typical rt gene in close proximity ( ...19979157251
isolation of myxobacteria from the marine environment.in an attempt to isolate indigenous marine myxobacteria from coastal samples, we obtained two swarm forming bacteria. both isolates formed cell aggregates which, at least in one isolate, developed to fruiting body-like structures consisting of a mass of myxospore-like cells. the optimum nacl concentrations for their growth were between 2 and 3%, comparable to the nacl concentration of seawater. this growth characteristic strongly suggests that the two isolates are specific marine bacteria. the 1 ...19989868776
the correlation between morphological and phylogenetic classification of myxobacteria.in order to determine whether morphological criteria are suitable to affiliate myxobacterial strains to species, a phylogenetic analysis of 16s rdnas was performed on 54 myxobacterial strains that represented morphologically 21 species of the genera angiococcus, archangium, chondromyces, cystobacter, melittangium, myxococcus, polyangium and stigmatella, five invalid species and three unclassified isolates. the analysis included 12 previously published sequences. the branching pattern confirmed t ...199910425789
nannocystis exedens: a potential biocompetitive agent against aspergillus flavus and aspergillus parasiticus.this study examined the potential for controlling toxigenic aspergillus flavus and aspergillus parasiticus by biological means using a myxobacterium commonly found in soil. the ability of nannocystis exedens to antagonize a. flavus atcc 16875, a. flavus atcc 26946, and a. parasiticus nrrl 3145 was discovered. cultures of aflatoxigenic fungi were grown on 0.3% trypticase peptone yeast extract agar for 14 days at 28 degrees c. when n. exedens was grown in close proximity with an aflatoxigenic mold ...200111456188
characterization of a bioflocculant produced by the marine myxobacterium nannocystis sp. nu-2.the marine myxobacterium strain nu-2, which can grow on high concentrations (up to 7%) of nacl, was isolated from a salt soil sample collected from the coast of the huanghai sea, china. morphological properties and 16s rdna sequence analysis indicated that the isolate is a novel species related to the genus nannocystis. nannocystis sp. nu-2 produced a new kind of flocculating substance in a starch medium with a yield of 14.8 g l(-1). the nu-2 flocculant was composed of 40.3% proteins and 56.5% p ...200212172619
a partial copy of msdna from a new retron element is likely a retrotransposed dna found in the myxobacterium nannocystis exedens.retrons are reverse transcriptase (rt) encoding genetic elements usually located on the chromosome of a wide variety of mostly gram-negative bacteria. here we describe a new retron, designated ne144, found in the chromosome of the myxobacterium nannocystis exedens. this element codes for a 515-amino-acid rt that is most closely related to those found in other myxobacterial retrons. the rt is responsible for the production of a small satellite dna called msdna. this msdna is composed of a 144 bas ...200212459273
plesiocystis pacifica gen. nov., sp. nov., a marine myxobacterium that contains dihydrogenated menaquinone, isolated from the pacific coasts of japan.two strains of a novel myxobacterium (designated sir-1t and shi-1) were isolated from japanese coasts located in the pacific subtropical zone. cells of both strains were gram-negative, rod-shaped and motile by gliding. the strains were chemoheterotrophic and strictly aerobic. they had the common characteristics associated with myxobacteria, such as bacteriolytic action and fruiting-body formation. the characteristic feature of the strains was a nacl growth requirement with an optimum concentrati ...200312656172
exploring the diversity of myxobacteria in a soil niche by myxobacteria-specific primers and probes.the diversity of myxobacteria in a soil niche was explored using culture-dependent and -independent methods. conventional cultivation for bacteriolytic myxobacteria produced six types of myxobacteria, which were identified as two myxococcus spp., two corallococcus spp., a cystobacter sp. and a nannocysts sp. hybridization analysis of the soil bacterial 16s rrna gene library with myxobacteria-specific probes revealed that myxobacteria accounted for less than 1% in the bacterial community. a cysto ...200516156733
biosynthesis of the off-flavor 2-methylisoborneol by the myxobacterium nannocystis exedens. 200717899580
phenylnannolones a-c: biosynthesis of new secondary metabolites from the myxobacterium nannocystis exedens.myxobacteria are gliding bacteria that belong to the delta-proteobacteria and are known for their unique biosynthetic capabilities. among myxobacteria, nannocystis spp. are most closely related to marine myxobacteria and their secondary metabolism has hardly been investigated. phenylnannolones a (1), b (2) and c (3) were obtained from a culture of nannocystis exedens that was isolated from the intertidal region of crete. compound 1 had inhibitory activity toward the abcb1 gene product p-glycopro ...200819040244
the biosynthesis of branched dialkylpyrazines in myxobacteria.the biosynthesis of the volatiles 2,5- and 2,6-diisopropylpyrazine (2 and 3, resp.) released by the myxobacteria nannocystis exedens subsp. cinnabarina (na c29) and chondromyces crocatus (strains cm c2 and cm c5) was studied. isotopically labeled precursors and proposed pathway intermediates were fed to agar plate cultures of the myxobacteria. subsequently, the volatiles were collected by use of a closed loop stripping apparatus (clsa), and incorporation into the pyrazines was followed by gc/ms ...201020860022
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