| novel expression hosts for complex secondary metabolite megasynthetases: production of myxochromide in the thermopilic isolate corallococcus macrosporus gt-2. | abstract: although many secondary metabolites with diverse biological activities have been isolated from myxobacteria, most strains of these biotechnologically important gliding prokaryotes remain difficult to handle genetically. in this study we describe the new fast growing myxobacterial thermophilic isolate gt-2 as a heterologous host for the expression of natural product biosynthetic pathways isolated from other myxobacteria. according to the results of sequence analysis of the 16s rdna, thi ... | 2009 | 19126236 |
| comparative analysis of myxococcus predation on soil bacteria. | predator-prey relationships among prokaryotes have received little attention but are likely to be important determinants of the composition, structure, and dynamics of microbial communities. many species of the soil-dwelling myxobacteria are predators of other microbes, but their predation range is poorly characterized. to better understand the predatory capabilities of myxobacteria in nature, we analyzed the predation performance of numerous myxococcus isolates across 12 diverse species of bact ... | 2010 | 20802074 |
| isolation and identification of myxobacteria from saline-alkaline soils in xinjiang, china. | fifty-eight terrestrial and salt-tolerant myxobacteria were isolated from the saline-alkaline soils collected from xinjiang, china. based on the morphologies and the 16s rrna gene sequences, these isolates were assigned into 6 genera, myxococcus, cystobacter, corallococcus, sorangium, nannocystis and polyangium. all the strains grew better with 1% nacl than without nacl. some myxococcus strains were able to grow at 2% nacl concentration, suggesting that these strains may be particular type of te ... | 2013 | 23936436 |
| approach to analyze the diversity of myxobacteria in soil by semi-nested pcr-denaturing gradient gel electrophoresis (dgge) based on taxon-specific gene. | the genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions. in this study, we developed a semi-nested-pcr-denaturing gradient gel electrophoresis (dgge) strategy to determine the presence and genotypic diversity of myxobacteria in soil. after two rounds of pcr with myxobacteria-specific primers, an 194 bp fragment of mgla, a key gene involved in gliding motility, suitable for dgge was ob ... | 2014 | 25280065 |
| complete genome sequence and comparative genomics of a novel myxobacterium myxococcus hansupus. | myxobacteria, a group of gram-negative aerobes, belong to the class δ-proteobacteria and order myxococcales. unlike anaerobic δ-proteobacteria, they exhibit several unusual physiogenomic properties like gliding motility, desiccation-resistant myxospores and large genomes with high coding density. here we report a 9.5 mbp complete genome of myxococcus hansupus that encodes 7,753 proteins. phylogenomic and genome-genome distance based analysis suggest that myxococcus hansupus is a novel member of ... | 2016 | 26900859 |
| characteristics and living patterns of marine myxobacterial isolates. | the growth, morphology, and life cycle of two marine myxobacterial isolates, halotolerant myxococcus fulvus strain hw-1 and halophilic haliangium ochraceum strain smp-2, were studied as models to determine the living patterns of myxobacteria in the ocean. the growth, morphology, and development of halotolerant strain hw-1 shifted in response to salinity. the optimal seawater concentration for growth of hw-1 was 0 to 80% (salinity, 0.1 to 2.9%), and the strain grew poorly in media with a salinity ... | 2005 | 15933036 |