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autolysis of high-gc isolates of pseudomonas putrefaciens.high-gc isolates of p. putrefaciens undergo extensive autolysis after growth, resulting in a marked decrease in turbidity and the release of high-molecular-weight dna which imparts a high viscosity to culture broths. the native dna released is resistant to attack by the exocellular dnase activity of the culture broths. autolysis is inhibited by a ph of 6.0 and the presence of 0.001 m mg++ or ca++, and is enhanced by elevated ph values and temperatures. this autolytic phenomenon in broth cultures ...19767999
isolation of yersinia enterocolitica-resembling organisms and alteromonas putrefaciens from vacuum-packed chilled beef cuts. 197939056
[pseudomonas putrefaciens isolation from clinical material (author's transl)]. 1978151592
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
[enterobacteria erroneously identified as salmonella in human faeces (author's transl)].to differentiate citrobacter freundii, enterobacter hafniae, proteus mirabilis and alteromonas putrefaciens from salmonella strains isolated in faeces, the author recommends the use of "hajna-kligler" medium, "nitrate-motility-mannitol" medium, "urease-indole-trytophane desaminase" medium and glycerol peptonated water. the lysis of salmonella and e. hafniae strains by specific phages (felix and callow o: 1 salmonella phage; guinee and valkenburg hafnia phage) is emphasized.1977335345
characterization of some fish and shrimp spoiling bacteria.the classification of some important groups of bacteria involved in fish and shrimp spoilage was studied. trimethylamine is produced by pseudomonas putrefaciens, a "non-defined" group resembling ps. putrefaciens, photobacterium spp. and some moraxella-like bacteria. hypoxanthine is produced by the same groups of bacteria except the last named and also by the "typical shrimp spoilers" (presumptive alteromonas). strong off-odours are produced on fresh fish by ps. putrefaciens, dextrose-oxidative p ...1977341802
spoilage of vacuum-packaged dark, firm, dry meat at chill temperatures.the flora of vacuum-packaged dark, firm, dry meat included thred organisms not usually found on vacuum-packaged meat, yersinia enterocolitica, enterobacter liquefaciens, and alteromonas putrefaciens. y. enterocolitica did not affect the meat quality. production of spoilage odors by e. liquefaciens could be prevented by addition of glucose or citrate to the meat. greening of meat could be prevented by addition of glucose or citrate to the meat. greening of meat by a. putrefaciens was not prevente ...1979453817
pseudomonas putrefaciens as a cause of septicemia in humans.septicemia due to pseudomonas putrefaciens was found in an elderly man with chronic leg ulcers. this organism is rarely cultured from human material and has been reported to cause skin and ear infections in only a few patients. its potential for invasiveness is documented in this case for the fourth time.1979489726
pseudomonas putrefaciens as a cause of bacteremia in humans.pseudomonas putrefaciens has been presented as an opportunistic pathogen in a few isolations from humans. this is the first documented report of a local infection of p. putrefaciens complicated by invasion of the blood stream. the organism was twice isolated from the blood culture and once from the wound exudate of a 73-year-old woman hospitalized for extensive ulcerations on the legs. parenteral ampicillin and gentamicin together with local antiseptic treatment led to an uneventful recovery.1978624771
base composition, size and sequence similarities of genoma deoxyribonucleic acids from clinical isolates of pseudomonas putrefaciens.the mean base compositions of dna from 27 strains of pseudomonas putrefaciens, p. rubescens and p. piscicida ranged from 43-4 to 53-2 mol% gc with genome sizes from 3.04 x 10(9) to 4.23 x 10(9) daltons. on the basis of in vitro dna-dna binding, estimated spectrophotometrically from initial renaturation rates, p. putrefaciens strains were heterogenous in the extent to which they shared similar nucleotide sequences, and were divided into four dna homology groups. the dna characteristics of strains ...1978624934
opportunistic infection of chronic skin ulcers with pseudomonas putrefaciens.heavy growth of pseudomonas putrefaciens was isolated repeatedly in mixed culture with other gram-negative rods from chronic ulcers on the extremities of an elderly patient with burnt-out leprosy. treatment was with systemic cotrimoxazole, topical framycetin and general supportive therapy, and the ulcers gradually healed over a period of 4 weeks.1978629878
surgical treatment of fungating lesion of foot due to moraxella phenylpyruvica. case report.we present a case report of a man who had an abscess-like lesion on his left foot. the lesion discharged pus, enlarged, and became thicker, so that we could not differentiate the real toes from the out-growths. moraxella phenylpyruvica and pseudomonas putrefaciens were identified; direct agglutination tests were postive m. phyenylpyruvica. he was given antibiotics and antituberculosis therapy and had topical treatments. the foul odor disappeared with treatment, and the lesions were removed surgi ...1978662952
[alteromonas putrefaciens. 25 strains isolated in madagascar].25 strains of alteromonas putrefaciens, most of them from marine medium, isolated in madagascar, are reported. biochemical characters are studied to class them in 2 subspecies. routinely, alteromonas putrefaciens must be chiefly differentiate with h2s-producing enterobacteriaceae. the ecology is recapitulated.1978747448
volatiles produced by microorganisms isolated from refrigerated chicken at spoilage.volatile components present at spoilage of refrigerated chicken breasts were identified using high-vacuum-low-temperature distillation techniques followed by analysis with combined temperature-programmed gas chromatography and mass spectrometry. a comparison was made of the compounds detected from both irradiated and non-irradiated muscle stored at 2 and 10 degrees c under both aerobic and anaerobic conditions. isolates were randomly selected from the spoiled poultry, identified, and evaluated f ...1976970942
pseudomonas putrefaciens from clinical material.three strains of pseudomonas putrefaciens were isolated from routine clinical specimens at the university hospital, kuala lumpur, malaysia. their cultural and biochemical characteristic, and antibiotic susceptibilities are presented. characteristics of diagnostic value were stressed. two isolates appeared to have played a pathogenic role in chronic otitis media.19761025737
bacteriocin typing of pseudomonas putrefaciens from food, human clinical specimens, and other sources.with the use of 4 bacteriocin donor strains of pseudomonas putrefaciens most low g+c strins were readily distinguished from high g+c strains. two bacteriocin donor strains exhibited autoinhibition when subjected to bacteriocin typing.19751080390
characteristics of weak h2s-producing isolates of pseudomonas putrefaciens from human infections.isolates from human clinical specimens which produced salmon-colored colonies were found to grow at 2c and produce ornthine decarboxylase, dnase, trimethylamine and only slight amounts of h2s. the notably weak ability of such isolates to produce h2s was originally regarded as a negative reaction. the dna base composition of such isolates was found to range from 48.3 to 49.2 moles % gc. these observations, along with bacteriocin typing, resulted in identification of the isolates as pseudomonas pu ...19751083211
strains of pseudomonas putrefaciens from clinical material.eight strains of pseudomonas putrefaciens have been received from among 466 strains of pseudomonas submitted to the computer trials laboratory for identification over the last eight years. two of the strains of p. putrefaciens from patients with otitis media and otitis externa respectively appear to have played a pathogenic role. the biochemical characteristics of these eight strains were compared with those of seven culture collection strains.19751127110
respiratory colonization with pseudomonas putrefaciens after near-drowning in salt water.pseudomonas putrefaciens, a marine organism infrequently found in human culture material, was repeatedly isolated from the sputum of a patient with pneumonia during a three-week period following a salt-water drowning accident. similar organisms were found in the water at the site of the accident in boston, and at ocean bathing beaches on nearby martha's vineyard.19751163489
sequence of the gene encoding flavocytochrome c from shewanella putrefaciens: a tetraheme flavoenzyme that is a soluble fumarate reductase related to the membrane-bound enzymes from other bacteria.flavocytochrome c from the gram-negative, food-spoiling bacterium shewanella putrefaciens is a soluble, periplasmic fumarate reductase. we have isolated the gene encoding flavocytochrome c and determined the complete dna sequence. the predicted amino acid sequence indicates that flavocytochrome c is synthesized with an n-terminal secretory signal sequence of 25 amino acid residues. the mature protein contains 571 amino acid residues and consists of an n-terminal cytochrome domain, of about 117 r ...19921333793
contaminated marine wounds--the risk of acquiring acute bacterial infection from marine recreational beaches.an animal model was used to determine the potential for causing wound infections of bacteria isolated from marine recreational beaches in hong kong. water samples were characterized physically, chemically and bacteriologically and used to inoculate artificially-induced wounds in rats. morbidity and mortality correlated significantly (p < 0.01) with macconkey plate counts and faecal coliform counts (membrane filtration) and inversely with salinity of the water. the majority of deaths were due to ...19921447057
peritonitis caused by pseudomonas putrefaciens in patients undergoing continuous ambulatory peritoneal dialysis.three cases of peritonitis caused by pseudomonas putrefaciens in patients undergoing continuous ambulatory peritoneal dialysis are described. in two cases asymptomatic colonization of the dialysate preceded overt infection. all patients responded successfully to standard antibiotic therapy with gentamicin or ofloxacin. this is the first report of peritonitis caused by p. putrefaciens.19921571453
localization of cytochromes to the outer membrane of anaerobically grown shewanella putrefaciens mr-1.in gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique problem: they must somehow establish an electron transport link across the outer membrane between large particulate metal oxides and the electron transport chain in the cytoplasmic ...19921592800
a novel lipopolysaccharide-binding hemagglutinin isolated from hemocytes of the solitary ascidian, halocynthia roretzi: it can agglutinate bacteria.a hemagglutinin was isolated from hemocytes of the ascidian, halocynthia roretzi, by a procedure including extraction and ion-exchange chromatography on cm-cellulose. the molecular weight of the hemagglutinin was estimated to be 120,000 by gel filtration. it was resistant to acid treatment but sensitive to alkali or heat treatment. the hemagglutinating activity was inhibited by heparin, chondroitin sulfate, and lipopolysaccharide (lps), but not by mono- and disaccharides such as n-acetyl-galacto ...19911904830
the role of motile aeromonads in the fish disease, ulcerative disease syndrome (uds).ulcerative disease syndrome (uds) is an epizootic fish disease characterized by the presence of severe, open dermal ulcers on the head, midbody, and dorsal regions of the fish. aeromonas hydrophila and a. sobria were recovered more often from uds fish than other bacteria from the genera vibrio, alteromonas and plesiomonas. representative isolates of a. hydrophila, a. sobria, v. anguillarum, v. vulnificus, alteromonas putrefaciens, and p. shigelloides taken from uds and healthy fish were assayed ...19912044697
microbial sensor system for nondestructive evaluation of fish meat quality.a microbial sensor system consisting of the bacterium (alteromonas putrefaciens) immobilized within membranes, a flow cell, an oxygen electrode, peristaltic pumps, a buffer tank, a thermostatically controlled bath and a recorder, was constructed for the nondestructive quality evaluation of bluefin tuna. the chemical compounds on fish meat surfaces which are the indicators of fish meat quality were rapidly determined by using the proposed sensor system. fish meat quality was determined from the r ...19912049168
pseudomonas putrefaciens bacteraemia in a peritoneal dialysis patient. 19912057122
complete nucleotide sequences of seven eubacterial genes coding for the elongation factor tu: functional, structural and phylogenetic evaluations.the nucleotide sequences of cloned genes coding for the elongation factor tu of seven eubacteria have been determined. these genes were from anacystis nidulans, bacillus subtilis, bacteroides fragilis, "deinonema" spec., pseudomonas cepacia, shewanella putrefaciens and streptococcus oralis. the primary structures of the genes were compared to the available sequences of prokaryotic elongation factors tu and eukaryotic elongation factors 1 alpha. a conservation profile was determined for homologou ...19902110445
[isolation of alteromonas putrefaciens from secondary-recovery water in the petroleum industry].eight strains obtained from injection water for secondary recovery of oil were isolated and characterized as alteromonas. this species has been reported in a relatively great number in the system, after its treatment with cresylic acid (used as antibacterial agent). it has the property of forming important viscous sediments which are related to biofouling of the injection system.19902287714
classification of the spoilage flora of fish, with special reference to shewanella putrefaciens.one hundred and fifty-nine gram-negative strains isolated from refrigerated fish, taken from the baltic sea or swedish inland waters, together with 32 reference strains of shewanella, pseudomonas, aeromonas and alcaligenes, were phenotypically classified using 124 unit characters. data were processed by the simple matching (ssm) and jaccard (sj) coefficients, and unweighted pair group algorithm with arithmetic averages. fourteen clusters were defined at the 75% sj similarity level which correspo ...19902391295
the bacteriology of fresh and spoiling lake victorian nile perch (lates niloticus).a total of 177 bacterial cultures isolated from lake victorian nile perch (lates niloticus) were investigated. the flora on newly caught nile perch consisted of organisms belonging to the genera moraxella, alcaligenes, acinetobacter, pseudomonas, aeromonas, micrococcus and other gram-positive organisms. 39% were identified as gram-positive species and 61% were negative in the gram-reaction. three cultures out of 53 investigated caused weak rotten off-odours in sterile fish broth and one culture, ...19902397157
volatile compounds associated with microbial growth on normal and high ph beef stored at chill temperatures.volatile compounds produced by pseudomonas fragi and mixed, natural floras on beef of normal ph (5.5-5.8; glucose greater than 1500 micrograms/g) and high ph (6.3-6.8; glucose less than 10 micrograms/g) included a range of alkyl esters and a number of sulphur-containing compounds including dimethylsulphide but not hydrogen sulphide. production of the last was a property common to the other gram-negative organisms tested viz. hafnia alvei, enterobacter agglomerans, serratia liquefaciens, alteromo ...19892753835
[an electron microscopic study on the ultrastructure and enzyme cytochemistry of pseudomonas putrefaciens].in this paper, pta staining, ultrathin sectioning and electron microscopic enzyme cytochemistry techniques were used to study the ultrastructure, as well as reactivity and localization of catalase and peroxidase in ps. putrefaciens. its ultrastructure is similar to that of other gram-negative bacilli. catalase is distributed in the cytoplasm, while peroxidase localized at cytoplasmic membrane and mesosomes.19892781788
the recent evolutionary origin of the phenylalanine-sensitive isozyme of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase in the enteric lineage of bacteria.evolutionary events that generated the three regulatory isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthase present in contemporary strains of escherichia coli have been proposed recently [ahmad et al. (1986) j bacteriol 165:146-154]. the phylogenetic subdivision of gram-negative prokaryotes studied (superfamily b) includes enteric bacteria, an oceanospirillum cluster, pseudomonad group i (e.g., pseudomonas aeruginosa), pseudomonad group v (e.g., xanthomonas), and the acineto ...19872888901
pseudomonas putrefaciens bacteremia.pseudomonas putrefaciens is an unusual cause of human disease. since 1978 only five cases of bacteremia due to this organism have been reported. within 12 recent months four cases of bacteremia due to p. putrefaciens were seen - two occurred in patients with chronic infections of a lower extremity, one in a patient with neutropenia, and one in a patient with fulminant septicemia and disseminated intravascular coagulation. two of the patients had prostheses; in neither case did prosthetic infecti ...19892916100
omphalitis and peritonitis in a young west indian manatee (trichechus manatus).mortality data for the west indian manatee (trichechus manatus) indicates that from 1979 to 1984 16% of the recorded deaths involved young juveniles. necropsy of a young manatee from the west coast of florida revealed an active infection of the umbilical area (omphalitis) extending down the umbilical artery and veins. a generalized peritonitis was present. bacterial cultures revealed streptococcus faecium, plesiomonas shigelloides, pseudomonas putrefaciens and escherichia coli.19873682102
pseudomonas putrefaciens as a cause of infection in burn patient. 19853833725
quantitative and qualitative aspects of bacterial flora of karachi coastal water shrimp (penaeus merguiensis and metapenaeus monoceros).bacterial counts were made over a period of two years of two important commercial shrimp varieties of karachi coastal waters. bacteria were also isolated and identified. total number of bacteria were found to be remarkably equal at 37 degrees, 30 degrees and 25 degrees c. aerobic plate count of penaeus merguiensis at 37 degrees c ranged from 1.2 x 10(5) to 6.0 x 10(7) cfu/g (median 1.8 x 10(6) cfu/g), and were predominantly vibrio, micrococcus, pseudomonas, staphylococcus, bacillus, and flavobac ...19853913252
dissimilatory nitrate reduction to nitrate, nitrous oxide, and ammonium by pseudomonas putrefaciens.the influence of redox potential on dissimilatory nitrate reduction to ammonium was investigated on a marine bacterium, pseudomonas putrefaciens. nitrate was consumed (3.1 mmol liter-1), and ammonium was produced in cultures with glucose and without sodium thioglycolate. when sodium thioglycolate was added, nitrate was consumed at a lower rate (1.1 mmol liter-1), and no significant amounts of nitrite or ammonium were produced. no growth was detected in glucose media either with or without sodium ...19854083881
volatile compounds produced in sterile fish muscle (sebastes melanops) by pseudomonas putrefaciens, pseudomonas fluorescens, and an achromobacter species.volatile compounds produced by pseudomonas putrefaciens, p. fluorescens, and an achromobacter species in sterile fish muscle (sebastes melanops) were identified by combined gas-liquid chromatography and mass spectrometry. compounds produced by p. putrefaciens included methyl mercaptan, dimethyl disulfide, dimethyl trisulfide, 3-methyl-1-butanol, and trimethylamine. with the exception of dimethyl trisulfide, the same compounds were produced by an achromobacter species. methyl mercaptan and dimeth ...19734199335
correlation of dna base composition and metabolism of pseudomonas putrefaciens isolates from food, human clinical specimens, and other sources. 19724338003
pseudomonas putrefaciens isolates from clinical specimens.a total of 109 cultures of pseudomonas putrefaciens isolated from clinical specimens were studied. the cultures were separated into two groups. the majority of the group 1 isolates, comprising 31 cultures, were characterized by (i) growth in plain nutrient broth, but no growth in broth supplemented with nacl at concentrations of 7% and above, (ii) no growth on salmonella-shigella (ss) agar, and (iii) production of acid from the carbohydrates, sucrose, maltose, arabinose, and dextrin. most group ...19724565638
hydrogen sulfide production by pseudomonas putrefaciens in shrimp experimentally packed in nitrogen.shrimp refrigerated in a nitrogen atmosphere develop off-odors not typical of normal spoilage. investigations of this phenomenon showed that hydrogen sulfide developed in the headspace gas, and a large percentage of the microbial population present on the shrimp stored in nitrogen was capable of hydrogen sulfide production, in contrast to the flora on shrimp stored in air. the predominant hydrogen sulfide-producing organism, pseudomonas putrefaciens, was present in low numbers on fresh shrimp bu ...19744596748
detection and incidence of specific species of spoilage bacteria on fish. ii. relative incidence of pseudomonas putrefaciens and fluorescent pseudomonads on haddock fillets.pseudomonas putrefaciens has been found to constitute one of the major species of spoilage bacteria on haddock fillets. the initial population of this organism on fillets of high bacterial quality is uniformly below 4% and most frequently no greater than 1%. during refrigerated storage, the organism increases at a more rapid rate than the total psychrophilic population, comprising 50 to 90% of the total population when the total count exceeds 10(6)/g of tissue. fluorescent pseudomonads were show ...19684881956
comparison of methods of extracting intracellular proteases from bacteria.five commonly used methods of disintegrating bacterial cells were compared by use of bacillus subtilis, pseudomonas putrefaciens, and streptococcus durans as the test organisms. these methods were: (i) sonic treatment, (ii) grinding, (iii) freezing and thawing, (iv) acetone-powder, and (v) toluene. sonic treatment and grinding yielded more protein in the cell-free extracts than did the other methods. likewise, the protease activities (micrograms of tyrosine liberated per milliliter of extract) o ...19654956035
bacteriophages of psychrophilic pseudomonads. ii. host range of phage active against pseudomonas putrefaciens. 19695309906
detection and incidence of specific species of spoilage bacteria on fish. i. methodology.the ability of pseudomonas putrefaciens to form h(2)s was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of peptone-iron agar. subsurface colonies appear intensely black, whereas surface colonies are black or gray. a highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms f ...19685726148
extracellular nuclease activity of fish spoilage bacteria, fish pathogens, and related species.the production of extracellular deoxyribonuclease and ribonuclease by 23 marine and 3 dairy strains of pseudomonas putrefaciens, 15 strains of fish-pathogenic fluorescent pseudomonads, 38 strains of fluorescent pseudomonads isolated from haddock, and 34 related organisms was determined by an agar plate method. all strains of p. putrefaciens produced both deoxyribonuclease and ribonuclease. of the other 87 organisms examined, 26.5% produced ribonuclease and 14.5% produced deoxyribonuclease. all o ...19695797936
growth of non-campylobacter, oxidase-positive bacteria on selective campylobacter agar.a total of 67 oxidase-positive, gram-negative bacteria were tested for growth on selective campylobacter agar (blaser formulation, bbl microbiology systems, cockeysville, md.) at 42 degrees c under microaerophilic conditions. although the growth of most of these bacteria was prevented, all strains of achromobacter xylosoxidans, pseudomonas aeruginosa, pseudomonas putrefaciens, pseudomonas alcaligenes, and pseudomonas pseudoalcaligenes grew as well as campylobacter fetus subsp. jejuni.19826179961
[antibacterial activities of cefotiam against various pathogens isolated from clinical materials (author's transl)].bacteriological evaluation was made on cefotiam (ctm), a new broad-spectrum cephalosporin antibiotic, and the following results wer obtained. 1) ctm has shown very potent antibacterial activities against staphylococcus aureus, escherichia coli, edwardsiella tarda, citrobacter intermedius, salmonella, klebsiella, proteus mirabilis, proteus rettgeri, proteus inconstans, yersinia enterocolitica, aeromonas hydrophila, plesiomonas shigelloides and pseudomonas putrefaciens. 2) streptococcus faecalis, ...19826285018
effect of temperature on the microbial flora of herring fillets stored in air or carbon dioxide.the microbial development on fillets of herring from the baltic sea was studied at temperatures from 0-15 c in air or 100% carbon dioxide (96-100% co2). the shelf-life of the fillets , defined as the time for the 'total aerobic count' to reach 1 x 10(7) c.f.u./g increased with decreased temperature from 1 d at 15 degrees c to 7 d at 0 degrees c (air). the corresponding values in co2 were 3 d and 33 d, respectively. the initial flora of the herring fillets was dominated by alteromonas putrefacien ...19846427171
trimethylamine oxide respiration of alteromonas putrefaciens ncmb 1735: na+-stimulated anaerobic transport in cells and membrane vesicles.alteromonas putrefaciens ncmb 1735 required the presence of nacl for anaerobic growth with serine, cysteine, and formate as substrate and trimethylamine oxide ( tmao ) as external electron acceptor. when lactate was substrate, the organism grew equally well in the absence of nacl. anaerobic uptake of glutamate, aspartate, serine, cysteine, and lactate in resting cells was strongly stimulated with nacl, and cytoplasmic membrane vesicles energized by electron transfer from formate to tmao displaye ...19846430228
pseudomonas putrefaciens meningitis. 19836663089
amino acid and lactate catabolism in trimethylamine oxide respiration of alteromonas putrefaciens ncmb 1735.the nonfermentative alteromonas putrefaciens ncmb 1735 grew anaerobically in defined media with trimethylamine oxide as external electron acceptor. all amino acids tested, except taurine and those with a cyclic or aromatic side chain, were utilized during trimethylamine oxide-dependent anaerobic growth. lactate, serine, and cysteine (which are easily converted to pyruvate) and glutamate and aspartate (which are easily converted to tricarboxylic acid cycle intermediates) were metabolized at the f ...19846742826
headspace analysis of volatile metabolites of pseudomonas aeruginosa and related species by gas chromatography-mass spectrometry.gas chromatographic-mass spectrometric analysis of headspace volatiles was performed on cultures of 11 strains of pseudomonas aeruginosa and 1 strain each of pseudomonas cepacia, pseudomonas putida, pseudomonas putrefaciens, pseudomonas fluorescens, and pseudomonas maltophilia. all strains of pseudomonas aeruginosa produced a distinctive series of odd-carbon methyl ketones, particularly 2-nonanone and 2-undecanone, and 2-aminoacetophenone. the other strains failed to produce 2-aminoacetophenone. ...19806775012
[pseudomonas putrefaciens isolated from clinical specimen]. 19826816859
relative incidence of alteromonas putrefaciens and pseudomonas putrefaciens in ground beef.of 65 h(2)s-producing isolates from seven samples of ground beef, 64 were found to be alteromonas putrefaciens. isolates of pseudomonas putrefaciens were not encountered. the mean guanine-plus-cytosine content of dnas from 10 of the representative isolates, obtained from thermal denaturation determinations, was 46.5 +/- 1.0 mol%, which is consistent with the designation a. putrefaciens.19836847186
isolation of pseudomonas putrefaciens in intra-abdominal sepsis.we report the isolation of pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. in all three cases, p. putrefaciens was isolated in mixed culture with enteric bacteria.19836874910
pseudomonas putrefaciens and group a beta-hemolytic streptococcus septicemia.pseudomonas putrefaciens was isolated twice in blood cultures from which group a beta-hemolytic streptococci were concurrently isolated from a moribund woman. only once previously has p putrefaciens bacteremia been reported. to our knowledge, we are reporting the first blood isolate of p putrefaciens, albeit with group a beta-hemolytic streptococci, associated with fatal septicemia.19807002085
numerical taxonomy of psychrotrophic pseudomonads.the taxonomy of 218 psychrotrophic pseudomonad strains (200 field strains from meat and 18 type and reference strains) was numerically studied by 174 biochemical and physiological tests. all strains were gram-negative rods, oxidative positive and motile by means of one or more polar flagella. the strains clustered into 15 groups, of which 9 were regarded as major clusters. the major clusters were designated as pseudomonas fragi (112 strains), p. fluorescens biotype iii (7 strains), p. fluorescen ...19827119735
lipid composition and chemotaxonomy of pseudomonas putrefaciens (alteromonas putrefaciens).the major polar lipids in cells of pseudomonas putrefaciens ncib 10472 grown on nutrient agar were phosphatidylethanolamine, phoisphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. an additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. the wall lipids from broth cultures of ...19807441198
modelling of microbial activity and prediction of shelf life for packed fresh fish.prediction of shelf life based on growth of specific spoilage organisms (sso) in model substrates was studied. the effect of co2 on the growth kinetics for photobacterium phosphoreum and shewanella putrefaciens was quantified and modelled. results showed that microbial spoilage of packed cod stored with various concentrations of co2 was accurately predicted from the effect of co2 on p. phosphoreum grown in model substrates. the short shelf life extensions previously reported for packed cod there ...19957488526
qualitative and quantitative characterization of spoilage bacteria from packed fish.the large cells recently suggested to be responsible for spoilage of packed cod, have been identified as photobacterium phosphoreum. the spoilage activity of these cells, of shewanella putrefaciens and of other microorganisms isolated form spoiled packed cod has been studied. both qualitative and quantitative tests were used for characterization of the microbial spoilage activity. the importance of the different groups of microorganisms was evaluated by comparison of microbial spoilage activity ...19957488527
occurrence of a furan fatty acid in marine bacteria.a fatty acid containing a furan ring was detected in the cellular lipids of marine bacteria, shewanella putrefaciens, marinomonas comunis, enterobacter agglomerans, pseudomonas fluorescens, etc., which were isolated from the intestinal liquor of fishes. analytical data indicated that the fatty acid was 10,13-epoxy-11-methyloctadeca-10, 12-dienoic acid. therefore, we propose that furan fatty acids detected in marine fish are derived not only from marine plants but also from intestinal bacteria of ...19957548190
molecular cloning, expression in streptomyces lividans, and analysis of a gene cluster from arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein.the arthrobacter simplex gene coding for 3-ketosteroid-delta 1-dehydrogenase, a key enzyme in the degradation of the steroid nucleus, was cloned in streptomyces lividans. nucleotide sequence analysis revealed that the gene for 3-ketosteroid-delta 1-dehydrogenase (ksdd) is clustered with at least two more genes possibly involved in steroid metabolism. upstream of ksdd, we found a gene, ksdr, encoding a hypothetical regulatory protein that shows homologies to kdgr, the negative regulator of pectin ...19957596291
shewanella (pseudomonas) putrefaciens bacteremia.shewanella (pseudomonas) putrefaciens is a rare pathogen in humans, and to our knowledge only 13 cases of s. putrefaciens bacteremia have ever been reported in the literature. in this retrospective study we describe 28 cases of s. putrefaciens bacteremia: 16 in premature and 1-day-old neonates, 9 in adults, and 3 in children younger than 1 year of age. all the babies presented with respiratory distress and/or pneumonia. six of the adults had associated traumatic lesions of the lower extremity, a ...19957620019
antibacterial effect of protamine assayed by impedimetry.impedimetric measurements were used to assay the antibacterial effect of protamine. a good linear correlation between the impedance detection time and the initial cell counts was obtained (r = 0.99, n = 2). as basic peptides may cause clumping of cells, this correlation curve was used when estimating the cell number after protamine treatment, rather than colony counts. protamine from salmon killed growing gram-positive bacteria and significantly inhibited growth of gram-negative bacteria in tryp ...19957730206
the fnr family of transcriptional regulators.homologues of the transcriptional regulator fnr from escherichia coli have been identified in a variety of taxonomically diverse bacterial species. despite being structurally very similar, members of the fnr family have disparate regulatory roles. those from shewanella putrefaciens, pseudomonas aeruginosa, pseudomonas stutzeri and rhodopseudomonas palustris are functionally similar to fnr in that they regulate anaerobic respiration or carbon metabolism. four rhizobial proteins (from rhizobium me ...19947747934
[a simple method for differentiation of hydrogen sulfide-producing bacteria by the ph-dependent edta-sensitivity test].the ph-dependent edta-sensitivity test was performed to differentiate several strains of bacteria forming black colonies by the production of hydrogen sulfide on tcbs (thiosulfate-citrate-bile salt-sucrose agar) medium (tentatively designated as hydrogen-sulfide production bacteria). two halotorelant strains of 16 hydrogen sulfide-producing strains showed the same bacteriological properties and isoprenoid quinone type as did a reference strain of proteus mirabilis and were classified into the ed ...19947830320
spectroscopic and kinetic studies of the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400.electron paramagnetic resonance (epr) and magnetic circular dichroism (mcd) spectroscopic studies were carried out on the tetraheme flavocytochrome c from shewanella putrefaciens ncimb400. the epr spectrum reveals two sets of g-values--gz = 2.93, gy = 2.28, and gx = 1.51; and gz = 3.58--and the mcd spectrum shows a charge-transfer band at 1510 nm. these data combined show that all four hemes are low spin and have a nitrogenous sixth ligand. sequence comparisons with other tetraheme cytochromes, ...19957742319
the 16s rrna sequence and genome sizing of tributyltin resistant marine bacterium, strain m-1.the 16s rrna of the tributyltin resistant marine bacterium, strain m-1, was partly sequenced to confirm the taxonomic status. the results indicated that this bacterium should be classified under the genus alteromonas, instead of a previous report in which this strain was identified as a vibrio. the genome size of this strain was also measured by pulsed field gel electrophoresis (pfge) using a contoureclamped homogeneous electric field. the strain was found to contain a genome size of 2,240 kilo ...19947512189
design and application of rrna-targeted oligonucleotide probes for the dissimilatory iron- and manganese-reducing bacterium shewanella putrefaciens.a 16s rrna-targeted oligonucleotide probe specific for the iron (fe3+)- and manganese (mn4+)-reducing bacterium shewanella putrefaciens was constructed and tested in both laboratory- and field-based hybridization experiments. the radioactively labeled probe was used to detect s. putrefaciens in field samples collected from the water column and sediments of oneida lake in new york and its major southern tributary, chittenango creek. s. putrefaciens was quantified by (i) hybridization of the probe ...19937506899
a biochemical study of the intermediary carbon metabolism of shewanella putrefaciens.cell extracts were used to determine the enzymes involved in the intermediary carbon metabolism of several strains of shewanella putrefaciens. enzymes of the entner-doudoroff pathway (6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase) were detected, but those of the embden-meyerhof-parnas pathway were not. while several tricarboxylic acid cycle enzymes were present under both aerobic and anaerobic conditions, two key enzymes (2-oxoglutarate dehydrogenase and pyruvate ...19948195102
sequence and genetic characterization of etra, an fnr analog that regulates anaerobic respiration in shewanella putrefaciens mr-1.an electron transport regulatory gene, etra, has been isolated and characterized from the obligate respiratory bacterium shewanella putrefaciens mr-1. the deduced amino acid sequence of etra (etra) shows a high degree of identity to both the fnr of escherichia coli (73.6%) and the analogous protein (anr) of pseudomonas aeruginosa (50.8%). the four active cysteine residues of fnr are conserved in etra, and the amino acid sequence of the dna-binding domains of the two proteins are identical. furth ...19938253682
[alteromonas putrefaciens. taxonomy and cultivation (author's transl)].the morphology and biochemical activity of pseudomonas/alteromonas putrefaciens is described. features indicating the uncertain taxonomic position of the organism are discussed, and it is pointed out why a. putrefaciens does not fit into neither pseudomonas nor alteromonas, but should be considered as a separate unit or group. table i shows criteria which can be used for differentiation between a. putrefaciens and the genera pseudomonas, alcaligenes and alteromonas. essential characteristics are ...19817322860
[isolation and identification of a strain of pseudomonas putrefaciens]. 19817280316
inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish.the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ...19938357253
production and specificity of poly- and monoclonal antibodies raised against shewanella putrefaciens.polyclonal antibodies were raised in rabbits and mice against shewanella putrefaciens. murine monoclonal antibodies were produced against the type strain (atcc 8071) as well as wild type strains isolated from fish products. the specificities of four polyclonal and 12 monoclonal antibodies were tested by dot-blotting, an indirect and a competitive elisa against 16 gram-negative strains; including six strains of s. putrefaciens and one strain of pseudomonas rubescens (nc 10695). all polyclonal ant ...19938486552
purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans.the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ...19958586617
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
effect of temperature on an obligately psychrophilic phage-host system of pseudomonas putrefaciens. 19724537090
characteristics of three phages infectious for psychrophilic fishery isolates of pseudomonas putrefaciens. 19724537085
shewanella alga bacteremia in two patients with lower leg ulcers.the first danish cases of shewanella alga bacteremia in two patients with chronic lower leg ulcers are reported. both patients were admitted to the hospital during the same month of a very warm summer and had been exposed to the same marine environment, thereby suggesting the same source of infection. both patients survived; however, one of them had extensive myonecrosis, while the other patient had an uncomplicated course. the strains were initially believed to be shewanella putrefaciens on the ...19968783706
spoilage bacteria of fresh broiler chicken carcasses.studies were conducted to identify the bacteria responsible for spoilage of fresh broiler chicken carcasses and to characterize the off-odors these bacteria produce. broiler carcasses were collected from processing plants in the northeast georgia area, the southeastern u.s., arkansas, california, and north carolina. the carcasses were allowed to spoil under controlled conditions at 3 c and spoilage bacteria were isolated. each spoilage bacterium was separately inoculated into a sterile chicken s ...19958825595
[characteristics of phenotypes of alteromonas putrefaciens. study of 123 strains].the phenotypic characteristics of 124 strains of alteromonas putrefaciens, a bacterium that produces large quantities of hydrogen sulfide, were studied. the strains were isolated from man, warm and cold-blooded animals (especially fish and shellfish), marine and fresh waters. two phenetic groups were differentiated: group a strains grew at 42 degrees c, but not at 4 degrees c, tolerated 6 p. cent nacl and did not utilize glucose or maltose, unlike group b strains. each group could be divided int ...19854091319
glucose, the key substrate in the microbiological changes occurring in meat and certain meat products.the literature dealing with the role of glucose in the microbiological changes of meat and certain meat products is reviewed. discussion is centered on two aspects. first, glucose plays a part in the selection of the dominant spoilage organisms, pseudomonas fragi, ps. lundensis, and ps. fluorescens, on red meat stored aerobically under chill (2-7 degrees c) conditions. it is concluded that the pseudomonads flourish because they convert glucose to the less commonly used substrate, gluconate. the ...19883293613
growth and activity of shewanella putrefaciens isolated from spoiling fish.a total of 101 cultures of h2s-producing organisms isolated from spoiling cod was studied. all cultures were identified as shewanella putrefaciens. two groups were distinguished on the basis of pattern of trimethylamine oxide reduction determined by conductance measurement, generation time at 25 degrees c and salt tolerance. the s. putrefaciens cultures were further characterized in a number of model experiments in order to examine the spoilage activity under various conditions. a good correlati ...19892641275
pseudomonas putrefaciens bacteremia associated with shellfish contact. 19902294774
changes in cell morphology of listeria monocytogenes and shewanella putrefaciens resulting from the action of protamine.protamine, which is an antibacterial basic peptide, was shown to alter the cell morphology of listeria monocytogenes and shewanella putrefaciens. atomic force microscopy revealed that protamine smoothed the surface of cells, formed holes in the cell envelope, and caused fusion of s. putrefaciens cells. immunoelectron microscopy of protamine-treated cells of both l. monocytogenes and s. putrefaciens showed great damage to the cell wall and condensation of the cytoplasm. respiration of the cells w ...19968975598
influence of respiratory substrate on the cytochrome content of shewanella putrefaciens.shewanella putrefaciens can use trimethylamine oxide (tmao) as electron acceptor under anoxic conditions. the associated cytochromes induced during growth under various respiratory conditions have been separated by liquid chromatography (deae sepharose cl6b) and sds-page and characterized spectrophotometrically and by redox potentiometry. two major low potential cytochromes and at least three minor low potential cytochromes, likely to be involved in tmao reduction, were found. no cytochrome spec ...19902210338
respiration-linked proton translocation coupled to anaerobic reduction of manganese(iv) and iron(iii) in shewanella putrefaciens mr-1.an oxidant pulse technique, with lactate as the electron donor, was used to study respiration-linked proton translocation in the manganese- and iron-reducing bacterium shewanella putrefaciens mr-1. cells grown anaerobically with fumarate or nitrate as the electron acceptor translocated protons in response to manganese (iv), fumarate, or oxygen. cells grown anaerobically with fumarate also translocated protons in response to iron(iii) and thiosulfate, whereas those grown with nitrate did not. aer ...19902172208
protamine-induced permeabilization of cell envelopes of gram-positive and gram-negative bacteria.the inhibitory effect of the cationic peptide protamine on listeria monocytogenes, escherichia coli, and shewanella putrefaciens has been studied in detail. the addition of protamine (10 to 1,000 micrograms/ml) resulted in inhibition of oxygen consumption after less than 1 min and loss of intracellular carboxyfluorescein and atp after 2 to 5 min. maximum antibacterial activity was reached at alkaline ph and in the absence of divalent cations. the efficient permeabilization of cell envelopes of b ...19979055431
inhibitory effect of halocyamine, an antimicrobial substance from ascidian hemocytes, on the growth of fish viruses and marine bacteria.halocyamine a, an antimicrobial substance isolated from hemocytes of the solitary ascidian halocynthia roretzi, inhibited in vitro the growth of fish rna viruses (infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus). pretreatment of rna virus with halocyamine a reduced the infectivity of the virus toward host cells. the growth of marine bacteria, achromobacter aquamarinus and pseudomonas perfectomarinus, was also inhibited by halocyamine a but that of alteromonas put ...19902121517
cellulitis due to pseudomonas putrefaciens: possible production of exotoxins.pseudomonas putrefaciens has been described as a rare cause of both lower-limb cellulitis and septicemic illness with significant morbidity. we report a case of p. putrefaciens infection in a patient with refractory lower-limb cellulitis and ulceration complicated by thrombocytopenia, hypotension, and mental obtundation in the apparent absence of bacteremia. this scenario raises the possibility of significant production of exotoxins by p. putrefaciens in vivo.19911925285
novel mercury resistance determinants carried by incj plasmids pmerph and r391.hgcl2 resistance (hgr) in a strain of pseudomonas putrefaciens isolated from the river mersey was identified as plasmid-borne by its transfer to escherichia coli in conjugative matings. this plasmid, pmerph, could not be isolated and was incompatible with the chromosomally integrated incj hgr plasmid r391. pmerph and r391 both express inducible, narrow-spectrum mercury resistance and detoxify hgcl2 by volatilization. the cloned mer determinants from pmerph (psp100) and r391 (psp200) have very si ...19911886614
biosynthesis of furan fatty acids (f-acids) by a marine bacterium, shewanella putrefaciens.a mutant derived from shewanella putrefaciens 8cs7-4 treated with n-methyl-n'-nitro-n-nitrosoguanidine was found to produce 15-20 mg of a furan fatty acid (f-acid), 10,13-epoxy-11-methyloctadeca-10,12-dienoic acid (f18), per liter of growth medium (10-15% of total fatty acids). capillary gas chromatography-mass spectrometry and proton nuclear magnetic resonance analysis of the fatty acid methyl esters of the mutant revealed the presence of other f-acids, 8,11-epoxy-9-methylhexadeca-8,10-dienoic ...19979219910
expression of the eicosapentaenoic acid synthesis gene cluster from shewanella sp. in a transgenic marine cyanobacterium, synechococcus sp.the eicosapentaenoic acid (epa) synthesis gene cluster isolated from a marine bacterium, shewanella putrefaciens strain scrc-2738, was cloned and expressed in the marine cyanobacterium synechococcus sp. a broad-host-range cosmid vector, pjrd215 (10.2 kb, smr kmr), was used to clone a 38 kb insert, pepa, containing the epa synthesis gene cluster, creating plasmid pjrdepa (approx. 48 kb). this plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10(-7). cyanobacterial transco ...19979274025
effects of nitrate and nitrite on dissimilatory iron reduction by shewanella putrefaciens 200.the inhibitory effects of nitrate (no3-) and nitrite (no2-) on dissimilatory iron (fe3+) reduction were examined in a series of electron acceptor competition experiments using shewanella putrefaciens 200 as a model iron-reducing microorganism. s. putrefaciens 200 was found to express low-rate nitrate reductase, nitrite reductase, and ferrireductase activity after growth under highly aerobic conditions and greatly elevated rates of each reductase activity after growth under microaerobic condition ...19921548235
isolation and characterization of shewanella alga from human clinical specimens and emendation of the description of s. alga simidu et al., 1990, 335.genetic and phenotypic studies on the strains biochemically identified as shewanella putrefaciens, which had a g+c content ranging from 52 to 54 mol% were conducted. the moles percent g+c of the type strain of s. putrefaciens is 46. surprisingly, dna homology experiments revealed that all these strains are genetically related to shewanella alga (which was reported to produce tetrodotoxin), not to the type strain of s. putrefaciens. in this study, we reidentified clinical strains of s. putrefacie ...19921390113
isolation and characterization of a transposon mutant of shewanella putrefaciens mr-1 deficient in fumarate reductase.a transposon mutant, designated cmtn-3, of shewanella putrefaciens mr-1 that was deficient in fumarate reduction was isolated and characterized. in contrast to the wild-type, cmtn-3 could not grow anaerobically with fumarate as the electron acceptor, and it lacked benzyl viologen-linked fumarate reductase activity. consistent with this, cmtn-3 lacked a 65 kda c-type cytochrome, which is the same size as the fumarate reductase enzyme. cmtn-3 retained the wild-type ability to use nitrate, iron(iii ...19979351256
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