| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| the phylogeny of purple bacteria: the alpha subdivision. | the technique of oligonucleotide cataloging shows the purple photosynthetic eubacteria to comprise three major subdivisions, temporarily called alpha, beta, and gamma--previously designated groups i-iii by gibson et al. (1979). each subdivision contains a number of non-photosynthetic genera in addition to the photosynthetic ones. the alpha subdivision, the subject of the present report, contains most but not all of the species that fall into the classically defined genera rhodospirillum, rhodo ... | 1984 | 11541974 |
| complete genome of phenylobacterium zucineum--a novel facultative intracellular bacterium isolated from human erythroleukemia cell line k562. | phenylobacterium zucineum is a recently identified facultative intracellular species isolated from the human leukemia cell line k562. unlike the known intracellular pathogens, p. zucineum maintains a stable association with its host cell without affecting the growth and morphology of the latter. | 2008 | 18700039 |
| bacterial communities constructed in artificial consortia of bacteria and chlorella vulgaris. | we established artificial consortia of bacteria inoculated from soil and a green alga, chlorella vulgaris nies-227. the bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. the community of bacteria formed in the consortia included those phylogenetically closely related to the genera phenylobacterium, brevundimonas, phyllobacterium, afipia, sphingomonas, sandaracinobacter, ramlibacter, ralstonia, ce ... | 2010 | 21576850 |
| phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus phenylobacterium. | a facultative anaerobic bacterium, strain faii3(t), was isolated from samples collected from the free-flowing waters of a bore well (fairlea bore, registration number 3768) which taps into the australian great artesian basin subsurface thermal aquifer. strain faii3(t) developed yellow to pale-yellow colonies (0.5-1.5 mm) after 48 h. the non-spore forming rods (0.5x1-3 microm) were slightly curved, occurred singly and as pairs and were motile with a single polar flagellum. cells tended to form cl ... | 2004 | 15545448 |
| bacterial degradation of aminopyrine. | 1. four strains of bacteria growing with aminopyrine as sole source of carbon were isolated from soil and were identified as strains of phenylobacterium immobilis. 2. strain m13 and strain e, the type species of phenylobacterium immobilis (dsm 1986), which had been isolated by enrichment with chloridazon (5-amino-4-chloro-2-phenyl-2h-pyridazin-3-one) were used to investigate the bacterial degradation of aminopyrine. 3. three metabolites were isolated and identified as: 4-(dimethylamino)-1,2-dihy ... | 1981 | 7336755 |
| a novel non-heme iron-containing dioxygenase. chloridazon-catechol dioxygenase from phenylobacterium immobilis dsm 1986. | previously we purified an enzyme from phenylobacterium immobilis dsm 1986, which cleaves the catechol derivative of the herbicide chloridazon [5-amino-4-chloro-2-phenyl-3 (2h)-pyridazinone] in the meta position. the enzyme, which could be crystallized, proved in ouchterlony double-diffusion tests to consist of a single protein species. no cross-reaction was observed with other meta-cleaving enzymes. its light absorption spectrum showed a maximum at 279 nm (epsilon = 310 mm -1 cm -1), shoulders a ... | 1982 | 6811270 |
| structural studies on the core oligosaccharide of phenylobacterium immobile strain k2 lipopolysaccharide. chemical synthesis of 3-hydroxy-5c-dodecenoic acid. | the fatty acid composition of the bound lipids of 17 strains from phenylobacterium immobile was investigated. ester-linked 3-hydroxy-5c-dodecenoic acid was found to be the major substituent in the lipopolysaccharide. therefore the occurrence of this unusual acid can be taken as a useful marker for the determination of p. immobile by simple fatty acid analysis. lipid a backbone was found to consist of 2,3-diamino-2,3-dideoxy-d-glucose, which up to this time has only been detected in group i of pu ... | 1985 | 4026999 |
| purification of arogenate dehydrogenase from phenylobacterium immobile. | phenylobacterium immobile, a bacterium which is able to degrade the herbicide chloridazon, utilizes for l-tyrosine synthesis arogenate as an obligatory intermediate which is converted in the final biosynthetic step by a dehydrogenase to tyrosine. this enzyme, the arogenate dehydrogenase, has been purified for the first time in a 5-step procedure to homogeneity as confirmed by electrophoresis. the mr of the enzyme that consists of two identical subunits amounts to 69000 as established by gel elec ... | 1985 | 3967752 |
| purification and n-terminal amino-acid sequences of bacterial malate dehydrogenases from six actinomycetales strains and from phenylobacterium immobile, strain e. | malate dehydrogenases from streptosporangium roseum (dsm 43021), planomonospora venezuelensis (dsm 43178), microtetraspora glauca (atcc 23057), actinoplanes missouriensis (dsm 43046), streptomyces atratus (atcc 14046), kibdelosporangium aridum (atcc 39323), and from phenylobacterium immobile, strain e (dsm 1986) were purified to homogeneity. the n-terminal amino-acid sequences were determined and compared with known prokaryotic and eukaryotic sequence data. the partial sequences from actinomycet ... | 1989 | 2775496 |
| [microbial degradation of aromatic compounds]. | benzene degrading bacteria can be isolated easily. in this contribution, the degradation pathway of benzene has been described. it shows a formation of catechol as an intermediate product which can be split oxidatively between hydroxyl groups (an ortho-splitting) as well as beside the hydroxyl groups (a meta-splitting). the participation of plasmids in the degradation process has been brought out. the herbicide pyraminr becomes metabolized by phenylobacterium immobile in a way which is typical f ... | 1989 | 2658038 |
| phenylobacterium muchangponense sp. nov., isolated from beach soil, and emended description of the genus phenylobacterium. | a gram-staining-negative, non-motile, and aerobic bacterium, designated a8(t) was isolated from the beach soil of muchangpo, korea. cells were rod shaped (0.5-0.6 x 0.7-1.3 ++m) and colonies were colorless, circular with entire edges and had glistening surface. the isolate grew optimally at 25-35-¦c and did not require nacl for growth. strain a8(t) could not assimilate acetate, dl-lactate, succinate, antipyrin and chloridazon, but weakly assimilate l-phenylalanine. major fatty acids were summed ... | 2011 | 21669924 |
| molecular analysis of bacterial communities in uranium ores and surrounding soils from banduhurang open cast uranium mine, india: a comparative study. | bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16s rrna gene based clone library analysis and denaturing gradient gel electrophoresis (dgge) to provide baseline microbial diversity data on autochthonous communities. sequence analysis of major ribotypes and/or dgge bands revealed proteobacteria and acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of ... | 2011 | 21308598 |
| biodegradation of a biocide (cu-n-cyclohexyldiazenium dioxide) component of a wood preservative by a defined soil bacterial community. | the wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. one of these is cu-hdo, containing the chelation product of copper and n-cyclohexyldiazenium dioxide (hdo). in this study, the fate of isotope-labeled ((13)c) and nonlabeled ((12)c) cu-hdo incorporated in wood sawdust mixed with soil was investigated. hdo concentration was monitored by high-pressure liq ... | 2010 | 20952650 |
| a new esterase estd2 isolated from plant rhizosphere soil metagenome. | soil metagenome constitutes a reservoir for discovering novel enzymes from the unculturable microbial diversity. from three plant rhizosphere metagenomic libraries comprising a total of 142,900 members of recombinant plasmids, we obtained 14 recombinant fosmids that exhibited lipolytic activity. a selected recombinant plasmid, pflp-2, which showed maximum lipolytic activity, was further analyzed. dna sequence analysis of the subclone in puc119, pelp-2, revealed an open reading frame of 1,191 bp ... | 2010 | 20683720 |
| cutaneous infectious granuloma caused by phenylobacterium in an adult with myelodysplastic syndrome: a first case report. | painful granulomatous lesions appeared on the face of a 36-year-old man with myelodysplastic syndrome. skin biopsy revealed chronic inflammatory granuloma. bacterial cultures of the lesions and blood indicated the same unknown gram-negative rod bacterium. the 16s ribosomal rna sequence of the unknown bacterium yielded phenylobacterium. thus, we diagnosed cutaneous infectious granuloma caused by phenylobacterium and myelodysplastic syndrome/refractory cytopenia with multi-lineage dysplasia. after ... | 2010 | 20394459 |
| influence of linear alkylbenzene sulfonate (las) on the structure of alphaproteobacteria, actinobacteria, and acidobacteria communities in a soil microcosm. | linear alkylbenzene sulfonate (las) is the most used anionic surfactant in a worldwide scale and is considered a high-priority pollutant. las is regarded as a readily biodegradable product under aerobic conditions in aqueous media and is mostly removed in wastewater treatment plants, but an important fraction (20-25%) is immobilized in sewage sludge and persists under anoxic conditions. due to the application of the sludge as a fertilizer, las reaches agricultural soil, and therefore, microbial ... | 2010 | 19484283 |
| [genome-wide identification of exported proteins of the novel bacterium phenylobacterium zucineum hlk1(t) using a consensus computational strategy]. | to predict the exported proteins of the novel bacterium phenylobacterium zucineum hlk1(t) using genome-wide computational identification by searching the export signals including n-terminal signal peptides and alpha-transmembrane helices. | 2009 | 19363826 |
| phenylobacterium composti sp. nov., isolated from cotton waste compost in korea. | a light-yellow-coloured bacterium, designated strain 4t-6(t), was isolated from cotton waste compost in korea. cells of the strain were strictly aerobic, gram-negative, motile (by means of single polar flagella) and rod-shaped. optimal growth occurred at 30 degrees c and at ph 7.0-8.0. the major fatty acids were 11-methyl c(18 : 1)omega7c (23.8 %), c(18 : 1)omega7c (23.2 %), c(16 : 0) (19.8 %) and c(17 : 0) (14.8 %). the dna g+c content was 67.5 mol%. a phylogenetic analysis based on 16s rrna ge ... | 2008 | 18842845 |
| phylogeny by a polyphasic approach of the order caulobacterales, proposal of caulobacter mirabilis sp. nov., phenylobacterium haematophilum sp. nov. and phenylobacterium conjunctum sp. nov., and emendation of the genus phenylobacterium. | three strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from fresh water and human blood. as determined by analyses of 16s rrna gene sequences, the prosthecate strain fwc 38t was affiliated to the alphaproteobacterial genus caulobacter, with caulobacter henricii (96.8 %) and caulobacter fusiformis (96.8 %) as its closest relatives. the non-prosthecate strain lmg 11050t and the prosthecate strain fwc 21t both belonged to the genus phenylobacterium with phenylobacteriu ... | 2008 | 18676485 |
| [the structural diversity of lipid a from gram-negative bacteria]. | the majority of gram-negative bacteria are pathogenic to humans and animals. lipopolysaccharide (lps) is the most biologically active component of these microorganisms. this compound is also called endotoxin to emphasize its negative impact on a macroorganism. lipid a, one of the three structural components of the lps molecule, is responsible for the pathophysiological effects associated with gram-negative bacteria infections. although lipid a is considered the conservative component of endotoxi ... | 2007 | 17369779 |
| phenylobacterium zucineum sp. nov., a facultative intracellular bacterium isolated from a human erythroleukemia cell line k562. | a bacterial strain hlk1(t) was isolated from the human erythroleukemia cell line k562. this bacterium is a gram-negative rod, motile with a polar flagellum. it is strictly aerobic, nonfermentative, and oxidase and catalase positive. its optimal growth occurs at 37 degrees c at ph between 6.5 and 7.5. phylogenetically, although it shares 98% similarity with the 16s rrna of phenylobacterium lituiforme, the dna-dna hybridization value between the two species is only 43%. hlk1(t) has a dna g+c conte ... | 2007 | 16908113 |
| [a new species of the genus phenylobacterium for the degradation of las (linear alkylbenzene sulfonate)]. | a strain gz6 that can biodegrade las (linear alkylbenzene sulphonate) is identified. it is aerobic gram-negative rod or short-rod (0.5 to 0.8 by 1.0 to 2.0 mm). it is mobile with a single polar flagellum. optimum growth occurred at 30 degrees c and ph7.0. it is catalase positive, urease positive, and arginine decarboxylase positive. all the other physiological and biochemical tests performed were negative. it utilizes the xenobiotic compounds chloridazon, antipyrin and las as sole carbon sources ... | 2003 | 16276864 |
| phenylobacterium koreense sp. nov., isolated from south korea. | a gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, slu-01(t), was isolated from activated sludge from a wastewater treatment plant in daejeon, south korea. after 3 days on r2a medium, strain slu-01(t) developed colourless colonies (0.7-1.2 mm). the non-motile rods (0.7-1.0 x 1.0-2.0 microm) were slightly curved and occurred singly and in pairs. no filamentous cells were found. on the basis of 16s rrna gene sequence similarity, strain slu-01(t) was shown to belong to the family ca ... | 2005 | 16166702 |
| phenylobacterium falsum sp. nov., an alphaproteobacterium isolated from a nonsaline alkaline groundwater, and emended description of the genus phenylobacterium. | a gram-negative bacterium designated ac-49t was isolated from an alkaline groundwater with a ph 11.4. this organism formed rod-shaped cells, was strictly aerobic, catalase and oxidase positive, with an optimum growth temperature of 35 degrees c and an optimum ph value of 8.0. strain ac-49t assimilated primarily amino acids and some krebs cycle metabolites, did not use sugars for growth. the organism did not grow on l-phenylalanine or antipyrin. the g+ c content of dna was 66.9 mol%. the phylogen ... | 2005 | 15997701 |