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isolation and phylogenetic analysis of batai virus, germany.a molecular survey including 16,057 mosquitoes captured in southwest germany during the summer of 2009 showed the presence of batai virus (batv) in anopheles maculipennis sensu lato. until this survey, there was no evidence for circulation of batv in germany. analysis of partial s, m, and l segments showed that the sequences from all three segments were most closely related to batv, indicating that the virus has not undergone reassortment. phylogenetic analysis revealed a close relationship of t ...201121292892
arboviral survey of mosquitoes in two northern italian regions in 2007 and 2008.recently, italy-particularly the emilia-romagna region-was the location of consecutive outbreaks of human diseases caused by the arboviruses chikungunya virus and west nile virus. the two outbreaks, spread by different species of mosquitoes, were not related, but pointed out the lack of an arboviral surveillance program in this region. beginning in 2007 entomological surveillance was initiated in the emilia-romagna region, and in 2008 the program was improved and extended at lombardia region. us ...201020370434
rapid detection of european orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays.the development of reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assays are described herein for the detection of two orthobunyaviruses (bunyaviridae), which represent the two main serogroups found in mosquitoes in central europe. the rt-lamp assays were optimized for the detection of ťahyňa virus (a california encephalitis group virus found in aedes sp or ochlerotatus sp mosquitoes) and batai virus (also called čalovo virus, a bunyamwera group virus found in anopheles m ...201627491341
isolation and full genomic characterization of batai virus from mosquitoes, italy 2009.in 2009, 2589 mosquitoes were collected in northwest italy and screened for orthobunyavirus rna by rt-pcr. one pool of anopheles maculipennis complex mosquitoes was found to be positive and a virus was isolated from that pool. the isolate was identified as batai virus (batv) by sequencing. previously, batv was detected in italy, but limited data and no prior isolates existed. full-length sequences of the s, m and l segments were determined for the newly isolated italian strain. for comparison, p ...201323515020
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