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culicoides: biological vectors of akabane virus.akabane virus replicated in culicoides nubeculosus and culicoides variipennis after intrathoracic inoculation and was maintained in both species of midge for at least 9 days post-infection. the virus also replicated to high concentration in c. variipennis after oral infection and was transmitted through a membrane by this species of midge 7-10 days after infection. the experiments described in this paper provided the first definitive evidence that culicoides spp. are able to act as fully compete ...19892609498
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