| [isolation and identification of arbovirus in hainan province, 2017-2018]. | objective: to understand the types and distribution of arboviruses in hainan province. methods: blood-sucking insects were collected in hainan province from 2017 to 2018. after laboratory treatment, bhk-21 cells and c6/36 cells were inoculated with grinding supernatant of all blood-sucking insects to isolate all of involving virus. arbovirus genes in blood-sucking insects were detected in parallel by rt-pcr method. results: a total of 15 062 mosquitoes were classified into four genera (culex, ar ... | 2020 | 32164136 |
| [isolation and identification of the akabane virus from mosquitoes in yunnan province, china]. | to evaluate the prevalence of mosquito-borne viruses in manshi and ruili (yunnan province, china), we collected 2 149 mosquitoes (17 species) in august 2010. virus isolation was undertaken by the cul- ture of baby hamster kidney cells (bhk-21 cells). two virus-like isolates were obtained: dhl10m117 was isolated from collected in mangshi; dhl10m110 was obtained from anopheles vagus collected in rui- li. both isolates caused cytopathic effects,illness and death in suckling mice inoculated with the ... | 2015 | 25997331 |