expansion of family reoviridae to include nine-segmented dsrna viruses: isolation and characterization of a new virus designated aedes pseudoscutellaris reovirus assigned to a proposed genus (dinovernavirus). | family reoviridae is known, by definition, to contain dsrna viruses with 10-12 genome segments. we report here the characterization of the first member of this family with a nine-segmented genome. this virus was isolated from aedes pseudoscutellaris mosquito cells and designated aedes pseudoscutellaris reovirus (aprv). virions are single-shelled with turrets but are non-occluded by contrast to cypoviruses. aprv replicates in various mosquito cell lines, but not in mice or mammalian cells. comple ... | 2005 | 16171838 |
bioinformatic analysis suggests that the cypovirus 1 major core protein cistron harbours an overlapping gene. | members of the genus cypovirus (family reoviridae) are common pathogens of insects. these viruses have linear dsrna genomes divided into 10-11 segments, which have generally been assumed to be monocistronic. here, bioinformatic evidence is presented for a short overlapping coding sequence (cds) in the cypovirus genome segment encoding the major core capsid protein vp1, overlapping the 5'-terminal region of the vp1 orf in the +1 reading frame. in cypovirus type 1 (cpv-1), a 62-codon aug-initiated ... | 2008 | 18492230 |
characterization of the complete genome segments from bmcpv-sz, a novel bombyx mori cypovirus 1 isolate. | a novel bombyx mori cypovirus 1 isolated from infected silkworm larvae and tentatively assigned as bombyx mori cypovirus 1 isolate suzhou (bmcpv-sz). the complete nucleotide sequences of genomic segments s1-s10 from bmcpv-sz were determined. all segments possessed a single open reading frame; however, bioinformatic evidence suggested a short overlapping coding sequence in s1. each bmcpv-sz segment possessed the conserved terminal sequences aguaa and guuagcc at the 5' and 3' ends, respectively. t ... | 2012 | 22712678 |