| development and utilization of complement-fixation and immune adherence tests for human hepatitis a virus and antibody. | the reliable propagation of cr326 strain of human hepatitis a virus in saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (cf), and immune adherence (ia) assays for hepatitis a antigen and antibody. the cf and ia assay were made possible by the use of livers of cr326-infected marmosets as a source of hepatitis a antigen. all assays were shown to be specific for hepatitis a. patients with hepatitis b did not show development of hepatitis ... | 1975 | 53013 |
| immune adherence and complement-fixation tests for human hepatitis a. diagnostic and epidemiologic investigations. | the reliable propagation of cr326 strain of human hepatitis a virus in saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (cf), and immune adherence (ia) assays for hepatitis a antigen and antibody. the cf and ia assays were made possible by the use of livers of cr326-infected marmosets as a source of hepatitis a antigen. all assays were shown to be specific for hepatitis a. cases of hepatitis b did not show development of hepatitis a an ... | 1975 | 173600 |
| localization of hepatitis a antigen in marmoset organs during acute infection with hepatitis a virus. | twelve marmosets (saguinus mystax) were inoculated intravenously (iv) with hepatitis a virus (hav). one died early (day 12); seven were sacrificed at the time of elevation in level of alanine aminotransferase (serum glutamic-pyruvic transaminase), and four without elevation were not sacrificed but seroconverted. in the seven marmosets sacrificed during the acute stage of illness, hepatitis a antigen (ha ag) was detected in the liver by immunofluorescence in all cases, by immune electron microsco ... | 1978 | 212488 |
| [the modelling of hepatitis a and of enterally transmitted non-a, non-b hepatitis (hepatitis e) in saguinus mystax tamarins]. | a comparative study of hepatitis infection caused by human hepatitis a virus (ms-1 strain), simian hepatitis a virus (agm-27 strain), and enterically transmitted non-a, non-b hepatitis virus (tashkent-1435 strain) was carried out. susceptibility of tamarins to the agm-27 and tashkent-1435 as well as to ms-1 strain was demonstrated. all the strains induced an acute infection characterized by serum alanine aminotransferase (alt) elevation, virus excretion and antibody response. certain differences ... | 1990 | 1967121 |
| infectivity studies of hepatitis a and b in non-human primates. | we have induced hepatitis a in marmosets of the subspecies saguinus mystax following primary inoculation with human serum containing the ms-1 strain of hepatitis a virus (hav) and in 3 further marmoset subpassages using infective marmoset serum from each preceding passage. in each passage acquisition of serum antibody against 17 nm virus-like particles recovered from acute illness stools of human volunteers who developed hepatitis following inoculation with the ms-1 strain of hav, as well as fro ... | 1975 | 173599 |
| utilization of chimeras between human (hm-175) and simian (agm-27) strains of hepatitis a virus to study the molecular basis of virulence. | chimeras between human (hm-175) and simian (agm-27) strains of hepatitis a virus (hav) were constructed to evaluate the effect of the 2c gene of agm-27 on hav replication in cell culture and virulence in tamarins (saguinus mystax) and chimpanzees (pan troglodytes). kinetic studies and radioimmunofocus assays demonstrated that replacement of the 2c gene of hav/7, a cell culture-adapted strain of hm-175, with that of agm-27 drastically reduced the ability of the virus to replicate in cultured cell ... | 1998 | 9696843 |
| [experimental models of hepatitis a in macaques using viral strains isolated from man and monkeys]. | experimental hepatitis a (ha) models were obtained in macaca monkeys (15 m. fascicularis and 4 m. mulatta) by means of the strains of hepatitis a virus (hav) isolated from the feces of a patient (hav-h) and of spontaneously infected m. mulatta (hav-mm) and green monkeys cercopithecus aethiops (hav-ca). irrespective of the strains used all seronegative macaca monkeys developed ha after intravenous-oral inoculation with the following patterns: elevation of the serum alanine aminotransferase level, ... | 1994 | 8087252 |
| a hepatitis a virus deletion mutant which lacks the first pyrimidine-rich tract of the 5' nontranslated rna remains virulent in primates after direct intrahepatic nucleic acid transfection. | cell culture-adapted variants of hepatitis a virus (hav) in which the first pyrimidine-rich tract (py1; nucleotides 99 to 138) of the 5' nontranslated region has been deleted (delta 96-137 or delta 96-139) replicate as well as parental virus in cultured cells (d.r. shaffer, e.a. brown, and s.m. lemon, j. virol. 68:5568-5578, 1994). to determine whether viruses with such large deletion mutations are able to replicate and to produce acute hepatitis in primates, we reconstructed the delta 96-137 de ... | 1995 | 7666566 |
| pathogenetic aspects of hepatitis a virus infection in enterally inoculated marmosets. | experimental hepatitis a virus (hav) infection was studied in marmosets after enteral (intragastric) inoculation with special reference to the primary sites of hav replication and immunopathology of the disease. the experiment was carried out using 28 saguinus mystax negative for antibody to hav (anti-hav) and with statistically uniform baseline values of serum isocitrate dehydrogenase (sicd) activity. each animal was infected with 1 ml of a 15% w/v stool suspension that was derived from marmose ... | 1981 | 6271006 |
| hepatitis a virus in the liver and intestine of marmosets after oral inoculation. | a total of 12 seronegative marmosets (saguinus mystax) were inoculated orally with hepatitis a virus (hav) and sacrificed at 3- to 4-day intervals. tissues from the livers, intestines, mesenteric lymph nodes, and spleens were obtained for immunofluorescence studies, and bile and intestinal contents were obtained for enzyme-linked immunosorbent assay studies. two marmosets sacrificed on days 34 and 41 after inoculation developed antibody to hav and demonstrated hav in their livers but not in any ... | 1980 | 6247287 |
| [a trial of a cultured inactivated vaccine against hepatitis a on saguinus mystax tamarins]. | an experimental batch of inactivated hepatitis a vaccine was prepared using hepatitis a virus (hav), has-15 strain, adapted to cell culture and purified by ultracentrifugation. the vaccine was tested in tamarins immunized intramuscularly three times one month apart. three tamarins received a vaccine preparation containing 10 ng of immunogen each, three--100 ng each, and three animals were used as controls. the efficacy was judged by the anti-hav antibody response in the vaccinated animals and de ... | 1990 | 2256314 |
| experimental infection of marmosets with hepatitis a virus. | saguinus mystax marmosets were experimentally infected with two strains of human hepatitis a virus. one of these strains of hav was successfully subpassaged in this species of marmosets. in another experiment, the 1.32 and 1.41 g/cm3 buoyant density species of hav derived from an infected chimpanzee stool were shown to be infectious in three species of marmosets. the value of the marmoset as an experimental model for hepatitis a infection was demonstrated by these studies. | 1978 | 205859 |