| reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains. | vaccination is the practiced and accessible measure for preventing influenza infection. because chicken embryos used for vaccine production have various insufficiencies, more efficient methods are needed. african green monkey kidney (vero) cells are recommended by the world health organization (who) as a safe substitute for influenza vaccine production for humans. however, the influenza virus usually had low-yield in vero cells, which limits the usage of vero cellular vaccines. this study used 2 ... | 2017 | 27648636 |
| generation of high-yielding influenza a viruses in african green monkey kidney (vero) cells by reverse genetics. | influenza a viruses are the cause of annual epidemics of human disease with occasional outbreaks of pandemic proportions. the zoonotic nature of the disease and the vast viral reservoirs in the aquatic birds of the world mean that influenza will not easily be eradicated and that vaccines will continue to be needed. recent technological advances in reverse genetics methods and limitations of the conventional production of vaccines by using eggs have led to a push to develop cell-based strategies ... | 2004 | 14747549 |
| receptor-binding properties of modern human influenza viruses primarily isolated in vero and mdck cells and chicken embryonated eggs. | to study the receptor specificity of modern human influenza h1n1 and h3n2 viruses, the analogs of natural receptors, namely sialyloligosaccharides conjugated with high molecular weight (about 1500 kda) polyacrylamide as biotinylated and label-free probes, have been used. viruses isolated from clinical specimens were grown in african green monkey kidney (vero) or madin-darby canine kidney (mdck) cells and chicken embryonated eggs. all vero-derived viruses had hemagglutinin (ha) sequences indistin ... | 2003 | 12954214 |
| amino acid substitution at position 226 of the hemagglutinin molecule of influenza (h1n1) virus affects receptor binding activity but not fusion activity. | the receptor binding site of the hemagglutinin (ha) molecule of type a influenza virus a/ussr/90/77 (h1n1) has been studied. site-specific mutagenesis has been used to introduce base changes into the sequence that codes for the amino acid residue at position 226 on the ha molecule, and mutant sequences replaced the wild-type sequence of the ha gene of the sv40-ha recombinant virus (svha). mutant ha proteins were expressed in african green monkey kidney cells and analyzed for receptor binding and ... | 1988 | 2460997 |
| impact of host cell line adaptation on quasispecies composition and glycosylation of influenza a virus hemagglutinin. | the genome of influenza a viruses is constantly changing (genetic drift) resulting in small, gradual changes in viral proteins. alterations within antibody recognition sites of the viral membrane glycoproteins hemagglutinin (ha) and neuraminidase (na) result in an antigenetic drift, which requires the seasonal update of human influenza virus vaccines. generally, virus adaptation is necessary to obtain sufficiently high virus yields in cell culture-derived vaccine manufacturing. in this study det ... | 2011 | 22163276 |
| live cold-adapted influenza a vaccine produced in vero cell line. | the african green monkey kidney (vero) cell line was used as a substrate for the development of a live cold-adapted (ca) reassortant influenza vaccine. for that purpose, a new master strain was generated by an adaptation of the wild type (wt) a/singapore/1/57 virus to growth at 25 degrees c in a vero cell line. the resulting cold-adapted (ca) muster strain a/singapore/1/57ca showed temperature sensitive (ts) phenotype and was attenuated in animal models and protective in the challenge experiment ... | 2004 | 15163508 |
| evaluation of replication, immunogenicity and protective efficacy of a live attenuated cold-adapted pandemic h1n1 influenza virus vaccine in non-human primates. | we studied the replication of influenza a/california/07/09 (h1n1) wild type (ca09wt) virus in two non-human primate species and used one of these models to evaluate the immunogenicity and protective efficacy of a live attenuated cold-adapted vaccine, which contains the hemagglutinin and neuraminidase from the h1n1 wild type (wt) virus and six internal protein gene segments of the a/ann arbor/6/60 cold-adapted (ca) master donor virus. we infected african green monkeys (agms) and rhesus macaques w ... | 2012 | 22789506 |
| development of high-yield influenza a virus vaccine viruses. | vaccination is one of the most cost-effective ways to prevent infection. influenza vaccines propagated in cultured cells are approved for use in humans, but their yields are often suboptimal. here, we screened a/puerto rico/8/34 (pr8) virus mutant libraries to develop vaccine backbones (defined here as the six viral rna segments not encoding haemagglutinin and neuraminidase) that support high yield in cell culture. we also tested mutations in the coding and regulatory regions of the virus, and c ... | 2015 | 26334134 |