| differentiation of pathogenic and non-pathogenic serotype 1 marek's disease viruses (mdvs) by the polymerase chain reaction amplification of the tandem direct repeats within the mdv genome. | there are no simple, direct methods to reliably distinguish oncogenic serotype 1 marek's disease viruses (mdvs) from their attenuated variants. the present study was an attempt to apply polymerase chain reaction (pcr) to develop a rapid and sensitive assay for the presence of the mdv genome. pcr oligos were chosen to flank the 132-base-pair tandem direct repeats in the serotype 1 mdv genome. the pcr reaction was specific for serotype 1 mdvs, amplifying fragments corresponding to one to three cop ... | 1992 | 1329706 |
| monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses. | an enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ... | 1988 | 2838016 |
| an igm-producing b lymphoblastoid cell line established from lymphomas induced by a non-defective reticuloendotheliosis virus. | chick syncytial virus (csv), a strain of avian reticuloendotheliosis virus (rev) causes lymphoid tumours in chickens after a prolonged incubation period. a number of csv-induced tumours were examined for cell surface antigen and were found to be of the b cell type and to produce immunoglobulin. attempts were made to grow in vitro cell lines from csv-induced tumours and a lymphoblastoid cell line was established from a liver tumour of a chicken that was inoculated with csv via the yolk sac in emb ... | 1982 | 6278061 |
| effect of endogenous leukosis virus genes on response to infection with avian leukosis and reticuloendotheliosis viruses. | we examined the effect of the presence or absence of endogenous viral gene (ev) 3, which controls expression of group-specific viral and envelope antigens (gs+chf+ phenotype), and ev2, which controls the production of a complete subgroup e virus (v-e+ phenotype), on the response of chickens to rav-1, an exogenous avian leukosis virus (alv) with an antigenic relationship to endogenous virus. after inoculation at one day of age, the chickens lacking either ev gene expression had a lower frequency ... | 1982 | 6285878 |
| stimulation of local solid tumour development of the nonproducer marek's disease tumour transplant jmv by virus-induced immunosuppression. | chickens could be protected against lethal lymphoblastic leukaemia due to the nonproducer jmv marek's disease (md) tumour transplant by infection with the herpesvirus of turkeys (hvt) or various strains of md virus. however, solid jmv tumours developed in md virus-infected birds at the site of intramuscular or subcutaneous transplantation, but tumours never developed at the site of md virus inoculation. the incidence and extent of local tumour growth, the development of metastases and the inhibi ... | 1980 | 18770243 |
| immunological effects of reticuloendotheliosis virus as potential contaminant of marek's disease vaccines. | certain properties of four strains of reticuloendotheliosis virus (rev), namely rev-f, rev-s, rev-c and chick syncytial virus (csv) were studied in vivo and in vitro. in chicken embryo fibroblast (cef) cultures these viruses caused a chronic infection which could be detected by indirect immunofluorescence (fa) tests. precipitating antigens were present in infected cultured cells as well as in culture supernatants. antibodies in sera of rev-inoculated chickens were demonstrated by fa tests and ag ... | 1977 | 18770347 |