| quantitation and localization of rous sarcoma virus-specific rna in transformed and revertant field vole cells. | hybridization analysis of rna from transformed clones of rous sarcoma virus (rsv)-infected field vole cells and revertant subclones indicated the presence of similar amounts of viral-specific rna in both cell types. employing both a relatively uniform and representative complementary dna probe and genomelength complementary dna, we have demonstrated that the majority of rsv proviral dna is transcribed into viral-specific rna in both transformed and revertant clones. the viral-specific rna is pre ... | 1978 | 203719 |
| nature of rous sarcoma virus-specific rna in transformed and revertant field vole cells. | cytoplasmic and polyribosomal rnas from rous sarcoma virus-transformed and phenotypically reverted field vole cells were fractionated by rate-zonal sedimentation and hybridized with a (3)h-labeled complementary dna viral probe to determine the size classes of virus-specific rna present in these cell types. in contrast to rous sarcoma virus-infected permissive avian cells, only two of three discrete species of virus-specific rna were detected in the cytoplasm of these vole cells. these included g ... | 1979 | 219246 |
| morphological revertants of an avian sarcoma virus-transformed mammalian cell line exhibit tumorigenicity and contain pp60src. | the biological and biochemical properties of rous sarcoma virus-transformed and revertant field vole cells were investigated. revertant vole cells appear morphologically similar to normal, uninfected cells, yet, like transformed vole cells, they are fully capable of growing in agar suspension and producing tumors in athymic nude mice. these highly tumorigenic, yet morphologically normal appearing, vole cells express viral-specific antigens such as the gag gene product (pr76) but lack the env gen ... | 1979 | 226988 |
| highly specific antibody to rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules. | antiserum to the rous sarcoma virus (rsv)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (tbr) sera. when rsv-transformed cell lysates were preadsorbed with tbr serum, the remaining lysate contained additional pp60v-src, which was recognized only by reimmunoprecipitation with alpha p60 serum and not by tbr serum. in subcellular fractions of rs ... | 1985 | 2981886 |
| the induction of tumours with rous sarcoma virus in microtus agrestis: a preliminary note. | | 1970 | 4325786 |
| karyotype analysis and quantitation of viral transforming genes in rous sarcoma virus transformed, revertant, and retransformed field vole cells. | comparative studies of the number of cellular chromosomes and viral genes, including the gene required for malignant transformation, were performed on several clones of rous sarcoma virus-transformed, revertant, and spontaneously retransformed field vole cells. the results of these studies indicate that no appreciable differences in either total viral gene equivalents or transforming gene sequences can be detected between transformed and revertant cell types, even though considerable differences ... | 1981 | 6262017 |
| increase in the phosphotransferase specific activity of purified rous sarcoma virus pp60v-src protein after incubation with atp plus mg2+. | pp60v-src, the product of the rous sarcoma virus src gene, was partially purified by immunoaffinity chromatography from extracts of rous sarcoma virus-transformed field vole cells. incubation of this preparation with atp plus mg2+ and subsequent repurification by chromatography on hexylamine-agarose resulted in a net increase in the specific activity of the src protein kinase. this increase in phosphotransferase activity was detected by using a variety of substrates including casein, tubulin, an ... | 1983 | 6314123 |