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multiplex pcr assay for the detection of anopheles fluviatilis species complex, human host preference, and plasmodium falciparum sporozoite presence, using a unique mosquito processing method.a multiplex pcr assay has been developed for detection of anopheles fluviatilis cryptic species, their human host preference, and plasmodium falciparum presence in the mosquito. pcr conditions were optimized using primer sets specific for a. fluviatilis cryptic species, homo sapiens, and p. falciparum and evaluated with field-collected mosquitoes. a unique mosquito processing method was used for screening p. falciparum carrying capacity and human host preference of a. fluviatilis mosquitoes in f ...200717488901
differentiation of members of the anopheles fluviatilis species complex by an allele-specific polymerase chain reaction based on 28s ribosomal dna sequences.anopheles fluviatilis, one of the major vectors of malaria in india, is a complex of at least three cryptic species provisionally designated as species s, t, and u. identification of the cryptic species of an. fluviatilis complex is of paramount importance in disease control program due to contrasting differences in their vectorial efficiency, preference for feeding on humans, and resting behavior. species s, t, and u are morphologically indistinguishable at any stage of their life cycle and can ...200414971694
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