| isolation of spotted fever group and wolbachia-like agents from field-collected materials by means of plaque formation in mammalian and mosquito cells. | three isolations from ticks (dermacentor occidentalis) of a rickettsia of the spotted fever group and 5 isolations from chipmunk (eutamias rugicaudus) blood of a wolbachia-like agent were obtained from plaques formed in singh's aedes albopictus (mosquito) and vero (african green monkey kidney) cell cultures. these organisms could not be isolated by injection of the infected ticks or blood into embryonated chicken eggs, guinea pigs, or voles (microtus pennsylvanicus), but fluid cultures of grace' ... | 1975 | 241251 |
| selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. i. attributes of uncloned virus at different passage levels. | attempts were made to attenuate prototype dengue (den) 4 (h-241) virus. the original viremic human serum was passed once in a susceptible monkey and twice in aedes albopictus mosquitoes and then serially passed in primary dog kidney (pdk) and african green monkey kidney (gmk) cells. weekly transfers of undiluted virus were carried to the 50th passage in both primary cell cultures. biological markers were studied at passages 7, 15, 30 and 50. parental den-4 phenotype characteristics included larg ... | 1984 | 6476212 |
| stimulation of dengue virus replication in cultured aedes albopictus (c6/36) mosquito cells by the antifungal imidazoles ketoconazole and miconazole. | replication of dengue type 3 virus in aedes albopictus c6/36 cells was enhanced more than 50-fold by addition of the antifungal imidazole derivative ketoconazole within the first 4 h of infection. the stimulatory effect was reflected in the yield of infectious virus and in levels of viral rna and protein synthesis. enhanced yields were observed also for other flaviviruses, including dengue type 2 virus and murray valley encephalitis virus. increased yields of dengue type 3 virus were not observe ... | 2000 | 10725192 |
| c6/36 aedes albopictus cells have a dysfunctional antiviral rna interference response. | mosquitoes rely on rna interference (rnai) as their primary defense against viral infections. to this end, the combination of rnai and invertebrate cell culture systems has become an invaluable tool in studying virus-vector interactions. nevertheless, a recent study failed to detect an active rnai response to west nile virus (wnv) infection in c6/36 (aedes albopictus) cells, a mosquito cell line frequently used to study arthropod-borne viruses (arboviruses). therefore, we sought to determine if ... | 2010 | 21049065 |
| structural investigation of c6/36 and vero cell cultures infected with a brazilian zika virus. | zika virus (zikv) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand rna enclosed in a capsid and surrounded by a membrane. studies on the replication dynamics of zikv are scarce, which limits the development of antiviral agents and vaccines directed against zikv. in this study, aedes albopictus mosquito lineage cells (c6/36 cells) and african green monkey kidney epithelial cells (vero cells) were inoculated with a zikv sample isolated from a b ... | 2017 | 28898286 |
| differential susceptibility & replication potential of vero e6, bhk-21, rd, a-549, c6/36 cells & aedes aegypti mosquitoes to three strains of chikungunya virus. | chikungunya virus (chikv), a mosquito-borne arthritogenic virus causes infections ranging from febrile illness to debilitating polyarthralgia in humans. re-emergence of the virus has affected millions of people in africa and asia since 2004. during the outbreak, a new lineage of the virus has evolved as an adaptation for enhanced replication and transmission by aedes albopictus mosquito. a study was designed to compare the susceptibility of four vertebrate cell lines, namely vero e6 (african gre ... | 2019 | 31496530 |
| evaluation of host systems for efficient isolation and propagation of duck tembusu virus. | several phylogenetic clusters of duck tembusu virus (dtmuv) that caused outbreaks in ducks in asia have been identified since its emergence in 2010, highlighting the need for an efficient host system that can support isolation of all circulating phylogenetic clusters of dtmuv. in this study, various host systems including different avian embryonated eggs (duck and chicken) and cell cultures (primary duck embryo fibroblast (def), primary chicken embryo fibroblast (cef), baby hamster kidney (bhk-2 ... | 2020 | 33146547 |