| insecticide susceptibility of some vector fleas and mosquitoes in burma. | rat fleas and mosquitoes are insect vectors of public health importance in burma. plague is endemic in central burma and ddt has been the principal insecticide used for its control to date. dengue haemorrhagic fever, recently introduced and transmitted by aedes aegypti, has been spreading to major towns since 1971. the rodents, rattus rattus, r. exulans, bandicota bengalensis, mus musculus, as well as shrews were commonly caught during routine trapping in the country. rattus norvegicus, prevalen ... | 1975 | 131975 |
| the putative cell cycle gene, enhancer of rudimentary, encodes a highly conserved protein found in plants and animals. | the enhancer of rudimentary gene, e(r), in drosophila melanogaster encodes a protein, er, whose function has been implicated in pyrimidine biosynthesis and the cell cycle (wojcik et al. (1994) genetics 138, 1163-1170). in order to identify conserved regions of the protein and potentially important functional domains, the e(r) gene was cloned and sequenced from two other insects (drosophila virilis and aedes aegypti) and three vertebrates (homo sapiens, mus musculus, and brachydanio rerio) and se ... | 1997 | 9074495 |
| molecular characterization of a kif3a-like kinesin gene in the testis of the chinese fire-bellied newt cynops orientalis. | kif3a, the subunit within the kinesin-2 superfamily, is a typically n-terminal motor protein, which is involved in membranous organelle and intraflagellar transport. during spermatogenesis, kif3a plays a critical role in the formation of flagella and cilia. kif3a is also related to the left-right asymmetry, the signal pathway, dna damage and tumorigenesis. we used rt-pcr and in situ hybridization to clone the kif3a gene, and we identified its function in the testis of the chinese fire-bellied ne ... | 2011 | 21773941 |
| acetylcholinesterases from the disease vectors aedes aegypti and anopheles gambiae: functional characterization and comparisons with vertebrate orthologues. | mosquitoes of the anopheles (an.) and aedes (ae.) genus are principal vectors of human diseases including malaria, dengue and yellow fever. insecticide-based vector control is an established and important way of preventing transmission of such infections. currently used insecticides can efficiently control mosquito populations, but there are growing concerns about emerging resistance, off-target toxicity and their ability to alter ecosystems. a potential target for the development of insecticide ... | 2015 | 26447952 |
| decoding the ubiquitin-mediated pathway of arthropod disease vectors. | protein regulation by ubiquitin has been extensively described in model organisms. however, characterization of the ubiquitin machinery in disease vectors remains mostly unknown. this fundamental gap in knowledge presents a concern because new therapeutics are needed to control vector-borne diseases, and targeting the ubiquitin machinery as a means for disease intervention has been already adopted in the clinic. in this study, we employed a bioinformatics approach to uncover the ubiquitin-mediat ... | 2013 | 24205097 |
| toxicological evaluation of essential oil from the leaves of croton argyrophyllus (euphorbiaceae) on aedes aegypti (diptera: culicidae) and mus musculus (rodentia: muridae). | plant-derived essential oils can be used as insecticides for vector control. however, to establish their safety, it is necessary to perform toxicological studies. herein, we evaluated the chemical composition and insecticidal activity of the essential oil from the leaves of croton argyrophyllus on the third- and fourth-instar larvae and adult aedes aegypti (l., 1762). we also evaluated the acute toxicity of the essential oil in adult female mus musculus the lethal concentration 50 (lc50) and 90 ... | 2017 | 28132021 |
| toxicological evaluation of essential oil from the leaves of croton tetradenius (euphorbiaceae) on aedes aegypti and mus musculus. | for control of aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of croton tetradenius on ae. aegypti larvae and adults. we also evaluated the acute toxicity in mus musculus. the essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. female mice were used for assessing tox ... | 2016 | 27169864 |
| characterization and expression analysis of prohibitin in the testis of chinese mitten crab eriocheir sinensis. | prohibitin plays a key role in maintaining mitochondrial membrane integrity and retaining its normal function. we have initially cloned and sequenced the cdna of prohibitin from testis of the crab eriocheir sinensis. the 1,357 bp prohibitin cdna comprises a 105 bp 5' untranslated region, a 427 bp 3' untranslated region and a 825 bp open reading frame. protein alignment substantiates that the prohibitin has 70.2, 69.8, 70.5, 70.9, 72.4, 70.6 and 74.9% identity with its homologues in mus musculus, ... | 2012 | 22311031 |
| high throughput discovery of protein variants using proteomics informed by transcriptomics. | proteomics informed by transcriptomics (pit), in which proteomic ms/ms spectra are searched against open reading frames derived from de novo assembled transcripts, can reveal previously unknown translated genomic elements (tges). however, determining which tges are truly novel, which are variants of known proteins, and which are simply artefacts of poor sequence assembly, is challenging. we have designed and implemented an automated solution that classifies putative tges by comparing to referenc ... | 2018 | 29718325 |
| how to turn an organism into a model organism in 10 'easy' steps. | many of the major biological discoveries of the 20th century were made using just six species: escherichia coli bacteria, saccharomyces cerevisiae and schizosaccharomyces pombe yeast, caenorhabditis elegans nematodes, drosophila melanogaster flies and mus musculus mice. our molecular understanding of the cell division cycle, embryonic development, biological clocks and metabolism were all obtained through genetic analysis using these species. yet the 'big 6' did not start out as genetic model or ... | 2020 | 32034051 |